This document provides a reference chart for various analytical parameters including maximum holding times, required container types, required preservatives, and minimum sample volumes. It lists over 50 parameters, including alkalinity, ammonia, anions, asbestos, biochemical oxygen demand, cyanide, dissolved oxygen, hardness, metals, mercury, nitrogen, oil and grease, pesticides, and more. The chart specifies plastic or glass containers, temperature storage requirements like 0-6°C, and any necessary preservatives such as H2SO4 or HNO3. Holding times range from 15 minutes for chlorine residual up to 6 months for some metals analyses.
This document provides a reference chart for various analytical parameters including maximum holding times, required container types, required preservatives, and minimum sample volumes. It lists over 50 parameters, including alkalinity, ammonia, anions, asbestos, biochemical oxygen demand, cyanide, dissolved oxygen, hardness, metals, mercury, nitrogen, oil and grease, pesticides, and more. The chart specifies plastic or glass containers, temperature storage requirements like 0-6°C, and any necessary preservatives such as H2SO4 or HNO3. Holding times range from 15 minutes for chlorine residual up to 6 months for some metals analyses.
This document provides a reference chart for various analytical parameters including maximum holding times, required container types, required preservatives, and minimum sample volumes. It lists over 50 parameters, including alkalinity, ammonia, anions, asbestos, biochemical oxygen demand, cyanide, dissolved oxygen, hardness, metals, mercury, nitrogen, oil and grease, pesticides, and more. The chart specifies plastic or glass containers, temperature storage requirements like 0-6°C, and any necessary preservatives such as H2SO4 or HNO3. Holding times range from 15 minutes for chlorine residual up to 6 months for some metals analyses.
This document provides a reference chart for various analytical parameters including maximum holding times, required container types, required preservatives, and minimum sample volumes. It lists over 50 parameters, including alkalinity, ammonia, anions, asbestos, biochemical oxygen demand, cyanide, dissolved oxygen, hardness, metals, mercury, nitrogen, oil and grease, pesticides, and more. The chart specifies plastic or glass containers, temperature storage requirements like 0-6°C, and any necessary preservatives such as H2SO4 or HNO3. Holding times range from 15 minutes for chlorine residual up to 6 months for some metals analyses.
REFERENCE CHART: HOLDING TIME, BOTTLE TYPE, PRESERVATION, MINIMUM VOLUME
Note: Multiple analyses may be run from the same container, provided that the bottle type, volume and preservation are appropriate. Analytical Parameter Maximum Holding Time Required Required Minimum Container Type Preservative Amount Alkalinity (Bicarb, Carb, Hyd, and Tot); SM 2320 B 14 days Plastic / Glass 0-6°C 100 mL Ammonia (NH3 as N); SM 4500NH3 B/C or B/G 28 days Plastic / Glass H2SO4 pH <2 +0-6°C 200 mL Anions by EPA 300.0: Chloride (Cl-); Fluoride (F-); 28 days Plastic / Glass 0-6° C 100 mL Nitrate (as N); Sulfate (SO42-) 48 hours (Nitrate) Asbestos (TEM) 48 hours Plastic / Glass 0-6°C 1000 mL Bioassay (Acute Tox) % survival 36 hours Plastic cubitainer 0-6°C 1-5 gal Biochemical Oxygen Demand (BOD); SM 5210 B 48 hours Plastic / Glass 0-6°C 250 mL Chromium, Hexavalent (CrVI); SM 3500Cr B 24 hours Plastic / Glass 0-6°C 200 mL Chlorine, residual; SM 4500Cl B or G 15 minutes Field Measurement - - - Chemical Oxygen Demand (COD); SM 5220 D or 28 days Plastic / Glass H2SO4 pH <2 +0-6°C 50 mL EPA 410.4 Chlorophyll A (Algal Biomass) / Pheophytin; SM 48 Hours to filter Amber Plastic 0-6°C, unfiltered 1 L 10200 H 28 days once filtered -20°C, filtered 8 hrs – Plastic (sterile) Na2S2O3 + 0-10°C 100 mL Coliform, Total / Fecal; SM 9221 B/E wastewater/stormwater No regulatory temp. req. 30 hrs – drinking water for drinking water) Coliform, Total / E.Coli; SM 9223 B 30 hrs – drinking water Plastic (sterile) Na2S2O3 + 0-10°C; No 100 mL (Present/Absent or Quantitray) regulatory temp. req. for DW Color; SM 2120 B 48 hours Plastic / Glass 0-6°C 100 mL Conductivity (EC/SC); SM 2510 B 28 days Plastic / Glass 0-6°C 100 mL Amber or opaque plastic with NaOH pH >10+0-6°C 100 mL 14 days Pre-treatment for Total Cyanide samples with chlorine Cyanide Total; SM 4500CN B/C/E or B/C/I or NO3/NO2 should be done prior to preservation. Kits can be provided upon request. Cyanide WAD; SM 4500CN B/C/E or B/C/I 14 days Amber plastic NaOH pH >10+0-6°C Filter within 48 hours 250 mL Amber glass Filter then add HCl to 50 mL Dissolved Organic Carbon (DOC); SM 5310 B 28 days pH< 2 + 0-6°C 15 minutes Field Measurement or 500 mL Dissolved Oxygen (DO); SM 4500O G Glass bottle/no headspace 0-6°C Collect in duplicate Enterococcus by Enterolert 8 hours Plastic (sterile) Na2S2O3 + 0-10°C 100 mL Heterotrophic Plate Count (HPC/SPC); SIMPLATE 8 hours Plastic (sterile) Na2S2O3 + 0-10°C 100 mL Hardness (by titration); SM 2340 C 180 days Plastic / Glass HNO3 pH <2 250 mL Filter within 15 Plastic / Glass Filter then add HNO3 100 mL Metals, Dissolved {2}; EPA 200.8 minutes{2}, 6 months Metals, Total; EPA 200.8 6 months Plastic / Glass HNO3 pH <2 100 mL Metals, Total; EPA 6010/6020 Solids in Glass SJ 0-6°C 1 8oz Mercury (Hg) EPA 245.1 28 days Plastic / Glass HNO3 pH <2 100 mL Mercury (Hg) EPA 7471 Solids in Glass SJ 0-6°C 1 8oz Mercury, Total; EPA 1631 (Aqueous) 48 hours to preserve Glass, Dbl Bagged (not HCl 100 mL 90 days once preserved appropriate for samples with solids TSS>200 mg/L) Mercury, Total; EPA 1631 (Sludge or Solids) 90 days Solids in Glass SJ 0-6°C 1 8oz Filter within 24 hours, Glass, Dbl Bagged Filter in lab, then HCl 100 mL Mercury, Dissolved {2}; EPA 1631 Preserve within 48 hours 90 days once preserved Mercury, Methyl; EPA 1630 (Aqueous) Preserve {8} within 48 hrs Glass, Amber Dbl Bagged Dark and cool + (HCl or 100 mL 6 months preserved H2SO4 {8}) Mercury, Methyl; EPA 1630 (Sludge or Solids) 6 months frozen Solids in Amber Poly SJ Solids to be Frozen 1 8oz Filter & preserve within Glass, Amber Dbl Bagged Filter in Lab, 0-6°C + 100 mL Mercury, Dissolved {2} Methyl; EPA 1630 48 hrs HCl if < 10 ppT salinity 6 months once preserved {8} Nitrate (as N); EPA 300.0 48 hours Plastic / Glass 0-6°C 100 mL Nitrite (as N); SM 4500NO2 B 48 hours Plastic / Glass 0-6°C 100 mL Nitrate+Nitrite as N (NO3+NO2-N) EPA 353.2, or SM 28 days Plastic / Glass H2SO4 pH <2 +0-6°C 100 mL NO3 F Nitrogen, Total Kjeldahl (TKN); SM 4500Norg + SM 28 days Plastic / Glass H2SO4 pH <2 +0-6°C 200 mL 4500NH3 B Nitrogen, Total Organic (TON) TKN-NH3= TON calc 28 days Plastic / Glass H2SO4 pH <2 +0-6°C 200 mL 6 hours (recommended) / Glass only 0-6°C 250 mL Odor; SM 2150 B 24 hour regulatory Oil & Grease, Total and/or Hydrocarbons EPA 1664 28 days Glass only-in duplicate HCl orH2SO4 pH<2+0-6°C 1 L {10} Chlorinated Pesticides & PCBs, EPA 625.1 7 days, Aqueous Glass Amber Liter {6} 0-6°C {5} 1 L (formerly EPA 608) /8081/8082 14 days, Sludge or Solid Solids in Glass SJ 0-6°C 1 8oz
(Rev 03/02/2021) 1885 North Kelly Road • Napa, California 94558 (707) 258-4000 • Fax (707) 226-1001 • e-mail: info@caltestlabs.com NELAP/ORELAP Certification 4036 CA-ELAP Certification 1664 Maximum Holding Time Required Required Minimum Analytical Parameter Container Type Preservative Amount Polynuclear Aromatic Hydrocarbons 7 days, Aqueous Glass Amber Liter {6} Solids in 0-6°C {5} 1 L (PAH’s) EPA 625.1/8270 14 days, Sludge or Solid Glass SJ 0-6°C 1 8oz 7 days, Aqueous Glass Amber Liter. Collect In 0-6°C {5} 1 L Organophosphorus Pesticides triplicate 0-6°C 1 8oz EPA 625.1 (formerly EPA 614) /8141 Solids in Glass SJ 14 days, Sludge or Solid Semi-volatile Organics 7 days, Aqueous Glass Amber Liter {6} 0-6°C {5} 1 L EPA 625.1/8270 14 days, Sludge or Solid Solids in Glass SJ 0-6°C 1 8oz 7 days /3 days {9} Glass Amber Liter. Collect In 0-6°C {5} 1 L Pyrethroid Pesticides triplicate GCMS-NCI-SIM/ EPA 8270M/625.1M Solids-Amber Glass SJ 14 days, soil or sediment Solids to be Frozen 1 8oz 7 days, Aqueous Glass Amber Liter. Collect In 0-6°C 1 L Carbamate Pesticides 14 days, Sludge or Solid triplicate EPA 632/8321 Solids in Glass SJ Volatile Organics 14 days, preserved Glass VOA vial {1} (sample in HCl pH <2 + 0-6°C {4} 40 mL EPA 524.2 {4} triplicate) 14 days except: Total of four 40 mL VOA Vials; 0-6°C One 40mL of Volatile Organics 3 days unpreserved for Two Pres w/ HCl, Two (see holding time for each EPA 624.1 {3} Acrolein {12} Unpreserved {1} (Includes Acrolein and Acrylonitrile) additional preservation) container type 7 days unpreserved for BTEX only {11}. described THMS only—3 HCl VOAs 14 days (aqueous) Four (4) Glass VOA vial {1} HCl pH <2 + 0-6°C 40 mL Volatile Organics; EPA 8260 {3} 14 days Sludge/solid Solids in separate glass jar 0-6°C 1 8oz filled to top TPH Diesel/Motor Oil; EPA 8015 7 days Glass Amber Liter {6} 0-6°C 1 L Tributyltin (TBT) 7 days (recommended) Glass Amber Liter {6} 0-6°C 1 L TPH Gas/ BTEX/ MTBE; EPA 8260 14 days preserved Glass VOA vial {1} HCl pH <2 + 0-6°C 40 mL Dioxin; EPA 1613 1 year Glass Amber Liter {6} 0-6°C {5} 1 L Perchlorate; EPA 314.0 28 days Plastic/Glass 0-6°C 100 mL pH; SM 4500 H+ B 15 minutes Plastic / Glass 0-6°C 100 mL Phenols, EPA 420.1 28 days Glass Amber 250 mL H2SO4 pH <2 +0-6°C 200 mL Phosphate, Ortho (as P); SM 4500P E 48 hours Plastic / Glass 0-6°C 100 mL 15 minutes to filter / 48 Plastic / Glass 0-6°C 100 mL Phosphate, Ortho, Dissolved (as P); SM 4500P E hrs once filtered Phosphorus, Total (PO4 as P); SM 4500P B/F or B/E 28 days Plastic / Glass H2SO4 pH <2+0-6°C 100 mL Solids, Settleable (SS); SM 2540 F 48 hours Plastic / Glass 0-6°C 1000 mL Solids, Total (mg/L or %); SM 2540 B, or SM 2540 G 7 days Plastic / Glass 0-6°C 100 mL Solids, Total Dissolved (TDS); SM 2540 C 7 days Plastic / Glass 0-6°C 100 mL Solids, Total Suspended (TSS); SM 2540 D 7 days Plastic / Glass 0-6°C 200 mL Solids, Volatile Suspended (VSS); SM 2540 E 7 days Plastic / Glass 0-6°C 200 mL Sulfate (SO42-), EPA 300.0 28 days Plastic / Glass 0-6°C 100 mL Sulfide, Dissolved; SM 4500 S2- B/D 7 days Glass 250 mL AlCl3 + NaOH +0-6°C 250 mL 7 days Glass (preferred)/plastic (no NaOH + ZnAC pH >9 + 0- 250 mL Sulfide, Total; SM 4500 S2- C/D headspace) 6°C Sulfite (SO3) 7 days Glass Amber 500 mL EDTA + 0-6°C 250 mL Surfactants (MBAS); SM 5540 C 48 hours Plastic / Glass 0-6°C 250 mL Suspended Sediment Conc. (SSC); ASTM D3977 7 days Plastic / Glass 0-6°C {7} 100 mL Total Organic Carbon (TOC); SM 5310 B 28 days Amber Glass VOA (3 vials) HCl pH <2 + 0-6°C 40 mL Turbidity; SM 2130 B, or EPA 180.1 48 hours Plastic / Glass 0-6°C 100 mL Ultraviolet Absorption (UVA at 254nm); SM 5910 B 48 hours Glass Amber 125mL 0-6°C 125 mL
{1} Volatile organic samples need to be filled in multiple VOA vials without air bubble s/headspace (≤6 mm in size). {2} Dissolved metals require field or lab filtration through 0.45-micron filter prior to preservation. 40 CFR 136.3 requires filtration within 15 minutes. {3} Volatile organic methods EPA 624.1 and 8260 require dechlorination using Sodium Thiosulfate (Na2S2O3) at time of sampling if chlorine is present. (Thiosulfate dechlorination bottles are available at the laboratory upon request.) Dechlorination is to occur before transferring to the appropriate VOA. {4} Volatile organic method EPA 524.2 requires de-chlorination using ascorbic acid at time of sampling if chlorine is present. Ascorbic acid dechlorination kits are available at the laboratory upon request. If analyzing for THMs only, Sodium Thiosulfate (Na2S2O3) may be used, and acidification can be omitted. Otherwise, dechlorinate with Ascorbic acid, then preserve with HCl. If the sample foams vigorously upon addition of HCl, discard and collect unpreserved dechlorinated sample, and notify the laboratory as the samples must be analyzed within 24 hours of collection if they are to be analyzed for any compounds other than THMs. Method 524.2 requires a travel/trip blank with each sample set collected. {5} If sampling from a chlorinated location, add 80 mg/L Sodium Thiosulfate (Na2S2O3) per liter and mix well. Any method suitable for field use may be employed to test for residual chlorine (Reference 16). Add more sodium sulfate if 80 mg/L is insufficient but do not add excess sodium thiosulfate. {6} Semi Volatile Organics Amber Liters (AL) should be collected in duplicate, to ensure volume for re-extraction if necessary. When possible, please collect 4 AL per method to allow the laboratory to perform matrix Quality Control (MS/MSD). {7} Suspended Sediment Conc.(SSC) requires its own container and the entire contents are used for the analysis. {8} Preserve with HCl if less than 10 ppT Salinity, or Preserve with H2SO4 if greater than 10 ppT Salinity. {9} Cyhalothrin in water has a 3-day hold time in reagent water per USGS study. Permethrin in water has a 3-day hold time per Storage Stability Study by CA Dept. of Food and Agriculture. {10} 1000 mL required for RL of 5 mg/L; 250 mL bottle recommended for samples with expected concentrations of 20 mg/L or higher. {11} If unpreserved, must be analyzed within 7 days of sampling {12} Hold time can be extended to 14 days if preserved to pH 4-5 at time of collection.
