Introduction of K2025 HPLC
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Lani Liu
2/23/2023
Overview
01 About Wooking
02
Principles of HPLC
03
03 K2025HPLC
司
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01 About Wooking
Hanon Advanced Technology Group Company
Shandong Wooking Instrument was established in 2018 and is a subsidiary of Hanon
Advanced Technology Group Company.
Hanon is a scientific instrument service provider, specialized in the research and
development of scientific instruments and analytical methods, dedicated to providing
overall solutions for food, pharmaceuticals, agriculture, chemical industry, environmental
protection, medical diagnosis and other industries.
Brands
Hanon Sineo GAS Wooking
Kjeldahl Protein Analyzer No.1 market share in GC-IMS HPLC
Fiber Analyzer China (Microwave (gas chromatography to
Fat Analyzer digestion) ion mobility )
02 Principles of HPLC
What is HPLC?
high-performance liquid chromatography
• Separation of a mixture into individual components
• The separation uses a Column as stationary phase and Liquid as mobile phase
• Based on differences of affinity for the stationary phase and mobile phase
High Performance Liquid Chromatography \ HPLC
Mobile Phase/Solvent Column Oven Detector
Sample
Pump Injector Waste
Wooking K2025
03 K2025 HPLC
K2025 HPLC
þ Reliability
þ Precision
þ Easy-to-use
þ Compliance
K2025 HPLC
þ Reliability
þ Precision
þ Easy-to-use
þ Compliance
K2025 Reliability
The considerations of reliability are part of FDA's concerns in reference to their safety and
effectiveness.
The following considerations with respect to reliability are important in a manufacturing operation:
Design Produce Manage Test
Reliability Design Produce Manage Test
Pump Autosampler Oven UVD
Material：High hardness alloy steel Needle：Constant-pressure Leakage sensor Static optical path
Gearing：Self-lubricating mechanism Wash solvents：Degassing unit Monitoring Over-temperature
Power：High power customized motor Arm：Single track Over-temperature Fully enclosed structure
Plunger：Floating plunger pump：real-time compensation
Reliability Produce Manage Test
HAMAMATSU Systec Rheodyne HORIBA
SMC SGE Ceramaret PSI
Reliability Produce Manage Test
Whole industrial chain manufacturing mode
Machining Sheet metal
S u r f a c e M o u n t Te c h n o l o g y Spraying Injection
Reliability Manage Test
Reliability Test
Endurance test
Cam：10 years
Plunger：150 L
Injection：300000 times
Reliability Test
K2025 HPLC
þ Reliability
þ Precision
þ Easy-to-use
þ Compliance
K2025 Pump-Precise flow rate
Stable and precise flow rate!
Without pressure suppression
Tandem pump
With pressure suppression
Pressure dynamic suppression algorithm
Mixer
Pressure fluctuation test
K2025 Pump-Precise gradient ratio
Gradient
RSD%（n=11）
（B%）
2% 0.040
5% 0.028
10% 0.038
30% 0.023
50% 0.016 Gradient test
50.1% 0.014
70% 0.007
90% 0.007
95% 0.002
98% 0.019
斜线梯度 0.260
R.T. RSD(%)
No. Sample
n=11
1 Caffeine 0.033
2 Methylparaben 0.023
3 Ethylparaben 0.030 Reproducible results!
4 Diethyl phthalate 0.025
≤0.2
K2025 Autosampler-Precise injection volume
integrated and
constant-pressure Needle
Repeatability test of 1000-time continuous injection and analysis
Caffeine Peak Area[mAU.s]
Caffeine R.T.[min]
8 1000
7.8
7.6 900
峰面积/[mAU.s]
保留时间/min
7.4 800
7.2
7 700
6.8
6.6 600
6.4 500
6.2
6
Precise Injection 0 200 400 600 800 1000
400
0 200 400 600 800 1000
pump 进样次数/n 进样次数/n
Conclusion: The RSD of Caffeine retention time is 0.106%，The RSD of Caffeine
peak area is 0.449%.
K2025 Autosampler-Low carr yover
Highly polished for exterior surface and passivated for interior surface→reduce sample residue
Pre-injection and post-injection needle wash can be performed
carryover=peak area of the blank sample after a certain concentration sample/peak area of certain concentration sample
Sample Area Carryover
Caffeine
3000ppm Overload
Blank after
needle wash 0.559
Caffeine
150ppm 3286.287
K2025 Column Oven-Precise temperature control
Peltier element+fan-based forced air→more stable and constant
K2025 UVD-High sensitivity
Low baseline noise and drift results in low detection limits
3�� × � 3 × 0.005 × 1.0 × 10 − 7
��� ���� = = = 2.4 × 10 − 9 g/��
� 0.609
Naphthalene
K2025 UVD-Wide linear range
Wide linear range (>2.5 AU) for reliable, simultaneous quantification of primary
compounds, by-products and impurities.
3500
5.47%
3000
1.35%
1.58%
2500 2.54%
1.94%
1.83%
Intensity/mAU
y = 185.133x - 1.5528
2000 R² = 0.9999 2.42%
2.04%
1500 1.30%
1.35%
1.17%
1000 0.82%
500
0
0.00% 0.20% 0.40% 0.60% 0.80% 1.00% 1.20% 1.40% 1.60% 1.80% 2.00%
Ace. Conc.
K2025 DAD-High sensitivity and resolution mode
Similar sensitivity to UVD High spectral resolution
Benzene
1nm/2nm/4nm/8nm bandwidth available
K2025 DAD-Precision
DAD provides full-wavelength information. The compounds can be identified by similarity
comparison in the spectral database. Peak purity determination can be performed in the workstation.
K2025 Wookinglab-powerful data processing capability
Up to 27 integration events and 3 quantitative calculation methods (external standard method,
internal standard method, and normalization method) for complicated data
31
K2025 HPLC
þ Reliability
þ Precision
þ Easy-to-use
þ Compliance
K2025 Pump-Easy to Use
Ø Plunger rinsing Ø Purge buttons
Customer benefits: Customer benefits:
• Prevent precipitation of buffer solution • Stop in 5 mins automatically
• Periodically, without additional settings • No need to purge in software
K2025 Pump- Easy to Use
Ø Selection valve(Binary pump) Ø Independent 4-channel gradient valve(Quaternary pump)
Degasser Degasser
Purge valve
A1 A2 Sensor Purge valve B1 B2
Pump
Gradient
valve
Mixer
Mixer
Customer benefits: Customer benefits:
• Easy switching of solvents • Easy maintenance
• Not optional kit • Real-time monitoring
K2025 Autosampler-Easy to Use
• Overlapped injection mode: injection cycle time as low as 5s
Injecting Running Injecting Running
Injecting Running Injecting(Overlapped) Running
K2025 Autosampler-Easy to Use
• Easy maintenance
Needle wash solvent In-front valve
Self-reset tray
K2025 Column Oven-Protection
Triple protection for safe use
3：
Over-temperature
2： protection
Real-time monitoring
1：
Liquid leakage
K2025 UVD-Easy to use
Stop-flow wavelength scanning Wavelength time programming
A full wavelength scanning mode can be Wavelength can be set for different analytes
performed to easily find the optimal absorption during different periods, to accomplish the
wavelength of the target analyte. high-sensitivity analysis of complex samples
K2025 DAD-Easy to Use
12 channel real-time monitoring
Up to 12 wavelengths can be set for real-time
detection;·Sampling rate up to 125 Hz
K2025 Wookinglab-Easy to Use
The workstation interface is clear, allowing users to quickly complete method development, data
acquisition, data processing, report editing and printing, etc.
Organized
navigation
Convenient operation
Clear status
monitoring
40
K2025 HPLC
þ Reliability
þ Precision
þ Easy-to-use
þ Compliance
K2025 Wookinglab-Compliance
Database storage
With the professional database system, the encryption of original files and
security of raw data can be guaranteed
Backup and restore
Backup and restore of data can be easily performed in the workstation,
which is convenient for users
Multi-level user system
Supports customized multi-level system for access to data, ensuring the
effectiveness of the system
Traceability
The workstation complies with FDA 21 CFR Part 11, with complete records
and complete traceability.
42
Wooking K2025 Reliability Precision Easy-to-use Compliance
Features and benefits
l 0.001-10.000mL/min settable, 0.001
l 108 vials(2mL)（96/384 well-plate optional）
increments
l Low carryover（0.003%）
l Max. Pressure 62MPa，UHPLC
l Injection time shorten to 5s(overlapped mode)
l Minimum pressure pulsation(<10psi)
l Sample temp. control(4-40℃) optional
l Flow accuracy 0.5%
l Needle wash(inner and outer surface)
l Built-in degasser, integrated design
l In-front design
l Solvent switching valve(P2)
l Independent 4-channel valve(P4)
l Light source: D2 and W
l Wavelength calibration
l Peltier-element and fan-based force air； l Spectral scanning(UVD)
l Temp. control: ambient-10℃ to 85℃； l Wavelength time programming(UVD)
l Temp. stability ±0.1℃(typically 0.05℃) l Peak purity calculation(DAD)
l 12-Channel real-time monitoring(DAD)
l 100Hz acquisition rate
THANK YOU!
