Name:______________________ _______________________Period:_______
DNA Replication Practice
Directions: Below are the 3 steps in DNA replication. Follow the directions for each step and then
answer the questions below.
1. -What is happening to the DNA molecule in the figure?
(Explain the first step in DNA replication)
The two strands of the double helix separate, or "unzip",
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allowing two replication forks to form. (Helicase)
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___________________________________________________
2. -What happens to the DNA molecule during the second
step of DNA replication?
Enzymes active = helicose + gyrase
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Helicose - breaks H-bonds between
complementary pairs
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Gyrase - releases tension in the DNA
strand as it unwinds
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3. -What happens during the third step of DNA replication?
Termination
During termination, DNA
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replication comes to an end.
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during that enzyme removes the DNA primers and
replaces them with DNA
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How DNA Is Copied
a with t and c with g
4. What does it mean that the two strands of DNA are complementary? ______________
the ______________________________________________________________________
sequence of bases in one strand can be used to create the correct sequence of bases in the other strand
5. it is process by which DNA makes a copy of itself during cell division./ duplicating
What is DNA replication?________________________________________________
6. Using your notes, book, and this assignment, place the steps of DNA replication in the correct order.
____
2 a. The enzyme DNA polymerase moves along the exposed strands and adds
complementary nucleotides to each nucleotide in each existing strand.
1
____ b. The DNA double helix breaks or unzips down the middle between the base pairs.
____
3 c. A complementary strand is created for each of the two strands of the original
double helix.
4
____ d. Two new identical DNA molecules have been produced.
7. (True or False) The process of DNA replication results in a copy of the original DNA molecule.
8. (True or False) DNA does not have to break apart to be copied.
9. (True or False) After DNA replication is complete, there are two new DNA molecules; one molecule
has both of the original strands and one molecule has two new strands of DNA.
in the nucleus in
10. Where does DNA replication happen?________________________________________
eukaryotes, whereas in
______________________________________________________________________.
cytoplasm in prokaryotes.
during the Synthesis (S) synthesis phase of interpahse
11. When does DNA replication happen?________________________________________
phase of cell cycle (before mitosis starts
12. Below are DNA strands. Make the complementary DNA strand:
Original Strand: A T G C A A A T T G C T C A C C G G G G A T C A G C A C C G G
Complementary Strand: _____________________________________________________
TACGTTTAACGAGGCCCCTAGACGTGGCC
Original Strand: A G G G G A T C A G C A C C G G A T T T C A T G A G C C C T A
Complementary Strand: ____________________________________________________
TCCCCTAGTCGTGGCCTAAAGTACTCGGGAT
Original Strand: A A G T A C G A T C G A T G C A C A T G C A T G G C T A C G C
Complementary Strand: ______________________________________________________
TTCATGCTGCTACGTGTACGTACCGATGCG
by helicase
by DNA polymerase
by DNA polymerase and then DNA winds up.
