SDLX-IM-8

Spinchron™ DLX Benchtop Centrifuge

Instruction Manual

SPEED

TIME

ACC/DEC

RPM
RCF
ROTOR
TIME

ACCEL ENTER
IMBALAN
CE OPEN
DOOR DECEL
PULSE
FAST
STOP
MADE ® STOP
START
IN U.S.A.

™
Symbol Simbolo
Symbol
Title / Titel / Titre / Titulo / Titolo / /
Symbole
Símbolo

Dangerous voltage
Gefährliche elektrische Spannung
Courant haute tension
Voltaje peligroso
Pericolo: alta tensione

Attention, consult accompanying documents

Achtung! Begleitpapiere beachten!
Attention, consulter les documents joints

!
Atención, consulte los documentos adjuntos
Attenzione: consultare le informazioni allegate

On (power)
Ein (Netzverbindung)
Marche (mise sous tension)
Encendido
Acceso (sotto tensione)

Off (power)
Aus (Netzverbindung)
Arrêt (mise hors tension)
Apagado
Spento (fuori tensione)

Protective earth (ground)

Schutzleiteranschluß
Liaison à la terre
Puesta a tierra de protección
Collegamento di protezione a terra

Earth (ground)
Erde
Terre
Tierra
Scarica a terra
 ! SAFETY NOTICE
This safety notice summarizes information basic to the safe operation of the equipment described
in this manual. The international symbol displayed above is a reminder that all safety instructions
should be read and understood before installation, operation, maintenance, or repair of this centri-
fuge. When you see the symbol on other pages, pay special attention to the safety information
presented. Observance of safety precautions will also help to avoid actions that could damage or
adversely affect the performance of the centrifuge.

Safety During Installation and/or Maintenance

This centrifuge weighs 65.4 kg (144.0 lb). DO NOT attempt to lift or move the centrifuge without
assistance from another person.

Be sure to use the anchoring system to secure the centrifuge in place. The anchoring system is
designed to reduce the possibility of injury or damage which could result from centrifuge move-
ment in the event of a major rotor mishap.

Any servicing of this centrifuge that requires removal of any covers can expose parts which involve
the risk of electric shock or personal injury. Make sure that the power switch is off and the centri-
fuge is disconnected from the main power source, and refer such servicing to qualified personnel.

Do not replace any centrifuge components with parts not specified for use on this instrument.

Electrical Safety
To reduce the risk of electrical shock, this centrifuge uses a three-wire electrical cord and plug to
connect this equipment to earth-ground. To preserve this safety feature:

• Make sure that the matching wall outlet receptacle is properly wired and earth-grounded. Check
that the line voltage agrees with the voltage listed on the name-rating plate affixed to the
centrifuge.

• Never use a three-to-two wire plug adapter.

• Never use a two-wire extension cord or a two-wire non-grounding type of multiple-outlet

receptacle strip.

Do not place containers holding liquid on or near the chamber door. If they spill, liquid may get into
the centrifuge and damage electrical or mechanical components.

Safety Against Risk of Fire

Certain electrical circuits within this centrifuge are protected by fuses against overcurrent condi-
tions. For continued protection against the risk of fire, replace only with the same type and rating
specified.

This centrifuge is not designed for use with materials capable of developing flammable or explo-
sive vapors. Do not centrifuge such materials (such as chloroform or ethyl alcohol) in this centri-
fuge nor handle or store them within the 30-cm (1-ft) area surrounding the centrifuge.
Mechanical Safety
For safe operation of the centrifuge, observe the following:

• Use only the rotors and accessories designed for use in this centrifuge.

• Before starting the centrifuge, make sure that the rotor tie-down nut is securely fastened.

• Do not substitute a metal fastener for the plastic tie-down nut supplied with the rotor. If a
tie-down nut comes off the shaft during centrifugation, the plastic nut furnished will break apart
in the chamber, causing minimal damage to the centrifuge. A loose metal object could substan-
tially damage the rotor, chamber, and lid, and could potentially escape the chamber into the
laboratory, causing personal injury or property damage.

• Do not exceed the maximum rated speed of the rotor in use.

• NEVER attempt to slow or stop the rotor by hand.

• Do not lift or move the centrifuge while the rotor is spinning.

• If a glass tube breaks inside the chamber bowl, be careful when examining or cleaning the
gasket or chamber, as sharp glass fragments may be embedded in their surfaces.

• NEVER attempt to override the door interlock system while the rotor is spinning.

• Maintain a 7.6-cm (3-in.) clearance envelope around the centrifuge while it is running. During
operation do not come within the envelope except to adjust centrifuge controls, if necessary.
Never bring any flammable substances within the 30-cm (1-ft) area surrounding the centrifuge.
Never lean on the centrifuge or place items on the centrifuge while it is operating.

Chemical and Biological Safety

Normal operation may involve the use of solutions and test samples that are pathogenic, toxic, or
radioactive. Such materials should not be used in this centrifuge, however, unless all necessary
safety precautions are taken.

• Observe all cautionary information printed on the original solution containers prior to their use.

• Handle body fluids with care because they can transmit disease. No known test offers complete
assurance that they are free of micro-organisms. Some of the most virulent—Hepatitis (B and
C) and HIV (I–V) viruses, atypical mycobacteria, and certain systemic fungi—further empha-
size the need for aerosol protection.

• Handle other infectious samples according to good laboratory procedures and methods to
prevent spread of disease.

• Because spills may generate aerosols, observe proper safety precautions for aerosol contain-
ment. Use only the appropriate rotors and adapters. Do not run toxic, pathogenic, or
radioactive materials in this centrifuge without taking appropriate safety precautions. Biosafe
containment should be used when Risk Group II materials (as identified in the World Health
Organization Laboratory Biosafety Manual) are handled; materials of a higher group require
more than one level of protection.

• Dispose of all waste solutions according to appropriate environmental health and safety
guidelines.

It is your responsibility to decontaminate the centrifuge and accessories before requesting

service by Beckman Coulter Field Service.
 SDLX-IM-8
December 2007

Spinchron™ DLX Benchtop Centrifuge

Instruction Manual

SPEED

TIME

ACC/DEC

RPM
RCF
ROTOR
TIME

ACCEL ENTER
IMBALAN
CE OPEN
DOOR DECEL
PULSE
FAST
STOP
MADE ® STOP
START
IN U.S.A.

© 2007–2009 Beckman Coulter, Inc.

 Contents

Page

INTRODUCTION
Certification . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . vii
Scope of Manual. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . vii
Conventions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . viii
CFC-Free Centrifugation. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ix
Radio Interference . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . x
Recycling Label . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . x

SECTION 1: DESCRIPTION

Centrifuge Function, Specifications, and Safety Features . . . . . . . . . . 1-1

Centrifuge Function . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-1
Specifications. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-2
Safety Features. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-2
Centrifuge Chassis . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-3
Housing . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-3
Door . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-3
Rotor Chamber. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-4
Drive . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-4
Control Panel . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-5
System Keys . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-5
Parameter Keys . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-6
Digital Displays . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-7
Available Rotors. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-9

SECTION 2: INSTALLATION REQUIREMENTS

Installation Requirements. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-1

Space and Location Requirements. . . . . . . . . . . . . . . . . . . . . . . . . 2-2
Electrical Requirements. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-3
Test Run. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-4
Name Rating Plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-4

iii
Contents

Page

SECTION 3: OPERATION

Run Preparation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-2

Setting Run Parameters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-3
Selecting a Rotor . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-4
Setting Run Speed . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-4
Setting Run Time. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-5
Setting Acceleration and Deceleration Rates . . . . . . . . . . . . . . . . 3-6
Starting a Run. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-7
Changing Parameters During a Run. . . . . . . . . . . . . . . . . . . . . . . . . . . 3-8
Stopping a Run . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-9
Unloading . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-10
Summary of Run Procedures . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-11

SECTION 4: TROUBLESHOOTING

User Messages . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-1

Other Possible Problems . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-3
Accessing the Rotor in Case of Power Failure. . . . . . . . . . . . . . . . . . . 4-4

SECTION 5: CARE AND MAINTENANCE

General Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-1

Cleaning . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-2
Decontamination . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-3
Sterilization and Disinfection. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-3
Tube Breakage . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-3
Storage and Transport . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-4
Storage . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-4
Returning a Centrifuge . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-4
Supply List . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-5
Replacement Parts . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-5
Supplies . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-5

Warranty

iv
 Contents

Illustrations

Page

Figure 1-1. Interior View of the Spinchron DLX Centrifuge Rotor

Chamber . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-4

Figure 2-1. Dimensions of the Spinchron DLX Centrifuge . . . . . . . . . . . . . . . . . . 2-2

Tables

Page

Table 3-1. Acceleration and Deceleration Rates (in Minutes:Seconds)

to and from Maximum Speed. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-6

Table 4-1. Error Message Chart. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-2

Table 4-2. Troubleshooting Chart . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-3

v
 Introduction

CERTIFICATION
To ensure full system quality, Beckman Coulter Spinchron™ DLX
centrifuges are manufactured in a registered ISO 9001 or 13485
facility. They have been designed and tested to be compliant (when
used with Beckman Coulter rotors) with the laboratory equipment
requirements of applicable regulatory agencies. Declarations of
conformity and certificates of compliance are available at
www.beckmancoulter.com.

International symbols that may be displayed on the centrifuge are

illustrated and described on the inside front cover of this manual.

SCOPE OF MANUAL
This manual is designed to familiarize you with the Beckman Coulter
Spinchron DLX centrifuge, its functions, specifications, operation,
and routine operator care and maintenance. We recommend that you
read this entire manual, especially the SAFETY NOTICE and all
safety-related information, before operating the centrifuge or
performing instrument maintenance.

➠ NOTE
A magnetized document holder (964987) is
available, which can be attached to either side
of the centrifuge for convenient storage of this
manual, as well as applicable rotor manuals.
The rotor tie-down torquing bar (356036) and
emergency latch release hex wrench (929317)
can also be kept in the document holder for
easy access.

vii
Introduction

• Section 1 contains system specifications and a brief physical and

functional description of the centrifuge, including the operating
controls and indicators.

• Section 2 provides instructions for installing and connecting the

centrifuge.

• Section 3 contains centrifuge operating procedures. For experi-

enced users, a summary of operating procedures is provided at the
end of the section.

• Section 4 lists possible error messages and/or malfunctions,

together with probable causes and required corrective actions.

• Section 5 contains procedures for routine operator care and main-

tenance, as well as a brief list of supplies and replacement parts.

• A Program Library chart is provided as an appendix at the back of

this manual. You can use this chart to record the parameters of a
run for later duplication of the run conditions.

If the centrifuge is used in a manner other than specified in this

manual, the safety and performance of this equipment could be
impaired. Further, the use of any equipment other than that recom-
mended by Beckman Coulter has not been evaluated for safety. Use
of any equipment not specifically recommended in this manual and/or
the appropriate rotor manual is the sole responsibility of the user.

CONVENTIONS
Certain symbols are used in this manual to call out safety-related and
other important information. These are reproduced and described
below and on the inside of the front cover.

NOTES, CAUTIONS, AND WARNINGS

➠ NOTE
Used to call attention to important information
that should be followed during installation, use,
or servicing of this equipment.

viii
 Introduction

! CAUTION
Used to indicate a potentially hazardous situa-
tion which, if not avoided, may result in minor or
moderate injury and/or mechanical damage. It
is also used to alert against unsafe practices.

! WARNING
Used whenever an action or condition may
potentially cause personal injury or loss of
life. Mechanical damage may also result.

WARNING
Indicates high voltage or risk of electric
shock. Refer servicing of all areas displaying
either symbol to service personnel.

TYPOGRAPHIC CONVENTIONS

Certain typographic conventions are used throughout this manual to

distinguish names of user interface components, such as keys and
displays.

• Key names (for example, [START] or [ENTER]) appear in brackets.

• Display names (for example, TIME or SPEED) appear in bold

type.

• Cursor keys, used to increment values up or down when setting

parameters, are shown as up and down arrows (▲ and ▼).

CFC-FREE CENTRIFUGATION
To ensure minimal environmental impact, no CFCs are used in the
CFC manufacture or operation of Spinchron DLX centrifuges.

ix
Introduction

RADIO INTERFERENCE
This equipment has been tested and found to comply with the limits
for a Class A digital device, pursuant to Part 15 of FCC Rules. These
limits are designed to provide reasonable protection against harmful
interference when the equipment is operated in a commercial environ-
ment. This equipment generates, uses, and can radiate radio
frequency energy and, if not installed and used in accordance with
this instruction manual, may cause interference to radio communica-
tions. Operation of this equipment in a residential area is likely to
cause interference, in which case the user will be required to correct
the interference at his own expense.

CANADIAN REGULATIONS

This equipment does not exceed the Class A limits for radio noise
emissions from digital apparatus as set out in the radio interference
regulations of the Canadian Department of Communications.

Le présent appareil numérique n’émet pas de bruits radioélectriques

dépassant les limites applicables aux appareils numériques de Classe
A prescrites dans le reglement sur le brouillage radioelectrique édicté
par le Ministère des Communications du Canada.

RECYCLING LABEL
This symbol is required in accordance with the Waste Electrical and
Electronic Equipment (WEEE) Directive of the European Union. The
presence of this marking on the product indicates:

A28219-AA 1) the device was put on the European market after August 13, 2005
and
Note: On the instrument, the
triangle background is yellow 2) the device is not to be disposed via the municipal waste collection
rather than gray.
system of any member state of the European Union.

It is very important that customers understand and follow all laws

regarding the proper decontamination and safe disposal of electrical
equipment. For Beckman Coulter products bearing this label please
contact your dealer or local Beckman Coulter office for details on the
take back program that will facilitate the proper collection, treatment,
recovery, recycling and safe disposal of the device.

x
 1
Description

CENTRIFUGE FUNCTION, SPECIFICATIONS, AND SAFETY FEATURES

CENTRIFUGE FUNCTION

The Beckman Coulter Spinchron DLX is a nonrefrigerated compact

benchtop centrifuge that generates centrifugal forces required for a
wide variety of applications. Together with the Beckman Coulter
rotors designed for use in this centrifuge, the centrifuge applications
include:

• Routine processing such as sample preparations, pelleting, extrac-

tions, purifications, concentrations, phase separations, receptor
binding, and column centrifugations.

• Processing large numbers of small-volume samples in multiwell

plates for concentrating tissue-culture cells, cloning and replicate
studies, in-vitro cytotoxicity studies, receptor binding, and genetic
engineering experimentation.

• Rapid sedimentation of protein precipitates, large particles, and

cell debris.

• Binding studies and separation of whole blood.

• Cell isolation.

The centrifuge is microprocessor-controlled, providing interactive

operation. The centrifuge design features a brushless induction drive
system, program memory that can repeat the previously used run
parameters, and a choice of acceleration and deceleration rates. User
messages alert the operator to conditions that may need attention.

1-1
Description

SPECIFICATIONS

Only values with tolerances or limits are guaranteed data. Values

without tolerances are informative data, without guarantee.

Speed
Setting range . . . . . . . . . . . . . 0 to 3750 rpm (in 100-rpm increments)
or equivalent RCF
Time
Setting range . . . . . . . . . . . . . . . . . . 1 min to 9 hr 59 min (timed run),
or continuous,
or PULSE
Display (timed run) . . . . . . . . . . . . . . . . . . . . . . . . run time remaining
Display (continuous run) . . . . . . . . . . . . . . ∞ symbol and elapsed time
Acceleration . . . . . . . . . . . . . . . . . . . . . . . . . . . . nine acceleration profiles
Deceleration . . . . . . . . . . . . . . . . . . . . . . . . . . . . nine deceleration profiles
Dimensions
Width . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 54.6 cm (21.5 in.)
Depth, overall. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 66.0 cm (26.0 in.)
Depth at base . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 58.4 cm (23.0 in.)
Height, door closed . . . . . . . . . . . . . . . . . . . . . . . . . 40.6 cm (16.0 in.)
Height, door open. . . . . . . . . . . . . . . . . . . . . . . . . . . 96.8 cm (38.1 in.)
Weight . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 65.4 kg (144.0 lb)
Surface finish
Instrument housing. . . . . . . . . . . . . . . . . . . . . . . . . . . . . urethane paint
Control panel overlay. . . . . . . . . . . . . . . . . . . . . coated polycarbonate
Electrical supply . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Class I
Clearances required (sides and rear) . . . . . . . . . . . . . . . . . . 7.6 cm (3.0 in.)
Maximum heat dissipation into room
under steady-state conditions . . . . . . . . . . . . . . . 0.9 kW (3701 Btu/hr)
Ambient temperature range . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10 to 35°C
Humidity limitation . . . . . . . . . . . . . . . . . . . . . . . . . <95% (noncondensing)
Noise level 1 m (3.3 ft) in front of centrifuge . . . . . . . . . . . . . . . . . ≤62 dBa
Installation (overvoltage) category . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . II
Pollution degree. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2*
_______________
* Normally only nonconductive pollution occurs; occasionally, however, a temporary
conductivity caused by condensation must be expected.

SAFETY FEATURES

The Spinchron DLX centrifuge has been designed and tested to

operate safely indoors at altitudes up to 2 000 m (6 562 ft). Safety
features include the following:

• The door has an electromechanical door-locking mechanism and a

secondary (manual) lock to prevent operator contact with spinning

1-2
 Description

rotors. When the door is closed it locks automatically. It can be

unlocked only by pressing the [OPEN DOOR] key, and opened only
when the power is on, the rotor is at rest, and the manual lock is in
the unlocked position. Two independent monitoring systems
prevent the door from opening if the rotor is spinning.

• A steel barrier ring surrounds the aluminum rotor chamber to

provide full operator protection.

• An imbalance detector monitors the rotor during the run, causing

automatic shutdown if rotor loads are severely out of balance. At
low speeds, an incorrectly loaded rotor can cause imbalance. Rotor
instability can also occur if the centrifuge is moved while running,
or if it is not resting on a level surface.

• An anchoring system is provided to secure the centrifuge in place.

The anchoring system is designed to minimize the possibility of
injury or damage that could result from centrifuge movement in
the event of a major rotor mishap.

CENTRIFUGE CHASSIS
HOUSING

The centrifuge housing is made of sheet steel over a steel chassis and
is finished with urethane paint. The control panel overlay is made of
coated polycarbonate. Polyvinyl chloride lines the interior walls of
the centrifuge housing for sound and temperature insulation.

DOOR

The structural steel door has two filtered air-intake ports for rotor
cooling. An electromechanical door lock system, together with a
manual lock, prevents run initiation unless the door is closed and
latched. When a run is in progress, the door locks automatically and
can be opened only when the power is on, the rotor is virtually
stopped (spinning less than 40 rpm), and the lock is in the unlocked
position. (A light-emitting diode [LED] on the [OPEN DOOR] key
lights up when the door can be opened.) In the event of a power
failure, the door lock can be manually tripped for sample recovery
(see Section 4, TROUBLESHOOTING).

1-3
Description

ROTOR CHAMBER

The aluminum rotor chamber (Figure 1-1) is coated with a chemical-

resistant epoxy finish. The drive shaft (1) and neoprene rubber boot
(2) surrounding the drive shaft are visible in the chamber bottom.
A neoprene gasket (3) around the chamber top assures sealing.
(Centrifuge gaskets have not been designed as bioseals for aerosol
containment.)

During centrifugation, the rotor movement draws air through the

filtered air-intake ports in the door into the chamber bowl, where it
circulates around the rotor. Holes in the side of the bowl (4) allow air
to flow out to the exhaust ports beneath the centrifuge. This air flow
provides cooling for the rotor.

4 3

1 2

Figure 1-1. Interior View of the Spinchron DLX Centrifuge Rotor Chamber

DRIVE

The asynchronous three-phase direct-drive motor is brushless for

clean, quiet operation. A tie-down nut is used to attach the rotor to the
drive shaft. The resilient suspension ensures that loads will not be
disturbed by vibration, and prevents damage to the drive shaft if an
imbalance occurs during centrifugation. Maximum braking may be

1-4
 Description

selected to reduce deceleration time, allowing fast processing of

samples; alternately, delicate gradients may be preserved using
slower deceleration.

CONTROL PANEL
Mounted at an angle on the centrifuge front for easy visibility and
access, the control panel comprises touch keys—system keys and
parameter keys—and digital displays. An IMBALANCE LED at
the lower left of the panel flashes if the rotor load is severely out of
balance.

The power switch, a two-position rocker switch (off—O; on—I), is

mounted on the right side of the centrifuge.

SYSTEM KEYS

Centrifuge operation is controlled through the system keys. Each

system key (except [PULSE]) has an LED in the upper left corner that
lights up to indicate that the key can be activated.

SPEED TIME ACC/DEC

RPM RCF ROTOR TIME ACCEL ENTER

DECEL PULSE

OPEN FAST
IMBALANCE DOOR STOP STOP START

[OPEN DOOR] Unlatches the electromechanical lock and allows the door to be
opened (when the manual lock is in the unlocked position); the
centrifuge will accept this command only when the rotor is
completely stopped and the LED is lit.

[FAST STOP] Causes the centrifuge to decelerate to a complete stop at the

maximum rate; deceleration cannot be interrupted—the centrifuge
can only be restarted after the rotor stops and the door is opened
and closed.

1-5
Description

[STOP] When pressed, the centrifuge decelerates to a complete stop

according to the selected deceleration rate, deceleration can be
terminated and the centrifuge restarted by pressing [START] again.

[START] Causes a timed or continuous run to begin; can also be used to abort
deceleration and restart the centrifuge.

[PULSE] Causes the rotor to accelerate to set speed for short-duration runs (as
long as the key is pressed). Deceleration, at maximum rate, begins
when the key is released.

PARAMETER KEYS

Parameter keys are used to set run parameters (speed, rotor, time,
acceleration, and deceleration rate). Each parameter key (except for
the cursor keys) has an LED in the upper left corner that lights up to
indicate operational readiness. The LEDs also blink if an incorrect
parameter is entered.

SPEED TIME ACC/DEC

RPM RCF ROTOR TIME ACCEL ENTER

DECEL PULSE

OPEN FAST
IMBALANCE DOOR STOP STOP START

[RPM] Causes the last digit in the SPEED display (0) to flash, indicating that
speed can be entered in revolutions per minute (rpm).

[RCF] Selects speed setting by RCF; the corresponding rpm is automatically

calculated and displayed during the run. If this key is pressed during
the run, the SPEED display shows the RCF value.

[ROTOR] When this key is pressed the number of the rotor used in the previous
run is shown on the SPEED display. The list can be scrolled through,
using the cursor keys, to select a rotor.

1-6
 Description

[TIME] Selects the run duration.

• Timed run — can be set up to 9 hours and 59 minutes; a minutes

parameter exceeding 59 is automatically converted into hours.
• Continuous run — continuous operation is activated if a run time
of less than 0 or more than 9 hours and 59 minutes is selected (by
pressing the cursor key after the display reaches 0 or 9:59).

[ACCEL] Enables entering one of nine preset acceleration rates, using the
cursor keys. Rate selections are described in Table 3-1 (in Section 3).

[DECEL] Enables entering one of nine preset deceleration rates, using the
cursor keys. Rate selections are described in Table 3-1 (in Section 3).

▲ and ▼ (Cursor Keys) Pressed to increment parameter values up or down.

[ENTER] Pressed when all parameters are entered to enable [START]. Also press
to verify parameter changes (speed, time, temperature, and decelera-
tion rate) made while a run is in progress.

DIGITAL DISPLAYS

When centrifuge power is turned on, digital displays show the

operating parameters of the previous run. During operation,
the displays serve a dual purpose.

• When run parameters are being set (input mode), displays show
the set values (those selected by the operator). When a parameter
key (such as [TIME] or [RPM]) is pressed, the appropriate display
flashes to indicate that data can be entered.

• The actual (real-time) operating conditions of the centrifuge are

displayed during the run, after [START] is pressed.

Error messages (see Section 4) also appear in the displays, when

applicable.

SPEED TIME ACC/DEC

RPM RCF ROTOR TIME ACCEL ENTER

DECEL PULSE

OPEN FAST
IMBALANCE DOOR STOP STOP START

1-7
Description

SPEED The contents of this display vary, depending on the operating mode.

• In input mode, displays the value of the parameter being set

([RPM], [RCF], or [ROTOR]). For example, if the [ROTOR] parameter
key is pressed, a rotor number appears on the SPEED display.

• During centrifugation, displays the speed of the rotor in rpm; if the

[RCF] key is pressed during operation, the RCF value is displayed.

TIME The contents of this display vary, depending on the time mode
selected.

• Timed run (between 1 minute and 9 hours, 59 minutes) — begins

counting down when the rotor starts spinning and continues the
countdown until deceleration begins. Displays the remaining run
time in hours and minutes.

• Continuous run — countdown time is not displayed. The infinity

(∞) symbol, indicating continuous operation, lights up and the
display shows time elapsed since the run began. After 9 hours
and 59 minutes the timer resets to 0 and continues counting
elapsed time.

ACC/DEC Displays selected deceleration rate.

1-8
 Description

AVAILABLE ROTORS
Refer to the applicable rotor manuals for complete rotor descriptions.

Max
Max RCF Max Rotor Manual
Rotor Profile and Description RPM (× g) Capacity Number

GH-3.8A Swinging Bucket (4 place) GS6-TB-017

Buckets (rmax = 204 mm) 3750 3210 4 × 750 mL

Carriers (rmax = 163 mm) 3250 1924 4 × 192 mL

GH-3.8 Swinging Bucket (4 place) GS6-TB-003

Buckets (rmax = 204 mm) 3750 3210 4 × 750 mL

Carriers (rmax = 163 mm) 3250 1924 4 × 192 mL

PTS-DLXA Swinging Bucket (4 place) SP-TB-017

Rack base, 100-mm tube 3250 2050 16 × 10 mL

(rmax = 173 mm) (each carrier)

Rack base, 75-mm tube 3250 1800 16 × 7 mL

(rmax = 152 mm) (each carrier)

PTS-DLX Swinging Bucket (4 place) SP-TB-016

Rack base, 100-mm tube 3250 2050 16 × 10 mL
(rmax = 173 mm) (each carrier)

Rack base, 75-mm tube 3250 1800 16 × 7 mL

(rmax = 152 mm) (each carrier)

Sector base, 100-mm tube 3250 2010 7 × 10 mL

(rmax = 170 mm) (each carrier)

Sector base, 75-mm tube 3250 1890 7 × 7 mL

(rmax = 160 mm) (each carrier)

Tube base, 16-mm tube 3250 2080 20 × 10 mL

(rmax = 176 mm) (each carrier)

Tube base, 13-mm tube 3250 2080 20 × 7 mL

(rmax = 176 mm) (each carrier)

Micro Plus carriers 3250 1920 4 × 192 mL

(rmax = 163 mm)

Continued —

1-9
Description

Max
Max RCF Max Rotor Manual
Rotor Profile and Description RPM (× g) Capacity Number

PTS-2000 Horizontal (4 place) SP-TB-008

Sector base, 100-mm tube 3250 2010 7 × 10 mL
(rmax = 170 mm) (each carrier)

Sector base, 75-mm tube 3250 1890 7 × 7 mL

(rmax = 160 mm) (each carrier)

Tube base, 16-mm tube 3250 2080 20 × 10 mL

(rmax = 176 mm) (each carrier)

Tube base, 13-mm tube 3250 2080 20 × 7 mL

(rmax = 176 mm) (each carrier)

Micro Plus carriers 3250 1920 4 × 192 mL

(rmax = 163 mm)

GA-10 Fixed Angle, 35° (10 place)* GS6-TB-009

rmax = 123 mm 3750 1960 10 × 50 mL

GA-24 Fixed Angle, 35° (24 place)* GS6-TB-009

Outer row (rmax = 123 mm) 3750 1960 12 × 50 mL

Inner row (rmax = 108 mm) 3750 1700 12 × 50 mL

* No longer manufactured.

1-10
 2
Installation Requirements

! WARNING
This centrifuge weighs 65.4 kg (144.0 lb).
DO NOT attempt to lift or move it without
assistance from another person.

INSTALLATION REQUIREMENTS

! WARNING
Do not place the centrifuge near areas
containing flammable reagents or
combustible fluids. Vapors from these
materials could enter the centrifuge’s air
system and be ignited. Never bring any
flammable substances within the 30-cm (1-ft)
area surrounding the centrifuge. Maintain a
clearance envelope of 7.6 cm (3 in.) around an
operating centrifuge. No persons should be
within this clearance envelope while the
centrifuge is operating.

2-1
Installation Requirements

SPACE AND LOCATION REQUIREMENTS

Carefully remove the centrifuge and accessories from the shipping

container. Save the container and packing materials for possible
future relocation or storage of the centrifuge.

• Select a location away from heat-producing laboratory equipment,

with sufficient ventilation to allow for heat dissipation.

• Position the centrifuge on a level surface, such as a sturdy table or

laboratory bench, which is able to support the weight of the centri-
fuge (65.4 kg/144.0 lb) and resist vibration.

• Allow a 7.6-cm (3-in.) clearance at each side and the back of the
centrifuge to ensure sufficient air circulation. The centrifuge must
have adequate air ventilation to ensure compliance to local
requirements for vapors produced during operation. Dimensions
are shown in Figure 2-1.

cm
in.

94.0
37.0

39.4
15.5
SPEED

TIME

ACC/DEC

RPM
RCF
ROTOR
TIME

ACCEL ENTER
IMBALANCE
OPEN
DOOR DECEL
PULSE
FAST
STOP
® STOP
START
MADE
IN U.S.A.

58.4
23.0

54.6
21.5 7.6
3.0

Figure 2-1. Dimensions of the Spinchron DLX Centrifuge

2-2
 Installation Requirements

• Ambient temperature during operation should not exceed

35°C (95°F). Relative humidity should not exceed 95%
(noncondensing).

! CAUTION
Use the anti-rotation kit to secure the centrifuge
to the bench or table. The anti-rotation kit is
designed to minimize the possibility of injury or
damage that could result from centrifuge move-
ment in the event of a rotor mishap. Complete
instructions (SP-TB-007) for installing the anti-
rotation kit are included with the kit, which is
shipped with the centrifuge.

ELECTRICAL REQUIREMENTS

100-volt centrifuge. . . . . . . . . . . . . . . . 90–110 VAC, 12 A, 50/60 Hz

120-volt centrifuge. . . . . . . . . . . . . . . . . . 108–132 VAC, 10 A, 60 Hz
230-volt centrifuge. . . . . . . . . . . . . . . . . 207–253 VAC, 6.3 A, 50 Hz
Electrical supply . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Class I
Power cord . . . . . . . . . . . . . a 1.8-m (6-ft) power cord with grounded
plug is supplied with the centrifuge

➠ NOTE
In regions where a different plug is required to
meet local electrical and safety requirements,
contact your local Beckman Coulter
representative.

Make sure the voltage imprinted on the name rating plate affixed to
the back of the centrifuge agrees with the line voltage of the outlet
used. With the power switch in the off (O) position, plug in both ends
of the centrifuge power cord. If there is any question about voltage,
have a qualified service person measure it under load while the drive
is operating.

To reduce the risk of electrical shock, this equipment uses a

three-wire electrical cord and plug to connect the centrifuge to
earth-ground. To preserve this safety feature:

• Make sure that the matching wall outlet receptacle is properly

wired and earth-grounded.

2-3
Installation Requirements

• Never use a three-to-two wire plug adapter.

• Never use a two-wire extension cord or a two-wire non-grounding

type of multiple-outlet receptacle strip.

To ensure safety, the centrifuge should be wired to a remote emer-

gency switch (preferably outside the room where the centrifuge is
housed, or adjacent to the exit from that room), in order to disconnect
the centrifuge from the main power source in case of a malfunction.

TEST RUN

➠ NOTE
During transport between areas with varying
temperatures, condensation may occur inside the
centrifuge. Allow sufficient drying time before
running the centrifuge.

We recommend that you make a test run to ensure that the centrifuge
is in proper operating condition after shipment. See Section 3 for
instructions on operating the centrifuge.

➠ NOTE
After completing the test run, return the
preaddressed warranty card included with this
literature. This will validate the centrifuge
warranty and ensure your receipt of further
information regarding new accessories and/or
modifications as they become available.

NAME RATING PLATE

When corresponding with Beckman Coulter regarding your

Spinchron DLX centrifuge, always mention its model and serial
number. The serial number is shown on the name rating plate affixed
to the rear of the centrifuge, and is also on the inside of the door.

2-4
 3
Operation

This section contains operating procedures for the Spinchron DLX

centrifuge, using any of the rotors designed for use in this centrifuge.
A summary is provided at the end of this section. If you are an experi-
enced user of this centrifuge, you can turn to the summary for a quick
review of operating steps. Refer to the applicable rotor manual for
instructions on preparing the rotor for centrifugation.

! WARNING
The centrifuge must not be used in the
vicinity of flammable liquids or vapors, and
such materials should not be run in the
centrifuge. Never bring any flammable
substances within the 30-cm (1-ft) area
surrounding the centrifuge. During operation
you should not come within the 7.6-cm (3-in.)
clearance envelope except to adjust centrifuge
controls, if necessary. Do not lean on the
centrifuge or place items on the centrifuge
while it is operating.

! WARNING
Operator error or tube failure may generate
aerosols. Use only the appropriate rotors and
adapters. Do not run toxic, pathogenic, or
radioactive materials in this centrifuge
without taking appropriate safety pre-
cautions. Biosafe hoods should be used when
Risk Group II materials (as identified in
the World Health Organization Laboratory
Biosafety Manual) are handled; materials of
a higher group require more than one level
of protection.

3-1
Operation

RUN PREPARATION
The following detailed operating procedures are summarized at the
end of this section. If you are an experienced user of this centrifuge,
you can turn to the summary for a quick review of operating steps.
Refer to the applicable rotor manual for instructions on preparing the
rotor for centrifugation.

Action Result

1. Ensure that the power cord Power is available to the centrifuge.

is plugged into a wall
receptacle that provides
the correct voltage.

2. Press the power switch to Power is applied to the system and displays are lit.
on (I).

3. Move the manual lock lever

to the left (unlocked).

4. Press [OPEN DOOR], then lift Door remains in open position.

the door up.

! CAUTION
Never drop the rotor onto the centrifuge drive
shaft. The shaft can be damaged if the rotor is
dropped onto it or forced sideways.

5. Install the rotor or rotor yoke. The rotor is ready to load. (You can load fixed angle rotors either
Be sure the base is seated on before or after installing the rotor in the centrifuge.)
the drive shaft, with the pin
in the rotor groove.
➠ NOTE
Always run the rotor with a balanced load; fill
all four positions on a swinging bucket rotor
yoke with buckets and/or carriers.

3-2
 Operation

Action Result

6. Load the rotor according

to the rotor manual. ! WARNING
Do not substitute a metal fastener for the
plastic tie-down nut supplied with the rotor.
If a tie-down nut should come off the shaft
during centrifugation, the plastic nut
furnished will break apart in the chamber,
causing minimal damage to the centrifuge. A
loose metal object could substantially damage
the rotor, chamber, and lid, and could poten-
tially escape the chamber into the laboratory,
causing personal injury or property damage.

7. Firmly fasten the tie-down The rotor is secured to the shaft.

nut on the shaft. Do not
overtighten the nut.

Secondary (manual)
8. Close the door. Push firmly Lock Lever
down until you hear a SPEED
TIME
ACC/DEC

clicking (latching) sound

ENTER
ACCEL

TIME PULSE
DECEL
ROTOR
RCF
RPM
START
STOP
FAST
STOP

indicating that the door is

OPEN ™
CE DOOR
IMBALAN

latched.
®
IN U.S.A.
MADE

9. Move the manual lock lever

to the right (locked).

SETTING RUN PARAMETERS

When run parameters have been entered, as described below, they
will be retained in the centrifuge memory and can be recalled for
subsequent runs (only the last used parameters are retained). You
can then use the recalled parameters for the current run or alter them
as required.

3-3
Operation

SELECTING A ROTOR

Make sure the rotor is seated on the drive shaft and the tie-down nut
is tight before each run.

Action Result

1. Press [ROTOR]. The number of the rotor used in the previous run appears.

2. Press ▲ or ▼ until Rotor numbers are scrolled on the SPEED display.

the correct rotor appears.
SPEED

3. When the selected rotor If the rotor is the same as that used in the previous run, the last-used
number is displayed, parameter values for the selected rotor are displayed. You can use
press [ENTER]. these parameters or set new values for the run.

SPEED TIME ACC/DEC

SETTING RUN SPEED

You can set centrifuge speed, in either revolutions per minute (rpm)
or relative centrifugal field (RCF), for up to the maximum rated speed
of the selected rotor. During centrifugation, the SPEED display indi-
cates the actual run speed of the rotor (in rpm). You can check the
RCF during the run by pressing [RCF] while the centrifuge is
running.)

3-4
 Operation

Setting RPM

Action Result

SPEED

1. Press [RPM]. The last digit on the SPEED display flashes.

2. Press ▲ or ▼ to scroll The corresponding RCF will be automatically calculated by the

through speeds until the centrifuge.
required speed appears.

Setting RCF

Action Result
SPEED

1. Press [RCF]. The last digit on the SPEED display flashes.

2. Press ▲ or ▼ until the The corresponding rpm will be automatically calculated and the
required RCF appears. centrifuge will run at the calculated speed. (You can check the RCF
during the run by pressing [RCF] while the centrifuge is running.)

SETTING RUN TIME

You can set run time for either a timed run or continuous operation.

Action Result
TIME

1. Press [TIME]. The last digit on the TIME display flashes.

2. Press ▲ or ▼ until the If you enter a minutes parameter that exceeds 59, it is converted into
required time is displayed. hours.

• Timed run — when the TIME display reaches zero, the run ends.

• Continuous — the infinity (∞) symbol lights up and time elapsed

since the run start is displayed; the run continues until [STOP] or
[FAST STOP] is pressed.

3-5
Operation

SETTING ACCELERATION AND DECELERATION RATES

You can select one of nine acceleration and nine deceleration rates
that will maintain optimum separation of delicate samples. Select
rates depending on the type of run you are performing—for pelleting
runs, where sample mixing is not a concern, maximum brake can be
used. If running delicate gradients, a lower brake setting may be
needed. Acceleration and deceleration rates are listed in Table 3-1.

Table 3-1. Acceleration and Deceleration Rates (in Minutes:Seconds) to and from Maximum Speed.
Times shown are for the rotor’s maximum speed with the rotor fully loaded. (Times are approximate;
actual times will vary depending on the rotor load, run speed, and voltage fluctuations.)

Accel/ GS-3.8 GS-3.8A PTS-DLX GA-10 or GA-24

Decel
Rate Accel Decel Accel Decel Accel Decel Accel Decel

9 1:15 0:42 2:30 1:20 0:44 0:27 0:18 0:18

8 1:20 0:44 2:34 1:23 0:46 0:31 0:23 0:21
7 1:23 0:48 2:37 1:27 0:50 0:36 0:39 0:40

6 1:33 1:18 2:46 1:46 1:12 1:08 1:18 1:18

5 2:00 1:55 2:52 2:23 1:43 1:40 1:57 1:53
4 2:37 2:31 3:29 3:39 2:15 2:12 2:34 2:32

3 3:52 3:48 4:44 4:56 3:19 3:17 3:49 3:46

2 7:30 7:30 8:22 8:38 6:33 6:31 7:34 7:31
1 11:22 11:16 12:14 12:24 9:50 9:46 11:19 11:15

Setting an Acceleration Rate

Action Result
ACC/DEC

1. Press [ACCEL]. The ACC/DEC display flashes.

2. Press ▲ or ▼ until the

selected rate is displayed.

3-6
 Operation

Setting a Deceleration Rate

Action Result
ACC/DEC

1. Press [DECEL]. The ACC/DEC display flashes.

2. Press ▲ or ▼ until the

selected rate is displayed.

STARTING A RUN

➠ NOTE
For a short-duration run, press [ENTER] and then
press the [PULSE] key. The rotor will accelerate
to set speed and continue for as long as the key
is pressed. Deceleration, at maximum rate,
begins when you release the key.

You can start the run using the parameters in memory from a previous
run, or using new or changed parameters that you enter using the
procedure described above.

➠ NOTE
Long or repetitive runs may cause sample
heating because the centrifuge is not
refrigerated.

3-7
Operation

Action Result

1. Check that all parameters are

correct and the door is
closed.
! CAUTION
Do not lift or move the centrifuge while the rotor
is spinning.

2. Move the manual lock lever

to the right (locked position).

3. Press [ENTER], then press • The SPEED display indicates rotor speed in rpm. (You can check
[START]. the RCF by pressing the [RCF] key.)

• A blinking LED at the bottom of the TIME display indicates that

the run is in progress. This display also shows the remaining run
time (in hours and minutes) for a timed run; or the infinity (∞)
symbol and time elapsed since the run start. (When elapsed time
reaches 9 hours and 59 minutes during a continuous run, it resets
to 0 and continues counting.)

• The ACC/DEC display shows the deceleration rate selected for

the run.

! WARNING
Do not attempt to override the door interlock
system while the rotor is spinning.

CHANGING PARAMETERS DURING A RUN

While a run is in progress, run parameters (speed, time, and decelera-
tion rate) can be altered without stopping the run. Run duration can
also be changed from continuous to a specified time period, or from a
specified time period to continuous.

3-8
 Operation

➠ NOTE
The deceleration rate cannot be changed after
deceleration starts.

Use the parameter keys as described under ENTERING RUN

PARAMETERS, above, to change parameters. You must press [ENTER]
after you input each change. For example, you would change run
speed during centrifugation as follows:

Action Result
SPEED

1. Press [RPM]. The SPEED display flashes.

2. Press ▲ or ▼ until the

selected speed is displayed.

3. Press [ENTER]. The current speed will be displayed, changing to the new value as the
rotor accelerates or decelerates to the new speed selected. The centri-
fuge will automatically calculate the corresponding RCF.

STOPPING A RUN
A timed run ends automatically when the TIME display counts down
to zero. To end a run in progress for any reason:

Action Result

1. Press [STOP]. Normal deceleration begins, as selected by the deceleration rate.

(or)

Press [FAST STOP]. Deceleration begins at the maximum rate (see Table 3-1). The decel-
eration process cannot be interrupted; the centrifuge can be restarted
only after the rotor comes to a complete stop and the door is opened
and closed.

3-9
Operation

Action Result

2. After the rotor stops spinning

and the [OPEN DOOR] light
comes on, move the manual
! CAUTION
lock lever to the left The door interlock system will release the latch
(unlocked). and allow the door to be opened when the rotor
speed is 40 rpm or less. However, for operator
safety, do not open the door until the rotor has
come to a complete stop. Additionally, do not
attempt to override the door interlock system
while the rotor is spinning.

3. Press [OPEN DOOR], then lift

the door up.

UNLOADING

! CAUTION
If disassembly reveals evidence of leakage, you
should assume that some fluid escaped the rotor.
Apply appropriate decontamination procedures
to the centrifuge and accessories.

After completing a run, unload the rotor as described in the applicable

rotor manual. It is not necessary to remove the rotor from the centri-
fuge between runs unless the rotor or chamber bowl needs to be
cleaned. (If the rotor is left in the centrifuge between runs, make sure
the rotor is seated on the drive shaft and the tie-down nut is tight
before each run.)

➠ NOTE
Accumulations of sample, dust, and/or glass
particles from broken sample tubes can ruin
rotor pins and cause rotor vibrations. To prevent
these accumulations, remove the rotor and clean
the centrifuge bowl daily. See CLEANING in
Section 5.

3-10
 Operation

SUMMARY OF RUN PROCEDURES

1. Press the power switch to on (I).

2. Move the manual lock lever to the left (unlocked), then press
[OPEN DOOR] and lift the door up.

3. Install the rotor according to the applicable rotor manual. Always

run the rotor with a balanced load.

4. Close the centrifuge door and push firmly down on it until you
hear a clicking (latching) sound. Move the manual lock lever to
the right (locked).

5. Enter run parameters:

• Select a rotor number — [ROTOR], ▲ or ▼, [ENTER]

• Set run speed — [RPM], ▲ or ▼; or [RCF], ▲ or ▼

• Set run duration — [TIME], ▲ or ▼

• Set acceleration rate (1 through 9) — [ACCEL], ▲ or ▼

• Set deceleration rate (1 through 9) — [DECEL], ▲ or ▼

6. Check that all parameters are correct and the door is closed and
latched, then press [ENTER], then [START].

! CAUTION
Do not lift or move the centrifuge while the rotor
is spinning.

7. Wait for the set time to count down to zero, or end the run by
pressing [STOP] or [FAST STOP].

! WARNING
Never attempt to override the door interlock
system while the rotor is spinning.

8. After the rotor stops spinning and the [OPEN DOOR] light comes
on, move the manual lock lever to the left (unlocked). Press
[OPEN DOOR] to release the door latch. Lift the door to open it.

9. Unload the rotor according to the applicable rotor manual.

3-11
 4
Troubleshooting

This section lists possible malfunctions, together with probable

causes and corrective actions required. Maintenance procedures are
contained in Section 5. For any problems not covered here contact
Beckman Coulter Field Service.

➠ NOTE
It is your responsibility to decontaminate the
centrifuge, as well as any accessories, before
requesting service by Beckman Coulter Field
Service representatives.

USER MESSAGES

! CAUTION
If the message SEr appears on the display,
do not press any keys while the message is
displayed. Turn the centrifuge power off (O) and
back on (I) to clear the message. This message
indicates that you have inadvertently accessed
the service mode; pressing any keys while in
this mode could erase the centrifuge memory
and critically interfere with future operation.

If a problem occurs during operation, the rotor will decelerate to a

stop and an error code will appear on the SPEED display. Messages
may result from incorrect input (such as exceeding allowable speed
for a rotor) or from an equipment malfunction. Refer to Table 4-1 to

4-1
Troubleshooting

Table 4-1. Error Message Chart.

If the recommended action does not correct the problem, notify Beckman Coulter Field Service.

Error
Number Problem Result Recommended Action

1 through 22, Microprocessor or Deceleration to full stop; After rotor comes to a complete stop, turn
25 and 26, mechanical run cannot be restarted the power off (O), then back on (I) to reset.
28 through 30, malfunction
32, 36, 39 and 40,
44 through 60,
63 through 68

23 and 24, 27, 31, Microprocessor or Deceleration to full stop; After rotor comes to a complete stop, open
33 through 35, mechanical run cannot be restarted and close the centrifuge door, then restart.
37 and 38, malfunction
41 through 43, 62

69 through 77 Microprocessor Run cannot start until Turn the power off (O), then back on (I) to
malfunction error is cleared reset.

78 through 80 Error during closing Run cannot start 1. Remove debris in latch.
of door 2. Close lid quickly.
3. Turn the power off (O), then back on (I)
to reset.

81 “Door open” Maximum deceleration to After rotor comes to a complete stop,

detected during run full stop close the centrifuge door, turn the power
off (O), then back on (I) to reset.

82, 83 Door does not open — See access procedures, below.

determine the nature of the problem and recommended actions. If

you are unable to correct the problem, call Beckman Coulter Field
Service. To help diagnose and correct the problem, try to gather as
much information about the situation as you can:

• Write down the error number that appears on the display.

• Note the operating situation when the error occurred (rotor in use,
speed, load type, and so forth).

• Note any unusual environmental and/or operating conditions

(ambient temperature, voltage fluctuations, and so forth).

• Add any other information that may be helpful.

4-2
 Troubleshooting

OTHER POSSIBLE PROBLEMS

Possible malfunctions that may not be indicated by diagnostic
messages are described in Table 4-2, along with probable causes and
corrective actions required. Possible causes for each problem are
listed in the probable order of occurrence. Perform the recommended
corrective action in sequence, as listed. If you are unable to correct
the problem, call Beckman Coulter Field Service.

Table 4-2. Troubleshooting Chart

Problem Problem/Result Recommended Action

Imbalance LED 1. Rotor is out of balance 1. Check to be sure the rotor is in good condition
lights and rotor and is loaded symmetrically around the center
decelerates to stop of rotation, with containers of equal weight and
density (within 6 grams) opposite each other.
2. Centrifuge is misaligned 2. Align the centrifuge on the bench or table.
(tilted)
3. Centrifuge was moved 3. After the rotor comes to a complete stop, open
during operation and close the centrifuge door, then restart.
4. Drive error (mechanical damage) 4. Call Beckman Coulter Field Service.

Rotor cannot 1. Line voltage below rating 1. Have a qualified service person measure line
achieve set speed voltage while the instrument is operating.
2. Electrical failure 2. Make sure both ends of the power cord are
securely connected; call Beckman Coulter Field
Service
3. Motor failure 3. Call Beckman Coulter Field Service.

Door will not open 1. Rotor spinning 1. Wait until the rotor stops.
2. Manual lock lever in locked 2. Move the lever to the left (unlocked).
position.
3. Power not on 3. Plug in the power cord; turn power on (I).
4. Source power failure 4. See access procedures, below.
5. Latch stuck 5. See access procedures, below.

Displays are blank 1. Power not on 1. Plug in the power cord; turn power on (I).
2. Electrical failure 2. Make sure both ends of the power cord are
securely connected; call Beckman Coulter Field
Service.
3. Fuse blown 3. Call Beckman Coulter Field Service.

4-3
Troubleshooting

ACCESSING THE ROTOR IN CASE OF POWER FAILURE

If the facility power fails only momentarily, the centrifuge will
resume operation when power is restored and the rotor will return to
set speed. However, if the rotor comes to a complete stop you will
have to restart the run when the power is restored. In the event of an
extended power failure, it may be necessary to trip the door-locking
mechanism manually to remove the rotor and retrieve your sample.

Action Result

1. Turn the power switch to off No displays or LEDs are lit.

(O) and disconnect the power
cord from the main power
source.

2. Make sure that the rotor is No sound or vibration comes from the centrifuge.
not spinning.

! WARNING
Never attempt to override the door interlock
system while the rotor is spinning.

3. Move the manual lock lever

to the left (unlocked).

4-4
 Troubleshooting

Action Result

4. Insert the supplied hex

wrench (929317) into the
override key slot in the left Secondary (manual)
Lock Lever
side panel.
ACC/DEC

TIME

SPEED

ENTER
ACCEL

TIME PULSE
DECEL
ROTOR
RCF
RPM
START
STOP
FAST
STOP

OPEN ™
CE DOOR
IMBALAN

®
IN U.S.A.
MADE

5. Turn the hex wrench (either The latch “clicks” or the door pops open.
direction).
If the rotor is still spinning, close the door and wait until it stops
before attempting to remove it.

! WARNING
Never try to slow or stop the rotor by hand.

4-5
 5
Care and Maintenance

For maintenance not covered in this manual, contact Beckman

Coulter Field Service.

! WARNING
Any maintenance procedure or servicing of
this equipment that requires removal of any
covers can expose parts that involve the risk
of electric shock or personal injury. Make
sure that the power switch is off (O) and the
centrifuge is disconnected from the main
power source, and refer such servicing to
qualified personnel.

GENERAL MAINTENANCE
Perform the following procedures regularly to ensure satisfactory
performance and long service life of the centrifuge.

• At least once a week (depending on usage) inspect the interior of

the rotor chamber for accumulations of sample, dust, and glass
particles from broken sample tubes. Clean as required (see
CLEANING, below), as these accumulations can result in rotor
vibrations.

• Regularly check the air intake and exhaust vents for obstructions.
Keep vents clear and clean.

5-1
Care and Maintenance

• To prevent the rotor from sticking, lubricate the drive shaft with
Spinkote (306812) at least once a month, and after each cleaning.

• Refer to the applicable rotor manual for instructions on the care of

rotors and other components and accessories.

➠ NOTE
Before using any cleaning or decontamination
methods except those recommended by the
manufacturer, users should check with the
manufacturer that the proposed method will not
damage the equipment.

CLEANING
Frequent cleaning will ensure proper operation and prolong the life
of the centrifuge. Always clean up spills when they occur to prevent
corrosives or contaminants from drying on component surfaces.

• Remove the rotor from the centrifuge weekly and clean the bowl,
drive shaft, shaft cavity, threads, and the tie-down nut using a mild
detergent such as Beckman Solution 555™ (339555) and a soft
brush. Dilute the detergent 10 to 1 with water. Rinse thoroughly
and dry completely. Lubricate the drive shaft with Spinkote after
cleaning.

• Keep the interior of the rotor chamber clean and dry by frequent
Air-Intake wiping with a cloth or paper towel. To prevent accumulations of
Filters
sample, dust, and/or glass particles from broken sample tubes from
damaging rotor pins (causing rotor vibrations), remove the rotor
and clean the centrifuge chamber daily. Spray the centrifuge
chamber with an antistatic solution and wipe it clean. (Or use an
antistatic wipe.)

• Clean the centrifuge case and door by wiping with a damp cloth or
washing with Solution 555. Do not use acetone or other solvents.

• Remove the air-intake filters and inspect for dust and dirt. Wash
RPM
SPEED

RCF
ROTOR
TIME

ACC/DEC
them using a mild soap or detergent, such as Solution 555. Rinse
thoroughly and squeeze excess water out, then air-dry before
TIME

ACCEL ENTER
IMBALANC
E OPEN
DOOR DECEL
PULSE
FAST
STOP
® STOP
START
MADE
IN U.S.A.

reinserting.
™

5-2
 Care and Maintenance

DECONTAMINATION
If the centrifuge and/or accessories are contaminated with radioactive
or pathogenic solutions, perform appropriate decontamination proce-
dures. Refer to Chemical Resistances (publication IN-175) to be sure
the decontamination method will not damage any part of the
centrifuge.

STERILIZATION AND DISINFECTION

The centrifuge is finished with urethane paint. Ethanol (70%)* may
be used on this surface. See Chemical Resistances for more informa-
tion regarding chemical resistance of centrifuge and accessory
materials.

While Beckman Coulter has tested this method and found that it does
not damage the centrifuge, no guarantee of sterility or disinfection is
expressed or implied. When sterilization or disinfection is a concern,
consult your laboratory safety officer regarding proper methods
to use.

TUBE BREAKAGE
If a glass tube breaks, and all the glass is not contained in the bucket
or rotor, thoroughly clean the interior of the chamber bowl.

! CAUTION
Be careful when examining or cleaning the
gasket or chamber, as sharp glass fragments
may be embedded in their surfaces.

• Examine the gasket to make sure that it is free of glass particles.

Carefully remove any glass particles that may remain. If glass is
under the gasket, call Beckman Coulter Field Service to replace
the gasket.

• Carefully wipe away any glass particles that remain in the bowl.

* Flammability hazard. Do not use in or near an operating centrifuge.

5-3
Care and Maintenance

STORAGE AND TRANSPORT

If you are shipping both the centrifuge and a rotor, remove the rotor
from the centrifuge and pack it in its original shipping container. A
rotor left in the centrifuge can bend the drive shaft if the package is
jarred during shipment.

STORAGE

Before storing a centrifuge for an extended period remove the rotor.

Return the centrifuge to its original shipping container to protect it
from dust and dirt. Temperature and humidity conditions for storage
should meet the operating environment requirements described under
SPECIFICATIONS in Section 1.

RETURNING A CENTRIFUGE

Before returning a centrifuge or accessory for any reason, prior

RGA permission (a Returned Goods Authorization form) must be obtained
from Beckman Coulter, Inc. Contact your local Beckman Coulter
office to obtain the RGA form and for packaging and shipping
instructions.

To protect our personnel, it is the customer’s responsibility to ensure

that all parts are free from pathogens and/or radioactivity. Steriliza-
tion and decontamination must be done before returning the parts.

All parts must be accompanied by a note, plainly visible on the out-

side of the box, stating that they are safe to handle and that they are
not contaminated with pathogens or radioactivity. Failure to attach
this notification will result in return or disposal of the items without
review of the reported problem.

5-4
 Care and Maintenance

SUPPLY LIST

➠ NOTE
Publications referenced in this manual can be
obtained by calling Beckman Coulter at 1-800-
742-2345 in the United States, or by contacting
your local Beckman Coulter office.

Contact Beckman Coulter Sales (1-800-742-2345 in the United

States; worldwide offices are listed at the back of this manual) for
information about ordering parts and supplies. For your convenience,
a partial list is given below.

REPLACEMENT PARTS

Neoprene boot . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 358009

Rotor tie-down nut . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 366642
Tie-down nut O-ring (Buna N rubber) . . . . . . . . . . . . . . . . . . . . . . . . 927600
Torquing bar . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 356036
Hex wrench (latch release) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 929317
Power cord (60-Hz, 120 VAC) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 889097
Power cord (50-Hz, 230 VAC) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 355810

SUPPLIES

Spinkote lubricant (2 oz) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 306812

Silicone vacuum grease (1 oz) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 335148
Beckman Solution 555 (1 qt) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 339555
Magnetized document holder . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 964987

5-5
 SPINCHRON DLX CENTRIFUGE WARRANTY

Subject to the exceptions and upon the conditions specified CONDITIONS

below and the warranty clause of the Beckman Coulter terms
and conditions in effect at the time of sale, Beckman Coulter Beckman Coulter makes no warranty concerning products or
agrees to correct either by repair or, at its election, by replace- accessories not manufactured by it. In the event of failure of
ment, any defects of material or workmanship which develop any such product or accessory, Beckman Coulter will give
within one (1) year after delivery of a SPINCHRON DLX reasonable assistance to the Buyer in obtaining from the
centrifuge (the product), to the original buyer by Beckman respective manufacturer whatever adjustment is reasonable in
Coulter or by an authorized representative, provided that light of the manufacturer’s own warranty.
investigation and factory inspection by Beckman Coulter
discloses that such defect developed under normal and proper Beckman Coulter shall be released from all obligations under
use. all warranties, either expressed or implied, if the product(s)
covered hereby are repaired or modified by persons other
Some components and accessories by their nature are not than its own authorized service personnel, unless such repair
intended to and will not function for as long as one (1) year. A in the sole opinion of Beckman Coulter is minor, or unless
complete list of such components or accessories is maintained such modification is merely the installation of a new Beck-
at the factory and at each Beckman Coulter District Sales man Coulter plug-in component for such product(s).
Office. The lists applicable to the products sold hereunder
shall be deemed to be part of this warranty. If any such com-
ponent or accessory fails to give reasonable service for a rea- DISCLAIMER
sonable period of time, Beckman Coulter will repair or, at its
election, replace such component or accessory. What consti- IT IS EXPRESSLY AGREED THAT THE ABOVE WAR-
tutes either reasonable service and a reasonable period of time RANTY SHALL BE IN LIEU OF ALL WARRANTIES OF
shall be determined solely by Beckman Coulter. FITNESS AND OF THE WARRANTY OF MERCHANT-
ABILITY AND THAT NEITHER BECKMAN COULTER,
INC. NOR ITS SUPPLIERS SHALL HAVE ANY
REPLACEMENT LIABILITY FOR SPECIAL OR CONSEQUENTIAL
DAMAGES OF ANY KIND WHATSOEVER ARISING
Any product claimed to be defective must, if requested by OUT OF THE MANUFACTURE, USE, SALE, HAN-
Beckman Coulter, be returned to the factory, transportation DLING, REPAIR, MAINTENANCE, OR REPLACEMENT
charges prepaid, and will be returned to Buyer with the trans- OF THE PRODUCT.
portation charges collect unless the product is found to be
defective, in which case Beckman Coulter will pay all trans-
portation charges.
Beckman Coulter, Inc. • 250 S. Kraemer Blvd. • Brea, California 92821
Sales and Service: 1-800-742-2345 • Internet: www.beckmancoulter.com

©2009 Beckman Coulter, Inc.

All rights reserved