PRINCETON

CHROMATOGRAPHY INC

ABOUT US

Princeton Chromatography Inc., based in Cranbury, New Jersey, USA, was

founded in 1994. We have over 40 years experience in chromatography and
are one of the earliest developers of novel commercial SFC phases.

Princeton Chromatography Inc. delivers the highest quality, cutting edge products
backed by unmatched technical support. From SFC to HPLC, analytical to
preparative, all columns are manufactured on-site and subjected to rigid
quality standards.

Every SFC and HPLC column is tested and shipped with an original chromatogram.
All stationary phase media are bonded at our facility, so the quality and reproducibility
of each batch can be closely monitored. All columns are packed and tested on-site
by our team of production specialists to ensure the highest level of satisfaction for
our customers.

Princeton Chromatography manufactures a wide range of phases for SFC and both
reversed-phase and normal-phase HPLC. We are one of the world leaders in the
development of innovative SFC phases.

With so many stationary phases and column dimensions to choose from, we can make
your scale up easy and worry free. From 2.0 mm screening columns for LC-MS
to kilograms of bulk media, we are with you every step of the way.

PRINCETON CHROMATOGRAPHY INC. OFFERS:

• Widest selection of bonded achiral SFC phases on the market
• Wide range of HPLC phases for analytical and preparative applications
• Bulk materials for HPLC
• Custom column packing services for SFC, SMB and preparative applications
• Chiral media repacking services
• Small scale purification service

PrincetonSFC SFC Columns.........................................................................................Pages 3-11

PrincetonSPHER HPLC Columns............................................................................. Pages 12-17
Other Services ...................................................................................................................... Page 18
 PRINCETON
CHROMATOGRAPHY INC

SFC Overview
Supercritical Fluid Chromatography (SFC) is a viscosity and lower surface tension. The lower viscosity




 



   
      )''#/! *6*1 +#/ 1)$! %#"2)1 $# 78. ')&6 $# 0)!$1
as the mobile phase. Although SFC has been around for methods. Any solute soluble in methanol or a less polar
some time, its adaptation as an orthogonal technique to organic solute will elute in SFC. For polar compounds, a
HPLC, particularly in the pharmaceutical industry, has seen more polar additive may also be added to the mobile phase to
an increase over the last few years. This interest has been facilitate elution and improve peak shape.
fuelled by the increasing requirement for high throughput
and a desire for ‘green’ techniques. Large reductions in Packed column SFC has developed from HPLC



 


    

  
  instrumentation and columns. The mobile phase is kept
decreased sample processing and drying-down times supercritical by an electronically controlled variable pressure
in preparative SFC, as well as providing cost and safety restrictor positioned after the detector.



Key Benefits of SFC
• Fast analyses
• Reduced solvent consumption
9 :;<= >?@ ABCDE F?EE;GHD
• Lower cost per sample
• Compatible with MS
• Excellent for preparative separations

Retention mechanisms in SFC are currently not well

understood, but depend mainly on the nature of the
stationary phase. SFC is generally seen as a normal-phase
technique, predominantly using polar stationary phases, along
with less polar mobile phases. However, hydrophobic C18
bonded silica phases offer a reversed-phase retention mode
and different selectivity. In fact any HPLC phase can be used
Figure 1. CO2 Phase Diagram
for SFC, in addition to the wide range of available phases
EFDI;JIBHHK LDE;<MDL N?A OPQR
  2 ! "#!$ %#""#&'( ! )! $* ")& +
& ,-. /$* $* )$#& #0 ) "#1 !%* )! "$*)&#' The table below shows typical starting conditions for
(typically 2% to 60% v/v) to aid elution of very polar or ionic an achiral analytical SFC assay. For basic analytes, a
%#"2#&!3 4* "#1 "21#5! $* !#'5)$&6 2#/1 pyridine based column is a good starting point. For acidic
#0 $* !21%1$%)' + )& &*)&%! $* !'%$5$( #0 $* compounds, a diol type column may be more retentive.
!2)1)$#&3 ,21%1$%)' +! %)& *)5 !#'5)$&6 2#/1! Neutral compounds do not generally require an additive
similar to organic solvents but with higher diffusivity, lower for elution.

Typical Analytical Conditions for Achiral SFC

Stationary Phase Silica, Diol, 2-Ethylpyridine etc.
Column Dimensions
Length 5, 10, 75, 150, 250 mm
i.d. 2.0, 3.0, 4.0, 4.6 mm
Mobile Phase Flow rate: 1 – 5 ml/min
ST2
UVWXYZ[ Methanol
Additive 1* 0.1% Diethylamine or 15mM ammonium acetate (for basic compounds)
0.1% TFA or 0.1% formic acid (for acidic compounds)
Gradient \ ] \^_ `VWXYZ[
Pressure 100 - 200 bar
Temperature 35 – 45°C
Detection UV, MS, ELSD, CAD

* 1 - 5% water may also be added if required

3
PRINCETON
CHROMATOGRAPHY INC

PrincetonSFC Phases
Achiral SFC separations typically require ‘normal-phase’ type polar stationary phases, such as silica,
amino, cyano and diol. Although these phases are adequate for many applications, there is still a need for
additional polar phases to meet the demands of difficult separations.

At Princeton Chromatography we have led the way in developing a series of novel amide, urea and pyridine phases
that enhance the capability of the SFC technique by providing increased selectivity and loading capacity. Princeton



 
       
  
  




 
 
2001 and later the 4-Ethylpyridine phase. PrincetonSFC 2-Ethylpyridine is non-endcapped and has become the column
of choice for achiral SFC analysis of basic compounds. This phase generally requires no addition of amines to the eluent,
producing excellent peak shape and reproducibility.

A diol phase is a popular and versatile stationary phase for SFC. We have also developed several different hydroxylated
stationary phases, most notably the HA Series, which offer excellent selectivity and retention characteristics. These phases
are a great starting point for impurity analysis.

Pore Size Particle Size

Phase Structure Phase Code
(Å) (µm)

60 3, 5, 10
2-Ethylpyridine 100 2.5, 3, 5, 10 77
300 3, 5, 10

60 3, 5, 10
Silica 10
100 3, 5, 10

60 3, 5, 10
Cyano 07
100 3, 5, 10

 60
100
3, 5, 10
3, 5, 10
09

 60 5, 10 79

 !" 60
100
3, 5, 10
3, 5, 10
86

60 3, 5, 10
Amino 08
100 3, 5, 10

DEAP (Diethylamino) 60 3, 5, 10 75

Benzamide 100 3, 5, 10 76

PA (Propylacetamide) 60 3, 5, 10 80

PPU (Propylpyridylurea) 100 3, 5, 10 82

4
 PRINCETON
CHROMATOGRAPHY INC

Pore Size Particle Size

Phase Structure Phase Code
(Å) (µm)

Propylurea 100 3, 5, 10 36

DNP (Dinitrophenyl) 100 3, 5, 10 93

Pyridine Amide 60 3, 5, 10 83

60 3, 5, 10
4-Ethylpyridine 90
100 3, 5, 10

Methane Sulfonamide 60 3, 5, 10 91

Benzene Sulfonamide 100 3, 5, 10 92

4-Nitrobenzene Sulfonamide 100 3, 5, 10 95

4-Fluorobenzene Sulfonamide 100 3, 5, 10 96

HA-Pyridine 60 3, 5, 10 87

HA-Dipyridyl 100 3, 5, 10 88

HA-DEA (Diethylamino) 60 3, 5, 10 65

HA-DHP (Dihydroxypropyl) 100 3, 5, 10 69

3,5-Dihydroxyphenyl 100 3, 5, 10 45

5
PRINCETON
CHROMATOGRAPHY INC

PrincetonSFC Analytical Columns

PrincetonSFC analytical columns are available with lengths of 50 to 250 mm and internal diameters of 2.0, 3.0, 4.0
and 4.6 mm. All PrincetonSFC columns are individually quality assured by SFC. Available particle sizes are 3, 5 and
10 μm, with an additional 2.5 μm particle size phase available for the PrincetonSFC 2-Ethylpyridine columns. Pore
sizes of 60, 100 and 300Å (for 2-Ethylpyridine phase) are available.

Ordering Information
In order to determine the part number of your required PrincetonSFC analytical column, just insert the phase code
X from the table below into the part number table (e.g. for a 150 x 4.6 mm 2-Ethylpyridine column with a pore size
of 60 Å and a particle size of 5 μm, the part number is 150046-01577).

PHASE CODES:
Phase Code X Phase Code X Phase Code X
2-Ethylpyridine 77 Benzamide 76 Benzene Sulfonamide 92
Silica 10 PA 80 4-Nitrobenzene Sulfonamide 95
Cyano 07 PPU 82 4-Fluorobenzene Sulfonamide 96

 09 Propylurea 36 HA-Pyridine 87

 79 DNP 93 HA-Dipyridyl 88

 86 Pyridine Amide 83 HA-DEA 65
Amino 08 4-Ethylpyridine 90 HA-DHP 69
DEAP 75 Methane Sulfonamide 91 3,5-Dihydroxyphenyl 45

Column Column i.d. (mm)

Pore Size Particle
Length
(Å) Size (µm)
(mm) 2.0 3.0 4.0 4.6
60 3 50 050020-013X 050030-013X 050040-013X 050046-013X
60 3 75 075020-013X 075030-013X 075040-013X 075046-013X
60 3 100 100020-013X 100030-013X 100040-013X 100046-013X
60 3 150 150020-013X 150030-013X 150040-013X 150046-013X
60 3 250 250020-013X 250030-013X 250040-013X 250046-013X
60 5 50 050020-015X 050030-015X 050040-015X 050046-015X
60 5 75 075020-015X 075030-015X 075040-015X 075046-015X
60 5 100 100020-015X 100030-015X 100040-015X 100046-015X
60 5 150 150020-015X 150030-015X 150040-015X 150046-015X
60 5 250 250020-015X 250030-015X 250040-015X 250046-015X
60 10 50 050020-010X 050030-010X 050040-010X 050046-010X
60 10 75 075020-010X 075030-010X 075040-010X 075046-010X
60 10 100 100020-010X 100030-010X 100040-010X 100046-010X
60 10 150 150020-010X 150030-010X 150040-010X 150046-010X
60 10 250 250020-010X 250030-010X 250040-010X 250046-010X
100 3 50 050020-033X 050030-033X 050040-033X 050046-033X
100 3 75 075020-033X 075030-033X 075040-033X 075046-033X
100 3 100 100020-033X 100030-033X 100040-033X 100046-033X
100 3 150 150020-033X 150030-033X 150040-033X 150046-033X
100 3 250 250020-033X 250030-033X 250040-033X 250046-033X
100 5 50 050020-035X 050030-035X 050040-035X 050046-035X
100 5 75 075020-035X 075030-035X 075040-035X 075046-035X
100 5 100 100020-035X 100030-035X 100040-035X 100046-035X
100 5 150 150020-035X 150030-035X 150040-035X 150046-035X
100 5 250 250020-035X 250030-035X 250040-035X 250046-035X
100 10 50 050020-030X 050030-030X 050040-030X 050046-030X
100 10 75 075020-030X 075030-030X 075040-030X 075046-030X
100 10 100 100020-030X 100030-030X 100040-030X 100046-030X
100 10 150 150020-030X 150030-030X 150040-030X 150046-030X
100 10 250 250020-030X 250030-030X 250040-030X 250046-030X
300 3 50 050020-083X 050030-083X 050040-083X 050046-083X
300 3 75 075020-083X 075030-083X 075040-083X 075046-083X
300 3 100 100020-083X 100030-083X 100040-083X 100046-083X
300 3 150 150020-083X 150030-083X 150040-083X 150046-083X
300 3 250 250020-083X 250030-083X 250040-083X 250046-083X
300 5 50 050020-085X 050030-085X 050040-085X 050046-085X
300 5 75 075020-085X 075030-085X 075040-085X 075046-085X

Guard cartridges are available for PrincetonSFC analytical columns – please enquire.

6
 PRINCETON
CHROMATOGRAPHY INC

PrincetonSFC Analytical Columns (continued)

Column Column i.d. (mm)
Pore Size Particle
Length
(Å) Size (µm)
(mm) 2.0 3.0 4.0 4.6
300 5 100 100020-085X 100030-085X 100040-085X 100046-085X
300 5 150 150020-085X 150030-085X 150040-085X 150046-085X
300 5 250 250020-085X 250030-085X 250040-085X 250046-085X
300 10 50 050020-080X 050030-080X 050040-080X 050046-080X
300 10 75 075020-080X 075030-080X 075040-080X 075046-080X
300 10 100 100020-080X 100030-080X 100040-080X 100046-080X
300 10 150 150020-080X 150030-080X 150040-080X 150046-080X
300 10 250 250020-080X 250030-080X 250040-080X 250046-080X
Guard cartridges are available for PrincetonSFC analytical columns – please enquire.

PrincetonSFC Semi-preparative and

Preparative SFC Columns
All Princeton semi-preparative and preparative SFC columns are packed using the same high quality bonded phases as
the corresponding analytical columns, making scale up from analytical dimensions seamless and straightforward. Princeton
preparative columns are available with internal diameters from 7.8 to 50.0 mm and in lengths from 50 mm to 250 mm.
All columns are quality controlled by SFC and individual SFC documentation is included with each column.

Ordering Information
Part numbers are determined using the same phase codes as for analytical columns (see page 4 for phase code table).
e.g. for a 250 x 21.2 mm Propylurea column with a pore size of 100 Å and particle size of 10 μm, the part number is 250212-
03036

PrincetonSFC Semi-preparative and Preparative SFC Columns – Part Numbers

Column Column i.d. (mm)
Pore Size Particle
Length
(Å) Size (µm)
(mm) 7.8 10.0 21.2 30.0 50.0
60 5 50 050078-015X 050100-015X 050212-015X 050300-015X 050500-015X
60 5 100 100078-015X 100100-015X 100212-015X 100300-015X 100500-015X
60 5 150 150078-015X 150100-015X 150212-015X 150300-015X 150500-015X
60 5 250 250078-015X 250100-015X 250212-015X 250300-015X 250500-015X
60 10 50 050078-010X 050100-010X 050212-010X 050300-010X 050500-010X
60 10 100 100078-010X 100100-010X 100212-010X 100300-010X 100500-010X
60 10 150 150078-010X 150100-010X 150212-010X 150300-010X 150500-010X
60 10 250 250078-010X 250100-010X 250212-010X 250300-010X 250500-010X
100 5 50 050078-035X 050100-035X 050212-035X 050300-035X 050500-035X
100 5 100 100078-035X 100100-035X 100212-035X 100300-035X 100500-035X
100 5 150 150078-035X 150100-035X 150212-035X 150300-035X 150500-035X
100 5 250 250078-035X 250100-035X 250212-035X 250300-035X 250500-035X
100 10 50 050078-030X 050100-030X 050212-030X 050300-030X 050500-030X
100 10 100 100078-030X 100100-030X 100212-030X 100300-030X 100500-030X
100 10 150 150078-030X 150100-030X 150212-030X 150300-030X 150500-030X
100 10 250 250078-030X 250100-030X 250212-030X 250300-030X 250500-030X
300 5 50 050078-085X 050100-085X 050212-085X 050300-085X 050500-085X
300 5 100 100078-085X 100100-085X 100212-085X 100300-085X 100500-085X
300 5 150 150078-085X 150100-085X 150212-085X 150300-085X 150500-085X
300 5 250 250078-085X 250100-085X 250212-085X 250300-085X 250500-085X
300 10 50 050078-080X 050100-080X 050212-080X 050300-080X 050500-080X
300 10 100 100078-080X 100100-080X 100212-080X 100300-080X 100500-080X
300 10 150 150078-080X 150100-080X 150212-080X 150300-080X 150500-080X
300 10 250 250078-080X 250100-080X 250212-080X 250300-080X 250500-080X

Guard cartridges are available for PrincetonSFC preparative columns – please enquire.
7
PRINCETON
CHROMATOGRAPHY INC

SFC Applications
Selectivity of PrincetonSFC 2-Ethylpyridine, PPU and DIOL
2

PrincetonSFC
2-Ethylpyridine
4
1

3 6
5
Column Dimensions: 250 x 4.6 mm
Eluent:
2 - CH3
 
Flow Rate: 2.0 ml/min
0 2 4 6 8
Minutes
10 12 14 16
Detection: UV at 230 nm
1. Ibuprofen
2 2. Aspirin
1 PrincetonSFC PPU 3. Ketoprofen
4 4. Indomethacin
(Propylpyridylurea) 5. Diclofenac
6. Sulindac
3
5
6
Changing stationary
phase is a powerful tool
for altering selectivity
0 2 4 6 8 10 12 14 16 18
Minutes

2
1 2 4
4
3 5

1
5
3
6 PrincetonSFC DIOL

0 2 4 6 8 10 12 14 16 18
Minutes

8
 PRINCETON
CHROMATOGRAPHY INC

SFC Applications
Separation of Four β-Blockers
Column: PrincetonSFC 2-Ethylpyridine, 60 Å, 5 μm
1 2 Catalog No: 250046-01577
Dimensions: 250 x 4.6 mm
Eluent:
2 - CH3
 
Additive: 0.1% N,N-diisopropylethylamine
3 4 Flow Rate: 5 ml/min
Temperature: 40°C
Detection: UV at 220 nm
Injection Volume: 4 μl
1. Metopropol tartrate 7.3 mg/ml
2. dl-Propranolol HCI 2.5 mg/ml
3. Acebutolol HCI 10.8 mg/ml
0 2 4 6 8 10 12 14 16 18 4. Pindolol 4.8 mg/ml
Minutes

Diuretic Compounds
Column: PrincetonSFC Benzamide, 100 Å, 5 μm 4
Catalog No: 250046-03576
Dimensions: 250 x 4.6 mm 1
Eluent:
2 - CH3
 


Flow Rate: 2 ml/min 2 3
Temperature: 40°C
Detection: UV at 220 nm
1. Diazoxide
 
 
4. Hydrochlorothiazide 0 1 2 3 4 5 6 7 8 9 10
Minutes

Separation of Basic Compounds

Column: PrincetonSFC Propylurea, 100 Å, 5 μm
Catalog No: 150046-03536
2 Dimensions: 150 x 4.6 mm
Eluent:
2 - CH3
 
Additive: 0.1% diethylamine
Flow Rate: 4.0 ml/min
3 Detection: UV at 254 nm
1 1. 7-Azaindole
2. 2-Benzylamino-4-methylpyridine
4
5 6 8
3. 2H-Pyrido[3,2,b]-1,4-oxazin-3(4H)-one
4. 4-Azabenzimidazole
7 5. Niacinamide
6. 1-Aminoisoquinoline
7. 2,6-Diaminopyridine
0 1 2 3 4 5 6 7 8 9 8. 1-(2-Pyridyl)piperazine
Minutes

9
PRINCETON
CHROMATOGRAPHY INC

SFC Applications

4 Separation of β-Blockers
Column: PrincetonSFC Silica, 5 μm
Dimensions: 250 x 4.6 mm
Eluent:
2 - CH3
 
5 Additive: 0.1% TEA
1 Flow Rate: 2.0 ml/min
Detection: UV at 273 nm
1. Timolol
2

 
3 3. Metroprolol
4. Propranolol
6 5. Pindolol
6. Acebutolol
7 7. Atenolol

0 2 4 6 8 10 12 14 16 18 20 22
Minutes

SFC Separation of Test Compounds on

PrincetonSFC Methane Sulfonamide 4
Column: PrincetonSFC Methane Sulfonamide, 5 μm
Dimensions: 250 x 4.6 mm
12
Eluent:
2 - CH3
 
Additive: 0.2% diethylamine
Flow Rate: 2.35 ml/min
Detection: UV at 254 nm
7
1. Caffeine 6. 5-Fluorouracil
2. Theophylline 7. Adenine 6
3. Theobromine
4. Thymine
8. Hypoxanthine
9. 5-Fluorocytosine
3 8 9 1
5. Uracil 10. Cytosine
0 2 4 6 8 10 12
Minutes

Separation of Steroids
1 4 Column: PrincetonSFC Benzamide
Dimensions: 250 x 4.6 mm
3 Eluent:
2 - CH3
 
Flow Rate: 2 ml/min
Temperature: 40°C
2 Detection: UV at 254 nm
1. Corticosterone
2. Cortisone
3. Prednisone
4. Prednisolone
0 2 4 6 8 10 12 14 16
Minutes

10
 PRINCETON
CHROMATOGRAPHY INC

SFC Applications

Test Mixture on PrincetonSFC

2CN:DIOL 3
20000
Column:


     μm
Dimensions: 150 x 4.6 mm 6
Gradient: 0-1 mins 5% CH3   ! 1 4
1-18 min 5%-40% CH3 2 11 1#
18-22 min 40% CH3 7
5 9
22-30 min - initial conditions 0
Flow Rate: 3 ml/min 12
Temperature: 40°C 8
Detection: UV at 254 nm
1. Caffeine 7. Prednisone
2. Theophylline 8. Hypoxanthine
-20000
3. Thymine 9. Hydrocortisone
4. Uracil 10. Sulfamerazine
5. Fenoprofen 11. Sulfamethoxazole 0 2 4 6 8 10 12 14 16
6. Flurbiprofen 12. Sulfaguanidine Minutes

Separation of Antimicrobials
2 Column: PrincetonSFC Propylacetamide
Dimensions: 250 x 4.6 mm
1 Eluent:
2 - CH3 " !
Flow Rate: 2 ml/min
3 Temperature: 40°C
Detection: UV at 254 nm
1. Furazolidone
2. Sulfadimethoxine
3. Sulfaquinoxiline

0 1 2 3 4 5 6 7 8
Minutes

1
Separation of Pyridine Amides
Column: PrincetonSFC 2-Ethylpyridine. 100 Å, 5 μm
Dimensions: 150 x 4.6 mm
Eluent:
2 – CH3 " ! 2
Flow Rate: 4.0 ml/min 3
Temperature: 40°C
Detection: UV at 220 nm
1. Picolinamide
2. Niacinamide
0 0.2 0.4 0.6 0.8 1.0 1.2 1.4 1.6 1.8
3. Isonicotinamide
Minutes

11
PRINCETON
CHROMATOGRAPHY INC

PrincetonSPHER HPLC Phases

At Princeton Chromatography we have been developing and manufacturing HPLC columns for over
20 years. We offer a wide variety of bonded phases in a range of particle and pore sizes.

PrincetonSPHER-60 Series phases (60 Å pore size) are optimal for compounds under 1000 Da molecular weight.
In most cases, the high surface area (500 m2/g) leads to longer retention times than with the corresponding 100 Å
materials. All PrincetonSPHER-60 columns are packed with high purity silica media that are custom bonded at our
facility to ensure the highest standards of quality.

PrincetonSPHER-100 Series columns are our most versatile, with a large variety of phases available. With a pore
size of 100 Å, an average surface area of 325 m2/g and excellent batch-to-batch reproducibility, these columns are
excellent for method development.

PrincetonSPHER-300 Series (300 Å pore size) are the columns of choice for reversed-phase separations of large

  

       
 
 

    
    

 




  
 
 


 


PrincetonSPHER-HTS is a 60 Å pore size C12 phase designed for High Throughput Screening and semi-preparative
 
   

   
     


 
     
  

mode. The unique structure of this C12 ligand facilitates rapid equilibration between gradient runs.

PrincetonSPHER C30 is a 200 Å pore size material bonded with a distribution of long chain hydrocarbons that have
an average length of C30. This material has been found to successfully separate many isomers in the carotenoid family
of long chain molecules. It has also proved useful for the analysis of some larger molecules such as proteins
and peptides.

PrincetonSPHER PFP  

    
 
 
    
  


 
 
  
      
    
 

 
   
  


separations and is useful for differentiating halogen-containing compounds from their non-halogenated analogues.

PrincetonSPHER FO      


 


   
!    


   

 
 
"#$% & 
  
  


   
 '
   

  


their non-halogenated analogues.

Princeton ULTIMA bonded phases contain an amide polar embedded functionality, which deactivates neighbouring
free silanol groups and enhances the wettability of the bonded ligands. Enhanced ligand wettability makes the ULTIMA
C18 column especially suited for applications requiring the use of 100% aqueous mobile phases.

PharmaBOND columns are packed with 125 Å pore size 10 μm (or 5 μm for C18 phase) irregular silica particles.
C18, Phenyl, CN, Amino and Silica phases are available and are suitable for legacy and pharmacopoeia methods
specifying this phase type. They are equivalent to Waters μBondapak and are supplied with internal diameters of
3.9 and 4.6 mm.

12
 PRINCETON
Specifications of PrincetonSPHER HPLC Phases CHROMATOGRAPHY INC

P
at Poa S Sa
 Aa
C
ao o


P
 E
pp USP P
 Co
S e
e(
e 2/
e

PrincetonSPHER-60 Series
C18 3, 5, 10 60 500 23 Yes L1 01
C8 3, 5, 10 60 500 15 Yes L7 02
C6 3, 5, 10 60 500 10 Yes L15 03
C4 3, 5, 10 60 500 8 No L26 04
Phenyl 3, 5, 10 60 500 16 Yes L11 05
Diphenyl 5 60 500 10 Yes L11 51
PFP 3, 5, 10 60 500 12 Yes L43 06
CN 3, 5, 10 60 500 8 No L10 07
 3, 5, 10 60 500 6 No L8 08
 3, 5, 10 60 500 6 No L20 09
 5, 10 60 500 9 No L20 79
Silica 3, 5, 10 60 500 n/a n/a L3 10
PrincetonSPHER-100 Series
C18 3, 5, 10 100 325 19 Yes L1 01
C8 3, 5, 10 100 325 11 Yes L7 02
C6 3, 5, 10 100 325 8 Yes L15 03
C4 3, 5, 10 100 325 6 No L26 04
Phenyl 3, 5, 10 100 325 12 Yes L11 05
PFP 3, 5, 10 100 325 9 Yes L43 06
CN 3, 5, 10 100 325 6 No L10 07
 3, 5, 10 100 325 4 No L8 08
 3, 5, 10 100 325 4 No L20 09
Silica 3, 5, 10 100 325 n/a n/a L3 10
PrincetonSPHER-300 Series
C18 5, 10 300 100 8 Yes L1 01
C8 5, 10 300 100 5 Yes L7 02
C4 5, 10 300 100 3 No L26 04
Phenyl 5, 10 300 100 5 Yes L11 05
CN 5, 10 300 100 3 No L10 07
 5, 10 300 100 3 No L8 08
 5, 10 300 100 2 No L20 09
Silica 5, 10 300 100 n/a n/a L3 10
PrincetonSPHER-Phases
C30 3, 5, 10 200 200 19 No L62 74
HTS (C12) 3, 5, 10 60 500 16 Yes - 70
Cyclohexyl 3, 5, 10 60 500 12 Yes - 43
Fluorooctyl 5, 10 100 325 8 Yes - 42
Fluoropropyl 5, 10 100 325 5 No - 41
DEAP 5, 10 60 500 10.5 No - 75
ULTIMA Phases
C18 3, 5, 10 100 325 16 Yes L1 21
C8 3, 5, 10 100 325 13 Yes L7 22
Phenyl 3, 5, 10 100 325 12 Yes L11 23
PharmaBOND Phases
C18 5, 10 125 300 10 Yes L1 01
Phenyl 10 125 300 8 Yes L11 05
CN 10 125 300 6 No L10 07
Amino 10 125 300 4 No L8 08
Silica 10 125 300 n/a n/a L3 10

13
PRINCETON
CHROMATOGRAPHY INC

PrincetonSPHER HPLC Columns

Column Dimensions
Analytical HPLC columns can be supplied with lengths of 50, 75, 100, 150 and 250 mm and with i.d.s of 2.0, 4.0 and
4.6 mm.
Semi-preparative and Preparative HPLC columns can be supplied with lengths of 50, 100, 150 and 250 mm and with
i.d.s of 7.8, 10.0, 21.2, 30.0 and 50.0 mm.
Creating a Part Number
All Princeton Chromatography column part numbers are of the format XY-ABC
where X = column length in mm A = code for pore size
Y = code for column i.d. B = code for particle size

  
     
 
 
 

When creating a part number, please substitute the appropriate codes into this basic format.
e.g. for a 3 μm 150 x 4.6 mm i.d. PrincetonSPHER-100 C18 column, the part number is 150046-03301.

Column i.d. (mm) 2.0 3.0 4.0 4.6 7.8 10.0 21.2 30.0 50.0
Code 020 030 040 046 078 100 212 300 500

Pore Size (Å) 60 100 125 200 300 Particle Size (µm) 3 5 10
Code 01 03 05 07 08 Code 3 5 0

Princeton Guard Column System

We recommend that you use guard cartridges in order to extend the lifetime of your column, especially when
analyzing samples that are known to contain components which can irreversibly adsorb to the silica phase. The
cartridge should be replaced when the column pressure increases, or when the chromatography starts to degrade.
The PrincetonGUARD (Analytical and Preparative) column system (holder and cartridge) conveniently attaches to
any HPLC column. Princeton Analytical and Preparative guard cartridges (5/pack) can be prepared with the same lot
of packing material that is in the analytical or preparative column, if requested.
Part numbers for guard cartridges are created in the same way as for the analytical columns, by substituting values for
A, B and C from the tables above.

Princeton AnalyticalGUARD Princeton PreparativeGUARD

Part Number Part Number
10 x 4.0 mm cartridges (5/pk) 14104-ABC 10 x 10.0 mm cartridges (5/pk) 143010-ABC
10 x 2.0 mm cartridges (5/pk) 14102-ABC 10 x 21.2 mm cartridges (1/pk) 144021-ABC
Holder for 4.0 and 2.0 mm i.d. cartridges 1410 Holder for 10.0 mm i.d. cartridges 1430
Holder for 21.2 mm i.d. cartridges 1440

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14
 PRINCETON
CHROMATOGRAPHY INC

HPLC Applications
 Diuretics
Column: PrincetonSPHER C18, 100 Å, 5 μm
Catalog No: 150046-03501
3 Dimensions: 150 x 4.6 mm
Eluent: 60:40 A:B
A: 20 mM NH2H2
4, pH 3.2
4 B: Acetonitrile
Flow Rate: 1.5 ml/min
Temperature: 40°C
 Detection: UV at 280 nm
5 Injection: 10 μl (1 mg/ml sample)
1. Hydrochlorothiazide
2. Chlorthalidone
3. Diazoxide
4. Furosemide
0 2 4 6 8 10 12  
 
Minutes

Aromatic Acids 5
Column: PrincetonSPHER C18, 100 Å, 5 μm
Catalog No: 100046-03501
Dimensions: 100 x 4.6 mm
Eluent: 50:50 A:B
A: 0.1M H3
4, pH 2.0
B: Methanol
Flow Rate: 1.5 ml/min
Temperature: Ambient 
Detection: UV at 240 nm 
Injection: 5 μl (1 mg/ml sample)
1. p-Aminobenzoic acid 3
2. p-Hydroxybenzoic acid 4
3. o-Aminobenzoic acid
4. Benzyl alcohol
5. Salicylic acid
0 1 2 3 4 5 6
Minutes

Steroids
 Column: PrincetonSPHER C18, 100 Å, 5 μm
Catalog No: 250046-03501
Dimensions: 250 x 4.6 mm
Eluent: 66:34 A:B
A: Acetonitrile
B: Water
Flow Rate: 1.0 ml/min
 Temperature: 20°C
Detection: UV at 200 nm
Injection: 10 μl (1 mg/ml sample)
1 Ethinyl estradiol
2 Norethindrone

0 1 2 3 4 5 6
Minutes

15
PRINCETON
CHROMATOGRAPHY INC

HPLC Applications
1 Explosives
Column: PrincetonSPHER C18, 60 Å, 5 μm
Catalog No: 250046-01501
Dimensions: 250 x 4.6 mm
Eluent: 70:30 A:B
A: Acetonitrile
B: Water
Flow Rate: 1.5 ml/min
Temperature: 30°C
5 8 Detection: UV at 254 nm
3 4 6 Injection: 10 μl (1 mg/ml sample)
1. 2,4-DNT
2 7 2. 4,4’-DPA
3. 4N-DPA
4.

5. 2,4-DPA
9 6. 2,2’-DPA
7. 2,4-DPA
0
8. DPA
9. 2N-DPA
0 1 2 3 4 5 6 7 8 9 10 11
Minutes

Uracil and 5-Fluorouracil

1
Column: 
  
    
Catalog No: 250046-01509
Dimensions: 250 x 4.6 mm
Eluent: 80:20 A:B
A: Acetonitrile
B: Water
FlowRate: 1.0 ml/min
Temperature: 20°C 2
Detection: UV at 254 nm
Injection: 10 μl (1 mg/ml sample)
1. 5-Fluorouracil
2. Uracil
0

0 1 2 3 4 5 6 7
Minutes

1 Creatine and Creatinine

Column: 
      
Catalog No: 300039-05001
Dimensions: 300 x 3.9 mm
Eluent: 70:30 A:B*
A: 0.2% H3
4
B: Acetonitrile
* with 1.4 mg/ml dodecyl sulfate sodium
Flow Rate: 1.0 ml/min
Temperature: 20°C
Detection: UV at 210 nm
2 Injection: 5 μl (1 mg/ml sample)
1. Creatine
2. Creatinine
0 1 2 3 4 5 6 7 8 9 10 11 12 13 14
Minutes
16
 PRINCETON
CHROMATOGRAPHY INC

HPLC Applications
1
Taxol
Column: PrincetonSPHER PFP, 60 Å, 5 μm
Catalog No: 250046-01506
Dimensions: 250 x 4.6 mm
Eluent: 50:50 A:B
A: Acetonitrile
B: Water
Flow Rate: 1.0 ml/min
Temperature: 30°C
Detection: UV at 230 nm
Injection: 10 μl (1 mg/ml sample)
1. Taxol
0

0 1 2 3 4 5 6 7 8 9 10
Minutes

Vanillins 1
Column:

      
Catalog No: 150046-01542
Dimensions: 150 x 4.6 mm
Eluent: 30:70 A:B
A: Acetonitrile
B: 0.1% H3 4 2
Flow Rate: 1.0 ml/min
Temperature: 20°C
Detection: UV at 254 nm 3
Injection: 5 μl (1 mg/ml sample)
1. Vanillic acid
2. Vanillin
3. Ethyl vanillin
0

0 1 2 3 4 5 6 7
Minutes

2 Flavonoids
Column:

      
Catalog No. 250046-01542
Dimensions: 250 x 4.6 mm
Eluent: 60:40 A:B
A: 0.1% H3 4
B: Acetonitrile
Flow Rate: 1.0 ml/min
1 Temperature: 20°C
Detection: UV at 254 nm
Injection: 10 μl (1 mg/ml sample)
1. Quercetin
2. Naringenin

0 1 2 3 4 5 6 7
Minutes

17
PRINCETON
CHROMATOGRAPHY INC

Other Princeton Chromatography Services

Bulk Media
At Princeton Chromatography, it is our mission to provide our customers with a complete chromatography solution. To
that end, we offer the majority of our stationary phases in bulk form. Each SFC and HPLC stationary phase is available in
a variety of particle sizes, ranging from 10 μm to 20 μm for spherical and larger for irregular silica. We have the capacity


 
  

   


 
   
    
     
 

have been proven time and time again to be highly scalable and extremely reliable. All bulk media provided by Princeton
Chromatography are custom bonded on site and are subjected to extremely stringent quality standards.

Custom Column Packing

We are proud to offer custom column packing services at our facility in Cranbury, NJ. At Princeton Chromatography, we
have the capability to pack up to 50 mm i.d. columns. No job is too big or too small. With over 40 years of column packing
experience, our production team is highly skilled. We can provide one or two day turnaround times on smaller jobs. We
 
   


 
   

 

  



  
    

columns for SMB applications, with a heavy focus on repacking bulk chiral media (supplied by the customer). We have


  

  
 

  
     
   
 
 





about our column packing services.

Contract Purification


 

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Method Development
  
 
 

 
 


  
 
   
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selection assistance, to validation, Princeton Chromatography can provide solutions for all of your method development
needs. Please contact one of our chromatography experts for more information.

C()*+,* -./
Princeton Chromatography Inc.
T: 1-609-860-1803
Email: sales@pci-hplc.com

Alternatively Contact our Global Partner:

Hichrom Ltd,
T: +44 (0) 118 9303660
Email: technical@hichrom.co.uk

18
 PRINCETON
CHROMATOGRAPHY INC

OUR MISSION
Our mission at Princeton Chromatography, Inc. is to provide all our customers with the highest
quality HPLC and SFC solutions the market has to offer. With over 40 years of combined HPLC
experience and close to 15 years serving the SFC market, we are able to provide an unmatched
   ! " # $   $ ! " %# $& '
team of professional chromatographers is available to assist you not only in the selection and
purchase of your columns, but with long-term support. Here at Princeton Chromatography we
are constantly innovating and pushing the limits of HPLC and SFC. Quality. Choices. Flexibility.
Innovation. Support. Five words – one goal. The complete and total satisfaction of
our customers.

Quality Flexibility
Each and every column is tested and shipped With so many stationary phases and column
with an original chromatogram. All stationary dimensions to choose from, we can make your
phase media are bonded at our facility, so the scale up easy and worry free. From 2.0 mm
quality and reproducibility of each batch can screening columns for LC-MS to kilograms of bulk
be closely monitored. All columns are packed media, we are with you every step of the way.
and tested on-site by our team of production
specialists to ensure the highest level of Innovation
satisfaction for our customers. Chromatography technology is always
moving forward and the challenges facing our
Choices customers are constantly changing. We have
We offer a wide range of stationary phases. With the ability to react quickly to the changing
new innovative phases still being added periodically, environment and offer a wide range of products
we feel certain we can meet all of your HPLC and to meet all your needs.
SFC needs. All phases are available in a range of
particle sizes. From standard to highly specialized Support
custom requirements, nothing is out of reach. We With over 40 years of hands-on experience, we
also offer a complete array of column diameters
 
   
 
   
and lengths. Everything from 2.0 mm i.d. through problems. Our staff are eager to assist you.
to 50.0 mm i.d. Please let us know how we can be of service!

Princeton Chromatography Inc.

1206 Cranbury So. River Road, Cranbury, NJ 08512
T: 1-609-860-1803 • F: 1-609-860-1805 • Email: sales@pci-hplc.com • www.pci-hplc.com

Global Distribution Partner

Hichrom Ltd, 1 The Markham Centre, Station Road, Theale, Reading, Berkshire RG7 4PE UK
T: +44 (0) 118 9303660 • F: +44 (0) 118 9323484 • Email: sales@hichrom.co.uk • www.hichrom.co.uk