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RF Short Procedure Updated

The document provides instructions for performing an immunoturbidimetric test to measure RA factor levels. It details how to reconstitute and dilute the calibrator, the test programming parameters including wavelength, temperature, reagent and sample volumes, and delay and read times. It also lists the normal and linear ranges. The test procedure section shows the reagent and sample volumes used for the blank, calibrators and test samples.

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Dinesh Sreedharan
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17 views1 page

RF Short Procedure Updated

The document provides instructions for performing an immunoturbidimetric test to measure RA factor levels. It details how to reconstitute and dilute the calibrator, the test programming parameters including wavelength, temperature, reagent and sample volumes, and delay and read times. It also lists the normal and linear ranges. The test procedure section shows the reagent and sample volumes used for the blank, calibrators and test samples.

Uploaded by

Dinesh Sreedharan
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PDF, TXT or read online on Scribd
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RA FACTOR - Immunoturbidimetric Method

Reconstitute with 2mL of Distilled water


CALIBRATOR PREPARATION :
(Calibrator concentration is stated on vial)
Calibrator Dilution 1 2 3 4 5
Calibrator RF(µL) 10 25 50 75 100
Normal Saline(µL) 90 75 50 25 0
Factor 0.1 0.25 0.5 0.75 1

Note : Reagents is Ready to Use

TEST PROGRAMMING :
Fixed Time or Two
Mode of Reaction
Point Kinetic
Wavelength - Primary 650 nm(620 or 630)
Temperature 37°C
Working Reagent Vol 1000/ 500µL
Sample Volume 10/ 5 µL
Delay Time 10 seconds
Read Time 120 seconds
Reaction Direction Increasing
Blank Reagent
Unit IU/mL
Normal Range Upto 20 IU/mL
Linearity Up to 160 IU/mL

TEST PROCEDURE :
Blank Calibrator Test
Reagent1 (µL) 800 / 400 800 / 400 800 / 400
Reagent2 (µL) 200 / 100 200 / 100 200 / 100
DM water (µL) *10 / 5
Calibrator (µL) *10 /5
Sample/Control (µL) *10 / 5

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