CoolBox

Ice-Free Cooling and Freezing Systems

CoolBox systems are ice-free bench top cooling workstations that provide sample cooling or freezing without ice, electricity or batteries! CoolBox systems are versatile
® ®
and can accommodate a variety of sample formats and temperatures. The modular design enables the use of CoolRack and CoolSink thermo-conductive sample
modules to hold microfuge tubes, cryogenic vials, PCR tubes and plates, assay plates and more. The internal cooling or freezing cartridges provide hours of 0.5 to 4˚C
cooling or sample freezing. CoolBox systems may also be used with dry ice to create a compact, portable, snap-freezing workstation for bacteria, virus and proteins.

Problem: wet samples Solution: CoolBox

• Non-uniform ice contact • No wet ice contact
results in variable sample • Uniform well-to-well
temperature sample cooling
• Disorganized, wet • Samples are organized,
samples and labels secure and dry
• Shifting, sinking tubes; • Minimal risk of
contamination risk contamination
• Non-reproducible method • Reproducible method

Applications
Ice-Free Cooling and Freezing PCR Preparation Cell Cryopreservation Short Transport
Samples stay cold with a re-usable Keep PCR plates or strips and reagent Using a CoolBox system in the CoolBox systems provide optimal
cooling cartridge inside CoolBox tubes cold without ice in a CoolBox thawing process ensures proper cooling conditions when transporting
keeping samples cold (0.5 to 4˚C) for system. Samples remain cold (0.5 to temperature maintenance. Studies cold or frozen samples short
up to 16 hours. Ice-related problems 4˚C) with a reusable cooling cartridge have shown that one of the main distances. The reusable cooling or
such as unorganized, wet samples, and ice-related contamination risk factors in increasing survival and freezing cartridge ensures all samples
sample mis-identification and risk of from microbes and nucleases is proliferative capacity of stem cells is are kept at uniform temperature.
contamination are eliminated. Ideal minimized. Samples are maintained temperature, and how quickly they Ideal for transporting samples from
for enzyme and reagent cooling, or at uniform temperature, elminating are brought back from cryogenic building to building, or from clinic
protecting samples from temperature temperature variability. conditions to room temperature. to lab.
fluctuations in the freezer.

How It Works Performance

Choose from three different CoolBox

Lid systems. Each system consists of a
base, cooling cartridge/core and a lid.
CoolBox XT workstations also include
a magnetized collar. Each CoolBox
Collar system is designed to receive thermo-
conductive sample modules that sit
Cooling or Freezing directly on the cartridge/core keeping
Core (Pre-frozen in samples at desired temperature for
-20˚C freezer) hours. The cartridge/core is pre-frozen
in a -20˚C freezer.

Base CoolBox XT performance with XT Cooling Core. Samples stay cold for over
10 hours with lid open and over 16 hours with lid closed.
 CoolBox XT and CoolBox 2XT Workstations
Up to 16 Hours of Ice-Free Cooling or Freezing for Tubes and Plates

CoolBox XT and CoolBox 2XT workstations

keep tube or plate samples cold for over
16 hours with the lid on, and over 10 hours
with the lid off. Use optional XT Freezing
Core to maintain frozen samples for up
to 8 hours. Dry ice may be used in place
of the cores to create a compact snap CoolBox XT workstation CoolBox 2XT workstation
freezing workstation. Holds one sample module Holds two sample modules

CoolBox XT Performance Lid

Temperature Range Cooling Source Open Lid Closed Lid

0.5oC to 4oC XT Cooling Core Over 10 hours Over 16 hours Magnetized collar
-20 C to 0 C
o o
XT Freezing Core Over 5 hours Over 8 hours
-78oC 200ml Dry Ice* Over 4 hours Over 5 hours XT Cooling Core(s)
or XT Freezing
*Note: the thermo-conductive design of CoolRack and CoolSink sample modules ensures Core(s)(Pre-frozen in
uniform well-to-well temperature regardless of the consistency of the dry ice. -20˚C freezer)
Magnetized base

Ordering Information

Complete Workstations
Application Item No. Description and Contents For Use With Box Color
Enzyme, PCR reagent or other BCS-576 MicroTube 24 Workstation 24 microfuge tubes (1.5 or 2.0 mL)
biological specimen cooling or CoolBox XT System, SBS-compatible CoolRack XT M24,
snap-freezing one pack of 500 TruCool® 1.5 mL microfuge tubes.

Cell thawing, tissue culture, BCS-575 CryoTube 24 Workstation 24 cryogenic vials (1.0 to 2.0 mL), wells have “gripping” feature
bacteria/virus snap-freezing CoolBox XT System, SBS-compatible CoolRack XT CFT24, for one-hand tube opening
one pack of 50 TruCool® 2 mL internal-thread cryogenic vials

PCR/qPCR plate preparation BCS-570 PCR96 Workstation One 96-well PCR plate, 12 strip wells or 96 tubes
CoolBox XT System, SBS-compatible CoolRack XT PCR96,
one pack of 500 TruCool® 1.5 mL microfuge tubes.

PCR/qPCR strip well and BCS-572 PCR Strip Workstation 6 strip wells and 12 microfuge tubes (1.5 to 2.0 mL)
reagent tube preparation CoolBox XT System, SBS-compatible CoolRack XT M-PCR,
one pack of 500 TruCool® 1.5 mL microfuge tubes.

PCR/qPCR plate and reagent BCS-573 PCR Sample Preparation Workstation One 96-well PCR plate or strip wells or tubes and 24 microfuge
tube preparation CoolBox 2XT System, SBS-compatible CoolRack XT tubes (1.5 or 2.0 mL)
M24, SBS-compatible CoolRack XT PCR96, one pack of 500
TruCool® 1.5 mL microfuge tubes.

“Design Your Own” Workstation

CoolBox XT Workstation Sample Modules for CoolBox XT and CoolBox 2XT
Includes one (1) XT Cooling Core for 0.5 to 4˚C cooling. Holds one (1) Note: All XT modules are SBS-compatible and may be placed on robotic instruments for brief cooling outside of CoolBox.
sample module (Order separately from table on the right.) Item No. Description For Use With Dimensions (L x W x H)
Item No. Description Dimensions (L x W x H) BCS-535 CoolRack XT M24 24 x 1.5 mL microfuge tubes 12.8 x 8.5 x 3.8 cm
BCS-502 ● CoolBox XT, purple Internal: 14 x 9.4 x 9.9 cm* BCS-534 CoolRack XT CFT24 24 x 1.0 or 2.0 mL cryogenic vials 12.8 x 8.5 x 3.8 cm
BCS-502G ● CoolBox XT, green External: 20 x 16 x 15 cm BCS-529 CoolRack XT PCR96 one 96-well PCR plate, strip wells, tubes 12.8 x 8.5 x 2.5 cm
BCS-502O ● CoolBox XT, orange BCS-523 CoolRack XT M-PCR 12 x 1.5 or 2.0 mL microfuge tubes and 12.8 x 8.5 x 3.8 cm
BCS-502PK ● CoolBox XT, pink 6 strip wells
BCS-538 CoolRack XT PCR384 one 384-well plate 12.8 x 8.5 x 1.8 cm
BCS-536 CoolSink XT 96F one 96 or 384-well flat-bottom plate 12.8 x 8.5 x 1.7 cm
CoolBox 2XT Workstation BCS-537 CoolSink XT 96U one 96-well u-bottom plate 12.8 x 8.5 x 1.7 cm
Includes two (2) XT Cooling Cores for 0.5 to 4˚C cooling. Holds two (2)
sample modules (Order separately from table on the right.) Tall Sample Modules for CoolBox XT and CoolBox 2XT
Item No. Description Dimensions (L x W x H) Note: Tall modules require an XT Extension Collar. See next page for details.
BCS-503 ● CoolBox 2XT, purple Internal: 21 x 14.3 x 13.4 cm* Item No. Description For Use With Dimensions (L x W x H)
BCS-503G ● CoolBox 2XT, green External: 26.5 x 20 x 15 cm BCS-232 CoolRack L* 12 centrifuge tubes (15 mL), insulated 13.3 x 9.2 x 11 cm
BCS-503O ● CoolBox 2XT, orange BCS-153 CoolRack 15ml 9 centrifuge tubes (15 mL) 8.9 x 8.9 x 10.7 cm
BCS-503PK ● CoolBox 2XT, pink BCS-154 CoolRack 50ml 4 centrifuge tubes (50 mL) 8.9 x 8.9 x 10.7 cm
*Internal height of open space when core is in the base. BCS-235 CoolRack LV* 12 blood tubes (13x75mm), insulated 13.3 x 9.2 x 8.6 cm
BCS-157 CoolRack VS13 9 blood tubes (13x75 mm) 8.9 x 8.9 x 6.1 cm
BCS-155 CoolRack V13 9 blood tubes (13x100 mm) 8.9 x 8.9 x 8.4 cm
For additional sample modules visit www.biocision.com BCS-156 CoolRack V16 9 blood tubes (16x100 mm) 8.9 x 8.9 x 8.4 cm
*No XT Extension Collar necessary unless lid closing is required.
CoolBox XT and CoolBox 2XT Optional Accessories

CoolBox XT Extension Collar CoolBox 2XT Extension Collar XT Cooling Core XT Freezing Core
For use with CoolBox XT when using taller tube modules For use with CoolBox 2XT when using taller tube modules For 0.5 to 4˚C cooling For maintaining frozen samples <0˚C
Dimensions (L x W x H): Internal: 14 x 9.4 x 7 cm. Dimensions( L x W x H): Internal: 21 x 14.3 x 7 cm. Item No. Description Item No. Description
External: 20 x 16 x 7 cm. External: 26.5 x 20 x 7 cm. BCS-511 XT Cooling Core BCS-512 XT Freezing Core
Item No. Description Item No. Description
BCS-502C ● Extension Collar, purple BCS-503C ● Extension Collar, purple
BCS-502C-G ● Extension Collar, green BCS-503C-G ● Extension Collar, green
BCS-502C-O ● Extension Collar, orange BCS-503C-O ● Extension Collar, orange
BCS-502C-PK ● Extension Collar, pink BCS-503C-PK ● Extension Collar, pink

CoolBox MP Systems
Up to 10 Hours of Ice-Free Cooling for Tubes or Plates

CoolBox MP system keeps sample

tubes or plates cold (0.5 to 4.0˚C) for
approximately 10 hours with the lid Lid
closed, and approximately 4 hours with
the lid open. To extend the cooling
CoolRack or CoolSink
duration, keep an additional cooling sample module
cartridge in the freezer and replace the
cartridge as needed. Cooling Cartridge
(Pre-frozen in -20˚C
freezer)
CoolBox MP Performance CoolBox MP System
Temperature Range Cooling Source Open Lid Closed Lid Base
0.5˚C to 4˚C Cooling Cartridge Up to 4 hours Up to 10 hours
-78oC 200ml Dry Ice* Up to 4 hours Up to 5 hours

*Note: the thermo-conductive design of CoolRack and CoolSink sample modules ensures
uniform well-to-well temperature regardless of the consistency of the dry ice.

Ordering Information

Complete Systems
Application Item No. Description and Contents For Use With Box Color
PCR/qPCR plate preparation BCS-145 PCR96 CoolBox System one 96-well PCR plate or strip wells or tubes
CoolBox MP System, SBS-compatible CoolRack XT PCR96

PCR/qPCR 384-well plate preparation BCS-146 PCR384 CoolBox System one 384-well PCR plate
CoolBox MP System, SBS-compatible CoolRack XT PCR384

Tissue culture, cell staining, BCS-147 96F CoolBox System one 96-well or 384-well flat-bottom plate
other assays CoolBox MP System, SBS-compatible CoolSink XT 96F

Bradford protein, other colorimetric or BCS-148 96U CoolBox System one 96-well u-bottom plate
fluorescent assays CoolBox MP System, SBS-compatible CoolSink XT 96U

“Design Your Own” System

CoolBox MP System Sample Modules for CoolBox MP
Includes: CoolBox MP base and lid, one blue cooling cartridge. Item No. Description For Use With Dimensions (L x W x H)
Accomodates one sample module (Order separately from table on the right.) BCS-125 CoolRack M15 15 x 1.5 or 2.0 mL microfuge tubes 10.2 x 6.4 x 3.8 cm
Item No. Description Dimensions (L x W x H) BCS-127 CoolRack M15-PF (1.5ml) 15 conical 1.5 mL microfuge tubes 10.2 x 6.4 x 3.8 cm
BCS-144 ● CoolBox MP, purple Internal: 13.3 x 8.8 x 7.3 cm* BCS-535 CoolRack XT M24* 24 1.5 mL microfuge tubes 12.8 x 8.5 x 3.8 cm
BCS-144G ● CoolBox MP, green External: 20.3 x 15.25 x 15.25 cm BCS-126 CoolRack CF15 15 cryogenic vials 10.2 x 6.4 x 3.8 cm
BCS-144O ● CoolBox MP, orange BCS-534 CoolRack XT CFT24* 24 cryogenic vials 12.8 x 8.5 x 3.8 cm
BCS-144PK ● CoolBox MP, pink BCS-529 CoolRack XT PCR96* one 96-well PCR plate or 12 strip wells 12.8 x 8.5 x 2.5 cm
*Internal height of open space when core is in the base. BCS-523 CoolRack XT M-PCR* 12 x 1.5 or 2.0 mL microfuge tubes and 12.8 x 8.5 x 3.8 cm
6 strip wells
CoolBox MP Optional Accessories BCS-538 CoolRack XT PCR384* one 384-well PCR plate 12.8 x 8.5 x 1.8 cm
BCS-536 CoolSink XT 96F* one 96-well or 384-well flat-bottom plate 12.8 x 8.5 x 1.7 cm
Cooling Cartridge BCS-537 CoolSink XT 96U* one 96-well u-bottom plate 12.8 x 8.5 x 1.7 cm
Item No. Description BCS-149 CoolRack 96x1ml 1.4 mL 2D storage tubes 13.2 x 8.9 x 4.3 cm
BCS-152 Cooling cartridge, blue, 1 per pack BCS-231 CoolRack 96x0.5ml 0.5 mL 2D storage tubes 13.2 x 8.9 x 3.6 cm
*All XT modules are SBS-compatible and may be placed on robotic instruments for brief cooling outside of CoolBox.
 CoolBox 30 Systems
Up to 10 hours of Ice-Free Cooling or Freezing for Tubes

CoolBox 30 System keeps sample tubes Lid

cold (0.5 to 4.0˚C) for approximately 4
to 10 hours. Use the optional Freezing
Cartridge to maintain frozen samples CoolRack module
below 0˚C for approximately 3 to 6 hours.
Cooling Cartridge
(Pre-frozen in -20˚C
Freezer)
Base
CoolBox 30 Performance CoolBox 30 System
Temperature Range Cooling Source Open Lid Closed Lid
0.5oC to 4oC Cooling Cartridge Up to 4 hours Up to 10 hours
-20oC to 0oC Freezing Cartridge Up to 3 hours Up to 6 hours
-78oC 200ml Dry Ice* Up to 4 hours Up to 5 hours

*Note: the thermo-conductive design of CoolRack sample modules ensures

uniform well-to-well temperature regardless of the consistency of the dry ice.

Ordering Information

Complete Systems
Application Item No. Description and Contents For Use With Box Color
Enzyme and PCR reagent benchtop cooling BCS-133 M30 CoolBox System 30 x 1.5 mL or 2 mL microfuge tubes
or storage CoolBox 30 System, CoolRack M30

Enzyme and PCR reagent benchtop cooling or BCS-134 M30-PF CoolBox System 30 x 1.5 mL or 2 mL conical microfuge tubes
storage (conical 1.5 mL tubes only) CoolBox 30 System, CoolRack M30-PF

Cell thawing, tissue culture, bacteria/virus BCS-166 CFT30 CoolBox System 30 x 1 mL or 2 mL cryogenic vials
snap-freezing CoolBox 30 System, CoolRack CFT30

“Design Your Own” System

CoolBox 30 System Sample Modules for CoolBox 30
Includes: CoolBox 30 base and lid, blue cooling cartridge, Accomodates Item No. Description For Use With Dimensions (L x W x H)
15-well or 30-well CoolRack tube modules (Order separately from table BCS-125 CoolRack M15 15 x 1.5 mL or 2.0 mL microfuge tubes 10.2 x 6.4 x 3.8 cm
on the right.)
BCS-127 CoolRack M15-PF 15 x conical 1.5 mL microfuge tubes 10.2 x 6.4 x 3.8 cm
Item No. Description Dimensions (L x W x H) BCS-108 CoolRack M30 30 x 1.5 mL or 2.0 mL microfuge tubes 12 x 10.2 x 3.8 cm
BCS-130 ● CoolBox 30, purple Internal: 12 x 10.4 x 7.9 cm* BCS-128 CoolRack M30-PF 30 x conical 1.5 mL microfuge tubes 12 x 10.2 x 3.8 cm
BCS-130G ● CoolBox 30, green External: 16.5 x 15.25 x 12.7 cm
BCS-137 CoolRack M30-PF (500 ul) 30 x conical 500ul tubes 12 x 10.2 x 3.8 cm
BCS-130O ● CoolBox 30, orange BCS-126 CoolRack CF15 15 x 1.5 mL or 2.0 mL cryogenic vials or 10.2 x 6.4 x 3.8 cm
BCS-130PK ● CoolBox 30, pink 12x75 FACS tubes
*Internal height of open space when core is in the base.
BCS-138 CoolRack CFT30* 30 x 1.5 mL or 2.0 mL cryogenic vials or 12 x 10.2 x 3.8 cm
12x75 FACS tubes
CoolBox 30 Optional Accessories * CoolRack CFT30 has “gripping” wells for one-hand cryogenic vial opening/closing

Cooling Cartridge Freezing Cartridge

For 0.5 to 4˚C cooling For maintaining frozen samples <0˚C
Item No. Description Item No. Description
BCS-132 Cooling cartridge, blue, 3 per pack BCS-131 Freezing cartridge, green, 3 per pack

BioCision, LLC 12 E. Sir Francis Drake Boulevard, Unit B, Larkspur, CA 94939 USA sales@biocision.com www.biocision.com
© 2014. BioCision, LLC.  All rights reserved. Patents pending. BioCision, CoolBox, CoolRack, CoolSink, TruCool, Standardizing Samples designations are trademarks owned by BioCision, LLC