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Pickering Laboratories Product Catalog

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1
 Pickering Laboratories Product Catalog

As we approach our 4Oth year, we

celebrate the introduction of the

Onyx PCX!
It’s the next generation of HPLC post-column derivatization instruments, resulting
from Pickering Laboratories’ decades of experience in post-column instrument
manufacturing. Previously, the Pinnacle PCX set the benchmark for innovative
design as the top-of-the-line PCX model. Now, it is surpassed by the Onyx PCX as
the best post-column instrument available on the market.

Pickering Laboratories still offers the only instrumentation optimized for the analysis
of Amino Acids, Carbamates, Glyphosate, Mycotoxins, Antibiotics and many other
post-column applications. Each component is specifically designed to enhance
sensitivity and selectivity. Only Pickering Laboratories provides complete application
support, including chemicals, columns, methods and post-column systems. Because
each part of the method is designed to work together, Pickering Laboratories
can offer the extraordinary promise that the analysis is guaranteed to work for
the intended application. The Onyx PCX reflects the ease of use, reliability and
ruggedness customers have come to expect from Pickering Laboratories.

2
Pickering Laboratories Product Catalog

Table of Contents

Introduction Eluants 55 Aminoglycoside Antibiotics

55 Multi-Residue Mycotoxins
4 Customer Service 44 Sodium-based Solutions for
55 Aflatoxins
4 Place an Order Amino Acid Analysis
55 Polyether Antibiotics
4 Factory Repairs 44 Lithium-based Solutions for
5 Limited Warranty Amino Acid Analysis Replacement Components,
6 Instrument Service Plans 44 Glyphosate Herbicide Analysis Fittings & Tubing
44 Potassium-based Solutions for
Instruments & Hardware ALKION™ Column for Aminoglycoside
56 Onyx PCX Flow Diagram–
Two-Pump System
7  nyx PCX Derivatization Instrument
O Antibiotics & Streptomycin
57 Onyx PCX Flow Diagram–
11 Vector PCX Derivatization Instrument 44 Biogenic Amines / Polyamines Analysis
One-Pump System
13 UVE Photochemical Reactor 44 Multi-Residue Mycotoxin Analysis
58 Vector PCX Flow Diagram
Chromatograms, Test Mixtures & Standards 59 Onyx PCX Reagent Pump and
Columns & Guards 45 Calibration Standards and Test Mixtures
Valve Components
59 Pinnacle PCX Reagent Pump and
15 Chromatograms, Columns & Guards
16 Cation-exchange Columns
Clean-up Columns Valve Components
47 Immunoaffinity Sample 59 Vector PCX Reagent Pump Components
16 Reversed-Phase Columns
Clean-up Columns 59 Onyx PCX Replacement Reactors
17 Cation-exchange GARD™ Column
50 DONeX ™ 60 Pinnacle PCX Replacement Reactors
Protection System
51 Glyphosate and AMPA Sample 60 Vector PCX Substitute Reactor
17 Lithium Amino Acids Columns
Clean-up Cartridges Volumes or Replacement Reactors
23 Sodium Amino Acids Columns
60 Reagent Reservoirs & Cap Assemblies
27 Carbamate Pesticides Columns
Application Kits 60 Pressure Regulators, Valves & Gauges
31 Glyphosate Herbicide Analysis Column
52 30-min High-Efficiency Collagen 61 Flow Restrictors
32 Alkion™ Column
Hydrolysate 61 Filters & Frits
36 Mycotox™ Column
52 30-min High-Efficiency Protein 61 Tees & Unions
37 Polyether Antibiotics Columns
Hydrolysate 61 Tubings, Plastic
Reagents, Diluents & Sample 52 30-min High-Efficiency Oxidized 61 Fittings, Plastic
61 Fittings, Type 316 Stainless Steel
Preparation Solutions Feed Hydrolysate
52 Standard 60-min Protein Hydrolysate
38 TRIONE Ninhydrin Reagent Product Testing Solutions
52 High-Efficiency 55-min Protein
39 Thiofluor 62 Artificial Perspiration / Sebum
Hydrolysate
39 o-Phthaladehyde (OPA) 66 Artificial Saliva
53 High-Efficiency 55-min
39 Vanillin 67 Artificial Urine
Collagen Hydrolysate
40 Naphthalene-2,3-Dicarboxaldehyde 67 Simulated Lung Fluid
53 60-min Oxidized Feed Hydrolysate
40 p-Dimethylaminobenzaldehyde (DMAB) 67 Artificial Cerumen "Earwax"
53 70-min High-Efficiency Physiologic
40 o-Phthalaldehyde (OPA) Diluents 67 Substitute Ocean Water
Fluid/Native Sample
(CB910, GA104, OD104) 68 Synthetic Hard Water
53 Standard 185-min Physiologic
41 Hydrolysis Reagent for Carbamate
Pesticide Analysis CB130
Fluid/Native Sample Terms & Conditions
53 High-Efficiency 120-min Physiologic
41 Hydrolysis Reagent for Carbamate 68 General Terms & Conditions
Fluid/Native Sample
Pesticide Analysis CB130.2
41 Hypochlorite Diluent for Glyphosate
54 Temperature Gradient, Physiologic Distributors
Fluid/Native Sample Kit
Herbicide Analysis GA116 69 Distributors
54 Carbamate Pesticides,
42 Samples and Standards Diluents (Li220,
23+ Compounds
Na220)
54 Carbamate Pesticides, EPA
42 Solutions for Sample Preparation for
Method 531.1
Amino Acid Analysis (SERAPREP™,
54 Carbamate Pesticides, AOAC
URIPREP™)
Method 531.2
43 ChlorAC™ Buffer
54 Carbamate Pesticides, AOAC
43 Restore™
Method 985.23
55 Glyphosate Herbicide, EPA Method 547,
AOAC Method 991.08
55 Biogenic Amines/Polyamines

3
 Pickering Laboratories Product Catalog

Introduction

Customer Service will be operating. We will advise please call Technical Support early
& Technical Support you if any modifications to the to schedule our visit to your site.
HPLC are necessary. This is especially important if you
Pickering Laboratories has earned
purchase installation through
the reputation for strong Technical Terms a distributor or HPLC Company
Support in HPLC post-column
• No minimum order required because we need to hear all the
technology: chemistry, columns
• Net 30 days for established details directly from you.
and derivatization instruments.
We start with assisting our accounts, or prepayment in U.S. Individually tailored courses
customers in identifying the best dollars, or Letter-of-Credit are also given at our facility in
HPLC configuration required • We accept MasterCard & VISA, Mountain View, California. Please
for a successful integration of Discover Card or American telephone or e-mail with your
our HPLC post-column system. Express requirements and we will advise
Then we continue our support cost and schedule.
• Prices are Ex-Works Seller’s
through and beyond the warranty
Factory, Mountain View, California Product Returns
period to ensure our customers’
chromatograms reflect current U.S. Destinations
Products can be returned if
standards of quality and unopened. A restocking fee of 15 %
Freight charges are prepaid and of list price will be applied. Before
reproducibility.
added to the invoice. Shipments returning a product for any
Contact us are via UPS-Ground unless reason, please:
otherwise specified.
For Customer Service and • Phone or e-mail requesting a
Technical Support contact: Destinations Outside of the U.S. Returned Goods Authorization
Freight charges are collect. Unless Number.
Support@pickeringlabs.com
800-654-3330 / 650-694-6700 otherwise specified, shipments • Package the product so that it
overseas will be via customer’s will arrive undamaged. Use the
Place an Order freight forwarder. original packing if possible. If
Prices and specifications are sub- in doubt, please call. Pickering
Please contact us directly to
Laboratories cannot assume
place an order: ject to change without prior notice.
responsibility for goods which
Orders@pickeringlabs.com Establish Good Standing arrive damaged in shipment.
800-654-3330 / 650-694-6700
New Accounts: Before a credit-line
Factory Repairs
Pickering Laboratories, Inc. has been established, new account
1280 Space Park Way orders must be pre-paid. Freight Pickering Laboratories offers
Mountain View, CA 94043 charges are collect. factory repair for its instruments
(Onyx PCX, Pinnacle PCX and
When ordering, please provide Vector PCX only). We will re-build
the following:
Installation & Training
or replace components as
We encourage you to take advan-
• Complete shipping and billing necessary in our judgment, then
tage of Pickering Laboratories’
addresses test the instrument to make
individualized two or three-day
• Catalog number and quantity certain that the original problem
installation and training. For an
of each item has been solved.
all-inclusive fee we can provide
• Brief product description, as post-column system installation For repairs, we will charge for
found in Catalog and training at your site by an ex- shipping, parts and labor (current
perienced chemist from Pickering shop rate).
• Purchase order number (and
Laboratories or it’s representatives.
contract number, if applicable) What is Required
The training includes instrument
• Name and telephone number operation, routine maintenance Contact support to obtain a Return
of person calling and troubleshooting, and chro- Goods Authorization Number
• Special instructions, e.g., package matogram interpretation. (RGA) and a Health and Safety
markings, mode of transit if other Declaration form. When the instru-
Some of our authorized distribu-
than UPS ment is returned it must have the
tors offer their own training and
RGA noted on the outside of the
• If ordering a complete post- installation services.
box and a completed Health
column system, please specify When purchasing installation and and Safety Declaration, with
the brand of HPLC with which it training by Pickering Laboratories, the instrument.

4
Pickering Laboratories Product Catalog

Introduction

What is Done Field Service Analytical Columns

Upon arrival, we evaluate your sys- Because of our limited personnel, Pickering’s analytical columns are
tem to generate an estimated cost we prefer to resolve problems with- warranted for 90 days to be free of
for repairs. Once we have your ap- out site visits, but when necessary defects in materials and workman-
proval, we complete the repair. We we will schedule a site visit with ship under normal installation, use,
will replace damaged parts, pres- you. Please call for a quotation. and maintenance, for the warranted
sure test the instrument, and then On-site service entails an initial time beginning f rom the date
test the instrument to confirm the evaluation on the first day, with of delivery to the customer. Pickering
system meets the products original repairs potentially extending to the Laboratories will replace the an-
manufacturer’s specification. second day to allow for shipment alytical column under warranty
of parts. The shipment of parts will if found defective in material or
What you get workmanship. However, the warran-
be included in the invoice.
The minimum parts replaced are: ty is void if the analytical column
On-site repair costs will include
Restrictor(s)*, Seal(s) and Filters. was damaged due to customer’s
travel time, air fare, hotel, car
If we test the instrument to de- misuse.
rentals, expenses, plus parts
termine that it meets the original
and labor. How to Obtain Warranty Service
manufacturer’s specification, we
will warranty the instrument for If there is a problem with your
Limited Warranty instrument or analytical column
1 year on all the parts that were
replaced. If you request that we do Instruments within the warranty period, im-
not complete this test, we will not Instruments are warranted to be mediately notify Pickering or your
warranty our work. free of defects in material and local authorized representative.
workmanship under normal instal- If the instrument or analytical
lation, use, and maintenance for a column was not purchased directly
period of one year from the date of from Pickering, please contact the
delivery to the customer. Pickering vendor where it was purchased.
will replace or repair, without cost, Any instrument, part of the in-
any defective items. Expendable strument, or analytical column
items such as check valves, pistons, returned to Pickering for examina-
piston seals, and filters are exclud- tion or repair shall have Pickering’s
ed from this warranty. In addition, prior approval (call for a Returned
physical damage, poor-quality Goods Authorization Number)
reagent- and sample-induced and be shipped prepaid by the
damage, and instrument damage customer. Return transportation
due to customer’s misuse are not will be at Pickering’s expense if the
covered by this warranty. instrument, part of the instrument,
or analytical column is found to be
defective and under warranty.

* Vector systems only

5
 Pickering Laboratories Product Catalog

Introduction

Health & Safety Declaration Instrument & Service Plans Benefits of Service
Instruments and columns must We encourage you to consider Agreements
be decontaminated and cleaned including a Pickering Laboratories • Free software upgrades
prior to returning to Pickering. To Service Plan to match the quality • Installation of all required
protect and ensure the safety of and reliability you have come to performance upgrades
Pickering Laboratories personnel, expect from Pickering Laboratories.
customer must properly flush These offerings provide factory • PM/Service performed by
instrument of any and all hazards trained engineers experienced Pickering Laboratories trained
(e.g. reactive chemicals, blood in HPLC and post-column tech- service specialists
borne pathogens). Please contact nologies to service your Pickering • Fixed annual cost – no Surprises
Pickering Laboratories to obtain Laboratories post-column derivat- • Unlimited technical/analytical
a Health and Safety Declaration ization instruments. Each plan has support
Form (B-0107) describing return the features and benefits most
• Multi-unit service
instructions and customer requested by customers to offer
discounts available
responsibilities. Failure to do so the best value and effectiveness for
will result in delay of service and/or today’s busy laboratories.
additional charges. When you sign-up for the Pickering
Laboratories Service Plans, you
are assuring the continued quality
and reliability of your post-column
instrument as well as your analysis.

Comparison of Service Plans

Features Warranty Upgrade** Depot Service Elite Service*

Monday - Friday Monday - Friday Monday - Friday

Telephone support to isolate and
8 AM - 5 PM 8 AM - 5 PM 8 AM - 5 PM
resolve technical problems
Excluding Holidays Excluding Holidays Excluding Holidays

Recertification Included Included Included

Site Visits Two (including PM) At prevailing rates Two (including PM)

Preventative Maintenance One One One

Freight Charges Included Included Included

Parts and Labor Included Included Included

Service Plans offered for the Pickering Laboratories Onyx PCX, Pinnacle PCX and Vector PCX post-column derivatization
instruments. Pre-service inspection is required for instruments that have an expired manufacturers warranty or
service contract.
*The Elite Service plan provides a total of two service calls that will cover parts, labor and travel for the period of one year.
Included is unlimited telephone support from Pickering Laboratories Technical Service Engineers and Applications Specialists.
**Applies to new systems only.

For more information and quotations:

Please contact Pickering Laboratories’ Technical Support
at support@pickeringlabs.com or 800-654-3330 / 650-694-6700.

6
Pickering Laboratories Product Catalog

Instruments & Hardware

Onyx PCX

Introducing the Onyx PCX Laboratories offers complete • Quick-change reactor cartridges
Pickering Laboratories' newest application support, including make application switching
post-column instrument, the chemicals, columns, methods easy and replacements fast and
Onyx PCX, is part of our integrated and post-column systems. inexpensive.
family of instruments, chemistry Because each part of the method • The column oven utilizes
and support. is designed to work together, circulating air for consistency of
Pickering Laboratories can offer heating and quick cooling within
Meet the Onyx PCX the extraordinary promise that 1 °C of setpoint.
the analysis is guaranteed to work
It’s the next generation of HPLC • Inert flow paths extend system
for the intended application. The
post-column derivatization life and reduce maintenance.
Onyx PCX reflects the ease of
instruments resulting from • The PCX control software allows
use, reliability and ruggedness
Pickering Laboratories’ nearly 40 for precise control of the reagent
customers have come to expect
years of experience in post-column delivery and conservation.
from Pickering Laboratories.
instrument manufacturing.
Previously, the Pinnacle PCX set • Pre-programmed testing and
Accuracy, Durability, Speed maintenance procedures take
the benchmark for innovative
and Convenience the guess work out of
design as the top-of-the-line PCX
• Instrument layout is focused on instrument care.
model. Now, it is surpassed by
the Onyx PCX as the best post- ease of use, quick monitoring • Column oven temperature
column instrument available on and rapid service. gradient programming improves
the market. • The electronic syringe pump separation and run times. Only
provides true pulse-free flow Pickering Laboratories builds
Pickering Laboratories still
for superior sensitivity and post-column systems with
offers the only instrumentation this feature.
consistency. The pump cylinder
optimized for the analysis of
and head are made from a • Works with any HPLC
Amino Acids, Carbamates,
single piece of inert ceramic for instrument, from any
Glyphosate, Mycotoxins, Antibiotics
durability and non-reactivity. manufacturer.
and many other applications.
Each component is specifically • Electronic valves eliminate
designed to enhance sensitivity troublesome check valves and
and selectivity. Only Pickering allow automated pump flushing.

7
 Pickering Laboratories Product Catalog

Instruments & Hardware

Onyx PCX
Continued

Features and Benefits capability helps reduce Amino Acids Analysis run
• All PCX components specifically designed times. Even more significant is the ability to resolve
for post-column derivatization coelutions: consider such metabolic markers as
Alloisoleucine (MSUD) and Argininosuccinic acid
• Programmable temperature gradient column (ASA). Under standard isothermal conditions these
oven for faster Amino Acids Analysis run times amino acids coelute with Cystathionine and Isoleucine
• Column oven is oriented for easy column switching respectively, but both are resolved using a targeted
and features improved tubing guards temperature gradient program.
• Electronic pulse-free syringe pump provides The ability to accomplish this derives from the
greater sensitivity multiple retention mechanisms of the gel-type resins
• Automatic piston wash and programmable employed in ion-exchange, enabling all the amino
system flush acids to appear in the same chromatogram. The exact
position of each amino acid is influenced by an array
• Full-size removable reagent tray provides
of mechanisms including partitioning, adsorption,
convenience and meets secondary containment
requirements charge exclusion, etc. So even though two amino
acids might coelute, their proximity is incidental. And
• Electronic valves have no expensive check since retention processes are affected differently by
valves to service and replace changes in pH, salt concentration and temperature,
• Quick change reactor cartridges enable fast all these parameters have significant influence on
application switching and easy cartridge selectivity.
replacement
• Inert flow path protects from metal contamination PCX Control Software
and extends system life while reducing maintenance The Onyx PCX is controlled by PCX Control Software,
compatible with Windows 7 or newer Windows
• Color LCD display provides for continuous system
operating systems. Using the same computer as the
monitoring and critical message alerts
HPLC, the PCX Control Software interfaces easily with
• All fluidics located on front panel for instant access Agilent ChemStation or communicates with any other
and easy leak checks, drip tray included modern HPLC using a relay connection.
• Improved pump access for expedient maintenance The computer physically connects to the Onyx PCX
• Integrated gas manifold allows for easy set-up and unit through a USB cable. After an easy installation
facilitates reagent preparation and preservation and configuration, the software runs in a window or as
• PCX control software runs from Windows PC for easy an icon on the computer desktop. The main software
operation and reagent conservation between runs display matches the instrument’s digital LCD display,
where all PCX functions of temperature, flow rate and
• Software stores methods and sequences, allowing
system status are displayed in real time. This allows
for flexible application setup and switching
for monitoring and control of the PCX and HPLC from
• Seamless application migration from Pinnacle PCX one computer.
to Onyx PCX, including method transfers
• Log files collect continuous data, from system
status to error messages
• Pickering Support Team available for rapid log file
interpretation and troubleshooting assistance
• Serviceability simplified with removable instrument
side panels and isolated electronics
• Field calibration now available by trained
Pickering Laboratories service engineers to
support recertification

Programmable Temperature
Gradient: Amino Acids Analysis
The Onyx PCX provides a unique opportunity to
combine the eluant gradient capabilities of modern
HPLC instruments with programmable column
temperature gradients. As might be expected, this

8
Pickering Laboratories Product Catalog

Instruments & Hardware

Onyx PCX
Continued

Methods are managed within the Column Heater • Refill cycle of 60 seconds or less
PCX Control Software and can The column heater utilizes recircu- • Automatic piston wash
be created, edited and saved to lating airflow technology to provide
create a library for all application • Automatic reagent flush cycle
quick and uniform column heating.
parameters. A sequence table is • No instrument check valves
Fast column cooling is assisted by
used to schedule multiple runs the introduction of a fresh air flow Reactor
of the same or different methods into the chamber. The temperature
in a series. At the end of the • Heated reactor temperature from
range holds within 1 °C resolution
sequence a full system flush can be 5 °C above ambient to 130 °C
from 5 °C above ambient room
programmed. System and pump maximum setpoint
temperature to 75 °C maximum
performance can be evaluated in setpoint. The temperatures can • Stability +/- 0.5 °C
the maintenance menu, using a be programmed for a gradient • Accuracy +/- 1 °C
pump pressure test and system with as many steps as required for
pressure test. An instrument log • Thermal safety switch limits
fine-tuning an analysis. temperature to prevent damage
file continually records system
status and error messages for Specifications • Easy replacement coil cartridges
later reference and can be sent • Range of reactor dwell volumes
to Pickering Support for remote Dimensions
available, from 0.1 mL to 3 mL
diagnostics. • 22.4 H x 12.0 W x 19.15 D inches
• Reaction coil withstands up to
(56.9 x 30.5 x 48.6 cm), instrument
Electronic Syringe Pumps 42 bar (600 psi) inlet pressure
with doors closed
and Valves at 130 °C
Weight
The syringe pump’s cylinder and Column Heater
head are made from a single piece • 60 lbs or 27 kg for
• Heater accepts 6 or 8 mm OD
of 99.9 % Alumina for ruggedness dual-pump systems
(0.25 or 0.31 inch) x 50-250 mm
and nonreactivity. The piston • 50 lbs or 23 kg for in length Column and guard
surface is made from PEEK® with single-pump systems
• Temperature holds within
an inert O-ring seal. The piston seal
Electrical ± 1 °C resolution from 5 °C above
is protected by an automatic piston
• Factory configured as either 100- ambient to 75 °C maximum
wash system that provides long
120 VAC, 50/60 Hz, 1.7 A, 200 W or setpoint
seal life. The programmable flow
rates range from 50 µL to 1500 µL/ 200-240 VAC, 50/60 Hz, 0.8 A, 200 W • Programmable temperature
minute with a refill cycle of under • Mains voltage ± 10 % of nominal gradient
60 seconds. The electronic valve • Easy access to column
• Current 5 A maximum
utilizes PEEK® and Teflon® with a compartment and improved
port layout that eliminates cross • Installation (over voltage) category tubing guards
contamination. II, pollution degree 2
• Thermal safety switch limits
• Fuses, 2 ea., 5 mm x 20 mm, 6.3 A, temperature to prevent damage
Reactor 250 VAC, time lag
The reactor is designed for quick Instrument Package and Flow Path
Environmental
heating and easy switching • Advanced fluidics valve
between applications. The heating • Indoor use only management system
and control electronics are in the • Altitude up to 6500 ft (1981 m) • Completely inert flow path
base unit of the reactor while • Ambient temperature 15 ° - 29 °C
the coil volumes are inserted • Easy access to internal
with a ‘quick-change’ cartridge • Relative humidity up to 80 % components
on the front fluidics panel. The at 31 °C • Standard fittings
temperature range holds within Reagent Pumps • Side panels remove easily
1 °C resolution from 5 °C above for service
• True pulse-free syringe pump
ambient room temperature to
with single piece ceramic barrels • Integrated reagent reservoir
130 °C maximum setpoint.
• Maximum operating pressure tray compliant with secondary
35 bar (500 psi) containment requirements
• Programmable flow rates of
50 μL to 1500 μL/minute

9
 Pickering Laboratories Product Catalog

Instruments & Hardware

Onyx PCX
Continued

Display • Safety pressure-relief valve opens pre-calibrated relief valve opens

• LCD display, color 800 x 480 at 0.7 bar (10 psi) at 35 bar (500 psi) to prevent
pixels, 153 x 85 mm viewing area • Manifold with anti-siphon valves rupture of the post-column
and two 1/4-28 fittings reactor tubing in the event of
• Real time temperature, pressure
down-stream blockage
and critical system alerts shown
Safeguards • Back-pressure regulator applies
• Intuitive system status icons
• In-line check valve prevents 7 bar (100 psi) to the detector flow
Gas Pressure Manifold and Regulator reagent back flow into the cell outlet (waste line) to prevent
column when HPLC pressure detector noise and precipitation
• Panel-mounted gas manifold
drops due to out-gassing or boiling
• Regulator maintains 0.3 bar
• Replaceable reagent filters to EMC Compliance
(3-5 psi) on reagent reservoirs
prevent reactor fouling
with 3-5 bar (45-75 psi) source • Onyx PCX complies with EN 61326-1
pressure • Post-column system over-
pressure protection from

Onyx PCX Derivatization Instruments

Catalog No. Description

1155-1011 Onyx, Single Pump, 0.15 mL, 120 V (Primary Amino Acids w/OPA, Aminoglycoside Antibiotics, Biogenic Amines, Sulfonamide Artificial Sweetener)

1155-1012 Onyx, Single Pump, 0.15 mL, 240 V (Primary Amino Acids w/OPA, Aminoglycoside Antibiotics, Biogenic Amines, Sulfonamide Artificial Sweetener)

1155-1021 Onyx, Single Pump, 0.5 mL, 120 V (Primary and Secondary Amino Acids w/TRIONE®, Bromate, Formaldehyde, B Vitamins, Theanine)

1155-1022 Onyx, Single Pump, 0.5 mL, 240 V (Primary and Secondary Amino Acids w/TRIONE®, Bromate, Formaldehyde, B Vitamins, Theanine)

1155-1031 Onyx, Single Pump, Knitted 1.4 mL, 120 V (Mycotoxins, Aflatoxins, Fumonisins, Sulfonamide Drugs)

1155-1032 Onyx, Single Pump, Knitted 1.4 mL, 240 V (Mycotoxins, Aflatoxins, Fumonisins, Sulfonamide Drugs)

1155-1051 Onyx, Dual Pump, 0.5 mL, 120 V (Carbamate, Glyphosate, Streptomycin, Primary and Secondary Amino Acids w/OPA)

1155-1052 Onyx, Dual Pump, 0.5 mL, 240 V (Carbamate, Glyphosate, Streptomycin, Primary and Secondary Amino Acids w/OPA)

1155-1061 Onyx, Dual Pump, Knitted 1.0 mL, 120 V (Paralytic Shellfish Toxins)

1155-1062 Onyx, Dual Pump, Knitted 1.0 mL, 240 V (Paralytic Shellfish Toxins)

1155-1071 Onyx, Dual Pump, Knitted 1.2 & 1.6 mL, 120 V (Tricothescene)

1155-1072 Onyx, Dual Pump, Knitted 1.2 & 1.6 mL, 240 V (Tricothescene)

1155-1081 Onyx, Dual Pump, Reverse Plumbing, Knitted 1.4 mL, 120 V (Polyether Antibiotics)

1155-1082 Onyx, Dual Pump, Reverse Plumbing, Knitted 1.4 mL, 240 V (Polyether Antibiotics)

1155-1101 Onyx, Single Pump, Knitted 3.5 mL, 120 V (Voglibose)

1155-1102 Onyx, Single Pump, Knitted 3.5 mL, 240 V (Voglibose)

1155-1103 Onyx, Single Pump, Dead-head Pressure Transducer, Knitted 1.0 mL, 120 V (Chromium VI)

1155-1104 Onyx, Single Pump, Dead-head Pressure Transducer, Knitted 1.0 mL, 240 V (Chromium VI)

1155-1105 Onyx, Single Pump, Dead-head Pressure Transducer, Knitted 2.0 mL, 120 V (Alprostadil)

1155-1106 Onyx, Single Pump, Dead-head Pressure Transducer, Knitted 2.0 mL, 240 V (Alprostadil)

1155-1096 Onyx, Dual Pump, Custom Reactor Volume, 120 V

1155-1097 Onyx, Dual Pump, Custom Reactor Volume, 240 V

1155-1098 Onyx, Single Pump, Custom Reactor Volume, 240 V

1155-1099 Onyx, Single Pump, Custom Reactor Volume, 120 V

10
Pickering Laboratories Product Catalog

Instruments & Hardware

Vector PCX

Vector PCX Derivatization Matched with Pickering Reagent Pumps

Instrument Laboratories' Chromatographic • Independently adjustable,
Grade® reagents, eluants low-pulsation
The Vector PCX serves as another
and columns, the Vector PCX
post-column choice ideal for • Adjustable from 0.05 to 2.00 mL/
instrument is a solid choice
application-specific methods. minute against back-pressures of
for laboratories’ standard post-
Vector PCX provides the selectivity up to 2000 psi
column needs.
and sensitivity required for most • Flow Accuracy 3 %
standard post-column applications Specifications • Flow Precision 0.5 % RSD
while being reliable and easy to
Dimensions • Sapphire pistons
use. Since the Vector PCX does not
have a column oven it is important • (h x w x d): 43 x 21.6 x 41.2 cm • Liquid ends, including check
to use the HPLC column oven to (17 x 8.75 x 16 inches) valve housing, PEEK®
ensure stable column temperature • PEEK® bypass/purge valves for
Weight
and prevent retention time drifts each pump located on front of
and separation problems. • 27.6 lbs (12.5 kg) –Duplex instrument panel
Electrical • Automatic piston wash
Features
• 100 - 120 V, 50/60 Hz
• Inert pumps Flow Path
1.7 A, 200 W or 200-240 V,
• PEEK liquid manifolds
®
50/60 Hz, 0.8 A, 200 W • Independent pressure transducer
for each pump 210 bar (0-3000 psi)
• Integrated gas manifold • Mains voltage ± 10 % of nominal
• Flow restrictors, matched to flow
• Easy access and monitoring • Installation (over voltage) rate and viscosity of reagents
of fluidics category II, pollution degree 2
• Simple control interface • PEEK® bypass/purge valves
• Indoor use only
(no computer required) • Replaceable reagent filter
Environmental
• Reduced bench space profile • PEEK® mixing manifold
• Altitude up to 6,500 ft
• Ambient temperature 15 - 25 ºC
• Relative humidity up to 80 %
at 31 ºC

11
 Pickering Laboratories Product Catalog

Instruments & Hardware

Vector PCX
Continued

Reactor • Manifold has two 1/4-28 tubing reagent pumps and reactor when
• Heater reactor controls at ± 0.4 ˚C connections the eluant pump pressure drops
for temperatures from 5 ˚C above • Gas line with anti-siphon valve to 30 bar (425 psi), ensuring that
ambient to 130 ˚C reagent will not flow upstream
Pressurized Reagent Reservoir and damage the analytical
• Range of reactor dwell volumes,
• One liter capacity (2 L reservoirs column. Low eluant pressure can
0.1 mL to 3.5 mL
available) result from power failure, eluant
• Reaction coil withstands up to pump malfunction, automatic
42 bar (600 psi) inlet pressure • Maintained under inert gas or intentional shut-down, or an
at 130 ˚C pressure to inhibit oxidation of empty reservoir. The Vector PCX
oxygen-sensitive reagents will not restart automatically.
• LCD display of actual temperature
or set point • Valve built into reservoir cap • Post-column system over
permits sparging during reagent pressure: A pre-calibrated relief
• Thermal safety switch limits
preparation valve opens at 35 bar (500 psi)
temperature to 150 ˚C to prevent
damage • Reagent reservoirs fitted with to prevent rupture of the post-
3.1 mm (1/8’’) OD, oxygen-imper- column reactor tubing in the
Gas Pressure Manifold and Regulator meable Air Barrier tubing for event of down-stream blockage
• Panel mounted manifold oxygen-sensitive reagents and/or • Back-pressure regulator: Applies
with 3.1 mm (1/8’’) OD FEP tubing 7 bar (100 psi) to the detector
• Regulator maintains 0.3 bar
(3-5 psi) on reagent reservoirs with Safeguards flow cell outlet (waste) to prevent
3-5 bar (45-75 psi) source pressure detector noise and precipitation
• A pressure switch installed
due to out-gassing or boiling
• Safety pressure relief valve opens between LC (eluant) pump and
at 0.7 bar (10 psi) sample injector turns off power to

Vector PCX Derivatization Instruments

Catalog No. Description
Vector, Single Pump, PEEK® Flow Restrictor, 0.15 mL, 120 V (Primary Amino Acids w/OPA,
1154-4011
Aminoglycoside Antibiotics, Biogenic Amines, Sulfonamide Artificial Sweetener)
Vector, Single Pump, PEEK® Flow Restrictor, 0.15 mL, 240 V (Primary Amino Acids w/OPA,
1154-4012
Aminoglycoside Antibiotics, Biogenic Amines, Sulfonamide Artificial Sweetener)
1154-4021 Vector, Single Pump Trione Flow Restrictor, 0.5 mL, 120 V (Amino Acids w/TRIONE®, Theanine)
1154-4022 Vector, Single Pump Trione Flow Restrictor, 0.5 mL, 240 V (Amino Acids w/TRIONE®, Theanine)
1154-4031 Vector, Single Pump, PEEK® Flow Restrictor, Knitted 1.4 mL, 120 V (Aflatoxins, Sulfonamide Drugs)
1154-4032 Vector, Single Pump, PEEK® Flow Restrictor, Knitted 1.4 mL, 240 V (Aflatoxins, Sulfonamide Drugs)
1154-4051 Vector, Dual Pump, PEEK® Flow Restrictors, 0.5 mL, 120 V (Carbamate, Glyphosate, Primary and Secondary Amino Acids w/OPA, Streptomycin)
1154-4052 Vector, Dual Pump, PEEK® Flow Restrictors, 0.5 mL, 240 V (Carbamate, Glyphosate, Primary and Secondary Amino Acids w/OPA, Streptomycin)
1154-4061 Vector, Dual Pump, PEEK® Flow Restrictors, Knitted 1.0 mL, 120 V (Paralytic Shellfish Toxins)
1154-4062 Vector, Dual Pump, PEEK® Flow Restrictors, Knitted 1.0 mL, 240 V (Paralytic Shellfish Toxins)
1154-4071 Vector, Dual Pump, PEEK® Flow Restrictors, Knitted 1.2 & 1.6 mL, 120 V (Tricothescene)
1154-4072 Vector, Dual Pump, PEEK® Flow Restrictors, Knitted 1.2 & 1.6 mL, 240 V (Tricothescene)
1154-4081 Vector, Dual Pump, Reverse Plumbing, PEEK® Tubing Flow Restrictors, Knitted 1.4 mL, 120 V (Polyether Antibiotics)
1154-4082 Vector, Dual Pump, Reverse Plumbing, PEEK® Tubing Flow Restrictors, Knitted 1.4 mL, 240 V (Polyether Antibiotics)
1154-4091 Vector, Single Pump, Custom Configuration, 120 V *
1154-4092 Vector, Dual Pump, Custom Configuration, 120 V *
1154-4093 Vector, Single Pump, Custom Configuration, 240 V *
1154-4094 Vector, Dual Pump, Custom Configuration, 240 V *
1154-4101 Vector, Single Pump, PEEK® Flow Restrictor, Knitted 3.5 mL, 120 V (Voglibose)
1154-4102 Vector, Single Pump, PEEK® Flow Restrictor, Knitted 3.5 mL, 240 V (Voglibose)
1154-4103 Vector, Single Pump, PEEK® Flow Restrictor, 0.5 mL, 120 V (Formaldehyde, B Vitamins)
1154-4104 Vector, Single Pump, PEEK® Flow Restrictor, 0.5 mL, 240 V (Formaldehyde, B Vitamins)
* Quote required for custom configurations. Contact support@pickeringlabs.com for questions.

12
Pickering Laboratories Product Catalog

Instruments & Hardware

UVE™

UVE™
Photochemical Reactor
Used for detection enhancement for Aflatoxins, Phenylurea Pesticides, Barbiturates and other compounds.
Photochemical derivatization is a simple, inexpensive and flexible technique that improves sensitivity and
selectivity of detection for a broad range of analytes. Among the applications for the photochemical reactor
are analysis of Aflatoxins in foods, Phenylurea Pesticides in water and Barbiturates in biological samples.
Photochemical derivatization also allows for the identification of closely related compounds such as polyphenols.
Pickering Laboratories Multi-residue Mycotoxins method for DON, Aflatoxins, Fumonisins, Ochratoxin A and
Zearalenone employs photochemical derivatization for Aflatoxins, allowing detection at sub-ppb levels.
The photochemical reactor has a 254 nm lamp and a knitted reactor coil.

Feature Highlights
• 254 nm UV low pressure lamp with cooled reflector tube
• Long term stability of lamp and coil
• High light transmission
• Robust steel housing to meet laboratory requirements
• Specially designed fluorocarbon coil
• Comparable to electrochemical derivatization with Cobra Cell
• AOAC accepted methodology
• Standard reactor volume is 1.0 mL

13
 Pickering Laboratories Product Catalog

Instruments & Hardware

UVE™
Continued

UV Derivatization Results in
Clear Peaks for Aflatoxins
Note the short run time: B1 elutes at 9.5 Min UVE ™

HPLC
10,000

FLD
9000

8000
B2 Easy Handling: Simply place the UVE™ between
7000 G2 your HPLC device and detector, switch it on -
ready to use!

6000
Intensity

5000

4000

3000 What actually happens? Aflatoxins B1 and G1 are

transformed to stable fluorescent derivatives resulting
2000 in larger peaks

1000
G1 B1 Sampling

0 Homogenization

0 1 2 3 4 5 6 7 8 9 10 Extraction
Min
Clean-up of Extract
Without UVE™: Low response for G1 and B1
Chromotography
10,000
Derivatization
B1
9000 Detection

Interpretation
8000
G1

7000
UVE™ Specifications

6000 CE Certified Yes

B2 UV Lamp 254 nm
Intensity

5000 Reactor Coil Special

G2
Dimensions 14.5 x 8.5 x 27 cm
4000 Power Input 50 W
Weight 3 kg
3000

2000 UVE™ Catalog Information

Catalog No. Description

1000 UVE™ -  Photochemical  Reactor for Aflatoxin

10519
Analysis, 240 V
UVE™ -  Photochemical  Reactor for Aflatoxin
0 10742
Analysis, 120 V
Reactor Coil (only for UVE™ reactor), 1 mL;
0 1 2 3 4 5 6 7 8 9 10 10520
other coil sizes on request 
Min
10563 UV replacement lamp 
With UVE™: High response and no band spreading

14
Pickering Laboratories Product Catalog

Chromatograms, Columns & Guards

Pickering Laboratories columns and guards are About Guard Columns

intended for specific applications that require post- While it is true that any of our columns may be
column derivatization. This technology guarantees run without a guard, the practical consequence is
detection of certain classes of compounds at very low a shorter column lifetime. Pickering Laboratories
concentrations—amino acids, carbamate pesticides sells a variety of appropriate guard columns to
and polyamines, for example. protect our analytical columns. Additionally, GARD™
Each column is packed and tested to separate provides protection against contamination for cation-
the target compounds according to Pickering’s exchange applications without affecting column
chromatographic quality control standards and efficiency and it is far less expensive to replace than an
published analytical method. The following analytical column. See page 17 for more information
acceptance criteria apply to all of Pickering’s about our GARD™ column protection system.
columns:
• With guard column installed, produce a specified
chromatogram of the compounds in a standard
test mixture.
• Separate the compounds in the established order,
with specified resolution of critical pairs.
• Operate within the specified range of
back pressure.
• Be free of contaminating material which can cause
baseline artifacts.
• Only after all criteria have been met can the
column’s serial number and label be applied.
• Quite simply, the column is guaranteed to produce
a chromatogram for its intended application if
it is operated according to the conditions and
methods prescribed by Pickering Laboratories.

15
 Pickering Laboratories Product Catalog

Chromatograms, Columns & Guards

Cation-Exchange Columns & Guards Reversed-Phase Columns & Guards

Lithium Columns Carbamate Columns

Catalog No. Description Catalog No. Description

70-minute High-efficiency Lithium Cation-exchange Carbamate Column, high resolution/capacity, C8 , 4.6 x 250 mm,
0846250
0354675T Column, 4.6 x 75 mm,  includes Amino Acid Test Mixture includes Carbamate Test Mixture 1700-0063
1700-0070
Carbamate Column, expanded resolution, C8, 4.0 x 250 mm,
0840250
High-efficiency Lithium Cation-exchange Column, includes Carbamate Test Mixture 1700-0063
0354100T
4.0 x 100 mm, includes Amino Acid Test Mixture 1700-0070
Carbamate Column, rapid analysis, C18, 4.6 x 150 mm,
1846150
Standard Lithium Cation-exchange Column, 3.0 x 250 mm, includes Carbamate Test Mixture 1700-0063
0393250
includes Amino Acid Test Mixture 1700-0070
18ECG001 Guard Cartridge Holder with 3 guard cartridges

18ECG002 Guard Cartridges, 2/pk.

Sodium Columns

Catalog No. Description

Polyether Antibiotics Column
30-minute High-efficiency Sodium Cation-exchange
1154110T Column 4.6 x 110 mm, includes Amino Acid Test Mixture Catalog No. Description
1700-0070
2381750 Polyether Reversed-phase Column, 4.6 x 250 mm
High-efficiency Sodium Cation-exchange Column for pro-
1154150T tein, collagen and oxidized feed hydrolysates, 4.0 x 150 mm, 18ECG001 Guard Cartridge Holder with 3 cartridges
includes Amino Acid Test Mixture 1700-0070
18ECG002 Guard Cartridges, 2/pk.
High-efficiency Sodium Cation-exchange Column for pro-
1154150 tein and collagen hydrolysates, 4.0 x 150 mm, includes
Amino Acid Test Mixture 1700-0070
Mycotox Column
Standard Sodium Cation-exchange Column 3.0 x 250 mm,
1193250
includes Amino Acid Test Mixture 1700-0070 Catalog No. Description

1612124 MYCOTOX Reversed-phase Column, 4.6 x 250 mm

™

Glyphosate Column 18ECG001 Guard Cartridge Holder with 3 cartridges

Catalog No. Description 18ECG002 Guard Cartridges, 2/pk.

Cation-exchange Column for Glyphosate analysis, 4 x 150 mm,

1954150
including  Glyphosate Test Mixture 1700-0080

GARD™ Column Protection System

( For use with any cation-exchange column )
Catalog No. Description
Cation-exchange GARD™ Assembly: Includes holder and 2
1700-3102
replaceable GARDs™
1700-3101 Replacement Cation-exchange GARDs™ (2/PK)

1700-3100 GARD™ Holder

Alkion Column

Catalog No. Description

9410917 ALKION™ Cation-exchange Column, K+ form, 4.0 x 150 mm

9493020 ALKION™ Guard Column, K+ form, 3.0 x 20 mm

16
Pickering Laboratories Product Catalog

Chromatograms, Columns & Guards

Cation-Exchange GARD™ Column

Protection System
Cation-exchange GARD™ uses a proprietary material to prevent matrix
compounds from passing through (and thereby protecting the analytical
column), but allows the analytes of interest to pass unimpeded through
the GARD™ and onto the analytical column.
The GARD™ significantly prolongs column life without band spreading
or added pressure. We demonstrated, by means of a performance
comparison for Amino Acid Analysis, that the use of a GARD™ will
effectively protect the analytical column, will be more cost-effective
for the laboratory, is easy to change, is universal to cation-exchange
applications, and most importantly has zero band spreading.
With GARD™ Column protection system from Pickering Laboratories the
same cation-exchange GARD™ can be used for nearly all cation-exchange
applications.

GARD™ Column Protection System ( For use with any cation-exchange column )

Catalog No. Description

1700-3102 Cation-exchange GARD™ Assembly: Includes holder and 2 replaceable GARDs™

1700-3101 Replacement Cation-exchange GARDs™ (2/PK)

1700-3100 GARD™ Holder

Lithium Amino Acid Analysis Columns

Pickering Laboratories specialize in manufacturing of cation-exchange columns for amino acid analysis. No
other techniques, including reversed-phase chromatography, have been shown to match post-column ion-ex-
change methods in quantitation and reproducibility. Advantages of this method, such as absence of matrix
interferences, are especially important in the analysis of native samples.
Lithium columns and buffers systems have high selectivity and are perfect for physiological fluids
and food analysis.
Post-column Conditions for Amino Acids Analysis: The recommended gradient conditions are subject to
Reagent: Trione® change without notice. This may happen because of
lot-specific changes in the columns, or improvements
Reactor: 130 °C, 0.5 mL
in the overall method.
Reagent Flow Rate: 0.3 mL/min
The recommended gradient for the column will
Detection: always be included in the column package and it
supersedes the information in this catalog. Use the
UV-Vis Detector: λ
 =570 nm for primary amino acids
program recommended on the column data sheet
λ=440 nm for secondary amino acids
for the initial testing.
or
Column oven temperature programming gives
Reagent: 300 mg of OPA, 2 g Thiofluor™, 3 mL of 30 % additional flexibility when optimizing methods. Using
Brij® 35 solution in 950 mL of OD104 a temperature gradient allows the user to improve
Reactor: 45 °C, 0.15 mL separation, shorten analysis time and fine-tune the
method for detecting compounds of interest.
Reagent Flow Rate: 0.3 mL/min

Detection:
Fluorometer: λex 330 nm, λem 465 nm

17
 Pickering Laboratories Product Catalog

Chromatograms, Columns & Guards

70-Min High-Efficiency Lithium Cation-Exchange Column (4.6 X 75 mm)

Catalog Number 0354675T
Use With Cation-Exchange Gard™ Column Protection System 1700-3102
Use for Physiological Samples
Temperature Gradient

HPLC Program Column Oven Program For more information see

Method Abstract MA 382
Time 1700-1125 % Li365 % Li375 % RG003 % Time Temp ºC and Pickering Amino Acids
0 100 0 0 0 0 34 Brochure.
10 100 0 0 0 6 34
19 40 60 0 0 17 65
32 0 100 0 0 25 70
43 0 100 0 0 70 70
43.1 0 0 100 0 71 34
57 0 0 100 0
57.1 0 0 70 30
72 0 0 70 30
72.1 100 0 0 0
HPLC Flow Rate: 0.55 mL/min, Initial Temp.: 34 ºC
Injection Volume: 10 µl of 0.25 µmole/ml std.
(P/N 1700-0170 with added Glutamine, AEC,
Glucosaminic acid, and Allo-Isoleucine.)

20
23

15
17 45
16
18 21
36
19 24
2 8
5 7 25 37
11 30 39
22 26 27 38
44 35
10
40

9 34
3 13 32 43
31
28 29 33 41 42
:

14
4 6 12

0 10 20 30 40 50 60 70 min

1 Phosphoserine 13 a-Aminoadipic acid 25 Leucine 37 Lysine

2 Taurine 14 Proline 26 Tyrosine 38 1-Methylhistidine
3 Phosphoethanolamine 15 Glycine 27 Phenylalanine 39 Histidine
4 Urea 16 Alanine 28 b-Alanine 40 3-Methylhistidine
5 Aspartic acid 17 Citrulline 29 b-Amino-i-butyric acid 41 Anserine
6 Hydroxyproline 18 a-Amino-n-butyric acid 30 Homocystine 42 Carnosine
7 Threonine 19 Valine 31 g-Aminobutyric acid 43 Arginine
8 Serine 20 Cystine 32 Tryptophan 44 Glucosaminic Acid*
9 Asparagine 21 Methionine 33 Ethanolamine 45 2-Aminoethyl-cysteine (AEC)*
10 Glutamic acid 22 Allo-Isoleucine 34 Ammonia
11 Glutamine 23 Cystathionine 35 Hydroxylysines
12 Sarcosine 24 Isoleucine 36 Ornitine

*Internal Standard

NOTE: This method utilizes column temperature gradient.

Use Onyx PCX column oven or Pinnacle PCX column oven
with temperature gradient capabilities.

18
Pickering Laboratories Product Catalog

Chromatograms, Columns & Guards

High-Efficiency Lithium Cation-Exchange Column (4.0 X 100 mm)

Catalog Number 0354100T
Use With Cation-Exchange Gard™ Column Protection System 1700-3102
Use for Physiological Samples and Complex Matrices
Temperature Isocratic

12

44
3 17 22
16 20
15 18 39
5 40
78
19
30
10 21 23
9 24 25 31 41
37 45
38
26 27 29 42
13 28 34 43
1112 33 35
32
4 14 36
6

0 25 75 50 100

1 Phosphoserine 13 a–Aminoadipic acid 25 Norleucine 37 Ornithine

2 Taurine 14 Proline 26 Tyrosine 38 Lysine
3 Phosphoethanolamine 15 Glycine 27 Phenylalanine 39 Histidine
4 Urea 16 Alanine 28 b–Alanine 40 3-Methyl-histidine
5 Aspartic acid 17 Citrulline 29 b–Amino-i-butyric acid 41 1-Methyl-histidine
6 Hydroxyproline 18 a–Amino-n-butyric acid 30 Homocystine 42 Carnosine
7 Threonine 19 Valine 31 g–Aminobutyric acid 43 Anserine
8 Serine 20 Cystine 32 Tryptophan 44 a–Amino–b–
9 Asparagine 21 Methionine 33 Ethanolamine guanidinopropionic acid
10 Glutamic acid 22 Cystathionine 34 Hydroxylysines 45 Arginine
11 Glutamine 23 Isoleucine 35 Ammonia
12 Sarcosine 24 Leucine 36 Creatinine

Conditions
Step Time (Min) Interval Li275 % Li750 % RG003 %
0 0 0 100 0 0
1 8 8 100 0 0
2 46 38 65 35 0
3 86 40 0 100 0
4 90 4 0 100 0
5 115 25 0 94 6
6 122 2 0 94 6
7 122.1 0.1 100 0 0
8 140 17.9 100 0 0
Flow Rate: 0.35 mL/min, Column Temp: 37 ºC,
Injection Volume: 10 µl of 0.25 µmole/ml std.
(P/N 011006P with added Glutamine)

For more information see Pickering Amino Acids Brochure.

19
 Pickering Laboratories Product Catalog

Chromatograms, Columns & Guards

High-Efficiency Lithium Cation-Exchange Column (4.0 X 100 mm)

Catalog Number 0354100T
Use With Cation-Exchange Gard™ Column Protection System 1700-3102
Use for Physiological Fluids
Temperature Gradient

Conditions Column Oven Program

Step Time (Min) 1700-1125 % Li365 % Li375 % RG003 % Time (Min) Temp ºC
0 0 100 0 0 0 0 34
1 15 100 0 0 0 13 34
2 27 40 60 0 0 30 65
3 45 0 100 0 0 67 66
4 60 0 100 0 0 80 70
5 60.1 0 0 100 0 97 70
6 78 0 0 100 0 98 34
7 78.1 0 0 70 30
8 95 0 0 70 30
9 95.1 100 0 0 0
10 115 100 0 0 0
HPLC Flow Rate: 0.4 mL/min, Initial Temp.: 34 ºC
Injection Volume: 10 µL of 0.25 µmole/mL Std.
(P/N 1700-0170 with added Glutamine and Glucosaminic acid)

20 22

16
2
15 17
18

19 21 35
8
5 7 36
23 24
29 37 38
3 43 34
10 39
25 26
9 32
13 31 33 42

30 41
11 40
For more information
27 28
12 14 see Pickering Amino
4 6 Acids Brochure.

0 20 40 60 80 min

1 Phosphoserine 12 Sarcosine 23 Isoleucine 34 Hydroxylysines

2 Taurine 13 a-Aminoadipic acid 24 Leucine 35 Ornitine
3 Phosphoethanolamine 14 Proline 25 Tyrosine 36 Lysine
4 Urea 15 Glycine 26 Phenylalanine 37 1-Methylhistidine
5 Aspartic acid 16 Alanine 27 b-Alanine 38 Histidine
6 Hydroxyproline 17 Citrulline 28 b-Amino-i-butyric acid 39 3-Methylhistidine
7 Threonine 18 a-Amino-n-butyric acid 29 Homocystine 40 Anserine
8 Serine 19 Valine 30 g-Aminobutyric acid 41 Carnosine
9 Asparagine 20 Cystine 31 Tryptophan 42 Arginine
10 Glutamic acid 21 Methionine 32 Ethanolamine 43 Glucosaminic Acid*
11 Glutamine 22 Cystathionine 33 Ammonia
*Internal Standard

NOTE: This method utilizes column temperature gradient. Use Onyx PCX column oven or
Pinnacle PCX column oven with temperature gradient capabilities.

20
Pickering Laboratories Product Catalog

Chromatograms, Columns & Guards

Standard Lithium Cation-Exchange Column 0393250 (3.0 X 250 mm)

Catalog Number 0393250
Use With Cation-Exchange Gard™ Column Protection System 1700-3102
Use for Physiological Fluids, Complex Matrices
Temperature Isocratic

1
2

3
44

22
40
20 39

5
8 1718 30 41
7 16 19

23 31
10 15 24
25 45
42
21 37 38
9 26 27 29 34
28 35 43
13
12 33
11 32
4 6 14 36

0 20 40 60 80 100 120 140 160 180

min
1 Phosphoserine 24 Leucine
2 Taurine 25 Norleucine
3 Phosphoethanolamine 26 Tyrosine Conditions
4 Urea 27 Phenylalanine Time (Min) Li275 % Li750 % RG003 %
5 Aspartic acid 28 b-Alanine
0 100 0 0
6 Hydroxyproline 29 b-Amino-i-butyric acid
17 100 0 0
7 Threonine 30 Homocystine
8 Serine 31 g-Aminobutyric acid 65 65 35 0
9 Asparagine 32 Tryptophan 128 0 100 0
10 Glutamic acid 33 Ethanolamine 145 0 100 0
11 Glutamine 34 Hydroxylysines
185 0 94 6
12 Sarcosine 35 Ammonia
185.1 100 0 0
13 a-Aminoadipic acid 36 Creatinine
14 Proline 37 Ornithine 210 100 0 0
15 Glycine 38 Lysine Flow Rate: 0.3 mL/min, Column Temp.: 40 ºC,
16 Alanine 39 Histidine Injection Volume: 10 µL of 0.25 µmole/mL Std.
(P/N 011006P with added Glutamine)
17 Citrulline 40 3-Methyl-histidine
18 a-Amino-n-butyric acid 41 1-Methyl-histidine
19 Valine 42 Carnosine
20 Cystine 43 Anserine
21 Methionine 44 a-Amino-b-
22 Cystathionine guanidino propionic acid
23 Isoleucine 45 Arginine

21
 Pickering Laboratories Product Catalog

Chromatograms, Columns & Guards

High-Efficiency Lithium Cation-Exchange Column (4.0 x 100 mm)

Catalog Number 0354100T
Use With Cation-Exchange Gard™ Column Protection System 1700-3102
Use for PKU/MSUD Screening of Physiological Fluids
Temperature Isocratic
a-Amino-n-Butyric Acid

Cystathionine
Valine

Methionine

Isoleucine

Norleucine

Phenylalanine
Leucine

Tyrosine

5 10 15 20 25 min

Conditions
Step Time (Min) Interval Li275 % Li750 % RG003 %
0 0 0 86 14 0
1 25 11 73 27 0
3 25.1 0.1 0 0 100
4 30 16.9 0 0 100
7 30.1 0.1 86 14 0
8 42 14.9 86 14 0
HPLC Flow Rate: 0.35 mL/Min, Column Temp.: 38 ºC
Injection Volume: 10 µl of 0.25 µmole/mL Std.

Lithium Columns

Catalog No. Description

0354675T 70-minute High-efficiency Lithium Cation-exchange Column, 4.6 x 75 mm,  includes Amino Acid Test Mixture 1700-0070

0354100T High-efficiency Lithium Cation-exchange Column, 4.0 x 100 mm, includes Amino Acid Test Mixture 1700-0070

0393250 Standard Lithium Cation-exchange Column, 3.0 x 250 mm, includes Amino Acid Test Mixture 1700-0070

GARD™ Column Protection System ( For use with any cation-exchange column )

Catalog No. Description

1700-3102 Cation-exchange GARD™ Assembly: Includes holder and 2 replaceable GARDs™

1700-3101 Replacement Cation-exchange GARDs™ (2/PK)

1700-3100 GARD™ Holder

22
Pickering Laboratories Product Catalog

Chromatograms, Columns & Guards

Sodium Amino Acid

Analysis Columns
Ion-exchange chromatography followed by post-
column derivatization has been a method of choice
for amino acid analysis for many years. Pickering
Laboratories' ion-exchange columns allow you to
obtain consistent results with sensitivity, stability,
selectivity and speed.
Sodium columns and buffers systems are designed
for amino acids analysis of hydrolyzed samples.

Post-column Conditions For Amino Acids Analysis:

Reagent: Trione®
Reactor: 130 °C, 0.5 mL
Reagent Flow Rate: 0.3 mL/min

Detection:
UV-Vis Detector: λ=570 nm for primary amino acids
λ=440 nm for secondary amino acids
or
Reagent: 300 mg of OPA, 2 g Thiofluor™, 3 mL of 30 %
Brij® 35 solution in 950 mL of OD104
Reactor: 45 °C, 0.15 mL
Reagent Flow Rate: 0.3 mL/min

Detection:
Fluorometer: λex 330 nm, λem 465 nm

The recommended gradient conditions are subject

to change without notice. This may happen because
of lot-specific changes in the columns, or improve-
ments in the overall method.
The recommended gradient for the column will
always be included in the column package, and it
supersedes the information in this catalog. Use the
program recommended on the column data sheet
for the initial testing.
The column oven temperature programming gives
additional flexibility when optimizing methods. Using
a temperature gradient allows the user to improve
separation, shorten analysis time and fine-tune the
method for detecting compounds of interest.

23
 Pickering Laboratories Product Catalog

Chromatograms, Columns & Guards

30-min High-Efficiency Sodium Cation-Exchange Column (4.6 x 110 mm)

Catalog Number 1154110T
Use With Cation-Exchange Gard™ Column Protection System 1700-3102
Use for Protein and Oxidized Feeds Hydrolysate
Temperature Gradient

16
Method for Protein Hydrolysate Samples Column
Oven Program
TIME Na315 % Na425 % Na640 % RG011 %
6 8 15 17 Time Temp ºC
0 100 0 0 0
10 0 46
7 12 4.0 100 0 0 0
23 9 14
11 13 4 46
1 20 15.0 0 100 0 0
4
9 70
18 19 16.0 0 0 100 0
32 70
31.0 0 0 100 0
5 33 46
31.1 0 0 0 100

0 5 10 15 20 25 30 33.0 0 0 0 100
33.1 100 0 0 0
Fig 1. Chromatogram of protein hydrolysate standard
40 100 0 0 0
HPLC Flow Rate: 0.6 mL/Min
Initial Temp.: 46 ºC
1 Aspartic Acid 9 Valine 17 Lysine
Injection Volume: 10 µL of 0.25 µmole/mL Std.
2 Threonine 10 Methionine 18 Tryptophan (P/N 012506H with added Norleucine)
3 Serine 11 Isoleucine 19 Ammonia
4 Glutamic Acid 12 Leucine 20 Arginine
5 Proline 13 Norleucine 21 Cysteic Acid
For more information see Method Abstract 380.1
6 Glycine 14 Tyrosine 22 Methionine
(page 76) and Pickering Amino Acids Brochure.
7 Alanine 15 Phenylalanine Sulfone
8 Cystine 16 Histidine NOTE: This method utilizes column temperature gradient.
Use Onyx PCX column oven or Pinnacle PCX column oven
with temperature gradient capabilities.

16
Method for Oxidized Feeds Column
6 8 Hydrolysate Samples Oven Program
21 14
TIME Na270 % Na425 % Na640 % RG011 % Time Temp ºC
15 0 100 0 0 0
17 0 55
7 10 13
4
9 12 4.0 100 0 0 0 12 55
22 11
15.0 0 100 0 0 17 70
123 16.0 0 0 100 0 32 70
20
31.0 0 0 100 0 33 55
18
31.1 0 0 0 100
5
33.0 0 0 0 100
33.1 100 0 0 0
0 5 10 15 20 25 30 min 40 100 0 0 0
Fig 2. Chromatogram of oxidized feeds hydrolysate standard HPLC Flow Rate: 0.6 mL/min
Initial Temp.: 55 ºC
Injection Volume: 10 µL OF 0.25 µmole/mL Std.
1 Aspartic Acid 9 Valine 17 Lysine
2 Threonine 10 Methionine 18 Tryptophan
3 Serine 11 Isoleucine 19 Ammonia For more information see Method Abstract 380.1
4 Glutamic Acid 12 Leucine 20 Arginine and Pickering Amino Acids Brochure.
5 Proline 13 Norleucine 21 Cysteic Acid
NOTE: This method utilizes a column temperature gradient.
6 Glycine 14 Tyrosine 22 Methionine Use Onyx PCX column oven or Pinnacle PCX column oven
7 Alanine 15 Phenylalanine Sulfone with temperature gradient capabilities.
8 Cystine 16 Histidine

24
Pickering Laboratories Product Catalog

Chromatograms, Columns & Guards

High-Efficiency Cation-Exchange Column (4.0 X 150 mm)

Catalog Number 1154150T
Use With Gard™ Column Protection System 1700-3102
Use for Oxidized Hydrolysate, Protein Hydrolysate, Collagen Hydrolysate
Temperature Isocratic
8
9 Conditions for Oxidized Samples 1154150T
20
11 Time (Min) Na270 % Na740 % RG011 %
23 18
13 Oxidized 0 100 0 0
14
Hydrolysate 14 100 0 0
32 20 80 0
24 4 5 16 32.1 0 100 0
3
6 19 56 0 100 0
22
56.1 0 0 100
58 0 0 100
7
58.1 100 0 0

0 10 20 30 40 50 min 70 100 0 0
Flow Rate: 0.4 mL/Min, Column Temp.: 50 ºC,
3 Aspartic Acid 14 Leucine Injection Volume: 10 µL of 0.25 µmole/mL Std.
4 Threonine 16 Phenylalanine (P/N 1700-0155)
5 Serine 18 Lysine
6 Glutamic Acid 19 Ammonia
7 Proline 20 Histidine
8 Glycine 22 Arginine
9 Alanine 23 Cysteic Acid
11 Valine 24 Methionine Sulfone
13 Isoleucine

10 1112 18 20
9
13
14
8
5
11 Protein 34
Collagen
9 12 18 20 10
Hydrolysate Hydrolysate
1314
8 17
5 1 1516
34 6
15 19
16
6
22
19
22 7
2
21
7

0 10 20 30 40 50 min 0 10 20 30 40 50 min

1 Methionine-D,L,-Sulfoxide 13 Isoleucine
2 trans-4-Hydroxy-L-Proline 14 Leucine Conditions for Protein & Collagen Hydrolysate Samples
3 Aspartic Acid 15 Tyrosine
Time (Min) Na315 % Na740 % RG011 %
4 Threonine 16 Phenylalanine
5 Serine 17 D,L & allo-Hydroxylysine 0 100 0 0

6 Glutamic Acid 18 Lysine 10 100 0 0

7 Proline 19 Ammonia 30 0 100 0
8 Glycine 20 Histidine 53 0 100 0
9 Alanine 21 Tryptophan
53.1 0 0 100
10 Cystine 22 Arginine
11 Valine 55 0 0 100

12 Methionine 55.1 100 0 0

67 100 0 0
Flow Rate: 0.4 mL/Min, Column Temp.: 48 ºC,
Injection Volume: 10 µL of 0.25 µmole/mL Std.
(P/N 012506H and 012506C)

25
 Pickering Laboratories Product Catalog

Chromatograms, Columns & Guards

Expanded Amino Acid Analysis of Hydrolyzed Samples

Catalog Number 1154150T

Use With Cation-Exchange Gard™ Column Protection System 1700-3102
Use for Oxidized and Non-oxidized Samples
Temperature Gradient

22 Conditions for Oxidized

1 21
and Non-Oxidized Samples
Time (Min) Na270 % Na740 % RG011 %
20
0 100 0 0
2 3 16 100 0 0
6 13 141516
10 11 40 54 46 0
5
4 1718 45 0 100 0
9 12
7 66 0 100 0
24
66.1 0 0 100
19
70 0 0 100
23
70.1 100 0 0
8
80 100 0 0
Flow Rate: 0.4 mL/min, Initial Temp.: 55 ºC,
0 10 20 30 40 50 60 min Injection Volume: 10 uL of 0.25 µmole/mL Std.
(P/N 1700-0165 with added Ornithine)

1 Cysteic Acid 7 Glutamic Acid 13 Methionine 19 Ammonia

2 Taurine 8 Proline 14 Isoleucine 20 Ornithine Column Oven Program
3 Methionine Sulfone 9 Glycine 15 Leucine 21 Lysine
Time Temp ºC
4 Aspartic Acid 10 Alanine 16 Norleucine 22 Histidine
0 55
5 Threonine 11 Valine 17 Tyrosine 23 Tryptophan
6 Serine 12 Cystine 18 Phenylalanine 24 Arginine 32 55
33 65
41 65
42 55

Sodium Columns

Catalog No. Description

30-minute High-efficiency Sodium Cation-exchange Column 4.6 x 110 mm, includes

1154110T
Amino Acid Test Mixture 1700-0070

High-efficiency Sodium Cation-exchange Column for protein, collagen and oxidized feed
1154150T
hydrolysates, 4.0 x 150 mm, includes Amino Acid Test Mixture 1700-0070
High-efficiency Sodium Cation-exchange Column for protein and collagen hydrolysates,
1154150
4.0 x 150 mm, includes Amino Acid Test Mixture 1700-0070
Standard Sodium Cation-exchange Column 3.0 x 250 mm, includes Amino Acid Test
1193250
Mixture 1700-0070

GARD™ Column Protection System

( For use with any cation-exchange column )
Catalog No. Description

1700-3102 Cation-exchange GARD™ Assembly: Includes holder and 2 replaceable GARDs™

1700-3101 Replacement Cation-exchange GARDs™ (2/PK)

1700-3100 GARD™ Holder

26
 Pickering Laboratories Product Catalog

Chromatograms, Columns & Guards

Carbamate Pesticide Analysis Columns

Pickering Laboratories carbamate columns are guaranteed to produce the separation of carbamate residues,
specified by EPA 531.1, EPA 531.2 and AOAC 985.23 methods.

Post-column conditions for pesticide analysis: Detection:

Reagent 1: Hydrolysis reagent CB130 or CB130.2 Fluorometer: λex 330 nm, λem 465 nm
Reagent 2: 100 mg of OPA, 2 g Thiofluor ™
The recommended gradient conditions are subject
in 950 mL of CB910 to change without notice. This may happen because
Reactor 1: 100 °C, 0.5 mL of lot-specific changes in the columns, or improve-
ments in the overall method.
Reactor 2: Ambient, 0.1 mL
The recommended gradient for the column will
Reagents Flow Rate: 0.3 mL/min always be included in the column package, and it
supersedes the information in this catalog.

High Resolution / Capacity Carbamate Column 0846250 (4.6 x 250 mm), C8, 5 µm
4

750 1
6
2 25 ng of Carbamates in 10 µL
Response [mV]

700
3
5 9
650
7
8 10
600 11
12
550

500

10 15 20 25 30 35 40 45
Time [min]

1 Aldicarb sulfoxide 5 3-Hydroxycarbofuran 9 Carbaryl

2 Aldicarb sulfone 6 Aldicarb 10 1-Naphthol
3 Oxamyl 7 Propoxur 11 Methiocarb
4 Methomyl 8 Carbofuran 12 BDMC

Conditions for Aqueous Samples Conditions for Methanolic Samples

Time (Min) Water % MeOH % Time (Min) Water % MeOH %
0 100 0 0 85 15
1 100 0 1 85 15
1.1 82 18 44 25 75
36 30 70 44.1 0 100
39 30 70 49 0 100
39.1 0 100 49.1 85 15
41 0 100 57 85 15
41.1 100 0 Flow Rate: 1 mL/min,
55 100 0 Column Temp.: 42 ºC,
Injection Volume: 10 mL
Flow Rate: 1 mL/min,
Column Temp.: 42 ºC,
Injection Volume: Up to 400 mL

27
 Pickering Laboratories Product Catalog

Chromatograms, Columns & Guards

Rapid Analysis Carbamate Column 1846150 (4.6 x 150 mm), 5 µm

4 4 ng of Carbamates in 150 µL
Conditions for
1
Aqueous Samples 2
6 9
Time (Min) Water % MeOH % 3
0 100 0 5
7
1 100 0 8 10 11
12
1.1 88 12
26 30 70
26.1 0 100 0 5 10 15 20 25 30
28 0 100 min
28.1 100 0
38 100 0
Flow Rate: 1 mL/Min,
Column Temp.: 42 ºC,
Injection Volume: Up To 250 mL

25 ng of Carbamates in 10 µL
Conditions for 4
Methanolic Samples
9
Time (Min) Water % MeOH % 6
2
0 85 15 1
3 5 7
0.5 85 15 10
8 11
28.5 30 70 12

28.6 0 100
33.5 0 100
33.6 85 15 0 5 10 15 20 25 30
41 85 15 min
Flow Rate: 1 mL/min, 1 Aldicarb sulfoxide 7 Propoxur
Column Temp.: 42 ºC,
2 Aldicarb sulfone 8 Carbofuran
Injection volume: 10 mL
3 Oxamyl 9 Carbaryl
4 Methomyl 10 1-Naphthol
5 3-Hydroxycarbofuran 11 Methiocarb
6 Aldicarb 12 BDMC (internal standard)

28
Pickering Laboratories Product Catalog

Chromatograms, Columns & Guards

Expanded Resolution Carbamate Column 0840250 (4.0 x 250 mm), 5 µm

4 25 ng of Carbamates in 10 µL
Conditions for
Water/Methanol Gradient 9
Time (Min) Water % MeOH % 6
1 2
0 85 15
2 85 15 3
42 30 70 5 7 10

46 30 70 11
8
46.1 0 100 12

51 0 100
51.1 85 15
59 85 15
Flow Rate: 0.8 mL/min, 0 5 10 15 20 25 30 35 40 45 50
Column Temp.: 50 ºC, min
Injection Volume: 10 mL for
methanolic and up to 400 mL for
aqueous samples

Conditions for 40 ng of Carbamates in 400 µL

Water/Acetonitrile Gradient
9
Time (Min) Water % ACN %
0 90 10 3
1 4
6
2 90 10 11
7
46 49 51 5 12
46.1 30 70 8
49 30 70
49.1 90 10 10

59 90 10 2

Flow Rate: 0.8 mL/min,

Column Temp.: 50 ºC,
Injection Volume: 10 mL for 0 5 10 15 20 25 30 35 40 45 50
methanolic and up to 400 mL
min
for aqueous samples
1 Aldicarb sulfoxide 5 3-Hydroxycarbofuran 9 Carbaryl
2 Aldicarb sulfone 6 Aldicarb 10 1-Naphthol
3 Oxamyl 7 Propoxur 11 Methiocarb
4 Methomyl 8 Carbofuran 12 BDMC (internal standard)

29
 Pickering Laboratories Product Catalog

Chromatograms, Columns & Guards

Separation of 23 Carbamates Using 0840250 Column (4.0 x 250 mm)

4
Conditions for
Water/Methanol Gradient
9
Time (Min) Water % MeOH %
1 6
0 85 15 2
2 85 15
3
42 30 70 5 7 N
46 30 70
8 10
46.1 0 100 11
51 0 100
51.1 85 15
59 85 15
4
Flow Rate: 0.8 mL/min,
20
Column Temp.: 50 ºC,
Injection Volume: 10 mL for methanolic 12
and up to 400 mL for aqueous samples 13
9
6
2 16
1 Aldicarb sulfoxide
2 Aldicarb sulfone 7
3 Oxamyl 14 15
17
4 Methomyl 1
5 3-Hydroxy
6 Aldicarb
7 Propoxur
8 Carbofuran 19
9 Carbaryl
10 Methiocarb 21
11 BDMC internal standard 5
12 Butocarboxim sulfoxide
13 Butocarboxim sulfone
14 Ethiofencarb sulfoxide
15 Ethiofencarb sulfone 11
3 18
16 Butocarboxim
17 Ethiofencarb
22 8 10
18 Thiofanox sulfoxide
19 Thiofanox sulfone 23
20 Methiocarb sulfoxide
21 Methiocarb sulfone
22 3-Ketocarbofuran 5 10 15 20 25 30 35 40 45 min
23 Thiofanox
N 1-Naphthol

Carbamate Columns

Catalog No. Description

0846250 Carbamate Column, high resolution/capacity, C8 , 4.6 x 250 mm, includes Carbamate Test Mixture 1700-0063

0840250 Carbamate Column, expanded resolution, C8, 4.0 x 250 mm, includes Carbamate Test Mixture 1700-0063

1846150 Carbamate Column, rapid analysis, C18, 4.6 x 150 mm, includes Carbamate Test Mixture 1700-0063

18ECG001 Guard Cartridge Holder with 3 guard cartridges

18ECG002 Guard Cartridges, 2/pk.

30
Pickering Laboratories Product Catalog

Chromatograms, Columns & Guards

Glyphosate Herbicide
Analysis Column
Pickering Laboratories cation-exchange Glyphosate
column is designed and tested for analysis of
Glyphosate and its primary metabolite Aminomethyl-
phosphonic acid (AMPA). This short, isocratic method
provides separation of the peaks of interest. Repro-
ducible performance is guaranteed run-to-run and
column-to-column.

Post-column Conditions For Glyphosate Analysis:

Reagent 1: Oxidizing reagent—100 µL of 5 % Sodium
Hypochlorite in 950 mL of GA116
Reagent 2: 100 mg of OPA, 2 g Thiofluor™
in 950 mL of GA104
Reactor 1: 36 °C, 0.5 mL
Reactor 2: Ambient, 0.1 mL
Reagents Flow Rate: 0.3 mL/min

Detection:
Fluorometer: λex 330 nm, λ
 em 465 nm

Glyphosate Column 1954150 (4.0 x 150 mm) K+ Cation-Exchange.

To be Used With Cation-Exchange GARD™ Column Protection System 1700-3102
Glyphosate

Conditions
AMPA
TIME (Min) K200 % RG019 %
0 100 0
15 100 0
15.1 0 100
17 0 100
17.1 100 0
27 100 0
Flow Rate: 0.4 mL/min,
0 10 20 min Column Temp.: 55 ºC,
Injection Volume: 10 mL
Glyphosate Test Mixture, 2.5 µg/mL

For more information see Method Abstract

MA 206 and MA 207 (pages 93 and 95).

Glyphosate Column GARD™ Column Protection System

( For use with any cation-exchange column )
Catalog No. Description
Catalog No. Description
Cation-exchange Column for Glyphosate analysis, 4 x 150 mm,
1954150 Cation-exchange GARD™ Assembly: Includes holder and
including  Glyphosate Test Mixture 1700-0080 1700-3102
2 replaceable GARDs™
1700-3101 Replacement Cation-exchange GARDs™ (2/PK)

1700-3100 GARD™ Holder

31
 Pickering Laboratories Product Catalog

Chromatograms, Columns & Guards

Alkion™ Column

Histamine
The stationary phase in the ALKION™ column is a rigid,
non-porous polymeric phase that is surface sulfon-

Cadaverine
ated. The two modes of separation are ion-exchange

Putrescine

Spermidine
and moderate partitioning. The low capacity of the
ion-exchange resin makes it an ideal phase for the
separation of strongly basic and positively charged
compounds. The reversed-phase character allows for
0 5 10 15 20 min
discrimination between closely related species.
Chromatogram of fish sauce sample
The unique properties of this column allow for its use
in a wide range of applications.

Alkion™ Column Analysis of

Biogenic Amines Conditions

Post-column Conditions: Time (Min) K600 % K563 % K130 %

Reagent: 3
 00 mg of OPA, 2 g Thiofluor , 3 mL of ™ 0 100 0 0

30 % Brij® 35 in 950 mL of OD104 6 100 0 0

15 0 100 0
Reactor: 45 °C, 0.15 mL
21 0 100 0
Reagent Flow Rate: 0.3 mL/min 21.1 0 0 100

Detection: 23 0 0 100
23.1 100 0 0
Fluorometer: λex 330 nm, λem 465 nm
29 100 0 0
Flow Rate: 0.8 mL/min,
Column Temp.: 45 ºC,
Injection Volume: 10 mL
Histamine
Tyramine

Spermidine
Cadaverine
Putrescine

Spermine

0 5 10 15 20 min

Chromatogram of calibration standard, 100 µM

Histamine

Putrescine

Cadaverine

Spermidine

0 5 10 15 20 min

Chromatogram of fish sample

32
Pickering Laboratories Product Catalog

Chromatograms, Columns & Guards

Alkion™ Column Analysis of Aminoglycoside

Antibiotics in Feeds
Post-column Conditions:
Reagent: 3
 00 mg of OPA, 2 g Thiofluor™, 3 mL of
30 % Brij® 35 in 950 mL of OD104
Reactor: 45 °C, 0.15 mL
Reagent Flow Rate: 0.3 mL/min

Detection:
Fluorometer: λex 330 nm, λem 465 nm
Apramycin

Conditions for Apramycin Analysis

Time (Min) 1700-1101 % 1700-1102 % 1700-1103 %
0 67 33 0
5 67 33 0
15 14.7 7.3 78
20 14.7 7.3 78
20.1 0 22 78
21 0 22 78
21.1 67 33 0
28 67 33 0
0 5 10 15 20 25 30
Flow Rate: 0.8 mL/min, min
Column Temp.: 40 ºC,
Injection Volume: 10 mL
Gentamicin
Complex

Conditions for Gentamicin Analysis

Time (Min) 1700-1101 % 1700-1102 % 1700-1103 %
0 43 31 26
20 9 13 78
30 9 13 78
30.1 0 22 78
31 0 22 78
31.1 43 31 26
38 43 31 26
Flow Rate: 0.8 mL/min,
Column Temp.: 40 ºC,
Injection Volume: 10 mL 0 5 10 15 20 25 30
min

Conditions for Neomycin Analysis Neomycin

Time (Min) 1700-1101 % 1700-1102 % 1700-1103 %

0 60 40 0
15 13.2 8.8 78
25 11 11 78
25.1 0 22 78
26 0 22 78
26.1 60 40 0
32 60 40 0
Flow Rate: 0.8 mL/min,
Column Temp.: 40 ºC,
Injection Volume: 10 mL
0 5 10 15 20 25 30
min

33
 Pickering Laboratories Product Catalog

Chromatograms, Columns & Guards

Neomycin

Gentamicin Conditions for Separation of Apramycin

Complex Gentamicin and Neomycin
Time (Min) 1700-1101 % 1700-1102 % 1700-1103 %
Apramycin
0 60 40 0
15 13.2 8.8 78
15.1 12 10 78
30 12 10 78
30.1 0 22 78
31 0 22 78
31.1 60 40 0
37 60 40 0
Flow Rate: 0.8 mL/min,
Column Temp.: 40 ºC,
Injection Volume: 10 mL
0 5 10 15 20 25 30
min

34
 Pickering Laboratories Product Catalog

Chromatograms, Columns & Guards

Alkion™ Column Analysis of Streptomycin

Post-column Conditions: or
Reagent 1: 0.75 N Sodium Hydroxide Reagent 1: Oxidizing Reagent: 100 µL of 5 %
Reactor 1: ambient, 0.1 mL Sodium Hypochlorite in GA116
Reagent 2: 0.6 % Ninhydrin Reactor 1: 50 °C, 0.5 mL
Reactor 2: 60 °C, 0.5 mL Reagent 2: 300 mg of OPA, 2 g Thiofluor™, 3 mL of
30 % Brij® 35 solution in 950 mL of OD104
Reagents Flow Rate: 0.3 mL/min
Reactor 2: Ambient, 0.1 mL
Detection:
Detection:
Fluorometer: λex 395 nm, λem 500 nm
Fluorometer: λex 330 nm, λem 465 nm

Streptomycin

Conditions
Time (Min) 1700-1101 % 1700-1102 % 1700-1103 %
0 60 40 0
10 60 40 0
10.1 48 48 4
15 48 48 4
15.1 60 40 0
21 60 40 0
Flow Rate: 0.8 mL/min,
Column Temp.: 40 ºC,
0 5 10 15 Injection Volume: Up to several mL
min

Alkion Column

Catalog No. Description

9410917 ALKION™ Cation-exchange Column, K+ form, 4.0 x 150 mm

9493020 ALKION™ Guard Column, K+ form, 3.0 x 20 mm

35
 Pickering Laboratories Product Catalog

Chromatograms, Columns & Guards

Mycotox™ Reversed-phase B1

Column for Mycotoxins Analysis

Aflatoxins Analysis
Aflatoxins are naturally occurring toxins belong-
ing to the class Mycotoxins. They are produced by
fungi and occur in peanuts, peanut meal, cot- G1 B2
ton-seed meal, wheat, milk and many other foods
and feeds.
The most important feature of the post-column
method described below is that all six Aflatoxins
are detectable at the same fluorescence emission G2
wavelength in a single isocratic HPLC analysis.
With derivitization
M2
LC Conditions: M1
Flow Rate: 1.0 mL/min, column temperature 42 °C,
injection volume 10 µL
Mobile Phase: Methanol/acetonitrile/water:
(22:22:56), isocratic Without derivitization
Injection: 10 µL in Methanol
5 ng B1 & G1
1.5 ng B2 & G2
1.25 ng M1 & M2 0 2 4 6 8 10 12 14
min

Post-column Conditions:
Reagent: I2 100 mg/L in water For more information see Method Abstract
MA 215, MA 208, MA 203.1 and MA 218
Reactor: 90 °C, 1.4 mL
Reagent Flow Rate: 0.4 mL/min

Detection:
Fluorometer: λex 365 nm, λem 430 nm

Mycotox Column

Catalog No. Description

1612124 MYCOTOX Reversed-phase Column, 4.6 x 250 mm

™

18ECG001 Guard Cartridge Holder with 3 cartridges

18ECG002 Guard Cartridges, 2/pk.

36
Pickering Laboratories Product Catalog

Chromatograms, Columns & Guards

Polyether Antibiotics Analysis Column

Polyether antibiotics, such as Monensin, Narasin and Salinomycin, found in raw material, premix, liquid
supplements and final feeds, are best quantified using HPLC with post-column derivatization. Because
of the selectivity of the post-column reaction, almost no sample clean-up is needed.

LC Conditions: Post-column Conditions

Flow Rate: 0.7 mL/min, column temperature 40 °C, (non-metallic post-column derivatization system
injection volume 10 µL is required):
Mobile Phase:  0:10 of Methanol / 5 % Acetic
9 Reagent 1: Concentrated Sulfuric acid /
Acid in water, isocratic Methanol (4:96 v/v)
Reactor 1: Ambient, 0.1 mL
Reagent 2: 60 g of Vanillin (or p-dimethylaminobenz-
aldehyde) in 950 mL of Methanol
Reactor 2: 90 °C, 1.4 mL
2 Reagents Flow Rate: 0.3 mL/min
NOTE: Using 2-reagent system for this application
greatly extends life of reagents.
One-reagent method and post-column derivatization
Feed Extract
system are also available.

Detection:
1 UV-Vis Detector: Vanillin λ=520 nm, DMAB λ=450 nm

Standard
1

0 5 10 15
min

1 Monensin B 2 Monensin A 3 Narasin A

Polyether Antibiotics Column

Catalog No. Description

2381750 Polyether Reversed-phase Column, 4.6 x 250 mm

18ECG001 Guard Cartridge Holder with 3 cartridges

18ECG002 Guard Cartridges, 2/pk.

37
 Pickering Laboratories Product Catalog

Reagents, Diluents & Sample Preparation Solutions

Trione® Ninhydrin Reagent

Chromatographic Grade™
A Prepared Reagent for Post-column Derivatization
of Primary and Secondary Amines
TRIONE® Ninhydrin reagent is specially formulated for Amino Acid Analysis. It contains Ninhydrin, Hydrindan-
tin (reduced Ninhydrin), a Lithium Acetate buffer, and Sulfolane, a water-miscible organic solvent. The solvent
is necessary to maintain the solubility of both the Hydrindantin and the primary amine product, Ruhemann’s
Purple. The buffer is required because the reaction is pH dependent. The active ingredients - Ninhydrin and
Hydrindantin are required for proper development of secondary and primary amines, respectively.
TRIONE® is so stable that is does not require refrigeration, either in shipment, storage, or in the reservoir.
Quantitation is consistent from the first to the last mL, so there is no waste. The high signal-to-noise ratio of
TRIONE®, when compared to DMSO-containing reagents, permits detection sensitivity to be increased with
minimum increase in background noise - a feature particularly appreciated at sample concentrations
of <50 picomoles.

Two preparations are available to suit your usage and storage requirements:

T100 and T100C

• Pour into your reservoir and use; the ultimate in convenience with a minimum of handling.
• 4-month* shelf life

T200
• Combine the two provided solutions, swirl, and use.
• 12-month* shelf life before mixing; one month in the reservoir after preparation.

Trione® Ninhydrin Reagent Catalog Information

Catalog No. Description Quantity
T100 TRIONE® Ninhydrin Reagent (4-month* shelf life) Each ( 950 mL/bottle)
T100C TRIONE® Ninhydrin Reagent (4-month* shelf life) Case of 4 (950 mL/bottle)
T200 TRIONE® Two-part Ninhydrin Reagent (12-month* shelf life before mixing) To prepare case of 4 (900 mL/bottle)

* From date of manufacture

38
Pickering Laboratories Product Catalog

Reagents, Diluents & Sample Preparation Solutions

Thiofluor™
N,N-Dimethyl-2-Mercaptoethylamine Hydrochloride –
Chromatographic Grade™ for Post-column Derivatization
of Primary Amines
Primary amines form highly fluorescent adducts when reacted with
o-Phthalaldehyde (OPA) and a mercaptan under basic conditions.
The products of this reaction, 1-alkyl-2-thioalkyl-substituted isoindoles,
exhibit optimal excitation at 330 nm and maximal emission at 465 nm.
Pickering’s Thiofluor™, a solid, nearly odorless nucleophile, is a superior
substitute for 2-Mercaptoethanol in the preparation of OPA reagents. It
forms a more stable reagent and a longer-lasting fluorophore with OPA Thiofluor™
than does 2-Mercaptoethanol, yet it has the same fluorescence properties. Catalog No. Description
Unlike the volatile 2-Mercaptoethanol, Thiofluor™ will not migrate through
3700-2000 Thiofluor , each (10 g/bottle)
™

the gas manifold and regulator of the OPA reagent

pressurization system.
Two grams of Thiofluor™ is equivalent to 1 mL of 2-Mercaptoethanol.

o-Phthalaldehyde (OPA)
Chromatographic Grade™ for Post-column
Derivatization of Primary Amines
Primary amines form highly fluorescent adducts when reacted with
o-Phthalaldehyde (OPA) and a mercaptan under basic conditions.
The products of this reaction, 1-alkyl-2-thioalkyl-substituted isoindoles,
exhibit optimal excitation at 330 nm and maximal emission at 465 nm.
Pickering’s OPA is specially prepared to meet the demanding require-
ments of high-sensitivity pre- and post-column HPLC derivatization o-Phthalaldehyde (OPA)
methods. Our repurification process actually begins with commercially Catalog No. Description
available 99 % material.
o-Phthalaldehyde (OPA),
O120
The entire process is controlled to eliminate trace interfering contamina- each (5 g/bottle)
tion. Lot quality is verified by post-column HPLC using a high-sensitivity
fluorescence detector.

Vanillin
4-Hydroxy-3-Methoxybenzaldehyde Chromatographic Grade™
for Post-column Analysis of Polyether Antibiotics
Reagent formulation with Vanillin, Methanol and Sulfuric Acid allows
specific post-column analysis of polyether antibiotics, such as Monensin,
Narasin and Salinomycin. This reagent can also be used for the post-col-
umn derivatization of Sulfa Drugs. Polyether antibiotics are monitored at
520 nm and Sulfa Drugs at 400 nm.
Chromatographic Grade™ Vanillin meets the exacting purity require-
ments for a post-column chemical, ensuring that detection at 520 nm
will be free of reagent artifacts. Because it is sensitive to oxygen, Picker- Vanillin

ing Laboratories Vanillin is sealed under Argon gas. Catalog No. Description

3700-2200 Vanillin, each (30 g/bottle)

1700-2200 Vanillin, each (300 g/bottle)

39
 Pickering Laboratories Product Catalog

Reagents, Diluents & Sample Preparation Solutions

Naphthalene-2,3-dicarboxaldehyde
Chromatographic Grade™ for Derivatization of Primary
Amino Groups
Naphthalene-2,3-dicarboxaldehyde reacts with primary amines in
presence of a nucleophile (e.g., mercaptan) and forms highly fluorescent
1-Alkylbenz-2-thioalkyl[f]isoindole derivatives.
This reagent is especially effective for post-column or precolumn derivat-
ization of proteins, peptides and other large amines since the derivative
is stable and doesn’t internally quench. Naphthalene-2, 3-Dicarboxaldehyde

Naphthalene-2,3-dicarboxaldehyde can also be used as a reagent for Catalog No. Description

chemiluminescence analysis. 3700-0100
Naphthalene-2,3-dicarboxalde-
hyde, each (500 mg/bottle)

p-Dimethylaminobenzaldehyde (DMAB)
Chromatographic Grade™ for Post-column Analysis
of Sulfa Drugs in Feed and Animal Tissues
p-Dimethylaminobenzaldehyde (DMAB; Ehrlich’s Reagent) reacts rapidly
with many primary amines to form a complex with maximum absor-
bance at 450 nm.
Reversed-phase LC followed by post-column derivatization is used
to determine the levels of Sulfamethazine, Sulfathiazole and related
compounds in feeds, premixes and animal tissues. Although these
compounds have a UV chromophore, so do most of the other p-Dimethyl Amino Benzaldehyde (DMAB)
constituents in the complex sample matrix. Forming a colored Catalog No. Description
derivative post-column allows shifting of the detection wavelength p-Dimethylaminobenzaldehyde
3700-0400
into the visible range thus greatly enhancing selectivity. (DMAB), each (30 g/bottle)
p-Dimethylaminobenzaldehyde
1700-0400
(DMAB), each (300 g/bottle)

o-Phthalaldehyde (OPA) Diluent

Chromatographic Grade™ for Preparation of
o-Phthalaldehyde Reagents
The derivatization of primary amines with o-Phthalaldehyde and a
mercaptan requires basic conditions. To ensure the optimum pH of
the reaction, it is important to use the correct diluent.
Three application specific diluents are available : CB910 and GA104 for
Carbamate and Glyphosate analyses respectively, and OD104 for analysis
of amino acids and other amines.
These borate buffers are produced from starting materials which
are free of heavy metals and amines. As with most other products
manufactured by Pickering Laboratories, the quality of the OPA
Diluents is verified by actual post-column HPLC analysis. o-Phthalaldehyde (OPA) Diluent

Catalog No. Description

o-Phthalaldehyde (OPA) Diluent
CB910 for Carbamate Analysis, case of
4 (950 mL /bottle)
o-Phthalaldehyde (OPA) Diluent
GA104 for Glyphosate Analysis, case of
4 (950 mL /bottle)
o-Phthalaldehyde (OPA) Diluent
OD104 for Amino Acids and Amines
analysis, case of 4 (950/bottle)

40
Pickering Laboratories Product Catalog

Reagents, Diluents & Sample Preparation Solutions

Hydrolysis Reagent CB130

Chromatographic Grade™
This 0.05 M NaOH/C47™ reagent is applied in the first stage of post-column
Carbamate derivatization. At 100 ºC the separated carbamates are con-
verted from urethanes to methylamines. The methylamines can then
react with OPA and Thiofluor™ to form the characteristically fluorescent
isoindole.
Hydrolysis Reagent contains C47 ™ which prevents the precipitation
of insoluble minerals (e.g. Ca2+, Mg2+) from samples of hard drinking water
and ground water. The C47 ™ additive complexes these minerals and
keeps them in solution.

Hydrolysis Reagent CB130.2

Chromatographic Grade™ Hydrolysis Reagent
The new Hydrolysis Reagent CB130.2 is formulated according to USEPA Catalog No. Description
Method 531.2 and contains 0.075 M Sodium Hydroxide. This reagent is ap-
Hydrolysis Reagent for Carba-
plied during the first stage of post-column derivatization for the analysis CB130 mate analysis 0.05 M, case of
of Carbamate pesticides. 4 (950 mL /bottle)
Hydrolysis Reagent for Carba-
Hydrolysis Reagent CB130.2 contains C47 ™, which prevents the precipi- CB130.2 mate analysis 0.075 M, case of
tation of insoluble minerals from samples of hard drinking and ground 4 (950 mL /bottle)
water, and protects your instruments from damage.

Hypochlorite Diluent GA116

Chromatographic Grade™ for the Preparation of the Oxidizing
Reagent for Post-column Glyphosate Herbicide Analysis
This diluent is used to prepare the oxidizing reagent required to convert
Glyphosate to a primary amine prior to reacting it with OPA. The reagent
is prepared by adding 100 µL of 5 % solution of Sodium hypho­chlorite to
one 950 mL bottle of Diluent. The pH 11.6 diluent is formulated to ensure
pH stability of the mixed stream of column effluent and oxidizing reagent.
This diluent could also be used in fluorescence detection of other
secondary amines.

Hypochlorite Diluent

Catalog No. Description

Hypochlorite Diluent for
GA116 Glyphosate analysis,
case of 4 (950 mL/bottle)

41
 Pickering Laboratories Product Catalog

Reagents, Diluents & Sample Preparation Solutions

Li220 & Na220

Chromatographic Grade™ Samples and Standards
Diluents for Amino Acid Analysis
Use of these diluents is essential to ensuring reproducibility from injection
to injection. They establish a uniform pH and ion concentration at the
outset, regardless of the source and pre-treatment of the sample. The
sample is maintained buffered and at optimum pH for sample storage
and analysis.
Use Li220 for prepared physiological samples and to dilute amino acid
calibration standards for use with Lithium columns and buffers. Use
Na220 for hydrolysate samples and to dilute amino acid calibration stan-
dards for use with Sodium columns and buffers. Li220 & Na220

Catalog No. Description

SERAPREP & URIPREP ™ ™ Li220

Lithium Diluent, pH 2.36, case
of 4 (250 mL/bottle)
Sodium Diluent, pH 2.20, case
Chromatographic Grade™ Reagents for the Preparation Na220
of 4 (250 mL/bottle)
of Native Samples for Amino Acid Analysis
Preparation of samples such as serum, urine, other physiological fluids,
plant extracts, foods and beverages requires control of pH and normality,
as well as removal of soluble protein.
The samples must be held to a narrow pH range between 2.1 and 2.5, and
at the proper Lithium ion concentration to ensure reproducibility in the
early part of the chromatogram.
SERAPREP™ and URIPREP™ replace commonly used protein precipitation
reagents such as Acetonitrile, Perchloric acid, and Picric acid, and eliminate
the need for dialysis, ultrafiltration, and the repeated centrifugation steps,
followed by pH adjustment.
SERAPREP™ is used for preparing serum and other samples with high
buffering capacity, e.g. sardine oil. URIPREP™ is used for urine and other
samples with low buffering capacity, such as fruit juices, beer and wines.
SERAPREP™ & URIPREP™
The efficiency of protein precipitation and need for post-centrifugation
pH adjustment of the sample determine which reagent is best for your Catalog No. Description
particular sample. SERAPREP™, each
SP100
(250 mL/bottle)
URIPREP™, each
UP100
(250 mL/bottle)

42
Pickering Laboratories Product Catalog

Reagents, Diluents & Sample Preparation Solutions

Chlorac™ Buffer
Chromatographic Grade™ for Preservation
of Aqueous Carbamate Samples
Trace analysis of Carbamate insecticide residues in drinking water is a
mandated requirement in the United States. Because several of the com-
mon Carbamates—Carbaryl, Oxamyl, Hydroxycarbofuran - are labile in
water due to oxidation or hydrolysis, the samples and standards must be
preserved in order to obtain valid results.
ChlorAC™ Buffer from Pickering Laboratories is a highly purified Chro-
matographic Grade™ preservative. It is prepared from Monochloracetic
acid and Potassium acetate to EPA specifications. ChlorAC™ is guaranteed
to be free of co-eluting interferences for the analytes in EPA 531.1. ChlorAC™ Buffer

Catalog No. Description

RESTORE ™
1700-0132
ChlorAC Buffer, each
(250 mL/bottle)
ChlorAC Buffer, case of
Chromatographic Grade™ for Removal of Metal Ion 1700-0025
4 (25 mL/bottle)
Contamination and Restoration of Proper Ion Balance
in Glyphosate Analytical Columns and Guards
Glyphosate herbicide analysis by post-column derivitization and HPLC
according to US EPA Method 547 employs a cation-exchange column.
Many polyvalent metal ions which may be present in the sample,
especially Iron, will accumulate in the guard or analytical column. As little
as 100 nmole of Ferric Iron, for example, will cause serious degradation of
column performance; larger amounts can actually cause the Glyphosate
peak to vanish completely.
Glyphosate RESTORE™ rapidly removes Iron and all other polyvalent
metals from the column and guard. In addition, it preserves the balance
of K+/H+ in the resin, thus avoiding a lengthy re-equilibration.

RESTORE™

Catalog No. Description

1700-0140 RESTORE , each (250 mL/bottle)

™

43
 Pickering Laboratories Product Catalog

Eluants

Chromatographic Amino Acid Analysis, Sodium-Based Solutions

Grade™ Eluants Catalog No. Description

Na270 Sodium Eluant, pH 2.80, case of 4 (950 mL/bottle)

Pickering Laboratories eluants are manufactured
under strictly controlled conditions to guarantee Na315 Sodium Eluant, pH 3.15, case of 4 (950 mL/bottle)

purity, stability and consistency for a reproducible Na328 Sodium Eluant, pH 3.28, case of 4 (950 mL/bottle)
high quality chromatogram. This quality standard Na425 Sodium Eluant, pH 4.25, case of 4 (950 mL/bottle)
guarantees the resulting chromatogram will be free
Na640 Sodium Eluant, pH 6.40, case of 4 (950 mL/bottle)
of any noise and interference.
Na740 Sodium Eluant, pH 7.40, case of 4 (950 mL/bottle)
Lithium and Sodium eluants are not sensitive to
Sodium Eluant, pH 3.15, with 5 % Sulfolane,
oxidation and do not need refrigeration, either in 1700-0112
case of 4 (950 mL/bottle)
storage or use. Degassing or filtration is not required. Sodium Eluant, pH 3.33, with 2.5 % Sulfolane,
However, they should be protected from air to prevent 1700-0114
case of 4 (950 mL/bottle)
contamination. Ambient air actually contains amines RG011 Sodium Column Regenerant, each (950 mL/bottle)
and amino acids that will dissolve in the low-pH
Amino Acid Analysis, Lithium-Based Solutions
eluants and will appear in the chromatograms.
Li275 Lithium Eluant, pH 2.75, case of 4 (950 mL/bottle)
All buffers are packaged in cases of four 950 mL
Li280 Lithium Eluant, pH 2.80, case of 4 (950 mL/bottle)
polyethylene bottles. The regenerants are packaged
in single 950 mL bottles because of the small volumes Li292 Lithium Eluant, pH 2.92, case of 4 (950 mL/bottle)
used during each analysis. Sample diluents are 1700-1125 Lithium Eluant, pH 2.80, case of 4 (950 mL/bottle)
packaged in cases of four 250 mL borosilicate
Li365 Lithium Eluant, pH 3.65, case of 4 (950 mL/bottle)
glass bottles.
Li375 Lithium Eluant, pH 3.75, case of 4 (950 mL/bottle)

Custom Eluants
Li750 Lithium Eluant, pH 7.50, case of 4 (950 mL/bottle)

RG003 Lithium Column Regenerant, each (950 mL/bottle)

We develop and manufacture custom eluants for Glyphosate Herbicide Analysis
customers who require our “Guaranteed Chemistry”
Glyphosate Potassium Phosphate Eluant,
commitment of purity and certified use. Let us make K200
case of 4 (950 mL/bottle)
some for you! Call for details.
RG019 Glyphosate Column Regenerant, each (950 mL/bottle)
Potassium-Based Solutions for ALKION™ Column for Aminoglycoside
Antibiotics, Paraquat/Diquat in Water Analysis & Streptomycin
Potassium Phosphate Eluant, KO1,
1700-1101
case of 4 (950 mL/bottle)
Potassium Hydroxide Eluant, KO2,
1700-1102
case of 4 (950 mL/bottle)
Potassium Chloride Eluant, KO3,
1700-1103
case of 4 (950 mL/bottle)
Biogenic Amines/Polyamines Analysis
Potassium Column Regenerant,
K130
each (950 mL/bottle)
Potassium Phosphate Eluant, pH 5.63,
K563
case of 4 (950 mL/bottle)
Potassium Phosphate Eluant, pH 6.00,
K600
case of 4 (950 mL/bottle)
Multi-Residue Mycotoxin Analysis
Sodium Phosphate Eluant, pH 3.30,
1700-1108
case of 4 (950 mL/bottle)

44
Pickering Laboratories Product Catalog

Test Mixtures & Standards

Calibration Standards
• Quantitative and guaranteed
• Each lot tested chromatographically
• Chromatographically-pure starting components
Pickering’s Amino Acid Standards have a reputation
worldwide for quality and reliability in all post-column
systems and methods. Amino Acid Standard mixtures
are available for a variety of applications. The standards
are in 1 mL vials (5 per pack) in an appropriate citrate
buffer or 0.1 N HCl.
Although they are stored frozen at the factory, Picker-
ing’s calibration standards remain stable when shipped
at ambient temperatures. Upon receipt, however, it is
important to place them into a freezer immediately and
store frozen until ready for use.

Amino Acid Calibration Standards

Catalog No. Description

011006P Native Sample Standard with Norleucine in Lithium Citrate Buffer, 0.25 µmole/mL, pack (5x1 mL)

012006P Native Sample Standard without Norleucine in Lithium Citrate Buffer, 0.25 µmole/mL, pack (5x1 mL)

012506C Collagen Hydrolysate Standard in Sodium Citrate Buffer, 0.25 µmole/mL, Proline and Hydroxyproline 1.25 µmole/mL, pack (5x1 mL)

012506H Protein Hydrolysate Standard, in Sodium Citrate Buffer, 0.25 µmole/mL, pack (5x1 mL)

1700-0155 Oxidized Feed Hydrolysate Standard in Sodium Citrate Buffer, 0.25 µmole/mL, pack (5x1 mL)

1700-0165 Sodium Amino Acid Standard with Norleucine, 0.25 µmole/mL, pack (5x1 mL)
Native Sample Standard without Norleucine & Alpha-Amino-Beta-Guanidinopropionic Acid in Lithium Citrate Buffer, 0.25 µmole/mL,
1700-0170
pack (5x1 mL)
1700-0175 Native Sample Standard, Basics, in 0.1 N HCl, 2.5 µmole/mL, pack (5x1 mL)

1700-0180 Native Sample Standard, Acidics and Neutrals, in 0.1 N HCl, 2.5 µmole/mL, Cystine 1.25 µmole/mL, pack (5x1 mL)

Carbamate Calibration Standards

1700-0185 Carbamate Internal Standard pack (5x1 mL)

1700-0190 Carbamate Calibration Standard pack (5x1 mL)

Test Mixtures
• Qualitative mixtures
• For troubleshooting and establishing chromatographic analyte profile only

Test Mixtures

Catalog No. Description

1700-0070 Amino Acid Test Mixture in 0.01 N HCl, 0.25 µmole/mL, each (5.0 mL/bottle), L-Cysteic Acid, L-Threonine, L-Serine
Carbamate Test Mixture in Methanol, 2.5 µg/mL, each (5.0 mL /bottle), Aldicarb, Aldicarb Sulfone, Aldicarb Sulfoxide,
1700-0063 DMC (4-Bromo-3,5-dimethylphenyl-N-methylcarbamate), Carbaryl, Carbofuran, 3-Hydroxycarbofuran, Methomyl, Methiocarb,
1-Naphthol, Oxamyl, and Propoxur
1700-0080 Glyphosate Test Mixture in Water, 2.5 µg/mL, each (5.0 mL /bottle), Glyphosate, AMPA (Aminoethyl-phosphonic acid)

45
 Pickering Laboratories Product Catalog

Test Mixtures & Standards

Calibration Standards for Amino Acid Analysis

Constituents 1700-0180* 1700-0175* 1700-0170 011006P 012006P 1700-0165 012506C 012506H 1700-0155
Beta-Alanine • • • •
Alanine • • • • • • • •
D,L-a-Amino-adipic acid • • • •
Gamma-Amino butyric acid • • • •
Alpha-Amino-n-butyric acid • • • •
D,L,b-Amino-i-butyric acid • • • •
Alpha-Amino-Beta-
• •
guanidinopropionic acid
Ammonia • • • • • • • •
Anserine • • • •
Arginine • • • • • • • •
Asparagine • • • •
Aspartic acid • • • • • • • •
Carnosine • • • •
Citrulline • • • •
Creatinine • • • •
Cystathionine • • • •
Cystine • (1.25) • • • • • •
Cysteic acid • •
Ethanolamine • • • •
Glutamic acid • • • • • • • •
Glycine • • • • • • • •
Histidine • • • • • • • •
D,L-Homocystine • • • •
L,L & allo-Hydroxylysine • • • • •
4-trans-L-Hydroxyproline • • • • • (1.25)
Isoleucine • • • • • • • •
Leucine • • • • • • • •
Lysine • • • • • • • •
Methionine • • • • • • •
Methionine-D,L-sulfoxide •
Methionine-D,L-sulfone • •
1-Methyl-histidine • • • •
3-Methyl-histidine • • • •
Norleucine • •
Ornithine • • • •
Phenylalanine • • • • • • • •
Phosphoethanolamine • • • •
Phosphoserine • • • •
Proline • • • • • • (1.25) • •
Sarcosine • • • •
Serine • • • • • • • •
Taurine • • • • •
Threonine • • • • • • • •
Tryptophan • • • • • •
Tyrosine • • • • • • •
Urea • • • •
Valine • • • • • • • •
NOTE: Concentration of all the constituents in the Amino Acid standards is 0.25 µmole/mL unless otherwise specified.

*Concentration of all the constituents is 2.5 µmole/mL unless otherwise specified

46
Pickering Laboratories Product Catalog

Clean-Up Columns

AflaCLEAN™, OtaCLEAN™, Afla-OtaCLEAN™

& DONex™ Clean-Up Columns
Immunoaffinity & SPE Sample Clean-Up Columns
Aflatoxins and Ochratoxin A are produced by fungi, e.g. Aspergillus and
Penicillium species. Therefore both toxin types are found together in
many foods and animal feeds, e.g. cereals. Deoxynivalenol, also known
as Vomitoxin, is a metabolite of various molds of the genus Fusarium
(F. colmorum, F. graminearum) and can often be found together with
other mycotoxins.
Of significant assistance is the clean-up of extracts by a combined
immunoaffinity column (the Afla-OtaCLEAN column) for both Aflatoxins
and Ochratoxin A in one step. The subsequent analysis may then be
performed by HPLC with post-column derivatization or other techniques.

Simultaneous Clean-Up of Aflatoxins Sample Clean-Up Column

and Ochratoxin A for Aflatoxin Analysis
The combination sample clean-up column AflaCLEAN™ from LCTech was developed for the
Afla-OtaCLEAN™ from LCTech is very tolerant towards sample clean-up of foods, grains, feeds, etc. for
many matrices and allows for a comprehensive aflatoxin analysis using HPLC post-column deri-
clean-up of the aflatoxins B1, B2, G1 and G2 as well as vatization or other techniques. The AflaCLEAN™
Ochratoxin A. The high maximum capacity of 150 ng column is selective for B1, B2, G1 and G2. The
for Aflatoxin B1 and 200 ng for Ochratoxin A provides maximum loading capacity is 150 ng of Aflatoxin.
a wide measurement range. With a shelf-life of 1 year The shelf-life is 2 years (from date of manufacture)
(from date of manufacture) at room temperature, the at room temperature. Our cost-effective AflaCLEAN
storage and use of the columns is very convenient. Select columns have a shelf-life of 9 months at 4 ºC.

Example Recoveries of Aflatoxin and Ochratoxins Example Recoveries of Aflatoxin

Matrix B1 B2 G1 G2 OTA Matrix B1 B2 G1 G2
Maize (Afla 10 ppb, Peanut Butter 95 % 98 % 93 % 84 %
107 % 91 % 103 % 75 % 97 %
OTA 14.3 ppb)
Peanuts 104 % 94 % 96 % 85 %
Rice (Afla 10 ppb,
107 % 93 % 98 % 85 % 101 % Dried Distillers
OTA 14.3 ppb) 109 % 97 % 90 % 77 %
Grain
Malt (Afla 10 ppb,
98 % 99 % 97 % 70 % 96 % Maize 101 % 98 % 103 % 80 %
OTA 14.3 ppb)
Raisins (Afla 10 ppb,
99 % 106 % 101 % 69 % 97 %
OTA 14.3 ppb)
Aflatoxin Sample Clean-Up Column

Catalog No. Description

Aflatoxins & Ochratoxin a Sample Clean-Up Column
AflaCLEAN™, Aflatoxins B1, B2, G1, G2, Pkg 25, 24 month*
10514
Afla-OtaCLEAN™, Aflatoxins B1, B2, G1, G2 & Ochratoxin A, shelf life at room temp.
11022 Pkg 25, 18 month* shelf life at room temp. (Minimum order AflaCLEAN™ SELECT, Aflatoxins B1, B2, G1, G2, Pkg 25, 9
of 20 packs) 12058
month* shelf life at 4 °C (Minimum order of 20 packs)
Afla-OtaCLEAN™, Aflatoxins B1, B2, G1, G2 & Ochratoxin A AflaCLEAN™ SELECT, Aflatoxins B1, B2, G1, G2, Pkg 25, 9
11771 , Pkg 500, 18 month* shelf life at room temp (Minimum 12062
month* shelf life at 4 °C (Minimum order of 20 packs)
order of 4 packs)
* From date of manufacture

47
 Pickering Laboratories Product Catalog

Clean-Up Columns

AflaCLEAN Select Columns

Catalog No. Description

AflaCLEAN SELECT, Aflatoxins B1, B2, G1, G2, Pkg 25,
™
12058
9 month* shelf life at 4 °C (Minimum order of 20 packs)
AflaCLEAN™ SELECT, Aflatoxins B1, B2, G1, G2, Pkg 500,
12059
9 month* shelf life at 4 °C (Minimum order of 4 packs)
AflaCLEAN™ SELECT, Aflatoxins B1, B2, G1, G2, Pkg 25,
12062
9 month* shelf life at 4 °C (Minimum order of 20 packs)
AflaCLEAN™ SELECT, Aflatoxins B1, B2, G1, G2, Pkg 500,
12063
9 month* shelf life at 4 °C (Minimum order of 4 packs)
* From date of manufacture

AflaCLEAN™ SMART Cartridges, Special Small Format

Catalog No. Description

AflaCLEAN™ SMART, Aflatoxins B1, B2, G1, G2, Pkg 100,
12862
9 month* shelf life at 4 °C (Minimum order of 8 packs)
AflaCLEAN™ SMART, Aflatoxins B1, B2, G1, G2, Pkg 1000,
12863
9 month* shelf life at 4 °C (Minimum order of 2 packs)

Emission: 460 nm
160 400 mV
mV
B1

125 300

100 G1

B2 200
75
G2
50
100

25

-20 -50
0 1.3 2.5 3.8 5.0 6.3 7.5 8.8 10.0 0.0 2.5 5.0 7.5 10.0
min min

Distillers grain spiked with 5 ppb Peanut Butter spiked with 5 ppb
total Aflatoxins total Aflatoxins

Emission: 440 nm
200 mV

Ota A

150

100

50

-20
0 1.3 2.5 3.8 5.0 6.3 7.5 8.8 10.0
min

Distillers grain spiked with

5 ppb Ochratoxin A

48
Pickering Laboratories Product Catalog

Clean-Up Columns

Sample Clean-Up Column

for Ochratoxin Analysis
OtaCLEAN™ was developed for sample clean-up of foods, grains, feeds, etc. for Ochra-
toxin A analysis using HPLC or other techniques. The antibody employed possesses a
very high Ochratoxin A specificity. This leads to exceptional chromatographic results
without any interfering secondary signals and very high recovery rates. Independent
of the complexity of the matrix, excellent results can be achieved.
The maximum loading capacity is 200 ng Ochratoxin A. The shelf-life is 2 years (from
date of manufacture) at room temperature.

700 mV
Example Recoveries of Ochratoxin A
Ota A
Matrix Recovery Rate
500
Dried Distillers Grain 102 %
Duran Wheat 92 %

250 Coffee 100 %

Red Wine 108 %
Rye Bran 91 %
0
Beer 96 %

0.0 2.5 5.0 7.5 10.0

Grapes 91 %
min
Horse Feed 94 %
Roasted Coffee spiked with Wort 107 %
5 ppb Ochratoxin A

OtaCLEAN™ Sample Clean-Up Columns

Catalog No. Description

10515 OtaCLEAN , Ochratoxin A, Pkg 25, 24 month* shelf life at room temp. (Minimum order of 20 packs)
™

Afla-OtaCLEAN™, Aflatoxins B1, B2, G1, G2 & Ochratoxin A, Pkg 25, 18 month* shelf life at room temp.
11022
(Minimum order of 20 packs)
11535 OtaCLEAN™, Ochratoxin A, Pkg 500, 24 month* shelf life at room temp. (Minimum order of 4 packs)

12425 OtaCLEAN™, Ochratoxin A, Pkg 25, 24 month* shelf life at room temp. (Minimum order of 20 packs)

12427 OtaCLEAN™, Ochratoxin A, Pkg 500, 24 month* shelf life at room temp. (Minimum order of 4 packs)

OtaCLEAN™ SMART Sample Clean-Up Columns

Catalog No. Description

OtaCLEAN™ SMART, Ochratoxin A, special small format, Pkg 100, 9 month shelf life* at 4 °C
13346
(Minimum order of 8 packs)
OtaCLEAN™ SMART, Ochratoxin A, special small format, Pkg 1000, 9 month shelf life* at 4 °C
13351
(Minimum order of 2 packs)
* From date of manufacture

49
 Pickering Laboratories Product Catalog

Clean-Up Columns

DONeX
Deoxynivalenol, also known as Vomitoxin, is a metabolite
of various molds of the genus Fusarium (F. culmorum,
F. graminearum) and can often be found on contami-
nated cereals (wheat, barley, oat).
Generally the toxin is analyzed with HPLC/UV detection
or alternatively with HPLC / post column derivatization /
fluorescence detection or LC / MS. Whichever method
is chosen, a good sample preparation extends life time
of the analyzer and of the HPLC column and reduces
interferences by matrix components. Moreover the
running times of the HPLC system can be reduced
for easy matrices from about 25 to 10 minutes by
pre-cleaning.
If HPLC/UV detection is used, sensitivity can be dra-
matically enhanced with larger sample volumes.
The DONeX column has a matrix load of up to 4 grams.
It is ideally suited to many different matrices, including
corn, barley, wheat, animal feeds, muesli, and bread.

Chromatogram of a cereal-based poultry feed sample,

DONeX ™, SPE Clean-Up Cartridge for DON Analysis, 3 mL
analyzed with HPLC/post column derivatization/FLD,
Catalog No. Description sample was spiked with 1 ppm DON, extracted and
DONeX™, SPE Clean-up Cartridge for DON Analysis, Pkg 25, purified, evaporated sample (1 g) was resolved in 2 mL
12792
no expiration at room temp. (Minimum order of 20 packs) HPLC solvent, 40 μL were injected (0.02 gram matrix
DONeX™, SPE Clean-up Cartridge for DON Analysis, equivalents represent 20 ng DON injected)
12793 Pkg 500, no expiration at room temp. (Minimum order
of 4 packs)

DONeX Column Recoveries

Matrix Recoveries [%] DON
Bread 108
Corn 90
Chicken Feed 101
Distillers Grain 91
Oat Flakes 100
Pasta (Dried) 105
Poultry Feed 100
Rice 104
Rye 91
Wheat 100
Wheat Bran 99
* All matrices were spiked prior to the extraction. Spiking solution
was incubated for at least 1 h with matrix before extraction was
started. Calculation of recovery is based on subtraction of values
obtained without spiking.

Recoveries for Nivalenol are similar to those observed

for Deoxynivalenol.

50
Pickering Laboratories Product Catalog

Clean-Up Columns

Sample Preparation
Extraction:
Take 25 g of homogenous sample and add enough
water (after estimation of moisture content) to make
the total volume of water 125 mL. Blend and Centrifuge.
Matrix Specific Modification:
• High water content – reduce sample amount to 12.5 g
• High protein content – add 100 µL HCl to 20 mL of
extract, shake and centrifuge.
• High fat content – perform the methylene chloride
partition twice.

Clean-Up

 lyphosate and AMPA Sample

G Methylene Chloride Partition:
• To 20 mL of aqueous extract add 15 mL methylene
Clean-Up Cartridges chloride. Shake for 2-3 min and centrifuge.
Pickering SPE sample clean-up cartridges are • To 4.5 mL of aqueous layer add 0.5 mL acidic
used to treat samples prior to injection. The strong modifier solution. Shake and centrifuge.
cation-exchange resin is composed of sulfonic
acid functional groups attached to a styrene SPE Clean-Up:
divinylbenzene copolymer lattice. The high selectivity • Prepare SPE cartridge (refer to product
and ruggedness provides a simple and universal abstract PA210)
method for removing matrix interference from the • Transfer 1 mL portions of SPE mobile phase (refer
sample. The SPE sample clean-up cartridges are to method abstract MA206 for formulation) and
ideal for the clean-up of vegetables, fruits and crop discard the effluent.
samples in the analysis of Glyphosate and AMPA.
• Elute analytes with 12 mL SPE mobile phase
Recoveries for Glyphosate and AMPA Concentration:
Matrix Glyphosate AMPA • Evaporate to dryness using rotary evaporator or
Alfalfa 129 % 116 % a vacuum vortex-type evaporator or lyophilize
Strawberry 84 % 82 %
• Re-dissolve in 2 mL of the SPE mobile phase
Broccoli 97 % 95 %

Glyphosate Sample Clean-Up Columns

Catalog No. Description

1705-0001 Glyphosate SPE Sample Clean-up Columns, 50/pkg

51
 Pickering Laboratories Product Catalog

Application Kits

Amino Acid Analysis, 1700-3102

Cation-exchange GARD™ assembly: Holder
w/ 2 replaceable GARDs™
Sodium-Based Solutions Na220 Sodium Diluent, pH 2.20, case of 4 (250 mL/bottle)

Amino Acid Analysis, Sodium-Based Solutions Na270 Sodium Eluant, pH 2.80, case of 4 (950 mL/bottle)

Catalog No. Description Na425 Sodium Eluant, pH 4.25, case of 4 (950 mL/bottle)

0352-0061 30-Minute High Efficiency Collagen Hydrolysate Kit: Na640 Sodium Eluant, pH 6.40, case of 4 (950 mL/bottle)

30-minute High-efficiency Sodium Cation-exchange RG011 Sodium Column Regenerant, each (950 mL/bottle)
1154110T Column 4.6 x 110 mm, includes Amino Acid Test Mixture
TRIONE® Ninhydrin Reagent (4-month* shelf life),
1700-0070 T100C
case of 4 (950 mL/bottle)
Cation-exchange GARD™ assembly: Holder
1700-3102 Oxidized Feed Hydrolysate Standard in Sodium Citrate Buf-
w/ 2 replaceable GARDs™ 1700-0155
fer, 0.25 µmole/mL, each (5 mL/bottle)
Na220 Sodium Diluent, pH 2.20, case of 4 (250 mL/bottle)
0352-0021 Kit Identical to O352-0020 with T200 Replacing T100C:
Na315 Sodium Eluant, pH 3.15, case of 4 (950 mL/bottle) TRIONE® Two-part Ninhydrin Reagent (12-month* shelf life
T200
before mixing), to prepare case of 4 (900 mL/bottle)
Na425 Sodium Eluant, pH 4.25, case of 4 (950 mL/bottle)
0352-0022 Kit Identical to O352-0020 with OPA Replacing T100C:
Na640 Sodium Eluant, pH 6.40, case of 4 (950 mL/bottle)
OD104 OPA Diluent, case of 4 (950 mL/bottle)
RG011 Sodium Column Regenerant, each (950 mL/bottle)
O120 o-Phthalaldehyde, each (5 g/bottle)
TRIONE Ninhydrin Reagent (4-month* shelf life),
®
T100C
case of 4 (950 mL/bottle) 3700-2000 Thiofluor™, each (10 g/bottle), (2 per kit)
Collagen Hydrolysate Standard in Sodium Citrate Buffer, AT30SH Standard 60-Minute Protein Hydrolysate Kit:
012506C 0.25 µmole/mL, Proline and Hydroxyproline 1.25 µmole/mL,
each (5 mL/bottle) Standard Sodium Cation-exchange Column 3.0 x 250 mm,
1193250
includes Amino Acid Test Mixture 1700-0070
0352-0062 Kit Identical to O352-0061 with T200 Replacing T100C:
Cation-exchange GARD™ assembly: Holder
TRIONE® Two-part Ninhydrin Reagent (12-month* shelf life 1700-3102
T200 w/ 2 replaceable GARDs™
before mixing), to prepare case of 4 (900 mL/bottle)
Na328 Sodium Eluant, pH 3.28, case of 4 (950 mL/bottle)
0352-0063 Kit Identical to O352-0061 with OPA Replacing T100C:
Na740 Sodium Eluant, pH 7.40, case of 4 (950 mL/bottle)
OD104 OPA Diluent, case of 4 (950 mL/bottle)
TRIONE® Ninhydrin Reagent (4-month* shelf life),
O120 o-Phthalaldehyde, each (5 g/bottle) T100C
case of 4 (950 mL/bottle)
0352-0057 30-Minute High Efficiency Protein Hydrolysate Kit: RG011 Sodium Column Regenerant, each (950 mL/bottle)
30-minute High-efficiency Sodium Cation-exchange Na220 Sodium Diluent, pH 2.20, case of 4 (250 mL/bottle)
1154110T Column 4.6 x 110 mm, includes Amino Acid Test Mixture
1700-0070 Protein Hydrolysate Standard, in Sodium Citrate Buffer,
012506H
0.25 µmole/mL, each (5 mL/bottle)
Cation-exchange GARD assembly: Holder
™
1700-3102
w/ 2 replaceable GARDs™ 0352-0030 Kit Identical to AT30SH with T200 Replacing T100C:

Na220 Sodium Diluent, pH 2.20, case of 4 (250 mL/bottle) TRIONE® Two-part Ninhydrin Reagent (12-month* shelf life
T200
before mixing), to prepare case of 4 (900 mL/bottle)
Na315 Sodium Eluant, pH 3.15, case of 4 (950 mL/bottle)
AO30SH Kit Identical to AT30SH with OPA Replacing T100C:
Na425 Sodium Eluant, pH 4.25, case of 4 (950 mL/bottle)
OD104 OPA Diluent, case of 4 (950 mL/bottle)
Na640 Sodium Eluant, pH 6.40, case of 4 (950 mL/bottle)
O120 o-Phthalaldehyde, each (5 g/bottle)
RG011 Sodium Column Regenerant, each (950 mL/bottle)
3700-2000 Thiofluor™, each (10 g/bottle), (2 per kit)
TRIONE® Ninhydrin Reagent (4-month* shelf life),
T100C
case of 4 (950 mL/bottle) AT31FH High-Efficiency 55-Minute Protein Hydrolysate Kit:
Protein Hydrolysate Standard, in Sodium Citrate Buffer, High-efficiency Sodium Cation-exchange Column for
012506H
0.25 µmole/mL, each (5 mL/bottle) 1154150T protein, collagen and oxidized feed hydrolysates, 4.0 x 150
mm, includes Amino Acid Test Mixture 1700-0070
0352-0058 Kit Identical to O352-0057 with T200 Replacing T100C:
Cation-exchange GARD™ assembly: Holder
TRIONE® Two-part Ninhydrin Reagent (12-month* shelf life 1700-3102
T200 w/ 2 replaceable GARDs™
before mixing), to prepare case of 4 (900 mL/bottle)
Na315 Sodium Eluant, pH 3.15, case of 4 (950 mL/bottle)
0352-0059 Kit Identical to O352-0057 with OPA Replacing T100C:
Na740 Sodium Eluant, pH 7.40, case of 4 (950 mL/bottle)
OD104 OPA Diluent, case of 4 (950 mL/bottle)
TRIONE® Ninhydrin Reagent (4-month* shelf life),
O120 o-Phthalaldehyde, each (5 g/bottle) T100C
case of 4 (950 mL/bottle)
3700-2000 Thiofluor™, each (10 g/bottle), (2 per kit) RG011 Sodium Column Regenerant, each (950 mL/bottle)
30-Minute High Efficiency Oxidized Feed Na220 Sodium Diluent, pH 2.20, case of 4 (250 mL/bottle)
0352-0020
Hydrolysate Kit:
Protein Hydrolysate Standard, in Sodium Citrate Buffer,
30-minute High-efficiency Sodium Cation-exchange 012506H
0.25 µmole/mL, each (5 mL/bottle)
1154110T Column 4.6 x 110 mm, includes Amino Acid Test Mixture
1700-0070 0352-0031 Kit Identical to AT31FH with T200 Replacing T100C:

52
Pickering Laboratories Product Catalog

Application Kits

(Continued) Amino Acid Analysis, Sodium-Based Solutions

TRIONE® Two-part Ninhydrin Reagent (12-month* shelf life Cation-exchange GARD™ assembly: Holder
T200 1700-3102
before mixing), to prepare case of 4 (900 mL/bottle) w/ 2 replaceable GARDs™
AO31FH Kit Identical to AT31FH with OPA Replacing T100C: 1700-1125 Lithium Eluant, pH 2.80, case of 4 (950 mL/bottle)

OD104 OPA Diluent, case of 4 (950 mL/bottle) Li220 Lithium Diluent, pH 2.36, case of 4 (250 mL/bottle)

O120 o-Phthalaldehyde, each (5 g/bottle) Li365 Lithium Eluant, pH 3.65, case of 4 (950 mL/bottle)

3700-2000 Thiofluor™, each (10 g/bottle), (2 per kit) Li375 Lithium Eluant, pH 3.75, case of 4 (950 mL/bottle)

AT32FC High-Efficiency 55-Minute Collagen Hydrolysate Kit: RG003 Lithium Column Regenerant, each (950 mL/bottle)
High-efficiency Sodium Cation-exchange Column for Native Sample Standard without Norleucine &
1154150T protein, collagen and oxidized feed hydrolysates, 4.0 x 150 1700-0170 Alpha-Amino-Beta-Guanidinopropionic Acid in Lithium
mm, includes Amino Acid Test Mixture 1700-0070 Citrate Buffer, 0.25 µmole/mL, each (5 mL /bottle)
Cation-exchange GARD™ assembly: Holder SP100 SERAPREP™, each (250 mL/bottle)
1700-3102
w/ 2 replaceable GARDs™
UP100 URIPREP™, each (250 mL/bottle)
Na315 Sodium Eluant, pH 3.15, case of 4 (950 mL/bottle)
TRIONE® Ninhydrin Reagent (4-month* shelf life),
Na740 Sodium Eluant, pH 7.40, case of 4 (950 mL/bottle) T100C
case of 4 (950 mL/bottle)
TRIONE® Ninhydrin Reagent (4-month* shelf life), 0352-0007 Kit Identical to O352-0006 with T200 Replacing T100C:
T100C
case of 4 (950 mL/bottle)
TRIONE® Two-part Ninhydrin Reagent (12-month* shelf life
RG011 Sodium Column Regenerant, each (950 mL/bottle) T200
before mixing), to prepare case of 4 (900 mL/bottle)
Na220 Sodium Diluent, pH 2.20, case of 4 (250 mL/bottle) 0352-0008 Kit Identical to O352-0006 with OPA Replacing T100C:
Collagen Hydrolysate Standard in Sodium Citrate Buffer, OD104 OPA Diluent, case of 4 (950 mL/bottle)
012506C 0.25 µmole/mL, Proline and Hydroxyproline 1.25 µmole/mL,
each (5 mL/bottle) O120 o-Phthalaldehyde, each (5 g/bottle)

0352-0032 Kit Identical to AT32FC with T200 Replacing T100C: 3700-2000 Thiofluor™, each (10 g/bottle), (2 per kit)
TRIONE® Two-part Ninhydrin Reagent (12-month* shelf life Standard 185-Minute Physiologic Fluid/Native
T200 AT33SP
before mixing), to prepare case of 4 (900 mL/bottle) Sample Kit:
AO32FC Kit Identical to AT32FC with OPA Replacing T100C: Standard Lithium Cation-exchange Column, 3.0 x 250 mm,
0393250
includes Amino Acid Test Mixture 1700-0070
OD104 OPA Diluent, case of 4 (950 mL/bottle)
Cation-exchange GARD™ assembly: Holder
1700-3102
O120 o-Phthalaldehyde, each (5 g/bottle) w/ 2 replaceable GARD™s

3700-2000 Thiofluor™, each (10 g/bottle), (2 per kit) Li275 Lithium Eluant, pH 2.75, case of 4 (950 mL/bottle)

0352-0018 60-Minute Oxidized Feed Hydrolysate Kit: Li750 Lithium Eluant, pH 7.50, case of 4 (950 mL/bottle)

High-efficiency Sodium Cation-exchange Column TRIONE® Ninhydrin Reagent (4-month* shelf life),
T100C
1154150T for protein, collagen and oxidized feed hydrolysates, case of 4 (950 mL/bottle)
4.0 x 150 mm, includes Amino Acid Test Mixture 1700-0070
RG003 Lithium Column Regenerant, each (950 mL/bottle)
Cation-exchange GARD™ assembly: Holder
1700-3102 Li220 Lithium Diluent, pH 2.36, case of 4 (250 mL/bottle)
w/ 2 replaceable GARDs™
Na270 Sodium Eluant, pH 2.80, case of 4 (950 mL/bottle) Native Sample Standard with Norleucine in Lithium Citrate
11006P
Buffer, 0.25 µmole/mL, each (5 mL/bottle)
Na740 Sodium Eluant, pH 7.40, case of 4 (950 mL/bottle)
SP100 SERAPREP™, each (250 mL/bottle)
TRIONE® Ninhydrin Reagent (4-month* shelf life),
T100C UP100 URIPREP™, each (250 mL/bottle)
case of 4 (950 mL/bottle)
RG011 Sodium Column Regenerant, each (950 mL/bottle) 0352-0033 Kit identical to AT33SP with T200 Replacing T100C:

Na220 Sodium Diluent, pH 2.20, case of 4 (250 mL/bottle) TRIONE® Two-part Ninhydrin Reagent (12-month* shelf life
T200
before mixing), to prepare case of 4 (900 mL/bottle)
Oxidized Feed Hydrolysate Standard in Sodium Citrate
1700-0155 AO33SP Kit Identical to AT33SP with OPA Replacing T100C:
Buffer, 0.25 µmole/mL, each (5 mL/bottle)
0352-0017 Kit Identical to O352-0018 with T200 Replacing T100C: OD104 OPA Diluent, case of 4 (950 mL/bottle)

TRIONE® Two-part Ninhydrin Reagent (12-month* shelf life O120 o-Phthalaldehyde, each (5 g/bottle)
T200
before mixing), to prepare case of 4 (900 mL/bottle)
3700-2000 Thiofluor™, each (10 g/bottle), (2 per kit)
0352-0019 Kit Identical to O352-0018 with OPA Replacing T100C:
High-Efficiency 120-Minute Physiologic Fluid/Native
0352-0015
OD104 OPA Diluent, case of 4 (950 mL/bottle) Sample Kit:

O120 o-Phthalaldehyde, each (5 g/bottle) High-efficiency Lithium Cation-exchange Column,

0354100T
4.0 x 100 mm, includes Amino Acid Test Mixture 1700-0070
3700-2000 Thiofluor™, each (10 g/bottle), (2 per kit)
Cation-exchange GARD™ assembly: Holder
1700-3102
0352-0006 70-Minute Physiologic Fluid/Native Sample Kit: w/ 2 replaceable GARDs™

70-minute High-efficiency Lithium Cation-exchange Li275 Lithium Eluant, pH 2.75, case of 4 (950 mL/bottle)
0354675T Column, 4.6 x 75 mm, includes Amino Acid Test Mixture
1700-0070 Li750 Lithium Eluant, pH 7.50, case of 4 (950 mL/bottle)

53
 Pickering Laboratories Product Catalog

Application Kits

T100C
TRIONE® Ninhydrin Reagent (4-month* shelf life),
case of 4 (950 mL/bottle)
Pesticide & Herbicide Analysis Kits
RG003 Lithium Column Regenerant, each (950 mL/bottle) Pesticide & Herbicide Analysis Kits

Li220 Lithium Diluent, pH 2.36, case of 4 (250 mL/bottle) Catalog No. Description

Native Sample Standard with Norleucine in Lithium Citrate 0352-0002 Carbamate Pesticides Analysis Kit, 23+ Compounds
011006P
Buffer, 0.25 µmole/mL, each (5 mL/bottle)
Carbamate Column, expanded resolution, C8, 4.0 x 250
0840250
SP100 SERAPREP™, each (250 mL/bottle) mm, includes Carbamate Test Mixture 1700-0063

UP100 URIPREP™, each (250 mL/bottle) 18ECG001 Guard Cartridge Holder with 3 guard cartridges

0352-0011 Kit Identical to O352-0015 with T200 Replacing T100C: CB130 Carbamate Hydrolysis Reagent, case of 4 (950 mL/bottle)

TRIONE Two-part Ninhydrin Reagent (12-month* shelf life

® CB910 Carbamate OPA Diluent, case of 4 (950 mL/bottle)
T200
before mixing), to prepare case of 4 (900 mL/bottle)
O120 o-Phthalaldehyde, each (5 g/bottle)
0352-0012 Kit Identical to O352-0015 with OPA Replacing T100C:
3700-2000 Thiofluor™, each (10 g/bottle), (2 per kit)
OD104 OPA Diluent, case of 4 (950 mL/bottle)
1700-0025 ChlorAC Buffer, case of 4 (25 mL/bottle)
O120 o-Phthalaldehyde, each (5 g/bottle)
1700-0195 Carbamate Calibration Starter Kit (2 per pack)
3700-2000 Thiofluor™, each (10 g/bottle), (2 per kit)
0352-0003 Carbamate Pesticides Analysis Kit, for EPA Method 531.1:
Temperature Gradient Physiologic Fluid/Native
0352-0013 Carbamate Column, high resolution/capacity, 4.6 x 250 mm,
Sample Kit: 0846250
includes Carbamate Test Mixture 1700-0063
High-efficiency Lithium Cation-exchange Column,
0354100T 18ECG001 Guard Cartridge Holder with 3 guard cartridges
4.0 x 100 mm, includes Amino Acid Test Mixture 1700-0070
Cation-exchange GARD™ assembly: Holder w/ 2 CB130 Carbamate Hydrolysis Reagent, case of 4 (950 mL/bottle)
1700-3102
replaceable GARDs™
CB910 Carbamate OPA Diluent, case of 4 (950 mL/bottle)
1700-1125 Lithium Eluant, pH 2.80, case of 4 (950 mL/bottle)
O120 o-Phthalaldehyde, each (5 g/bottle)
Li220 Lithium Diluent, pH 2.36, case of 4 (250 mL/bottle)
3700-2000 Thiofluor™, each (10 g/bottle), (2 per kit)
Li365 Lithium Eluant, pH 3.65, case of 4 (950 mL/bottle)
1700-0025 ChlorAC Buffer, case of 4 (25 mL/bottle)
Li375 Lithium Eluant, pH 3.75, case of 4 (950 mL/bottle)
1700-0195 Carbamate Calibration Starter Kit (2 per pack)
RG003 Lithium Column Regenerant, each (950 mL/bottle)
0352-0009 Carbamate Pesticides Analysis Kit, for EPA Method 531.2:
Native Sample Standard without Norleucine &
1700-0170 Alpha-Amino-Beta-Guanidinopropionic Acid in Lithium Carbamate Column, high resolution/capacity, 4.6 x 250 mm,
Citrate Buffer, 0.25 µmole/mL, each (5 mL /bottle) 0846250
includes Carbamate Test Mixture 1700-0063
SP100 SERAPREP™, each (250 mL/bottle) 18ECG001 Guard Cartridge Holder with 3 guard cartridges
UP100 URIPREP™, each (250 mL/bottle) CB130.2 Carbamate Hydrolysis Reagent, case of 4 (950 mL/bottle)
TRIONE® Ninhydrin Reagent (4-month* shelf life), CB910 Carbamate OPA Diluent, case of 4 (950 mL/bottle)
T100C
case of 4 (950 mL/bottle)
O120 o-Phthalaldehyde, each (5 g/bottle)
0352-0014 Kit Identical to O352-0013 with T200 Replacing T100C:
3700-2000 Thiofluor™, each (10 g/bottle), (2 per kit)
TRIONE® Two-part Ninhydrin Reagent (12-month* shelf life
T200
before mixing), to prepare case of 4 (900 mL/bottle) 1700-0195 Carbamate Calibration Starter Kit (2 per pack)
0352-0016 Kit Identical to O352-0013 with OPA Replacing T100C: Carbamate Pesticides Analysis Kit, for AOAC
0352-0004
Method 985.23:
OD104 OPA Diluent, case of 4 (950 mL/bottle)
Analytical Column, rapid analysis/QC, 4.6 x 150 mm,
O120 o-Phthalaldehyde, each (5 g/bottle) 1846150
includes Carbamate Test Mixture 1700-0063
3700-2000 Thiofluor™, each (10 g/bottle), (2 per kit) 18ECG001 Guard Cartridge Holder with 3 guard cartridges
* From date of manufacture CB130 Carbamate Hydrolysis Reagent, case of 4 (950 mL/bottle)

CB910 Carbamate OPA Diluent, case of 4 (950 mL/bottle)

O120 o-Phthalaldehyde, each (5 g/bottle)

3700-2000 Thiofluor™, each (10 g/bottle), (2 per kit)

1700-0025 ChlorAC Buffer, case of 4 (25 mL/bottle)

1700-0195 Carbamate Calibration Starter Kit (2 per pack)

54
Pickering Laboratories Product Catalog

Application Kits

(Continued) Pesticide & Herbicide Analysis Kits

Glyphosate Herbicide Analysis Kit, for EPA Method 547, 0352-0053 Multi-Residue Mycotoxin Kit:
0352-0010
AOAC Method 991.08:
1612124 MYCOTOX™ Reversed-phase Column, 4.6 x 250 mm
Cation-exchange Column for Glyphosate analysis, 4 x 150 mm,
1954150
including Glyphosate Test Mixture 1700-0080 18ECG001 Guard Cartridge Holder with 3 cartridges
Cation-exchange GARD™ assembly: Holder w/ 2 OD104 OPA Diluent, case of 4 (950 mL/bottle)
1700-3102
replaceable GARDs™
Glyphosate Potassium Phosphate Eluant, case of O120 o-Phthalaldehyde, each (5 g/bottle)
K200
4 (950 mL/bottle) Sodium Phosphate Eluant, pH 3.3, case
1700-1108
of 4 (950 mL/bottle)
RG019 Glyphosate Column Regenerant, each (950 mL/bottle)
3700-2000 Thiofluor™, each (10 g/bottle), (2 per kit)
GA104 Glyphosate OPA Diluent, case of 4 (950 mL/bottle)
0352-0050 Aflatoxin Analysis Kit:
GA116 Glyphosate Hypochlorite Diluent, case of 4 (950 mL/bottle)

O120 o-Phthalaldehyde, each (5 g/bottle) 1612124 MYCOTOX™ Reversed-phase Column, 4.6 x 250 mm

3700-2000 Thiofluor™, each (10 g/bottle), (2 per kit) 18ECG001 Guard Cartridge Holder with 3 cartridges

1700-0140 RESTORE , each (250 mL/bottle)

™ 0352-0051 Polyether Antibiotics Kit:

2381750 Polyether Reversed-phase Column, 4.6 x 250 mm

Other Post-Column Analysis Kits

18ECG001 Guard Cartridge Holder with 3 cartridges

3700-2200 Vanillin, 30 g (2 per kit)

Other Post-Column Analysis Kits

Catalog No. Description

0352-0040 Biogenic Amines/Polyamines Analysis Kit:

ALKION™ Cation-exchange Column, K+ form,
9410917
4.0 x 150 mm
9493020 ALKION™ Guard Column, K+ form, 3.0 x 20 mm
Flow Restrictor, PEEK Tubing, 0.005" ID, 550 psi @
1100-0161
0.3 mL/min in 15% Methanol in Water
Potassium Phosphate Eluant, pH 5.63,
K563
case of 4 (950 mL/bottle)
Potassium Phosphate Eluant, pH 6.00,
K600
case of 4 (950 mL/bottle)
K130 Potassium Column Regenerant, each (950 mL/bottle)

OD104 OPA Diluent, case of 4 (950 mL/bottle)

O120 o-Phthalaldehyde, each (5 g/bottle)

3700-2000 Thiofluor™, each (10 g/bottle), (2 per kit)

0352-0041 Aminoglycoside Antibiotics Analysis Kit:

9410917 ALKION™ Cation-exchange Column, K+ form, 4.0 x 150 mm

9493020 ALKION™ Guard Column, K+ form, 3.0 x 20 mm

Flow Restrictor, PEEK Tubing, 0.005" ID, 550 psi @
1100-0161
0.3 mL/min in 15% Methanol in Water
Potassium Phosphate Eluant, KO1,
1700-1101
case of 4 (950 mL/bottle)
Potassium Hydroxide Eluant, KO2,
1700-1102
case of 4 (950 mL/bottle)
1700-1103 Potassium Chloride Eluant, KO3, case of 4 (950 mL/bottle)

OD104 OPA Diluent, case of 4 (950 mL/bottle)

O120 o-Phthalaldehyde, each (5 g/bottle)

3700-2000 Thiofluor™, each (10 g/bottle), (2 per kit)

55
 Pickering Laboratories Product Catalog

Replacement Components, Fittings & Tubing

Onyx PCX Flow Diagram – Two-Pump System

Nut: 1452-0113
Ferrule: 1452-0115

FLUSH
TRANSDUCER 2

R2

R1
Nut: 1452-0118
1552-0017
Ferrule: 1452-0117
Nut: 1452-0180
COLUMN 1452-0184 Ferrule: 1452-0181

1 1452-0188
2 6
1452-0189 2102-0463
Nut: 1452-0116 PUMP 2
Ferrule: 1452-0115 1452-0093
3 VALVE 2 5 2103-0463
4
3102-9040 1452-0185
FILTER
1452-0041

1452-0183

Nut: 1452-0118 1100-2927

Ferrule: 1452-0117 HEATED AMBIENT
REACTOR REACTOR

Nut: 1452-0113
Ferrule: 1452-0115
Nut: 1452-0118
Ferrule: 1452-0117 TRANSDUCER 1
1552-0017
1452-0040
1452-0188

1452-0189 Nut: 1452-0114

Ferrule: 1452-0112
3102-9040 FILTER 1452-0184 1
Coupler: 3102-3064 2 6

Nut: 1452-0113
1452-0093 Ferrule: 1452-0115
2102-0463 PUMP 1
3 VALVE 1 5
4
Nut: 1452-0118
Ferrule: 1452-0117
Nut: 1452-0118
Nut: 3101-0011 Ferrule: 1452-0117
Ferrule: 1452-0112 WASTE 1452-0185 Union: 3102-3063
Back Pressure
Check Valve: 3106-1007 TO DETECTOR
Regulator: 3102-9025
Nut: 1452-0226
Ferrule: 1452-0112
FROM Nut: 1452-0116
HPLC Ferrule: 1452-0115

Bold = Kit

56
Pickering Laboratories Product Catalog

Replacement Components, Fittings & Tubing

Onyx PCX Flow Diagram – One-Pump System

FLUSH
R1

Nut: 1452-0118
Ferrule: 1452-0117 Nut: 1452-0180
Ferrule: 1452-0181

1452-0182

Nut: 1452-0118
Ferrule: 1452-0117

1452-0040
2103-0463

COLUMN FILTER 3102-9040

2102-0463

HEATED
REACTOR
1452-0187
TRANSDUCER

1452-0190

1552-0017 Nut: 1452-0113 Nut: 1452-0114

Ferrule: 1452-0115 Ferrule: 1452-0112
1
2 6
1452-0184
Coupler: 3102-3064 Nut: 1452-0113
1452-0093 Ferrule: 1452-0115
3 VALVE 1 5
Nut: 1452-0116 PUMP 4
Ferrule: 1452-0115

2102-0463
Nut: 1452-0118
Ferrule: 1452-0117
Nut: 1452-0118
Nut: 3101-0011 Ferrule: 1452-0117
Ferrule: 1452-0112 WASTE
1452-0185 Union: 3102-3063
Check Valve: 3106-1007 TO DETECTOR Back Pressure
Regulator: 3102-9025
Nut: 1452-0226
Ferrule: 1452-0112
FROM
HPLC

Bold = Kit

57
 Pickering Laboratories Product Catalog

Replacement Components, Fittings & Tubing

Vector PCX Flow Diagram

Flow Path Diagram

HEATED
REACTOR
from
HPLC Column
A
2104-0210
(PEEK Tubing)
3102-9040
(Filter Frit)
1452-0040
(Liquid Manifold) 1452-0041
A (Filter Manifold)
3102-9040 E
(Filter Frit) E
500 psi Over
Pressure
Relief Valve

RESTRICTOR RESTRICTOR
B

AMBIENT 1100-2927
REACTOR

E
0.5 µm filter 0.5 µm filter
3102-9042 A 3102-9042

E E A
to
Detector

PULSE PULSE
3106-1256 C
DAMPENER
3106-1256
DAMPENER
(Pulse Dampener (Pulse Dampener
Rebuild Kit) Rebuild Kit)
E E
to Injector
A
E
3106-1254
3106-1255
1552-0037 425 psi B PUMP 1 3106-1257 PUMP 2
(Pressure Switch) 3106-1259
(Vector Pump
E
Components)
from
Pump D F D D F

2103-0463

Reagent Reservoir & Cap Assemblies

BOTTLE BOTTLE

A Nut: 1452-0118 C Nut: 1452-0177 E 2104-0220

Ferrule: 1452-0117 Ferrule: 1452-0176 (PEEK Tubing,
0.02” ID)
B Nut: 1452-0179 D Nut: 1452-0180
Ferrule: 1452-0178 Ferrule: 1452-0181 F 3106-1010

58
Pickering Laboratories Product Catalog

Replacement Components, Fittings & Tubing

Onyx PCX Reagent Pump Pinnacle PCX Reagent Pump and Valve Components (Continued)

and Valve Components Catalog No. Description

1452-0349 Syringe Pump Rebuild Kit

Onyx PCX Reagent Pump and Valve Components
1552-0017 PCX Pressure Transducer Assembly
Catalog No. Description
Pinnacle PCX Dead-head Kit for Transducer for
1552-0040
1000-1003 Single Syringe Pump PM Kit Chromium VI and Alprostadil Systems

1000-1004 Dual Syringe Pump PM Kit

Vector PCX Reagent Pump

1100-0550 Onyx PCX Tubing Kit - Dual Pump System

1100-0551 Onyx PCX Tubing Kit - Single Pump System

1352-0007 Syringe Pump Cylinder, Ceramic 70 mL Components

1352-0019 Syringe Pump PEEK® Face
Vector PCX Reagent Pump Components
1352-0020 Metal Piston Face
Catalog No. Description
1452-0077 Onyx PCX Valve Assembly, Valve Face and Motor
1000-1006 Vector PCX Dual Pump PM Kit
1452-0079 Onyx PCX Pump Assembly, 70 mL with Motor
1452-0235 Check Valve Cartridge, PEEK® (2/pk) Vector
1452-0093 PCX Valve Face Assembly, Liquid End
1452-0332 Syringe Pump PEEK® Cylinder Front Assembly
1452-0122 Syringe Pump Seal Kit
3102-9028 Inline Filter, 0.5 µm Frit
1452-0200 PCX Valve Seal Tool
3106-1254 Pump Check Valve Kit, PEEK® (Inlet & Outlet)
1452-0201 PCX Valve Seals Kit
3106-1255 Seal Kit (Piston seal, Back-up O-ring, Diaphragm, Tool)
1452-0202 PCX Valve Maintenance Kit (Seals and Tools) Pulse Damper Rebuild Kit (Diaphragm, O-rings,
3106-1256
Diaphragm seal, Seal tool, and 4 Hex wrenches)
1452-0332 Syringe Pump PEEK® Cylinder Front Assembly
3106-1257 Piston, Vector Pump
1452-0349 Syringe Pump Rebuild Kit
3106-1258 Prime/Purge Valve, PEEK®
1552-0017 PCX Pressure Tranducer Assembly
Head Kit, Vector Pump, (Head, Check Valves, Piston
PCX Dead-head Kit for Transducer for Chromium VI and 3106-1259
1552-0040 and Seal)
Alprostadil Systems
3106-1260 Prime Purge Seal Kit, PEEK®

3106-1266 Piston Retainer Bushing

Pinnacle PCX Reagent Pump

and Valve Components Onyx PCX Replacement Reactors
Pinnacle PCX Reagent Pump and Valve Components
Onyx PCX Replacement Reactor Volume Cartridge Coils
Catalog No. Description
Catalog No. Description
1000-1003 Single Syringe Pump PM Kit
1452-0221 Reactor Cooling Coil, Voglibose
1000-1004 Dual Syringe Pump PM Kit
1452-0241 Onyx Reactor Heater Assembly
1100-0500 Pinnacle PCX Tubing Kit - Dual Pump System
1452-0363 Onyx Reactor Cartridge Coil Assembly - 0.15 mL 130 °C
1100-0501 Pinnacle PCX Tubing Kit - Single Pump System
1452-0364 Onyx Reactor Cartridge Coil Assembly - 0.5 mL 130 °C
1352-0007 Syringe Pump Cylinder, Ceramic 70 mL
1452-0365 Onyx Reactor Cartridge Knitted Assembly - 1.4 mL 130 °C
1352-0019 Syringe Pump PEEK® Face
1452-0366 Onyx Reactor Cartridge Knitted Assembly - 2.0 mL 80 °C
1352-0020 Metal Piston Face
1452-0367 Onyx Reactor Cartridge Knitted Assembly - 3.0 mL 80 °C
1452-0038 Pinnacle Syringe Pump Assembly, 70 mL with Motor
1452-0368 Onyx Reactor Cartridge Coil Assembly - 3.5 mL 130 °C
1452-0045 Pinnacle PCX Valve Assembly, Valve Face and Motor
Onyx Reactor Cartridge Knitted Assembly - 1.2 mL &
1452-0369
1452-0093 PCX Valve Face Assembly, Liquid End 1.6 mL 130 °C

1452-0122 Syringe Pump Seal Kit 1452-0370 Onyx Reactor Cartridge Knitted Assembly - 1.0 mL

1452-0200 PCX Valve Seal Tool 1452-0359 Calibration Reactor

1452-0201 PCX Valve Seals Kit

1452-0202 PCX Valve Maintenance Kit (Seals and Tools)

1452-0332 Syringe Pump PEEK® Cylinder Front Assembly

59
 Pickering Laboratories Product Catalog

Replacement Components, Fittings & Tubing

Pinnacle PCX Vector PCX Substitute Reactor Volumes

or Replacement Reactors (Continued)
Replacement Reactors Catalog No. Description
Reactor Heater & Knitted Assembly, 3.5 mL,
Pinnacle PCX Replacement Reactor Volume Cartridge Coils 1452-0223
130 °C max, 120 V
Catalog No. Description Reactor Heater & Knitted Assembly, 3.5 mL,
1452-0224
130 °C max, 240 V
1452-0063 Pinnacle Reactor Heater Assembly
Reactor Heater & Knitted Assembly, 1.0 mL,
1452-0325
1452-0064 Reactor Cartridge Coil Assembly - 0.5 mL 130 °C 130 °C max, 120 V

1452-0094 Reactor Cartridge Coil Assembly - 0.15 mL 130 °C Reactor Heater & Knitted Assembly, 1.0 mL,
1452-0326
130 °C max, 240 V
1452-0096 Reactor Cartridge Knitted Assembly - 1.4 mL 130 °C

1452-0097 Reactor Cartridge Knitted Assembly - 2.0 mL 80 °C

1452-0098 Reactor Cartridge Knitted Assembly - 2.8 mL 80 °C Reagent Reservoirs &

1452-0099 Reactor Cartridge Knitted Assembly - 3.0 mL 80 °C
Cap Assemblies
1452-0100 Reactor Cartridge Knitted Assembly - 1.2 mL & 1.6 mL 130 °C

1452-0221 Reactor Cooling Coil, Voglibose Reagent Reservoirs & Cap Assemblies

1452-0225 Reactor Cartridge Coil Assembly - 3.5 mL 130 °C Catalog No. Description

1452-0324 Reactor Cartridge Knitted Assembly - 1.0 mL Bottle, Piston Wash with Cap, Onyx, Pinnacle,
1452-0120
& Vector PCX
1452-0121 Bottle, System Flush with Cap, Pinnacle/Onyx PCX

Vector PCX Substitute 1452-0356 Reagent & Gas Line Clip, pack of 2

Reactor Volumes or 1925-0129 Cap for Flush Bottle, GL-38, Black, Pinnacle/Onyx PCX
Cap for Wash Bottle, GL-38, Black, Onyx, Pinnacle,
Replacement Reactors 1925-0130
& Vector PCX
3107-0137 Reservoir Bottle, Safety-coated, 1 L
Vector PCX Substitute Reactor Volumes or Replacement Reactors Reservoir Cap for Storage of 1 L and 2 L Bottles,
3107-0138
(No Valve)
Catalog No. Description
3107-0145 Reservoir Bottle, Safety-coated, 2 L
1100-2927 OPA Ambient Reactor, 0.010 in ID TFE Tubing
Cap Assembly for 1 L Bottle, includes Cap with
Reactor Heater & Coil Assembly, 0.15 mL, 3107-0147
1452-0160 Integrated Valve
130 °C max, 120 V
Cap Assembly for 2 L Bottle, includes Cap with
Reactor Heater & Coil Assembly, 0.15 mL, 3107-0148
1452-0161 Integrated Valve
130 °C max, 240 V
Reservoir Assembly, includes 1 L Bottle, and Cap with
Reactor Heater & Coil Assembly, 0.5 mL, 3107-0300
1452-0162 Integrated Valve
130 °C max, 120 V
Reactor Heater & Coil Assembly, 0.5 mL,
1452-0163
130 °C max, 240 V

1452-0166
Reactor Heater & Knitted Assembly, 1.4 mL, Pressure Regulators,
Valves & Gauges
130 °C max, 120 V
Reactor Heater & Knitted Assembly, 1.4 mL,
1452-0167
130 °C max, 240 V
Pressure Regulators, Valves & Gauges
Reactor Heater & Knitted Assembly, 2.0 mL,
1452-0168
130 °C max, 120 V Catalog No. Description
Reactor Heater & Knitted Assembly, 2.0 mL,
1452-0169 1452-0141 Gas Manifold Assembly, PCX
130 °C max, 240 V
Reactor Heater & Knitted Assembly, 2.8 mL, 1452-0343 Over Pressure Relief Valve Cartridge
1452-0170
130 °C max, 120 V
3102-9010 Back Pressure Regulator 1000 psi
Reactor Heater & Knitted Assembly, 2.8 mL,
1452-0171 Pressure Regulator, 100 psi, 1/4-28 connection, for detec-
130 °C max, 240 V 3102-9025
tor waste, PCX
Reactor Heater & Knitted Assembly, 3.0 mL,
1452-0172 3106-1007 Inline Check Valve, PCX
130 °C max, 120 V
Reactor Heater & Knitted Assembly, 3.0 mL, 3106-1010 Check Valve, Reagent Cap
1452-0173
130 °C max, 240 V
Reactor Heater & Knitted Assembly, 1.2 & 1.6 mL,
1452-0174
130 °C max, 120 V
Reactor Heater & Knitted Assembly, 1.2 & 1.6 mL,
1452-0175
130 °C max, 240 V

60
Pickering Laboratories Product Catalog

Replacement Components, Fittings & Tubing

Tubing, Plastic
Tubing, Plastic

Catalog No. Description

2101-0212 TFE Tubing, 1/16" OD x 0.010" ID, 90 cm

2101-0216 TFE Tubing, 1/16" OD x 0.016" ID, 90 cm

2101-0220 TFE Tubing, 1/16" OD x 0.020" ID, 90 cm

2101-0225 TFE Tubing, 1/16" OD x 0.025" ID, 90 cm

2101-0232 C-Flex Tubing, 1/4" OD x 1/8" ID, 90 cm

2102-0463 FEP Tubing, 1/8" OD x 0.063" ID, 90 cm

Flow Restrictors 2103-0463 Air Barrier Tubing, 1/8" OD x 0.063" ID, 90 cm

2104-0210 Inert PEEK® Tubing, 1/16" OD x 0.010" ID, 90 cm

Flow Restrictors 2104-0220 Inert PEEK® Tubing, 1/16" OD x 0.020" ID, 90 cm
Catalog No. Description
Flow Restrictor, TRIONE® Ninhydrin Reagent,
1100-0141
Fittings, Plastic
65 psi @ 0.3 mL/min, 6 cm
1100-0142 Flow Restrictor, 100 psi @ 0.3 mL/min, 10 cm
Flow Restrictor, PEEK® Tubing, 0.005" ID, 550 psi @ Fittings, Plastic
1100-0161
0.3 mL/min in 15% Methanol in Water
Catalog No. Description
1100-0205 Flow Restrictor, 200 psi @ 0.3 mL/min, 6 cm
1452-0112 Ferrule, 1/16 x 1/4-28 (PEEK®), Pinnacle PCX - Pack of 5
1100-0262 Flow Restrictor, 1150 psi @ 0.3 mL/min, 20 cm
1452-0113 Nut, Short, 1/8 x 1/4-28 (PEEK®), Pinnacle PCX - Pack of 5
1100-0263 Flow Restrictor, 550 psi @ 0.3 mL/min, 17 cm
1452-0114 Nut, Short, 1/16 x 1/4-28 (PEEK®), Pinnacle PCX - Pack of 5

1452-0115 Ferrule, 1/8 x 1/4-28 (PEEK®), Pinnacle PCX - Pack of 5

Filters & Frits 1452-0116 Nut, Long, 1/8 x 1/4-28 (PEEK®), Pinnacle PCX - Pack of 5

Filters & Frits 1452-0117 Ferrule, 1/16 (PEEK®), Lite Touch - Pack of 5

Catalog No. Description 1452-0118 Nut, 1/16 (PEEK®), Lite Touch - Pack of 5

1452-0330 Vector 0.5um filter Upgrade Kit 1452-0176 Ferrule, PEEK® 1/16 x 10-32 S/T, 5 each

3102-9040 Replacement Reagent 10 µm Frit 1452-0177 Nut, PEEK® Long 1/16 x 10-32 S/T, 5 each

3102-9042 Replacement Reagent 0.5 µm Frit 1452-0180 Nut, 1/4-28, 1/8" (Polypropylene) - Pack of 5

1452-0181 Ferrule, 1/4-28, 1/8" (Tefzel) - Pack of 5

1452-0226 Nut, Long, 1/16 x 1/4-28 (PEEK®), Pinnacle PCX - Pack of 5

Tees & Unions 3101-0011 Nut, Female PEEK® 1/4-28

Tees & Unions 3101-0020 Plug (Male), 1/4-28 (Delrin)

Catalog No. Description 3101-0030 Plug (Male), 10-32, 1/16" (Delrin)

1452-0040 Liquid Manifold Assembly with Relief Valve, PCX 3102-9160 Removal Tool, for Lite-Touch Ferrule

1452-0041 Liquid “T” Manifold Assembly, PCX

Fittings, Type 316 Stainless Steel

Fittings, Type 316 Stainless Steel

Catalog No. Description

1452-0178 Ferrule, Parker type, 1/16" - Pack of 5

1452-0179 Nut (Male), Parker type, 10-32, 1/16" - Pack of 5

61
 Pickering Laboratories Product Catalog

Product Testing Solutions

Artificial Perspiration

Artificial Eccrine Perspiration Pickering Laboratories created our Artificial Eccrine

Perspiration to universally standardize across all
The Artificial Eccrine Perspiration we offer is a industries; it is the only formula that can satisfy all
ready-to-use solution and the closest mimic to test challenges. Although it is the most complete
true human eccrine sweat. It consists of 19 amino formulation available, we also offer industry-specific
acids, the seven most abundant minerals, and the artificial perspiration formulations.
four most abundant metabolites at a pH of 4.5.
All concentrations closely match experimentally
determined values for adult human eccrine sweat. Artificial Sebum
Custom formulations are available on request. Sebum is an oily secretion produced by sebaceous
glands, which spreads over the hair and skin for
Artificial Eccrine Perspiration List of Ingredients
waterproofing purposes. Pickering Laboratories man-
Uric Acid Urea
Metabolites ufactures an artificial sebum formulation according
Lactic Acid Ammonia
to ASTM designation D4265-14 or D4265-98. It is
Sodium Iron ready-to-use and provides the reliability, reproduc-
Minerals
Calcium Copper Sulfate ibility and convenience needed for testing.
Magnesium Potassium Nitrate
Zinc Chloride D4265-14 Artificial Sebum

L-Serine (Largest Catalog No. Description

Glycine L-Histidine
amount)
Artificial Sebum, ASTM D4265-14, Not Stabilized, each
L-Alanine L-Isoleucine L-Threonine 1700-0700
(25 g/bottle)
L-Arginine L-Leucine L-Tyrosine Artificial Sebum, ASTM D4265-14, Not Stabilized, each
1700-0702
Amino Acids (200 g/bottle)
L-Asparagine L-Lysine L-Valine
L-Aspartic acid L-Methionine Taurine
L-Citrulline L-Ornithine
L-Glutamic acid L-Phenylalanine
Eccrine Perspiration-
Sebum Emulsion
Pickering Laboratories is pleased to offer our Artificial Inspired by the ASTM D4265-98 method for staining,
Eccrine Perspiration in a larger format – in addition Eccrine Perspiration is emulsified with Artificial Sebum.
to the 200 mL and 5 mL quantities, we now offer our Prepared without dust/dirt for a more universal
stabilized formulation in a 950 mL volume, packaged application, this emulsion mimics non-exercise
in a 1 L plastic eluant bottle. induced skin surface f ilm liquids (SSFL). As SSFL,
it can be used to test any topical use product or the
Storage and Handling stability of any article that will come in contact with
The stabilized solution is preserved with a fungicide human sweat. This product requires refrigeration to
and bactericide. The non-stabilized product is kept remain in solution and prevent rancidity.
frozen. Eccrine Perspiration-Sebum Emulsion

Artificial Eccrine Perspiration Catalog No. Description

Catalog No. Description Artificial Eccrine Sweat-Sebum Emulsion, each

1700-0547
(250 mL/bottle)
Artificial Eccrine Perspiration – Not Stabilized, each
1700-0022 Artificial Eccrine Sweat-Sebum Emulsion, each
(200 mL/bottle) 1700-0549
(950 mL/bottle)
Artificial Eccrine Perspiration – Stabilized, each
1700-0020
(200 mL/bottle)
Artificial Eccrine Perspiration – Custom pH,
1700-0023
Not Stabilized, each (200 mL /bottle)
Artificial Eccrine Perspiration – Custom pH, Stabilized,
1700-0021
each (200 mL/bottle)
Artificial Eccrine Perspiration – Stabilized,
1700-0024
each (5 mL/bottle)
Artificial Eccrine Perspiration – Stabilized,
1700-0531
each (950 mL/bottle)

62
Pickering Laboratories Product Catalog

Product Testing Solutions

Artificial Perspiration
Continued

Artificial Apocrine Perspiration

Apocrine sweat is secreted by apocrine glands located
in the areas of the body with an abundance of hair
follicles such as the scalp, armpits and groins. Apocrine
sweat is initially sterile and odorless but when acted
upon by bacteria, it forms odorous compounds. Artifi-
cial Apocrine Perspiration was developed to mimic
the composition of human apocrine sweat and con-
tains several volatile fatty acids that are responsible
for the unpleasant odor associated with it.
The ready-to-use solution is stored frozen and could
be used for testing that requires the presence of mal-
odor. It also could be used to culture bacteria that are AATCC Test Method 15
commonly present on human skin.
Artificial Perspiration
Artificial Apocrine Perspiration List of Ingredients
Urea Citric Acid Butyric Acid
Colorfastness to Perspiration for Fabric
2-hydroxybuteric This ready-to-use solution is used to determine the
Ammonia i-Valeric Acid
Acid fastness of colored textiles to the effects of acid per-
Metabolites 3-hydroxybuteric spiration. The non-stabilized formulation at pH 4.3 is
Lactic Acid
Acid
kept frozen.
a-hydroxy-
Formic Acid (Custom pH and stabilized versions available)
isobutyric Acid
Sugars Glucose
AATCC Test Method 15 Artificial Perspiration
Alanine Aspartic Acid Citruline
Catalog No. Description
Glutamic Acid Glutamine Glycine Artificial Perspiration, AATCC TM 15, Not Stabilized,
1700-0012
each (200 mL/bottle)
Lysine
Amino Acids Isoleucine Leucine
Monohydrochloride Artificial Perspiration, AATCC TM 15, Stabilized,
1700-0015
each (200 mL/bottle)
Phenylalanine Proline Serine
Artificial Perspiration, AATCC TM 15, Custom pH,
Threonine Tryptophan Tyrosine 1700-0527
Not Stabilized, each (200 mL /bottle)
Valine Creatine
Artificial Perspiration, AATCC TM 15, Custom pH,
1700-0528
Sodium Iron Nitrate Stabilized, each (200 mL /bottle)
Calcium Copper Sulfate Artificial Perspiration, AATCC TM 15, Stabilized,
Minerals 1700-0541
case of 4 (950 mL/bottle)
Magnesium Potassium
Artificial Perspiration, AATCC TM 15, Stabilized,
Zinc Chloride 1700-0555
(19.8 L Carboy)
Free Fatty Palmitic Acid Stearic Acid Oleic Acid
Acids Linoleic Acid

ANSI-BHMA A156.18
Triglycerides
Other Cholesterol Squalene
of Fatty Acids
Components
Wax Esters
Artificial Perspiration
Artificial Apocrine Perspiration
For Testing Builders Hardware and Finishes
Catalog No. Description
This ready-to-use solution is used in the test method
specified by the builder’s hardware association
1700-0556 Artificial Apocrine Perspiration, each (250 mL/bottle)
(Custom pH version available)

ANSI-BHMA A156.189 Artificial Perspiration

Catalog No. Description

Artificial Perspiration, ANSI-BHMA A156.18,

1700-0504
Not Stabilized, each (200 mL /bottle)
Artificial Perspiration, ANSI-BHMA A156.18, Custom pH,
1700-0512
Not Stabilized, each (200 mL/bottle)

63
 Pickering Laboratories Product Catalog

Product Testing Solutions

Artificial Perspiration
Continued

BS EN 1811:2011 Artificial GMW14334 Artificial Perspiration

Perspiration Tests the chemical resistance of automotive trim
materials and components. Acid and alkaline per-
For Release of Nickel spiration solutions are formulated according to test
This ready-to-use solution is used in the test method procedure GMW14334.
for release of Nickel from all post assembles which GMW14334 Artificial Perspiration
are inserted into pierced parts of the human body
Catalog No. Description
and articles intended to come into direct and pro-
longed contact with skin. The non-stabilized version 1700-0533
GMW14334 Artificial Perspiration, Acidic,
each (200 mL /bottle)
is kept frozen.
GMW14334 Artificial Perspiration, 2-Part Alkaline,
1700-0534
This formulation is also chemically equivalent to each (200 mL /bottle)
method DIN 53160-22010-10 with the exception of
titration by Sodium Hydroxide. Method DIN 53160 is
used to determine fabric colorfastness to perspiration. ISO 105-B07 and ISO 105-E04
(Custom pH and stabilized versions available) Artificial Perspiration
BS EN 1811:2011 Artificial Perspiration
Colorfastness to Light for Fabric
Catalog No. Description
These are ready-to-use solutions that are used to test
Artificial Perspiration, BS EN 1811:2011, Not Stabilized,
1700-0009
each (200 mL/bottle)
the colorfastness to light of a fabric saturated with
either the acidic or alkaline solution. The pH of the
Artificial Perspiration, BS EN 1811:2011, Stabilized,
1700-0506
each (200 mL/bottle) acidic solution is 5.5 and that of the alkaline solution
Artificial Perspiration, BS EN 1811:2011, Custom pH, is 8.0. The non-stabilized product is kept frozen.
1700-0521
Not Stabilized, each (200 mL /bottle) (Custom pH and stabilized versions available)
Artificial Perspiration, BS EN 1811:2011, Custom pH,
1700-0515
Stabilized, each (200 mL /bottle) ISO 105-B07 and ISO 105-E04 Artificial Perspiration
Artificial Perspiration, BS EN 1811:2011, Stabilized,
1700-0566 Catalog No. Description
(19.8 L Carboy)
Artificial Perspiration, ISO 105-B07/105-E04, Acidic,
1700-0010
Not Stabilized, each (200 mL /bottle)

DIN-EN/IEC 60068-2-70 1700-0507

Artificial Perspiration, ISO 105-B07/105-E04, Acidic,
Stabilized, each (200 mL /bottle)

Artificial Perspiration 1700-0516

Artificial Perspiration, ISO 105-B07/105-E04, Acidic,
Custom pH, Not Stabilized, each (200 mL/bottle)

For Testing Resistance of Lettering & 1700-0522

Artificial Perspiration, ISO 105-B07/105-E04, Acidic,
Custom pH, Stabilized, each (200 mL/bottle)
Markings to Abrasion Artificial Perspiration, ISO 105-B07/105-E04, Alkaline,
1700-0011
This ready-to-use solution is intended for testing Not Stabilized, each (200 mL /bottle)

of resistance of lettering and markings to abrasion. 1700-0508

Artificial Perspiration, ISO 105-B07/105-E04, Alkaline,
Stabilized, each (200 mL /bottle)
Non-stabilized version is kept frozen.
Artificial Perspiration, ISO 105-B07/105-E04, Alkaline,
1700-0517
Custom pH, Not Stabilized, each (200 mL/bottle)
DIN-EN/IEC 60068-2-70 Artificial Perspiration
Artificial Perspiration, ISO 105-B07/105-E04, Alkaline,
Catalog No. Description 1700-0523
Custom pH, Stabilized, each (200 mL/bottle)
DIN-EN/IEC 60068-2-70 Artificial Perspiration,
1700-0542
Not Stabilized, each (200 mL /bottle)
DIN-EN/IEC 60068-2-70 Artificial Perspiration,
1700-0543
Stabilized, each (200 mL /bottle)

64
Pickering Laboratories Product Catalog

Product Testing Solutions

Artificial Perspiration
Continued

ISO 11641 Artificial Perspiration ISO 3160 Artificial Perspiration

Colorfastness to Perspiration for Leather Corrosion Resistance for Alloys
This ready-to-use solution is used to determine the This is a ready-to-use solution that is used to determine
fastness of leather to perspiration. The non-stabilized corrosion resistance. The non-stabilized formulation
formulation at pH 8.0 and is kept frozen. is at pH 4.7 and is kept frozen.
(Custom pH and stabilized versions available) (Custom pH and stabilized versions available)

ISO 11641 Artificial Perspiration ISO 3160 Artificial Perspiration

Catalog No. Description Catalog No. Description

Artificial Perspiration, ISO 11641, Not Stabilized, Artificial Perspiration, ISO 3160, Not Stabilized,
1700-0013 1700-0026
each (200 mL/bottle) each (200 mL/bottle)
Artificial Perspiration, ISO 11641, Stabilized, Artificial Perspiration, ISO 3160, Stabilized,
1700-0509 1700-0511
each (200 mL/bottle) each (200 mL/bottle)
Artificial Perspiration, ISO 11641, Custom pH, Artificial Perspiration, ISO 3160, Custom pH,
1700-0518 1700-0520
Not Stabilized, each (200 mL /bottle) Not Stabilized, each (200 mL /bottle)
Artificial Perspiration, ISO 11641, Custom pH, Stabilized, Artificial Perspiration, ISO 3160, Custom pH, Stabilized,
1700-0524 1700-0526
each (200 mL /bottle) each (200 mL/bottle)
Artificial Perspiration, ISO 3160, Stabilized,
1700-0532
each (950 mL /bottle)

ISO 12870 Artificial Perspiration 1700-0537

Artificial Perspiration, ISO 3160 with added Pyruvic Acid,
buffered, Stabilized, each (200 mL/bottle)
Artificial Perspiration, ISO 3160, Custom PH, Stabilized,
Ophthalmic Optics 1700-0545
each (950 mL /bottle)

This ready-to-use solution is used to determine the 1700-0557 Artificial Perspiration, ISO 3160, Stabilized, (19.8 L Carboy)
effect of sweat on ophthalmic optics and spectacle
frames. The non-stabilized formulation is kept frozen.
(Custom pH and stabilized versions available) ASTM D2322 Artificial Perspiration
ISO 12870 Artificial Perspiration Resistance of Shoe Upper Leather to
Catalog No. Description
Artificial Perspiration
Artificial Perspiration, ISO 12870, Not Stabilized, This artificial perspiration formulation is specific for
1700-0014
each (200 mL/bottle) breakdown of leather and is used to assess resistance
1700-0510
Artificial Perspiration, ISO 12870, Stabilized, to grain cracking and area loss of shoe upper. The
each (200 mL/bottle) non-stabilized solution has pH 7.5 and should be
1700-0519
Artificial Perspiration, ISO 12870, Custom pH, stored frozen.
Not Stabilized, each (200 mL /bottle)
(Custom pH and stabilized versions available)
Artificial Perspiration, ISO 12870, Custom pH, Stabilized,
1700-0525
each (200 mL /bottle)
ASTM D2322 Artificial Perspiration
Catalog No. Description
1700-0548 ASTM D2322-00 Artificial Perspiration, each
(200 mL/bottle)
ASTM D2322-00 Artificial Perspiration, Stabilized,
1700-0550
each (200 mL/bottle)

65
 Pickering Laboratories Product Catalog

Product Testing Solutions

Artificial Saliva

AFNOR NF S91-141 Artificial Saliva Artificial Saliva for

For Testing Biodegradability of Pharmaceutical Research
Dental Metal Alloys Artificial Saliva is formulated according to literature
Artificial Saliva is prepared according to the AFNOR for pharmaceutical research, such as studies of drug
NF S91-141 standard procedure and is intended for dissolution and drug delivery through oral muco-
testing biodegradability of dental metal alloys. The sa. This is a ready-to-use formulation that should be
formulation consists of two parts that are mixed right stored refrigerated. The pH of the solution is 6.8 +/- 0.1.
before use. This minimizes changes in the solution
during storage and allows for a longer shelf life. The
pH of the solution after mixing is 7.8 +/- 0.1. Artificial
ISO 10271-2011 Artificial Saliva
Saliva should be stored refrigerated both before and Corrosion Testing for Metallic Material
after mixing. Premixed, ready-to-use solution is avail- in Dental Devices
able upon request.
This Artificial Saliva is formulated according to ISO
10271-2011 standard procedure. ISO 10271-2011 speci-
DIN 53160-1:2010-10 Artificial Saliva fies the method for corrosion testing for metallic ma-
terial in dental devices. The solution should be stored
To Determine Colorfastness of Products in the refrigerator and has a 1 year shelf life. The pH of
Intended to be Taken Into the Mouth the solution is 2.3.
Artificial Saliva is prepared according to DIN 53160-
Artificial Saliva
1:2010-10 standard procedure. DIN 53160 specifies
the method to determine colorfastness of products Catalog No. Description
intended to be taken into the mouth. The formula- 1700-0301
Artificial Saliva, Fusayama/Mayer, Not Stabilized,
tion consists of two parts that are mixed right before each (200 mL/bottle)

use. This minimizes changes in the solution during 1700-0306

Artificial Saliva, Fusayama/Mayer, Custom pH,
Stabilized, each (200 mL/bottle)
storage and allows for a longer shelf life. The solution
Artificial Saliva, Fusayama/Mayer, Custom pH,
should be stored refrigerated both before and after 1700-0307
Not Stabilized, each (200 mL/bottle)
mixing. The pH of the solution after mixing is 6.8 +/- 0.1.
1700-0309 Artificial Saliva, Fusayama/Mayer, Stabilized, 200mL
Artificial Saliva, AFNOR NF S91-141, 2-Part, Not Stabilized,
Fusayama/Mayer Artificial Saliva
1700-0302
each (200 mL /bottle)
Artificial Saliva, DIN 53160, 2-Part, Not Stabilized,
1700-0303
each (200 mL/bottle)
For Testing of Products for Corrosion,
Artificial Saliva for pharmaceutical research,
Colorfastness and Discoloration 1700-0304
Not Stabilized, each (200 mL /bottle)
This ready-to-use solution closely resembles the 1700-0308
Artificial Saliva, Pharmaceutical Research, Custom pH
each (200 mL/bottle)
mineral composition of natural saliva and is the most
common media used for testing dental metal alloys. 1700-0305
Artificial Saliva for medical and dental research,
Stabilized, each (200 mL /bottle)
This formulation is at pH 4.9+/- 0.1 and should be
Artificial Saliva for corrosion testing for metallic material
stored refrigerated. 1700-0313 in dental devices, ISO 10271-2011, Not Stabilized, each
(Custom formulations at different pH are available) (200 mL/bottle)
Artificial Saliva, Modified Fusayama/Mayer, Custom pH
1700-0314
up to 6.5, Stabilized, each (200 mL/bottle)

Artificial Saliva for Medical Custom formulations available in pH range 3.0 - 6.0
request a Quote: 800-654-3330 or
and Dental Research orders@pickeringlabs.com

This Artificial Saliva is formulated according to litera-

ture references for medical and dental research. This
formulation has similar composition to commercial-
ly available products used to treat dry mouth and
other conditions. This ready-to-use solution contains
Sodium Carboxymethyl Cellulose to increase viscosity
of the solution and make it behave similar to natural
human saliva. This formulation can be stored at room
temperature and has pH of 6.75 +/- 0.05. This solution
is only intended for product testing.

66
Pickering Laboratories Product Catalog

Product Testing Solutions

Artificial Urine / Other

Artificial Urine for Corrosion

Artificial Urine (Continued)

Catalog No. Description

Testing of Urological Implants, 1700-0600 Artificial Urine, Stabilized, each (200 mL/bottle)

Stabilized 1700-0602 Artificial Urine, Stabilized, each (950 mL/bottle)

This artificial urine is designed for testing metallic 1700-0603 Artificial Urine, DIN EN 1616:1999, Not Stabilized, each (19.8 L)

biomaterials used to produce urological implants and

catheters. This convenient product is a ready to use
solution. The formulation contains non-toxic preser-
Simulated Lung Fluid
vative to avoid bacteria growth and can be stored Gamble’s solution represents the interstitial fluid
at room temperature. The pH of the final solution is deep within the lung and is used to simulate different
6.0+/- 0.4. lung conditions. It is used in pulmonary drug delivery
(Custom formulations are also available)
studies as well as in studies of particles inhalation
effects. Citrate is used in Gamble’s solution instead
of proteins to avoid foaming and acetate instead of
DIN EN 1616:1999 Artificial Urine organic acids. Gamble’s solution has a pH of 7.4. In-
quire about other simulated lung fluid formulations.
For Testing Sterile Urethral Catheters
Simulated Lung Fluid
Artificial Urine is prepared according to DIN EN
1616:1999 standard procedure. DIN EN 1616 specifies Catalog No. Description

the method to test sterile urethral catheters. This 1700-0800

Gamble's Simulated Lung Fluid, Not Stabilized,
each (200 mL/bottle)
ready-to-use solution should be stored frozen to avoid
bacteria growth. The pH of the solution is 6.6.
Artificial Cerumen “Earwax”
Artificial Urine Medium for Cerumen, also known as earwax, is a waxy substance
Growing Urological Pathogens secreted in the ear canal that protects and lubricates
the ear canal and assists in cleaning by trapping dirt
This ready-to-use solution closely resembles com- and dead skin cells. Pickering Laboratories offers
position of human urine and can be used for clinical artificial earwax that can be used for the testing of
studies as well as for product testing. This formulation hearing aids, ear buds and other electronic devices
supports growth of wide range of urinary pathogens meant to be used in the ears.
and it is also capable of forming crystals similar to
these found in natural urinary tract infections. It can Artificial Cerumen “Earwax”

be used as negative controls in laboratory testing. Catalog No. Description

The pH of the Artificial Urine Medium is 6.5. This 1700-0701 Artificial Cerumen, Not Stabilized, each (50 g/bottle)
product is stored frozen.
1700-0711 Artificial Cerumen, Not Stabilized, each (200 g/bottle)

Artificial Urine Stabilized

This ready-to-use solution has the same composition Substitute Ocean Water
as Artificial Urine Medium and closely resembles Substitute Ocean Water is prepared according to
human urine. This formulation contains non-toxic official ASTM method D1141-98. This product could
preservative to avoid bacteria growth and can be be successfully used in a wide variety of tests where
stored at room temperature. The pH of Artificial Urine solution simulating sea water is required, such as oil
Stabilized is 6.5. contamination testing, detergent evaluation and cor-
rosion testing. The pH of the solution is 8.2.
Artificial Urine
The solution is ready-to-use and is stored at room
Catalog No. Description
temperature. Inquire about different sizes.
Artificial Urine for Corrosion Testing of Urological
1700-0016
Implants, Stabilized, each (200 mL/bottle) Substitute Ocean Water
Artificial Urine, DIN EN 1616:1999, Not Stabilized,
1700-0017 Catalog No. Description
each (200 mL/bottle)
Artificial Urine Medium for Growing Urological Substitute Ocean Water, ASTM D1141-98 (2003),
1700-0018 1700-0801
Pathogens, Not Stabilized, each (200 mL/bottle) Not Stabilized, case of 4 (950 mL/bottle)

Artificial Urine, DIN EN 1616:1999, Stabilized, Substitute Ocean Water, ASTM D1141-98 (2003),
1700-0558 1700-0802
each (200 mL/bottle) Stabilized case of 4 (950 mL/bottle)
Substitute Ocean Water, ASTM D1141-98 (2003),
1700-0803
Stabilized, each (200 mL/bottle)

67
 Pickering Laboratories Product Catalog

General Terms & Conditions

Synthetic Hard Water

Hard Water for product testing and dispersibility
testing. Made according to ASTM E1945-02.

Synthetic Hard Water

Catalog No. Description

Synthetic Hard Water, Custom Hardness,
1700-0804
ASTM E1945-02, each (200 mL/bottle)

1 Controlling Document 7 Seller’s Remedies

These T & C’s and only these T & C’s apply to all goods sold and If Buyer fails, with or without cause, to furnish Seller with specifica-
services provided by Seller to Buyer. By the placement of Buyer’s pur- tions and/or instructions for, or refuses to accept deliveries of, any of
chase order, Buyer consents to these T & C’s and no others. the products sold under this contract, or is otherwise in default under
or repudiates this contract or any other contract with Seller or fails to
2 Terms of Payment pay when due any invoice under this contract, then in addition to any
All payments are due and payable thirty (30) days from the date of the and all remedies allowed by law, Seller without notice (1) may deter
invoice. A service charge of 1 1/2 % per month shall apply to all invoices shipment under this or any contract between Buyer and Seller until
not paid within 30 days. such default, breach or repudiation is removed and/or (2) may cancel
any undelivered portion of this and/or any other contract in whole or
in part, Buyer remaining liable for damages.
3 Security Agreement
(a) As security for Buyer’s payment, Buyer grants Seller a purchase 8 Patent Indemnity
money security interest in the specific goods for which payment is
due. Seller has the right to file a financing statement evidencing (a) Seller shall, at its own expense, defend any suit that is instituted
this security interest. against Buyer to the extent such suit alleges that any goods, other
than prototypes, or any part thereof sold or leased hereunder
(b) This security interest shall terminate upon receipt by Seller of pay- infringes on any United States patent, trademark, or copyright
ment for the specific goods. (except goods covered by Section 8(b) below), provided that such
alleged infringement does not arise from any modification or ad-
4 Delivery, Title and Risk of Loss dition to the goods by anyone other than Seller, or the use of such
Title to goods and risk of loss of goods shall pass to Buyer when Seller goods as a part of or in combination with any other device or parts
delivers such goods to a common carrier or Buyer’s agent. Delivery or from the use of such goods to practice any method or process.
shall be EX Works Seller’s Factory. Delivery dates agreed to by Seller Provided further that the Buyer gives Seller immediate notice in
are approximate only. Seller shall not be liable for, nor shall Seller be writing of any such suit and permits Seller, through counsel of its
in breach of its obligations to the Buyer because of any delivery made choice, to answer the charge of infringement and defend such suit;
within a reasonable time after the stated delivery date. Seller may, by and the Buyer gives Seller all the needed information, assistance,
written notice to Buyer, change any delivery date, and such date shall and authority, at Seller’s expense, to enable Seller to defend or
become the agreed upon delivery date unless Buyer objects to such settle such suit. In the case of a final award of damages in any such
date in writing delivered to Seller within ten (10) days of receipt of suit, Seller shall pay such award but shall not be responsible for any
Seller’s notice. settlement made without its prior written consent. In the event
the use, lease, or sale of the goods is enjoined, Seller may at its own
5 Force Majeure option and expense: (1) procure for Buyer the right to use, lease,
or sell such goods, (2) replace such goods, (3) modify such goods,
Seller shall not be liable for any failure to deliver, or delay in the
or (4) remove such goods and refund the purchase price paid by
delivery of, any goods or services due to any cause beyond its control,
Buyer less a reasonable sum for use, damage, and obsolescence.
including but not limited to natural phenomena, government actions,
THIS SECTION STATES SELLER’S TOTAL RESPONSIBILITY AND
fires, labor disputes, or inability to obtain components, energy, materi-
LIABILITY, AND THE BUYER’S SOLE REMEDY FOR ANY ACTUAL
als, manufacturing facilities, or transportation. In the event of such de-
OR ALLEGED INFRINGEMENT OF ANY PATENT, TRADEMARK, OR
lay, the date of delivery or performance hereunder shall be extended
COPYRIGHT BY ANY GOODS DELIVERED HEREUNDER OR ANY
by a period equal to the time lost by reason of such delay. In the event
PART THEREOF. THIS SECTION 8 IS IN LIEU OF AND REPLACES ANY
Seller’s production is curtailed for any of the above reasons, Seller may
OTHER EXPRESS, IMPLIED OR STATUTORY WARRANTY AGAINST
allocate its production to its various Buyers.
INFRINGEMENT.

6 Seller’s Liability (b) The Buyer shall, at its own expense, indemnify and hold Seller
harmless from and against any expense or loss resulting from
If Buyer notifies Seller within 30 days after the date of invoice of a
any infringement of any patent, trademark, or copyright arising
claimed defect, Buyer shall concurrently offer Seller an opportunity
as a result of Seller’s compliance with any of the Buyer’s designs,
to investigate the claim and to inspect allegedly defective goods.
specifications, or instructions, and shall defend at its own expense
Failure to offer Seller such opportunity shall constitute acceptance by
including attorney fees, any suit brought against Seller alleging
Buyer and a waiver of all claims for defects .If Seller determines that
any such infringements provided that Seller (i) gives the Buyer
Buyer’s claim is valid, Seller may repair the defective goods or replace
immediate notice of any such suit and permits the Buyer through
the defective goods with conforming goods at the Seller’s Factory.
counsel of its choice, to defend such suit, and (ii) gives Buyer all
Replacement of defective goods will only be made upon return of the
needed information, assistance, and authority, at the Buyer’s
defective product.
expense, necessary for the Buyer to defend any such suit.

68
Pickering Laboratories Product Catalog

General Terms & Conditions / Distributors

9 Order Cancellation Africa Costa Rica

LC Tech GmbH Orgoma S.A.
Buyer may, at any time, cancel its purchase of goods or
http://www.lctech.de/ http://www.orgoma.com/
services but only by paying 15 % of the purchase price if
such cancellation is received by Seller more than 90 days Argentina Electrónica Centroamérica S.A.
before the scheduled delivery date, 50 % if received more Analytical Technologies http://www.biocientifica.net/
than 45 days but less than 90 days, 75 % if received more http://www.analytical.com/
Analytical Instruments
than 15 days but less than 45 days, and 100 % if received AGS Analitica SA http://www.ai.co.cr/
less than 15 days. http://www.agsanalitica.com/
Egypt
10 Warranty and Limitation of Liability Australia LC Tech GmbH
Walker Scientific http://www.lctech.de/
1) IN NO EVENT SHALL SELLER BE LIABLE FOR ANY LOSS
http://www.walkerscientific.com.au/
PROFITS, INDIRECT, SPECIAL, INCIDENTAL, OR CONSE- El Salvador
QUENTIAL DAMAGES Thermo Fisher Scientific, Inc. Servicios Tecnicos De Ingenieria
RESULTING FROM SELLER’S PERFORMANCE OR http://www.fishersci.com/ (SETISA)
FAILURE TO PERFORM HEREUNDER OR THE FUR- http://www.setisa.com.sv/
Belize
NISHING, PERFORMANCE, OR USE OF ANY GOODS OR Biokim Europe
SERVICES SOLD PURSUANT HERETO.(2) IN NO EVENT http://www.biokimsa.com/ LC Tech GmbH
SHALL THE AMOUNT OF SELLER’S LIABILITY EXCEED http://www.lctech.de/
THE AMOUNTS PAYABLE BY BUYER HEREUNDER. (3) Brazil
IN NO EVENT SHALL SELLER BE LIABLE FOR DAMAG- Chemetric Instrumentação Guatemala
ES RELATING TO ANY INSTRUMENT, EQUIPMENT OR Analítica Ltda Biokim
APPARATUS WITH WHICH THE GOODS SOLD HEREUN- http://www.chemetric.com.br/ http://www.biokimsa.com/
DER ARE USED (4) SELLER EXPRESSLY DISCLAIMS ANY Cromatec do Brasil Comercio de Honduras
LIABILITY FOR ANY WARRANTY, EXPRESS, IMPLIED OR Instrumentos Cientificos Ltda Orgoma S.A.
STATUTORY, INCLUDED BUT NOT LIMITED TO WAR- http://www.cromatec.com.br/ http://www.orgoma.com/
RANTY OF MERCHANTABILITY AND FITNESS FOR A
PARTICULAR PURPOSE, EXCEPT SUCH WARRANTIES Nova Analitica Imp. Exp. Ltda Biokim
EXPRESSLY IDENTIFIED AS WARRANTIES AS ARE SET http://www.analiticaweb.com.br/ http://www.biokimsa.com/
FORTH IN SELLER’S CURRENT OPERATING MANUAL, Agilent – Brazil India
CATALOG OR OTHER WRITTEN GUARANTY COVERING http://www.agilent.com.br/ Spinco Biotech Pvt Ltd
THE PRODUCT. http://www.spincotech.com/
Interprise Instrumentos
Analiticos Ltda. Indonesia
11 General
http://www.interprise.com.br/ P.T. Berca Indonesia
(a) The sale of goods shall be governed by Uniform https://www.berca-indonesia.com/
Canada
Commercial Code and others laws of the State of Cal-
Chromatographic Specialties, Inc. PT. Perkindo Mitra Analitika
ifornia and Santa Clara County, California shall be the
https://www.chromspec.com/ http://www.perkinelmer.com/
appropriate venue and jurisdiction for the resolution of
disputes hereunder. VRW – Canada Israel
https://ca.vwr.com/ Manbar
(b) The Buyer may not assign its rights or obligations
http://www.manbar.co.il/
under this acknowledgment without the prior written Fisher Scientific Canada
consent of Seller, and any purported assignment with- https://www.fishersci.ca/ Korea
out such consent shall have no force or effect. Insung Chroma-Tech Co., Ltd.
Chile
http://www.insung.net/
(c) Any waiver by Seller of any default by the Buyer here- Perkin Elmer Chile Limitada
under shall not be deemed to be a continuing waiver http://www.perkinelmer.com/ YOUNG IN Chromass Co., Ltd.
of such default or a waiver of any other default or any http://www.youngincm.com/
China & Hong Kong Territories
of the terms and conditions of this acknowledgment.
Tegent – China Interface Engineering Co Ltd
(d) These T & C’s may not be superseded, modified, or Http://www.tegent.com.cn/ http://www.interface.co.kr/
amended except in writing stating that there is such
Ameritech Scientific Corporation Laos
a modification and signed by an authorized repre-
http://www.ameritechsc.com.cn/ Fortune Scientific Co., Ltd.
sentative of each party hereto, provided, however that
http://www.fortunesci.co.th/
Seller may modify the specifications of the goods sold Columbia
hereunder if such modification does not change the Casa Cientifica Malaysia
form, fit, or function of such goods. Http://www.casacientifica.com/ IT Technologies Pte Ltd
http://www.ittech.com.sg/
(e) This document constitutes the entire terms and con- Khymos
ditions agreement between the Buyer and Seller with http://www.khymos.com/ Thermo Fisher – Malaysia
regard to the goods or services purchased or provided http://www.fishersci.com.my/
Commonwealth of
and expressly supersedes and replaces any prior or Independent States Lab Alliance Sdn Bhd – Malaysia
contemporaneous agreements, written or oral relating LC Tech GmbH http://laballiance.com.my/
to such goods or services. http://www.lctech.de/
(f) Any lawsuit, sounding either in contract or tort, must
be filed within a year from the date of delivery of the
goods/services to the Buyer.

69
 Pickering Laboratories Product Catalog

Distributors

Mexico Taiwan Vietnam

DIRASA Analab Corporation Tegent
http://www.dirasa.com.mx/ http://www.analab.com.tw/ http://tegent.com.vn/
Rekner – SA. de C.V Vercotech Inc. TRAMAT Co., Ltd
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VWR International S de R.L. de C.V. Inpac Technologies, Inc. Saigon Instrumentation Joint
https://mx.vwr.com/ http://inpac.myweb.hinet.net/ Stock Company (SISC)
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Our dedicated professionals
Alphatech Systems Limited United Staes
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Shimadzu Oceania Pty Ltd. chemistry direct and through
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Biokim Alliance Calibrations Group, LLC

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Perkin Elmer
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IT Technologies Pte Ltd
http://www.ittech.com.sg MG Scientific
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Perkin Elmer – Singapore
http://www.perkinelmer.com/ MLS Technologies
http://www.mlstechnologies.
Thermo Fisher Scientific Pte Ltd
com/
– Singapore
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http://www.shimadzu.com/
Agilent – Singapore
http://www.agilent.com/ Venezuela
Corporacion Cientifica
South Africa
Venezolana, C.A.
Anatech Instruments (Pty) Limited
http://www.ccv.com.ve/
http://anatech.co.za/
Chemetrix Pty Ltd.
http://chemetrix.co.za/
Sri Lanka
Techno Solutions Pvt.Ltd
http://www.technospl.com/

70
 Pickering Laboratories, Inc.
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sales@pickeringlabs.com / support@pickeringlabs.com
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