NEXT GENERATION

SEQUENCING SERVICE
Next-generation sequencing 1 DNA Library Preparation 2 Clonal Amplification
(NGS) technologies have
progressive advantages in 1 2 3
terms of cost-effectiveness,
unprecedented sequencing
speed, high resolution and
accuracy in genomic analy- 
ses, thus are playing an
increasing important role in 4 5
fields of oncology and immu-
nology.

The sequencing principles of

common sequencing instru- 
ments on the market: (1) first, 
construct a DNA template
library. Obtain DNA library
fragments (tens to hundreds
of bases in length) by
randomly breaking genomic
DNA, or construct paired-end
fragments that control the
distance distribution. Adapter
sequences were ligated to
both ends of the DNA 
fragment and then denatured 
to obtain a single-stranded
template library and immobi-
lized on a solid surface. (2) 3 Cyclic Array Sequencing
Second, amplify the clones,
which performed by one of 
 
  
several methods, such as
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3'
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bridge PCR and emulsion 
 
PCR. (3) Then, forming DNA T 

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clusters or amplifying micro-
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spheres of DNA cluster arrays C
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on a chip, performing a series A
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of cyclic reaction operations  
using a polymerase\ or ligase, 
 
and monitoring the optical 
  
events generated in each
cycle biochemical reaction by
a microscopic detection syst-

 

em. Time series analysis of 3' GA CAn n n z z
 
the resulting array images is  zz
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performed to obtain sequenc- GA n CT GT
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es of DNA fragments. These
fragments are then assem- C 3'


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bled into longer contigs G

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according to certain computer T


algorithms.
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Creative Biolabs uses the AAn n n z z z CAn n n z z z CT GT AT TT
advanced SuPrecision™ pl- 3' 
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atform to support researchers GA n n n z z z TAnnn z z z
all over the world with their

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sequencing needs for cancer. 

WHAT WE DO:
Whole Genome Sequencing (WGS)
Creative Biolabs Service for Cancer
Whole Transcriptome Sequencing (WTS)
Service for Cancer
Next Generation Whole Exome Sequencing (WES) Immune Repertoire Sequencing (Rep-seq)
Service for Cancer
Sequencing Service for Cancer
Targeted Sequencing Service for Bioinformatics Analysis Service
Service Cancer

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