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LBYPLNT (General Botany Laboratory)
Group #:___________________ Date of Activity:______________ Professor:_________________ Date of Submission:__________
LABORATORY ACTIVITY 8: CELL CYCLE
I. Introduction
A. Identification of the Interphase and the Stages of Mitosis:
Identify the different stages of the cell cycle described as follows:
1. A large spherical nucleus, with the nuclear membrane intact, grainlike chromosomes and one to two nucleoli. This is the stage of ____________.
2. A large, spherical nucleus with a nucleolus and nuclear membrane intact and with thickened, more distinctly ribbonlike chromosomes. The chromosomes may look like a dish of spaghetti. This is the stage of _______________.
3. A cell in which the chromosomes appear as a loose knot in the center of the cell. The nuclear membrane, if still present, is indistinct. The nucleolus may start to fade. This is the stage of ___________.
4. A cell in which the chromosomes are aligned in the equatorial plane of the cell. This is the stage of ____________.
5. A cell in which the chromatids are moving to opposite poles of the cell. This is the stage of ____________.
6. A cell in which the chromatids, though fairly distinct are close to the opposite poles of the cell. A cell plate may be forming at the middle of the cell. This is the stage of ___________.
7. Look for two cells that appear to have finished dividing recently. These are the daughter cells and the stage seen is the stage of _____________.
C. Determination of Duration of Interphase and the Mitotic Stages
Use Slide images 1 and 2 for this next activity.
1. Estimate the total number of cells in the field of view. Count all the cells in one segment of the field, for example, one quadrant, then multiply this count by the proportion of the total field not counted. For example, if you counted the cells in 1/4 of the field, the total field would contain approximately 4 times this number of cells.
2. Count the number of cells in the recognizable stages of division and add these tallies to the appropriate column in the worksheet. Estimate the number of interphase cells by subtracting the number of cells in all other stages from the total number of cells in the field.
The duration of the cell cycle in a typical onion root tip is about 16 hours. We can estimate the duration of time spent by an individual cell in each stage of mitosis by the proportion of cells in that stage at the instant the tissue was fixed. Use the following formula to calculate the duration of each stage, in minutes.
Duration of stage = number of cells at stage/total number of cells x 16 hrs./cycle x 60
This study source was downloaded by 100000801932233 from CourseHero.com on 05-03-2022 22:41:55 GMT -05:00
https://www.coursehero.com/file/117888393/Activity-8-The-Cell-Cycle-Answer-Sheet-rev2021docx/ The number of cells in your field of view is ___________. Show your calculations below.
Calculate the duration of each stage (min./cell cycle) and enter this into the table below.
Mitotic stage Your Specimen 1 Your Specimen 2 All your
Specimens
Total
Prophase
Metaphase
Anaphase
Telophase
Interphase
Published values for prophase, metaphase, anaphase and telophase respectively
are 7.1, 6.5, 2.4, and 3.8 minutes.
a. What stage of the cell cycle has the longest duration? Why?
This study source was downloaded by 100000801932233 from CourseHero.com on 05-03-2022 22:41:55 GMT -05:00
https://www.coursehero.com/file/117888393/Activity-8-The-Cell-Cycle-Answer-Sheet-rev2021docx/ b. Does your class data approximate the published values for the different stages? Why or why not?
Additional Questions to Answer:
1. During what stage of the cell cycle is the nuclear material doubled?
2. Are the stages of mitosis occuring simultaneously on a meristem?
3. In what material is the coded information of the cell stored?
4. What should be the chromosome number in daughter nuclei formed by mitotic
division of cells having the following chromosome numbers? 16 ____________ 68 _____________ 22 ____________ 8 ______________
Submission Note: 1. Save the document in x.pdf format with the file name: Section_Group#_ ACTIVITY# (ex. N03_Group5_Activity08)
2. Upload the file to the corresponding module under the submission page in the CANVAS.
Table of Contributions: Indicate the work contribution of the group members below. Member with no participation will be graded zero (0).
Name Contribution Remarks
1. 2. 3. 4. 5.
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