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PCV Test Procedure and Significance

The document summarizes the packed cell volume (PCV) or hematocrit (HCT) lab procedure. It describes how whole blood is centrifuged to pack red blood cells, allowing measurement of the volume they occupy as a percentage of total blood volume. The objectives are to determine the volume of red blood cells in 100ml of blood. The procedure involves filling a microhematocrit tube with blood via capillary action, sealing it, centrifuging for 5 minutes at 12,000g, and reading the results with a microhematocrit reader. Normal ranges provided are 45-54% for adult males, 36-47% for adult females, 40-45% for children, and 54-64% for infants.

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Jalal Albadri
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485 views2 pages

PCV Test Procedure and Significance

The document summarizes the packed cell volume (PCV) or hematocrit (HCT) lab procedure. It describes how whole blood is centrifuged to pack red blood cells, allowing measurement of the volume they occupy as a percentage of total blood volume. The objectives are to determine the volume of red blood cells in 100ml of blood. The procedure involves filling a microhematocrit tube with blood via capillary action, sealing it, centrifuging for 5 minutes at 12,000g, and reading the results with a microhematocrit reader. Normal ranges provided are 45-54% for adult males, 36-47% for adult females, 40-45% for children, and 54-64% for infants.

Uploaded by

Jalal Albadri
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PDF, TXT or read online on Scribd
  • Introduction and Principles
  • Procedure and Results

Mazaya university college fifth stage

Pharmacy Department Lab Training


[Link]. Duaa Abd-Alazem. A Lab:4
----------------------------------------------------------------------------------------------------------------------
Packed Cell Volume (PCV) or
Haematocrit (HCT) Value
Introduction and principle:
Whole blood is centrifuged for maximum [Link] packing. The space occupied by
the [Link] is measured and expressed as a percentage of the whole blood volume.
Objectives:
To determine the volume or the amount of the [Link] in 100 ml (dl) of blood.

It requires less blood and also less time to determine a haematocrit.


—
— Materials And instruments
— 1-Microhaematocrit tube contain heparin and show a red ring at
the end of the tube
— 2-Microhaematocrit centrifuge
— 3. Plastic seal
— 4-Microhaematocrit reader

1
Mazaya university college fifth stage
Pharmacy Department Lab Training
[Link]. Duaa Abd-Alazem. A Lab:4
----------------------------------------------------------------------------------------------------------------------

Procedure

1-Place the capillary tube in the blood sample allowing blood to enter the tube by

capillary action. Leave the last 15mm unfilled.

2-Seal the tube with modeling clay. Make sure there is no air trapped between the

clay and the column of blood.

3-Place the tube in the microhematocrit centrifuge with the sealed end to the outer

rim and centrifuge at 12000g for 5min.

4-Read the result using a microhematocrit reading device.

Results:

Normal ranges:

Adult males = 45 - 54%.


Adult females = 36 - 47%.
Children= 40-45%
Infants= 54-64%

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