B I O L O GY

INVESTIGATORY
P R O JE C T
Topic- SICKLE CELL ANEMIA
PROJECT BY- HARIOM KUMAR ROY
CLASS- XII-B
GUIDED BY- MR.RAHUL DEV MANDAL
BOARD ROLL NO-
Biology Investigatory Project Hariom kumar roy

CERTIFICATE

This is to certify that Hariom Kumar Roy student

of class XII-B has successfully . Completed the
Biology project under the guidance of Mr. Rahul
dev mandal. This project is genuine and is not
plagiarism of any kind . The references used in
making this project file are declared at the end of
the file.

External’s Sign: Internal’s Sign:

Principal signature
School stamp

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A C K N O W L E D GE M E N T

I w i s h t o e x p r es s m y d ee p g r a t i t u d e a n d s i n c e r e
thanks to the principal ,Mr Shibu V . raju , HOLY
MARY INTERNATIONAL SCHOOL,DARBHANGA
his encouragement and for all the facilities he
has provided for this project.I extend my hearty
thanks to my class teacher Mr. Rahul Dev Mandal
who guided me to the successful completion of
this project. I take this opportunity to express
my deep sense of gratitude for their invaluable
guidance,constant encouragement and immense
motivation which has sustained my efforts at all
stages of this project work. I can’t forget to offer
my sincere thanks to my classmates who helped
me to carry out this project work successfully
and for their valuable advice and support
which I received for them time to time.

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I n de x
S.NO Contents P g. No

1 AI M 04
2 ABSTRACT 05
3 SICKLE CELL 07
M U TA TI O N
4 SICKLE CELL 08
TR A I T
5 SIGN AND 09
SYM PT O M S
R E L A TE D T O
PAIN
6 S T EP T O 11
PR E VE NT A N D
CONTROL
COMPLICATION
7 R E F ER E N C E 23

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AI M -
TO STUDY SICKLE
CELL ANEMIA

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A B S TR A C T
Sickle cell anemia is the most common form of
sickle cell disease (SCD). SCD is a serious disorder
in which the body makes sickle-shaped red blood
cells. “Sickle-shaped” means that the red blood
cells are shaped like a crescent.
Normal red blood cells are disc-shaped and look
like doughnuts without holes in the center. They
move easily through your blood vessels. Red
blood cells contain an iron-rich protein called
hemoglobin. This protein carries oxygen from the
lungs to the rest of the body.Sickle cells contain
abnormal hemoglobin called sickle hemoglobin or
hemoglobin S. Sickle hemoglobin causes the cells
to develop a sickle, or crescent, shape.
Sickle cells are stiff and sticky. They tend to block
blood flow in the blood vessels of the limbs and
organs. Blocked blood flow can cause pain and
organ damage. It can also raise the risk for
infection.

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Normal Red Blood Cells and

Sickle cells-

Figure A shows normal red blood cells flowing freely in a

blood vessel. The inset image shows a cross-section of a
normal red blood cell with normal hemoglobin. Figure B
shows abnormal, sickled red blood cells blocking blood flow
in a blood vessel. The inset image shows a cross-section of a
sickle cell with abnormal (sickle) hemoglobin forming
abnormal strands.

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Sickle Cell Mutation

Sickle cell anemia has no widely available cure. However,

treatments to improve the anemia and lower complications
can help with the symptoms and complications of the disease
in both children and adults. Blood and marrow stem cell
transplants may offer a cure for a small number of people.
Over the past 100 years, doctors have learned a great deal
about sickle cell anemia. They know its causes, how it affects
the body, and how to treat many of its complications.
Sickle cell anemia varies from person to person. Some people
who have the disease have chronic (long-term) pain or
fatigue (tiredness). However, with proper care and

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treatment, many people who have the disease can have

improved quality of life and reasonable health much of the
time.
Because of improved treatments and care, people who have
sickle cell anemia are now living into their forties or fifties, or
longer.

 What Causes Sickle Cell

Anemia?
Sickle cell anemia is an inherited disease. People who have
the disease inherit two genes for sickle hemoglobin—one
from each parent.
Sickle hemoglobin causes red blood cells to develop a sickle,
or crescent, shape. Sickle cells are stiff and sticky. They tend
to block blood flow in the blood vessels of the limbs and
organs. Blocked blood flow can cause pain and organ
damage. It can also raise the risk for infection.

Sickle Cell Trait

People who inherit a sickle hemoglobin gene from one
parent and a normal gene from the other parent have sickle
cell trait. Their bodies make both sickle hemoglobin and
normal hemoglobin.

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People who have sickle cell trait usually have few, if any,
symptoms and lead normal lives. However, some people may
have medical complications.
People who have sickle cell trait can pass the sickle
hemoglobin gene to their children. The following image
shows an example of an inheritance pattern for sickle cell
trait.
Example of an Inheritance Pattern for Sickle Cell Trait
When both parents have a normal gene and an abnormal
gene, each child has a 25 percent chance of inheriting two
normal genes; a 50 percent chance of inheriting one normal
gene and one abnormal

 What Are the Signs and

Symptoms of Sickle Cell Anemia
The signs and symptoms of sickle cell anemia vary. Some
people have mild symptoms. Others have very severe
symptoms and often are hospitalized for treatment.Sickle cell
anemia is present at birth, but many infants don't show any
signs until after 4 months of age.The most common signs and
symptoms are linked to anemia and pain. Other signs and
symptoms are linked to the disease's complications.

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Signs and Symptoms Related to

Anemia
The most common symptom of anemia is fatigue (feeling
tired or weak). Other signs and symptoms of anemia include:
• Shortness of breath
• Dizziness
• Headaches
• Coldness in the hands and feet
• Paler than normal skin or mucous membranes (the tissue
that lines your nose, mouth, and other organs and body
cavities)
• Jaundice (a yellowish color of the skin or whites of the eyes)

Signs and Symptoms Related to Pain

Sudden pain throughout the body is a common symptom of
sickle cell anemia. This pain is called a sickle cell crisis. Sickle
cell crises often affect the bones, lungs, abdomen, and joints.
These crises occur when sickled red blood cells block blood
flow to the limbs and organs. This can cause pain and organ
damage.The pain from sickle cell anemia can be acute or
chronic, but acute pain is more common. Acute pain is
sudden and can range from mild to very severe. The pain

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usually lasts from hours to as long as a week or more.Many

people who have sickle cell anemia also have chronic pain,
especially in their bones. Chronic pain often lasts for weeks
or months and can be hard to bear and mentally draining.
Chronic pain may limit your daily activities.
Almost all people who have sickle cell anemia have painful
crises at some point in their lives. Some have these crises less
than once a year. Others may have crises once a month or
more. Repeated crises can damage the bones, kidneys, lungs,
eyes, heart, and liver. This type of damage happens more
often in adults than in children.Many factors can play a role
in sickle cell crises. Often, more than one factor is involved
and the exact cause isn't known.

You can control some factors. For example, the risk

of a sickle cell crisis increases if you're dehydrated (your
body doesn't have enough fluids). Drinking plenty of
fluids can lower the risk of a painful crisis.You can't
control other factors, such as infections.Painful crises
are the leading cause of emergency room visits and
hospital stays for people who have sickle cell anemia.

Complications of Sickle Cell

Anemia
Sickle cell crises can affect many parts of the body and cause
many complications.

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 Hand-Foot Syndrome
Sickle cells can block the small blood vessels in the hands and
feet in children (usually those younger than 4 years of age).
This condition is called hand-foot syndrome. It can lead to
pain, swelling, and fever.Swelling often occurs on the back of
the hands and feet and moves into the fingers and toes. One
or both hands and/or feet might be affected at the same
time.

 Splenic Crisis
The spleen is an organ in the abdomen. Normally, it filters
out abnormal red blood cells and helps fight infections.
Sometimes the spleen may trap red blood cells that should
be in the bloodstream. This causes the spleen to grow large
and leads to anemia.If the spleen traps too many red blood
cells, you may need blood transfusionsuntil your body can
make more cells and recover.

 Infections
Both children and adults who have sickle cell anemia may get
infections easily and have a hard time fighting them. This is
because sickle cell anemia can damage the spleen, an organ
that helps fight infections.
Infants and young children who have damaged spleens are
more likely to get serious infections that can kill them within

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hours or days. Bloodstream infections are the most common

cause of death in young children who have sickle cell
anemia.Medicines and vaccines can help prevent severe
illness and death. For example, vaccines are available for
infections such as meningitis, influenza, and hepatitis.
Getting treatment right away for high fevers (which can be a
sign of a severe infection) also helps prevent death in infants
and children who have sickle cell anemia.It's also important
to get treatment right away for a cough, problems breathing,
bone pain, and headaches.

 Acute Chest Syndrome

Acute chest syndrome is a life-threatening condition linked to
sickle cell anemia. This syndrome is similar to pneumonia. An
infection or sickle cells trapped in the lungs can cause acute
chest syndrome.People who have this condition often have
chest pain, shortness of breath, and fever. They also often
have low oxygen levels and abnormal chest X ray results.

 Pulmonary Hypertension
Damage to the small blood vessels in the lungs makes it hard
for the heart to pump blood through the lungs. This causes
blood pressure in the lungs to rise.Increased blood pressure
in the lungs is called pulmonary hypertension (PH). Shortness
of breath and fatigue are the main symptoms of PH.

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 Delayed Growth and Puberty in

Children
Children who have sickle cell anemia often grow more slowly
than other children. They may reach puberty later. A
shortage of red blood cells causes the slow growth rate.
Adults who have sickle cell anemia often are slender or
smaller in size than other adults.

 Stroke
Two forms of stroke can occur in people who have sickle cell
anemia. One form occurs if a blood vessel in the brain is
damaged and blocked. This type of stroke occurs more often
in children than adults. The other form of stroke occurs if a
blood vessel in the brain bursts.Either type of stroke can
cause learning problems and lasting brain damage, long-term
disability, paralysis (an inability to move), or death.

 Eye Problems
Sickle cells also can affect the small blood vessels that deliver
oxygen-rich blood to the eyes. Sickle cells can block these
vessels or cause them to break open and bleed. This can
damage the retinas—thin layers of tissue at the back of the
eyes. The retinas take the images you see and send them to
your brain.This damage can cause serious problems,
including blindness.
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 Priapism
Males who have sickle cell anemia may have painful,
unwanted erections. This condition is called priapism. It
happens because the sickle cells block blood flow out of an
erect penis. Over time, priapism can damage the penis and
lead to impotence.

 Gallstones
When red blood cells die, they release their hemoglobin. The
body breaks down this protein into a compound called
bilirubin. Too much bilirubin in the body can cause stones to
form in the gallbladder, called gallstones.Gallstones may
cause steady pain that lasts for 30 minutes or more in the
upper right side of the belly, under the right shoulder, or
between the shoulder blades. The pain may happen after
eating fatty meals.
People who have gallstones may have nausea (feeling sick to
the stomach), vomiting, fever, sweating, chills, clay-colored
stools, or jaundice.

 Ulcers on the Legs

Sickle cell ulcers (sores) usually begin as small, raised, crusted
sores on the lower third of the leg. Leg sores may occur more
often in males than in females. These sores usually develop in
people who are aged 10 years or older.The cause of sickle cell

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ulcers isn't clear. The number of ulcers can vary from one to
many. Some heal quickly, but others persist for years or
come back after healing.

 Multiple Organ Failure

Multiple organ failure is rare, but serious. It happens if you
have a sickle cell crisis that causes two out of three major
organs (lungs, liver, or kidneys) to fail. Often, multiple organ
failure occurs during an unusually severe pain
crisis.Symptoms of this complication are fever, rapid
heartbeat, problems breathing, and changes in mental status
(such as sudden tiredness or confusion).

How is Sickle Cell Anemia

Diagnosed?
A simple blood test, done at any time during a person's
lifespan, can detect whether he or she has sickle hemoglobin.
However, early diagnosis is very important.
In the United States, all States mandate testing for sickle cell
anemia as part of their newborn screening programs. The
test uses blood from the same blood samples used for other
routine newborn screening tests. The test can show whether
a newborn infant has sickle hemoglobin.Test results are sent
to the doctor who ordered the test and to the baby's primary
care doctor. It's important to give the correct contact

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information to the hospital. This allows the baby's doctor to

get the test results as quickly as possible.
Health providers from a newborn screening followup
program may contact you directly to make sure you're aware
of the test results.If the test shows some sickle hemoglobin, a
second blood test is done to confirm the diagnosis. The
second test should be done as soon as possible and within
the first few months of life.
The primary care doctor may send you to a hematologist for
a second blood test. A hematologist is a doctor who
specializes in blood diseases and disorders. This doctor also
can provide treatment for sickle cell disease if needed.
Doctors also can diagnose sickle cell disease before birth. This
is done using a sample of amniotic fluid or tissue taken from
the placenta. (Amniotic fluid is the fluid in the sac
surrounding a growing embryo. The placenta is the organ
that attaches the umbilical cord to the mother's
womb.)Testing before birth can be done as early as 10 weeks
into the pregnancy. This testing looks for the sickle
hemoglobin gene, rather than the abnormal hemoglobin that
the gene makes.

 New Treatments
Research on blood and marrow stem cell transplants, gene
therapy, and new medicines for sickle cell anemia is ongoing.
The hope is that these studies will provide better treatments
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for the disease. Researchers also are looking for a way to

predict the severity of the disease.

 Blood and Marrow Stem Cell

Transplant
A blood and marrow stem cell transplant can work well for
treating sickle cell anemia. This treatment may even offer a
cure for a small number of people.The stem cells used for a
transplant must come from a closely matched donor. The
donor usually is a close family member who doesn't have
sickle cell anemia. This limits the number of people who may
have a donor.
The transplant process is risky and can lead to serious side
effects or even death. However, new transplant approaches
may improve treatment for people who have sickle cell
anemia and involve less risk.Blood and marrow stem cell
transplants usually are used for young patients who have
severe sickle cell anemia. However, the decision to give this
treatment is made on a case-by-case basis.
Researchers continue to look for sources of bone marrow
stem cells—for example, blood from babies' umbilical cords.
They also continue to look for ways to reduce the risks of this
procedure.

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 Gene Therapy
Gene therapy is being studied as a possible treatment for
sickle cell anemia. Researchers want to know whether a
normal gene can be put into the bone marrow stem cells of a
person who has sickle cell anemia. This would cause the body
to make normal red blood cells.
Researchers also are studying whether they can "turn off"
the sickle hemoglobin gene or "turn on" a gene that makes
red blood cells behave normally.

 New Medicines
Researchers are studying several medicines for sickle cell
anemia. They include:
• Decitabine: Like hydroxyurea, this medicine prompts the
body to make fetal hemoglobin. Fetal hemoglobin helps
prevent red blood cells from sickling and improves anemia.
Decitabine might be used instead of hydroxyurea or added to
hydroxyurea.
• Adenosine A2a receptor agonists: These medicines may
reduce pain-related complications in people who have sickle
cell anemia.
• 5-HMF: This natural compound binds to red blood cells and
increases their oxygen. This helps prevent the red blood cells
from sickling.

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Take Steps To Prevent and Control Complications

Along with adopting healthy lifestyle habits, you can take
other steps to prevent and control painful sickle cell crises.
Many factors can cause sickle cell crises. Knowing how to
avoid or control these factors can help you manage your
pain.You may want to avoid decongestants, such as
pseudoephedrine. These medicines can tighten blood vessels,
making it harder for red blood cells to move smoothly
through the vessels.
Avoid extremes of heat and cold. Wear warm clothes outside
in cold weather and inside of air-conditioned rooms. Don't
swim in cold water. Also, be cautious at high altitudes; you
may need extra oxygen.If possible, avoid jobs that require a
lot of heavy physical labor, expose you to extremes of heat or
cold, or involve long work hours.
Don't travel in airplanes in which the cabins aren't
pressurized (that is, no extra oxygen is pumped into the
cabin). If you must travel in such an airplane, talk with your
doctor about how to protect yourself.

 Ongoing Care
Get a flu shot and other vaccines to prevent infections. You
also should see your dentist regularly to prevent infections
and loss of teeth. Contact your doctor right away if you have
any signs of an infection, such as a fever or trouble
breathing.For people who have sickle cell anemia, just like for
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everyone else, regular medical care and treatment for health

issues are important. Your checkups may include extra tests
for possible kidney, lung, and liver diseases. See a sickle cell
anemia expert regularly. Also, see an eye doctor regularly to
check for damage to your eyes.
Learn the signs and symptoms of a stroke. They include:
• Sudden weakness
• Paralysis (an inability to move) or numbness of the face,
arms, or legs, especially on one side of the body
• Confusion
• Trouble speaking or understanding speech
• Trouble seeing in one or both eyes
• Problems breathing
• Dizziness, trouble walking, loss of balance or coordination,
and unexplained falls
• Loss of consciousness
• Sudden and severe headache
If you think you’re having a stroke, call 9–1–1 right away. Do
not drive to the hospital or let someone else drive you. Call
an ambulance so that medical personnel can begin life-saving
treatment on the way to the emergency room.
Get treatment and control any other medical conditions you
have, such as diabetes.Talk with your doctor if you're
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pregnant or planning to become pregnant. Sickle cell anemia

can worsen during pregnancy. You'll need special prenatal
care.
Women who have sickle cell anemia also are at increased risk
for an early birth or a low-birth-weight baby. However, with
early prenatal care and frequent checkups, you can have a
healthy pregnancy.

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 Reference
• Biology textbook, NCERT
• www.wikipedia.com
• www.google/images.com
• www.NHLBI (National Heart, Lung, and Blood
Institute).com

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