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Spotters

This document lists various spotters used in clinical laboratories including reagents, equipment, and tests. It discusses Selivanoff's reagent for detecting fructose, osazone formation for detecting glucose, a GTT test to analyze glucose tolerance, sulfur powder for detecting bile salts, Benedict's reagent for detecting reducing sugars, an albuminometer for detecting protein in urine, a glucometer that uses amperometric technology to measure blood glucose levels from a small blood sample, urine strips that can read various parameters from a urine sample, and an ABG analyzer that uses potentiometry and ion selective electrodes to measure acids, bases, and electrolytes in blood.

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Chemmu Karama
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277 views3 pages

Spotters

This document lists various spotters used in clinical laboratories including reagents, equipment, and tests. It discusses Selivanoff's reagent for detecting fructose, osazone formation for detecting glucose, a GTT test to analyze glucose tolerance, sulfur powder for detecting bile salts, Benedict's reagent for detecting reducing sugars, an albuminometer for detecting protein in urine, a glucometer that uses amperometric technology to measure blood glucose levels from a small blood sample, urine strips that can read various parameters from a urine sample, and an ABG analyzer that uses potentiometry and ion selective electrodes to measure acids, bases, and electrolytes in blood.

Uploaded by

Chemmu Karama
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
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Download as DOCX, PDF, TXT or read online on Scribd
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SPOTTERS

1.SELIWANOFF’S REAGENT

A. 0.05g resorcinol , 100ml dilute HCL

B. Detection of fructose containing carbohydrate,

2. A. Osazoneformation –Yellow needle shaped crystal (Glucosazones)

B. Phenyl Hydrazine hydrochloride

3. A. Urinometer

B. Urine Specific gravity measurement.

4. GTT TEST ANALYSIS

GTT shows normal , 1 hr value will peak and after 2 hr value comes normal.

5. SULPHUR POWDER

A. Hay’s test

B. Test for Bile salt

6. A. Hedge- Hog shaped crystals

B. Phenyl Hydrazine hydrochloride

7. A. ketone bodies and urine Glucose

B. Uncontrolled Diabetes and prolonged starvation

8. A. Arginine and Phenylalanine

B. RF value = Distance by the compounds ( Solute front)

Distance moved by solvent front

9. BENEDICT’S REAGENT

A. Sodium citrate 173gs ,

Sodium carbonate 100g

Copper sulphate 17.3 g , Distilled water

B. Detection Reducing Sugars


10. A. Sun flower shaped crystals

B. Phenyl Hydrazine hydrochloride

11. A. Albuminometer

B. Determining the presence and amount of protein albumin in a urine sample

12.A. Urine Albumin (protein ), Blood in urine

B. Albuminuria in Kidney diseases, Hematuria bleeding in the urinary tract.

13. A. Alanine and Valine

B. RF value = Distance by the compounds ( Solute front)

Distance moved by solvent front

14. A. Volumetric Flask ( Standard flask )

B. These are mainly used to make up final volume of the reagent accurately.

15. A. Abnormal ( Multiple Myeloma )

B. M- Band

16. A. pH Meter

B. Used to measure pH of buffers, reagents, body fluids.

17. A. Spectrophotometer.

B. Used for quantitative estimation of various biological fluids.

Used in Enzyme assay and kinetic studies

18. A. Cobas H 232 ( Measure the cardiac marker )

B. Detection of cardiac marker like D-dimer, cardiac troponin T, BNP test, Myoglobulin
and CK-MB.

19. A. Desicator

B. uses for keep the hygroscopic chemicals like trichloroacetic acid, ferric chloride.
20. A. Glucometer

B. PRINCIPLE : ( Amperometric technology )When blood sample is applied the strip electrons
are formed by the reaction between glucose oxidase & blood glucose .The electrical current is
measured by the meter and correlates with concentration of glucose in the blood sample.

21.A. Urine strip

B. uses : read the urine ph, specific gravity, glucose,protein etc…

22. A. ABG analyser

B. Potentiometry, ISE (ion selective electrodes)

23. A. Follin Wu tube

B.Determination of blood sugar by follin wu method.

24. A. Colorimeter.

B. cuvette, Monochromatic light, slit,detector,galvanometer.

25. A. Flat bottomed flask

B. Mainly used for heating liquids.

26.A. Measuring cylinder

B. used to Measure Specific volume

27. A. Automated Pipettes

B. Used to Measure and deliver accurate Volume.

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