This document outlines a science experiment to observe and cultivate protozoa through microscopy. Students are instructed to create two cultures - one using vase water and another using macerated lettuce leaves. They observe the cultures under a microscope after 5 days and identify the protozoa present. The results charts show which organisms were found in each sample. In conclusion, the students determine which culture media allowed for proper protozoa growth.
This document outlines a science experiment to observe and cultivate protozoa through microscopy. Students are instructed to create two cultures - one using vase water and another using macerated lettuce leaves. They observe the cultures under a microscope after 5 days and identify the protozoa present. The results charts show which organisms were found in each sample. In conclusion, the students determine which culture media allowed for proper protozoa growth.
This document outlines a science experiment to observe and cultivate protozoa through microscopy. Students are instructed to create two cultures - one using vase water and another using macerated lettuce leaves. They observe the cultures under a microscope after 5 days and identify the protozoa present. The results charts show which organisms were found in each sample. In conclusion, the students determine which culture media allowed for proper protozoa growth.
This document outlines a science experiment to observe and cultivate protozoa through microscopy. Students are instructed to create two cultures - one using vase water and another using macerated lettuce leaves. They observe the cultures under a microscope after 5 days and identify the protozoa present. The results charts show which organisms were found in each sample. In conclusion, the students determine which culture media allowed for proper protozoa growth.
ST. VIATOR BILINGUAL INTERNATIONAL SCHOOL VERSION 02
ASSESSMENT DATE 13 - 02 - 18 SUBJECT: Science UNIT TITLE: The earth, our home
TOPIC: Protozoa TERM:
NAME: CLASS: SCORE:
OBJETIVE:Observe various free-living ciliated and flagellated unicellular microorganisms
PREPARED BY: Betty Carolina López S. CHECKED BY: Mauricio Aponte
CULTIVATION AND OBSERVATION OF PROTOZOA
THROUGH THE MICROSCOPE
INTRODUCTION
Microscopic life can be found in
ponds, lakes, streams, rivers, ocean backwater estuaries, and, surprisingly, rain puddles that have been in existence for a few days. Water samples can easily be collected using wide-mouth glass jars with tight-fitting lids. The collection jars you use should be completely clean and detergent free. A great place to find protozoans and tiny water animals is in a fresh water fish tank or in vase water (Taken from https://microscope-microscope.org/microscope-applications/culturing-protozoa/)
PROBLEM As you know, environmental conditions are not always stable; some resources may be scarce or limited for living things in this case the protozoa, so your task will be to determine: which of the culture media allow the proper growth of protozoa?
MATERIALS
Lab's material Optical microscope. Slide / Coverslips Lab coat masks Rice paper Small towel Dropper Biological material 2 Glass bottles to Cultivate the protozoa Lettuce leaves Vase water (minimum 5 days) Cling wrap or Gauze and rubber bands Rain water or bottle water
VASE WATER: From July 19 th to July 23 th BOTTLE WATER: From July 21 rst to July 23 th CULTURE OF PROTOZOA: From July 23 th to July 27 th PROCEDURE I: CULTURE OF PROTOZOA (Second part of the album; do not forget to take pictures of your activity).
FIRST CULTIVATION:
Get vase water; if you do not have it, you should to place flowers in a vase for 5 days. Then, take one of the glass bottles and add bottled water or rainwater to the middle of the glass bottle, let it stand for 3 days. After three days, add the vase water that you have to fill the glass bottle. Cover the bottle with the gauze or the cling wrap and secure it with the rubber band. Leave the jar in a cool place for 5 days.
SECOND CULTIVATION Take the other glass bottle and add bottled water or rainwater to the middle of the glass bottle, let it stand for 3 days. After three days, place 1 or 2 leaves of MACERATED lettuce in the bottom of the jar. Cover the bottle with the gauze or the cling wrap and secure it with the rubber band. Leave the jar in a cool place for 5 days.
PROCEDURE II: OBSERVATION OF PROTOZOA IN THE
MICROSCOPE
Carry the microscope with both hands to the microscope storage area and properly transport one microscope to your working area.
Take a slide and place samples of the water in the glass with the dropper. Take the samples near the surface of the water and in the middle area of the bottle, always in the vicinity of the plant remains. Cover it with a cover slip.
Dry it below with the small towel.
Observe the microscope, first from the smallest 4x magnification, and then up to 40x. PROCEDURE III: In these drawings, different types of protozoa are represented. Use them to identify the protozoa present in the samples.
RESULTS AND ANALYSIS:
A. Complete the followings charts taking into account the observations of the cultures.
TYPE OF SAMPLE 1 SAMPLE 2 SAMPLE 3 ORGANISM
CULTURE TYPE OF SAMPLE 1 SAMPLE 2 SAMPLE 3 ORGANISM CULTURE
B. Protozoa are eukaryotic unicellular beings of heterotrophic nutrition, which
need to live in a humid environment. What does it mean that protozoa are eukaryotic heterotroph beings? ……………………………………………………………………………………………… ……………………………………………………………………………………………… ……………………………………………………………………………………………… ……………………………………………………………………………………………… ……………………………………………………………………………………………… ………………………………………………………………………………………………
C. Taking into account the information that you have collect it in the charts, What can you identify in the results? ……………………………………………………………………………………… ……………………………………………………………………………………… ……………………………………………………………………………………… ……………………………………………………………………………………… ……………………………………………………………………………………… ………………………………………………………………………………………
