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Function of Microbiology Lab Instruments

This document lists and describes 20 pieces of common equipment used in a microbiology laboratory, including their functions. Some key equipment discussed are agar plates containing different types of agar like nutrient agar, MacConkey agar, and blood agar which are used to culture and isolate different microorganisms. Other important equipment mentioned are incubators to culture microbes at suitable temperatures, autoclaves to sterilize materials, biological safety cabinets to safely work with pathogens, and petri dishes, inoculating loops, and needles to transfer microbes.

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Kavilashini Subramaniam
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165 views2 pages

Function of Microbiology Lab Instruments

This document lists and describes 20 pieces of common equipment used in a microbiology laboratory, including their functions. Some key equipment discussed are agar plates containing different types of agar like nutrient agar, MacConkey agar, and blood agar which are used to culture and isolate different microorganisms. Other important equipment mentioned are incubators to culture microbes at suitable temperatures, autoclaves to sterilize materials, biological safety cabinets to safely work with pathogens, and petri dishes, inoculating loops, and needles to transfer microbes.

Uploaded by

Kavilashini Subramaniam
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as DOCX, PDF, TXT or read online on Scribd
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Discussion

No. Equipment Functions

A petri dish containing agar in combination with a


1. Agar Plate growth medium to culture microorganisms such as
bacteria

Nutrient Agar To produce the bacterial lawns needed for antibiotic


2.
sensitivity tests
3. MacConkey Agar To isolate the gram-negative enteric bacteria

Blood Agar To grow fastidious organisms and to differentiate


4.
bacteria based on their hemolytic properties
5. Slant Agar Is used to culture bacterial cells for identification
6. Petri Plate To grow culture of different organisms

Wire Inoculating Loop Used in the cultivation of microbes on plates by


7.
transferring inoculum for streaking
8. Inoculating Needle Used to transfer and inoculate living microorganisms
9. Sterile Cotton Swab It is used for microbiological sampling
An enclosed ventilated laboratory workspace for
10. Biological Safety Cabinet safely working with materials that are potentially
contaminated with pathogens
Enclosed worktop to keep it entirely sterile, keeping
11. Laminar Air Flow the test subject safe from contaminants and keep the
scientist safe from any possible threats

Autoclave To sterilization of glassware from microbes and to


12.
prepare media and saline solutions
To culture microbes, bacteria, fungi at a suitable
13. Incubator temperature which is maintain and constant in the
incubator
It divides the plate into several squares division and
14. Quebec Colony Counter the colonies are magnified which makes it easier to
count the number of colonies that have developed

Analytical Balance It is used to weigh small quantities of chemicals and


15.
samples precisely
16. Waterbath To maintain temperature without a gradient of heat
17. Lab Oven A dry heat sterilization
18. Microbial Powder Powder to make agar plates
19. Water Distillation Unit To filter tap water from most impurities/bacteria
Also known as ab environmental rooms, are
20. Cold Room designed to control temperature and humidity. 1.6◦C
-48.8◦C
References

1. Arya, N. (2018). The Numerous Uses and Methods of Using a Petri Dish. Retrieved on 22
February 2019 from www.sciencestruck.com

2. Aryal, S. (2015). Blood Agar-Composition, Preparation, Uses and Pictures. Retrieved on 22


February 2019 from www.microbiology.com

3. Aryal, S. (2015). MacConkey Agar-Composition, Principle, Uses, Preparation and Colony


Morphology. Retrieved on 22 February 2019 from www.microbiology.com

4. Aryal, S. (2015). Nutrient Agar: Composition, Preparation and Uses. Retrieved on 22


February 2019 from www.microbiology.com

5. Dr.T.V.Rao (2018). Optimal Use of Inoculating Loop in Microbiology? Retrieved on 22


February 2019 from www.linkedin.com

6. Lehman, C. (2018). What Are Agar Slants? Retrieved on 22 February 2019 from
www.sciencing.com

7. Mohammed Hamad, M. N. (2018). microbiology lab instruments. Retrieved on 24 February


2018 from https://www.researchgate.net

8. Quinlan, S. (2018). Types of Agar Plates. Retrieved on 22 February 2019 from


www.sciencing.com

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