Sorbtech Seperation Guide

SORBTECH
S O R B E N T T E C H N O L O G I E S
Chromatography and Laboratory Products
2012
Bulk Sorbents Polymeric Resins TLC Plates
SPE Cartridges GC Columns Syringe Filters
Flash Cartridges & Systems FPLC Columns HPLC & UHPLC
Laboratory Equipment Solvent Evaporation Plasticware
Ordering - Sales, Terms, & Conditions

Phone: Fax: Email: Online: 866-SORBTEC (767-2832) 770-936-0326 sales@sorbtech.com www.sorbtech.com Mail: Sorbent Technologies, Inc. 5955 Peachtree Corners East Suite A Norcross, GA 30071
Payment Terms:
-Payment by valid credit card unless otherwise stated, FOB, Norcross, Georgia. -Net 30 days is subject to approval and agreement. -Shipping and Insurance are prepaid and added to invoice. -A charge of 1.5% interest per month late charge will be applied to accounts after net 30. -Orders (domestic and international) may be payable by wired funds.
Pricing and Discounts:

-To better serve our customers each order is custom quoted. -Quantity and blanket order discounts are available.
Purchase Orders:
-Please call us at 866-SORBTEC (767-2832) or Intl 770-936-0323 to be approved for an online PO. Once your name and company is approved, you may do future online POs under an online username that you created upon registration on www.sorbtech.com.
Credit Cards:
-We accept Mastercard, Visa, & American Express -Include your credit card number, expiration date, and CV code if ordering by credit card.
International Orders:
-Please call 011-770-963-0323 to place your international order. -Because shipping and insurance are prepaid and added to the invoice, we do not accept international orders online. -All international orders must be prepaid. -Orders under $400 may be paid by credit card (Visa, Mastercard or American Express) or wired funds. -Orders over $400 are payable by wired funds only.
Return Policy:
-Please contact us immediately if an item is to be returned. -We will issue a R.A.N. (Return Authorization Number) when and where appropriate. -Returns are subject to approval and may incur a 25% restocking fee. -If a product was shipped in error, no restocking fee will be incurred.
Damaged Goods:
-Please inspect your shipment upon receipt. If external damage, accept only after driver has noted damage on all copies of delivery receipt. -If you receive Damaged Goods or a shortage, contact the carrier immediately to make a claim. Save all packing materials to be inspected by carrier. -All claims must be made within 5 days of receipt of shipment.
Warranty
-Sorbent Technologies, Inc. warrants its products to be free from known defects and shall conform to the description and specifications of such products as specified on the label, and in the catalog, data sheets, or literature at the time of the sale. Specifications are subject to change without notice. -Sorbent Technologies, Inc.s warranty is strictly limited to the value of the product sold. -This warranty is exclusive, and Sorbent Technologies, Inc. makes no other warranty, express or implied, including without limitation any implied warranties of merchantability and/or fitness for any particular purpose. -Sorbent Technologies, Inc. is not responsible for any direct, indirect, special, incidental, compensatory, or consequential damages. -If you are not fully satisfied with any Sorbent Technologies, Inc.s products, contact us within 30 days of purchase and we will (at our discretion) provide replacement or credit. All trademarks are properties of their respective owners. Copyright 2012 Sorbent Technologies, Inc. All rights reserved.
SORBTECH
Providing Purity and Performance, Service and Selection

Chromatography
Page
Laboratory Equipment

Sorbtech Chromatography ....................................................................................... 4 Silica Gel ........................................................................................................................................ 5

Granular ........................................................................................................................................6 Silica Gel for ASTM D1319-01 & ISO 3837 ................................................................8 Spherical ......................................................................................................................................9 Bonded Phases.......................................................................................................................12 Alumina . .....................................................................................................................................14 Alumina Solvent Purification ........................................................................................18 Specialty Adsorbents ...................................................................................................19 Dry Column Chromatography ...........................................................................20 Polymeric Resins . ..............................................................................................................22 SPE Cartridges .....................................................................................................................30 Chromabond ..........................................................................................................................32 Jordi .............................................................................................................................................40 TLC Plates . ...............................................................................................................................42 TLC Accessories .....................................................................................................................50 Flash Cartridges .................................................................................................................51 Sorbtech Flash Cartridges ................................................................... 51 Agilent Technologies..........................................................................................................54 EZ Flash System...................................................................................................................56 FPLC Columns........................................................................................................................60 Econoline and Econoline LP......................................................................................60 Upscale & IsoKrom. .............................................................................. 61 Syringe Filters........................................................................................................................62 HPLC Columns.......................................................................................................................63 Sorbtech.....................................................................................................................................63 Grace Vydac..............................................................................................................................64 Macherey-Nagel. ....................................................................................................................66 MCI Gel........................................................................................................................................73 Jordi...............................................................................................................................................76 HPLC Column Packing System.............................................................................78 Prep Packing System...........................................................................................................79 HPLC Systems. .......................................................................................................................80 GC Columns.............................................................................................................................84
Page Laboratory Equipment................................................................ 94

Centrifan PE - Personal Evaporator. ............................................................................94 Centrifan RE - Recirculating Evaporator..................................................................96 Mini and Micro Centrifuges............................................................................................98 Vortex Mixer. .............................................................................................................................99 Microplate Mixer....................................................................................................................99 Homogenizer...........................................................................................................................99 Variable Speed Rocker.................................................................................................... 100 Orbital & Linear Shaker................................................................................................... 100 Hotplate Stirrers.................................................................................................................. 100 Weigh Dishes/ Cuvettes / Petri Dishes. ................................................................. 102 PCR Products......................................................................................................................... 103 Pipet Tips................................................................................................................................. 104 Pipettors ................................................................................................................................. 105 Serilogical Pipets ............................................................................................................... 105 Tubes. ......................................................................................................................................... 106 Gloves and Racks................................................................................................................ 107 Racks/ Boxes/ Burette and Dispensers ............................................................... 108 Microscopes.......................................................................................................................... 109
Visit our website for our complete product selection and to search our application database.
WWW.SORBTECH.COM
Sorbent Technologies, Inc. Toll Free: 866.SORBTEC Fax: 770.936.0326 www.sorbtech.com 3
Sorbtech Chromatography
Let Sorbent Technologies assist you with method development and optimization from analytical methods to full scale process. Our complete selection of standardized sorbents. and chromatographic products is designed to meet all of your needs at any budget. We supply laboratory, pilot, and industrial processes, making us the top choice for your ever-developing chromatography needs. Our exact focus on the chromatography industry translates to unparalleled selection and service for you and your facility. Sorbent Technologies, is able to provide you with competitive pricing, unmatched quality, and knowledgeable staff to help your operation succeed. In fact, well match you with a Product Specialist to personally assist you from day one. Your Product Specialist is only a phone call away. Our amazing applications database is always available on our website to describe chromatography methods for TLC, HPLC, GC, and SPE and includes both stationary and mobile phases for your target compound to assist the chemist in method development.
Dedicated to Chromatography
Since 2000 Sorbtech has been providing the highest quality adsorbents for chromatography and purification from laboratory to pilot to full-scale production. We understand the importance of your work and the necessity for consistent, high quality products. This is what separates Sorbtech from other chromatography suppliers.
Highest Quality
We pride ourselves in offering products manufactured to the highest standards. The following adsorbent characteristics contribute to higher performing products in TLC, LC, and HPLC and concurrently give you the confidence you require to not only save time but also succeed at your work. High purity adsorbents Standardized activities Narrow particle and pore distributions Consistent particle shape and surface symmetry Excellent mechanical and structural properties for pressure stability Exceptional bonding techniques
Broadest Selection
Sorbent Technologies offers a full range of standardized granular silica gels in: premium, and standard, grades. Our complete selection of silica gel products designed to perform simple to complex analytical and preparative chromatographic techniques for laboratory, pilot, and industrial process applications. Granular and Spherical Particle sizes for analytical to preparative to process applications from 2.0m to 3mm Pore sizes for small to large molecule separations (proteins & peptides) from 60 to 1000 C18, C8, C4, C2, Amino, Cyano, Phenyl, Diol, and Custom Bonded Phases Modified aluminas with three pH levels and specific Brockmann activities Ion exchange resins with superior physical and chemical properties With all of these adsorbents available at Sorbtech, finding appropriate adsorbents is easy and convenient. Allow a Sorbtech product specialist to help you develop or optimize your application or process.
Sorbent Technologies, Inc.
Toll Free: 866.SORBTEC
Fax: 770.936.0326
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Selection Guide For Normal Phase & Reverse Phase Silica Gel
Silica is our specialty. We offer the largest selection of silica gels with pore diameters ranging from 60 to 1000, particle sizes from 2m to 500m, and bonded phases from C18 to SAX (HPLC, MPLC, Flash & Gravity Grades). Sorbent Technologies offers the best selection of granular silica gels for column chromatography: TLC, MPLC, Flash, and Gravity grades. Use the Silica Guide below to help you find the right Silica for your separations.
Compound to be Purified
Functionality
Capacity
Selectivity
Chemistry Solubility
Molecular Weight
Separation Difficulty
Non Aqueous
Aqueous
MW<1,000 D
MW<10,000 D
MW<50,000 D
Non to Low Polarity
Low to Medium Polarity
Medium to High Polarity
Organic Molecules
Peptides
Polypeptides bio-polymers
3 & 5 m 10 m 15 m
20 m 40 m 20 - 45 m
40 - 75 m
75 - 200 m
Silica
C4 & C8
C18
60 70
100 200 300
500 800 1000
Silica Gel
Granular Silica Gel - MPLC, Flash, & Gravity LC Phases
Premium Rf silica gel is the finest granular silica gel on the market. This high purity product provides narrow particle and pore distribution and is designed to increase yield, making it ideal for every-day chromatography and complicated separations. With Premium Rf your lab can enjoy bulk quantities for large-scale applications, no scale-up limitation, and secured lots offered for process use.
Advantages of Premium Rf Flash Silica Gel

1. Narrow particle distribution: 40-75m +/- 5% versus leading brands +/- 10-12%.
Better and easier packing. No channeling in the bed, leading to tighter bands, thus better separation. Lower pressure drop (fewer fine particles), leading to faster flow, thus faster run time.
2. Small pore volume: Higher bulk density.

More silica gel per column volume, leading to greater loadability. Lower dead volume, leading to less solvent use.
3. Narrow pore size distribution: Minimum small pores.

Maximum targeted surface area available, leading to greater loadability. Minimum risk of product trapped in pores, leading to longer life time of the silica gel.
4. Purity: 99.95% SiO2

Our high purity (low metal impurities) ensures for maximum resolution as compared to the competition.
5. Side by side comparison tests of every major silica gel show our Premium Rf to give the best results.
Easier to pack More fractions Faster run time Better resolution
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Granular Silica Gel - MPLC, Flash & Gravity LC Phases

Granular or irregular-shaped silica gel is commonly used for the following applications: Medium Pressure Liquid Chromatography (MPLC), Flash Chromatography, and Classic Column Chromatography. Silica gels used for these applications are normally 60 or sometimes 100. For larger porosities please visit our Spherical Silica Gel pages later on in this catalog and also available online at www.sorbtech.com. For granular bonded phase silica gel, see the Bonded Phase Section.
Recommended Applications: Aflatoxins Chloramphenicol Pesticides Steroids Vitamins
Silica Gel - Granular

Premium Rf Premium Rf Premium Rf Premium Rf Standard Grade Standard Grade Standard Grade Standard Grade Standard Grade Standard Grade Standard Grade Standard Grade Standard Grade Standard Grade Standard Grade Standard Grade, Active Standard Grade, Active Standard Grade, Active Standard Grade, Active Standard Grade, Active Standard Grade, Active Standard Grade, Active
Catalog No.
52200 52300 52500 52700 11610 10910 10920 10930 30930M 40930 10940 30940 40940 10400 10950 10800 10810 10820 10830 10840 10850 10860
Particle Dist
15 - 40 m 20 - 45 m 40 - 75 m 75 - 200 m 5 - 15 m 12 - 26 m 18 - 32 m 32 - 63 m 40 - 63 m 40 - 63 m 63 - 200 m 63 - 200 m 63 - 200 m 100 - 200 m 200 - 500 m 18 - 32 m 32 - 63 m 32 - 100 m 63 - 100 m 63 - 200 m 100 - 200 m 200 - 500 m
Porosity
60 60 60 60 60 60 60 60 60 60 60 60 60 60 60 60 60 60 60 60 60 60
pH Value
7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7
LOI
5% 5% 5% 5% < 9% < 7% < 9% < 7% < 5% < 5% < 5% < 5% < 5% < 5% < 5%
Bulk Density
0.5 g/ml 0.5 g/ml 0.5 g/ml 0.5 g/ml 0.4 g/ml 0.4 g/ml 0.4 g/ml 0.4 g/ml 0.5 g/ml 0.5 g/ml 0.5 g/ml 0.5 g/ml 0.5 g/ml 0.5 g/ml 0.5 g/ml 0.4 g/ml 0.4 g/ml 0.5 g/ml 0.5 g/ml 0.5 g/ml 0.5 g/ml 0.5 g/ml
Surface Area
450-550 m2/g 450-550 m2/g 470-570 m2/g 470-570 m2/g 500-600 m2/g 500-600 m2/g 500-600 m2/g 500-600 m2/g 450-550 m2/g 450-550 m2/g 500-600 m2/g 450-550 m2/g 450-550 m2/g 500-600 m2/g 500-600 m2/g 500-600 m2/g 500-600 m2/g 500-600 m2/g 500-600 m2/g 500-600 m2/g 500-600 m2/g 500-600 m2/g -
Water Soluble Matter
0.2% 0.2% 0.2% 0.2% 0.2% 0.2% 0.2% 0.2% 0.2% 0.2% 0.2% 0.2% 0.2% 0.2%
Tip:
To standardize silica gel from chemist to chemist, lot to lot, run to run, place the amount of silica gel you are using into a vacuum oven and heat for 30-45 min. at 130C. Cool to room temperature, then pack your column. This will eliminate any residual moisture.
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MPLC Silica Gel - Granular

Sorbtech MPLC grades provide higher theoretical plates for greater resolution. When you have a very low Rf on TLC and scaling up to flash does not work, you need the resolution power of MPLC grades.
Cat. #
11610-1 11610-25 52200-1 52200-25 10910-1 10910-25 10920-1 10920-25 52300-1 52300-25
Product
Silica Gel for TLC, Standard Grade, 60 Silica Gel for TLC, Standard Grade, 60 Silica Gel for Prep. TLC, Premium Rf, 60 Silica Gel for Prep. TLC, Premium Rf, 60 Silica Gel for MPLC, Standard Grade, 60 Silica Gel for MPLC, Standard Grade, 60 Silica Gel for MPLC, Standard Grade, 60 Silica Gel for MPLC, Standard Grade, 60 Silica Gel for MPLC, Premium Rf, 60 Silica Gel for MPLC, Premium Rf, 60 5-15m 5-15m 15-40m 15-40m 12-26m 12-26m 18-32m 18-32m 20-45m 20-45m
Part. Dist.
1kg 25kg 1kg 25kg 1kg 25kg 1kg 25kg 1kg 25kg
Size
MPLC Grades - provide improved resolution for difficult separations especially those on TLC
Flash & Gravity Silica Gels - Granular

Our flash and gravity grades have been selected for high performance and optimal reproducibility * For Bonded Phases see page 12.
Cat. #
Flash Chromatography Grades - Granular 52500-1 52500-2.5 52500-5 52500-25 30930M-1 30930M-2.5 30930M-5 30930M-25 40930-25
Product
Silica Gel, Premium Rf, 60 Silica Gel, Premium Rf, 60 Silica Gel, Premium Rf, 60 Silica Gel, Premium Rf, 60 Silica Gel, Standard Grade, 60 Silica Gel, Standard Grade, 60 Silica Gel, Standard Grade, 60 Silica Gel, Standard Grade, 60 Silica Gel, Standard Grade, 60
Part. Dist.
40-75m (200x400 mesh) 40-75m (200x400 mesh) 40-75m (200x400 mesh) 40-75m (200x400 mesh) 40-63m (230x400 mesh) 40-63m (230x400 mesh) 40-63m (230x400 mesh) 40-63m (230x400 mesh) 40-63m (230x400 mesh) 1kg 2.5kg 5kg 25kg 1kg 2.5kg 5kg 25kg 25kg
Size
Cat. #
Gravity Chromatography Grades - Granular 52700-1 52700-2.5 52700-5 52700-20 30940-1 30940-2.5 30940-5 30940-25 40940-25
Product
Silica Gel, Premium Rf, 60 Silica Gel, Premium Rf, 60 Silica Gel, Premium Rf, 60 Silica Gel, Premium Rf, 60 Silica Gel, Standard Grade, 60 Silica Gel, Standard Grade, 60 Silica Gel, Standard Grade, 60 Silica Gel, Standard Grade, 60 Silica Gel, Standard Grade, 60
Part. Dist.
75-200m (70x200 mesh) 75-200m (70x200 mesh) 75-200m (70x200 mesh) 75-200m (70x200 mesh) 63-200m (70x230 mesh) 63-200m (70x230 mesh) 63-200m (70x230 mesh) 63-200m (70x230 mesh) 63-200m (70x230 mesh) 1kg 2.5kg 5kg 20kg 1kg 2.5kg 5kg 25kg 25kg
Size
Also available in smaller package sizes For more selections and sizes, visit our website or contact your personal Product Specialist. Sorbent Technologies, Inc. Toll Free: 866.SORBTEC Fax: 770.936.0326 www.sorbtech.com 7
Silica Gel for ASTM D1319-02a and ISO 3837

Quality Silica Gel
Our Silica Gel #51600 is a high purity silica and is manufactured to conform to the specifications of ASTM D1310-02a Test Method for Hydrocarbon Types in Liquid Petroleum Products by Fluorescent Indicator Adsorption & ISO 3037 Liquid Petroleum Products-Determination of Hydrocarbon TypesFluorescent Indicator Adsorption Method. Sorbtech Silica Gel #51600 can be used in place of W.R. Graces product (Code 923.) See table below.
ASTM D131-02a
This method covers the determination of hydrocarbon types over the concentration ranges from 5 to 99 volume % aromatics, 0.3 to 55 volume % olefins, and 1 to 95 volume % saturates in petroleum fractions that distill below 315C. This test method may apply to concentrations outside these ranges, but the precision has not been determined. Samples containing dark-colored components that interfere in reading the chromatographic bands cannot be analyzed.
ISO 3037
The determination of the total volume percent [% (V/V)] of saturates, olefins, and aromatics in petroleum fractions is important in characterizing the quality of petroleum fractions as gasoline blending components and as feeds to catalytic reforming processes, and in characterizing petroleum fractions and products from catalytic reforming and from thermal and catalytic cracking as blending components for motor and aviation fuels. This information is also important as a measure of the quality of aviation turbine fuels.
Cat #51600, Silica Gel, Premium Rf, 30A, 75-150um - Typical Product Specifications
Item
Year Surface Area pH Loss of Ignition Fe2O3 Particle Size Distribution on 250 m on 180 m on 150 m 75 to 150 m Thru 75 m Pore Volume H2O Laser APS SiO2 Na Mg Al Ca Fe Zr Ti Cr Mn Co Ni Zn Cd Pb ml/g % m % ppm ppm ppm ppm ppm ppm ppm ppm ppm ppm ppm ppm ppm ppm 15 max 0 1.2 max 5 max 1.2 max 0 0 0 94.4 5.6 0.39 0.66 141.23 99.95 29 0.8 5 5.1 3.8 15 102 0.18 0.02 0.17 0.06 0.2 0.02 0.13 mass % Mass ppm m2/g
Unit
ASTM D1319-02a
2002 430-530 5.5-7.0 4.5-10.0 50 max 515 5.99 4.87 -
Results
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Spherical vs. Granular

1. Physical Properties
Spherical Silica Competitor A Items
Cat. #63700 Granular Silica Unit

(m2/g) (ml/g) (g/ml) (%) - (cm) (%) m m m - g/g g/g g/g
100, 75-200m 100, 60-200m Granular

281 1.14 0.40 1.65 7.5 15,700 33 143 228 9 2.5 530 1100 60
Spherical has lower pore-volume and high bulk density Higher loading in the same column volume Spherical has high specific resistance. Low impurity content Spherical has high transparency. Low content of fine particles
Spherical
272 0.81 0.51 6.43 7.8 57,100 76 103 141 80 1.8 298 90 18
2. Particle Size Distribution
Spherical has lower mean particle size and sharper distribution Higher efficiency Lower pressure drop and easier flow rate Spherical Shape Stable chromatography bed No fine generations
3. Chemical Properties / Impurities 4. Flash Chromatography Test

Flash chromatography conditions:
Column: Sample weight: Mobile phase: Flow rate: Detection:
Surface area Pore volume Bulk density Loss on drying pH (5% suspension) Specific resistance Transparency Particle Size d50 (Average) d10 d90 d10/d90 Chemical Impurities Na Ca Fe
Spherical has lower impurity levels, especially for calcium No undesirable tailing of polar compounds
20 x 500 mm glass column 25 g 1. Benzene 2. Dimethyl Phthalate n-hexane / ethyl acetate (90-10) w% 5 cm/min (15.7 ml /min) UV 254 nm
Spherical Silica showed higher resolution. The reasons are:

Smaller particle size. Homogeneous packing due to the spherical particle shape.
5a. Adsorption Curve
Column height (mm) Retention time (DMP) min k (DMP) N (DMP) Symmetry (DMP)
Spherical 172 10.61 2.48 241 1.6
Granular 235 11.44 3.50 118 2.1
A long break through time (point A) indicates higher capacity A short mass transfer time (A-B) indicates higher equilibrium
Both silica gels were packed in 4.6 x 250 mm stainless steel columns by dry packing. Capacity of Dimethyl Phthalate (DMP): Flow rate 2 ml/min d (solvent) 0.65 g/ml or 1.3 g/min DMP (1%) 13 mg/min Capacity (mg) 13 x A
Adsorption Times
Point A Point B Segment A-B Column Capacity Silica capacity
Unit
(min) (min) (min) (mg) (mg/g)
Spherical
24.0 25.3 1.3 312 146
Granular
19.3 21.5 2.2 251 155 1.9 4.1 10.8 8.9 6.7
Elution Times
Point C (min) 2.3 Point D (min) 5.8 Point E (min) 10.5 Segment C-E (min) 8.2 Segment D-E (min) 4.7 C-E: Elution time D-E: Concentration change time (Mass transfer zone)
5b. Elution / Desorption Curve
Short mass transfer time (Short equilibrium) (D-E) indicates lower solvent consumption
6. Conclusion
When using spherical silica gel you will have

Increase loading - up to 30% more Greater reproducibility of process Sharper peaks and cost savings (less solvent consumption and labor) Longer bed lifetime (decrease cost of labor and silica consumption) Better chromatographic results without switching to costly HPLC equipment
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Spherical Silica Gel - HPLC, MPLC, Flash & Gravity LC Phases

Catalog No.
72200 73200 74200 75200 76200 77200 78200 79200 72300 73300 74300 75300 76300 77300 78300 79300 72400 73400 74400 75400 76400 77400 78400 79400 72600 73600 74600 75600 76600 77600 78600 79600 62500 63500 66500 67500 68500 69500 62700 63700 66700 67700 68700 69700 63900 64900
Particle Dist.
5 m 5 m 5 m 5 m 5 m 5 m 5 m 5 m 10 m 10 m 10 m 10 m 10 m 10 m 10 m 10 m 15 m 15 m 15 m 15 m 15 m 15 m 15 m 15 m 20-45 m 20-45 m 20-45 m 20-45 m 20-45 m 20-45 m 20-45 m 20-45 m 40-75 m 40-75 m 40-75 m 40-75 m 40-75 m 40-75 m 75-200 m 75-200 m 75-200 m 75-200 m 75-200 m 75-200 m 200-500 m 200-500 m
Porosity
70 100 150 200 300 500 800 1000 70 100 150 200 300 500 800 1000 70 100 150 200 300 500 800 1000 70 100 150 200 300 500 800 1000 70 100 300 500 800 1000 70 100 300 500 800 1000 100 150
pH Value
4.0 - 7.0 4.0 - 7.0 4.0 - 7.0 4.0 - 7.0 4.0 - 7.0 4.0 - 7.0 4.0 - 7.0 4.0 - 7.0 4.0 - 7.0 4.0 - 7.0 4.0 - 7.0 4.0 - 7.0 4.0 - 7.0 4.0 - 7.0 4.0 - 7.0 4.0 - 7.0 4.0 - 7.0 4.0 - 7.0 4.0 - 7.0 4.0 - 7.0 4.0 - 7.0 4.0 - 7.0 4.0 - 7.0 4.0 - 7.0 6.0 -8.0 6.0 -8.0 6.0 -8.0 6.0 -8.0 6.0 -8.0 6.0 -8.0 6.0 -8.0 6.0 -8.0 6.0 -8.0 6.0 -8.0 6.0 -8.0 6.0 -8.0 6.0 -8.0 6.0 -8.0 6.0 -8.0 6.0 -8.0 6.0 -8.0 6.0 -8.0 6.0 -8.0 6.0 -8.0 6.0 -8.0 6.0 -8.0
Loss on Drying
2.0% 2.0% 2.0% 2.0% 2.0% 2.0% 2.0% 2.0% 2.0% 2.0% 2.0% 2.0% 2.0% 2.0% 2.0% 2.0% 2.0% 2.0% 2.0% 2.0% 2.0% 2.0% 2.0% 2.0% 2.0% 2.0% 2.0% 2.0% 2.0% 2.0% 2.0% 2.0% 3.0 - 7.0% 3.0 - 7.0% 3.0 - 7.0% 3.0 - 7.0% 3.0 - 7.0% 3.0 - 7.0% 3.0 - 7.0% 3.0 - 7.0% 3.0 - 7.0% 3.0 - 7.0% 3.0 - 7.0% 3.0 - 7.0% 3.0 - 7.0% 3.0 - 7.0%
Bulk Density
0.45 g/ml 0.45 g/ml 0.45 g/ml 0.45 g/ml 0.45 g/ml 0.45 g/ml 0.45 g/ml 0.45 g/ml 0.45 g/ml 0.45 g/ml 0.45 g/ml 0.45 g/ml 0.45 g/ml 0.45 g/ml 0.45 g/ml 0.45 g/ml 0.45 g/ml 0.45 g/ml 0.45 g/ml 0.45 g/ml 0.45 g/ml 0.45 g/ml 0.45 g/ml 0.45 g/ml 0.45 g/ml 0.45 g/ml 0.45 g/ml 0.45 g/ml 0.45 g/ml 0.45 g/ml 0.45 g/ml 0.45 g/ml 0.5 g/ml 0.5 g/ml 0.5 g/ml 0.5 g/ml 0.5 g/ml 0.5 g/ml 0.5 g/ml 0.5 g/ml 0.5 g/ml 0.5 g/ml 0.5 g/ml 0.5 g/ml 0.5 g/ml 0.5 g/ml
Surface Area
500 m2/g 270 m2/g 175 m2/g 130 m2/g 90 m2/g 60 m2/g 45 m2/g 30 m2/g 500 m2/g 270 m2/g 175 m2/g 130 m2/g 90 m2/g 60 m2/g 45 m2/g 30 m2/g 500 m2/g 270 m2/g 175 m2/g 130 m2/g 90 m2/g 60 m2/g 45 m2/g 30 m2/g 500 m2/g 270 m2/g 175 m2/g 130 m2/g 90 m2/g 60 m2/g 45 m2/g 30 m2/g 500 m2/g 270 m2/g 90 m2/g 60 m2/g 45 m2/g 30 m2/g 500 m2/g 270 m2/g 90 m2/g 60 m2/g 45 m2/g 30 m2/g 270 m2/g 175 m2/g
Pore Volume
0.80 ml/g 0.80 ml/g 0.80 ml/g 0.80 ml/g 0.80 ml/g 0.80 ml/g 0.80 ml/g 0.80 ml/g 0.80 ml/g 0.80 ml/g 0.80 ml/g 0.80 ml/g 0.80 ml/g 0.80 ml/g 0.80 ml/g 0.80 ml/g 0.80 ml/g 0.80 ml/g 0.80 ml/g 0.80 ml/g 0.80 ml/g 0.80 ml/g 0.80 ml/g 0.80 ml/g 0.80 ml/g 0.80 ml/g 0.80 ml/g 0.80 ml/g 0.80 ml/g 0.80 ml/g 0.80 ml/g 0.80 ml/g 0.80 ml/g 0.80 ml/g 0.80 ml/g 0.80 ml/g 0.80 ml/g 0.80 ml/g 0.80 ml/g 0.80 ml/g 0.80 ml/g 0.80 ml/g 0.80 ml/g 0.80 ml/g 0.80 ml/g 0.80 ml/g
Compound Size
<1,000 Daltons <10,000 Daltons <10,000 Daltons <10,000 Daltons <10,000 Daltons <50,000 Daltons <50,000 Daltons <50,000 Daltons <1,000 Daltons <10,000 Daltons <10,000 Daltons <10,000 Daltons <10,000 Daltons <50,000 Daltons <50,000 Daltons <50,000 Daltons <1,000 Daltons <10,000 Daltons <10,000 Daltons <10,000 Daltons <10,000 Daltons <50,000 Daltons <50,000 Daltons <50,000 Daltons <1,000 Daltons <10,000 Daltons <10,000 Daltons <10,000 Daltons <10,000 Daltons <50,000 Daltons <50,000 Daltons <50,000 Daltons <1,000 Daltons <10,000 Daltons <10,000 Daltons <50,000 Daltons <50,000 Daltons <50,000 Daltons <1,000 Daltons <10,000 Daltons <10,000 Daltons <50,000 Daltons <50,000 Daltons <50,000 Daltons <10,000 Daltons <10,000 Daltons
10
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HPLC, MPLC Silica Gel- Spherical

High purity silica gel where metal ions and salt concentration is typically low. Various particle diameters and pore sizes allow for easy transition from analytical to preparative. These silica gels can be used as the base materials for functionalization (eg. C18, C8, Amino, etc.)
HPLC, Prep HPLC, and MPLC Chromatography Grades - Spherical

Cat. #
72200-001 73200-001 74200-001 75200-001 76200-001 77200-001 78200-001 79200-001 73300-001 73400-001 72600-05 72600-1 72600-2.5 72600-5 72600-20 73600-01
Product
Spherical Silica Gel, Premium Rf, 70 Spherical Silica Gel, Premium Rf, 100 Spherical Silica Gel, Premium Rf, 150 Spherical Silica Gel, Premium Rf, 200 Spherical Silica Gel, Premium Rf, 300 Spherical Silica Gel, Premium Rf, 500 Spherical Silica Gel, Premium Rf, 800 Spherical Silica Gel, Premium Rf, 1000 Spherical Silica Gel, Premium Rf, 100 Spherical Silica Gel, Premium Rf, 100 Spherical Silica Gel, Premium Rf, 70 Spherical Silica Gel, Premium Rf, 70 Spherical Silica Gel, Premium Rf, 70 Spherical Silica Gel, Premium Rf, 70 Spherical Silica Gel, Premium Rf, 70 Spherical Silica Gel, Premium Rf, 100 5m 5m 5m 5m 5m 5m 5m 5m 10m 15m
Part. Dist.
10g 10g 10g 10g 10g 10g 10g 10g 10g 10g 500g 1kg 2.5kg 5kg 20kg 100g
Size
20-45m 20-45m 20-45m 20-45m 20-45m 20-45m
Flash & Gravity Silica Gel- Spherical

Our flash and gravity grade silicas are ideal for flash chromatography, industrial scale LC, and batch adsorption applications. Sorbtech Flash and Gravity silicas function through hydrophilic interactions with more polar compounds generally retained longer, making them ideally suited for the purification of chemical synthesis intermediates, oils, fats, and natural products (vitamins, flavors, fragrances, etc.)
Flash & Gravity Chromatography Grades - Spherical

Cat. #
62500-01 62500-05 62500-1 62500-5 62500-20 62700-01 62700-05 62700-1 62700-5 62700-20
Product
Spherical Silica Gel, Premium Rf, 70 Spherical Silica Gel, Premium Rf, 70 Spherical Silica Gel, Premium Rf, 70 Spherical Silica Gel, Premium Rf, 70 Spherical Silica Gel, Premium Rf, 70 Spherical Silica Gel, Premium Rf, 70 Spherical Silica Gel, Premium Rf, 70 Spherical Silica Gel, Premium Rf, 70 Spherical Silica Gel, Premium Rf, 70 Spherical Silica Gel, Premium Rf, 70
Part. Dist.
40-75m (200x400 mesh) 40-75m (200x400 mesh) 40-75m (200x400 mesh) 40-75m (200x400 mesh) 40-75m (200x400 mesh) 75-200m (70x200 mesh) 75-200m (70x200 mesh) 75-200m (70x200 mesh) 75-200m (70x200 mesh) 75-200m (70x200 mesh) 100g 500g 1kg 5kg 20kg 100g 500g 1kg 5kg 20kg
Size
For more selections and sizes, visit our website or contact your personal Product Specialist. Sorbent Technologies, Inc. Toll Free: 866.SORBTEC Fax: 770.936.0326 www.sorbtech.com 11
Bonded Phase Silica Gels - Spherical

Sorbtech bonded phases start with Premium Rf silica gel as the base material to ensure the greatest purity and performance for all your laboratory needs. This highest grade silica ensures optimum resolution for even the most difficult separations. Manufactured to strict standards, Premium Rf bonded phases provide uniform porosity and pore volume for high loadability. The exceptional surface coverage of the alkylsilanes and endcapping techniques provide a surface with the lowest silanol activity. Bonded phases are available in HPLC, MPLC, Flash, and Gravity grades. Packaging sizes available in 10g, 100g, 500g, 1kg, 2.5kg, 5kg and 25kg. For more selections and sizes, visit our website or contact a Product Specialist.
Phases and Their Functions

C18 - Octadecyl
Most popular and versatile reversed phase. Very non-polar, hydrophobic interactions with a wide variety of organic compounds. We offer the following C18 options: C18 Standard Bonding with Endcapping. Excellent for routine analysis. C18AQ - Stable in 100% aqueous Mobile phase systems. C18AQ2 Offers 100% aqueous stability designed to match the retention times with leading aqueous C18 brands. C18DE Dual Encapping allows for enhanced pH stability 1 12.
C8 - Octyl
Similar to C18, but the shorter alkyl chains cause more polar secondary interactions with polar compounds. Less retentive than C18. We offer two C8 options: C8 Standard bonding with endcapping C8DE Dual Encapping allows for enhanced pH stability 1 12.
C4 - Butyl
More polar than C18 and C8 because it has short alkyl chains and the silica surface is not completely shielded. Used for compounds that are highly retained on C18 or C8.
C2 - Ethyl
Weakly non-polar. Plasma, urine, and aqueous samples.
Amino
Will separate a variety of sugars with acetonitrile / water mobile phase. With hexane / ethyl acetate mobile phase, it will function as an alternative to bare silica during normal phase chromatography and offer a different retention time. In addition, since the surface contains primary amino groups, this phase can be used as a base for coupling chiral ligands. Also can be used as a weak anion exchanger.
Cyano
Can be used in both normal and reversed phase. Popular for purification of natural products.
Phenyl Diol
Specific reversed phase for medium / polar molecules and aromatics, shows a high selectivity for acceptor / donor compounds.
Particularly used for aqueous GPC applications. Diol can also be used for normal phase. DIOL can function as an alternative stationary phase during normal phase chromatography and offer a different retention time than either bare silica gel or amino bonded silica gel due to its neutral charge.
WCX
Carboxylic Acid (COOH), weak cation exchanger
SCX
Propyl Sulfonic Acid (HSO3), strong cation exchanger
SH (Thiol)
Scavenger of electrophiles (eg. alkyl-, benzyl, allyl- halides, acid chlorides, isocyanates) Metal scavenger (eg. Pd, Pt, Cu, Hg, Ag, Pb)
HILIC A
Butyl-amine-carboxylic acid type, ideal for Amino Acids & Small Peptides, etc.
HILIC B
Diamine-carboxylic acid type, ideal for Nucleic Acids, Organic Acids, etc.
12
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Bonded Phase Silica Gels-Spherical cont.

Cat. #
52516 52526 52546M 62526 62546 62560 63505 63516 63526 63527 63546 63547 63548 63549 63554 63560 63566 63570 63577 63580 63590 63591 63716 63726 63746 63754 63760 63765 63770 63785 66526 66546 66555 72246 72346 73215 73226 73246 73255 73260 73265 73270 73326 73346 73626
Product
C4 Silica Gel, Premium Rf, 60, End-capped, Carbon Load: 5-7% C8 Silica Gel, Premium Rf, 60, End-capped, Carbon Load: 8-12% C18 Silica Gel, Premium Rf, 60, End-capped, Carbon Load: 18-22% C8 Spherical Silica Gel, Premium Rf, 70, End-capped, Carbon Load: 8-12% C18 Spherical Silica Gel, Premium Rf, 70, End-capped, Carbon Load: 18-24% CN Spherical Silica Gel, Premium Rf, 70, End-capped, Carbon Load: 5-7% C2 Spherical Silica Gel, Premium Rf, 100, End-capped, Carbon Load: 2-4% C4 Spherical Silica Gel, Premium Rf, 100, End-capped, Carbon Load: 3-5% C8 Spherical Silica Gel, Premium Rf, 100, End-capped, Carbon Load: 6-9% C8DE Spherical Silica Gel, Premium Rf, 100, Dual End-capped, Carbon Load: 6-9% C18 Spherical Silica Gel, Premium Rf, 100, End-capped, Carbon Load: 15-19% C18DE Spherical Silica Gel, Premium Rf, 100, Dual End-capped Carbon Load: 15-19% C18 Aqueous, Spherical Silica Gel, 100, Carbon Load: 9.0 - 13.3% C18 Aqueous II, Spherical Silica Gel, 100, Carbon Load: 9.0 - 13.3% NH2 Spherical Silica Gel, 100, Carbon Load: 3-5% CN Spherical Silica Gel, Premium Rf, 100, End-capped, Carbon Load: 3-5% Phenyl Spherical Silica Gel, Premium Rf, 100, End-capped, Carbon Load: 10-14% Diol Spherical Silica Gel, Premium Rf, 100, WCX (weak cation exchanger) Spherical Silica Gel, Premium Rf, 100 SCX (strong cation exchanger) Spherical Silica Gel, Premium Rf, 100 HILIC A Spherical Silica Gel, Premium Rf, 100 HILIC B Spherical Silica Gel, Premium Rf, 100 C4 Spherical Silica Gel, Premium Rf, 100, End-capped, Carbon Load: 3-5% C8 Spherical Silica Gel, Premium Rf, 100, End-capped, Carbon Load: 6-9% C18 Spherical Silica Gel, Premium Rf, 100, End-capped, Carbon Load: 15-19% NH2 Spherical Silica Gel, Premium Rf, 100, Carbon Load: 3-5% CN Spherical Silica Gel, Premium Rf, 100, End-capped, Carbon Load: 3-5% Phenyl Spherical Silica Gel, Premium Rf, 100, End-capped, Carbon Load: 10-14% Diol Spherical Silica Gel, Premium Rf, 100, Thiol (Scavenger) Spherical Silica Gel, Premium Rf, 100 C8 Spherical Silica Gel, Premium Rf, 300, End-capped, Carbon Load: 2-5% C18 Spherical Silica Gel, Premium Rf, 300, End-capped, Carbon Load: 4.5-8.5% NH2 Spherical Silica Gel, Premium Rf, 300, Carbon Load: 1-2% C18 Spherical Silica Gel, Premium Rf, 70A, End-capped, Carbon Load: 18-22% C18 Spherical Silica Gel, Premium Rf, 70A, End-capped, Carbon Load: 18-22% C4 Spherical Silica Gel, Premium Rf, 100A, End-capped, Carbon Load: 3-5% C8 Spherical Silica Gel, Premium Rf, 100A, End-capped, Carbon Load: 8-12% C18 Spherical Silica Gel, Premium Rf, 100A, End-capped, Carbon Load: 16-20% NH2 Spherical Silica Gel, Premium Rf, 100A, Carbon Load: 3-5% CN Spherical Silica Gel, Premium Rf, 100A, End-capped, Carbon Load: 3-5% Phenyl Spherical Silica Gel, Premium Rf, 100A, End-capped, Carbon Load: 10-14% Diol Spherical Silica Gel, Premium Rf, 100A, C8 Spherical Silica Gel, Premium Rf, 100A, End-capped, Carbon Load: 6-9% C18 Spherical Silica Gel, Premium Rf, 100A, End-capped, Carbon Load: 16-20% C8 Spherical Silica Gel, Premium Rf, 100A, End-capped, Carbon Load: 6-9%
Part. Dist.
40-75m 40-75m 40-63m 40-75m 40-75m 40-75m 40-75m 40-75m 40-75m 40-75m 40-75m 40-75m 40-75m 40-75m 40-75m 40-75m 40-75m 40-75m 40-75m 40-75m 40-75m 40-75m 75-200m 75-200m 75-200m 75-200m 75-200m 75-200m 75-200m 75-200m 40-75m 40-75m 40-75m 5m 10m 5um 5um 5um 5um 5um 5um 5um 10um 10um 20-45um
Available in additional package sizes.
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13
Alumina
Alumina
Sorbent Technologies aluminas for column chromatography are the best available. A stringent manufacturing process yields standardized aluminas which are measured according to Brockmann methods. This ensures precise specifications and lot to lot reproducibility. Our aluminas are ideal for natural product extraction, purification & de-colorization, heavy metal removal, cleanup, and more. We offer the broadest selection of standardized aluminas with defined pH values (acid, base, neutral), specific activity levels (Super I, Act. I, II, III, IV, V) and numerous particle distributions (MPLC, Flash, Gravity). These aluminas can be adjusted to lower activity by the addition of polar media, preferably water. See Deactivation Procedure on next page.
Typical Applications for Aluminas

Acidic Neutral Basic
Super I
Food dyestuffs Determination of Morphine Determination of diphenyl
Super I
Ketosteroids Glycosides Vitamins Removal of pyrogens Dehydration of organic solvents Alkaloids
Super I
Purification of organic solvents Synthetic bases Dehydration of ethers Removal of pyrogens Natural and Synthetic dyestuffs Hydrocarbons
Standard Activity I
Plant Extraction Fatty Acids Plant Waxes Azobenzene
Standard Activity I
Antibiotics Essential Oils Plant extraction Enzymes Glycosides Hormones Diesel exhaust Pharmaceutical Compounds Fly agaric
Standard Activity I
Alkaloids Essential Oils Plant extraction Purification of organic solvents Insecticides Clarification of fatty oils Removal of alcohol from chloroform
Alumina
pH approx.
4.5 7.5 10 4.5 7.5 10 10 7.4 10 4.3
Activity
LOI approx.
1.3% 1% 1% 1.7% 1.7% 1.7%
Particle Dist.
50-200m 50-200m 50-200m 50-200m 50-200m 50-200m 50-200m 32-63m
Water Sol. Matter approx

0.1% 0.1% 0.1% 0.1% 0.1% 0.1% 0.2% 0.1%
Bulk Density approx.

0.8g/ml 0.8g/ml 0.8g/ml 0.8g/ml 0.8g/ml 0.8g/ml 0.8g/ml 0.8/ml 0.8g/ml 0.8g/ml
Surface Area approx.

200 m2/g 200 m2/g 200 m2/g 150 m2/g 150 m2/g 150 m2/g 200m2/g 200m2/g
Super I - Acidic Super I - Neutral Super I - Basic Standard Act. I - Acidic Standard Act. I - Neutral Standard Act. I - Basic Act. II-III Alumina N for Flash Chromatography Alumina B - Super I for Dioxin Analysis Alumina R
Super I Super I Super I Act. I Act. I Act. I Act. II-III
0.7%
50-200m 50-200m
14
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Deactivation of Alumina
Deactivation allows the chromatographer the flexibility and freedom to deactivate alumina to the precise activity level required for their specific application. Alumina may be deactivated by the addition of polar media, preferably water, but also alcohols such as glycol or glycerol can be used. Sorbent Technologies standardized aluminas are based on a deactivation scale. Alumina Super I is standardized to such a high degree that all three surface types of Aluminas, (acid, basic, neutral) show an identical initial activity, and when deactivated with equal amounts of deactivator (e.g. water), they reach the same lower activity status. The percent of water addition is based upon weight/weight relationships. Activity level is according to Brockmann.
Deactivation Procedure:
1. Weigh a known quantity of alumina and place into a tightly sealed vessel 2. Add the required amount of water (by weight or volume) 3. Shake the mixture in the closed vessel until all lumps disappear 4. Allow the mixture to cool (preferably overnight) 5. The container should remain tightly closed to retain activity level and can be stored for later use
The amount of water needed for deactivation can be read off the graph below:
Activity
Super I Std. Act. I 0
Super I
1 0 N/A
I
4 3
II
7 6
III
10 10
IV
19 15
Activity Level According to Brockmann

% water to add % water to add
Alumina, Super I
Alumina Activity Super I is designed for the separation of polar samples in non-solvent systems as well as the purification and dehydration of solvents for HPLC and other analytical techniques. In a non-polar environment, Alumina Activity Super I shows sorption capacity approximately twice as high as that of Alumina Standard Activity I. Their initial activity is kept in a very narrow range and their deactivation is extremely constant.
Cat. #
15100-1 15100-5 15130-1 15130-5 15160-1 15160-5
Product
Alumina, Super I - Acidic Alumina, Super I - Acidic Alumina, Super I - Neutral Alumina, Super I - Neutral Alumina, Super I - Basic Alumina, Super I - Basic
Part. Dist.
50-200m (70x270 mesh) 50-200m (70x270 mesh) 50-200m (70x270 mesh) 50-200m (70x270 mesh) 50-200m (70x270 mesh) 50-200m (70x270 mesh) 1kg 5kg 1kg 5kg 1kg 5kg
Size
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15
Alumina, Standard Act. I

Alumina, Standard Activity I is similar to the Alumina, Activity Super I in that it is also standardized. The starting activity level is about half as high and can be deactivated according to the deactivation table on the previous page. Standardized manufacturing processes provide a specific Brockmann dryness level for lot to lot reproducibility and precise deactivation possibilities.
Cat. #
15600-1 15600-5 15600-25 15600-50 15740-1 15740-5 15740-25 15740-50 15640-1 15640-5 15640-25 15640-50
Product
Alumina, Standard Act. I - Acidic Alumina, Standard Act. I - Acidic Alumina, Standard Act. I - Acidic Alumina, Standard Act. I - Acidic Alumina, Standard Act. I - Neutral Alumina, Standard Act. I - Neutral Alumina, Standard Act. I - Neutral Alumina, Standard Act. I - Neutral Alumina, Standard Act. I - Basic Alumina, Standard Act. I - Basic Alumina, Standard Act. I - Basic Alumina, Standard Act. I - Basic
Part. Dist.
50-200m (70x270 mesh) 50-200m (70x270 mesh) 50-200m (70x270 mesh) 50-200m (70x270 mesh) 50-200m (70x270 mesh) 50-200m (70x270 mesh) 50-200m (70x270 mesh) 50-200m (70x270 mesh) 50-200m (70x270 mesh) 50-200m (70x270 mesh) 50-200m (70x270 mesh) 50-200m (70x270 mesh) 1kg 5kg 25kg 50kg 1kg 5kg 25kg 50kg 1kg 5kg 25kg 50kg
Size
Alumina, Activity II-III

Alumina, Act. II-III is an economical adsorbent of medium activity. Alumina, Act. II-III is often used when activated charcoal cannot be used due to its organic nature or where the cation exchange properties of basic alumina are desired. It is suitable for the development of separation methods and technical separations where a custom-made adsorbent is not available.
Cat. #
15300-1 15300-5 15300-50
Product
Alumina, Activity II-III Alumina, Activity II-III Alumina, Activity II-III
Part. Dist.
50-200m (70x270 mesh) 50-200m (70x270 mesh) 50-200m (70x270 mesh) 1kg 5kg 50kg
Size
Activated Alumina for Large Scale Purification

Alumina for Large Scale Purification is a granular activated alumina that has a high surface area and a large pore volume.
Cat. #
85800-100 85801-100 85813-100 85825-100
Product
Activated Alumina for Water/Process Liquids Clean-up Activated Alumina for Water/Process Liquids Clean-up Activated Alumina for Purification Activated Alumina for Purification
Part. Dist.
< 0.5mm 0.3 - 1mm 1 - 3mm 2 - 5mm 100kg 100kg 100kg 100kg
Size
Other Aluminas
Alumina, Neutral for Flash Chromatography has a finer particle distribution than the Super I and Act. I aluminas for sharper separations. Other
finer cuts are also available.
Alumina B Super I for Dioxin Analysis was specially developed to meet the high recovery level required for the analysis of polychlorinated
dibenzodioxins and dibenzofurans according to VDI-Regulation 3499, part I.
Alumina R is an acidic alumina developed for use in isotope chemistry. It is used to produce technetium generators or technetium cows. One example
of its application is the determination of catecholamines.
Cat. #
16050-1 16050-5 16050-25 19600-05 19650-05
Product
Alumina, Neutral for Flash Chromatography Alumina, Neutral for Flash Chromatography Alumina, Neutral for Flash Chromatography Alumina B - Super I for Dioxin Analysis Alumina R for the Technique of Isotopes
Part. Dist.
32-63m (230x450 mesh) 32-63m (230x450 mesh) 32-63m (230x450 mesh) 50-200m (70x270 mesh) 50-200m (70x270 mesh) 1kg 5kg 25kg 500g 500g
Size
For more selections and sizes, visit our website or contact your personal Product Specialist 16 Sorbent Technologies, Inc. Toll Free: 866.SORBTEC Fax: 770.936.0326
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Alumina Spheres
Sorbtechs activated alumina spheres are very porous. They have a high surface area, which allows adsorption of gases and liquids, and do not swell. They will not soften or disintegrate when immersed in liquids. When used to adsorb water, they act as an efficient desiccant. Molecules with the highest polarity are preferentially adsorbed. In the absence of irreversible fowling, they may be regenerated to their original adsorption efficiency by heating to a temperature between 350-600F (177-316C). For the most efficient subsequent adsorption, high temperature, low pressure and dry inert gas regeneration is recommended.
Benefits:
Especially important in high pressure gas dehydration for reduction of pressure drop Minimizes channeling Yields higher efficiencies Allows rapid pneumatic loading Enables use of tall towers Effective for the dehydration of acid containing gases and liquids, such as CO2 Longer operating life
Adsorption Isotherm for Alumina Spheres @25C
3. Low Abrasion
4. High Adsorptive Capacity
Ensures less dusting during High surface area transport, loading, and service life Tailored pore distribution Reduces downstream valve and Achieve an ultra low H2O effluent filter plugging
Product Applications: Process stream purification

Removal of highly polar compounds such as: TBC, alcohols, ethers, etc.
Wt. % H20 Adsorbed
1. Uniform ball size
2. High Crush Strength
Acid removal Drying
% Relative Humidity
Our alumina spheres can remove the acids formed from the degradation of trans-former oils, lubricating oils, and refrigerants. Also, they remove the residual halides and water during the manufacture of chlorinated and/or fluorinated hydrocarbons, yielding a non-corrosive product. Nearly all gases and liquids can be dried. Water removal is often necessary for efficient processing, storage, transportation or usage of fluids. For example, water can form hydrates with hydrocarbons, simulating ice, and will plug equipment.
Substances dried with alumina spheres:

Gases
Acetylene Air Ammonia CO2 Chlorine Ethane Ethylene Furnace gas Helium Hydrogen Hydrogen chloride Hydrogen sulfide Methane Natural Gas Nitrogen Oxygen Propane Propylene Refrigerants Sulfur dioxide Benzene Butane Butene Butyl acetate Carbon tetrachloride Chlorobenzene
Liquids
Cyclohexane Ethyl acetate Gasolines Heptane Hexane Hydraulic oils Jet fuel Kerosene Lubricating oils Naptha Nitrobenzene Pentane Pipe line products Propane Propylene Refrigerants Styrene Toluene Transformer oil Vegetable oil Xylene
Characteristics
Sphere size (english) Sphere size (metric) Surface Area - m2/g Total Pore Volume - cc/g Packed Bulk Density - lb/ft (kg/m )
3 3
92000
7x14 Tyler Mesh 2.0mm 360 0.5 48 (769) 11 (5) 0.1 8 22 42 1/8
92010
3/16 3.2mm 355 0.5 48 (769) 30 (14) 0.1 8 22 42
92020
1/4 6.4mm 325 0.5 4.8mm 340 0.5 48 (769) 55 (25) 0.1 7 21 40
92030
48 (769) 70 (32) 0.1 7 19 38
Crush Strength - lb (kg) Abrasion loss - wt% Static sorption at 11% RH Static sorption at 58% RH Static sorption at 97% RH
Available in 1kg, 5kg, 25kg and 50kg packaging Sorbent Technologies, Inc. Toll Free: 866.SORBTEC Fax: 770.936.0326 www.sorbtech.com 17
Alumina - Solvent Purification

Solvent Purification Methods:
Optically pure solvents are essential for spectroscopy below 300nm. Classical methods of purifying solvents for UV-Spectroscopy are very timeconsuming and complex. This is not the case when using adsorption filtration. Optically pure solvents can be quickly and easily prepared from solvents of ordinary analytical purity. Moreover, they are less sensitive to air and thus more stable.
Purification of Hydrocarbon Solvents

1. 300ml analytical grade n-hexane or pre-purified (by shaking with sulfuric acid) technical grade cyclohexane is simply filtered without suction through a column (25mm I.D. and 250mm height) filled with 150g Alumina Basic, Act. I (Cat.# 15640). The filtrate can immediately be used. Silica Gel, Active, 60 63-200m
2. Technical grade cyclohexane, that is not pre-purified, can easily be purified by filtering through a combined Silica Gel, Active, 60, 63-200m / Alumina Basic, Act. I column. A column (25mm I.D. and 400mm height) is first filled with 100g Alumina Basic, Act. I (Cat.# 15640) and on top of this,100g of Silica Gel, Active, Alumina, Basic, 60, 63-200m (Cat.# 10840) is added. Then the crude cyclohexane is dripped through. Act. I, 50-200m
Purification of Organic Solvents

Depending on the kind of impurities, which depends on the origin and the nature of the solvent, the solvent can be filtered through a column filled with Silica Gel, Active, 60, 63-200m, Alumina (Acid, Neutral or Basic), Act. I, or a combination of the two with the alumina section of the column above the silica gel section of the column. The following table gives recommendations on the amount and type of adsorbent to be used as well as the solvent yield and obtained purity.
Solvent
n-Pentane
Alumina, Acid, Act. I Cat.# 15600
Alumina, Neutral, Act. I Cat.# 15740
Alumina, Basic, Act. I Cat.# 15640

40g
Silica Gel, Active, 60, 63-200m Cat.# 10840

40g 40g
Solvent Yield (ml)

250 55 250 50 700 80 500 70 250 1600 255 255
50% Trans. at nm (4cm cuvette)

217 217 218 233 234 224 232 226 224 255 255 280 276 Alumina, (Acid, Neutral or Basic), Act. I, 50-200m Silica Gel, Active, 60, 63-200m
n-Hexane 40g Cyclohexane 40g n-Heptane 40g Isooctane 40g 40g 40g Carbon tetrachloride 40g Chloroform DAB6 40g Nitromethane Dimethylsulfoxide Pyridine 40g 40g 20g
G. Hesse and H. Schildknecht, Angew. Chem. 67, 737 (1955)
40g 40g 40g 40g
40g 40g 40g 40g 40g
80 4000 340 100 40 170 30 35 30
402 326 321 332 316
Alumina, (Acid, Neutral or Basic), Act. I, 50-200m + Silica Gel, Active, 60, 63-200m
20g
25
G. Hesse, B. P. Engelbrecht, H. Engelbrecht and S. Nitsch, Z. Anal. Chem. 241, 91 (1968)
18
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Specialty Adsorbents
Polyamide
Polyamide is well suited for the separation of natural substances with phenolic and polyphenolic groups. The chromatography of phenolic compounds on polyamide has been frequently applied for the isolation and structure determination of different natural products. Carboxylic acids are also bonded to polyamide via hydrogen bonds. The affinity for monocarboxylic is still low. Dicarboxylic acids and aromatic acids are retained stronger, especially if the aromatic part of the molecule is larger. Strong affinity for polyamide is found with numerous aromatic nitro compounds. The interaction between the nitro compound and polyamide corresponds to a reaction of a Lewis acid with a base. This is why separations of aromatic nitro compounds on polyamide can be considered as ion exchange. For this reason elution in sharp bands requires an eluent with good buffering properties. This principle has been successfully applied for the separation of dinitrophenylamino acids, which are formed in the determination of amino terminals of peptides and proteins, on polyamide columns. Quinones are irreversibly bonded to polyamide due to the free amino groups of the adsorbent.
Polyamides exhibit constant selectivities towards:

Phenols, aromatic nitro and amino compounds Chalcones, chinones, flavones Carbonic acids and their amides Anthraquinones DNP-amino acids Sulfonic acids and their amides
Cat. #
26500-025 26500-1 26520-025 26520-1 26530-025 26530-1 26530-5 26540-1 Polyamide for TLC Polyamide for TLC
Product
2-20m 2-20m <70m <70m
Part. Dist.
250g 1kg 250g 1kg 250g 1kg 5kg 1kg
Size
Polyamide for Flash Chromatography Polyamide for Flash Chromatography Polyamide for Column Chromatography Polyamide For Column Chromatography Polyamide For Column Chromatography Polyamide for Process
50-160m 50-160m 50-160m 100-300m
Bulk sizes are available
Florisil
Florisil is a very hard granular magnesia silica gel. MgO 15.5 0.5% SiO2 84.0 0.5% Na2SO4 1.0% Applications include clean-up of pesticide residues, separation of chlorinated pesticides, isolation of steroids, sex hormones, isolation of antibiotics, and separation of lipids.
Cat. #
09410-05 09415-05 09420-05 09420-3 09425-05 09425-3 09430-05 09500-05 09500-3
Product
Florisil - A Grade, Activated at 650 C Florisil - A Grade, Activated at 650 C Florisil - A Grade, Activated at 650 C Florisil - A Grade, Activated at 650 C Florisil - A Grade, Activated at 650 C Florisil - A Grade, Activated at 650 C Florisil - A Grade, Activated at 650 C Florisil - Pesticide Residue Grade Florisil - Pesticide Residue Grade
Part. Dist.
16x30 mesh 30x60 mesh 60x100 mesh 60x100 mesh 100x200 mesh 100x200 mesh -100 mesh 60x100 mesh 60x100 mesh 500g 500g 500g 3kg 500g 3kg 500g 500g 3kg
Size
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19
Dry Column Chromatography

Dry Colum Chromatography (DCC) is a fast, easy, and efficient method for separating and/or purifying industrial quantities of compounds. DCC links analytical thin layer chromatography (TLC) with preparative column chromatography (CC). Like TLC, DCC is a non-elution method utilizing Nylon Foil Tubing as a support and is available with UV-254 indicator. In TLC solvent flow is by capillary action going against gravity whereas in DCC, solvent flow is by gravity. Like CC, columns of most any size may be filled in DCC to separate or clean up very large quantities of materials. Both silica and alumina sorbents are available for DCC. Nylon Foil Tubing is solvent resistant and available in 1 - 6 diameters.
Product
Silica for DCC Alumina for DCC 7 7
pH
III III
Activity*
Fluorescent Indicator
0.5% 0.5%
Bulk Density approx.

0.5 g/ml 0.8 g/ml
Surface Area approx.

500-600 m2/g 200 m2/g
*Activity according to Brockmann
DCC Experiment
DCC is a non-elution method of column chromatography, meaning, the separated substances remain in the column at the end of the chromatographic process. The use of nylon foil tubing allows for the removal of these separated substances by easily cutting the tubing into segments.
Preparation of the column

The nylon foil tubing comes in a roll of various widths in lengths of 100ft. See table on next page. Cut the tubing to the desired length. Allow an extra 10 cm for sealing the end. Close one end of the foil by sealing in the flame of a gas lighter, folding and stapling, tying up, or any other suitable method. To remove the folds of the foil, fill one side of the tubing with very hot water, rinse with acetone, and dry with hot air. Insert a pad of cotton or glass wool into the closed end of the tubing and pierce several times with a needle to allow air to escape while the solvent migrates through the column.
Filling the column

Carefully fill the tubing, adding small quantities at a time, with Silica DCC, Alumina DCC, or sorbent that has been conditioned with the solvent (mixture). After each small quantity filled, the column should be gently tapped on a hard surface or compacted with a vibrator. Upon filling completion, the column will be so rigid that it will hold itself upright and may be fixed with a simple laboratory clamp to a stand.
Preparation of the sample

A few mililiters of a sample solution are added to about 1 g of the sorbent. The solvent is then evaporated using a rotation evaporator until sample and sorbent form a free flowing powder.
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Charging the column

The adsorbate of the sample is applied as a level layer on the top of the column. This layer is covered with about 2 cm of pure sorbent. If a concentrated sample solution is to be applied, care should be taken to achieve a starting zone, as narrow as possible. Applying liquid sample causes more uneven zones than applying the sample as an adsorbate. After penetration of the solution into the top of the column filling, about 2 cm of pure sorbent will be applied.
Development of the column

The solvent (mixture) that formed the best results in the preliminary TLC experiments is carefully added to the top of the column by means of a separatory funnel. The solvent should migrate slowly and steadily through the column. A constant liquid head of about 1 - 2 cm gives the best results. A constant liquid head is attained by placing the end of the stem of the topside closed separatory funnel about 1 cm over the top of the column. Depending on the length of the column, the viscosity of the solvent (mixture), and the height of the liquid head, the development of the column should be finished within 15 to 30 minutes. As with TLC, the components of the initial sample mixture should be separated from each other on the column. The solvent volume applied to the top of the column shouldnt exceed the amount which is necessary to fill the void fraction of the adsorbent bed.
Recovery of the separated compounds

After completing development, the column is immediately placed flat on an appropriate cutting surface. The separated zones are marked on the column, using a UV light if necessary. The column is then cut into sections with a sharp knife. The column may be cut into small slices without sorbent crumbling. The cut slices are placed into Bchner funnels and extracted and processed by appropriate methods.
Cat. #
14400-05 14400-3 14400-25 19500-05 19500-5 19500-50 NT-1 NT-1.5 NT-2 NT-2.5 NT-6
Product
Silica for DCC, w/UV254, 60 Silica for DCC, w/UV254, 60 Silica for DCC, w/UV254, 60 Alumina for DCC, w/UV254 Alumina for DCC, w/UV254 Alumina for DCC, w/UV254 Nylon Tubing for DCC Nylon Tubing for DCC Nylon Tubing for DCC Nylon Tubing for DCC Nylon Tubing for DCC
Part. Dist.
63-200m (70x230 mesh) 63-200m (70x230 mesh) 63-200m (70x230 mesh) 50-200m (70x270 mesh) 50-200m (70x270 mesh) 50-200m (70x270 mesh) 500g 3kg 25kg 500g 5kg 50kg 1 x 100 1.5 x 100 2 x 100 2.5 x 100 6 x 100
Size
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21
Polymeric Resins
Mitsubishi Polymer Resins - Ion Exchange, Purification
We offer an amazing selection of Polymer Resins from Mitsubishi (PSDVB). For over 60 years Mitsubishi has been manufacturing ion exchange resins with excellent performance, superior physical and chemical properties, and lot-to-lot reproducibility for lab and industrial separation, extraction and purification applications. Polymer resins are a series of products based on ion-exchange resin manufacturing technology and are specifically designed as a solid extractant. Synthetic adsorbents have large surface areas and fine pore structures inside the particle similar to activated carbon. Because of this characteristic, these resins can effectively adsorb organic compounds from aqueous solutions. Polymer resin adsorbents are stable in either acidic or alkaline solutions as well as organic solvents. These resins can also be easily regenerated for repeat use, increasing product life. Extraction processes with synthetic adsorbents reduce solvent usage and provide safer operations compared to conventional solvent extraction techniques. These resins are pH stable from 1-14, available in from 10um to 1000um particle sizes, wide array of pore sizes, and have a long lifetime. Packaging sizes from 10g to 200L.
Synthetic Adsorbents Aromatic Type: Diaion - HP20, HP20SS, SP20SS, Sepabeads - SP825 Food Grade Aromatic Type: Sepabeads - SP70, SP700, SP710 Modified Aromatic Type: Sepabeads - SP207, SP207SS Methacrylic Type: Diaion HP2MG, SP2MGS
HP20 synthetic adsorbent resin is a macroporous styrenic polymeric bead type resin designed for adsorption/desorption process scale applications. Its matrix provides an aromatic non-polar surface with excellent selectivity for hydrophobic areas of molecules, including biomolecules like antibiotics via low energy Van der Waals interactions. It is remarkable for its wide pore polymeric structure which provides excellent broad spectrum adsorption characteristics. HP20SS and SP20SS are smaller size versions of HP20. The wide pore polymer matrix provides excellent kinetics and capacity for small biomolecules of both preparative and process scale. They offer a nice balance of pressure flow characteristics and true chromatographic fractionation and have also been successfully applied in simulated moving bed (SMB) applications for a variety of small biomolecules. They often compete with bonded silica supports for preparative and industrial applications offering a longer lifetime. The Sepabeads SP825 resin provides a smaller more uniform pore size distribution and higher surface area as compared to Diaion HP20. The increase in surface area is approximately two times that of traditional adsorbents, and nearly matches the surface area of activated carbon. In many applications, this means twice the working capacity for the same volume of resin. They are recommended for industrial chemical process applications, bio/pharmaceutical applications such as desalting and extraction, and waste treatment. SP70, SP700, and SP710 are premium grade resins designed for the debittering of juices and related food products. These high surface area resins offer excellent kinetics and high capacity for naringin (and other bittering agents). They meet the compositional requirements for Secondary Direct Food Additives (21 CFR Section 173.65). SP70 and S710 are provided pre-conditioned to meet the extractive requirements as of the date of manufacture. SP207 resin is a macroporous chemically modified brominated styrenic polymeric type resin. The bromination of the aromatic ring provides increased hydrophobicity, and consequently, increased selectivity for hydrophobic molecules versus conventional styrene/DVB synthetic adsorbents like Diaion HP20 resin. In addition, the bromination increases the resin density which allows for settling in fermentation broths, and for usage in upflow fluidized beds. SP207SS is a small size version of modified aromatic type Sepabeads SP207. SP207SS is used for applied to reversed phase chromatography. The brominated polymeric matrix provides unique selectivity, full pH operating range and long operating life versus conventional bonded silica packing materials used in preparative and industrial applications. HP2MG is a macroporous methacrylate polymeric resin. It has no aromatic character. HP2MG grades have different selectivity than the classic Diaion HP20. HP20 is recommended for broad spectrum adsorption and desorption of small and large molecules, long chain aliphatic molecules, de-colorization and desalting applications, and should be evaluated as an alternate resin matrix to the styrenic based adsorbents. SP2MGS is a small and uniform particle size version of the methacrylate type Diaion HP2MG. SP2MGS gives higher dynamic capacity than HP2MG and shows strong retention and unique selectivity in normal phase chromatography.
22
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Product
HP20 SP825L SP70 SP700 SP710 SP207 HP2MG HP20SS
Matrix Type
Highly Porous (styrene, DVB) Highly Porous (styrene, DVB) Highly Porous (styrene, DVB) Highly Porous (styrene, DVB) Highly Porous (styrene, DVB) (Brominated styrene-DVB) (Methacrylate) Highly Porous (styrene, DVB)
Ceph C Capacity* (g/L)

~38 ~80 ~120 <10 -
Water Retention (%)

55-65 52-62 55-65 60-70 60-70 43-53 55-65 55-67
Shipping Density (g/L)

~680 ~685 ~685 ~680 ~685 ~790 ~720 ~670
Particle Sze Distribution

On 250 m: 90% min On 250 m: 90% min On 250 m: 90% min On 250 m: 90% min On 250 m: 90% min On 250 m: 90% min On 300 m: 90% min >150 m: 15% max 63-50 m: 70% min <63 m: 20% max >75 m: 30% max 63-75 m: 55% min <63 m: 15% max >150 m: 15% max 63-150 m: 70% min <63 m: 20% max >220 m: 1% max 120-160m: 85% min <90 m: 1% max
SP20SS
Highly Porous (styrene, DVB)
55-65
~660
SP2O7SS
(Brominated styrene-DVB)
43-53
~780
SP2MGS
(Methacrylate)
61-69
~720
Product
HP20 SP825L SP70 SP700 SP710 SP207 HP2MG HP20SS SP20SS SP2O7SS SP2MGS
Effective Size (mm)

0.25 min 0.25 min 0.25 min 0.25 min 0.25 min 0.25 min 0.30 min -
Surface Area (m2/dry-g)

~590 ~930 ~870 ~1200 ~900 ~600 ~570 ~560 ~560 ~590 ~520
Pore Volume (ml/gram)

~1.3 ~1.4 ~1.5 ~2.3 ~1.0 ~1.3 ~1.2 ~1.2 ~1.0 ~1.3
Pore Radius ()
290 70 70 90 90 110 240 ~290 ~290 ~110 ~230
Specific Gravity
1.01 1.01 1.01 1.01 1.01 1.19 1.09 -
Swelling Water to Toluene

30% 20% 24% 7 20 5 -
Operation Tempuratures
130 C max 130 C max 130 C max 130 C max 130 C max 130 C max 130 C max 130 C max 130 C max 130 C max -
Purification of Antibiotics
Non-ionic synthetic adsorbents are able to hydrophobically adsorb antibiotics which do not have dissociating groups. In addition, they are also able to adsorb those with carboxyl groups, because under the weakly acid pH conditions in which antibiotics are stable, the carboxyl group is hardly dissociated. Moreover, their adsorption is usually strongest under weakly acid conditions and, therefore these adsorbents find wide application. The figure to the right shows the elution behavior of cephalosporin C. While operating conditions will not be exactly identical, there are many cases where a substance which can be separated by C18 Silica Gel may also be separated by the use of a synthetic adsorbent, such as Diaion HP20 in this case.
Adsorption conditions Influent Solution: 4300 ppm C.P. SV: 4 BTP: 2150 ppm Rinsing Volume: 2BV/HP20 Elution Condition: SV 4 Adsorbent: 20 ml (Diaion HP20) 30% Aqueous Isopropanol Solution
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23
Styrenic & Methacrylic Reversed Phase Resins for Preparative Chromatography Styrenic Type: MCI Gel CHP20P/P20, CHP20/P30, CHP20/P50 dry Brominated Type: MCI Gel CHP07/P10 Methacrylate Type: MCI Gel CMG20/P10, CMG20/P30
The CHP grade resins are based on a highly crosslinked polystyrene matrix with no functionality. These resins offer varying pore size, surface area, and bead size to allow the chromatographer to control the efficiency and the resolution in the separation of pharmaceuticals (e.g. heterocyclic compounds), steroids, small peptides and proteins, oligonucleotides, amphoteric molecules (e.g. sulfonamides, antibiotics, nucleic bases), basic drugs (e.g. anticonvulsants), simple amines (e.g. catecholamine), and antihistamines, etc. These styrenic matrices have excellent mechanical strength with wide range pH stability (1 to 14) and resistance to high temperatures (up to 120 C operating temperature). CHP20 grades are the preparative size for HP20. CHP07 grades are the preparative size for SP207. The CMG grade resins are based on highly crosslinked polymethacrylic matrix with no functionality. Polymethacrylate resins have no aromatic character, offering medium hydrophobicity (less hydrophobic than styrenic resins or octadecyl silane). They are mainly used in the separation of pharmaceuticals (e.g. polyaromatic and polyaliphatic compounds), water soluble vitamins, small peptides and proteins, oligonucleotides, simple amines (e.g. catecholamine), and agricultural chemicals, etc. These methacrylic matrices have excellent mechanical strength and wide range pH stability (1 to 13), and resistance to high temperatures (up to 120 C operating temperature). CMG grades are the preparative size for HP2MG
Product
CHP20P/P20 CHP20P/P30 CHP20/P50 dry CHP07/P10
Matrix
Styrene-DVB Styrene-DVB Styrene-DVB Bromintated Styrene-DVB
Mean Size
20 m 30 m 50 m 10 m
Particle Range
18-22 m 20-40 m 37-75 m 9-11 m
Pore Diameter (Ang)

450 450 450 250
Column Dimension
None None None 4.6 x 150 mm 10 x 250 mm 20 x 150 mm 20 x 250 mm 4.6 x 150 mm 10 x 250 mm 20 x 150 mm 20 x 250 mm None
Packing Sizes
25, 100, 1000 ml 25, 100, 1000 ml 100 gram 500 ml, 40 L
CMG20/P10
Methacrylate
10 m
9-11 m
250
10 g, 1000 g
CMG20/P30
Methacrylate
30 m
25-33 m
250
25, 100, 1000 ml
Bioseparation: Wide Pore Hydrophilic Cation Exchange Resins SP (Sulfopropyl) Type: MCI Gel CQK30S, CQK30P CM (Carboxymethyl)Type: MCI Gel CQK31S, CQK31P
The CQ grade resins are based on highly crosslinked polymethacrylic matrix, which is further hydroxylated for added hydrophilicity. These matrices are functionalized with either sulfonic acid or carboxymethyl to form strong acid and weak acid cation exchangers respectively with a sodium counterion. By varying the bead size of these resins, the chromatographer can control the efficiency and the resolution in the separation of cationic proteins, peptides, and polynucleotides (separation at a pH < PI of the target molecule). These methacrylic polymer matrices have excellent mechanical strength with wide range pH stability (1 to 13) and resistance to high temperatures (can be autoclaved at 121 C for 20 minutes).
Product
Mean Size
10 m 30 m 10 m 30 m
Particle Range
9-11 m 25-35 m 9-11 m 25-35 m
Funct. Group
SP SP CM CM
Counter Ion
Na+ Na+ Na+ Na+
Funtional Degree
0.3-0.5 meq/g 0.3-0.5 meq/g 0.2-0.4 meq/g 0.2-0.4 meq/g
Exclusion Limit
Up to 106 Up to 106 Up to 106 Up to 106
Pore Diam. (Ang)

600 600 600 600
Column Dimension
75 x75 mm None 75 x 75 mm
Packing Sizes *
CQK30S CQK30P CQK31S CQK31P
10, 25, 50 g 100 ml 10, 25, 50 g 100 ml
24
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Bioseparation: Wide Pore Hydrophilic Anion Exchange Resins QA Type: MCI Gel CQA35S, CQA35P DEAE Type: MCI Gel CQA31S, CQA31P Diethyl Amine Type: Sepabeads FP-DA13
The CQ grade resins are based on a highly crosslinked polymethacrylic matrix, which is further hydroxylated for added hydrophilicity. These matrices are functionalized with either a quaternary amine (trimethyl amine) or a diethyl-aminoethyl to form strong basic and weak basic anion exchangers respectively with a chloride counterion. By varying the bead size of these resins, the chromatographer can control the efficiency and the resolution in the separation of anionic proteins, peptides, and polynucleotides (separation ran at a pH > PI of the target molecule). These methacrylic matrices have excellent mechanical strength with wide range pH stability (1 to 13) and resistance to high temperatures (up to 60C operating temperature). The FP grade resin is based on a highly crosslinked polymethacrylic matrix, which is hydroxylated for added hydrophilicity. This matrix is functionalized with a diethyl-amino to form weak basic anion exchanger. This resin is used in the large scale separation of anionic proteins, peptides, and polynucleotides (separation ran at a pH > PI of the target molecule) as well as enzyme immobilization supports. This methacrylic matrix has excellent mechanical strength with wide range pH stability (1 to 13) and resistance to high temperatures (up to 60C operating temperature). Unique around ion-exchange resins. The diethylamine (DEA) functionality is a secondary amine base group which provides increased capacity towards target molecules.
Prod
Mean Size
10 m 30 m 10 m 30 m 120 m
Particle Range
9-11 m 25-35 m 9-11 m 25-35 m 100-150 m
Funct. Group
QA QA DEAE DEAE Diethyl amine
Counter Ion
ClClFree Base Free Base Free Base
Funct. Deg. Exclusion Limit

0.3-0.5 meq/g Up to 106 0.3-0.5 meq/g Up to 106 0.5-0.7 meq/g Up to 106 0.5-0.7 meq/g Up to 106 1.9 meq/ml Up to 106 -
Capacity BSA
Pore Diamtr. (Ang)

600 600 600 600 600
Column Dim.
75 x 75 mm None 75 x 75 mm None None
Packing Sizes
10, 25, 50 g 25, 50, 100, 1000 ml 10, 25, 50 g 25, 50, 100, 1000 ml 500 ml, 40 L
CQA35S CQA35P CQA31S CQA31P FP-DA13
15-20 mg/ml
Strong Acidic Cation Exchange Resins Gel Type: Diaion SK1B, UBK10 Porous Type: Diaion PK228, RCP160M
Strong Acidic Cation Exchange Resins are cross-linked polystyrene resins having sulfonic acid groups. Among the Diaion Product line, there are SK grades of gel type, PK grades of porous type, RCP160M of highly porous type. The standard shipping is sodium salt form . In some cases, the hydrogen form is available for catalysis applications such as esterification, alkylation, hydrolysis, hydration, etc. SK1B in Na+ and H+ form resin is our flagship premium grade, gel-type, strong acid cation resin. It has excellent properties for industrial applications and is recommended for industrial scale softening and de-mineralization applications. Featuring standard crosslinkage as is used in situations where the raw water contains oxidizing substances. The PK resin grades are based on a porous styrene DVB polymer matrix. Their porous structure provides excellent durability against osmotic shock by swelling and shrinkage. PK228 is recommended for condensate de-mineralization (L grade is recommended for best pressure flow performance). PK grades area also recommended for special applications such as deashing, de-colorization, and as a catalyst (as they have higher reaction rate in organic solvents than gel type resins). Unlike other ion-exchange resins, the UBK ion-exchange resin featuring good uniformity in particle size, reducing the reagent costs and spent waste waters useful where high purity water is needed for manufacturing processes. UBK ion-exchange resins are also used in many other fields because they have few large particle which can be easily broken by shrinkage and swelling. Diaion RCP160M is based on a macroporous crosslinked polystyrene matrix with sulfonic acid functional groups. This matrix is more open and porous than conventional grade resin with increased polymer surface available for intimate contact with solutes. In catalysis, the reaction rates are faster with RCP160M than standard grade resins, resulting in a higher degree of conversion during catalytic applications.
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25
Product
SK1B* PK228* UBK10 RCP160M
Matrix
Gel (styrene, DVB): R-SO3-Na+ Porous (styrene, DVB): R-SO3- Na+ Gel (styrene, DVB): R-SO3- Na+ Highly Porous styrene, DVB R-SO3- H+
Counter Ion
Na+ for standard form, H+ for catalyst applications and de-mineralization Na+ for standard form, H+ for catalyst applications and de-mineralization Na+ for standard form, H+ for catalyst applications and de-mineralization Na+ for standard form, H+ for catalyst applications and de-mineralization
Total Capacity (meq/ml-r)

>2.0 Na form >1.7 H form >2.05 Na form >1.9 H form > 2.2 Na form >1.5 H form
Water Rentention (%)

4350 Na form 50-60 H form 37-43 Na form 39-49 H form 35-45 Na form 45-55 H form

~ 825 Na form ~ 780 H form ~ 805 Na form ~ 770 H form ~ 850 Na form -
Product
SK1B* PK228* UBK10 RCP160M
Particle Size Distribution

On 1180 m: 5% max Thru 300 m: 1% max On 1180 m: 5% max Thru 300 m: 1% max 580 20 m On 710 m: 25% max Thru 250 m: 1% max
Effective Size (mm)

0.40 min 0.40 min -
Uniformity Coefficient
1.6 max 1.6 max 1.2 max 1.6 max
Whole Bead Count (%)

90% min 95% min 90% min 95% min
Operating Temp.
120C (H form, Na form) max 120C (H form, Na form) max 120C (H form, Na form) max 120C (H form, Na form) max
Crosslinked
8% 14% 10% High
Strong Basic Anion Exchange Resins Gel Type: Diaion SA10 (Type I), SA20A (Type II) Porous Type: Diaion PA308 (Type I), PA418 (Type II), HPA25L (Type I)
Diaion Strong base anion exchange resins are based on a cross-linked polystyrene matrix having quaternary ammonium groups (NR3+). Among the Diaion product line, there are SA-grades of gel type, PA-grades of porous type, and HPA-grades of highly porous-type. The standard shipping is Cl- form (in some cases OH- form is available). Type I resins have trimethyl ammonium groups which impart higher basicity. In the co-current regeneration mode, the treated water will have the lowest silica leakage. Type I resins also have higher chemical stability and can be applied at higher temperature than Type II or acrylic based resins. SA10A is a Type I resin with standard crosslinkage. Porous-type ion exchange resins of a porous polymer matrix have good resistance against swelling and shrinking, though their exchange capacity is lower than gel-type ion exchange resins of the same degree of crosslinkage. These are effective when highly purified effluents are needed, e.g. removal of silica to very low concentration. They are suitable for treatments of waste waters with organic compounds. PA308 is used for de-mineralization and de-colorization of sugar liquors.
Product
Matrix
Counter Ion
Total Capacity (Cl- form) (meq/ml-R)

> 1.3 > 1.0 > 0.5 >1.3 >1.3
Water Rentention (%) (Cl- form)

43-47 57-67 58-68 45-52 38-44
Shipping Density (g/L) (Cl- form)

~ 670 ~ 710 ~ 680 ~ 715 ~ 685
SA10A PA308 HPA25 SA20A PA418
Gel (styrene, DVB): R-CH2- N(CH3)3+ Porous (styrene, DVB): R-CH2- N(CH3)3+ Highly Porous Gel (styrene, DVB) Porous (styrene, DVB)
Cl- for standard form, OH- for catalyst applications and de-mineralization Cl- for standard form, OH- for catalyst applications and de-mineralization Na+ for standard form, H+ for catalyst applications and de-mineralization -
26
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Product
Functionallity

On 1180 m: 5% max Thru 300 m: 1% max On 1180 m: 5% max Thru 300 m: 1% max On 250 m: 5% min On 1180 m: 5% max Thru 300 m: 1% max On 1180 m: 5% max Thru 300 m: 1% max
Effective Size (mm)

0.40 min 0.40 min 0.25 min 0.40 min 0.40 min
1.6 max 1.6 max 1.6 max 1.6 max 1.6 max

90% min 95% min 95% min 90% min 95% min
Operating Temp.
60C (OH form) 80C (Cl form) max 60C (OH form) 80C (Cl form) max 60C (OH form) 80C (Cl form) max 40C (OH form) 60C (Cl form) max 40C (OH form) 60C (Cl form) max
Crosslinked
4% High 9%
SA10A PA308 HPA25 SA20A PA418
R-CH2- N(CH3)2(CH3CH2OH) + Cl- for standard form R-CH2- N(CH3)2(CH3CH2OH) + Cl- for standard form
Weakly Acidic Cation Exchange Resins Methacrylic Type: DiaionWK100 Acrylic Type: Diaion WK60L
WK100 (methacrylic type) is a weak acid cation exchange resins having carboxylic acid functionalities. It has a pK value of approximately 6. Used mainly used for the purification of pharmaceuticals and foods. WK60L (acrylic type) is a weak acid cation exchange resin with carboxylic acid functionality based on porous acrylic polymer matrix. They have a pK value of approximately 5.3. The acrylic type resins have higher total capacity than methacrylic type resins. They are mainly used for water treatment applications such as the removal of hardness ions in the presence of bicarbonate alkalinity.
Product
WK100 WK60L
Matrix
Porous - Methacrylic Acrylic
Total Capacity Water Rentention (meq/ml-R) (%)

>2.8 4.4 (min.) 45-55 44-52
Functionality
Carboxylic Acid Carboxylic Acid

On 1180 m: 5% max Thru 300 m: 1% max On 1180 m 10% (max) Thru 425 m 3%
Effiective Size (mm)

0.40 min 0.40 min
Product
WK100 WK60L
Uniformity Coeffectient
1.6 max 1.6 max

~ 660 ~ 800

95% min 95% min
Operating Temperature
150C max 120C (max) (Na and H form)
Effective pH Range
5 - 14 4-14
Weakly Basic Anion Exchange Resins Acrylic/Amide Type: Diaion WA10 Styrenic/Polyamine Type: Diaion WA20 Styrenic/Dimethylamine Type: Diaion WA30 Styrenic/Adsorbent Type: Diaion DCA11
WA10 resin is based on a gel-type acrylic polymer matrix. It has a tertiary amine functionality with a high regeneration efficiency. The acrylic polymer matrix provides good chemical stability and good resistance to organic fouling. WA10 is mainly used for pretreatment of starch hydrolysates, beet sugar solutions, and formaldehyde. WA20 has high total exchange capacity and high regeneration efficiency. The porous styrene polymer imports high chemical stability, high mechanical strength against attrition loss, high thermal stability, and high durability against organic fouling. These resins are used for the removal of strong mineral acids in standard water treatment applications. It can also be applied in special process separations such as the treatment of organic solvents. It does not have any neutral salt splitting capacity. WA30 resin is a weak base anion exchange resin based a on porous, styrene-DVB polymer matrix with dimethyl amine functionality. The high porous styrenic matrix has excellent chemical stability, mechanical and osmotic strength, high thermal stability, and durability against organic fouling. WA30 is the benchmark to which all macroporous weak base exchange resins are compared to. It is used for a variety of applications, such as removal of organic substances of high molecular weight, pretreatment of raw waters containing organic foulants, de-ionization and de-colorization of starch hydrolysates, and purification of glycerine and enzymes.
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DCA11 is a lightly functionalized adsorbent resin based on a crosslinked styrene/DVB polymer matrix. DCA11 has a highly developed macroporous polymer structure with a narrow pore size distribution. The matrix is lightly functionalized with dimethylamino groups to provide some weakly basic anion exchange capacity. The styrene/DVB matrix offers hydrophobic interaction mechanisms and the amino group provides some ion exchange selectivity for improved de-colorization performance. DCA11 is recommended for de-colorization of natural organic aqueous solutions, such as fermentation liquids.
Product
Matrix
Functionality
Total Capacity (Free base form) (meq/ml-R)

> 1.2 > 2.5 > 1.5 > 0.3

63-69 41-47 43-55 50-60

~ 695 ~ 660 ~ 635 ~ 630
WA10 WA20 WA30 DCA11
Gel-Acyrlic Porous-Styrene DVB Porous-Styrene DVB Highly Porous-Styrene DVB
-CONH(CH2)nN(CH3)2 -CH2NH(CH2CH2NH)nH -CH2N(CH3)2 -CH2N(CH3)2
Product
WA10 WA20 WA30 DCA11

On 1180 m: 5% max Thru 300 m: 1% max On 1180 m: 5% max Thru 300 m: 1% max On 1180 m: 5% max Thru 300 m: 1% max On 1180 m: 5% max Thru 300 m: 1% max
Effective Size (mm)

0.35 min 0.40 min 0.40 min 0.40 min
1.6 max 1.6 max 1.6 max 1.6 max

90% min 95% min 95% min 95% min
Operating Tempurature
60C max 100C max 100C max 100C max
Effective pH range
0-9 0-9 0-9 -
Specifc Surface Area

600m2/dry gram
Chelating Resins Iminodiacetate Type: Diaion CR11 Polyamine Type: Diaion CR20 Glucamine Type: Diaion CRB03 and CRB05
CR11 is based on a high porous styrenic matrix, and provides rapid kinetics, high operating capacity, low swell / shrink ratio, and excellent mechanical stability. CR11 captures metal ions by chelation with its iminodiacetate functionality. CR11 has a higher selectivity than strong and weak acid cation exchange resins for divalent ions, especially transitional metal elements such as copper, iron, etc. CR11 can capture metal ions at much lower pH than strong and weak acid resins. As CR11 has higher selectivity for divalent metal ions than monovalents, it can be used for such selective separation. CR20 is a special chelating resin with polyamine functionality. CR20 captures metal ions by chelation with its polyamine functionality with its high porous styrene-DVB matrix. CR20 has a high selectivity to heavy metal ions. It does not adsorb alkali metal and alkali earth metal ions, and therefore, it can be used for heavy metal recovery from highly concentrated alkali earth solutions. CRB03 and CRB05 are special chelating resin with glucamine groups based on a high porous styrene-DVB matrix. It has high selectivity for borate ions. It is used for borate separation from various solutions, including brine and sea water.
Product
Matrix
Functionality
Total Exchange Capacity (meq/ml-R)

> 0.7 > 0.95
Cu Adsorption Capacity (mmole/ml-R)

>0.5 >0.4 -

55-65 60-60 45-55 43-53
CR11 CR20 CRB03 CRB05
Porous-Styrene DVB Porous-Styrene DVB Porous-Styrene DVB Porous-Styrene DVB
-CH2N(CH2COONa)2 -CH2NH(CH2CH2NH)nH -CH2N(CH3)CH2(CHOH)4CH2OH -CH2N(CH3)CH2(CHOH)4CH2OH
Product
CR11 CR20 CRB03 CRB05

~ 730 ~ 635 ~ 665 ~ 770

On 1180 m: 5% max Thru 355 m: 2% max On 1180 m: 5% max Thru 300 m: 1% max On 1180 m: 5% max Thru 300 m: 1% max On 850 m: 10% max Thru 300 m: 1% max
Effective Size (mm)

0.40 min 0.40 min 0.35-0.55 min 0.35 min
1.6 max 1.6 max 1.6 max 1.6 max

95% min 95% min 95% min 95% min
80C max (H form) 120C max (Na form) 100C max 100C max 100C max
28
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Ultrapure Mixed Bed Resins Gel Type: Diaion SMT100L, SMT200L

Mitsubishi Chemical offers several grades of mixed resins for non-regenerable mixed bed ion exchange applications. All mixed resins are typically offered in a 1:1 stoichiometric ratio (1 equivalence of cation equilibrium capacity to 1 equivalence of anion equilibrium capacity). SMT100L and SMT200L are manufactured to very stringent performance specifications, and prepared from component resins with the highest degree of conversion and considerations of purity. They will also provide exceptional performance relative to electrical resistivity and TOC leakage. In fact, rinsing to 18 egaohm resistivity is nearly instantaneous, and clearly superior to conventional mixed bed resins. The performance for TOC meets or exceeds all conventional mixed bed resins. The TOC rinse down performance is maintained over 12 months of storage. Diaion SMT200L offers remarkable performance relative to metals leakage. The cation component, SKT20L, has been prepared with low levels of metals in the resin phase (500 ppb). This improvement allows ppt metals leakage in the product water quality (actual performance is <0.1 ppt for 13 metals within 4 hrs. of rinse). These are mixed bed resins: 1 equivalent of cation exchanger to 1 equivalent of anion exchanger. This mixed bed is used for the production of ultrapure water for use in the semiconductor industry as well as in the production of high purity chemicals. The SMT200L has a higher purity than SMT100L as the ash content is reduced several folds within the resin matrix.
Product
SMT100L
Matrix
Gel (styrene, DVB): with Ionic form H/OH Gel (styrene, DVB): with Ionic form H/OH
Capacity (meq/ml-R)
> 1.7 cation > 0.9 anion > 1.7 cation > 0.9 anion

50-60 cation 62-72 anion 50-60 cation 62-72 anion
Conversion (eq%)
>99.9H form >90.0 OH form <1.0 Cl form >99.9H form >90.0 OH form <1.0 Cl form

On 1180 m: 5% max Thru 300 m: 1% max On 1180 m: 5% max Thru 300 m: 1% max
Effiective Size (mm)

0.40 mm
SMT200L
0.40 mm
Product
SMT100L SMT200L
Uniformity Coeffectient
1.6 max 1.6 max -
Performance Properties*
Resistivity (m . Cm)
>18 (3 hrs) >18.1 (12 hrs)
TOC (ppb)
<10 (3 hrs_ <10 (3 hrs) -
Metal Water Qualities (ppt)

<0.1 (12 hrs)
Particles (>0.05 m/ml)

<10 (12 hrs)
Fractionation Resins for Industrial Chromatography Gel Type: Diaion UBK550, UBK555
Chromatographical separation is widely industrialized in pharmaceuticals, fermentation, and food applications. Ion exchange resins of small particles with uniform particle size distribution are applied for these purposes. The smaller particles with the more uniform particle size distribution, the higher yields and purities can be obtained. These types of resins are suitable for industrial chromatography such as fructose/glucose separation. UBK resins are prepared via a monodispersed production technology, and offered with narrow particle size distributions in either sodium or calcium forms. The high bead uniformity provides fractionation resins with excellent resolving power and mechanical stability. These products are often applied in simulated moving beds (SMB) equipment with a 4 to 8 column design for separation of binary and ternary mixtures in both small and large scale industrial applications.
Product
UBK550 UBK555
Matrix
Gel (styrene, DVB): R-SO3Gel (styrene, DVB): R-SO3-
Counter Ion
NA+ form CA+ form
Total Capacity (meq/ml-R)

> 1.9 > 2.0

46-50 42-46

~ 825 Na form ~ 865
Product
UBK550
Particle Size
Standard -220 m K grade - 350 m G grade - 390 m Standard -220 m K grade - 350 m
1.1 max

90% min
120C max 8%
Crosslinked
UBK555
1.1 max
90% min
120C max
8%
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29
SPE Cartridges
Chromabond Solid Phase Extraction
Solid Phase Extraction (SPE) is a fast and efficient sample preparation technique for cleanup or concentration analysis, HPLC, GC, TLC, UV, IR and is an excellent tool for method development. SPE has capabilities for a wide spectrum of applications including environmental analyses, pharmaceutical and biochemical analyses, organic chemistry and food analysis.
Advantages of Solid Phase Extraction:

Lower consumption of solvents Faster-enormous time savings Less demand placed on analytical equipment Lower costs per sample Potential for automation High consistency in individual sample handling More specific selectivity because of the broad range of adsorbents and different retention mechanisms Optimization of extraction by variation or adjusting of the solid phase and chromatographic conditions
Ultra-high performance Chromabond SPE Cartridges are available in polypropylene (standard & Large Volume) and glass tubes from 1 to 70ml sizes. Chromabond SPE Cartridges are precision packed with a wide array of sorbents: SiOH, Alumina (A, B, N), C18, C8, C4, C2, Cyclohexyl, Phenyl, NitroPhenyl, Amino, DMA, CN, Diol, PCA, SA, PSA, SB, PS-RP, PS-OH, PS-H+, PS-Ag+, PS-Ba2+ , Florisil, Polyamide, Phases for Environmental, and Specialty. Phases are also packed in Chromafix (luer connect) and 96 Well Plates. Ask us about Method Development Kits containing a mixed selection of sorbents and Bulk Packs. Vacuum Manifolds and SPE accessories available.
How to find the right ChromaBond phase

Selection guide for SPE phases and solvents
30
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Main steps of the SPE procedure

1. Conditioning of the adsorbent
Conditioning of the adsorbent is necessary in order to ensure reproducible interaction with the analyte. Conditioning, also called solvation, results in a wetting of the adsorbent and thus produces an environment which is suitable for adsorption of the analyte. Nonpolar adsorbents are usually conditioned with 2 3 column volumes of a solvent, which is miscible with water (methanol, THF, -propanol etc.), followed by the solvent in which the analyte is dissolved (pure matrix, e. g. water, buffer). Polar adsorbents are conditioned with nonpolar solvents. After the conditioning step, the adsorbent bed must not run dry to maintain solvation (deconditioning).
2. Sample application (adsorption)

Sample application can be performed with positive or negative pressure with a flow rate of ~3 ml/min. Sample volumes vary from a few milliters up to liters.
3. Washing of the adsorbent

Washing of the adsorbent is usually achieved with a special wash solution; however, in some cases it may not be necessary. If the polarity difference between the wash solution and the eluent is very large, or if both are not miscible, drying of the adsorbent bed after washing is recommended to improve elution and recovery.
4. Elution
Elution with a suitable eluent should not be too fast. The elution speed depends on the column or cartridge dimensions and the quantity of adsorbent (about 1 ml/min).
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SPE Cartridges - Chromabond

Benefits of Chromabond
High recovery rates Excellent reproducibility Improves selectivity of the analysis Reduces consumption of organic solvents Speeds analysis time Provides variety and selection for even the most specific application Over 40 Phases available All SPE products sealed to maintain quality and shelf life Cartridges available in polypropylene, glass LV (large volume), 96 well plates
Silica Gel
Based normal phases for SPE
Properties:
Unmodified, weakly acidic silica, pore size 60, particle size 45m, specific surface 500m2/g, pH stability 2 8 Very polar Adsorbs humidity from air, for this reason it should be kept well closed and if necessary dried before use Due to its high affinity for polar compounds it should not be conditioned with polar (e.g. methanol) or water-containing solvents
Recommended Applications:
Aatoxins Chloramphenicol Pesticides Steroids Vitamins
Cat. #
730070 730075 730217 730072 730214G 730075G Silica Gel Silica Gel Silica Gel Silica Gel
Product
500mg 1g 2g 10g 200mg 1g
Weight
6ml 6ml 15ml 70ml 3ml 6ml
Size
30 30 20 10 50 30
Qty/pkg
Silica Gel - Glass Silica Gel - Glass
For more selections and sizes, visit our website or contact your personal Product Specialist
C18 ec
Properties:
Base material silica, pore size 60, particle size 45m for C18, 100m for C18, pH stability 2 8 Octadecyl phases, not end-capped, carbon content 14% Very nonpolar, hydrophobic interactions with a wide variety of organic compounds Advantageous for clean-up of samples with large structural variations (polarity differences)
Nonpolar compounds Aatoxins, amphetamines, antibiotics, Anti-epileptics, barbiturates, caeine, drugs, preservatives, fatty acids, nicotine, PAHs, pesticides, PCBs, heavy metals, vitamins Very well suited for desalting of samples
Anti-epileptics from serum

Cartridge: CHROMABOND; C18 ec / 1 ml / 100 mg Phase: CHROMABOND C18 ec Catalog #: 730011 Substances: carbamazepine phenobarbital phenytoin primidone Information: Mix 500 l serum with 500 l internal standard (500 g / ml 4-methylprimidone in citrate buffer pH Conditions: Column conditioning: 2 column volumes methanol, 2 column volumes water Sample application: Slowly force or aspirate the sample through the column Column washing: 2 column volumes dist. water.
Cat. #
730011 730012 730013 730014 730015 730404 730405 730259 730012 G 730014 G 732012 732013
Product
C18 ec C18 ec C18 ec C18 ec C18 ec C18 ec C18 ec C18 ec C18 ec - Glass C18 ec - Glass C18 ec - Lg. Vol. C18 ec - Lg. Vol.
Weight
100mg 200mg 500mg 500mg 1g 2g 5g 10g 200mg 500mg 200mg 500mg
Size
1ml 3ml 3ml 6ml 6ml 15ml 45ml 70ml 3ml 6ml 15ml 15ml
Qty/pkg
100 50 50 30 30 20 20 10 50 30 30 30
32
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C8
Properties:
Base material silica, pore size 60, particle size 45m, specific surface 500 m2/g, pH stability 2 8 Octadecyl phases, not end-capped, carbon content 8% Similar to C18, however slightly more polar Secondary interactions with polar compounds are more pronounced due to shorter alkyl chains
Pesticides, PCBs
Cat. #
730021 730022 730023 730024 730134 730024G 732023 C8 C8 C8 C8 C8 C8 - Glass
Product
100mg 200mg 500mg 500mg 1000mg 500mg 500mg
Weight
1ml 3ml 3ml 6ml 6ml 6ml 15ml
Size
100 50 50 30 30 30 30
Qty/pkg
C8 - Large Volume
C4
Properties:
Base material silica, pore size 60, particle size 45m, specific surface 500 m2/g, pH stability 2 8 Butyl phase, not end-capped, carbon content 7% Slightly more polar than C18 or C8, due to shorter alkyl chains the silica surface is not completely shielded
Compounds, which are too strongly retained on C18 or C8 e.g. analgetics from blood
Cat. #
730225 730227 C4 C4
Product
100mg 500mg
Weight
1ml 3ml
Size
100 50
Qty/pkg
C2
Properties:
Base material silica, pore size 60, particle size 45m for C18, 100 m for C18, pH stability 2 8 Dimethyl phase, not end-capped, carbon content 4 % Similar to C4
E.g. anti-epileptics from plasma
Cat. #
730169 730221 73409 730410 C2 C2 C2 C2
Product
100mg 500mg 500mg 1g
Weight
1ml 3ml 6ml 6ml
Size
100 50 30 30
Qty/pkg
For more selections and sizes, visit our website or contact your personal Product Specialist Sorbent Technologies, Inc. Toll Free: 866.SORBTEC Fax: 770.936.0326 www.sorbtech.com 33
Phenyl
Properties:
Base material silica, pore size 60 , particle size 45 m for C18, 100 m for C18, pH stability 2 8 Phenyl phase, carbon content 8% Polarity similar to C8 In addition to hydrophobic interactions more selective adsorption is possible by - interactions due to the electron density of the phenyl ring
Aatoxins Caeine Phenols
Cat. #
730083 730411 730084 Phenyl Phenyl Phenyl
Product
100mg 200mg 500mg
Weight
1ml 3ml 3ml
Size
100 50 50
Qty/pkg
Amino (NH2)
Properties:
Base material silica, pore size 60 , particle size 45 m for C18, 100 m for C18, pH stability 2 8 Aminopropyl phase, carbon content 3.5% Polar, weak anion exchange
Trace elements Lipids
Cat. #
730031 730413 730033 730180 730626 730033G 730180G 730626G 732033 Amino (NH2) Amino (NH2) Amino (NH2) Amino (NH2) Amino (NH2)
Product
100mg 200mg 500mg 500mg 1g 500mg 500mg 1g 500mg
Weight
1ml 3ml 3ml 6ml 6ml 3ml 6ml 6ml 15ml
Size
100 50 50 30 30 50 30 30 30
Qty./Pkg.
Amino (NH2) - Glass Amino (NH2) - Glass Amino (NH2) - Glass Amino (NH2) - Large Volume
Alumina
Properties:
Aluminium oxide, high purity, pore volume 0.90 ml/g, particle size 60 150 m, specific surface 150 m2/g
Together with phase SA for PCB and pesticides
Cat. #
730452 730429 730446 730017 730139 730467 730139G 730020G 732210 732091 732205 Alumina Acid Alumina Basic Alumina Neutral Alumina Acid Alumina Neutral Alumina Basic
Product
500mg 500mg 500mg 1g 1g 4g
Weight
3ml 3ml 3ml 6ml 6ml 45ml
Size
50 50 50 30 30 20
Qty/pkg
Alumina Neutral - Glass Alumina Basic - Glass Alumina Acid - Large Volume Alumina Neutral - Large Volume Alumina Basic - Large Volume
1g
1g 1g 1g 1g
6ml
6ml 15ml 15ml 15ml
30
30 30 30 30
34
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Diol
Properties:
Base material silica, pore size 60 , particle size 45 m for C18, 100 m for C18, pH stability 2 8 Diol phase, carbon content 5.5% Polar Properties similar to SiOH
Antibiotics Prostaglandins
Cat. #
730051 730417 730053 730418 730053G 732053 Diol Diol Diol Diol
Product
Weight
100mg 200mg 500mg 500mg 500mg 500mg 1ml 3ml 3ml 6ml 3ml
Size
Qty/pkg
100 50 50 30 50 30
Diol - Glass Diol - Lg. Vol.
15ml
Also available in Glass!
Cyano
Properties:
Base material silica, pore size 60 , particle size 45 m for C18, 100 m for C18, pH stability 2 8 Cyanopropyl phase, carbon content 5.5% Polar to mid-polar In addition to weak hydrophobic interactions, selective interactions are possible due to the high electron density of the Cyano group
Cyclosporins Carbohydrates
Cat. #
730061 730420 730063 730421 Cyano Cyano Cyano Cyano
Product
100mg 200mg 500mg 500mg
Weight
1ml 3ml 3ml 6ml
Size
100 50 50 30
Qty/pkg
Florisil
Properties:
Matrix magnesium silicate (MgO - SiOH 15:85) High purity, Particle size 150 250 m
Organic tin compounds, aliphatic carboxylic acids, PCBs, PAHs
Cat. #
730457 730081 730238 730082 730082G Florisil Florisil Florisil Florisil Florisil - Glass
Product
200mg 500mg 500mg 1g 1g
Weight
3ml 3ml 6ml 6ml 6ml
Size
50 50 30 30 30
Qty/pkg
Easy
Polar, bifunctionally modified polystyrene-divinylbenzene copolymer
Because of the bifunctional modification Chromabond Easy is significantly more hydrophilic than conventional polystyrene-divinylbenzene polymers and is therefore easily wettable with water. Aqueous sample can be loaded directly with little or no preconditioning. There is no decrease in recovery rates if the cartridge runs dry and therefore automation is easier because a permanent vacuum can be used without supervision.
Properties:
Weak anion exchanger Specific surface area 650 - 700m2/g, Particle size 80m, pore size 50 pH stability 1 - 14 More hydrophilic than conventional polystyrene-divinylbenzene Easily wettable with water
Polar herbicides / pesticides from water (acidic, neutral, basic) Polar phenols from water Polyaromatic compounds Polychlorinated biphenyls Drug analysis from urine, blood, serum, plasma Pharmaceuticals / active ingredients from tablets, creams
Recovery rates of Phenyl urea pesticides

Cartridge: CHROMABOND; EASY 3ml 200mg Phase: CHROMABOND Easy Catalog #: 730754 Substances:
fluometuron fenuron monuron isoproturon chlortoluron linuron diuron metoxuron metobromuron monolinuron
Information:
Drinking water; internal standard 100 ng/l of each substance
Conditions:
Conditioning: 2 ml methanol, 2 ml water Sample application: Suck 1 l water slowly through the column and dry with vacuum Elution: 2 x 2 ml methanol/acetone 1:1, then evaporate at 20 C.
Legend: Substance Recovery rate % Fluometuron 93 Fenuron 83 Monuron 91 Isoproturon 91 Chlortoluron 95 Linuron 106 Diuron 99 Metoxuron 86 Metobromu
Cat. #
730753 730754 730756 730753 G 730755 G Easy Easy Easy Easy - Glass Easy - Glass
Product
60mg 200mg 500mg 60mg 200mg
Weight
3ml 3ml 6ml 3ml 6ml
Size
30 30 30 30 30
Qty/pkg
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CHROMABOND HR-Xpert:
These innovative SPE phases offer:
State-of-the-art spherical polymer allows broad spectrum of applications with special suitability for enrichment of pharmaceuticals from biological matrices Optimized pore structure and high specific phases allows for high load ability, and excellent elution properties, low solvent consumption and rapid analyses High-Purity adsorbent material allows for the highest reproducibility with extremely low blind values, with reliable analyses at ultra trace levels
The innovative concept of five polymer-based RP- and mixed-mode ion exchange phases for SPE:
CHROMABONDHR-X - hydrophobic PS/DVB copolymer CHROMABONDHR-XC - strong mixed-mode cation exchanger CHROMABONDHR-XA - strong mixed-mode anion exchanger CHROMABONDHR-XCW - weak mixed-mode anion cation exchanger CHROMABONDHR-XAW - weak mixed-mode anion exchanger
CHROMABOND HR-Xpert concept neutral, acidic, and basic analytes
strong bases pKa > 10 CHROMABOND HR-XCW
acids pKa 2 - 8 CHROMABOND HR-XA
neutral compounds CHROMABOND HR-X
strong acids pKa > 1 CHROMABOND HR-XAW
bases pKa 2 -10 CHROMABOND HR-XC
Method A
1. Sample pretreatment 2. Column conditioning 3. Sample application 4. Washing 1: (basic) aqueous 5. Washing 2 / Elution 1*: organic 6. Elution 2: organic acidic
Method B
1. Sample pretreatment 2. Column conditioning 3. Sample application 4. Washing 1: aqueous 5. Elution: organic
Method C
1. Sample pretreatment 2. Column conditioning 3. Sample application 4. Washing 1: (acidic) aqueous 5. Washing 2 / Elution 1*: organic 6. Elution 2: organic base
Strong Bases
Acidic Compounds
Neutral Compounds
Strong Acids
Basic Compounds
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HR-X
Properties:
Hydrophobic polystyrene-divinylbenzene copolymer pH stability 1 14 High-purity material with highest reproducibility and lowest blank values due to a novel manufacturing process Spherical particles 85 m; pore size 55 60 Very high surface 1000 m2/g Capacity 390 mg/g (caffeine in water) Excellent recovery rates especially for the enrichment of pharmaceuticals
Pharmaceuticals / active ingredients from tablets, creams and water / waste water Drugs and pharmaceuticals from urine, blood, serum and plasma Trace analysis of pesticides, herbicides, phenols, PAHs and PCBs from
Cat. #
730931 730936 730936 G 730938 732130 HR-X HR-X HR-X - Glass HR-X HR-X - Large Volume
Product
200mg 60mg 60mg 200mg 30mg
Weight
Size
3ml 3ml 3ml 6ml 15ml
Qty/pkg
30 30 30 30 30
HR-XA
Properties:
Base material polystyrene-divinylbenzene copolymer Strong basic quaternary ammonium anion exchanger Exchange capacity 0.25 meq/g, pKa ~ 18 pH stability 1 14 High purity material with highest reproducibility and lowest blank values due to an optimised production process Spherical particles size 85 m; pore size 55 65 Very large specific surface 850 m2/g; pore volume 1.4 cm3/g RP capacity 350 mg/g (caffeine in water) Outstanding recovery rates especially for the enrichment of acidic analytes
Acidic active ingredients from heavily matrix-contaminated samples like e. g. urine, plasma, seru Phenolic acids Acidic herbicides Weak/medium-strength Acids with pKa 2 8
Cat. #
730968 730950 730727 730958 730954 731768 731769 HR-XA HR-XA HR-XA HR-XA HR-XA HR-XA HR-XA
Product
30mg 60mg 100mg 150mg 500mg 155mg 240mg
Weight
Size
1ml 3ml 1ml 6ml 3ml
Qty/pkg
30 30 30 30 30 50 50
HR-XC
Properties:
Base material polystyrene-divinylbenzene copolymer Strong acidic benzenesulphonic acid cation exchanger exchange capacity 1.0 meq/g, pKa < 1 pH stability 1 14 High purity material, highest reproducibility and lowest blank values due to an optimized production process Spherical particles size 85 m; pore size 65 75 Very large specific surface 800 m2/g; pore volume 1.4 cm3/g RP capacity 300 mg/g (caffeine in water) Outstanding recovery rates especially for the enrichment of basic analytes.
Basic active ingredients from heavily matrix-contaminated samples, e.g. urine, plasma, serum Fungicides from food, melamine from milk basic analytes, e. g. amines Bases with pKa 2 10
38
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HR-XC
Cat. #
730969 730956 730049 730957 730953 731755 731756 HR-XC HR-XC HR-XC HR-XC HR-XC HR-XC HR-XC
Product
30mg 60mg 100mg 150mg 500mg 155mg 240mg
Weight
Size
1ml 3ml 1ml 6ml 3ml
Qty/pkg
30 30 30 30 30 50 50
HR-XCW
Properties:
Base material spherical PS/DVB copolymer Weak acidic carboxylic acid cation exchanger Exchange capacity >0.7 meq/g, pKa ~ 5 pH stability 1 14 High purity material, highest reproducibility and lowest blank values due to an optimised production process Spherical particles size 85 m; pore size 50 60 Very large specific surface 850 m2/g; pore volume 1.2 1.4 cm3/g RP capacity 350 mg/g (caffeine in water) Outstanding recovery rates especially for enrichment of strongly basic analytes
Basic compounds like Quaternary amines Active ingredients from Heavily matrix-contaminated, samples e.g. urine, plasma, serum Strong bases with pKa > 10
Cat. #
730731 730735 730733 730737 730741 731774 731775 HR-XCW HR-XCW HR-XCW HR-XCW HR-XCW HR-XCW HR-XCW
Product
30mg 60mg 100mg 150mg 500mg 155mg 240mg
Weight
Size
1ml 3ml 1ml 6ml 3ml
Qty/pkg
30 30 30 30 30 50 50
HR-XAW
Properties:
Base material spherical PS/DVB copolymer Weak basic secondary and tertiary ammonium anion exchanger, exchange capacity >0.5 meq/g, pKa ~ 6 pH stability 1 14 High purity material with highest reproducibility and Lowest blank values due to an optimised production process Spherical particles size 85 m; pore size 55 65 Very large specific surface 850 m2/g; pore volume 1.2 1.4 cm3/g RP capacity 350 mg/g (caffeine in water) Outstanding recovery rates especially for enrichment of acidic analytes
Weak basic secondary and tertiary ammonium anion Exchanger, exchange capacity >0.5 meq/g, pKa ~ 6 Base material spherical PS/DVB copolymer pH stability 1 14 High purity material with highest reproducibility and Lowest blank values due to an optimised production process Spherical particles size 85 m; pore size 55 65 Very large specific surface 850 m2/g; pore volume 1.2 1.4 cm3/g RP capacity 350 mg/g (caffeine in water) Outstanding recovery rates especially for enrichment of acidic analytes
Cat. #
730728 730747 730729 730749 730744 731771 731772 HR-XAW HR-XAW HR-XAW HR-XAW HR-XAW HR-XAW HR-XAW
Product
30mg 60mg 100mg 150mg 500mg 155mg 240mg
Weight
Size
1ml 3ml 1ml 6ml 3ml
Qty/pkg
30 30 30 30 30 50 50
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39
SPE Cartridges - Jordi

Jordi brings you the highest quality 100% divinylbenzene (DVB) cartridges available. These resins are designed with a 10m average particle size compared to the typical 40m of leading brands. This smaller particle size provides extremely high retention efficiency and higher capacity. Jordi cartridges offer a significantly larger useful surface area while maintaining excellent flow characteristics. Jordi offers the broadest range of polymer-based surface chemistries on the market, addressing aqueous, organic and ion exchange sample preparation. Jordi resins are made from 100% divinylbenzene, making them more retentive than standard PS-DVB packings. This makes Jordi Hydroclean SPE one of the most retentive phases available, providing increased analyte recoveries. Jordi Quality Assurance All Jordi SPE products are manufactured with the highest quality DVB and checked by stringent standards. Quality control for all of Jordis materials include: Particle Size - Electrozone sensing analysis of particle size distribution Particle Shape - Light microscopy analysis of particle shape and size Surface area and Porosity - Nitrogen adsorption Chromatographic performance - Column chromatography Purity - Mass spectroscopy (MS)
Advantages of Polymeric SPE

Outstanding recovery due to the highly retentive nature of polymeric phases Absence of leachables from the polymeric media since there is no bonded phase Lack of residual silanol groups Incredible ruggedness of polymeric supports allows wide pH and solvent compatibility Improved batch to batch reproducibility Higher capacity for improved detection limits and reduced resin volumes
Hydroclean RP
Localized hydrophobic/hydrophilic balance for optimal analyte/resin contact and retention Patent pending polyamide stationary phase allows for NP and RP separations in 100% water or 100% organic solvent Exhibits improved wettability even after drying
Hydroclean SCX
Sulfonated stationary phase allows for cation exchange separations 100% DVB supports promote increased durability, excellent flow properties and stability throughout the entire pH scale SCX exhibits excellent wettability even after drying due to hydrophilic/hydrophobic balance
Hydroclean SAX
Quaternary amine phase allows for anion exchange separations Features 100% DVB supports, for enhanced retention characteristics SAX exhibits excellent wettability even after drying due to hydrophilic/hydrophobic balance
Hydroclean RP DVB
100% Divinylbenzene phase Features enhanced retention over styrene/DVB phases DVB the phase of choice for highest retention
40
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Melamine with Milk
Plasticizers and UV Screens
Detection Method Packing: Jordi Gel DVB 500 Column: 4.6mm x 150mm Cat. #: 16502 Mobile Phase: 10/20/30/40 MeOH/CAN/THF/H2O Flow Rate: 1 ml/min Detection: UV238nm Analyte: Melamine Conc.: 1 mg/ml Recovery: 93.1 %
Procedure
Product: Cat. #: Cartridge Prep: Sample Loading: Wash: Elution: Matrix: HRP-SCX 200mg 6ml 500SPE-006-00200 Solvent 1 Methanol (3ml) Solvent 3 Hydrochloric Acid (3ml, 0.1 M) 4ml of total solution Wash 1 Hydrochloric Acid (3ml, 0.1M) Wash 2 (75/25) Methanol / Hydrochloric Acid (0.1M) (3ml) Dry Step 10 mm Mercury (~ 5 min.) (95:5) Methanol / NH4OH (3ml) Milk diluted 3:1 with 0.1M HCl Melamine at 0.25 mg/ml
Procedure
Product: Sample Loading: Wash: Elution: HRP 200mg 6ml Cat. #: 530SPE-006-00200 The solution was completely loaded and was allowed to pull to dryness Wash Solvent 1 Methanol (2ml) Wash Solvent 2 (75/25) Water Methanol (3ml) 2ml Methanol
Analytes
Triethylcitrate Diethylphthalate Dioctylphthalate 6-Undecanone Hexadecanol
ppm(1/25 dilution)
35 30 26 29 34 33 98 106 100 102 109 79
% Recovery
Jordi SPE Cartridges

Cat. #
500SPE-001-00030 500SPE-003-00060 500SPE-006-00200 500SPE-961-00030 510SPE-001-00030 510SPE-003-00060 510SPE-006-00200 510SPE-961-00030 530SPE-001-00030 530SPE-003-00060 530SPE-006-00200 530SPE-961-00030 580SPE-001-00030 580SPE-003-00060 580SPE-006-00200 580SPE-961-00030
Padimate O
Description
Hydroclean SCX Hydroclean SCX Hydroclean SCX Hydroclean SCX - 96 Well Hydroclean RP DVB Hydroclean RP DVB Hydroclean RP DVB Hydroclean RP DVB - 96 Well Hydroclean RP Hydroclean RP Hydroclean RP Hydroclean RP - 96 Well Hydroclean SAX Hydroclean SAX Hydroclean SAX Hydroclean SAX - 96 Well
Pore Size
80 80 80 80 80 80 80 80 80 80 80 80 80 80 80 80
Part. Size
25-35m 25-35m 25-35m 25-35m 25-35m 25-35m 25-35m 25-35m 25-35m 25-35m 25-35m 25-35m 25-35m 25-35m 25-35m 25-35m
Size
1ml 3ml 6ml 1ml 1ml 3ml 6ml 1ml 1ml 3ml 6ml 1ml 1ml 3ml 6ml 1ml
Bed Weight
30mg 60mg 200mg 30mg 30mg 60mg 200mg 30mg 30mg 60mg 200mg 30mg 30mg 60mg 200mg 30mg
Qty/pkg
100 100 30 1 100 100 30 1 100 100 30 1 100 100 30 1
TLC Plates
Thin Layer Chromatography
Sorbent Technologies offers the highest performance and widest selection of TLC plates on glass, plastic, and aluminum supports. Analytical and preparative layers with different sorbent coatings: silica gel, alumina, cellulose, bonded phases, polyamide, chiral, and more are available. TLC plates are available in a wide array of sizes from 20 x 20cm to 2.5 x 7.5cm with or without UV254 indicator. HPTLC plates provide higher performance and better resolution. Inquire about custom plates. We also carry TLC accessories.
Quality
Our TLC plates are guaranteed by stringent production control including standardized lot tests, surface checks for roughness or cracks as well as hardness and adherence checks. Plates and sheets are immediately ready for chromatographic separation and require no coatings or impregnations. TLC layers are smooth, homogenous and well-adhering to produce reproducible quantitative evaluations.
TLC/HPTLC Advantages:
High sample throughput in a short time Suitable for screening tests Pilot Procedure for HPLC and flash chromatography After separation the analytical information can be stored for a longer period of time (the TLC plate acts as a storage medium for data) Separated substances can be subjected to subsequent analytical procedures (e.g. IR, MS) at a later date. Rapid and cost effective optimization of the separation due to easy change of mobile and stationary phase.
TLC Plate and Sheet Characteristics:
Classical Adsorbents - for ~80% of all TLC separations silica 60 (mean pore diameter of 60 = 6nm) is used. Other classical adsorbents are aluminium oxide, cellulose, diatomaceous earth, ion exchangers and polyamide. Special Phases - reversed phases, mainly C18 (octadecyl) modified silica, but also cyano-, amino-, diol and C2 modified silica are available. Special layers for specific separations, like our Chiral TLC plate for enantiometer separation complete the versatile range of TLC plates. Particle Size Distribution and Thickness of Layer Particle size distribution and thickness of layer are chosen to fit the given type of application (e.g. HPTLC, standard or preventative separations). Most ready-to-use layers are available with or without fluorescent indicator.
TLC Plates Conditioning and Cutting

Standardization
The following conditioning procedure allows proper standardization of TLC plate performance for reproducibility from chemist to chemist and plate to plate. 1. Pre-wash your plate to remove impurities: this will result in a more reproducible separation and purer extracted components. a. General pre-washing: use methanol or methanol/chloroform (1:1). Develop overnight to top of plate. Let air dry in a ventilation hood. b. Extraction pre-washing: use the solvent system that you are going to use for the extraction of the fractions after separation. Develop overnight to top of plate. Let air dry in a ventilation hood. 2. Activation strengthens the layer and increases reproducibility. a. Dry in a vacuum oven at 110C for an hour b. Let the plates cool under vacuum until room temperature c. Store in a desiccator until use
Cutting Tips
Flexible Plastic and Aluminum Backed Plates:

1. Keep your plates as dry as possible, storing in a desiccator if possible (if not in a desiccator, keep the box closed at all times). 2. Using very sharp scissors, cut at a 45 angle leaning to the outside (therefore, if you are right handed, the cut should lean to the right)
Glass Backed Plates:

1. Buy the SorbTech TLC Plate Glass Cutter (Cat.# 7535) to enable you to optimize plate cutting for analytical and prep TLC plates. This will save both time and money from material waste and labor.
42
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Extra Hard Layers - Silica XHL TLC

Silica Gel Extra Hard Layer (XHL) plates feature a newly developed binder system, resulting in an outstanding hardness of the silica layer. Due to its excellent abrasion resistance, the formation of dust on the surface of XHL Silica is minimized. Several advantages of the Silica XHL TLC: The plate can be easily labeled with a lead pencil for clear identification. The high abrasion resistance prevents undesired changes or damage to the layer (thickness) during transportation or handling. A dust-free plate allows convenient handling and avoids cross-contamination of the TLC chamber. The UV indicator of the XHL Silica features an increased detection sensitivity. The increased sensitivity allows for successful applications in trace analysis. Compared to Hard Layer (HL) and Soft Layer (SL) Silica plates, Extra Hard Layer (XHL) Silica demonstrates improved separation efficiency, since the particle size distribution of the silica was optimized. The selectivity of XHL Silica is different compared to HL and SL Silica. Thus the chromatographer now can choose from 3 different types of Silica plates (XHL, HL, and SL) in order to optimize a given chromatographic experiment.
Cat. #
4115026 4115056 4115067 4115126 4115137 4115148 4115156 4115167 4115198 4214137 4214156 4214178
Layer
Silica XHL Silica XHL Silica XHL Silica XHL Silica XHL Silica XHL Silica XHL Silica XHL Silica XHL Nano-Silica XHL Nano-Silica XHL Nano-Silica XHL
Backing
glass glass glass glass glass glass glass glass glass glass glass glass
Thickness
250m 250m 250m 250m 250m 250m 250m 250m 250m 200m 200m 200m -
Indicator
Size (cm)
5x10 10x10 20x20 20 x 20 10 x 20 5 x 20 10 x 10 5 x 10 2.5 x 7.5 10 x 20 10 x 10 5x5 50 25 25 25 50 100 25 50 100 50 25 100
Qty/pkg
UV254 UV254 UV254 UV254 UV254 UV254 UV254 UV254 UV254
For more selections and sizes, visit our website or contact your personal Product Specialist 43 Toll Free: 866.SORBTEC Fax: 770.936.0326 www.sorbtech.com
Extra Hard Layers - Silica HL TLC

Silica Hard Layer (HL) is a family of pre-coated glass plates with unmodified silica. It is available in two types as Silica Hard Layer for TLC and Silica Hard Layer for HPTLC. Silica Hard Layer plates differ from the well-known Silica Soft Layer TLC and HPTLC by a special binder system, resulting in the formation of hard, water-resistant and wettable layers with outstanding separation properties and consistent reproducibility from lot to lot. Like our proven Silica Soft Layer TLC plates, HL TLC Plates are coated with unmodified silica 60 . They are available with or without fluorescent indicator (green fluorescence, 254 nm). If one compares the performance of the HL TLC plates with the conventional Silica Soft Layer TLC plates for the separation of anthraquinone dyes (see pg 45), one observes a similar selectivity, but with different Rf values. A closer observation reveals the slight differences in selectivity for the different layers. For a normal phase separation of anthraquinone dyes, HL TLC and Silica Soft Layer TLC plates behave quite similar (see fig. A). Under reversed phase conditions (fig. B), the different characteristics of the two plates become obvious. While the Silica Soft Layer TLC Plate shows some reversed phase properties due to the still lower polarity of the binder system, HL TLC is a true normal phase TLC plate.
Cat. #
2115126 2115137 2115148 2115169 2115167 2115026 2115048 2115037 2115067
Layer
Silica HL Silica HL Silica HL Silica HL Silica HL Silica HL Silica HL Silica HL Silica HL
Backing
glass glass glass glass glass glass glass glass glass
Thickness
250m 250m 250m 250m 250m 250m 250m 250m 250m
Indicator
UV254 UV254 UV254 UV254 UV254 -
Size (cm)
20 x 20 10 x 20 5 x 20 5 x 10 5 x 10 20 x 20 5 x 20 10 x 20 5 x 10 25 50 100 200 50 25 100 50 50
Qty/pkg
Soft Layers - Silica XG TLC

Silica XG layers have a newly developed binding agent that makes it adhere to the aluminum substrate more strongly than the Silica G layers. This equates to less flaking of the coating. For these TLC plates a silica gel with a 60 mean pore diameter, a 500m2/g specific surface area, a 0.75 ml/g specific pore volume, and a particle size of 5-17um is used. Fluorescent indicator (UV254) and pre-cut sizes are available. Silica XG: This is a soft layer plate that contains Gypsum.
Cat. #
4434126 4434135 4434147 4434167 44341C5 4434187 4434026 4434047 4434067 44340C5
Layer
Silica XG Silica XG Silica XG Silica XG Silica XG Silica XG Silica XG Silica XG Silica XG Silica XG
Backing
aluminum aluminum aluminum aluminum aluminum aluminum aluminum aluminum aluminum aluminum
Thickness
200m 200m 200m 200m 200m 200m 200m 200m 200m 200m
Indicator
UV254 UV254 UV 254 UV254 UV254 UV254 -
Size (cm)
20 x 20 10 x 20 5 x 20 5 x 10 5 x 7.5 4x8 20 x 20 5 x 20 5 x 10 5 x 7.5 25 20 50 50 20 50 25 50 50 20
Qty/pkg
44
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Soft Layers - Silica G TLC

Silica Gel, 60, 5 - 17m Analytical and Preparative Layers (slightly larger particles are used) Indicator: UV254 - manganese activated zinc silicate with green fluorescence Polymeric binder which is stable in almost all organic solvents and resistant towards aggressive visualization reagents Binder for polyester backed plates is completely stable in purely aqueous eluents.
Analysis of edible fats

Plate: Silica G, w/UV254, polyester backed, 200um, 20x20cm, 25/pk Phase: Silica G Catalog #: 1624126 Substances: cholesterol triglycerides cholesterol ester Information: be used without further treatment. Conditions: Eluents: Dichloromethane-toluene 5:2 (v/v) Sample volume: 0.5 l Migration distance: 6 cm Detection: Spray with a ethanolic solution of molybdatophosphoric acid, then dry with hot air. Heat the sheet with a hot air gun for 3-4 min.
Separation of Anthraquinone Dyes Layers: a) SIL G-25 UV254 Eluent: a) normal phase TLC: toluene/cylcohexane (2:1. v/v) b) reversed phase TLC: acetone/water (1:1. v/v) Migration distance: a) 4.8 cm in 8 min b) 3.8 cm in 10 min Detection: TLC scanner, UV 254 nm Peaks: 1.blue 2. Violet 2 3. Blue 1 4. secondary spot of blue
Legend: Compounds Rf Cholesterol 0.16 Triglycerides 0.54-0.66 Cholesterol esters 0.95
Cat. #
1624126 1624147 1624187 1624199 1634126 1634147 1634187 1634199 1615026 1615126 1615148 1615167 1615190 1616125 1617124 1618123
Layer
Silica G Silica G Silica G Silica G Silica G Silica G Silica G Silica G Silica G Silica G Silica G Silica G Silica G Silica G-Prep Silica G-Prep Silica G-Prep
Backing
polyester polyester polyester polyester aluminum aluminum aluminum aluminum glass glass glass glass glass glass glass glass
Thickness
200m 200m 200m 200m 200m 200m 200m 200m 250m 250m 250m 250m 250m 500m 1000m 2000m
Indicator
UV254 UV254 UV254 UV254 UV254 UV254 UV254 UV254 UV254 UV254 UV254 UV254 UV254 UV254 UV254
Size (cm)
20 x 20 5 x 20 4x8 2.5 x 7.5 20 x 20 5 x 20 4x8 2.5 x 7.5 20 x 20 20 x 20 5 x 20 5 x 10 2.5 x 7.5 20 x 20 20 x 20 20 x 20 25 50 50 200 25 50 50 200 25 25 100 50 500 20 15 12
Qty/pkg
Soft Layers - Silica Concentrating Zone TLC Plates

The concentrating zone serves as a quick application zone, which allows quantitative evaluation of chromatograms, even if samples have been applied with an unsteady hand. Since careful sample application is time-consuming or requires special equipment, silica plates with concentrating zone are real time-savers. This includes economy in preparation time because of a reduction in evaporation time: larger volumes of dilute solutions can be applied instead of smaller quantities of concentrated solutions. This is often of advantage especially in biochemical investigations.
Cat. #
2315126 2315137 2315026 2315037
Layer
Silgur (Silica 60 w/ Diatomaceous earth zone) Silgur (Silica 60 w/ Diatomaceous earth zone) Silgur (Silica 60 w/ Diatomaceous earth zone) Silgur (Silica 60 w/ Diatomaceous earth zone)
Backing
glass glass glass glass
Thickness
250m 250m 250m 250m
Indicator
UV254 UV254 -
Size (cm)
20 x 20 10 x 20 20 x 20 10 x 20 25 50 25 50
Qty/pkg
Cellulose - Cellulose Layers for TLC Plates

Cellulose 300
Properties: Fibre length (95 %) 2 20 m, average degree of polymerisation 400 500, specific surface acc. to Blaine 15000 cm2/g 20 ppm Fe, 6 ppm Cu, 7 ppm P; CH2Cl2 extract 0.25 %; residue on ignition at 850 C 1500 ppm Application: Partition chromatography of polar substances such as amino acids, carboxylic acids or carbohydrates
Cat. #
0314026 0314126 0315026 0315126 0322126 0332126
Layer
Cellulose 300 Cellulose 300 Cellulose 300 Cellulose 300 Cellulose 300 Cellulose 300
Backing
glass glass glass glass polyester aluminum
Thickness
0.10mm 0.10mm 0.25mm 0.25mm 0.25mm 0.25mm -
Indicator
UV254 UV254 UV254 UV254
Size (cm)
20 x 20 20 x 20 20 x 20 20 x 20 20 x 20 20 x 20 25 25 25 25 25 25
Qty/pkg
Cellulose 300 PEI
Properties: Fibrous cellulose impregnated with polyethyleneimine Application: analysis of nucleic acids, and of mutagenic substances with the 32P postlabelling procedure (see application 402260 at www.mn-net.com)
Cat. #
1122026 1122126
Layer
Cellulose 300 PEI Cellulose 300 PEI
Backing
polyester polyester
Thickness
0.10mm 0.10mm -
Indicator
UV254
Size (cm)
20 x 20 20 x 20 25 25
Qty/pkg
Cellulose 400
Properties: Prepared: by hydrolysis of high purity cellulose with HCL; mean degree of polymerisation 40-200 Application: Carboxylic acids, lower acids, lower alcohols, urea and purine derivatives
Cat. #
0412126 0422126
Layer
Cellulose 300 PEI Cellulose 300 PEI
Backing
glass polyester
Thickness
0.10mm 0.10mm
Indicator
UV254 UV254
Size (cm)
20 x 20 20 x 20 25 25
Qty/pkg
46
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C18 Silica TLC

Base material: Silica Gel, 60, mean particle size 9m, pH stability 2 - 10 Indicator: UV254 - Acid-resistant product with a pale blue fluorescence; UV-absorbing substances appear as dark-blue to black spots on a light-blue background Partial octadecyl (C18) modification, wettable with water, carbon content 14% Normal phase or reversed phase separation modes with eluents from anhydrous solvents to mixtures with high concentrations of water Recommended applications: aminophenols, barbiturates, preservatives, nucleobases, polycyclic aromatic hydrocarbons, steroids, tetracyclines, plasticizers (phthalates)
Cat. #
2715126 2715137 2715147 2715156 2717124 2733126 2733147 2733156 2733167 2733187
Layer
C18 Silica C18 Silica C18 Silica C18 Silica C18 Silica C18 Silica C18 Silica C18 Silica C18 Silica C18 Silica
Backing
glass glass glass glass glass aluminum aluminum aluminum aluminum aluminum
Thickness
250m 250m 250m 250m 1000m 150m 150m 150m 150m 150m
Indicator
UV254 UV254 UV254 UV254 UV254 UV254 UV254 UV254 UV254 UV254
Size (cm)
20 x 20 10 x 20 5 x 20 10 x 10 20 x 20 20 x 20 5 x 20 10 x 10 5 x 10 4x8 25 50 50 25 15 25 50 25 50 50
Qty/pkg
C2 Silica TLC
Base material: Silica Gel, 60, particle size 5 - 17m, pH stability 2 - 10 Indicator: UV254 - Acid-resistant product with a pale blue fluorescence; UV-absorbing substances appear as dark-blue to black spots on a light-blue background Silanized silica with dimethyl modification, carbon content 4% Recommended applications: active plant constituents, steroids
Cat. #
2815126 0314126 0315026 0315126 C2 Silica C2 Silica C2 Silica C2 Silica
Layer
Backing
glass glass aluminum aluminum
Thickness
250m 250m 150m 150m
Indicator
UV254 UV254 UV254 UV254
Size (cm)
20 x 20 10 x 10 20 x 20 4x8 25 25 25 50
Qty/pkg
TLC Plates - Thin Layer Chromatography

Cyano Nano- Silica HPTLC
Base material: Silica Gel, 60, particle size 2 - 10m, pH stability 2 - 8 Indicator: UV254 - Acid-resistant product with a pale blue fluorescence; UV-absorbing substances appear as dark-blue to black spots on a light-blue background Cyanopropyl modification, carbon content 5.5% Normal phase or reversed phase separation modes depending on the polarity of the developing solvent Recommended applications: steroid hormones, phenols, preservatives
Cat. #
3114126 3114136 3114156 3133126 3133187
Layer
Cyano Nano-Silica Cyano Nano-Silica Cyano Nano-Silica Cyano Nano-Silica Cyano Nano-Silica
Backing
glass glass glass aluminum aluminum
Thickness
200m 200m 200m 150m 150m
Indicator
UV254 UV254 UV254 UV254 UV254
Size (cm)
20 x 20 10 x 20 10 x 10 20 x 20 4x8 25 25 25 25 50
Qty/pkg
Amino Nano- Silica HPTLC

Base material: Silica Gel, 60, particle size 2 - 10m, pH stability 2 - 8 Indicator: UV254 - Acid-resistant product with a pale blue fluorescence; UV-absorbing substances appear as dark-blue to black spots on a light-blue background Aminopropyl modification, carbon content 3.5% Layer can be wetted equally well by pure water as by organic solvents Recommended applications: vitamins, sugars, steroids, purine derivatives, xanthines, phenols, nucleotides and pesticides
Cat. #
3014136 3014156 3033187
Layer
Amino Nano-Silica Amino Nano-Silica Amino Nano-Silica
Backing
glass glass aluminum
Thickness
200m 200m 200m
Indicator
UV254 UV254 UV254
Size (cm)
10 x 20 10 x 10 4x8 25 25 50
Qty/pkg
48
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Diol Nano- Silica HPTLC

Base material: Silica Gel, 60, particle size 2 - 10m, pH stability 2 - 8 Indicator: UV254 - Acid-resistant product with a pale blue fluorescence; UV-absorbing substances appear as dark-blue to black spots on a light-blue background Diol modification, carbon content 5.5% Layer can be wetted equally well by pure water as by organic solvents Recommended applications: steroids, pesticides and plant constituents; for more critical separations an alternative to silica, since it is less sensitive to water content in the environment; leads to more reproducible results compared to silica
Cat. #
2914156 2933187
Layer
Diol Nano-Silica Diol Nano-Silica
Backing
glass aluminum
Thickness
200m 150m
Indicator
UV254 UV254
Size (cm)
10 x 10 4x8 25 50
Qty/pkg
Alumina TLC
Aluminum oxide Indicator: UV254 - manganese activated zinc silicate with green fluorescence Recommended applications: terpenes, alkaloids, steroids, aliphatic and aromatic compounds We recommend heating the plates for 10 min. at 120C before usage to activate the alumina layer.
Cat. #
0115126 0115148 0117134 0224126 0224147 0224187 0224199 0234126 0234147 Alumina Alumina Alumina Alumina Alumina Alumina Alumina Alumina Alumina
Layer
Backing
glass glass glass polyester polyester polyester polyester aluminum aluminum
Thickness
250m 250m 1000m 200m 200m 200m 200m 200m 200m
Indicator
UV254 UV254 UV254 UV254 UV254 UV254 UV254 UV254 UV254
Size (cm)
20 x 20 5 x 20 20 x 20 20 x 20 5 x 20 4x8 2.5 x 7.5 20 x 20 5 x 20 25
Qty/pkg
100 15 25 50 50 200 25 50
TLC Plates - Thin Layer Chromatography

Chiral TLC
Sorbtech offers TLC Chiral plates: reverse phase silica gel impregnated with a chrial selector and copper ions. This allows for multiple simultaneous separations an ideal plate for routine applications and production control. The separation of optically active isomers is based on ligand exchange. The chrial selector on the plate and the enantiomers to be separated form diastereomeric mixed chelate complexes with the transition metal ion. Complexes for the different antipodes have different stabilities thus achieving chromatographic separation. Quantitative determination (remission location curves) of TLCseparated enantiomers of tert.-leucine: Layer: CHIRAL TLC Eluent: methanol water (10:80, v/v) Detection: dip in 0.3% ninhydrin solution quantification with scanner, 520 nm a) L-tert.-leucine, b) L-tert.-leucine + 0.1% D-tert.-leucine, c) L-tert.-leucine + 1 % D-tert.-leucine, d) external reference sample
Cat. #
3415126 3415136 3415131 3415147 3415156 Chiral Chiral Chiral Chiral Chiral
Layer
Backing
glass glass glass glass glass
Thickness
250m 250m 250m 250m 250m
Indicator
UV254 UV254 UV254 UV254 UV254
Size (cm)
20 x 20 10 x 20 10 x 20 5 x 20 10 x 10 25 25 4 50 25
Qty/pkg
TLC Accessories
Developing Chamber Glass Capillaries TLC Spotting Guide
Every lab needs a TLC Glass Plate Cutter

Save Time
Just score and snap off High quality carbide scriber is mounted into a movable plastic head for optimal, one-finger scoring Virtually maintenance-free, a standard screwdriver disassembles cutter for easy cleaning and care
Cat.# 814019
Cat.# 814022
Cat.# 814023
Save Money
No need to share experiments in the classroom Use only what you need, small runs are now cost effective Benefit from bulk purchases then select your size
Cat.# 7535
50
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Flash Cartridges
Sorbtech High Performance Empty Flash Cartridges
Sorbent Technologies EZ Flash MT Cartridges allow you to easily pack your own flash cartridges with any resin of your choice: silica gel, C18, Amino, Polymeric and more! Solvent resistant polypropylene cartridges are available in 5 sizes: 8, 25, 80, 170, & 230 ml. This is equivalent to 4, 12, 40, 80, & 120 grams of packed flash grade silica gel.
Cartridges are rated at 150 psi and easily connect to any Flash System with luer connect end fittings. Luer End Fittings allow for easy connection to any flash system Top & Bottom Frit Included (replacement frits available) Screw Cap allows for easy assembly Pressure Rated to 150 PSI Easy to Pack
Standard female luer
Standard male luer
Catalog #
FCST-4-20 FCST-12-20 FCST-40-20 FCST-80-20 FCST-120-16
Description
Flash Cartridge, Screw Top Flash Cartridge, Screw Top Flash Cartridge, Screw Top Flash Cartridge, Screw Top Flash Cartridge, Screw Top
Dimensions (L x I.D.)
77 x 13mm 97 x 20.25mm 150 x 24.56mm 220 x 33.15mm 246 x 33.55mm 4g 12g 40g 80g
Weight*
Vol.
8ml 25ml 80ml 170ml 230ml
Qty/pkg
20/pk 20/pk 20/pk 20/pk 16/pk
120g
*Sorbent weight is based on Silica as a reference Bulk packaging & pricing available. For more selections and sizes, visit our website or contact your personal Product Specialist Sorbent Technologies, Inc. Toll Free: 866.SORBTEC Fax: 770.936.0326 www.sorbtech.com 51
NEW! Introducing Sorbtech Purity Flash Cartridges

Purity Flash Cartridges offer the best speed, resolution, and greater loading capacity. These rugged cartridges have increased durability to withstand higher pressures (up to 200 psi). Packed with Premium RF Silica Gel (available for Flash 40-75m and MPLC 20-45m), Purity Flash Cartridges offer tighter particle distribution and minimal fines, keeping back pressure low. The cartridges are compatible with the Sorbtech EZ Flash System, Teledyne Isco, Biotage, Agilent Technologies, Interchim, and Grace Discovery Sciences systems.
Purity Flash Cartridges (40-75m)

Packed with Premium RF Silica Gel Narrow particle distribution: 40-75m Small pore volume: Higher bulk density. Narrow pore size distribution: Minimum small pores. Purity: 99.95% SiO2 Superior Packing Technique Uniform packed bed reducing channeling Higher resolution and sharper peaks Standard Luer-Lock end fittings Easy connection to commercially available flash systems Inventive cartirdge design. Versatile Leak-Free Spin-welded allowing higher pressure up to 300psi 100% compatible with Biotage, Teledyne Isco, and Agilent Flash Systems
Cat. #
CFC-52500-004-20 CFC-52500-012-18 CFC-52500-025-12 CFC-52500-040-12 CFC-52500-080-10 CFC-52500-120-10 CFC-52500-220-6 CFC-52500-330-5
Model
Flash Cartridges, Silica Gel, Premium Rf, 60, 40-75m Flash Cartridges, Silica Gel, Premium Rf, 60, 40-75m Flash Cartridges, Silica Gel, Premium Rf, 60, 40-75m Flash Cartridges, Silica Gel, Premium Rf, 60, 40-75m Flash Cartridges, Silica Gel, Premium Rf, 60, 40-75m Flash Cartridges, Silica Gel, Premium Rf, 60, 40-75m Flash Cartridges, Silica Gel, Premium Rf, 60, 40-75m Flash Cartridges, Silica Gel, Premium Rf, 60, 40-75m
Column Size
4g 12g 25g 40g 80g 120g 220g 330g
Dimmensions (DxL) (inner D)

17.2 x 113.8 (12.4) 27.8 x 134.8 (21.4) 28.0 x 184.0 (21.4) 34.2 x 184.4 (26.7) 39.0 x 257.4 (31.2) 48.0 x 261.5 (38.6) 71.4 x 223.5 (61.4) 71.6 x 280.2 (61.4)
Sample Load
4mg-0.4g 12mg-1.2g 25mg-2.5g 40mg-4.0g 80mg-8.0g 120mg-12g 220mg-22g 330mg-33g
Qty/ pkg
20 18 12 12 10 10 6 5
Cat. #
Model
Flow Rate
15-40ml/min 30-60ml/min 30-60ml/min 40-70ml/min 50-100ml/min 60-150ml/min 80-220ml/min 80-220ml/min
Max Press.
300 psi (21 Bar) 300 psi (21 Bar) 300 psi (21 Bar) 300 psi (21 Bar) 300 psi (21 Bar) 300 psi (21 Bar) 250 psi (17 Bar) 250 psi (17 Bar)
Column Vol.
5ml 19ml 32ml 48ml 108ml 160ml 303ml 420ml
Cartridge Material
PE, USP Class VI PE, USP Class VI PE, USP Class VI PE, USP Class VI PE, USP Class VI PE, USP Class VI PE, USP Class VI PE, USP Class VI
52
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Flash Cartridges - Sorbtech

Purity Flash Cartridges (20-45m)
Packed with Premium RF Silica Gel Narrow particle distribution: 20-45m Small pore volume: Higher bulk density. Narrow pore size distribution: Minimum small pores. Purity: 99.95% SiO2 Superior Packing Technique Uniform packed bed reducing channeling Higher resolution and sharper peaks Standard Luer-Lock end fittings Easy connection to commercially available flash systems Inventive cartirdge design Versatile Leak-Free Spin-welded allowing higher pressure up to 300psi 100% compatible with Biotage, Teledyne Isco, and Agilent Flash Systems
Cat. #
Model
Column Size
4g 12g 25g 40g 80g 120g 220g 330g
Dimmensions (DxL) (inner D)

17.2 x 113.8 (12.4) 27.8 x 134.8 (21.4) 28.0 x 184.0 (21.4) 34.2 x 184.4 (26.7) 39.0 x 257.4 (31.2) 48.0 x 261.5 (38.6) 71.4 x 223.5 (61.4) 71.6 x 280.2 (61.4)
Sample Load
4mg-0.6g 12mg-1.8g 25mg-3.8g 40mg-6.0g 80mg-12g 120mg-18g 220mg-33g 330mg-50g
Qty/ pkg
20 18 12 12 10 10 6 5
Cat. #
Model
Flow Rate
15-40ml/min 30-60ml/min 30-60ml/min 40-70ml/min 50-100ml/min 60-150ml/min 80-220ml/min 80-220ml/min
Max Press.
300 psi (21 Bar) 300 psi (21 Bar) 300 psi (21 Bar) 300 psi (21 Bar) 300 psi (21 Bar) 300 psi (21 Bar) 250 psi (17 Bar) 250 psi (17 Bar)
Column Vol.
5ml 19ml 32ml 48ml 108ml 160ml 303ml 420ml
Cartridge Material
PE, USP Class VI PE, USP Class VI PE, USP Class VI PE, USP Class VI PE, USP Class VI PE, USP Class VI PE, USP Class VI PE, USP Class VI
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53
Flash Cartridges - Agilent Technologies

F75 & F150 Cartridges
Specially designed slotted dispersion discs improve flow properties High quality, precise tolerance machining Superior packing through our exclusive Gradient Free Compression technology - you can actually hear the difference! Certified consistency to meet your GMP needs Improved seal provides less leaking and opportunity for cartridge sealing & reuse (when used with Analogix compression modules) Top performance at a competitive price 100% compatible with Biotage Flash Systems
The F75TM Cartridges come in three standard sizes.
Advantages of Flash Cartridges vs. Glass Columns

Faster Run Time: 2 - 10 times faster Ready for GMP Certification and Compliance Increase Sample Loading: 2 - 10 xs greater Decrease Labor Cost & Saves Time associated with packing, cleaning, and running of column Increase Sample Yield & Productivity Better Separation & Resolution Safer/Eliminates Hazards due to handling and disposing of sorbents, packing, cleaning, and breaking of glass columns Ensures Reproducibility from Run to Run Utilize Less Solvent
The F150TM Cartridges come in a standard package size of 2 cartridges.
Cat. #
AX0346-2 AX0347-2 AX0348-2 AX0355-2 AX0356-2
Model
F75STM F75MTM F75L
TM
Bed Mass
200g 400g 800g 2.5kg 5.0kg
Dim. (DxL)
7.5 x 9cm 7.5 x 18cm 7.5 x 36cm 15 x 30cm 15 x 60cm
Flow Rate
250ml/min 250ml/min 250ml/min 0.5-1.0 L/min 0.5-1.0 L/min
Max Press.
100 psi 100 psi 100 psi 100 psi 100 psi
Sample Load
1.5-10g 2.5-20g 5-40g 18-125g 38-320g
Column Volume
320ml 535ml 1.07L 30L 60L
Cartridge Material
PE, USP Class VI PE, USP Class VI PE, USP Class VI PE, USP Class VI PE, USP Class VI
Qty/pkg
2 2 2 2 2
F150MTM F150L
TM
Note: Cartridges are available in bulk packs of 10. Cartridges are available with all types of phases, (e.g. Silica, C18, Alumina, etc.) Inquire about custom packings. 54 Sorbent Technologies, Inc. Toll Free: 866.SORBTEC Fax: 770.936.0326 www.sorbtech.com
Flash Cartridges - Agilent Technologies

Dissolve Absorb Sample Injection (DASi)
Available in three adjustable sizes, the DASi is a flexible, easy-to-use device for loading low-solubility and high-viscosity compounds on a purification column. Standard luer fittings enable connection to any compound purification system either directly or with the addition of simple conversion kits.
Cat. #
AX1236-1 AX1246-20 AX1246-100 AX1253-20 AX1253-100
Description
DASi 65 Sample Loading Module Kit (For loading insoluble samples) DASi 65g Empty Sample Load Cartridges w/Frits, Normal Phase, 20/pk DASi 65g Empty Sample Load Cartridges w/Frits, Normal Phase, 100/pk DASi 65g Empty Sample Load Cartridges w/Frits, Reverse Phase, 20/pk DASi 65g Empty Sample Load Cartridges w/Frits, Reverse Phase, 100/pk
Available in small, medium, and large sizes.
SuperFlash Columns
SuperFlash columns are engineered to provide optimal sample loading and flow distribution, resulting in improved resolution and peak performance. All 16 sizes are available with the best silica gel, both flash and MPLC, for improved resolution. SuperFlash columns are compatible with Isco, Biotage and any automated flash system. SuperFlash Columns are available in packs or case quantities. Direct Luer ConnectTM for easy connection to any system without requiring complex compression module hardware. Guaranteed leak proof to 200psi on SF25 and SF40 sizes, and 50psi on the SF65 sizes to preserve your sample. Currently available in 16 standard sizes from 4g to 800g to purify up to 80g of material. Alternative lengths & custom sizes available to meet your specific project needs, less expensive than column stacking. Exclusive dispersion channels promote easier, more even sample loading either by direct injection of dissolved or viscous sample, or by DASi solid sample loading. Made with Gradient Free Compression technology to deliver sharper separation results, less channeling and eliminate split peaks Available with a wide choice of Sorbents: Silica, C18 and Alumina. *Inquire about custom packings.
Cat. #
AX1368-8 (8/pk)* AX1403-8 (8/pk)* AX1369-7 (7/pk)* AX1404-7 (7/pk)* AX1281-6 (6/pk)* AX1212-6 (6/pk)* AX1213-6 (6/pk)* AX1214-6 (6/pk)* AX1215-6 (6/pk)* AX1356-4 (4/pk)* AX1216-4 (4/pk)* AX1217-4 (4/pk)* AX1218-4 (4/pk)* AX1357-3 (3/pk)* AX1219-3 (3/pk)* AX1220-3 (3/pk)*
Picture
Model
SF10-4g SF10-8g SF15-12g SF15-24g SF25-40g SF25-60g SF25-80g SF25-120g SF25-160g SF40-80g SF40-115g SF40-150g SF40-240g SF65-220g SF65-400g SF65-600g
Dimen. DxL
1.3 x 9.5cm 1.3 x 13.5cm 2.03 x 11.2cm 2.03 x 17.4cm 2.67 x 17cm 2.67x 23.5cm 2.67x 29.8cm 2.67x 42.4cm 2.67x 55cm 4.06 x 16.4cm 4.06 x 20.3cm 4.06 x 25.7cm 4.06 x 37.2cm 6.6 x 16.4cm 6.6 x 26.4cm 6.6 x 35.5cm
Flow Rate
18ml/min 18ml/min 30ml/min 30ml/min 40ml/min 40ml/min 40ml/min 40ml/min 40ml/min 85ml/min 85ml/min 85ml/min 85ml/min 100ml/min 100ml/min 100ml/min
Sample Load
40-400mg 80-800mg 120mg-1.2g 240mg-2.4g 340mg-3.4g 600mg-6g 800mg-8g 1.2-12g 1.6-16g 800mg-8g 1.2-11.5g 1.5-15g 2.4-24g 2.2-22g 4-40g 6-60g 5ml 11ml 16ml 32ml 53ml 79ml
Vol.
106ml 158ml 211ml 110ml 152ml 200m 317ml 264ml 519ml 792ml
* Case quantities are available. 55
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EZ Flash System
EZ Flash System (Affordable, Modular Flash System)
EZ Flash is the perfect, compact, low-cost Flash Purification System that enables you to purify from 10mg to 60g of sample in disposable columns. Our EZ Flash cartridges, the SuperFlash purification columns, and DASi sample loading module are designed with standard luer connections. One simple connection enables you to realize the benefits of the EZ Flash Cartridges, DASi and SuperFlash columns on your EZ Flash, Biotage, Isco, or FlashMaster systems. Improve the performance and flexibility of your system by using the adjustable DASi sample loading module and Luer Kit.
5 Easy Steps make EZ Flash ideal for method development and everyday purification:
Connect solvent bottle to EZ Flash Isocratic or Gradient Pump Connect Flash column (Superflash or EZ Flash MT) Condition Column with 2 column volumes of solvent Inject your sample Collect fractions
EZ Flash Pump
Sorbent Technologies Isocratic EZ Flash Pump makes flash chromatography easy, more effective and affordable. EZ Flash Pump is a rugged pulse-free, maintenance-free, variable speed pump that allows for step gradient, is compatible with most organic solvents and is externally controllable with an optional fraction collector. Pressure rated at 40 PSI with Flow range of 10 -140 ml/min. EZ Flash Pump has small foot print: 5.75 W x 11 L x 6 H and is available in 110 & 220V (EU compliant).
EZ Flash Gradient Pump

High Performance
The EZ Flash Gradient Pump is designed to meet the requirements of most flash chromatography applications with high flow rate gradients. Standard features include digital keypad control and RS-232 interface. Flash 150 Binary Pump Features: Two single-head rapid refill pumps for high flow Ceramic pistons and high performance UHMWPE, HPLC grade seals Ruby ball/sapphire seat check valves Universal voltage input: 90260 Vac / 5060 Hz Flow alibration (CAL) can be set for individual solvents Repeatable flows and no loss of prime with Hexane, Methylene Chloride, and other light solvents.
Cat. #
ACCU MP-30 120995 WID2K1012601 EZFC1
Description
EZ Flash Isocratic Magnetic Gear Pump (Carbon Graphite), 40 PSI, Solvent Compatible. EZ Flash Binary Gradient Pump, SS, 200 psi, 0.1-150.0 ml/min SCU470 Gradient Controller Unit, Digital Keypad control, RS-232 Interface, Stores up to 9 Gradients New! EZFC1 Fraction Collector with diverter valve. Collect up to 100 ml/min in 1 rack with a maximum of (144) 13mm tube collection. Includes (1) rack for 13mm tubes. (Substitution of same-price racks must be specified on purchase order.) New! EZFC2 Fraction Collector with diverter valve. Collect up to 100 ml/min in 2 racks with a maximum of (288) 13mm tube collection. Racks with RFID sensors for fast and error free setup. Includes (2) racks for 13mm tubes. (Substitution of same-price racks must be specified on purchase order.) EZ Luer Kit for using male/female luer columns. This kit contains tubing with solvent filter for connecting solvent bottle to pump and tubing for connecting pump to cartridge. Contact closure from Pump to Fraction Collector
EZFC2 EZ LuerKit Misc.
56
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Gradient Flash System Additions

SCU470 Gradient Controller Unit
The compact and versatile SCU 470 Keypad Gradient Controller offers a simple yet effective means of gradient control for the Flash 150 Binary Gradient Pump via RS232 communication. SCU 470 controller has the capability to store 10 gradient methods. To build a gradient enter flow rate and pressure limits. Enter the desired %B, %C, %D values at the different time steps, and the method is automatically saved. The method may be repeated a user-specified number of times, with the trigger for each cycle provided by an external start signal from an injector or autosampler. The status screen displays the current system pressure, current composition, and the stage of the gradient run -- idle mode, initial conditions, waiting for injection, run, delay, etc. A run in progress can be stopped at any time, and a sequence stopped or aborted at any time. Required to control Flash 150 Binary Pump either as a stand alone or with Fraction Collector. Includes 4 pump cables, 1 external start cable, 1 PC cable, SCU control center software CD, 100-240 VAC power supply. Clarity Software allows control of pump, fraction collector and detector with reporting capabilities. Contact us for more information to create a custom automated flash system.
New Generation Fraction Collector

The EZ fraction collector can be adapted to a broad spectrum of applications. The units can distribute fractions into 96 well microplates, standard tube sizes, and bottles. For essentially unlimited volumes, funnel racks can direct fluids to any collection vessel or downstream process.
Standard Features
Collect peaks of interest separately using any combination of slope, level, and time window reliably separates peaks by monitoringdetector output, so you dont have to expend glassware on no-interest effluent. Collect uniform fractions by time, drop count, or pumped volume. Stores up to 8 collection methods Choice of 8 available racks (specify when ordering) Built-in Ethernet and RS-232 communication capabilities Faster tube changes Flow rates to 25 mL/minute
Cat.# EZFC1 Cat.# EZFC2
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57
4-Channel Preparative Flash Chromatography Detector

The 4-Channel Preparative Flash Chromatography Detector is a stand alone flash chromatography detector specifically designed to operate with conventional glass chromatography columns, FPLC chromatography systems, or preparative HPLC columns. Operation with analytical HPLC column is not advised due to the relatively large flow cell volume (150uL). The detector is compatible with any organic or aqueous media excluding hydrofluoric acid. The detector is designed to minimise resistance to solvent flow of up to 300mL/min. This way, even a simple gravity column can be carried out with the 4-Channel Preparative Flash Chromatography Detector.
Two UV channels: 250nm and 280nm 0.001-100 OD range for 1mm optical path 250nm works perfectly with ethylacetate and dichloromethane 10nm bandwidth Deep LED UV sources; no bulbs to change No reference cell, but drift is very low Solvent/Quartz refractive index mismatch compensation Proton mobility Gas bubble tolerant measuring Proton mobility Method developed by REACH Devices Very sensitive for weakly ionic compounds Works in any solvent from water to hexanes Refractive index Range from 1.2000 to 1.7000 or differential against a reference flow cell Measurement at 640nm Good thermal stability Gas bubble tolerant measuring
58
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3-Channel Preparative Flash Chromatography Detector

This unit performs simultaneous three-channel detection: optical density at 250nm and 280nm and proton mobility. The detector connects to usual inexpensive glass flash columns (adaptor included), FPLC and preparative HPLC systems via 1/16 PTFE or PEEK tubing. This unit does not need a computer or other gadgets to operate. Data are shown on a color graphic LCD and saved to internal flash memory. Data can be transferred onto a usual USB flash drive.
Cat. #
EZD3 EZD4 3-Channel Preparative Flash Chromatography Detector 4-Channel Preparative Flash Chromatography Detector
Description
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59
FPLC Columns Econoline - Econoline and Econoline LP

Optimize your purification and chromatography with durable, state-of-the-art, precision engineered Low & Medium Pressure Columns for Semi-Prep and Preparative LPLC, FPLC, to HPLC. These columns allow for easy packing, reproducibility, reliability, and scalability to increase lab productivity.
Econoline
Economical solution for low and medium pressure from 5mm to 50mm diameter and bed length up to 500mm, unique high pressure glass construction (up to 86 bar). Econoline is designed using linear piston motion for superior chromatography. *Replaces XK
Econoline LP
Economical solution for low and medium pressure from 5mm to 50mm diameter and bed length up to 500mm, unique high pressure glass construction (up to 86 bar). Econoline LP is designed using linear piston motion for superior chromatography. *Replaces XK
Column
Econoline
Pressure Limits
30 - 80 Bars
Connections
Two 1/4 - 28 Fittings (one for 1/16 and one for 1/8 capillaries). The column is supplied with an M6 adapter to allow direct connection to any LC system. ID 10mm - 25mm: Two (2) 1/4- 28 fittings for 1/16 capillaries ID 32mm - 50 mm: Two (2) 1/4- 28 fittings for 1/8 capillaries
Height Adjustment
Multi-variable plunger at each end, 70 mm adjustment each.
Accessories
User-friendly packing adapters
Econoline LP
10 - 30 Bars
Multi-variable plunger at one end,120 mm adjustment. Double piston configuration available upon request.
User-friendly packing adapters Frit removal tool
Features and Benefits of Econoline and Econoline LP versus XK Columns

High precision glass with smoother surface eliminates channeling Double piston adjustment available to allow greater flexibility in bed Higher pressure rating allows for wider range of chromatography applications height and adjustment Linear piston eliminates bed compression and shearing effect, allowing better Quick release ends allow for better and easier disassembly of columns sample loading and solvent flow for increased performance Higher precision seal eliminates dead volume True frits for bed stability versus sock design Easy to use packing adapter Inlet and outlet connections located on exterior indicating any possible leaks
Cross-reference tables comparing Econoline to GE Healthcare (formerly Pharmacia) XK & K9 LC Columns.

Sorbtech Cat. #
TAC10/125PE0-AB-1 TAC10/250PE0-AB-1 TAC15/125PE0-AB-1 TAC15/500PE0-AB-1 TAC25/125PE0-AB-1 TAC25/500PE0-AB-1 TAC50/125PE0-AB-1 TAC50/250PE0-AB-1 TAC50/500PE0-AB-1
ID (mm)
10 10 15 15 25 25 50 50 50
Bed Height (mm)

0 - 125 110 - 250 0 - 125 360 - 500 0 - 125 360 - 500 0 - 125 110 - 250 360 - 500
Volume (mL)
0 - 9.82 8.64 - 19.63 0 - 22.09 63.62 - 88.36 0 - 61.36 176.71 - 245.44 0 - 245.44 215.98 - 490.87 706.86 - 981.75
GE Cat. #
*K9/15 *K9/30 XK 16/20 XK 16/40 XK 26/20 XK 26/40 XK 50/20 XK 50/30 XK 50/60 9 9
ID (mm)
Bed Height (mm)

140 290 25 - 150 225 - 350 10 - 125 230 - 350 0 - 140 135 - 285 405 - 555 9
Volume (mL)
18 5 - 31 45 - 70 5.3 - 66 122 -186 0 - 274 265 - 559 794 - 1088
16 16 26 26 50 50 50
Note: Frit Porosity is available in 2m & 10m
Note: *GE K9 Columns are not available with variable piston
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FPLC Columns - Upscale - IsoKrom

Upscale
Small and mid size low pressure pilot / process columns from 90mm to 450mm diameter with bed lengths up to 900mm. Upscale offers a glass column solution with superior flow distribution as well as a positive (no dead volume) mechanical seal. Design eliminates metal contact with wetted parts.
HIGHLIGHTS:
Simple Sanitary Design Low Dead Volume Reliable Mechanical Seal Low Maintenance Scalable Replaces BPG
Cross-reference tabels comparing Upscale to GE Healthcare (formerly Pharmacia) LC Columns.

Sorbtech Cat. #
265111 265117 265118 NA NA NA 265121 265127 265128 265131 265137 265138 265141 265147 265148
ID (mm)
90 90 90 NA NA NA 180 180 180 300 300 300 450 450 450
Bed Height (mm)

0 - 25 15 - 55 45 - 85 NA NA NA 0 - 25 15 - 55 45 - 85 0 - 21 11 - 51 41 - 81 0 - 21 11 - 51 41 - 81
Volume (L)
0 - 1.6 0.9 - 3.5 2.9 - 5.4 NA NA NA 0 - 6.4 3.8 - 14 3.8 - 14 11.5 - 21.6 0 - 14.8 28.9 - 56.2 0 - 33.4 17.1 - 79.3 63.7 - 128.8
Pressure Rating (bar)

6 6 6 NA NA NA 4 4 4 3 3 3 2 2 2
GE Cat. #
BPG 100/500 BPG 100/750 BPG 100/950 BPG 140/500 BPG 140/750 BPG 140/950 BPG 200/500 BPG 200/750 BPG 200/950 BPG 300/500 BPG 300/750 BPG 300/950 BPG 450/500 BPG 450/750 BPG 450/1000
ID (mm)
100 100 100 140 140 140 200 200 200 296 296 296 446 446 446
Bed Height (mm)

0 - 26 25 - 41 45 - 54 0 - 26 25 - 41 45 - 54 0 - 26 25 - 41 45 - 54 0 - 26 25 - 41 45 - 54 3 - 23 22 - 39 47 - 58
Volume (L)
0-2 2 - 3.2 3.5 - 4.2 0-4 3.9 - 6.3 6.9 - 8.3 0 - 8.2 7.3 - 12.9 14.1 - 16.9 0 - 17.9 17.2 - 28.2 31 - 37.2 4.7 - 35.9 34.4 - 60.9 73.4 - 90.6
Pressure Rating (bar)

8 8 8 6 6 6 6 6 6 4 4 4 2.5 2.5 2.5
Note: Frit Porosity is 20m for UpScale and 23m for GE
IsoKrom Series
These low and medium pressure fixed bed, side packed columns offer zero dead volume technology. Sizes are available from 95mm to 2,000mm with no practical limit on bed length. Simple construction uses standard components, providing low back-end costs of ownership.
HIGHLIGHTS:
All wetted parts are either autoclavable, disposable and/or can be completely taken apart for thorough cleaning. Simple & Robust - IsoKrom is designed around standard industrial parts, to provide a lower price and higher quality. Reduced dead-space by volume (V) and length (L). Fixed volume, packing through the wall. No metal parts contact the solution.
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61
Syringe Filters
Chromafil Disposable Syringe Filters - Quick Filtration of Liquid Samples
CHROMAFILTM Disposable Syringe Filters are used for filtration of suspended matter from liquid samples. They feature:
Polypropylene Housing
Better solvent stability compared to acrylate and polystrene filters
Shells ultrasonically sealed, not glued

No extractable components from glues
Filtration in both directions possible

The liquid cannot bypass the membranes.
Luer lock on side of entry

Safe connection on thehigh pressure side
Luer exit
Standard luer for 3 mm and 25 mm filters, minispike luer with low dead volume and small OD for 15 mm filters
Deflector
The stream of liquid is broken and distributed, and does not directly hit the membrane: this prevents rupture of the membrane
Star-shaped distribution device

The liquid is evenly distributed to the whole membrane surface: This results in a better utilization of the total area. The filter is not plugged up rapidly, providing high flow efficiency. CHROMAFILTM filters are available with pore sizes of 0.2m to 1.0m and in the following filter membranes: Cellulose Mixed Ester (MV) Cellulose Acetate (CA) Regenerated Cellulose (RC) Polyamide (PA) Nylon Teflon (PTFE) Polyester (PET) Glass Fiber (GF) Glass Fiber (GF) / Polyester (PET)
Color Coded
Filter with 0.2m pores have a yellow upper shell and filters with 0.45m pores are colorless. The different membrane types are distinguished by different colors.
Low Dead Volume

~120l for 25 mm , 12l for 15 mm , 5l for 3 mm With CHROMAFILTM, rapid purification and removal of particles from liquid or gas samples is simple: place the filter on the syringe and you are ready for filtration. Special manipulations are not required. Contamination of sensitive instrumentation by solid impurities can be avoided, thus increasing the lifetime of chromatographic columns and equipment.
(MV)
(CA)
(RC)
(PA)
(PTFE)
(PET)
(GF)
(GF/PET)
Inquire about bulk packs.
Cat. #
729004 729027 729031 729013 729205 729019 729020 729028 729033
TM TM
Description
ChromafilTM Cellulose Mixed Ester Chromafil Cellulose Acetate Chromafil Regenerated Cellulose ChromafilTM Polyamide Nylon Chromafil Teflon
TM TM
Pore Size
0.45m 0.45m 0.45m 0.45m 0.45m 0.45m 0.45m 1.0m 0.45m
Diameter
25mm 25mm 25mm 25mm 25mm 25mm 25mm 25mm 25mm
Color Top/Bottom
Colorless/Yellow Colorless/Red Colorless/Blue Colorless/Green Colorless/Colorless White/White Colorless/Orange Yellow/Black Black/Orange MV CA RC PA
Material
Qty/Pkg
100 100 100 100 100 100 100 100 100
PTFE PVDF PET GF GF/PET
Chromafil Polyvinylidene Difluoride ChromafilTM Polyester Chromafil Glass Fiber

TM TM
Chromafil Glass Fiber / Polyester
For more selections and sizes, visit our website or contact your personal Product Specialist 62 Sorbent Technologies, Inc. Toll Free: 866.SORBTEC Fax: 770.936.0326 www.sorbtech.com
HPLC Columns
Sorbtech Purity - HPLC Columns
A Sorbent Technologies Exclusive
Sorbent Technologies introduces Purity the next generation of analytical and prep columns for small and large molecules (proteins & peptides) to maximize purification and purity of compounds. Purity is packed with SorbTechs Premium Pure Silica and uses a unique packing method to guarantee the best performance: resolution, reproducibility, and lifetime. Custom bonding and packing
Purity HPLC is available in analytical, semi-prep, prep and custom sizes.
Bonded Phases: C4, C8, C18, C18AQ, C18DE, Amino, Phenyl, Cyano, Diol, & Custom Particle Sizes: 2, 3, 5, & 10m Pore Sizes: 100, 150, 200, 300 & 500 Analytical: 2.0 to 4.6mm ID, 50 to 250mm L Prep: 10 to 100mm ID, 50 to 250mm L Let Purity optimize your method development and scale up from analytical to prep to process. Selectivity, Speed, Optimization, Loadibility, Lifetime, Resolution, Reproducibilty, PurityCustom Packing We offer custom packing of Purity columns with Sorbtech adsorbents from analytical to preparative. Additionally, we can pack other manufacturers resins from analytical to preparative.
C18 AQ, 300, 10m
Mobile Phase: 60/40 Acetonitrile/Water Flow Rate: 1.0 mL/min Pressure: 375 psig Column Dimensions: 250 x 4.6 mm Detection: 254 nm Sample Size: 10 L
Cat. #
10263030-4.6x250 10463030-4.6x250 10483030-4.6x250 10155030-4.6x250 10265030-4.6x250 10465030-4.6x250
Description
Premium Pure C8 Silica Gel Premium Pure C18 Silica Gel Premium Pure C18AQ Silica Gel Premium Pure C4 Silica Gel Premium Pure C8 Silica Gel Premium Pure C18 Silica Gel
Particle Size
5m 5m 5m 5m 5m 5m 100 100 100 300 300 300
Pore Size
ID x L
4.6 x 250mm 4.6 x 250mm 4.6 x 250mm 4.6 x 250mm 4.6 x 250mm 4.6 x 250mm
Available in a wide selection of phases and sizes: analytical and preparative For more selections and sizes, visit our website or contact your personal Product Specialist Sorbent Technologies, Inc. Toll Free: 866.SORBTEC Fax: 770.936.0326 www.sorbtech.com 63
HPLC Columns - Grace Vydac

Specializing in Protein Purification
Sorbent Technologies offers the complete line of Grace Vydac HPLC columns. Vydac set the standard in 300 Columns for the separation of proteins, peptides, and oligonucleotides with the TP line of Polymeric bonded end-capped columns in C4, C8, and C18 phases. The Everest (300) and Denali (120) are their next generation of high performance columns. Columns are available in Analytical, Semi-prep, Prep, and LC-MS from 3m to 20m.
Column Selection for Polypeptides
Vydac TP - Series Columns

Industry Standard for Polypeptide Separations Referred in over 9000 patents, Vydac 300 TP is the industry-standard, for peptide, protein, and large molecule separations Polymeric bonded phases have exceptionally long column lifetime and negligible phase leaching Extensive applications library based on over two decades experience Vydac TP reversed-phase material consists of aliphatic groups bonded to the surface of 300 pore diameter silica. The large pores of the 300 TP silica give polypeptide molecules complete access to the interior of the silica pores. The unique process by which Vydac TP silica is manufactured results in a high purity, synthetic silica with carefully controlled characteristics. Vydac TP silica is the standard that has defined large pore HPLC silica for polypeptide separations for nearly two decades.Vydac 214TP C4 Vydac 214TP is a polymerically bonded end-capped n-butyl reversed-phase based on 300 TP silica. The 214ATP column has been found more suitable for resolution of degradation products in analysis of biosynthetic human growth hormone.
Insulin Variants
Applications Glycoproteins Hemoglobin variants Histones Human growth hormone Insulin variants Membrane proteins
Column: Vydac 214TP54 Gradient: 27-30% ACN with 0.1% TFA over 25 min
Cat. #
214TP5205 214TP5405 214TP52 214TP54 214TP104 214TP1022
Description
C4 Polymeric RP C4 Polymeric RP C4 Polymeric RP C4 Polymeric RP C4 Polymeric RP C4 Semi-Prep Polymeric RP
Particle Size
5m 5m 5m 5m 7m 10m 300 300 300 300 300 300
Pore Size
2.1 x 50mm 4.6 x 50mm
ID x L
2.1 x 250mm 4.6 x 250mm 4.6 x 250mm 22 x 250mm
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Vydac 208TP C8 Reversed-Phase

Vydac 208TP is a polymerically bonded end-capped n-octyl reversedphase based on 300 TP silica. Applications: Vydac 208TP reversed-phase columns are recommended for the separation of: Polypeptides up to 10,000-20,000 MW Enzymatic digest fragments Natural and synthetic peptides
Separation of Tryptic Digest
Column: Vydac 208TP54 Flow: 1.0 mL/min Gradient: 0-30% ACN with 0.1% aqueous TFA over 60 min Sample: Tryptic digest of -lactoglobulin A
Cat. #
208TP5415 208TP54 208TP510 208TP1022
Description
C8 Polymeric RP C8 Polymeric RP C8 Polymeric RP C8 Semi-Prep Polymeric RP
Particle Size
5m 5m 5m 10m 300 300 300 300
Pore Size
ID x L
4.6 x 150mm 4.6 x 250mm 10 x 250mm 22 x 250mm
Human Apolipoproteins
Vydac 218TP C18

Vydac 218TP is a polymerically bonded end-capped n-octadecyll reversedphase based on 300 TP silica. Applications: Vydac 218TP reversed-phase columns are recommended for the separation of: Small polypeptides less than 4,000-5,000 MW Enzymatic digest fragments Natural and synthetic peptides Multi-ring compounds Specific examples include: Tryptic digests S. aureus V8 digests Natural and synthetic peptides Peptide studies
Column: Vydac 218TP54 Flow: 1.2 mL/min Column Temp: 50C Mobile Phase: A: 25% ACN/0.1% TFA in water B: 58% ACN/0.1% TFA in water Gradient: 0-100% B in 33 min Peaks: 1. apoC-llla; 2. apoC-lllb; 3. apoC-lla; 4. apoC-lllc; 5. apoC-l; 6. apoC-llb; 7. apoA-la; 8. apoA-lb; 9. apoA-llc; 10. apoA-lla; 11. apoA-llb The standard was human insulin
Cat. #
218TP5215 218TP5415 218TP52 218TP54 218TP510 218TP1010
Description
C18 Polymeric RP C18 Polymeric RP C18 Polymeric RP C18 Polymeric RP C18 Polymeric RP C18 Semi-Prep Polymeric RP
Particle Size
5m 5m 5m 5m 5m 10m 300 300 300 300 300 300
Pore Size
ID x L
2.1 x 150mm 4.6 x 150mm 2.1 x 250mm 4.6 x 250mm 10 x 250mm 10 x 250mm
HPLC Columns - Macherey-Nagel

Macherey-NagelTM is a world leader in chromatography products. Macherey-NagelTM, known for industry standard Nucleosil, introduces their new ultra-high purity Nucleodur products for HPLC. Nulceodur offers increased separation, reproducibility, reliability, and scalability. Now available in 1.8m for ultra-fast HPLC.
Macherey-NagelTM Nucleodur
Nucleodur is the new generation of totally spherical, high purity silica gels. These gels offer the best in separation performance and are now available in 1.8m for ultra-fast HPLC. Gravity, Isis, Pyramid, and Sphinx have unique bonding characteristics providing solutions for most separation applications. Ultra-High Purity High pressure stability Maximum Bed & pH Stability Maximize Efficiency High Separation Efficiency High Mechanical and chemical stability Increased load capacity Ideal particle shape and surface symmetry Reproducibility from lot to lot and column to column
1.8m Particle Size for Ultra-Fast HPLC

Nucleodur is now available in 1.8m for Ultra-Fast HPLC to allow extraordinary improvement in plate numbers, column efficiencies, and resolution compared to 3m. Decrease of analysis time (ultra fast HPLC) Shorter columns with high separation efficiency Significant improvement of resolution Increased detection sensitivity All NUCLEODUR premium phases are available in 1.8m: C18 Gravity, C8 Gravity, C18 Isis, C18 Pyramid, Sphinx RP NUCLEODUR 1.8m particles are fractionated to limit the increase in back pressure
Features of 1.8m NUCLEODUR silica particles

Increase of separation efficiency by higher number of theoretical plates (N):
50 x 4.6 mm NUCLEODUR C18 Gravity 3 m: N 100,000 plates/m (h value 10) 1.8 m: N 166,667 plates/m (h value 6) Increase of the plate number by app. 67% offers the possibility of using shorter columns with equal plate numbers resulting in a decrease of analysis time.
Significant improvement in resolution

Use of 1.8m instead of 3m particles leads to an increase of resolution by a factor 1.29 (29%) since the resolution is inversely proportional to the square root of the particle size.
Column back pressure

Because of the high sphericity of the NUCLEODUR particles and the very narrow particle size distribution we are able to keep the back pressure on a moderate level.
Resolution as a function of particle size

Column: Eluent: Flow rate: Pressure: Detection: Peaks: 1. Naphthalene 2. Ethylbenzene 50 x 4 mm NUCLEODUR C18 Gravity A) 3m, B) 1.8m acetonitrile water (80:20, v/v) 2 ml/min A) 80 bar, B) 160 bar UV, 254 nm
Higher flow rates and shorter run times

Optimal flow rate for 1.8m particles is higher for 3m and 5m particles (the flow rate should be at the van-Deemter minimum).
Van-Deemter plot
column 50 x 4.6 mm, acetonitrile water (50:50, v/v), analyte toluene
25
5 m 3 m 1.8 m
0.0 0.5 1.0 1.5 2.0 2.5 3.0 3.5 4.0
HETP [m]
20 15 10 5 0
u [mm/s]
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Nucleodur C18 Gravity C8 Gravity

Nucleodur Gravity columns are designed to handle high pH (up to 11), and can replace WatersTM XterraTM, PhenomenexTM LunaTM, ZorbaxTM ExtendTM and other high end columns for pharmaceutical applications such as analgesics, anti-inflammatories, anti-depressants, herbacides, phytopharmaceuticals, immunosuppressants, etc. Preparative columns available by contacting your personal Product Specialist.
Features
Available as octadecyl (C18 USP L1) and octyl (C8 USP L7) modification Polymeric bonded phases have exceptionally long column lifetime and negligible phase leaching Pore size 110 ; particle sizes 1.8 m, 3 m and 5 m for C18, 1.8 and 5m for C8 7, 10, 12 and 16 m particles for preparative purposes are available on request Ideal for method development Allows HPLC at pH extremes (pH 1 11) Suitable for LC/MS due to low bleeding characteristics Recommended for overall sophisticated analytical separations Compound classes separated so far: pharmaceuticals, e. g. analgesics, anti-inflammatory drugs, antidepressants; herbicides; phytopharmaceuticals; immunosuppressants
Surface Silanols at different pH values
The figure above shows the extent of protonation of surface silanols and of two exemplary analytes at acidic and alkaline pH.
Comparison of different base deactivated phases
Separation of Pyridine and Phenol

1
Phase S (C18, 5 m) Phase P (C18, 5 m) Phase L (C18, 5 m)

1 and 2 overlap
3 1 2
Peaks: 1. Pyridine 2. Phenol
Phase I (C18, 5 m) NUCLEODUR C18 Gravity, 5 m
10
Columns: Eluent:
250 x 4 mm methanol 20 mM KH2PO4, pH 7.0 (75 : 25, v/v) Flow rate: 1.0 ml/min Temperature: 30 C Detection: UV, 254 nm
15
20
25 min
Peaks: 1. Dibutyl phthalate 2. Acenaphthene 3. Amitriptyline
10
min
Column: Eluent: Flow rate: Temperature:
250 x 4 mm NUCLEODUR C18 Gravity, 5 m methanol water (30 : 70, v/v) 1.0 ml/min Detection: UV, 254 nm 40 C Inj. volume: 5 l
Comparison of different base deactivated phases
Separation of pyridine and phenol
Cat. #
760079.46 760101.46 760753.46
Description
Nucleodur C18 Gravity Nucleodur C18 Gravity Nucleodur C8 Gravity
4 Particle Size
1.8m 5m 5m
Pore Size
110 110 110
ID x L
4.6 x 50mm 4.6 x 250mm 4.6 x 250mm

Nucleodur C18 Isis
Nucleodur C18 Isis offers high steric selectivity and unique surface modifications for steroids, aromatics and fat soluble vitamins. Isis replaces InertsilTM ODS-P & YMC Pro C18RS.
Features
C18 phase with special polymeric, crosslinked surface modification USP L Pore size 110; particle sizes 1.8m, 3m and 5m; 20% C Exceptional steric selectivity Outstanding surface deactivation Suitable for LC/MS due to low bleeding characteristics pH stability 1 10 Broad range of applications: steroids, (o,p,m-) substituted aromatics, fat-soluble vitamins
Cat. #
760405.46 760414.46
Description
Nucleodor C18 Isis Nucleodor C18 Isis
Particle Size
1.8m 5m
Pore Size
110 110
ID x L
4.6 x 50mm 4.6 x 250mm
Nucleodur C18 Pyramid

Nucleodur C18 Pyramid is stable in 100% aqueous and designed for analgesics, penicillin antibiotics, nucleic acid bases, and more. Pyramid replaces PhenomenexTM AquaTM, YMC AQTM, & WatersTM AtlantisTM dC18.
Separation of very polar compounds

1 2
Features
Stable in 100% aqueous mobile phase systems USP L1 Pore size 110; particle sizes 1.8m, 3m and 5m; 14% C 7 and 10m particles for preparative purposes are available on request Interesting polar selectivity features Excellent base deactivation; suitable for LC/MS due to low bleeding characteristics pH stability 1 9 Classes of compounds separated so far: analgesics, penicillin antibiotics, nucleic Acid bases, water-soluble vitamins, complexing agents, organic acids
Detection: UV, 202 nm Peaks: Peaks: (injection 22 l) (injectionvolume volume l) 1. Formic acid 1. Formic acid 2. Acetic acid 2. Acetic acid 2 4 min 0 Fig. 14: Separation of very polar compounds
Column: NUCLEODUR C18 Pyramid, 5 m, 125 x 4mm C Column: NUCLEODUR 18 0.2% H PO Eluent: 3 4 Pyramid, 5 m, 125 x 4 mm Flow rate: Eluent: 0.2% H3PO4 1.0ml/min Temperature: 22C 0 Flow rate: 1.0 ml/min Detection: UV, 202 nm Temperature: 22 C
Column: NUC Pyramid, 5 m Eluent: Flow rate: Temperature: Detection:
Fig. 15: Sepa
Separation of organic acids

1 3 2 NUCLEODUR C18 Pyramid 4 3
Column: NUCLEODUR C18 Pyramid, 5 m, 250 x 4mm Eluent: 0.2% H3PO4 Flow rate: 0.7ml/min Temperature: 25C Detection: UV, 210 nm conventional RP colum Peaks: (injection volume 2l) 1. Tararic acid 2. Malic acid 3. Lactic acid initial 1 4. Succinic acid injection
Column: NUCLEODUR C18 2 Pyramid, 5 m, 125 x 4 mm Eluent: 0.2% H3PO4 1 0 5 10 min Flow rate: 1.0 ml/min 2 Temperature: 22 C Cat. # Description Particle Size Size ID x Column: NUCLEODUR C18 Pore Peaks: Detection: UV, 202 nm Pyramid, 5 m, 250 x 4 mm (injection volume 2 l) 760272.46 Nucleodor C18 Peaks: Pyramid 1.8m 110 1. Tartaric acid 4.6 x 50mm Eluent: 0.2% H3PO4 (injection volume 2 l) Flow rate: 0.7 ml/min 2. Malic acid 760202.46 Nucleodor C18 1. Pyramid 5m 110 4.6 x 250mm Formic acid 0 5 min3. Lactic acid 0 Temperature: 25 C 2. Acetic acid Detection: UV, 210 nm 4. Succinic acid pump 68 2 4 min 0 stopped! Sorbent Technologies, Inc. Toll Free: 866.SORBTEC Fax: 770.936.0326 Fig. 15: Separation of organic acidswww.sorbtech.com Fig. 14: Separation of very polar compounds 3
5 mi
Nucleodur Sphinx RP
Nucleodur Sphinx RP has distinct selectivity based on bifunctional surface coverage that focuses on quinolone antibiotics, sulfonamides, xanthines, and substituted aromatics. Sphinx is unique and stands alone.
Features
Distinct selectivity based on bifunctional surface coverage USP L1 and USP L11 Pore size 110; particle sizes 1.8m, 3m and 5m; 14% C High density of covalently bonded silanes for tailing-free peaks Widens the scope for method development pH stability 1 10 Suitable for LC/MS due to low bleeding characteristics High reproducibility and consistent quality due to tight QC procedures Range of applications: quinolone antibiotics, sulfonamides, xanthines, substituted aromatics
Stability of NUCLEODUR Sphinx RP at pH 10

Column: Eluent: Flow rate: Detection: Injection volume: 50 x 4.6 mm NUCLEODUR Sphinx RP, 5 m methanol dil. NH3, pH 10 (20:80, v/v) 1.0 ml/min, temperature 30 C UV, 275 nm 3 l Peaks: 1. Theophylline 2. Caffeine
Cat. #
760821.20 760822.46 760807.30 760808.46 760800.46 760801.40 760802.46 760803.46
Description
Nucleodur Sphinx RP Bifunctional Nucleodur Sphinx RP Bifunctional Nucleodur Sphinx RP Bifunctional Nucleodur Sphinx RP Bifunctional Nucleodur Sphinx RP Bifunctional Nucleodur Sphinx RP Bifunctional Nucleodur Sphinx RP Bifunctional Nucleodur Sphinx RP Bifunctional
Particle Size
1.8m 1.8m 3m 3m 5m 5m 5m 5m
Pore Size
110 110 110 110 110 110 110 110
ID x L
2 x 30mm 4.6 x 50mm 3 x 125mm 4.6 x 250mm 4.6 x 50mm 4 x 125mm 4.6 x 150mm 4.6 x 250mm

NUCLEODUR C18 ec / C8 ec
Nucleodur C18 ec and C8 ec is ideal for daily routine reverse phase analysis and scale up to Prep. Exceptional silane bonding and endcapping allows for tailing-free elution of polar compounds, optimal chemical stability and pH stable from 1-9. These Nucleodur products replace Spherisorb, Hypersil, Waters Symmetry, Kromasil, & LiChrospher
Features
Medium density octadecyl (USP L1) and octyl phases (USP L7) Pore size 110; particle sizes 3m and 5m; 7m, 10m, 12m, 16m, 20m, 30m and 50m for preparative separations For daily routine analysis and up-scaling for preparative HPLC pH stability 1 9 High reproducibility from lot to lot For standard routine reversed phase applications
pH stability of NUCLEODUR C18 ec

Separation of theophylline and caffeine at pH 10
Column: 30 x 3 mm NUCLEODUR 100-5 C18 ec Mobile phase: methanol aq. NH3 (20:80, v/v), pH 10 Flow rate: 0.5ml/min Temperature: 25C Detection: UV254 nm
Cat. #
760002.46 760703.46
Description
Nucleodur C18 ec Nucleodur C8 ec
Particle Size
5m 5m 110 110
Pore Size
ID x L
4.6 x 250mm 4.6 x 250mm
NUCLEODUR HILIC
High purity silica phase for Hydrophilic Interaction Liquid Chromatography Features Ideal for reproducible and stable chromatography of highly polar analytes Suitable for analytical and preparative applications as well as LC-MS Very short column conditioning period Technical characteristics: ammonium sulphonic acid modified silica: pore size 110 , available particle sizes 1.8 m, 3 m and 5 m; carbon content 7 %; pH stability 2 8.5 Recommended application: hydrophilic compounds such as organic polar acids and bases, polar natural compounds, nucleosides, oligonucleotides, amino acids, peptides, water soluble vitamins
Separation of Organic Acids on NUCLEODUR HILIC
Separation of Organic Acids on NUCLEODUR HILIC Substances: fumaric acid; oxalic acid; citric acid Column: NUCLEODUR HILIC, 110, 3 m, 4 x 125mm Phase: NUCLEODUR HILIC Cat. #: 760531.40 Conditions: Eluent: acetonitrile / 200 mM ammonium acetate, pH 6.8 (70:30, v/v) Flow rate: 1.0 ml/min Temperature: 25 C Injection volume: 0.5 l Detection: UV, 220 nm
Cat. #
760523.20 760526.20 760531.46 760530.46 760551.46 760550.46
Description
Nucleodor Hilic Nucleodor Hilic Nucleodor Hilic Nucleodor Hilic Nucleodor Hilic Nucleodor Hilic
Particle Size
1.8m 1.8m 3m 3m 5m 5m 110 110 110 110 110 110
Pore Size
ID x L
2 x 50mm 2 x 100mm 4.6 x 125mm 4.6 x 250mm 4.6 x 125mm 4.6 x 250mm
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Nucleodur CN / CN-RP
Nucleodur is available in CN for normal phase and reverse phase CN-RP, widening the scope of selectivity. The cyano phase is stable against hydrolysis at low pH with a working range of pH 1-8. CN-RP is designed for polar organic compounds such as basic drugs, tricyclic anti-depressants, steroids, organic acids, and molecules with electron systems.
Features
Pore size 110; particle sizes 3m and 5m; 7% C USP L10 Multi-mode columns (RP and NP) Widens the scope in selectivity Different retention characteristics in comparison to C8 and C18 Stable against hydrolysis at low pH, working range pH 1 8 High reproducibility from lot to lot Classes of compounds separated so far: tricyclic antidepressants, steroids, organic acids
Reproducibility of NUCLEODUR CN-RP

Column: 250 x 4 mm NUCLEODUR 100-5 CN-RP Eluent: acetonitrile water (60:40, v/v) Flow rate: 1.0 ml/min, temperature 20C Detection: UV254 nm, injection volume: 5l
Peaks: 1. Benzamide 2. Dimethyl phthalate 3. Phenetole 4. o-Xylene 5. Biphenyl
Cat. #
760151.46 760150.46 760156.46 760153.46 760152.46 Nucleodur CN Nucleodur CN
Description
Particle Size
5m 5m 3m 5m 5m 110 110 110 110 110
Pore Size
ID x L
4.6 x 125mm 4.6 x 250mm 4.6 x 150mm 4.6 x 125mm 4.6 x 250mm
Nucleodur CN-RP Nucleodur CN-RP Nucleodur CN-RP
NUCLEODUR NH2 / NH2-RP

High purity silica phase for HPLC
Features
Multi-mode columns (for RP, NP and IC) Stable against hydrolysis at low pH (working range pH 2 8), 100 % stable in water; suitable for LC/MS Widens scope of analytical HPLC into the polar range Technical characteristics: aminopropyl-modified high purity silica; pore size 110 , particles sizes 3, 5 and 7 m; 2.5 % C; not end-capped Recommended application: Polar compounds under RP conditions (sugars, DNA bases), hydrocarbons under NP conditions Normal phase chromatography (NP) with hexane, dichloromethane or 2-propanol as mobile phase for polar compounds such as substituted anilines, esters, chlorinated pesticides Reversed phase chromatography (RP) of polar compounds in aqueous-organic eluent systems Ion exchange chromatography of anions and organic acids using conventional buffers and organic modifiers
Cat. #
760722.46 760742.46 760732.46
Description
Nucleodor NH2 Nucleodor NH2-RP Nucleodor NH2-RP
Particle Size
5m 3m 5m 110 110 110
Pore Size
ID x L
4.6 x 250 mm 4.6 x 150 mm 4.6 x 250 mm
Nucleosil
The standard in HPLC is a family of completely porous spherical silicas that offers optimized bonding phases for every application. An incredible selection of RP phases that include Nautilus for 100% aqueous applications, as well as, C18, C8, C4, C2, Amino, Phenyl, Cyano, Nitro, Diol, Dimethylamino, Sulphonic Acid, Quaternary Ammonium, SAX, SCX, Chiral, and more. Packing sizes from 3m to 10m and pore sizes from 100 to 4000. Available in analytical, semi-prep, and prep columns. Custom columns are also available.
Features
High bed stability High efficiency High separation performance High mechanical and chemical stability High load capacity Reproducibility from lot to lot and column to column
Cat. #
720014.40 720041.46 720052.46 720059.46 720095.46 720205.46 Nucleosil C18 Nucleosil C18 Nucleosil C8 Nucleosil C4
Description
Particle Size
5m 5m 5m 5m 5m 5m 100 120 120 300 100 100
Pore Size
ID x L
4 x 250mm 4.6 x 250mm 4.6 x 250mm 4.6 x 250mm 4.6 x 250mm 4.6 x 250mm
Nucleosil NH2 (Amino) Nucleosil CN-RP (Cyano)
Analysis of nitroaromatic compounds (explosives)
Determination of Polyamines in Waste Water
Column: ChromCart, NUCLEOSIL C18, 120, 3m, 4 x 125mm Phase: NUCLEOSIL C18, 120, 3m Catalog #: 721666.30 Substances: Diamino-4-nitrotoluene-26 diamino-4-nitrotoluene-24 hexogene hexahydro-135-trinitro-135-triazine trinitrobenzene-135 methyl3-nitroaniline-2 methyl-5-nitroaniline-2 methyl-3-nitroaniline-4 dinitrobenzene-13 trinitrotoluene-246 amino-26-dinitrotoluene-4 am. Conditions: Eluents: A) Methanol B) Water Gradient: 35% A in 12 min. to 55% A, isocratic till 52 min., then 100 % A Temperature: 30 C Injection vol.: 40l Flow rate: 0.35 ml/min.
Column: NUCLEOSIL C18, 100, 5m, 4 x 250mm Phase: NUCLEOSIL C18, 100, 5m Catalog #: 720014.40 Substances: hydroxyputrescine diaminopropane putrescine cadaverine diaminooctane spermidine sym-homospermidine homospermidine Information: Derivatisation with dansylchlorid Conditions: Eluent: A: acetonitrile B: water Gradient: 60 to 15% B in 35min. Temperature: 40C Flow rate: 1.0 ml/min Detection: Fluorescense
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HPLC / Polymer - MCI Gel

MCI Gel Highly Stable Polymeric Analytical Columns
We offer MCI GEL columns with reversed phase, size exclusion, ion exchange and hydrophobic polymer based packings for proteins, oligosaccarides, peptides, amino acids and other applications. The use of polymeric based columns has become more widespread thanks to unique selectivity of the polymer matrix that has no specific adsorption common with silica based packings. These columns can be operated with a wide pH rangeboth basic and acidic eluentsdue to the chemical stability of the inert polymeric materials. Columns are available in analytical, semi-prep and prep sizes. MCI GEL reversed phase columns are based on a polystyrenic and polymethacrylate porous polymers. These columns are normally applied to the separation of aromatic and aliphatic based compounds in the isocratic and gradient elution modes. The applications include pharmaceuticals, steroids, small peptides, amphoteric molecules such as sulfonamides and cephalosporin antibiotics, plus basic drugs, simple amines, antihistamines and carbamate pesticides. Excellent separation performance Chemically stable Wide pH range (very strong acids & bases) Long Lifetime, easily cleaned No irreversible binding associated with silica based columns Accurate results
Separation of proteins mixture
Reversed phase resins CHP10M and CHP5C are recommended for analysis of more hydrophobic substances, and CHP2MG and CHP2MGM are recommended for hydrophilic samples. Cat. #
040101 040201 020401 020201
Description
MCI Gel CHP-10M Polystyrene RP MCI Gel CHP2MGM Polymethacrylate RP MCI Gel CHP5C Polystyrene RP MCI Gel CHP2MG Polymethacrylate RP
Particle Size
4m 4m 10m 10m
ID x L
4.6 x 150mm 4.6 x 150mm 4.6 x 250mm 4.6 x 250mm 1 - 13 2 - 12 1 - 13 2 - 12
pH
CK & CA are cation & anion exchange columns for food applications for sugars and oligosaccarides. Cat. #
001001 011101
Description
MCI Gel CK08E Cation MCI Gel CA08F Anion
Particle Size
9m 7m
ID x L
8 x 300mm 4.6 x 250mm
Counter Ion
Na+ Cl-
SCK & SA are surface functionalized resins for non-suppressed ion chromatography applications. Cat. #
003404 013302
Description
MCI Gel SCK01 Cation MCI Gel SCA04 Anion
Particle Size
9m 5m 4.6 x 150mm 4.6 x 150mm
ID x L
CQP are designed for size exclusion chromatography. Cat. #

021401 MCI Gel CQP10
Description
Particle Size
10m
ID x L
7.5 x 600mm
Pore Size
200
HPLC / Polymer - MCI Gel

Seperation of Phthalic acid esters Amines
Separation of proteins mixture Standard anions
ProtEx Series
MCI GEL ProtEx series packed columns are for ion exchange chromatography which separate sample proteins mainly via ionic interaction between packing material and sample molecules. The packing materials for ProtEx series columns are based on 5m, mono disperse, porous type, methacrylate polymer, and are specifically designed for separation of proteins. On a conventional protein separation column, non-specific adsorption of sample proteins sometimes occurs resulting in loss of valuable sample. But on the ProtEx columns, non-specific adsorption is eliminated because the surface of the packing material is surrounded by a hydrophilic layer which is chemically bonded to the base material, and therefore, ion exchange of funtional groups is increased.
ProtEx series are for ion exchange for sample proteins. Cat. #
014604 003704 MCI Gel ProtEx-SP
Description
MCI Gel ProtEx-DEAE
Particle Size
5m 5m
ID x L
7.5 x 100mm 7.5 x 100mm 2 - 12 1 - 13
pH
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MCI Gel Chiral Separation Columns

MCI GEL CRS10W and its companion product MCI GEL CRS15W (an optical isomer of CRS10W) are based on a 3m with 100 mean pore diameter of silica gel coated with N, N-Dioctyl -L(or D)-alanine which is a novel optical activated ligand. The chiral resolution mechanism is a combination of ligand exchange and hydrophobic interaction. A copper sulfate aqueous solution is used as an eluent. Elution samples are directly detected at a wave length of 254nm because the complex compound , composed of the sample molecule and copper in the eulent, are the objects of detection. With the CRS10W, D-isomers generally elute in front of L-isomers while L-isomers elute ahead of D-isomers on the CRS15W. The hydrophobic interaction mechanism allows hydrophilic samples to elute faster than hydrophobic molecules. Long alkyl chain or aromatic compounds will elute late or require an organic solvent (CH3CN or CH3OH, max. of 15v/v%) to prevent absorption onto the stationary phase. Advantages: The CRS series columns separate over 20 D,L--Amino acids by only single column. The columns separate not only -Amino acids but also -Hydroxy carboxylic acids and derivative amino acids such as Acetylated amino acids. The columns provide excellent resolution operated at room temperature. The columns show high durability.
Separation mechanisms and characteristics
Application of CRS10W DL-Isoleucine
Separation of amino acids mixture
Separation of DL-tryptophan
Durability test
Cat. #
021901 022001
Description
CRS10W (DLAA) CRS15W (LDAA)
Particle Size
3m 3m 100 100
Pore Size
ID x L
4.6 x 50mm 4.6 x 50mm
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HPLC / GPC - Jordi

Jordi Gel Permeation Chromatography of Oligomers and Polymers
Jordi FLP manufactures a complete line of polymer-based high-pressure liquid chromatography columns specializing in size exclusion chromatography. All Jordi columns are made from 100% divinylbenzene providing maximum column life, improved resolution and more linear calibration curves due to the high pore volume of the Jordi columns. Additional benefits of choosing Jordi columns: Rugged - Jordis divinylbenzene packing can be run at high temperatures in nearly all solvents. Change solvents without damaging the Jordi column. Most Jordi packings can be used at temperatures of up to 150C and pressures up to 30,000 psi. pH Resistant - Jordi columns are usable over the entire pH range allowing the Jordi columns to support mobile phase & pH values 0 - 14. Most competitive silica-based columns typically operate in the pH 2 - 7 range. Highly efficient - High plate counts for sharp, symmetrical peaks. Powerful separation tool - High pore-volume for greater resolution with fewer columns, as well as porosities to cover the complete molecular weight range. Jordi columns use a larger standard column dimension of 10mm (industry norm columns are 7.8mm) at low cost. Versatile - Largest selection of surface chemistries available, providing the greatest opportunity for a successful separation. Jordi offers a complete line of GPC columns in analytical, semi-prep, and prep sizes. For more information about the Jordi line of products and their separation capabilities, please visit our website or contact your Product Specialist.
Cat. #
15025 90015 15065 16506 16508 18501 18601

Description
Jordi Gel DVB Column (MW range = 100 - 10,000,000 g/mol) Jordi Gel DVB Flourinated Column (MW range = 100 - 10,000,000 g/mol) Jordi Gel DVB Glucose Column (MW range = 100 - 10,000,000 g/mol) Aqueous GPC Jordi Gel DVB Reverse Phase Column Jordi Gel DVB Reverse Phase Column Jordi Gel DVB Reverse Phase C18 Column
Pore Size
Mixed-Bed Mixed-Bed Mixed-Bed 300 1000 500 500
Part. Size
5m 5m 5m 5m 5m 5m 5m
ID x L
10 x 250mm 10 x 250mm 10 x 250mm 4.6 x 250mm 4.6 x 250mm 4.6 x 250mm 4.6 x 250mm
Jordi Gel NP DVB Glucose Column (MW range = 100 - 10,000,000 g/mol)
In addition to Jordis complete line of polymer-based gel permeation chromatography (GPC) columns, we offer a full line of SPE Cartridges (see page 26) and Well Plates as well as Bulk Packing Media (see page 17). All Jordi GPC products are made using their high quality 100% divinylbenzene packing materials which have been in continuous production for over 25 years. Jordi uses only the highest quality resins in all of their GPC products. These products are thoroughly tested to ensure consistency from batch to batch including verification of particle shape, size and purity as well as chromatographic performance.
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HPLC / GPC - Jordi xStream Column
Use a single Jordi xStream H2O in 100% aqueous, 100% organic or any mobile phase by simply changing solvents.
Jordi xStream H2O columns represent a breakthrough in GPC column technology. This revolutionary column allows for the broadest range of separations currently obtainable with any GPC column. xStream columns were designed for aqueous GPC separations including the analysis of dextrans and polysaccharides. It performs equally well for the analysis of organic soluble polymers including the analysis of polystyrene or other common polymers in THF or chloroform. It is also an excellent choice for the analysis of nylons and poly(ethyleneterepthalate) in hexafluoroisopropanol (HFIP). A single xStream column can be used for aqueous or organic GPC analysis by simply changing solvents. xStream H2O GPC columns are an excellent column for aqueous GPC analyses. This includes the analysis of a wide range of aqueous soluble polymers including polysaccharides such as dextrans and starches. Other polymers which can be analyzed include butyl methacrylate / isobutyl methacrylate copolymer, phenoxy resin, poly(n-butyl methacrylate), polycaprolactone and poly(isobutyl methacrylate). In addition, the presence of salts in the mobile phase can be used for the analysis of cationic polymers such as quaternary amine polymers. The broad solvent compatibility of xStream H2O GPC columns allows for their application in all common organic solvents. This includes analyses in polar organic solvents such as DMSO, DMF and DMAC as well as more hydrophobic solvents such as THF and chloroform. Examples of polymer systems which have been analyzed include poly(methyl methacrylate), poly(vinyl chloride) and copolymers of acrylonitrile, styrene, butadiene, butyl methacrylate. Advantages of Jordi xStream H2O GPC columns: Separation - Broadest applicability of any currently available GPC column Mw Range - up to 700K molecular weight in H2O, up to 10 million molecular weight in organic solvents Solvent Compatibility - water and all common organic solvents Wide Range of Porosities - 1000 to 105, Mixed Bed and SB Rugged - long column life - 3 month warranty standard Quality - subjected to rigorous quality checks
Poly(methyl methacrylate) in HFIP
Polysaccharides in H2O
Brown - Methyl Vinyl Ether-Malecacid Copolymer - Pure H2O Blue - Polysufone - DMAC Red - Sodium Alginate - H2O/MEOH/0.01M EDTA Green - Polyvinyl Chloride - THF
Color
Jordi xStream
Cat. #
33022 33024 33025
Description
Jordi xStream H2O Column Jordi xStream H2O Column Jordi xStream H2O Column 1000
Pore Size
100000 Mixed Bed
Particle Size
5m 5m 5m
ID x L
10 x 250mm 10 x 250mm 10 x 250mm
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77
HPLC Column Packing System
Pack your own HPLC columns and Save Money

SSI Lab Alliance - Pack in a Box The Portable HPLC Column Packing System
Pack-in-a-Box includes the following:
1. Model CP Constant Pressure Pump, non-flush, 0.1-24 mL/min., 10,000 psig, with transducer and RS-232 control, SS, 110/220V 2. Two empty column assemblies, 4.6 x 150 mm (column blanks, column plugs, end fittings, 2 m frits) 3. Column Packer assembly (20 mL reservoir, nuts, caps, ferrules, column adapter 5/16 4.6 mm, gasket, tubing) 4. Packer Bracket Assembly (bracket, rods, bolts, knobs, nuts) 5. C18 Spherical Silica Gel, 10g 6. Tubing, 1/16 OD x 0.030 ID, Stainless steel 7. Quick-Set pump control software 8. Instructions
Complete Pump Control

Fast or Pressure limited ramps Ramp speed adjustments Packing pressure Packing Flow Upper and lower pressure limits
Cat. #
150050 011191 011291 011289 150026 150034 150027 Pack-in-a-Box kit
Product
Column packer reservoir, 20 mL, 5-3/4 in. Column packer reservoir, 60 mL, 15 in. Column packer reservoir, 500 mL Column assembly, 2.1 x 150mm x 2 m Column assembly, 4.6 x 150mm x 2 m Column assembly, 4.6 x 250mm x 2 m
Affordable and perfect for universities and research labs.
NOTE: Pack-in-a-Box has many optional parts to fit a variety of HPLC Columns For more selections and sizes, visit our website or contact your personal Product Specialist 78 Sorbent Technologies, Inc. Toll Free: 866.SORBTEC Fax: 770.936.0326
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Prep Column Packing System

Sorbent Technologies now offers the Prep Column Packing system to enable you to custom pack your own Prep HPLC Columns. This system includes the HF300CP High Performance dual piston pump specifically designed for packing columns and Packing Station to pack 10mm, 21mm, 30mm, & 50 mm ID x 250mm Length columns.
HF300CP High Flow Dual Piston Pump Constant Pressure

Dual-Head Design for very Accurate Flow Rates and Low Pulsation Servo Motor, Belt Drive Mechanism, with Reliable Eccentric Cams Ceramic Pistons and Ruby/Sapphire Check Valves (3/16 ball; single capsule) Prime-Purge Valve, Pulse Dampener (optional), and Outlet Filter Pressure Transducer (in T or Pulse Dampener) with Upper/Lower Pressure Limits Constant-Pressure Control Mode (LC Column Packing) Automatic Pressure Compensation and User Flow Calibration Features Universal Voltage Input: 90-260 VAC / 50-60 (300 mL/min. pumps require 220 VAC for 10,000 psi) Constant Flow Performance with varying Back Pressure Stainless Steel Fluid Path & Industrial Stainless Steel Enclosure Motor Stall Detector Interactive LCD Keypad
HF300CP Specifications
Flow Rates..0.1 - 300.0 mL/min. Pressure..CP 10,000 psi, CF 8,500 psi Flow Accuracy..2% of set point Flow Precision..0.5% RSD Pulsation.. 5.0% @ 150 mL/min., 3,000 psi Dimensions..9 W x 14 H x 22 D (22.8 x 35.6 x 55.9 cm) Weight..50 lbs. (22.7 kg)
Packing Station
Enables you to pack 10, 21.2, 30, & 50 mm ID columns x 250mm length Different Reservoirs & Column Tubes available for the specific size columns you want to pack Stainless Steel
Cat. #
P25SNXP1 3140-21 2001-19212-025 6004IG-P 7353D-20H 2001-10100-025EP 6007IG-P 7352-20H 3132 3132 01 2050-56500-005 3113-SR 3140-50-30 286SH3216 2001-24300-010 2050-56500-025 7751D-20H 6751I-P Misc.
Discription
CP 250 Pump, 0.1250.0 mL/min, constant pressure PACKING SYSTEM, FOR A 21.2mm COLUMN COLUMN TUBE, MODULAR, 21.2mm X 25cm COLUMN END FITTING, G.T.P., 1/16 INCH PORT IT/PARKER, 21.2MM (requires 2) ASSEMBLY, FRIT CAP, HIFLO DISPERSION, EXTERNAL, 21.2mm, 2m (requires 2) COLUMN TUBE, MODULAR, 10mm X 25cm COLUMN END FITTING, G.T.P., 10.0mm, IT/PARKER (requires 2) ASSEMBLY, FRIT CAP, OPEN FACE, 10.0mm, 2um (requires 2) PACKING COUPLER 10MM SEAL, 10mm PACKING COUPLER (requires 2) COLUMN TUBE, 50mm X 5cm SEAL RING, 50mm COLUMN SYSTEM (requires 3) PACKING ADAPTER ASSEMBLY, 50mm TUBE TO 30mm TUBE SCREW, 5/16-24 X 1 SOCKET HEAD CAP, HEAT RESISTANT (requires 12 MODULAR COLUMN TUBE, 30mm X 10cm COLUMN TUBE, ISO-PREP, 50mm X 25cm FILTER ASSEMBLY, 50mm X 2um (requires 2) END CAP, 50mm COLUMN SYSTEM, IT/PARKER (requires 2) 5 section of Capillary TUBE 1/16IN X .020 X 5 FT
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79
HPLC Systems
Extreme HPLC System
Why Make Laevitas Your Primary UHPLC system?
Laevitas is designed to recognize the development of orthogonal separation techniques, especially the liquid phase separations of complex mixtures to obtain comprehensive results that are increasingly demanded by regulatory agencies. Laevitas UHPLC system features a modular designed that enables numerous system configurations. The system consists of three major compartments: an auto-sampling system that ensures carryover of less than 0.01% with optional sample cooling, a temperature protected oven and detection module, and a high-precision pumping system that delivers consistent, reliable solvent flow. The modules are automated and designed for heavy sample load on a daily basis. Laevitas is designed to offer technological solutions to the rising demands for high resolution and maximum peak capacity of low level complex sample identification and quantifications. Laevitas green technology is its micro flow rate which uses far less solvent than the traditional HPLC. The micro flow rate minimizes the consumption of organic solvents, avoiding the generation of large amounts of toxic waste and consequently inflicting no harm on the environment. The industrial design requires less bench area, which adds more value in space arrangement in the modern lab. Among chemists and scientists, the comments about Laevitas is It is not just a UHPLC, but a piece of art in our lab.
Condition
Column: Inertsil ODS-4 (2m, 50 x 2.1 mm I.D.) Eluent: CH3N/H2O = 65/35 Flow Rate: 0.6 mL/min Injection Vol: 1 L Col. Temp.: 40 C Detection: UV 254 nm
Laevitas 800
Sample
1. Acetophenone 2. Benzene 3. Toluene 4. Naphthalen
Others
80
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Laevitas 800 Features
Laevitas 800 brings users an uncompromising performance in efficiency, precision, separtion & cost-saving. The Minimum Dead Volume: <170m from point of gradient mixing to analytical column The Minimum Dead Volume: <3.4 l Least Carry-Over: <0.1% (standard Wash); <0.0005 (Custom Wash) Unmatchable Reliability: < 0.1%RSD (Flow Precision)
Low Carry Over Autosampler
Laevitas 800s unique injector washing system enhances the elimination of carryovers, even in high sensitivity analysis.
System: Laevitas 800 + 4000 QTRAP Column: Inertsil ODS-3 2m 2.1 x 50mm Eluent: (A) 0.1% Formic acid aq (B) 0.1% Formic acid in aceton A/B=70/30, v/v Flow Rate: 0.4mL/min Col. Temp.: 40 C Detection: MS/MS (ESI, Pos, MRM)
Precise gradient composition

Laevitas 800 pump delivers precise competition which enhances separation resolution. The unique design of the instrument also ensures low dead volume, which results in less extra-column band broadening. This provides users with greater flexibility in choosing shorter and narrower ID columns, which provide sharper peaks and shorter run times.
Column: Inertsil ODS-3 2m 2.1mmI.D x 50mm Flow Rate: 0.6mL/min Eluent: (A) 0.1% TFA water solution (B) 0.1% Acetonitrile including TFA A/B=85/15 - 1 min - 65/35 - 0.4 min - 65/35 Col. Temp.: 40 C Detection: UV214 nm
Benefits of temperature control

When performing very high speed analysis, it is possible that the retention time can become irreproducible due to fluctuations in mobile phase temperature. Laevitas 800 offers complete hermostatic stability by building the gradient mixer inside the column oven; the result is excellent retention time reproducibility.
Column: Inertsil ODS-3 2m 2.1mmI.D x 50mm Flow Rate: 0.2mL/min Eluent: (A) 0.05% TFA in CH3OH (B) H2O A/B=25/75 Detection: UV214 nm Ultraviolet
Detector (ECD) Flow Cell
Electrochemical Detector (ECD) Flow Cell

An analysis with Electrochemical Detector :

Using Electrochemical Detector and Ultraviolet Detector to analyze complex mixture of acid/basic sample. System: Laevitas 800 Column: Inertsil ODS-3 (Column size refer in chromatogram) Flow Rate: 0.2mL/min, 0.6mL/min (Refer in chromatogram) Eluent: A) Methanol B) 16mM Citric Acid, 18mM Kh2PO4aq, 240 mg/L SOS, 50.8 mg/L EDTA .2Na A/B = 15/85 (v/v)v/v (gradient mixer) Col. Temp.: 35C Detection: ECD 1100mV response 1.0s Injection Vol.: 5uL (1ppm each) Sample 1. Norepinephrine (NE) 2. 3, 4-Dihydroxyphenylacetic acid (DOPAC) 3. 3, 4- Dihydroxybenzylamine (DHBA) 4. 5- Hydroxyindolacetic acid (5 - HIAA) 5. 3- Hydroxytyramine hydrochloride )DA_ 6. Homovanillic acid (HVA) 7. Serotonin hydrochloride (5-HT)
High separation efficiency, Minimized dead volume

Analysis with Laevitas 800 offer the sharper peaks with increased peak height and leads to increased sensitivity. Laevitas 800 system can be used with both a general purpose detector, such as UV-visable, etc., also LC/MS detectors
Melarmine and Cyanuricacida analysis by using LC/MS

System: Laevitas 800 4000QTRAP Column: Inertsil HILIC (5 m, 150x 3.0mm I.D.) A) CHCN B) 10mM Ammonium acetate A / B =85 / 15 -0.5mm -85 / 15-6.5mm -50 / 50 (Equilibrate 8mm), v/v Flow Rate: 0.5 mL/min Col. Temp.: 40C Detection: MRM (SRM) Injection Vol.: 5L Sample 1. Cyanuricacid 50 ug/L 2. Melamine 50 ug/L Compound Qi Q3 Melamine 126.9 85 126.9 68 Cyanuric acid 127.9 42 127.9 84.9
82
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Antihistamines Analysis by using IC/MS

System: Laevitas 800 4000QTRAP Column: Inertsil ODS-4(3m, 150x2.1 mm I.D.) Eluent: A) CHOH B)2mM Ammonium acetate A/B = 40/60 - 10 mm- 95/5-2 mm -95/5 Flow Rate: 0.2 mL/min Col. Temp.: 40C Detection: MS/MS (4000QTRAP:ESI, Posi, MRM) Injection Vol.: 10L Sample: Q1 1. Chlorpheniramine (0.1 mg/L) 229.9 2. Cinnarizin (0.1 mg/L) 369.0 3. Clemastine (0.1 mg/L) 344 0 4. Difenidol (0.1 mg/L) 310.0 5. Diphenhydramine. (0.1 mg/L) 255.8 6. Diphenylpyraline (0.1 mg/L) 282.0 7. Hydroxyzine (0.1 mg/L) 375.0 8. Promethazine (0.1 mg/L) 284.0 9. Triprolidine (0.1 mg/L) 278.9
Specification
Auto-sampler Number of Sample Plates:
Column Oven / UV-VIS Module

1.5 ml Vial (360 vials) 4 ml Vial (256 Vials) 96 arid 384 Well Plate (6 Plates)
Injection Volumes: 0.1 L 50 L, with 0.1 L increments Injection Methods: Full loop, partial loop fill and no waste pickup fill Injection Precision: <0.3% RSD Carry Over: <0.01% with needle wash Wash Solvents: Two solvents Sample Cooling: 4C to 40C, at least 16C below ambient Injection Cycle Time: <30 sec
Column Temperature Control: Ambient -10C to 60C (1C increments) Column Size: OD 63 to 8.0, Length (max.) 250 mm. Lamp source: Deuterium lamp Wavelength Modes: Single and dual wavelength modes, user- selectable Wavelength range: 190 to 700 nm Wavelength Accuracy: 1 nm Wavelength Precision: 0.1 nm Noise: < 0.6 x 10-AU at 254 nm Drift: 2 x 10- AU/Hr at 254 nm Data Acquisitions: up to 200 Hz Flow Cell: Volume 1 L (pathlength 5 mm)
Pump & Solvent Control
Low Carry-Over Auto Sampler
Number of Solvents: One or Two Operating flow Rate Range: 1 L/min to 2000 L/min Gradient Type: High-pressure mixing Operating Pressure Max, 12,000 psi Flow Rate Precision: < 0.1% RSD Flow Accuracy: < 1.0% Gradient Range: 0 ~ 100% (1% increments) Delay Dead Volume: <170 L from point of gradient mixing to analytical column Solvent Degassing: 2 channels Plunger Seal Wash: Integral, active
Contact a product specialist for more information about additional HPLC systems: Envision Nano 2000, Impressio Mirco 2000, and Atima 300/400. The Envision Nano 2000 has a sub-micro pumping system designed to optimize nano-flow separations and significantly reduces solvent consumptions The Atima 300/400 Standard HPLC system is ideal for everyday separation.
GC Columns
Macherey-Nagel Optima High Performance GC & GC/MS Columns
Ultra-High Performance Increased Selectivity High Thermal Stability Maximum Operating Temperatures Lower Bleed Levels Extremely Inert
Macherey-Nagels Optima series columns for Capillary GC and GC-MS provide ultra-high performance and selectivity. Optima 5-MS Accent & LRB are the next generation. A special treatment after immobilization of the phase results in lower column bleeding, higher temperature range and large sample volume injection. Optima -3 & -6 are proprietary phases with a wide range of polarities for autoselectivity that is excellent for organophosphorus pesticides (EPA 8140/8141). All columns are available in a wide selection of stationary phases, ID, length, and film thickness. Each column is tested to insure reproducibility. Application areas: environmental analysis, trace analysis, EPA methods, pesticides, PCBs, food and drug analysis, and special applications i.e. enantiomer analyses.
Comparison of separation properties of selected OPTIMA

1 2 3 5 1 2 3 5 6 74 8 9 10 6 74 8 9 10
OPTIMA
240
max. temp. 260/280 C Cat. No. 726096.30
OPTIMA
225
max. temp. 260/280 C Cat. No. 726084.30
1 2 3
4 6
8 9
OPTIMA
10
210
max. temp. 260/280 C Cat. No. 726880.30
1 3
2 5
9 10
OPTIMA
1701
max. temp. 320/340 C Cat. No. 726320.30
2 5
6 7
8 9 10
OPTIMA
17
3 1 4 25
8 9 10
OPTIMA
max. temp. 340/360 C Cat. No. 726316.30 2 5 6 7 8 9 10
1 3
OPTIMA
max. temp. 340/360 C Cat. No. 726304.30
10
20
min
30
All columns: Sample: Injection: Carrier gas: Temperature: Detector:
0.5 m lm, 30 m x 0.32 mm ID MN-OPTIMA test mixture (Cat. No. 722316) 1.0 l, split 1:50 80 kPa N 2 80 C T max (isothermal), 8 C/min 6 FID, 260 300 C, 2
Peaks: 1. Undecane 2. Dodecane 3. Octanol 4. Dimethylaniline 5. Decylamine
6. Methyl decanoate 7. Methyl undecanoate 8. Henicosane 9. Docosane 10. Tricosane
84
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increasing polarity
max. temp. 320/340 C Cat. No. 726744.30
Stationary Phase Cross-Reference for Other Manufacturers
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85
OPTIMA 1
100 % dimethylpolysiloxane
Nonpolar phase Similar phases: PERMABOND SE-30, OV-1, DB-1, SE-30, HP-1, SPB-1, CP-Sil 5 CB, Rtx-1, 007-1, BP1, MDN-1, AT-1, ZB-1, OV-101 For columns with 0.1 0.32 mm ID and films < 3 m the max. temperature for isothermal operation is 340 C and the max. temperature for short isotherms in a temperature program is 360 C For 0.53 mm ID columns with films < 3 m the max. temperatures are 320 and 340 C, resp. For thick film columns with films 3 m the max. temperatures are 300 and 320 C, resp. Separation of components according to boiling points Thick film columns 3 m film are especially recommended for solvent analysis USP G1 / G2 / G38
OPTIMA 1 MS
Selectivity identical to OPTIMA 1 Similar phases: Ultra-1, DB-1MS, HP-1MS, Rtx- 1MS, Equity-1, AT-1MS, VF-1MS, CP-Sil 5 CB MS Max. temperature for isothermal operation 340 C, max. temperature for short isotherms in a temperature program 360 C Phase with low bleeding Ideal for GC/MS and ECD applications and general analyses at trace level USP G1 / G2 / G38
86
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OPTIMA 1 MS Accent
Selectivity identical to OPTIMA 1 Similar phases: Ultra-1, DB-1 MS,HP-1 MS, Rtx-1 MS, Equity-1, AT-1 MS, VF-1 MS, CP-Sil 5 CB MS
Increased sensitivity due to an unmatched low background level Max. temperature for isothermal operation 340 C Max. temperature for short isotherms in a temperature program 360 C Lowest column bleed, non-polar phase, ideal for ion trap and quadrupol MS detectors Perfect inertness for basic compounds Solvent rinsing for removal of impurities applicable Application areas: all-round phase for environmental analyses, trace analyses, EPA methods, pesticides, PCB, food and drug analyses USP G1 / G2 / G38
OPTIMA 1 MS Accent, 0.50 m lm, 30 m x 0.32 mm ID , Cat. No. 725807.30 Sample: 0.2 g/ml in hexane, 8140/8141 OP pesticides calibr ation mix A and 8141 OP pesticides calibration mix B; IS tr iphenyl phosphate and tributyl phosphate Injection: splitless (hold 1 min) Inj. temperature: 250 C Carrier gas: He, 1 ml/min, constant pressure Temperature: 100 C 180 C, 10 C/min (2 min) 300 C, 18 C/min (3 min) Detector : FPD (Flame Photometric Detector), 280 C Peaks: 1. Dichlorvos, 2. Hexamethylphosphoramide, 3. Mevinphos, 4. Trichlorfon, 5. TEPP, 6. Thionazin, 7. Demeton-0, 8. Ethoprop 9. Tributyl phosphate (IS), 10. Dicrotophos, 11. Monocrotophos, 12. Naled, 13. Sulfotepp, 14. Phorate, 15. Dimethoate, 16. Demeton-S, 17. Dioxathion, 18. Terbufos, 19. Fonophos, 20. Phosphamidon isomer, 21. Diazinon, 22. Disulfoton, 23. Phosphamidon, 24. Dichlorofenthion, 25. Parathion-methyl, 26. Chlorpyrifos methyl, 27. Ronnel, 28. Fenitrothion, 29. Malathion, 30. Fenthion, 31. Aspon, 32. Parathion-ethyl, 33. Chlorpyrifos, 34. Trichloronate, 35. Chlorfenvinphos, 36. Merphos, 37. Crotoxyphos, 38. Stirofos, 39. Tokuthion, 40. Merphos oxidation product, 41. Fensulfothion, 42. Famphur, 43. Ethion, 44. Bolstar, 45. Carbophenothion, 46. Triphenyl phosphate (IS), 47. Phosmet, 48. EPN, 49. Azinphos-methyl, 50. Leptophos, 51. Trio-cresyl phosphate, 52. Azinphos-ethyl, 53. Coumaphos Column:
EPA 8140 / 8141 / 8141 A Organophosphorus pesticides

2 31 32 33 13 24 25 43 44 42 51
6 9 10 11 12 8 3 1 4 7 16 17 5 15 14
18 19 45 48 46 47 41 34 38 35 39 40 49 50 52 53
27 30 36 26 28 37 29 21 22 23
20
MN Appl. No. 213030
12
16 min
Cat. #
726050.30 706302.10 726302.30 726304.30 726304.60 726205.30 726202.60 725801.25 725805.30 725807.30 Optima 1 Optima 1 Optima 1 Optima 1 Optima 1 Optima 1 MS Optima 1 MS
Description
ID mm
0.25mm 0.32mm 0.32mm 0.32mm 0.32mm 0.25mm 0.32mm 0.2mm 0.25mm 0.5mm
Length
30m 10m 30m
Film Thickness
0.25m 0.25m 0.25m 0.50m 0.50m 0.25m 0.25m 0.20m 0.25m 0.5m
max temp 340/360 C 340/360 C 340/360 C 340/360 C 340/360 C 340/360 C 340/360 C 340/360 C 340/360 C 340/360 C 87
30m 60m
30m 60m 25m 30m 30m
Optima 1 MS Accent Optima 1 MS Accent Optima 1 MS Accent
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OPTIMA 5
5 % phenyl 95 % methylpolysiloxane
Nonpolar phase Similar phases: PERMABOND SE-52, SE-54, SE-52, DB-5, HP-5, SPB-5, CP-Sil 8, Rtx-5, 007-5, BP5, MDN-5, AT-5, ZB-5 For columns with 0.1 0.32 mm D and films < 3 m max. temperature for isothermal operation is 340 C and max. temperature for short isotherms in a temperature program is 360 C For 0.53 mm ID columns with films < 3 m max. temperatures are 320 and 340 C, resp. For thick film columns with films 3 m the max. temperatures are 300 and 320 C, resp. Standard phase with large range of application USP G27 / G36
OPTIMA 5 MS
5 % diphenyl 95 % dimethylpolysiloxane
Selectivity identical to OPTIMA 5 Similar phases see OPTIMA 5 MS Accent Max. temperature for isothermal operation 340 C Max. temperature for short isotherms in a temperature program 360 C Phase with low bleeding- Ideal for GC/MS and ECD applications and general analyses at trace level perfect inertness for basic compounds USP G27 / G36
Analysis of various phenols

Column: OPTIMA 5 MS, 30 m x 0.25 mm ID, 0.25 m lm, Cat. No. 726220.30, max. temperature 340/360 C 5 ppm of each compound except N -i-propylaniline (9.4 ppm) SPME 40 C (2 min) 240 C, 6 C/min 320 C, 20 C/min MSD 12 8 14
Sample: Method: Temperature: Detector : Peaks: 1. Toluene-D 8 2. Phenol 3. 2-Methylphenol ( o-Cresol) 4. Nitrobenzene-D 5 5. N -i-Propylaniline 6. 2,4-Dichlorophenol 7. 4-Chlorophenol 8. 4-Bromo-2-chlorophenol 9. 3-Bromophenol 10. 4-Chloro-3-methylphenol 11. 2,4-Dibromophenol 12. 2-Hydroxybiphenyl 13. 2-Cyclohexylphenol 14. Hexauorobisphenol A
10 5 9
7 1
34
Courtesy of Riedel-de-Han, Seelze, Germany 2 6

MN Appl. No. 210110
13 11
20 30 min
10
88
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OPTIMA 5-MS Accent
silarylene phase
Chemically bonded, cross-linked silarylene phase with polarity similar to a 5 % diphenyl - 95 % dimethylpolysiloxane phase Similar phases: DB-5 MS, HP-5 MS, Ultra-2, Equity-5, CP-Sil 8 CB low bleed/MS, Rtx-5SIL-MS, Rtx-5 MS, 007-5 MS, BPX5, MDN-5S, AT-5 MS, VF-5 MS Increased sensitivity due to an unmatched low background level Max. temperature for isothermal operation 340 C Max. temperature for short isotherms in a temperature program 360 C For columns with films > 0.5 m max. temperatures are 320 and 340 C, resp. Lowest column bleed, nonpolar phase, ideal for ion trap and quadrupol MS detectors. Solvent rinsing for removal of impurities applicable Application areas: all-round phase for environmental analyses, trace analyses, EPA methods, pesticides, PCB, food and drug analyses USP G27 / G36
Baseline Separation of PAHs Column: OPTIMA 5 MS Accent, 30 m x 0.25 mm ID, 0.25 m film Phase: OPTIMA 5 MS Accent Cat #: 725820.30 Sample pretreatment: Sample: 1l of 20 ng/l, PAH mix Conditions: Injection volume: 1 ul splitless (for 1 min) Injection temperature: 300 C Carrier gas: Hydrogen, 40 cm/sec Temperature: 40 C for 1 min --> 200 C, 20 C/min --> 310 C, 4 C/min for 5 min Detection subsequent analysis: FID, 310 C
Legend: 1. naphthalene 2. acenaphthylene 3. acenapthene
4. fluorene 5. phenanthrene 6. anthracene
7. fluoranthene 8. pyrene 9. benzo(a)anthracene
10. chrysene 11. benzo(b)fluoranthene 12. benzo(k)fluoranthene
13. benzo(a)pyrene 14. indeno(1,2,3-cd)pyrene 15. dibenzo(a,h)anthracene 16. benzo(ghi)perylene
OPTIMA 5-MS Accent Background Noise at 340

107.8 90.2
standard 5 MS phase
FID signal in mV
107.6 90
107.4
89.8
107.2
89.6
OPTIMA
10
5 MS Accent
20 30 s
10
20
30 s
Cat. #
726056.30 726628.60 726220.30 725811.30 726354.30 Optima 5 Optima 5 Optima 5 MS
Description
ID mm
0.25mm 0.32mm 0.25mm 0.32mm 0.25mm
Length
30m 60m 30m 30m 30m
Film Thickness
0.25m 0.35m 0.25m 0.25m 0.5m
max temp 340/360 C 340/360 C 340/360 C 340/360 C 340/360 C 89
Optima 5 MS Accent Optima 5 Amine
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OPTIMA 5 HT NEW!
Chemically bonded, cross-linked silarylene phase, polarity index according to 5 % phenyl / 95 % methylpolysiloxane Similar phases: DB-5HT, VF-5HT, HT-5,XTI-5HT, ZB-5HT Max. temperature for isothermal operation 380 C Max. temperature for short isotherms in a temperature programme 400 C Low bleed column, nonpolar phase, ideal for MS detectors, can be rinsed with solvents USP G27 / G36
Column stability for the example of a separation of 10 components over 180 hours at 380 C isothermal
Column: OPTIMA 5 HT, 15 m x 0.25 mm ID, lm 0.1 m Peaks: 1. Undecane 2. Dodecane 3. Octanol 4. Dimethylaniline 5. Decylamine 6. Methyl decanoate 7. Methyl undecanoate 8. Henicosane 9. Docosane 10. Tricosane
Rapid Determination of Diesel Sample With Calibration via a Steep Heating Rate Substances: diesel ingredients; alkanes (C10 - C24) Column: OPTIMA 5 HT, 15 m x 0.25 mm ID, 0.10 m film Phase: OPTIMA 5 HT Cat #: 726102.15 Sample: Diesel, 0.5 mg/ml in CH2Cl2 Calibration mix: Alkanes C10 - C24 in CH2Cl2 Conditions: Injection volume: 1.0 l, splitless (for 0.2 min), then split 100 ml/min Injection temperature: 230 C Carrier gas: Helium, 0.8 bar Temperature: 40 C for 1 min --> 360 C, 50 C/min for 20 min Detection: FID, 280 C Legend: Sample: Diesel, 0.5 mg/ml in CH2Cl2 Calibration mix: Alkanes C10 - C24 in CH2Cl2
Cat. #
726102.15 726106.30 726108.15 726108.30 Optima 5HT Optima 5HT Optima 5HT Optima 5HT
Description
ID mm
0.25mm 0.25mm 0.32mm 0.32mm
Length
15m 30m 15m 30m
Film Thickness
0.10m 0.25m 0.25m 0.25m
max temp 340/360 C 340/360 C 340/360 C 340/360 C
90
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OPTIMA XLB NEW!
silarylene phase
Chemically bonded, cross-linked silarylene phase, optimized silarylene content for lowest column bleed Similar phases: DB-XLB, Rtx-XLB, MDN-12, VF-XMS Max. temperature for isothermal operation 340 C, Max. temperature for short isotherms in a temperature program 360 C Lowest column bleed, nonpolar phase, ideal for ion trap and quadrupol MS detectors Perfect inertness for basic compounds Solvent rinsing for removal of impurities applicable Application areas: ultra low bleed phase, highly selective for environmental and trace analyses, pesticides recommended phase for PCB separations
Rapid separation of PCB and PAH
Column: Injection volume: Injection: Purge ow: Temperature: Detection: OPTIMA XLB, 0.25 m lm, 30 m x 0.25 mm ID, Cat. No. 725850.30 1 l, standard 0.005 ng/l 250 C, pulsed, splitless, pulse 1.38 bar in 1 min 60 ml/min He 40 C (2 min) 240 C (2 min), 30 C/min 340 C (5 min), 10 C/min MS source 230 C, interface 280 C, quadrupol 150 C 4 8: PCB-31 9: PCB-28 5 3 1 2 6 7 82 % separation in less than 10 min! 25 26 benzo[b]uoranthene / benzo[b]uoranthene 75 % separation in 17 min! 15 21 8/9 10
6 8 10
11 13
22 25 26 27
29 28
30
12 14 16 17 18 19 20
12 14
23
24
16 18 20 min
Courtesy of Centre dAnalyses de Recherche, Lab. dHydrologie, F-65400 Illkirch, France
MN Appl. No. 212920
Peaks: 1. Naphthalene 2. 2-Methylnaphthalene 3. Acenaphthylene 4. Acenaphthene 5. Fluorene 6. Phenanthrene 7. Anthracene 8. PCB-31 9. PCB-28 10. PCB-52 11. Fluoranthene 12. PCB-101 13. Pyrene 14. PCB-77 15. 2-Methyluoranthene 16. PCB-118 17. PCB-153 18. PCB-138 19. PCB-126 20. PCB-180 21. Benz[a]anthracene 22. Chrysene 23. PCB-169 24. PCB-194 25. Benzo[b]uoranthene 26. Benzo[k]uoranthene 27. Benzo[a]pyrene 28. Dibenz[ah]anthracene 29. Indeno[1,2,3-cd)pyrene 30. Benzo[ghi]perylene
Determination of PCBs and PAHs from Slag Legend: 1. naphthalene 2. 2-methylnaphthalene 3. acenaphthene 4. fluorene 5. phenanthrene 6. anthracene 7. PCB 31 8. PCB 28 9. PCB 52 10. fluoranthene 11. PCB 101 12. pyrene 13. PCB 118 14. PCB 153 15. PCB 138 16. PCB 180 Column: OPTIMA XLB, 30 m, 0.25 mm, 0.25 m Phase: OPTIMA XLB Cat #: 725850.30 Sample pretreatment: Sample from slag Conditions: Injection volume: 1 l Injection temperature: 250 C Injection pulse: 1.38 bar in 1 min; splittless Carrier gas: Helium, with purge flow of 60 ml/min Temperature program: 40 C for 2 min, 40 C -> 240 C with 30 C/min, 240 C for 2 min, 240 C -> 340 C with 10 C/min, 340 C for 5 min Detection: GC-MS: Interface temperature: 280 C Ion source temperature: 230 C Quadrupol temperature: 150 C Author(-s): Centre dAnalyses de Recherche Source: Lab. DHydrologie, Illkirch (France), 2007
Cat. #
725850.30 725850.60 Optima XLB Optima XLB
Description
ID mm
0.25mm 0.25mm
Length
30m 60m
Film Thickness
0.25m 0.25m
max temp 340/360 C 340/360 C 91
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OPTIMA -3 - polysiloxane phase with autoselectivity

Medium polar without CN groups Analytes determine the polarity of the phase Unique, no similar phase Ideal for MSD and PND detectors Max. temperature for isothermal operation 340 C Max. temperature for short isotherms in a temperature program 360 C USP G49
Autoselectivity resulting in a wide range of polarities from approximately the non-polar OPTIMA 5 to the midpolar OPTIMA 1701
Analysis of isomeric phenols
Column: OPTIMA -3, 0.25 m lm, 60 m x 0.25 mm ID, max. temperature 340/360 C, Cat. No. 726420.60 Injection: 1.0 l, split 1:80 8 5 Carrier gas: He, 1.3 bar 6 2 9 Temperature: 60 C (3 min) 320 C, 6 C/min 3 Detector : MSD HP 5971 13 Peaks: 4 12 1. Phenol 15 2. 2-Chlorophenol 10 14 3. 2-Methylphenol 1 11 4. 4-Methylphenol 5. 3-Methylphenol 6. 2,4-Dimethylphenol 18 7. 2-Nitrophenol 7 8. 2,4-Dichlorophenol 17 9. 2,6-Dichlorophenol 10. 4-Chloro-3-methylphenol 16 20 11. 2,3,5-Trichlorophenol 12. 2,4,6-Trichlorophenol 21 13. 2,4,5-Trichlorophenol 14. 2,3,4-Trichlorophenol 19 15. 2,3,6-Trichlorophenol 16. 2,3,5,6-Tetrachlorophenol 17. 2,3,4,5-Tetrachlorophenol 18. 2,3,4,6-Tetrachlorophenol 19. 2,4-Dinitrophenol 20. 3,4,5-Trichlorophenol 21. 2-Methyl-4,6-dinitrophenol 10 20 22. 2-Isopropyl-4,6-dinitrophenol
22
min
MN Appl. No. 250060
Analysis of a PCB mixture (Aroclor-1248) on a OPTIMA delta-3 Substances: trichlorobiphenyl; tetrachlorobiphenyl Column: OPTIMA delta-3; 50 m x 0.20 mm ID; 0.20 m Film Phase: OPTIMA delta-3 Cat #: 726400.50 Conditions: Injection volume: 1.0 l Split: 1:25 Carrier gas: He, 25 cm/s Temperature: 100 C --> 220 C, 12 C/min --> 260 C, 1 C/min Detection: MSD
Legend: PCB-31 PCB-28 PCB-52 PCB-49 PCB-44 PCB-61 PCB-70 PCB-66 Cat. #
726410.20 726400.25 726420.60 726442.30 Optima -3 Optima -3 Optima -3 Optima -3
2,4,5-Trichlorobiphenyl 2,4,4-Trichlorobiphenyl 2,2,5,5-Tetrachlorobiphenyl 2,2,4,5-Tetrachlorobiphenyl 2,2,3,5-Tetrachlorobiphenyl 2,3,4,5-Tetrachlorobiphenyl 2,3,4,5-Tetrachlorobiphenyl 2,3,4,4-Tetrachlorobiphenyl Length

20m 25m 60m 30m
Description
ID mm
01mm 0.2mm 0.25mm 0.32mm
Film Thickness
0.10m 0.20m 0.25m 1.00m
max temp 340/360 C 340/360 C 340/360 C 340/360 C
92
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OPTIMA -6 - polysiloxane phase with autoselectivity

Medium polar without CN groups Analytes determine the polarity of the phase Unique, no similar phase Ideal for MSD and PND detectors Max. temperature for isothermal operation 340 C Max. temperature for short isotherms in a temperature program 360 C
Autoselectivity resulting in a wide range of polarities from approximately the non polar OPTIMA 17 to the midpolar OPTIMA 210
Cat. #
726470.60 726480.30 726482.60 Optima -6 Optima -6 Optima -6
Description
ID mm
0.25mm 0.32mm 0.32mm
Length
60m 30m 60m
Film Thickness
0.25m 0.25m 1.00m
max temp 340/360 C 340/360 C 340/360 C max temp 320/340 C 300/320 C 280/300 C 280/300 C 300/320 C 260/280 C 260/280 C 260/280 C 250/260 C 250/260 C 000/000 C 000/000 C 93
Also available
Cat. #
726065.25 726771.60 726787.30 726786.30 726318.50 726874.50 726352.25 726089.60 726296.50 726341.60 723379.25 723384.25 Optima 17 Optima 1301 Optima 624 Optima 624 LB Optima 1701 Optima 210 Optima 225 Optima 240 Optima WAX Optima FFAP FS-Lipodex G (for enantiomer separation) FS-Hydrodex B-TBDAc (for enantiomer separation)
Description
ID mm
0.2mm 0.25mm 0.32mm 0.32mm 0.32mm 0.25mm 0.32mm 0.25mm 0.32mm 0.32mm 0.25mm 0.25mm
Length
25m 60m 30m 30m 50m 50m 25m 60m 50m 60m 25m 25m
Film Thickness
0.2m 0.25m 1.8m 1.8m 0.25m 0.5m 0.25m 0.25m 0.5m 0.25m proprietary proprietary
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Laboratory Equipment
The Centrifan PE - Personal Evaporator
The Centrifan Personal Evaporator (PE) is a portable, easy-to-use centrifugal concentration/evaporation system that provides safe, rapid drying of purified fractions and lab scale reaction mixtures. Operators simply load samples, set the temperature, and close the lid to start the rotor no complex programming is required. The low maintenance system is ideal for drying medicinal chemistry scaffold modifications in drug discovery as well as the reaction mixtures of many other synthetic chemistry applications. The compact unit is also very effective for sample prep evaporation steps and for drying purified lab scale fractions from a Flash LC or HPLC. With a small 8 x 8 inch footprint and only 18 inches high, the self-contained Centrifan PE requires little bench space and doesnt need to be placed under a fume hood. The portable system can also be operated on a lab cart, providing further convenience and savings of bench space. The Centrifan PE provides rotor options including six 20 mL scintillation vials, eight 1.6 mL Eppendorf tubes, and eight 1.8 mL HPLC injection vials. The Centrifan PE Personal Evaporation System is the perfect companion to your RotorVap. It allows for efficient and secure drying of your RotorVap samples or dry pooling of Flash LC fractions in 20 mL scintillation vials. The Centrifan PE is compatible with all organic and aqueous solvents and is great as a standalone system or working in tandem with your RotorVap. The typical final step of drying your RotorVap samples into 20mL scintillation vials puts your valuable samples at risk of loss from bumping and requires personnel to attach a vacuum and monitor RotorVap equipment for the last hour of the drying process. If not done properly, your sample will be ejected from the scintillation vial and smeared across all the interior surfaces of the RotorVap. The Centrifan PE eliminates this risk. The Centrifan PE offers unattended simplicity, sample safety, convenience, and having a closed system to assure material containment and solvent recovery. The Centrifan PE Personal Evaporator combines the features of a centrifuge and a blow-down evaporator, offering unparalleled performance in a simple, workhorse system: High Purity and Yield: centrifugal force ensures sample integrity Easy-to-Use: close the lid to start Works with all Solvents Green Solvent Recovery: gas re-circulates in a closed system Versatile: ideal for drying Flash LC and SPE fractions, medicinal chemistry and other compound synthesis, drying high boilers like DMF and DMSO, cold, inert drying of proteins, strong acids and other inorganic chemistry, and radioisotopes Compact: portable, minimal bench space, small footprint No Vacuum Pump: no sample bumping risk, no maintenance Affordable: pays for itself in 4 months doing final drying step of RotorVap samples Its inherent simplicity means it will run for many years with no maintenance. Its size is ideal for medicinal and other synthetic chemists who need to evaporate small quantities of reaction mixtures. It is also appropriately sized for drying HPLC and Flash LC fractions of interest. The instrument provides a safe, reliable, and green alternative to other evaporators which may not recover the solvents that they evaporate.
How It Works
The Centrifan PE generates its own gas flow to accelerate sample evaporation by using a novel process which recirculates a captured volume of gas. Centrifugal force keeps samples secure in their containers and ensures maximum purity and yield. The technique eliminates the need for a vacuum pump or a large supply of blow down gas, significantly reducing complexity and maintenance compared to rotary evaporators, vacuum centrifuges, and blow-down equipment. Because it operates without a vacuum, the system eliminates the potential for cross contamination and sample loss caused by solvent bumping and foaming. The re-circulating gas flow is produced by a centrifugal fan into which a holder is placed to carry the drying containers. The spinning fan generates a high flow rate of drying gas which is directed through the containers. The rotation generates centrifugal force to keep 100% of the solute pressed in the sample containers, thus preventing compound loss and cross contamination. Evaporated solvent vapors are continuously circulated through a dry ice cold trap to condense the solvent vapor and return dry gas to the Centrifan housing to pick up more solvent. The system achieves very high solvent recovery because the vapor-carrying gas circulates continuously through the cold trap during the drying process. (See the Solvent Tables on the following page to determine drying times)
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Benefits of Using the Centrifan Personal Evaporator vs. Rotovap

Reducing Operator Time for Rotary Evaporators
Problem: Rotor-Vacuum Evaporators are commonly used by synthetic and purification chemists to dry solutions prior to redissolution in another solvent or prior to long term storage of the finished product. Since the volume of the starting solution is always greater than that of the final dried compound, the practice of moving to a 20 mL scintillation vial for the final evaporation step is common. Rotorvap manufacturers have facilitated compound transfer from round bottom flasks to scintillation vials by providing special adapters to maintain a vacuumtight seal between the relatively small vial format and the large vacuum taper of the rotorvap. Drying the last 10mL in a scintillation vial requires care and experience to avoid sample loss or contamination from bumping and foaming. This compound finishing step is critical to the process and can require the same amount of rotorvap time to complete as the first 490 mL, which requires much more of the analysts time. Solution: A new product called the Centrifan PE (for personal evaporator) can be used instead of the rotorvap to efficiently dry the last 10 mL of product solution in a 20 mL scintillation vial with confidence and unattended operation. The Centrifan PE is a portable, easy-to-use centrifugal evaporation system that provides safe, rapid drying of purified fractions and lab scale reaction mixtures. After transferring the 10 mL aliquot from the round-bottom to the scintillation vial, the user simply places the sample in the rotor of the Centrifan PE, closes the lid to start the rotor, and sets the temperature. The solution in the scintillation vial dries securely in the Centrifan PE without further monitoring and frees the laboratory worker for other tasks. In addition, the Centrifan PE offloads the time consuming finishing tasks from the rotorvap thus potentially increasing overall evaporation productivity. The Centrifan PE has streamlined and simplified our workflow through unattended, convenient, and safe processing of the final dry-down step of purified material before returning to the client. --Paul Lefebvre, Laboratory Director Averica Discovery Services, Inc. Worcester, MA USA Solvent Table for 6 x 20mLScint. Vial Solvent Table for 8 x 1.6mL Eppendorf
Solvent
Methanol Hexane Acetone Isopropyl Alcohol DMF Ethyl Acetate AcN/H20 (70/30)
Cat. # MSFC21070 MSFC21080 MSFC21000 MSFC21020 MSFC21110 MSFC21120
Total Volume in Rotor

6 x 10 mL 5 x 5 mL 6 x 10 mL 6 x 10 mL 6 x 10 mL 6 x 10 mL 6 x 10 mL
Description
Temp(C)
40 40 40 40 40 40 40 40
Time to Dry Rotor

60 min 3 hrs 15 min 35 min 70 min 12 hrs 25 min 3 hrs
Solvent
Methanol Hexane Acetone Isopropyl Alcohol DMF Ethyl Acetate AcN/H20 (70/30)
Total Volume in Rotor

8 x 1 mL 8 x 1 mL 8 x 1 mL 8 x 1 mL 8 x 1 mL 8 x 1 mL 8 x 1 mL
Temp(C)
40 40 40 40 40 40 40 40
Time to Dry Rotor

65 min 15 min 20 min 50 min 4 hrs 18 min 35 min 3 hrs
Methylene Chloride 6 x 10 mL
Methylene Chloride 8 x 1 mL
Centrifan PE Personal Evaporator (w/ Cold Trap & Timer), 120 VAC, rotor sold separately Centrifan PE Personal Evaporator (w/ Cold Trap & Timer), 220 VAC, rotor sold separately Centrifan PE Personal Evaporator (w/ Cold Trap), 120 VAC, rotor sold separately Centrifan PE Personal Evaporator (w/ Cold Trap), 220 VAC, rotor sold separately Centrifan PE-L Personal Evaporator Lite (w/o cold trap), 120 VAC, rotor sold separately Centrifan PE-L Personal Evaporator Lite (w/o cold trap), 220 VAC, rotor sold separately
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Rotors and Accessories for the Centrifan PE - Personal Evaporator
MSFC21050 & 21040
MSFC21180
MSFC21030
MSFC21162
MSFC21010
MSFC21011
MSFC21008
MSFC20889
Cat. # MSFC21050 MSFC21180 MSFC21040 MSFC21030 MSFC21162 MSFC21010 MSFC21011 MSFC21008 MSFC21009 MSFC20889
Description
The Centrifan RE - Recirculating Evaporator

The Centrifan RE is a standalone bench top evaporation device that utilizes the gas flow created by the spinning rotor along with heat to provide an incredibly rapid and safe evaporation environment without the need for a vacuum pump. Because the Centrifan RE operates at atmospheric conditions, a heater placed in the recirculating gas-flow is all that is needed to provide precision temperature driven acceleration of the evaporation process. Compared to traditional bench top concentrators that use either a water bath or infrared lamps, Centrifan RE provides a far superior sample-friendly drying environment.
How it Works
The 160 cfm gasflow created by the rotor is redirected through passages to the surface of the liquid inside the evaporation vessel. This aggressive blow drying continuously shears off the top layer of the liquid while the centrifugal force holds the non-volatile compounds securely in place. Tests with talcum powder demonstrated a 0% loss of sample during the evaporation process.
Dry Samples Up to 5 Times Faster No vacuum pump required to dry samples 3 - 5 times faster than benchtop vacuum centrifuges or blow down concentrators.* Save energy, space, and maintenance headaches by eliminating the need for vacuum pumps Eliminate oil mess, heat, noise and vacuum tubing Set temperature up to 60C Programmable heater shut-off time Use storage/media bottles, microtiter plates, or scintillation vials
* All evaporation rates were obtained while connected to a -50C cold trap (cold trap is available separately).
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Operates at Atmospheric Pressure

The Centrifan RE operates at atmospheric pressure and replaces typical vacuum centrifuges or blow down concentrators. Using only heating fins within the rotor compartment and the spinning rotor, the Centrifan RE provides a simple and effective evaporation environment.
Space Saving Design

The compact Centrifan RE can be located anywhere on the lab bench rather than taking up space in a fume hood. Vapors are directed through an exhaust hose that can be connected to the laboratory vent system or directed to a cold trap for condensation. Accommodates Standard Collection Vessels 4 or 8 (250 mL) media bottles 4 (20 x 150 mm) test tubes 24 (13 x 100 mm) test tubes 4 (96 well) microtiter plates 24 (20 mL or 40 mL scintillation vials) Custom rotor configurations are available Specifications Solvents: Aqueous, organic, buffered Dimensions (inches) 20W x 9H x 26D Rotor Speed: Low (800 to 1500 RPM) Temperature Range: Ambient to 60C
The Centrifan RE dries aqueous samples 3X faster than standard vacuum centrifuges and 5X faster than blow down concentrators.
Comparative Performance Specs
Equipment
Vacuum Pump or Compressed Gas Required Container Configuration Temperature Evaporation water 40C Evaporation Menthonal 40C Yes
Standard Vaccum
Standard Blow Down Type

yes Unknown 40C 33L/min 200L/min
Centrifan RA Recirculating Evaporator Centirfuge with Standard (-50C) Cold Trap

no 250 mL media bottle (Filled to 200 mL for rate measurement) 40C 170L/min 812L/min
Unknown 40C 57L/min 125l/min
Cat. # MSFC20250
Description Centrifan RE (120/240V) (price includes one rotor - others priced separately)
Rotors and Accessories for the Centrifan RE - Recirculating Evaporator

Cat. # MSFC20630 MSFC20980 MSFC20650 MSFC20660 MSFC20360 MSFC20760 MSFC20620 MSFC20730 MSFC20640 MSFC20610 MSFC21238 Description Rotor, Centrifan RE, 4 x microtiter plates (.3mL, 1mL, and 2.2mL well volumes OK) Rotor, Centrifan RE, 24 x 20mm x 150mm test tubes (use kits below for other tubes) Kit, Tube Adapter, 24 x 20mm x 150mm rotor able to use 13mm x 100mmtubes Kit, Tube Adapter, 24 x 20mm x 150mm rotor able to use 16mm x 100mm tubes Rotor, Centrifan RE 8 x 250mL bottles (use kit below for smaller bottles) Kit, Bottle Adapter, 8 x 250mL bottle rotor able to use 100mL bottles Rotor, Centrifan RE, 24 x 40mL scintilation vials (use kit below for 20mL scint vials) Adapter Kit, 40mL to 20mL Scintil Vial Cold Trap, Ultra, Labconco (-85) Cold Trap, Std. Labconco (-50) Manual, Centrifan RE
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Centrifuges
Gusto High-Speed Mini-Centrifuge
Maximum speed with minimal space. This compact centrifuge is ideal for most protocols calling for fast spins (up to 12,500 rpm / 9,100 rcf ) and quick spindowns. Useful for PCR , microfilter cell separation, clinical applications, and HPLC protocols. Operation is extremely simple for this highlevel of sophistication: Insert tubes, close lid, adjust speed/time, and press Start. For duplicate spins, simply reload rotor and push Start. Designed with quick-spin option for commonly used 20-second spins. Also includes a tool-free, quick-change rotor system. Safety features ensure the unit will not operate with an open lid, a jammed rotor or when out of balance. Gusto provides the option of setting and displaying in rpm or rcf. Efficiently designed for minimum bench top space. Included features typically found on more expensive units.
Two rotors: 12 place standard rotor with lid for 1.5/2 mL tubes 8-place strip rotor for four 0.2 mL tube strips or 32 single tubes Twelve 0.2 mL and twelve 0.5 mL adapters for customized use
Gusto includes:
The Heathrow Advantage
Designed for 1.5/2.0 mL and 0.5/0.2 mL microfuge tubes Variable speed settings 500 12,500 in 100rpm increments Variable timer 1sec 30 minutes in 1 second increments Selectable digital display of speed in Xg and rpm Low noise and vibration Small footprint Universal power adapter accepts worldwide voltages Imbalance and tilt safety cut out Quick spin or user settable rpm to prevent over compaction Tube positions clearly numbered Unit will not operate without lid in place Double insulated construction
Speed: 500 12,500 rpm RCF : 15 9,100 Xg Dimensions: 203 x 171 x 114mm Power: 100 240 VAC, 50/60 with four interchangeable power cords for U.S./Japan/Taiwan, Continental Europe/Israel, Australia, and United Kingdom Two-year warranty
Specifications:
SciLogex Personal MicroCentrifuge

The SciLogex Personal Micro Centrifuge is well-designed, accurate and portable , making it ideal for research purposes . The accuracy and sturdiness of SciLogex microfuges allows them to be used in a multitude of applications, ranging from pelleting DNA and RNA samples to performing enzymatic tests to removing cell debris from cell homogenates, among other tasks.
SPECIFICATIONS:
Max. Speed Increment Max. RCF Increment Rotor Run Time Driving Motor Safety Devices Power Noise leve Dimensions Weigh
15000rpm (500-15000rpm) 100rpm 15100g 100g 2ml x 12 30sec ~ 99min (Pulse & Continuous Operation) Brushless DC motor Door interlock Over-speed detector Over-temperature detector Automatic internal diagnosis Single-phase, AC110V, 60Hz, 3A 56 (dB) L255xD245H140 (mm) 6kg / 13.2lbs
FEATURES
Powerful centrifugal force 15100 g, broad application range User-friendly large LCD display provides all visual information Brushless motor drives quickly and effortlessly accelerates the rotor to set speed Unique cooling design, minimizes rotor heating Fully autoclavable high strength aluminum alloy rotor Compact design High speed, low noise Affordably priced, to maximize your funds Speed/Acceleration switch Quick spin run function Sound-alert function
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SCILOGEX Vortex Mixer

Compact stable design Adapters availale for tubes, plates etc. (sold separately) Continuous or touch operation Electronic speed control for constant speed even during changes in load Steel base, silicone feet for ultra stablility and vibration damping cTUVus approved CE Marked 2 YEAR WARRANTY
Variable Speed Cat. #7620-0011 SPECIFICATIONS Speed range: 0-2500rpm (Variable), 2500rpm (Fixed) Shaking movement: Orbital, 4mm Speed selection: Analog Motor type: Eccentric, oiless bearings Dimensions: 6L x 6W x 7H Weight: 6lbs Voltage: 110Volts 60Hz
Fixed Speed Cat. #7620-0012
Microplate Mixers
SCILOGEX Microplate Mixer, 0-3000rpm The Microplate Mixer is ideal for mixing/shaking 96 well microplates. The mixers high speed and small orbit combine to offer true vortexing action in each well of the microplate for complete and uniform mixing. Long life quiet brushless motor 2 YEAR WARRANTY
Cat. #
77620-0111 Microplate Mixer
Description
Hand-Held Homogenizer
The most lightweight, high-torque homogenizer available! Offering plenty of power and useful features, the PRO200 has a 144 watt, high-torque motor with variable speed adjustment (5,000 to 35,000 rpm), allowing for homogenization in mere seconds and a maximum noise level of only 72 dB. Highly versatile, this homogenizer is able to homogenize samples with volumes as small as 0.03 ml or as large as 1 L*. Convenient, separate ON/OFF switch allows for the slide switch speed control to remain at your ideal homogenizing speed. Can be used as hand-held or post-mounted. * When used with rotor stator generators, ranging in size from 5mm to 20mm in diameter, or with SAFETY SEAL and Sealed Tube (ST) Series homogenizer Chamber Assemblies Includes: variable speed motor, tool kit, instruction manual
* Contact your Product Specialist for details about generators Cat. #

7518-0300 Hand-Held Homogenizer
Description 99
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Variable Speed Rocker

The VSR-50 Variable Speed Rocker is constructed by the durable steel housing system and supports up to 30 pounds of solution while constricting vibration. The rocker is equipped with a 60-minute timer with the hold feature. The unit comes equipped with a 12 x 14 table. The rocker is available with 12x14 or 16x20 angle adjustable table for maximum control. The Variable Speed Rocker allows 5 to 100 rocking motions per minute.
Digital Shaker Linear/Orbital

Orbital Digital Shaker - 2kg/4.4Lbs Capacity and 7.5kg/16.5 Capacity SCILOGEX SK-O180-Pro Orbital Digital Shaker, 2Kg/4.4Lbs Capacity ideal for Culture Flasks, PetriDishers, Erlenmeyer Flasks, bottles and containers. Wide range of platforms options for culture flasks, flasks etc. (Order separately) 0-500RPM shaking speed range Timer or continuous operation 1-1199 minutes timer Independent LCD digital displays for shaking speed and timer function cTUVus approved CE Marked 2 YEAR WARRANTY
SPECIFICATIONS Speed range: 0-500 RPM Orbit diameter: 10mm Maximum load: 2/Kg/4.4Lbs Operating temperature range: +4 - 40C Maximum humidity: 80% Overall dimensions: (w x d x h) 17 x 15 x 12 inches Weight: 20Lbs Electrical supply: 110V, 60Hz, 25W
Cat. #
6320-9110 6318-1000 6418-2000 7520-0010 7520-0020 SciLogex Personal Micro Centrifuge Benchtop Microlitre Centrifuge Variable Speed Rocker
Description
Digital Shaker Linear, 2kg capacity (also available in 7.5kg capacity, Cat. #7520-0211) Digital Shaker Orbital, 2kg capacity (also available in 7.5kg capacity, Cat. #7520-0221)
10-Place Analog Magnetic Hotplate/Stirrers

SCILOGEX MS-H-S10 10-Position Analog Hotplate Magnetic Stirrer, Ambient-120C, 0-1100rpm The MS-H-S10 10-place multiposition hotplate stirrer features a powerful rotary drive providing consistent sample conditions across each position for volumes up to 400 mL. Heat only, stir only, or heat ands stir simultaneously. The stirrer surface is stainless steel with a silicone anti-slip mat which is chemical resistant and easy-to-clean. Electronic speed control for constant speed even during changes in load High magnetic adhesion Long life brushless motor 2 YEAR WARRANTY
Cat. #
7120-0088 7120-0098 10-Place Analog Magnetic Stirrer 10-Place Analog Magnetic Hotplate Stirrer
Description
100
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Analog Round Magnetic Hotplate/ Stirrer

SCILOGEX Analog Hotplate Stirrers, with S/Steel or Porcelain plate, Ambient-340C, 0-1500rpm Electronic speed control for constant speed even during changes in load Low heat temperature stability Long life brushless motor High magnetic adhesion cTUVus approved CE Marked 2 YEAR WARRANTY
Analog Magnetic Stirrer Cat. #7120-0028
Analog Magnetic Hotplate Stirrer Cat. #7120-0038
Analog 7x7 Square Magnetic Hot Plate/Stirrer

Adjustable safety temperature cut-out: safety cut-out within the 100-580 oC temperature range is adjustable. Accurate temperature and speed control: the microprocessor control technology effectively monitors the temperature and speed precision. 7 Analog Square Hotplate Magnetic Stirrer Cat. #7120-2100
7 Analog Square Plate Magnetic Stirrer Cat. #7120-1100
Digital 7x7 Square Magnetic Hotplate/Stirrer

Features: Adjustable safety temperature cut-out: safety cut-out within the 100-580 oC temperature range is adjustable. Accurate temperature and speed control: the microprocessor control technology effectively monitors the temperature and speed precision.
Digital 7x7 Square Magnetic Hot Plate/ Stirrer Cat. #7120-2300
Digital Magnetic Hotplate/Stirrer

SCILOGEX MS-H-Pro Digital Hotplate Stirrers, with S/Steel or Porcelain hotplate, Ambient-340C, 0-1500rpm Heat only, stir only or heat and stir together functions Automatic spin bar retrieval Low temperature stability Stirring and heating functions can be saved in memory PT1000 remote temperature sensor facility (sensor is sold separately click here) Electronic speed control for constant speed even during changes in load Maintenance free brushless motor for long life High magnetic adhesion cTUVus approved CE Marked 2 YEAR WARRANTY
Digital 7x7 Square Magnetic Stirrer Cat. #7120-1300
Digital Magnetic Stirrer Cat. #7120-0048
Digital Magnetic Hotplate Stirrer Cat. #7120-0058
Fax: 770.936.0326
www.sorbtech.com
101
Weigh Dishes - Cuvettes - Petri Dishes

Anti-Static Weigh Dishes
Our plastic disposable weigh dishes are contaminant-free and biologically inert. They are ideal for weighing liquid or powdered samples. The flat-bottom design ensures stability while smooth surface construction provides accurate pour-outs with minimal sample loss. Perfect for static-affected samples. Hexagonal footprint for balance platform stability Contour sides for easy, secure handling Flexible for dependable sample pouring Conventional heating to 200 degrees Fahrenheit Ideal quick-freeze tray
Cat. #
8802-0020 8802-0050 8802-0200 8802-0350
Description
20ml Weighing Dishes 50ml Weighing Dishes 200ml Weighing Dishes 350ml Weighing Dishes 38mm 64mm 102mm 127mm
Top I.D.
25mm 51mm 76mm 89mm
Base I.D.
4000 500 500 500
Quantity
Cuvettes
Sorbent Technologies offers cuvettes in both 1.5ml semi-micro and 3.5ml standard sizes. All cuvettes are sold 100 per tray, 10 trays per case. For convenience, double-seal caps are sold separately. 10mm path length and 45mm high Internal path width of the semi-micro is 4mm All cells made from polystyrene
Cat. #
8901-0001 8901-0002 8901-0003 8901-0004 Cuvette Cap
Description
Standard Cuvette Macro Fluorimeter Cuvette (four sided) Cuvette Semi-Micro 3.5mL Cap 3.5mL 1.5mL
Size
1000 1000 1000 1000
Quantity
Petri Dishes
Sorbent Technologies offers vented Petri dishes in 60 x 15mm and 90 x 15mm. These economical, translucent dishes are precision molded from biomedical grade polystyrene. Cultures are clearly visible without distortion. Dishes are packaged in heavy-wall polyethylene sleeves.
Cat. #
8019-0090 8019-0060
Description
Vented Petri Dishes, Sterile Vented Petri Dishes, Sterile 90 x 15mm 60 x 15mm
Size
500 500
Quantity
www.sorbtech.com
PCR Products
Cat. #3102-1801
Cat. #3102-0801 2 Made of superior quality medical grade polypropylene Almost translucent for better viewing of the contents Ultra thin wall design ensures rapid thermal transfer Easily open and close with one hand Available in domed or flat topped hinged caps
x = color
Individual Tubes
Cat. #
3002-250X 3002-255X 3502-129X 3502-229X
X = color
Description
1000 1000 1000 1000
Qty
Reaction Tube - 0.5ml, PP, Dome Cap, Natural (X=0) Also available in Blue (X=1), Green (X=2), Red (X=3), Yellow (X=4) Reaction Tube - 0.5ml, PP, Flat Cap, Natural (X=0) Also available in Blue (X=1), Green (X=2), Red (X=3), Yellow (X=4) Reaction Tube - 0.2ml, oil free operation, PP, Dome Cap, Contamination Shield, Natural (X=0) Also available in Blue (X=1), Green (X=2), Red (X=3), Yellow (X=4) Reaction Tube - 0.2ml, oil free operations, PP, Flat Cap, Contamination Shield, Natural (X=0) Also available in Blue (X=1), Green (X=2), Red (X=3), Yellow (X=4)
Strip Tubes
Cat. #
3102-080X 3102-180X 3102-280X 3102-108X 3102-008X
X = color
Description
125 125 125 125 125
Qty
Tube Strip - 0.2 mL tube, strip of 8 tubes, Natural (X=0) Also available in Blue (X=1), Green (X=2), Red (X=3), Yellow (X=4) Domed Strip Cap - strip of 8 caps, for use with #3102-080X, Natural (X=0) Also available in Blue (X=1), Green (X=2), Red (X=3), Yellow (X=4) Flat Strip Cap - Strip of 8 caps, for use with #3102-080X, Natural (X=0) Also available in Blue (X=1), Green (X=2), Red (X=3), Yellow (X=4) PCR Strip 0.2 mL tube, w/ attached Dome cap, strip of 8, Natural (X=0) Also available in Blue (X=1), Green (X=2), Red (X=3), Yellow (X=4) PCR Strip 0.2 mL tube, w/ attached Flat cap, strip of 8, Natural (X=0) Also available in Blue (X=1), Green (X=2), Red (X=3), Yellow (X=4)
Plates and Sealing Film

Cat. #
3402-096X 3402-396X 3402-496X 3402-3840 3302-1000 3302-0500
X = color
Description
10 10 10 10 100
Qty
96-well Flat PCR Plate - Thin Wall, PP, 0.2 mL well capacity, Natural (X=0) Also available in Blue (X=1), Green (X=2), Red (X=3), Yellow (X=4) 96-well Full Skirted PCR Plate - Thin Wall, PP, 0.2 mL well capacity, Natural (X=0) Also available in Blue (X=1), Green (X=2), Red (X=3), Yellow (X=4) 96-well High Chimney PCR Plate - Thin Wall, PP, 0.2 mL well capacity, Natural (X=0) Also available in Blue (X=1), Green (X=2), Red (X=3), Yellow (X=4) 384-well Full Skirted PCR Plate - Thin Wall, PP, 40 mL well capacity, Natural (X=0) Thermal Adhesive Sealing Film - Non-sterile or Sterile Transparent, function from -70oC to +95oC Aluminum Sealing Foil - function from -85oC to +95oC
100 Sheets
Fax: 770.936.0326
www.sorbtech.com
103
Pipet Tips
RNase-free, DNase-free, and Non-pyrogenic
Tips made from 100% virgin polypropylene Bulk bags have reclosable ziplock seal Reloadables consume less storage space than standard racked tips Large footprint eliminates tipping problem with multichannel pipettor Quick and easy to remove or install
Description
Bulk Bag of 1000
Racks 10 Racks of 96
5110-0010
Sterilized 10 Racks of 96
5310-0010
Stacks 2 x 5 Layers of 96
Reloads 10 inserts of 96
5210-0010
0.1-10L Natural Tip Universal Fit
5010-0010
5810-0010
1-200L Natural Tip Graduated Universal Fit
5010-0200
5110-0200
5310-0200
5810-0200
5210-0200
1-200L Yellow Tip Graduated Universal Fit
5010-0202
5110-0202
5310-0202
5810-0202
5210-0202
1-300L Natural Tip Universal Fit
5010-0300
5110-0300
5310-0300
5810-0300
N/A
101-1000L Blue Tip Universal Fit
5010-1001
5110-1001
5310-1001
N/A
N/A
101-1000L Natural Tip Universal Fit
5010-1000
5110-1000
5310-1000
N/A
N/A
0.1-10L Aerosol Barrier Tip Natural Universal Fit, 10 racks of 96, (P-2-10) 1-30L Aerosol Barrier Tip Natural Universal Fit, 10 racks of 96, (P-20) 1-100L Aerosol Barrier Tip Natural Universal Fit, 10 racks of 96, (P-100) 1-200L Aerosol Barrier Tip Natural Universal Fit, 10 racks of 96 101-1000L Aerosol Barrier Tip Natural Universal Fit, 10 racks of 100
5510-0010
5510-0030
5510-0100
5510-0200
5510-1000
www.sorbtech.com
Pipettors
Variable Volume Single and Multi-Channel Pipettors
Smooth plunger action, easy maintenance and calibration, tools supplied Slim line tip ejector allows pipetting into narrow tubes Compatible with a wide range of tips High degree of accuracy and precision
Cat. #
6820-0001 6820-0002 6820-0003 6820-0004 6820-0005 6820-0006 6820-0007 6820-0008 6120-0802 6120-0804 6120-0806 6120-1202 6120-1204 6120-1206
Description
Single Channel Pipettor Single Channel Pipettor Single Channel Pipettor Single Channel Pipettor Single Channel Pipettor Single Channel Pipettor Single Channel Pipettor Single Channel Pipettor 8 - Channel Pipettor 8 - Channel Pipettor 8 - Channel Pipettor 12 - Channel Pipettor 12 - Channel Pipettor 12 - Channel Pipettor
Vol. Range (l)

0.1 - 2.5 0.5 - 10 2 - 20 5 - 50 10 - 100 20 - 200 100 - 1000 1000 - 5000 0.5 - 10 5 - 50 50 - 300 0.5 - 10 5 - 50 50 - 300
Cat. #
6920-1000
Description
Motorized Pipette Filler One-handed operation, Ergonomic Design, LCD Display, Rechargeable Lithium-ion battery Volume: 0.1 - 100ml
6920-000X (X = Color)
Manual Pipette Filler Precision Control, Fits wide range of graduated and volumetric pipettes, Fits both glass and plastic, easy to clean, available in 5 color choices Also available in Blue (X=1), Green (X=2), Red (X=3), Yellow (X=4), and Pink (x=8) Volume: 0.1 - 100ml
Motorized Pipette Filler Manual Pipette Filler
Serological Pipets
Sorbent Technologies now offers serological pipets for transferring liquids and suspensions with a variety of different viscosities. All pipets are virgin polystyrene, certified pyrogen-free and non-cytotoxic. The innovative, non-toxic color-coded cotton plugs provide easy size identification. Unique smooth one-piece construction eliminates sample hang-up and accidental breakage Superior accuracy: 1.5% Standard sterile packaging is one-side-paper one-side-plastic, static-free, easy to open
Cat. #
7317-0010 7317-0020 7317-0050 7317-0100 7317-0250 7317-0500
Description
1ml, Individually Wrapped, Sterile 2ml, Individually Wrapped, Sterile 5ml, Individually Wrapped, Sterile 10ml, Individually Wrapped, Sterile 25ml, Individually Wrapped, Sterile 50ml, Individually Wrapped, Sterile
Length
10.67 10.67 11.57 11.57 11.67 13.38
Grad. Int.
0.01ml 0.02ml 0.10ml 0.10ml 0.20ml 1.00ml
Neg. Grad.
0.3ml 0.4ml 3.0ml 3.0ml 2.6ml 5.0ml
Tip I.D.
1.3 - 1.8mm 1.8 - 2.2mm 1.4 - 2.0mm 1.6 - 2.4mm 2.3 - 3.5mm 3.1 - 3.3mm 500 500 200 200 200 100
Qty
Fax: 770.936.0326
www.sorbtech.com
105
Tubes
Microcentrifuge Tubes
RNase-free, DNase-free, and Non-pyrogenic
Polypropylene tubes with external graduations Writing surface on flat cap and sidewall for quick sample ID Tubes tolerate centrifugation of up to 17,000 xg RCF. Withstands temperatures from +121oC to -80oC Available in Natural, Blue, Yellow, Green, Red, and Sterile
Cat. #
2010-065X 2210-0650 2010-170X 2210-170X-2 2010-200X 2210-200X-2
X = color
Description
0.65ml Graduated Tube - Flat cap, PP, Natural (X=0) Also available in Blue (X=1), Green (X=2), Red (X=3), Yellow (X=4), and Sterile 0.65ml Graduated Tube - Flat Top, PP, Natural, Low Adhesion Surface 1.7ml Graduated Tube - Flat Top, PP, Natural (X=0) Also available in Blue (X=1), Green (X=2), Red (X=3), Yellow (X=4), and Assorted (X=7) 1.7ml Graduated Tube - Flat Top, PP, Natural (0) Low Adhesion Surface Also available in Blue (X=1), Green (X=2), Red (X=3), Yellow (X=4), and Assorted (X=7) 2.0ml Graduated Tube - Flat Top, PP, Natural (X=0) Also available in Blue (X=1), Green (X=2), Red (X=3), Yellow (X=4), and Assorted (X=7) 2.0ml Graduated Tube - Flat Top, PP, Natural (X=0) Low Adhesion Surface
Quantity
1000 500 500 500 400 400
50 ml Tubes
Made from premium polypropylene for excellent chemical resistance. Withstands temperature extremes of -40oC to 125oC High centrifugation of 6500 RCF Exceptional clarity
Cat. #
2419-0050 2419-1050
Description
50ml Tube - PP w/writing surface and cap Bulk bag 50ml Tube - PP w/writing surface and cap / Sterile in 20 trays of 25 500 500
Quantity
15 ml Tubes
Available in polystyrene or polypropylene Polystyrene offers glass-like clarity and can withstand 1800 RCF. Polypropylene has excellent chemical resistance and can withstand 6400 RCF.
Cat. #
2519-0140 2501-1130 2519-1230
Description
15ml Tube - PP w/writing surface and cap Bulk bag 15ml Tube - PS w/writing surface and cap / Sterile in 10 trays of 50 15ml Tube - PP w/writing surface and cap / Sterile in 10 trays of 50 500 500 500
Quantity
www.sorbtech.com
Gloves and Racks

Nitrile Gloves - Powder-Free
100% Latex-free Textured finish / 4-5 mil thickness 9.5 length Powder-free Superior puncture and chemical resistance Cobalt blue Ambidextrous
Cat. #
1012-2000CS 1012-3000CS 1012-4000CS 1012-5000CS Small, 100 per box Medium, 100 per box Large, 100 per box X-Large, 100 per box
Size
Qty/Case
10 boxes 10 boxes 10 boxes 10 boxes
Cat. #
1012-2000 1012-3000 1012-4000 1012-5000 Small, 100 per box Medium, 100 per box Large, 100 per box X-Large, 100 per box
Size
Qty/Box
100 100 100 100
Latex Gloves - Powder-Free

Powder-free Micro-textured satin Proprietary on-line chlorination process 9.5 length Low protein 510k Ambidextrous
Cat. #
1112-1000CS 1112-2000CS 1112-3000CS 1112-4000CS 1112-5000CS X-Small, 100 per box Small, 100 per box Medium, 100 per box Large, 100 per box X-Large, 100 per box
Size
Qty/Case
10 boxes 10 boxes 10 boxes 10 boxes 10 boxes
Cat. #
1112-1000 1112-2000 1112-3000 1112-4000 1112-5000 X-Small, 100 per box Small, 100 per box Medium, 100 per box Large, 100 per box X-Large, 100 per box
Size
Qty/Box
100 100 100 100 100
Racks
Made of highly resistant acetal polymer Shatter-proof, stain-resistant Compact, lightweight, and stackable Ideal for incubators, refrigerators, freezers, and bench tops Available in five distinctive colors
50 mL Cat. #
4002-600X-2 4002-160X-2
15 mL
X=color
Description
Rack - For 15 mL tubes, 60 tube capacity, 3 tiers, stackable Also available in Blue (X=1), Green (X=2), Yellow (X=4), Orange (X=6), and Lilac (X=8) Rack - For 50 mL tubes, 18 tube capacity, 3 tiers, stackable Also available in Blue (X=1), Green (X=2), Yellow (X=4), Orange (X=6), and Lilac (X=8) 2 2
Qty
Fax: 770.936.0326
www.sorbtech.com
107
Racks - Boxes - Burette - Dispenser

Sorbtech freezer racks are made of high quality corrosion resistant stainless steel. Our wide selection of standard sizes for 2 and 3 cardboard boxes are available with handles, sliding drawers, optional removable locking rods, and other features. Contact your product specialist to choose a configuration that suits your labs needs.
Chest Freezer Racks, Racks for Plastic Boxes, as well as many other variations are also available. Please contact a Product Specialist today!
100-Well Storage Racks

Racks feature durable three point hinges and a clasp that locks for secure lid closure. They hold 1.5 - 2.0mL microtubes and cryovials. Feature molded grid lines on the lid, imprinted coordinates on the base, and imprinted and raised coordinates on the box. These are very user-friendly racks! Available in natural or assorted colors (blue, green, purple, yellow, and orange). Packs of 5.
Cardboard Storage Boxes Cardboard freezer boxes. 2 & 3 in.
Cat. #
4303-100X 4303-810X 4514-2081-5 4514-3081-5
X=color
Description
100-Well Micro-tube Plastic Storage Rack - 5 1/2 x 5 1/2 Hinged Lid with clasp that locks for secure lid closure. Holds 1.5 - 2.0 mL micro-tubes. Natural (X=0), Assorted (X=7) 81-Well Freezer Plastic Storage Box - Box fits in standard 5 1/4 freezer modules and holds 1.5 - 2.0 mL micro-tubes. Natural (X=0), Blue (X=1), Green (X=2), Pink (X=4), Assorted (X=7) 2 Cardboard Freezer Boxes with 81 cell divider, 5 1/4 x 5 1/4 3 Cardboard Freezer Boxes with 81 cell divider, 5 1/4 x 5 1/4 5 5 5 5
Qty
Digital Burette
Accuracy within +/- 0.2%, Precision better than 0.1% Resolution 0.01mL Continuous display of volume dispensed with zero reset feature for multiple titrations Simple user calibration and factory reset for ISO 9000 & GLP requirements Visible borosilicate glass barrel protected with a transparent polypropylene sleeve allows inspection of liquid before dispensing Safe anti-tip amber glass reservoirs with slide filler neck Chemically resistant and autoclavable liquid pathaway Bubble-free dispensing
Dispenser
Fully autoclavable at 121 Available in four ranges: 0.5-50.0ml, 1.0-10.0ml, 2.5-25.0ml, 5.0-50.0ml Quick and precise volume adjustment Easy to clean and maintain, tool supplied Chemical resistant to most aggressive liquids and acids (click here to view/ download our chemical compatibility chart) Comes with adapters (25, 28, 32, 38 & 40mm) to fit all common lab bottles 3 YEAR WARRANTY
Cat. # 6607-1030
Cat. # 6620-0505
108
Fax: 770.936.0326
www.sorbtech.com
Microscopes
i4
The i4 modular series microscope features exceptional optical quality and expandability for top-notch performance in the lab. Use the rotating head to adjust the height for taller users. Infinity Plan and Semi-Plan optics with a 30-year anti-fungal coating match quality with value within any clinics budget. The i4 microscope provides comfort, durability, dependability and superior imaging for the lifetime of the laboratory. Accessories include: blue and green filters, dust cover, immersion oil, manual and warranty card.
Head
Binocular (Seidentopf ), Trinoc available Diopter adjustment Inclined 30, rotates 360 10X/18 WF Eyepieces (10X/20X Super WF HP eyepieces available) Interpupillary distance range 55-75mm
Nosepiece
Reverse quadruple nosepiece Multiple ball bearing mounted
Objectives
Infinity Semi-Plan or Plan objectives 4X, 10X, 40XR, 100XR (oil) Anti-fungal, parfocal, parcentric, color-coded
Stage
Mechanical stage (140mm X 140mm) Coaxial drive controls, metal rack & pinion Range of traverse: 73mm x 43mm Stage locking lever Slow-close hydraulic slide finger
Illumination
Moveable Abbe condenser, NA 1.25 Iris Diaphram LED daylight long-life light source Variable light adjustment: 0-20W output 90-240V / 50-60Hz automatic-switching power cord
Focus
Coarse adjustment: range of 30mm Fine adjustment: graduation of 2m Tension control knob
Weight and Dimensions

Height: 14.1 (352.5 mm) Length: 10.7 (267.5 mm) Width: 6.1 (152.5 mm) Weight: 23.0 lbs. (10.43 kg)
Options
Phase contrast, 50x oil objective, Koehler illumination, camera attachments and more
i5
The i5 modular series microscope features the same exceptional qualities as the i4 but offers the diversity of 5 Infinity Plan objectives and has upgraded 10X/20X Super WF HP eyepieces. Available with either a Binoc or Trinoc head in 4X-10X-20X-40XR-100XR (oil) Infinity Plan objectives. Larger stage also available
Cat. #
Description
Binoc head, 4-10-40-100 Infinity Plan objectives Trinoc head, 4-10-40-100 Infinity Plan objectives Binoc head, 4-10-40-100 Infinity Semi Plan objectives Trinoc head, 4-10-40-100 Infinity Semi Plan objectives Binoc head, 4-10-20-40-100 Infinity Plan objectives Trinoc head, 4-10-20-40-100 Infinity Plan objectives Anti-Fungal Coating on All Lenses LED daylight long life illumination
i4 microscope
I4M-B04A-IPL3 I4M-T04A-IPL3 I4M-B04A-ISL3 I4M-T04A-ISL3
i5 microscope
I4M-B05A-IPL3 I4M-T05A-IPL3 Medical-Grade Microscope
Fax: 770.936.0326
www.sorbtech.com
109
Microscopes
Mi5
Sorbtech is proud to introduce the new Mi5 microscope, our most advanced modular microscope to date. The Mi5 was developed for use in a broad range of applications from routine research and lab inspections, to more specific healthcare applications such as pathology, cytology, oncology, hematology, and gynecology. Bright white LED illumination provides clarity and maintains constant color temperature for most specimens. Superior infinity optics and proven, ergonomic design make the Mi5 comfortable to use for extended periods of time. The Mi5 is equipped to ease the burden on the busiest and most discriminating lab technicians and specialists.
Head
Binocular (Seidentopf ), Trinoc available Digital cameras and adapters available Diopter adjustment +/-5 Inclined 30 10X/20X Super WF HP eyepieces Interpupillary distance range 50-75mm Medical-Grade Microscope Anti-Fungal Coating on All Lenses LED daylight Long Life Illumination and Consistent, Even Brightness
Nosepiece
Reverse quintuple nosepiece Multiple ball bearing mounted
Objectives
Infinity Plan objectives 4X, 10X, 20X, 40XR, 100XR (oil) 50X (oil) objective is also available Anti-fungal, parfocal, parcentric, color-coded
Stage
Mechanical stage (135mm X 184mm) Coaxial drive controls, metal rack & pinion Range of traverse: 73mm x 43mm Stage locking lever Slow-close hydraulic slide finger
Focus
Coarse adjustment: range of 22mm Fine adjustment: graduation of 2m Tension control knob
Illumination
Moveable Abbe condenser, NA 1.25 Iris Diaphram LED provides 10,000+ hours of light Variable light adjustment: 0-20W output 90-240V / 50-60Hz automatic-switching power cord
Options
Phase contrast, 50x oil objective, camera attachments, Darkfield, Epi-fluorescence
Weight and Dimensions

Height: 15.6 (396 mm) Length: 16.5 (420 mm) Width: 8.1 (206 mm) Weight: Trinoc: 20.5 lbs. Binoc: 20.0 lbs.
110
Fax: 770.936.0326
www.sorbtech.com
MiniVID
The MiniVID eyepiece camera is available with an RCA output or USB connection. Plug it in to your TV, VCR, or computer. Then, attach it to any microscope for instant video micrography. The innovative MiniVID camera slips into any standard microscope eyepiece tube, in place of the eyepiece or fits onto any standard threaded C-Mount.
MiniVID USB
Image Sensor Max Pixels Interface
USB 2.0 3.1M pixel CMOS chip 2048 X 1536 pixels
MiniVID RCA
Image Sensor Output
5 lux 1/3 inch color CMOS chip 6-foot cord with composite video (RCA)
Sensitivity Signal
NTSC or PAL available
Exposure
Automatic BMP, JPG
Saved Picture File Format System Requirement

Windows 98, XP, or Vista
Power
9V DC < 1amp Includes 110V AC adapter, 220V available
Software
Scopephoto Senior micro-image analysis software
Mounting Requirements
Standard eyetube (23mm and 30mm) Standard C-Mount threading
OmniVID
With upgraded MicroCap software Version 3.0 the OmniVID includes comprehensive measuring capabilities with saved calibrations for multiple microscopes. Connect to any size TV for training large groups, or connect to a PC for capturing digital images or digital video in the easy-to-use MicroCap V2.0 measuring trinocular head with C-Mount threading. The OmniVID camera on an LW Scientific microscope is the ultimate microscopic imaging solution.
Camera
Sensor resolution: 3.1 megapixel Maximum resolution: 8 megapixel LCD screen size: 2 inch LTPS Memory card: SD/MMC card USB interface: Yes TV output: NTSC/PAL Controller: Remote and USB shutter Power Supply: DC 12V 1.5A Picture Format: JPEG Video Format: MPEG4 Printer support: PictBridge and DPOF Mount: C-Mount Optional stage micrometer available for calibrating multiple microscopes
Software
MicroCap V2.0
Accessories
TV out Cable USB Cable Power Adapter USB Shutter Cable Remote Controller SDG Card
Cat. #
Description
MiniVID USB eyepiece / c-mount video camera MiniVID RCA eyepiece video camera Omni-VID C-mount camera with built-in screen
MiniVD
MVC-U3MP-EMT3 MVC-RN77-EMT1
OmniVID
OVC-8MP2-CMT3
Fax: 770.936.0326
www.sorbtech.com
111
Visit us online at www.sorbtech.com
SORBTECH
5955 Peachtree Corners East, Suite A Norcross, GA 30071 Main: 770.936.0323 Tel: 866.SORBTEC Fax: 770.936.0326

Sorbtech Seperation Guide

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SORBTECH

Chromatography and Laboratory Products

Bulk Sorbents Polymeric Resins TLC Plates

SPE Cartridges GC Columns Syringe Filters

Flash Cartridges & Systems FPLC Columns HPLC & UHPLC

Laboratory Equipment Solvent Evaporation Plasticware

Ordering - Sales, Terms, & Conditions

Pricing and Discounts:

Providing Purity and Performance, Service and Selection

Sorbtech Chromatography ....................................................................................... 4 Silica Gel ........................................................................................................................................ 5

Page Laboratory Equipment................................................................ 94

Sorbent Technologies, Inc.

Toll Free: 866.SORBTEC

Non to Low Polarity

Low to Medium Polarity

Medium to High Polarity

100 200 300

500 800 1000

Advantages of Premium Rf Flash Silica Gel

2. Small pore volume: Higher bulk density.

3. Narrow pore size distribution: Minimum small pores.

4. Purity: 99.95% SiO2

Sorbent Technologies, Inc.

Toll Free: 866.SORBTEC

Granular Silica Gel - MPLC, Flash & Gravity LC Phases

Recommended Applications: Aflatoxins Chloramphenicol Pesticides Steroids Vitamins

Silica Gel - Granular

Water Soluble Matter

Sorbent Technologies, Inc.

Toll Free: 866.SORBTEC

MPLC Silica Gel - Granular

Flash & Gravity Silica Gels - Granular

Silica Gel for ASTM D1319-02a and ISO 3837

Sorbent Technologies, Inc.

Toll Free: 866.SORBTEC

Spherical vs. Granular

Spherical Silica Competitor A Items

Cat. #63700 Granular Silica Unit

100, 75-200m 100, 60-200m Granular

2. Particle Size Distribution

3. Chemical Properties / Impurities 4. Flash Chromatography Test

Spherical Silica showed higher resolution. The reasons are:

5a. Adsorption Curve

Spherical 172 10.61 2.48 241 1.6

Granular 235 11.44 3.50 118 2.1

5b. Elution / Desorption Curve

When using spherical silica gel you will have

Sorbent Technologies, Inc.

Toll Free: 866.SORBTEC

Spherical Silica Gel - HPLC, MPLC, Flash & Gravity LC Phases

Sorbent Technologies, Inc.

Toll Free: 866.SORBTEC

HPLC, MPLC Silica Gel- Spherical

HPLC, Prep HPLC, and MPLC Chromatography Grades - Spherical

20-45m 20-45m 20-45m 20-45m 20-45m 20-45m

Flash & Gravity Silica Gel- Spherical

Flash & Gravity Chromatography Grades - Spherical