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High-Performance Liquid Chromatography (HPLC): Principles, Applications, Versatality, Efficiency, Innovation and Comparative Analysis in Modern Analytical Chemistry and In Pharmaceutical Sciences
Version 1
: Received: 1 September 2024 / Approved: 2 September 2024 / Online: 2 September 2024 (08:54:14 CEST)
How to cite:
Ahmed, R. High-Performance Liquid Chromatography (HPLC): Principles, Applications, Versatality, Efficiency, Innovation and Comparative Analysis in Modern Analytical Chemistry and In Pharmaceutical Sciences. Preprints2024, 2024090057. https://doi.org/10.20944/preprints202409.0057.v1
Ahmed, R. High-Performance Liquid Chromatography (HPLC): Principles, Applications, Versatality, Efficiency, Innovation and Comparative Analysis in Modern Analytical Chemistry and In Pharmaceutical Sciences. Preprints 2024, 2024090057. https://doi.org/10.20944/preprints202409.0057.v1
Ahmed, R. High-Performance Liquid Chromatography (HPLC): Principles, Applications, Versatality, Efficiency, Innovation and Comparative Analysis in Modern Analytical Chemistry and In Pharmaceutical Sciences. Preprints2024, 2024090057. https://doi.org/10.20944/preprints202409.0057.v1
APA Style
Ahmed, R. (2024). High-Performance Liquid Chromatography (HPLC): Principles, Applications, Versatality, Efficiency, Innovation and Comparative Analysis in Modern Analytical Chemistry and In Pharmaceutical Sciences. Preprints. https://doi.org/10.20944/preprints202409.0057.v1
Chicago/Turabian Style
Ahmed, R. 2024 "High-Performance Liquid Chromatography (HPLC): Principles, Applications, Versatality, Efficiency, Innovation and Comparative Analysis in Modern Analytical Chemistry and In Pharmaceutical Sciences" Preprints. https://doi.org/10.20944/preprints202409.0057.v1
Abstract
High-Performance Liquid Chromatography (HPLC) is an advanced analytical technique employed to separate, identify, and quantify components in complex mixtures. The method operates based on the differential affinities of sample molecules for the mobile and stationary phases within a chromatographic column. HPLC is recognized for its high resolution, accuracy, and efficiency, making it indispensable across various fields including pharmaceuticals, biotechnology, environmental analysis, and forensic science. The technique's adaptability to different detectors such as UV-visible, fluorescence, and mass spectrometry enhances its versatility and precision in analyzing a wide array of substances. Despite its advantages, HPLC presents challenges such as high operational costs, the need for specialized expertise, and susceptibility to contamination.Nonetheless, its role in modern analytical chemistry is pivotal, providing crucial insights and supporting developments in diverse scientific and industrial domains. This abstract encapsulates the core aspects of the article, highlighting the significance and applications of HPLC while also acknowledging its limitations.
Public Health and Healthcare, Public Health and Health Services
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.