Search Results (6,582)

A six-week feeding trial was conducted to assess the safety of single-domain antibodies (sdAbs) derived from camelids against the white spot syndrome virus (WSSV) (WSSVvp28 was used as the antigen), focusing on the whole-organism responses and molecular-level changes in juvenile whiteleg shrimp (Litopenaeus vannamei). Five experimental diets with varying levels of sdAbs were formulated: CON (no sdAb supplementation); SDA_8.2 (8.20% of sdAbs); SDA_16.4 (16.40% of sdAbs); SDA_24.6 (24.60% of sdAbs); and SDA_32.8 (32.80% of sdAbs). In the CON diet, 450 mL of water per kg of diet (45%) was used to form a feed dough, while sdAbs were used to replace the water in the treatment diets. A total of 450 shrimp, with an initial body weight of 3.27 ± 0.02 g (mean ± SEM), were randomly distributed in 15 tanks (30 shrimp per tank; three tanks per treatment). Each tank was filled with 30 L of seawater (77 L capacity) in an indoor semi-recirculating system with a constant water flow rate of 1.2 L min⁻¹. The photoperiod was maintained at 12 h of light and 12 h of dark. The water temperature, pH, salinity, and dissolved oxygen were 27.3 ± 0.1 °C, 7.61 ± 0.01, 34 ± 1 ppt, and 5.94 ± 0.04 mg L⁻¹, respectively. During the feeding trial, the shrimp were fed the experimental diet (40% protein and 11% lipid) three times a day for six weeks. Following the feeding trial, an acute cold-water-temperature stress test was conducted by abruptly exposing the shrimp from each treatment to 15 °C for 4 h, down from 27 °C. The results showed no significant differences in the growth performance (weight gain, feed utilization efficiency, survival, etc.), plasma metabolites (aspartate aminotransferase activity, alanine aminotransferase activity, total protein, and glucose), or antioxidant enzymes (superoxide dismutase and glutathione peroxidase) among all the experimental diets (p > 0.05). In the acute cold-temperature stress test, there was no significant interaction between sdAb supplementation and temperature stress, nor any main effect from either factor, except for the main effect of temperature stress on the glucose levels, which was significantly higher in shrimp exposed to cold-temperature stress (p < 0.05). The next-generation sequencing of differentially expressed genes (DEGs) in the hepatopancreases of shrimp fed the CON, SDA_16.4, and SDA_32.8 diets, followed by Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses, indicated that DEGs were significantly enriched in signaling pathways associated with growth, cold stress, and antioxidant systems. Overall, the results from conventional measurements suggest that the use of sdAbs against the WSSV may be safe for juvenile whiteleg shrimp. However, findings from the sophisticated analysis indicate that further research is needed to understand the molecular mechanisms underlying the observed changes, and to evaluate the long-term effects of sdAb supplementation in shrimp diets. Full article

The phenomenon of global climate change has led to an increase in the frequency of extreme precipitation events, an acceleration in the melting of glaciers and snow cover, and an elevation of the risk of flooding. In this study, the DB-IWHR model was employed in conjunction with the MIKE 21 hydrodynamic model to develop a simulation system for the dam failure flow process of an earth and rock dam. The study concentrated on the KET reservoir, and 12 dam failure scenarios were devised based on varying design flood criteria. The impact of reservoir failures on flood-risk areas was subjected to detailed analysis, with consideration given to a range of potential failure scenarios and flood sizes. It was determined that under identical inflow frequency conditions, the higher the water level, the more rapid the breakout process and the corresponding increase in flood peak discharge. Conversely, for a given frequency of incoming water, an elevated water level results in a transient breach process, accompanied by a reduction in flood peak flow. Moreover, for a given water level, an increase in water frequency results in a reduction in breaching time, an extension of flood duration, and an increase in flood peak flow. The observed trend of flood spreading is generally north-south, and this process is highly compatible with the topographic and geomorphological features, demonstrating good adaptability. Full article

Background: The restoration of the degraded sandy grasslands in Hulun Buir is crucial for maintaining the local ecological balance and sustainable development. Caragana microphylla Lam., a shrub species widely employed in the restoration of sandy vegetation. It is essential to understand its impact on the understory vegetation and soil properties during this process. Methods: This study employed ANOVA, Pearson correlation, and redundancy analysis to systematically analyze the impact of C. microphylla on the three critical stages of desertified grassland vegetation recovery: semi-fixed dunes, fixed dunes, and sandy grasslands. It provided strategies for the restoration of desertified grassland vegetation and offered additional theoretical evidence for the role of vegetation in promoting the recovery of sandy lands. Results: (1) As the degree of vegetation recovery in desertified grasslands increases, the species richness of understory vegetation, Shannon–Wiener index, community height, and biomass also increase. Both the community height and biomass within shrublands are higher than outside, with species richness within the shrublands being higher than outside during the semi-fixed and fixed-sand land stages. (2) In both the 0~10 cm and 10~20 cm soil layers, soil water content showed an increasing trend, peaking in the sandy grassland stage (1.2%), and was higher within the shrublands than outside. The soil water content at 10~20 cm was higher than in the 0~10 cm layer. In both layers, clay and silt content gradually increased with the degree of vegetation recovery in the sandy land, and higher within the shrublands than outside, while the opposite was true for sand content. (3) In both soil layers, soil organic carbon gradually increased with the degree of vegetation recovery, peaking in the sandy grassland stage (4.12 g·kg⁻¹), and was higher within the shrublands than outside. Total nitrogen increased from the semi-fixed-sand land stage to the fixed-sand land stage, with higher levels within the shrublands than outside at all stages. Soil pH within the shrublands decreased as the degree of vegetation recovery increased. There was no significant change in the total phosphorus content. (4) In both soil layers, soil physicochemical characteristics accounted for 59.6% and 46.9% of the vegetation changes within and outside the shrublands, respectively, with the main influencing factors being the soil particle size, total nitrogen, soil water content, and soil organic carbon. Conclusions: In the process of sandy grassland restoration, C. microphylla facilitates the growth and development of vegetation by enhancing the underlying soil physicochemical properties, specifically regarding the soil particle size distribution, soil water content, soil organic carbon, and total nitrogen. Full article

Ongoing investments in irrigation technologies highlight the need to accurately estimate the longevity and magnitude of water savings at the watershed level to avoid the paradox of irrigation efficiency. This paradox arises when irrigation pumping exceeds crop water demand, leading to excess water that is not recovered by the watershed. Comprehensive water accounting from farm to watershed scales is challenging due to spatial variability and inadequate socio-hydrological data. We hypothesize that water savings are short term, as prior studies show rapid recharge responses to surface changes. Precise estimation of these time scales and water savings can aid water managers making decisions. In this study, we examined water savings at three 65-hectare sites in Nebraska with diverse soil textures, management practices, and groundwater depths. Surface geophysics effectively identified in-field variability in soil water content and water flux. A one-dimensional model showed an average 80% agreement with chloride mass balance estimates of deep drainage. Our findings indicate that groundwater response times are short and water savings are modest (1–3 years; 50–900 mm over 10 years) following a 120 mm/year reduction in pumping. However, sandy soils with shallow groundwater show minimal potential for water savings, suggesting limited effectiveness of irrigation efficiency programs in such regions. Full article

Small-sized fish are a vital food source for large predatory commercial fish and play a key role in marine food webs, bridging lower and higher trophic levels. They are indispensable in maintaining the energy flow and material cycling within aquatic ecosystems. This study utilized bottom-trawl survey data from 2017 to 2020 along the south inshore of Zhejiang, China, complemented by concurrent environmental data, to examine the influence of environmental factors on the resource density and seasonal distribution patterns of four dominant small-sized fish species. The research findings indicated that SSH (sea surface height) and Chl (chlorophyll-a concentration) emerged as the key environmental factors influencing resource densities, with all four species exhibiting similar preferences toward these variables. However, significant disparities were observed in their preferences for SST (sea surface temperature), SSS (sea surface salinity), and DO (dissolved oxygen). The various species’ resource density and distribution patterns underwent significant seasonal variations. Additionally, the seasons and regions with the highest resource densities consistently aligned, occurring predominantly in autumn within the northern waters of the study area. This research further elucidated the environmental predilections and seasonal spatial distribution traits of small-sized fish in the south inshore of Zhejiang, an important feeding ground for economic fish species in the East China Sea. This provides scientific backing for forecasting alterations in coastal fishery resources under environmental and climate change scenarios and supports ecosystem-based fisheries management strategies. Full article

The study was conducted to study the sustainability and enhanced nutrition gains obtained from incorporating grape skin powder (GSP) extracted from both Fetească Neagră and Rară Neagră grape varieties into yogurt. Grape skins are major leftovers from wineries, having high amounts of phenolic compounds and dietary fiber responsible for their ability to improve the characteristics of food. The research aimed to evaluate the effect of GSP addition at varying concentrations (0.5%, 1.0%, and 1.5%) on the yogurt’s physicochemical properties, antioxidant activity, color parameters, and sensory attributes. Analysis revealed that both Fetească Neagră and Rară Neagră GSP increased the total phenolic content and antioxidant activity; however, Fetească Neagră showed greater improvements, with TPC reaching 1.52 mg GAE/100 g and DPPH inhibition up to 26.63%. Although slightly lower, TPC rose to 1.43 mg GAE/100 g and DPPH inhibition increased to 18.93% with Rară Neagră enhancing these parameters conversely. Color changes were observed in fortified yogurts where lightness decreased (L*) and redness increased (a*) due to the pH-dependent anthocyanin stability. Syneresis, indicative of yogurt’s water-holding capacity, was reduced at higher concentrations of GSP from both varieties, suggesting improved textural integrity. Sensory evaluation indicated that consumers generally favored yogurts with lower concentrations of GSP. Yogurts fortified with Fetească Neagră GSP received higher overall preference, while those with Rară Neagră GSP were also well-received for their distinct flavor profiles when used at suitable levels. These results show that GSP from both types of grapes improves the nutritional value of yogurt and complies with the principles of sustainable food production through reutilizing agro-industrial waste. Full article

Lactic acid bacteria (LAB) inoculants are commonly used in silage production, yet their effects on silage containing antimicrobial components, such as those found in Leonurus japonicus, remain less explored. Herein, the harvested alfalfa were thoroughly mixed with dried Leonurus japonicus Houtt. (LJH) at a ratio of 9:1 on a fresh weight basis and treated without (CK) or with a lactic acid bacterial inoculant (L; Lentilactobacillus buchneri). The mixtures were stored under anaerobic conditions in vacuum-sealed polyethylene bags for 30 days at ambient temperature. The L-treated silage exhibited high levels of water-soluble carbohydrates (4.98% dry matter (DM)) and acid detergent fiber (27.88% DM). Compared to that of treatment CK, treatment with L increased the acetic acid content of the silage, as a result of increased (p < 0.05) bacterial dominance and decreased (p < 0.05) bacterial richness indices (e.g., Pielou’s E, Shannon, and Simpson) in the pre-storage period. However, these changes gradually reduced as the storage length increased. Treatment L reshaped the bacterial community structure of silage, by increasing the prevalence of Lactobacillus and reducing relative abundances of Enterococcus and Weissella. However, the principal coordinate and Bray–Curtis index analyses illustrated that samples from the L-treated silages exhibited similarities to the CK samples post-fermentation. Overall, the effect of LJH on LAB was only observed in the later stages of fermentation, which did not sufficiently change the silage quality. Hence, using LJH in silage is vital for clean livestock production without compromising the function of LAB when mixed with alfalfa silage. Full article

The effects of climate change in the forms of rising sea levels and increased frequency of storms and storm surges are being noticed across many coastal communities around the United States. These increases are impacting the timing and frequency of tidal and rainfall influenced compound groundwater flooding events. These types of events can be exemplified by the recent and ongoing occurrence of groundwater flooding within building basements at the historic Strawbery Banke Museum (SBM) living history campus in Portsmouth, New Hampshire. Fresh water and saline groundwater intrusion within basements of historic structures can be destructive to foundations, mortar, joists, fasteners, and the overlaying wood structure. Although this is the case, there appears to be a dearth of research that examines the use of wireless streaming sensor networks to monitor and assess groundwater inundation within historic buildings in near-real time. Within the current study, we designed and deployed a three-sensor latitudinal network at the SBM. This network includes the deployment and remote monitoring of water level sensors in the basements of two historic structures 120 and 240 m from the river, as well as one sensor within the river itself. Groundwater salinity levels were also monitored within the basements of the two historic buildings. Assessments and model results from the recorded sensor data provided evidence of both terrestrial rainfall and tidal influences on the flooding at SBM. Understanding the sources of compound flooding within historic buildings can allow site managers to mitigate better and adapt to the effects of current and future flooding events. Data and results of this work are available via the project’s interactive webpage and through a public touchscreen kiosk interface developed for and deployed within the SBM Rowland Gallery’s “Water Has a Memory” exhibit. Full article

Background/Objectives: Heavy metals are a group of metals and metalloids that have a relatively high density. They can cause toxicity even at very low levels. Trace elements are required by all living organisms to maintain their normal growth, metabolism, and development. Oral intake is the main route of exposure to both heavy metals and trace elements. Phenylketonuria (PKU) is the most common amino acid metabolic disorder, and the best known treatment for patients requiring treatment is a phenylalanine (Phe)-restricted diet. The objective of the present study was to evaluate the plasma heavy metal levels, sources of exposure, changes in these levels according to dietary regimen, and trace element levels and their correlations with heavy metals in PKU patients. Methods: The study was conducted between July 2022 and January 2024 on 105 patients aged 2–6 years diagnosed with PKU. Results: The percentage of Pb levels in individuals in the upper quartile increased by 3.47 times (95% CI = 1.07–11.29) in those who consumed canned foods and 7.29 times (95% CI = 1.21–44.03) in those who consumed spring water. The percentage of As levels in the upper tertile increased by a factor of 7.26 (95% CI = 2.09–25.28) in individuals under four years of age and 8.17 times (95% CI = 2.13–31.27) in canned food users. The odds of having blood Cd levels in the upper tertile were 0.09 (95% CI = 0.01–0.96) for those being breastfed for 6–11 months compared to 0–5 months. Zn levels were lower (93.0 vs. 83.6 µg/dL, p = 0.008) in patients on a Phe-restricted diet. Conclusions: The present study did not find a relationship between heavy metal exposure and the dietary treatment status of patients with PKU. Our findings indicate that canned food consumption is a significant contributing factor to heavy metal exposure in PKU patients. Furthermore, our findings revealed a relationship between age, perception of economic level, breastfeeding, kitchen equipment, and water usage and the levels of certain heavy metals. Full article

As climate change intensifies, the need to conserve ecosystem services and our natural resources increases. Nature-based solutions projects that focus on sequestering carbon can also have significant impacts on the ecosystem services in the project area. Herein, we describe a method to quantify ecosystem services via the Integrated Valuation of Ecosystem Services and Tradeoffs (Version 3.14) model. We use those quantitative methods to show where carbon projects and other restoration projects could increase certain ecosystem services through best practices. Using the Edisto River Basin in South Carolina as a study site, we developed a spatial additionality model that shows where water retention, carbon, and sediment retention can be improved. InVEST modeling showed high levels of sediment export and water yield, with 0.12 tons/acre of sediment exported and 256.3 cm/acre of water yielded downstream on average. The model indicates that over 70,000 acres comprised of parcels greater than 20 contiguous acres could implement management to increase ecosystem service provisioning. This model output shows spatially where best management practices can be implemented to achieve positive outcomes and where carbon projects could be implemented to derive additional co-benefits. Furthermore, it can be used as a tool for measurement and verification as data is updated. Full article

A subpopulation of astrocytes on the brain’s surface, known as subpial astrocytes, constitutes the “glia limitans superficialis” (GLS), which is an interface between the brain parenchyma and the cerebrospinal fluid (CSF) in the subpial space. Changes in connexin-43 (Cx43) and aquaporin-4 (AQP4) proteins in subpial astrocytes were examined in the medial prefrontal cortex at postoperative day 1, 3, 7, 14, and 21 after sham operation and sciatic nerve compression (SNC). In addition, we tested the altered uptake of TRITC-conjugated 3 kDa dextran by reactive subpial astrocytes. Cellular immunofluorescence (IF) detection and image analysis were used to examine changes in Cx43 and AQP4 protein levels, as well as TRITC-conjugated 3 kDa dextran, in subpial astrocytes. The intensity of Cx43-IF was significantly increased, but AQP4-IF decreased in subpial astrocytes of sham- and SNC-operated rats during all survival periods compared to naïve controls. Similarly, the uptake of 3 kDa dextran in the GLS was reduced following both sham and SNC operations. The results suggest that both sciatic nerve injury and peripheral tissue injury alone can induce changes in subpial astrocytes related to the spread of their reactivity across the cortical surface mediated by increased amounts of gap junctions. At the same time, water transport and solute uptake were impaired in subpial astrocytes. Full article

This paper presents a method for estimating reservoir storage capacity using the Gould–Dincer normal formula (G-DN), enhanced by the possibility theory. The G-DN equation is valuable for regional studies of reservoir reliability, particularly under climate change scenarios, using regional statistics. However, because the G-DN formula deals with measured data, it introduces a degree of uncertainty and fuzziness that traditional probability theory struggles to address. Possibility theory, an extension of fuzzy set theory, offers a suitable framework for managing this uncertainty and fuzziness. In this study, the G-DN formula is adapted to incorporate fuzzy logic, and the possibilistic nature of reservoir capacity is translated into a probabilistic framework using α-cuts from the possibility theory. These α-cuts approximate probability confidence intervals with high confidence. Applying the proposed methodology, in the present crisp case with the storage capacity D = 0.75, the value of the capacity C was found to be $1271\times {10}^6 {\mathrm{m}}^3$, and that for D = 0.5 was $634.5\times {10}^6 {\mathrm{m}}^3$. On the other hand, in the fuzzy case using the possibility theory, the value of the capacity for D = 0.75 is the internal $[315,5679]\times {10}^6 {\mathrm{m}}^3$ and for D = 0.5 the value is interval $[158,2839]\times {10}^6 {\mathrm{m}}^3$, with a probability of ≥95% and a risk level of α = 5% for both cases. The proposed approach could be used as a robust tool in the toolkit of engineers working on irrigation, drainage, and water resource projects, supporting informed and effective engineering decisions. Full article

Although consumption is the cornerstone of economic growth, it is unquestionably one of the main reasons for the current degradation of the planet. Hence, it is necessary to understand consumers’ different perceptions and behaviors regarding sustainable goods, as the solution will inevitably involve changing behaviors and promoting more sustainable consumption. In light of the challenges posed by sustainability within the wine sector, namely issues related to climate change, chemical exposure, and the availability of water and energy, and considering the industry’s substantial environmental and social impacts, it is important to understand the factors influencing wine consumer behavior. This research explores sustainable wine consumption in Portugal. Based on a sample of 197 valid responses, collected in an online survey that took place from March 20 to April 30 of 2024, and applying the Theory of Planned Behavior (TPB) framework, the current study found that consumers’ intention to consume sustainable wine is influenced by their attitudes, subjective norms, and sustainability awareness. Regarding the individuals’ consumption of sustainable wine, this behavior is influenced by the intention to consume this wine, and by consumers’ perceived behavioral control. The results also concluded that individuals’ intention to consume sustainable wine is significantly different across marital status, professional occupation, level of education, and age. With these valuable insights, this study aimed to increase the available information on sustainable wine consumption, allowing different stakeholders, namely political decision makers, to develop and implement more efficient measures to promote sustainable wine consumption. Full article

Against the backdrop of climate change, soil loss, and water scarcity, sustainable food production is a pivotal challenge for humanity. As the global population grows and urbanization intensifies, innovative agricultural methods are crucial to meet rising food demand, while mitigating environmental degradation. Hydroponic and aquaponic systems, has emerged as one of these solutions by minimizing land use, reducing water consumption, and enabling year-round crop production in urban areas. This study aimed at assessing the yield, ecophysiological performance, and nutritional content of Lactuca sativa L. and Cichorium endivia L. var. crispum grown in hydroponic and aquaponic floating raft systems, with Oreochromis niloticus L. integrated into the aquaponic system. Both species exhibited higher fresh biomass and canopy/root ratios in hydroponics compared to aquaponics. Additionally, hydroponics increased the leaf number in curly endive by 18%. Ecophysiological parameters, such as the leaf net photosynthesis rate, actual yield of PSII, and linear electron transport rate, were also higher in hydroponics for both species. However, the nutritional profiles varied between the two cultivation systems and between the two species. Given that standard fish feed often lacks sufficient potassium levels for optimal plant growth, potassium supplementation could be a viable strategy to enhance plant development in aquaponic systems. In conclusion, although aquaponic systems may demonstrate lower productivity compared to hydroponics, they offer a more sustainable and potentially healthier product with fewer harmful compounds due to the reduced use of synthetic fertilizers, pesticides, and the absence of chemical residue accumulation. However, careful system management and monitoring are crucial to minimize potential contaminants. Full article

In general, young plants in the establishment phase demonstrate sensitivity to changes in environmental conditions, especially regarding water availability. The effects of the seasonality of biophysical processes on plant physiology can trigger differential responses, even within the same region, making it necessary to conduct studies that characterize the physiological performance of the species at different spatial and temporal scales, making it possible to understand their needs and growth limits under water stress conditions. This paper aimed to evaluate the growth, gas exchange and Chl a fluorescence in ipê-rosa seedlings subjected to levels of water replacement (LWRs) of 100, 75, 50 and 25% in a greenhouse. The morphometric variables of plant height, diameter at stem height, numbers of leaves and leaflets, root length and volume, plant dry mass and leaf area were evaluated. The potential evapotranspiration of seedlings (ETc) was obtained using direct weighing, considering the water replacement of 100% of the mass variation between subsequent days as a reference; the cultivation coefficients (kc) were obtained using the ratio between ETc and the reference evapotranspiration (ETo) obtained by the Penman–Monteith FAO-56 method. Biomass and evapotranspiration data were combined to determine water sensitivity. Diurnal fluxes of gas exchange (net photosynthesis rate, transpiration rate, stomatal conductance, internal and atmospheric carbon ratio, water use efficiency and leaf temperature) and Chl a fluorescence (Fv/Fm, Φ_PSII, ETR, Fv′/Fm′, NPQ and qL) were evaluated. Water restriction caused reductions of 90.9 and 84.7% in the increase in height and diameter of seedlings subjected to 25% water replacement when compared to seedlings with 100% water replacement. In comparison, biomass accumulation was reduced by 96.9%. The kc values increased throughout the seedling production cycle, ranging from 0.59 to 2.86. Maximum water sensitivity occurred at 50% water replacement, with Ky = 1.62. Maximum carbon assimilation rates occurred in the morning, ranging from 6.11 to 12.50 µmol m⁻² s⁻¹. Ipê-rosa seedlings regulate the physiology of growth, gas exchange and Chl a fluorescence depending on the amount of water available, and only 25% of the water replacement in the substrate allows the seedlings to survive. Full article