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15 pages, 832 KiB  
Article
Start Task Crafting, Stay Away from Cyberloafing: The Moderating Role of Supervisor Developmental Feedback
by Man Hai, Xuyao Wu, Bingping Zhou and Ye Li
Behav. Sci. 2024, 14(10), 960; https://doi.org/10.3390/bs14100960 (registering DOI) - 17 Oct 2024
Abstract
Cyberloafing as a production deviance behavior raises organizational concerns. Unfortunately, it is unknown how to minimize cyberloafing from a bottom-up perspective, particularly different types of cyberloafing. This study draws on the job crafting and dual-process theory to construct a framework for understanding the [...] Read more.
Cyberloafing as a production deviance behavior raises organizational concerns. Unfortunately, it is unknown how to minimize cyberloafing from a bottom-up perspective, particularly different types of cyberloafing. This study draws on the job crafting and dual-process theory to construct a framework for understanding the relationship between task crafting and passive–active cyberloafing, as well as their boundary condition (i.e., supervisor developmental feedback). We adopted a convenient sampling method, following a two-stage sampling with a time interval of 2 weeks. A sample of 614 full-time employed adults were recruited from the online survey. The results showed that: (1) Task crafting was negatively related to passive and active cyberloafing, respectively. (2) The impact of task crafting on passive cyberloafing rather than active cyberloafing was moderated by supervisor developmental feedback, such that task crafting had significant negative relations with passive cyberloafing when supervisor developmental feedback was higher (vs. lower). Overall, our research findings indicate that passive cyberloafing seems more sensitive to the organizational environment than active cyberloafing, thus different types of cyberloafing have different intervention strategies. Full article
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<p>Hypothesized model of relationships among task crafting, developmental feedback, passive and active cyberloafing.</p>
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<p>Unstandardized structural path coefficients and standard errors (in parentheses) for the structural equation model. Dashed arrows indicate non-significant effects. * <span class="html-italic">p</span> &lt; 0.05, ** <span class="html-italic">p</span> &lt; 0.01.</p>
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<p>The moderating effect of supervisor development feedback on the relationship between task crafting and passive cyberloafing.</p>
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20 pages, 14818 KiB  
Article
Control of Seepage Characteristics in Loose Sandstone Heap Leaching with Staged Particle Sieving-Out Method
by Quan Jiang, Mingtao Jia, Yihan Yang and Chuanfei Zhang
Minerals 2024, 14(10), 1039; https://doi.org/10.3390/min14101039 (registering DOI) - 17 Oct 2024
Abstract
This paper studies the influence of the staged particle sieving-out method on the seepage characteristics in loose sandstone heap leaching. The staged sieving out of ore sample particles was conducted according to particle size, and ground pressure was applied to them. Subsequently, parameters [...] Read more.
This paper studies the influence of the staged particle sieving-out method on the seepage characteristics in loose sandstone heap leaching. The staged sieving out of ore sample particles was conducted according to particle size, and ground pressure was applied to them. Subsequently, parameters such as the permeability, particle distribution, and pore distribution characteristics of the rock samples were obtained to investigate the influence of the staged particle sieving-out method on the seepage effect of loose sandstone heap leaching. The results indicate that sieving out particles smaller than 0.15 mm can significantly reduce the probability of hole blockage and increase the overall pore size, greatly enhancing permeability. Sieving out particles with sizes between 0.15 mm and 1.2 mm can result in the loss of skeleton particles, reducing the amount of flow channels and thereby decreasing permeability. Sieving out particles larger than 1.2 mm can reduce the overall particle size of rock samples, improve strength and pressure stability, and help maintain permeability. In the surface heap leaching of loose sandstone ore, by sieving out particles smaller than 0.15 mm during deep heap construction and sieving out particles larger than 1.2 mm during mid-level heap construction, and by using vat leaching for sieved-out particles, the seepage effect of the ore heap can be significantly optimized, and complete utilization of resources can be ensured. Full article
(This article belongs to the Section Mineral Processing and Extractive Metallurgy)
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<p>Typical surface heap leaching method and stress analysis of ore heap. (<b>a</b>) Typical structure of a heap leaching field; (<b>b</b>) Stress condition of shallow particles; (<b>c</b>) Stress condition of medium-deep particles; (<b>d</b>) Stress condition of deep particles.</p>
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<p>(<b>a</b>) Particle distribution and (<b>b</b>) pore distribution characteristic curves of the original samples.</p>
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<p>Sample preparation. (<b>a</b>) particle size distribution (<b>b</b>) finished samples.</p>
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<p>Samples after pressurization treatment.</p>
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<p>Depth–permeability relationship. (<b>a</b>) Group A; (<b>b</b>) Group B; (<b>c</b>) Group C; (<b>d</b>) Group D; (<b>e</b>) Group E; (<b>f</b>) Group F.</p>
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<p>Particle distribution characteristics curve. (<b>a</b>) Group A; (<b>b</b>) Group B; (<b>c</b>) Group C; (<b>d</b>) Group D; (<b>e</b>) Group E; (<b>f</b>) Group F.</p>
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<p>Pore distribution characteristics curve. (<b>a</b>) Group A; (<b>b</b>) Group B; (<b>c</b>) Group C; (<b>d</b>) Group D; (<b>e</b>) Group E; (<b>f</b>) Group F.</p>
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<p>Free particle distribution characteristics curve. (<b>a</b>) Group A; (<b>b</b>) Group B; (<b>c</b>) Group C; (<b>d</b>) Group D; (<b>e</b>) Group E; (<b>f</b>) Group F.</p>
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<p>Depth–free particle proportion relationship. (<b>a</b>) Group A; (<b>b</b>) Group B; (<b>c</b>) Group C; (<b>d</b>) Group D; (<b>e</b>) Group E; (<b>f</b>) Group F.</p>
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<p>Effective seepage pore distribution characteristics curve. (<b>a</b>) Group A; (<b>b</b>) Group B; (<b>c</b>) Group C; (<b>d</b>) Group D; (<b>e</b>) Group E; (<b>f</b>) Group F.</p>
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<p>Depth–effective seepage pore proportion relationship. (<b>a</b>) Group A; (<b>b</b>) Group B; (<b>c</b>) Group C; (<b>d</b>) Group D; (<b>e</b>) Group E; (<b>f</b>) Group F.</p>
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<p>Relationships of (<b>a</b>) free particle proportion–permeability and (<b>b</b>) effective seepage pore proportion–permeability.</p>
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<p>(<b>a</b>–<b>f</b>) Particle distribution difference curve of Group A−F; (<b>g</b>–<b>l</b>) cumulative particle distribution difference curve of Group A−F.</p>
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<p>(<b>a</b>–<b>f</b>) Pore distribution difference curve of Group A−F; (<b>g</b>–<b>l</b>) cumulative pore distribution difference curve of Group A−F.</p>
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<p>Depth–effective seepage pore proportion/total porosity relationship. (<b>a</b>) Group A; (<b>b</b>) Group B; (<b>c</b>) Group C; (<b>d</b>) Group D; (<b>e</b>) Group E; (<b>f</b>) Group F.</p>
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<p>Permeability of various rock samples at different depths.</p>
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<p>B3–A3 and F2–A2 (<b>a</b>) particle difference distribution curve, (<b>b</b>) cumulative particle difference curve, (<b>c</b>) pore difference distribution curve, (<b>d</b>) cumulative pore difference curve.</p>
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10 pages, 1313 KiB  
Systematic Review
Systematic Review and Meta-Analysis of Intervention Techniques in Occupational Therapy for Babies and Children with Obstetric Brachial Plexus Palsy
by María Martínez-Carlón-Reina, Janine Hareau-Bonomi, Mª Pilar Rodríguez-Pérez and Elisabet Huertas-Hoyas
J. Clin. Med. 2024, 13(20), 6186; https://doi.org/10.3390/jcm13206186 (registering DOI) - 17 Oct 2024
Abstract
(1) Background: Obstetric brachial plexus palsy (OBPP) is an unpredictable and unpreventable neurological injury, caused by shoulder dystocia during birth, that affects the brachial plexus and leads to motor and sensory deficits in the child’s upper extremity. The limited literature on early [...] Read more.
(1) Background: Obstetric brachial plexus palsy (OBPP) is an unpredictable and unpreventable neurological injury, caused by shoulder dystocia during birth, that affects the brachial plexus and leads to motor and sensory deficits in the child’s upper extremity. The limited literature on early therapeutic assessment of newborns with OBPP highlights a gap in specialized care that, if filled, could enhance decision-making and support timely treatment. The objective of this paper is to analyze the therapeutic intervention techniques used at an early stage and their functional impact, from the occupational therapy discipline in the treatment of the upper extremity in babies and children with OBPP. (2) Method: Systematic review design and meta-analysis. A systematic review is a comprehensive analysis of existing research on a specific topic, using rigorous methods to identify, evaluate, and synthesize studies. Meta-analysis, often part of a systematic review, combines results from multiple studies to identify overall trends and enhance reliability, providing a clearer summary of evidence. Articles that included pediatric patients (from birth to 12 years of age) with a diagnosis of OBPP were reviewed. The results of the techniques used were analyzed according to each study, with the scale or method of assessment considered by the study for the presentation of data. The articles were assessed for methodological quality using the “PEDro Validity Scale”. (3) Results: A total of 2190 articles were found, with 108 analyzed and 22 fully meeting this study’s standards. Fourteen had a quantitative design, while the others included clinical guidelines. The most statistically reliable intervention techniques were CIMT (constraint-induced movement therapy) and splinting (dynamic and static), with second-tier techniques like joint manipulation, NMES, early infant management education, and serial casting used when needed. This study focused on children from birth to eight years old, with assessment tools primarily measuring upper limb range of motion, external rotation, supination, and impairment levels, though bimanual activity assessment was less common. (4) Conclusions: The early implementation of the techniques that provide us with the most data are CIMT, splinting, NMES, and joint manipulation linked to health education for families. In second place, we have the use of TB infiltrations and serial casts, when the treatment of the previous techniques fails in some cases. Full article
(This article belongs to the Section Clinical Neurology)
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<p>Flux plot: flux diagram for systematic review and meta-analysis.</p>
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<p>Improvement graph within scale range.</p>
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<p>Forest plot [<a href="#B18-jcm-13-06186" class="html-bibr">18</a>,<a href="#B19-jcm-13-06186" class="html-bibr">19</a>].</p>
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15 pages, 70999 KiB  
Article
Lightweight Infrared Image Denoising Method Based on Adversarial Transfer Learning
by Wen Guo, Yugang Fan and Guanghui Zhang
Sensors 2024, 24(20), 6677; https://doi.org/10.3390/s24206677 (registering DOI) - 17 Oct 2024
Abstract
A lightweight infrared image denoising method based on adversarial transfer learning is proposed. The method adopts a generative adversarial network (GAN) framework and optimizes the model through a phased transfer learning strategy. In the initial stage, the generator is pre-trained using a large-scale [...] Read more.
A lightweight infrared image denoising method based on adversarial transfer learning is proposed. The method adopts a generative adversarial network (GAN) framework and optimizes the model through a phased transfer learning strategy. In the initial stage, the generator is pre-trained using a large-scale grayscale visible light image dataset. Subsequently, the generator is fine-tuned on an infrared image dataset using feature transfer techniques. This phased transfer strategy helps address the problem of insufficient sample quantity and variety in infrared images. Through the adversarial process of the GAN, the generator is continuously optimized to enhance its feature extraction capabilities in environments with limited data. Moreover, the generator structure incorporates structural reparameterization technology, edge convolution modules, and progressive multi-scale attention block (PMAB), significantly improving the model’s ability to recognize edge and texture features. During the inference stage, structural reparameterization further optimizes the network architecture, significantly reducing model parameters and complexity and thereby improving denoising efficiency. The experimental results of public and real-world datasets demonstrate that this method effectively removes additive white Gaussian noise from infrared images, showing outstanding denoising performance. Full article
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<p>Overall network architecture.</p>
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<p>Schematic diagram of the generator.</p>
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<p>Structurally reparameterizable edge convolution block.</p>
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<p>The structure of progressive multi-scale attention block (PMAB).</p>
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<p>The structure of convolutional block attention module (CBAM).</p>
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<p>The structure of discriminator.</p>
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<p>Schematic diagram of model parameter transfer.</p>
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<p>Denoising results of different methods on the AWGN with a variance of σ = 15.</p>
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<p>Denoising results of different methods on the AWGN with a variance of σ = 25.</p>
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<p>Denoising results of different methods on the AWGN with a variance of σ = 50.</p>
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<p>Real indoor infrared image (water dispenser).</p>
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<p>Infrared image of an outdoor scene (tree trunk).</p>
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<p>Comparison graph of the loss function.</p>
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11 pages, 732 KiB  
Article
Assessing Influence of Mismatch Repair Mutations on Survival in Patients After Resection of Pancreatic Ductal and Periampullary Adenocarcinoma
by Elizabeth Prezioso, Eleanor Mancheski, Kylee Shivok, Zachary Kaplan, Wilbur Bowne, Aditi Jain, Harish Lavu, Charles J. Yeo and Avinoam Nevler
J. Clin. Med. 2024, 13(20), 6185; https://doi.org/10.3390/jcm13206185 (registering DOI) - 17 Oct 2024
Abstract
Background: Pancreatic ductal adenocarcinoma (PDAC) is the third leading cause of cancer-related deaths in the United States. Previous studies have indicated that microsatellite instability and deficient mismatch repair (MMR) may be associated with improved survival in patients with pancreatic cancer. Here, we [...] Read more.
Background: Pancreatic ductal adenocarcinoma (PDAC) is the third leading cause of cancer-related deaths in the United States. Previous studies have indicated that microsatellite instability and deficient mismatch repair (MMR) may be associated with improved survival in patients with pancreatic cancer. Here, we aim to investigate the impact of deficient MMR (dMMR) status on oncologic outcomes in patients after resection of PDAC and periampullary adenocarcinoma. Methods: This is a single-institution, retrospective study based on a prospectively maintained database. Pancreatic ductal adenocarcinoma (N = 342) and periampullary adenocarcinoma patients (N = 76) who underwent pancreatic resection surgery between 2016 and 2021 were included. Immunohistochemistry staining results of MMR proteins and next-generation sequencing data were recorded. Cancer-type dependent Cox regression analyses were performed to assess overall and disease-free survival, which was complemented with a 1:2 propensity-score matching for each of the cancer types in order to compare oncologic outcomes. Results: A total of 418 pancreatic cancer patients were included in the analysis. Fifteen patients (3.5%) were diagnosed as dMMR (PDAC N = 7 and periampullary adenocarcinoma N = 8). Cox regression modeling of dMMR status interaction with TNM staging and cancer type revealed that dMMR status strongly improves overall survival (p < 0.05). After propensity-score matching, Cox regression identified dMMR status as a significant marker of improved overall survival (HR = 0.27, 95%CI 0.09–0.88, p = 0.029). Conclusions: Overall, our findings suggest that dMMR status is associated with markedly improved survival outcomes in patients after resection of pancreatic and periampullary cancer. Future large-scale studies are needed to further validate this finding. Full article
(This article belongs to the Special Issue Targeted Treatment of Pancreatic Cancer)
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<p>Cox regression models for (<b>A</b>) overall survival and (<b>B</b>) disease-free survival (N = 418). dMMR—deficient MMR; pMMR—proficient MMR.</p>
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<p>Propensity-score-matched (1:2) and weighted Cox regression model for overall survival analysis in pancreatic ductal adenocarcinoma (<b>A</b>) and periampullary cancer patients (<b>B</b>), assessing the impact of deficient MMR on the two cancer types (dMMR = 15/pMMR = 30).</p>
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10 pages, 246 KiB  
Article
Seed Germination Responses to Temperature and Osmotic Stress Conditions in Brachiaria Forage Grasses
by Francuois L. Müller, Jabulile E. Leroko, Clement F. Cupido, Igshaan Samuels, Nothando Ngcobo, Elizabeth L. Masemola, Fortune Manganyi-Valoyi and Tlou Julius Tjelele
Grasses 2024, 3(4), 264-273; https://doi.org/10.3390/grasses3040019 (registering DOI) - 17 Oct 2024
Abstract
Brachiaria forages are known to be drought-tolerant as mature plants, but no information about drought tolerance at the seed germination stage is currently available. This study aimed to determine the impacts of different temperature and moisture conditions on the seed germination characteristics of [...] Read more.
Brachiaria forages are known to be drought-tolerant as mature plants, but no information about drought tolerance at the seed germination stage is currently available. This study aimed to determine the impacts of different temperature and moisture conditions on the seed germination characteristics of five Brachiaria genotypes. Brachiaria seeds were germinated under constant temperatures of 5 °C–45 °C at increments of 5 °C. Within each temperature treatment, five osmotic treatments (0 MPa, −0.1 MPa, −0.3 MPa, −0.5 MPa, and −0.7 MPa) were applied, and germination was recorded daily for 20 days. The results showed that seed germination in all Brachiaria species was significantly negatively impacted (p < 0.05) by osmotic stress as well as by high and low temperatures. For all species, germination only occurred between 15 and 40 °C. Under optimum moisture conditions (0 MPa), the optimum germination temperatures for B. humidicola were 15 to 35 °C, for B. brizantha and B. nigropedata, they were 15 to 20 °C, for B. decumbens, they were 15 to 25 °C, and for the hybrid Brachiaria species, the optimum germination temperature was only 20 °C. In all species, seed germination decreased as moisture conditions became more limiting. Only B. humidicola germinated optimally at a high temperature (35 °C). At these temperatures, the species had more than 82% germination when moisture was not a limiting factor (0 MPa), but at low osmotic stress conditions (−0.1 MPa) at 30 °C, the germination of this species decreased to 67%. In conclusion, the results from this study indicate that the seed germination and early seedling establishment stages of Brachiaria grasses are only moderately tolerant to drought stress. Further work on early seedling responses to temperature and moisture stresses is needed to quantify early seedling responses to these stresses and to develop more detailed planting time guidelines for farmers. Full article
24 pages, 5552 KiB  
Article
Improving Simulations of Rice Growth and Nitrogen Dynamics by Assimilating Multivariable Observations into ORYZA2000 Model
by Jinmin Li, Liangsheng Shi, Jingye Han, Xiaolong Hu, Chenye Su and Shenji Li
Agronomy 2024, 14(10), 2402; https://doi.org/10.3390/agronomy14102402 (registering DOI) - 17 Oct 2024
Abstract
The prediction of crop growth and nitrogen status is essential for agricultural development and food security under climate change scenarios. Crop models are powerful tools for simulating crop growth and their responses to environmental variables, but accurately capturing the dynamic changes in crop [...] Read more.
The prediction of crop growth and nitrogen status is essential for agricultural development and food security under climate change scenarios. Crop models are powerful tools for simulating crop growth and their responses to environmental variables, but accurately capturing the dynamic changes in crop nitrogen remains a considerable challenge. Data assimilation can reduce uncertainties in crop models by integrating observations with model simulations. However, current data assimilation research is primarily focused on a limited number of observational variables, and insufficiently utilizes nitrogen observations. To address these challenges, this study developed a new multivariable data assimilation system, ORYZA-EnKF, that is capable of simultaneously integrating multivariable observations (including development stage, DVS; leaf area index, LAI; total aboveground dry matter, WAGT; and leaf nitrogen concentration, LNC). Then, the system was tested through three consecutive years of field experiments from 2021 to 2023. The results revealed that the ORYZA-EnKF model significantly improved the simulations of crop growth compared to the ORYZA2000 model. The relative root mean squared error (RRMSE) for LAI simulations decreased from 23–101% to 16–47% in the three-year experiment. Moreover, the incorporation of LNC observations enabled more accurate predictions of rice nitrogen dynamics, with RRMSE for LNC simulations reduced from 16–31% to 14–26%. And, the RRMSE decreased from 32–50% to 30–41% in the simulations of LNC under low-nitrogen conditions. The multivariable data assimilation system demonstrated its effectiveness in improving crop growth simulations and nitrogen status predictions, providing valuable insights for precision agriculture. Full article
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<p>Flowchart of the construction of the ORYZA-EnKF data assimilation system.</p>
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<p>(<b>a</b>) Location of Hengsha township, Shanghai city in China; (<b>b</b>) Location of two experiment sites (Yongfa village and Fumin village) in Hengsha Township; (<b>c</b>) Overview of 12 experimental plots in Yongfa village; (<b>d</b>) Overview of 24 experimental plots in Fumin village.</p>
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<p>Sensitivity indices of parameters in the ORYZA2000 model for rice yield under different nitrogen application scenarios: (<b>a</b>) the first-order sensitivity index and (<b>b</b>) the total sensitivity index. N0, N200, N300, and N400 refer to the total N rate of 0, 200, 300, and 400 kg N ha<sup>−1</sup>, respectively.</p>
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<p>Simulation results of rice state variables for three years by ORYZA2000 model. (<b>a1</b>–<b>a3</b>) DVS simulation results for 2021, 2022, and 2023; (<b>b1</b>–<b>b3</b>) LAI simulation results for 2021, 2022, and 2023; (<b>c1</b>–<b>c3</b>) WAGT simulation results for 2021, 2022, and 2023; (<b>d1</b>–<b>d3</b>) LNC simulation results for 2021, 2022, and 2023; and (<b>e1</b>–<b>e3</b>) Yield simulation results for 2021, 2022, and 2023.</p>
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<p>Influence of different observed variables on the ORYZA-EnKF data assimilation system. (<b>a1</b>–<b>e1</b>) Simulation results for DVS, LAI, WAGT, LNC, and yield when only DVS observations were assimilated; (<b>a2</b>–<b>e2</b>) Results when only LAI observations were assimilated; (<b>a3</b>–<b>e3</b>) Results when only WAGT observations were assimilated; (<b>a4</b>–<b>e4</b>) Results when only LNC observations were assimilated; and (<b>a5</b>–<b>e5</b>) Results when all observations were assimilated. The different columns represent the observations assimilated by ORYZA-EnKF, and the different rows are the simulation results of the model state variables. The black dots and lines refer to OSS observations; the red lines are open-loop simulation results; the thin lines in light blue are the simulation results of the different samples, and the thick lines in blue are the averages of the samples.</p>
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<p>Effects of nitrogen observation on the ORYZA-EnKF data assimilation system. (<b>a</b>–<b>e</b>) represent the simulation results for DVS, LAI, WAGT, LNC, and yield, respectively. The black dots and lines refer to OSS observations; the red lines are open-loop simulation results; the green line in Case 1 is the simulation result of all observed variables, and the blue line in Case 6 represents the result of removing the observation of leaf nitrogen content from all observed variables.</p>
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<p>Simulation of rice LNC by the ORYZA-EnKF data assimilation system. The legend is labeled with year + test field location + nitrogen application level. (<b>a1</b>–<b>a3</b>) 2021-YF; (<b>b1</b>–<b>b3</b>) 2022-YF; (<b>c1</b>–<b>c3</b>) 2023-FM.</p>
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<p>Simulation of the rice LAI by the ORYZA-EnKF data assimilation system. The legend is labeled with year + test field location + nitrogen application level. (<b>a1</b>,<b>a2</b>) 2021-YF; (<b>b1</b>,<b>b2</b>) 2022-YF; (<b>c1</b>,<b>c2</b>) 2023-FM.</p>
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<p>Simulation of WAGT by the ORYZA-EnKF data assimilation system. The legend is labeled with year + test field location + nitrogen application level. (<b>a1</b>,<b>a2</b>) 2021-YF; (<b>b1</b>,<b>b2</b>) 2022-YF; (<b>c1</b>,<b>c2</b>) 2023-FM.</p>
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42 pages, 113259 KiB  
Article
Hypogene Alteration of Base–Metal Mineralization at the Václav Vein (Březové Hory Deposit, Příbram, Czech Republic): The Result of Recurrent Infiltration of Oxidized Fluids
by Zdeněk Dolníček, Jiří Sejkora and Pavel Škácha
Minerals 2024, 14(10), 1038; https://doi.org/10.3390/min14101038 (registering DOI) - 17 Oct 2024
Abstract
The Václav vein (Březové Hory deposit, Příbram ore area, Czech Republic) is a base–metal vein containing minor Cu-Zn-Pb-Ag-Sb sulfidic mineralization in a usually hematitized gangue. A detailed mineralogical study using an electron microprobe revealed a complicated multistage evolution of the vein. Early siderite [...] Read more.
The Václav vein (Březové Hory deposit, Příbram ore area, Czech Republic) is a base–metal vein containing minor Cu-Zn-Pb-Ag-Sb sulfidic mineralization in a usually hematitized gangue. A detailed mineralogical study using an electron microprobe revealed a complicated multistage evolution of the vein. Early siderite and Fe-rich dolomite were strongly replaced by assemblages of hematite+rhodochrosite and hematite+kutnohorite/Mn-rich dolomite, respectively. In addition, siderite also experienced strong silicification. These changes were associated with the dissolution of associated sulfides (sphalerite, galena). The following portion of the vein contains low-Mn dolomite and calcite gangue with Zn-rich chlorite, wittichenite, tetrahedrite-group minerals, chalcopyrite, bornite, and djurleite, again showing common replacement textures in case of sulfides. The latest stage was characterized by the input of Ag and Hg, giving rise to Ag-Cu sulfides, native silver (partly Hg-rich), balkanite, and (meta)cinnabar. We explain the formation of hematite-bearing oxidized assemblages at the expense of pre-existing “normal” Příbram mineralization due to repeated episodic infiltration of oxygenated surface waters during the vein evolution. Episodic mixing of ore fluids with surface waters was suggested from previous stable isotope and fluid inclusion studies in the Příbram ore area. Our mineralogical study thus strengthens this genetic scenario, illustrates the dynamics of fluid movement during the evolution of a distinct ore vein structure, and shows that the low content of ore minerals cannot be necessarily a primary feature of a vein. Full article
(This article belongs to the Special Issue Mineralogy and Geochemistry of Polymetallic Ore Deposits)
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<p>The youngest mineral assemblage of a drusy cavity in sample P1N 9430. (<b>a</b>) A top view across the whole drusy cavity. (<b>b</b>) Calcite crystals with acicular ore aggregates. (<b>c</b>) Smooth and finely wrinkled surface of acicular ore aggregates. (<b>d</b>) Acicular ore aggregates enclosed in a transparent crystal of calcite.</p>
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<p>Geological position of the Březové Hory deposit in the Příbram ore area (modified from [<a href="#B1-minerals-14-01038" class="html-bibr">1</a>]). BHD—Březové Hory base–metal district, PUD—Příbram uranium district. Positions of some other sites mentioned in the text are also indicated.</p>
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<p>Position of the Václav vein in geological cross-section through the Březové Hory ore district (modified from [<a href="#B9-minerals-14-01038" class="html-bibr">9</a>]). The Anna shaft is situated north of the Prokop shaft, out of the section line.</p>
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<p>The macroscopic appearance of the sample P1N 9430 with marked zones A–E and sites, from which samples for preparation of polished sections were cut off. The left part of the figure illustrates the distribution of selected mineral phases. Sample width is 4.5 cm.</p>
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<p>Mineral assemblage and textures of the Václav vein in the BSE images. (<b>a</b>) A slightly zoned relic of siderite replaced by surrounding quartz, carbonates of the dolomite-kutnohorite series, hematite, and tetrahedrite. Right part is wall rock. Zone A, sample VA-1. (<b>b</b>) Relic of siderite strongly replaced by hematite, rhodochrosite, and zoned carbonates of the dolomite series. Zone A, sample VA-2. (<b>c</b>) Relic of siderite rimmed by rhodochrosite and hematite. Zone A, sample VA-2. (<b>d</b>) Euhedral rhodochrosite crystal enclosed in hematite in the proximity of a relic of siderite, which is strongly replaced by the rhodochrosite rim and carbonates of the dolomite group. Zone A, sample VA-2. (<b>e</b>) Boundary between Zone A (hematite-rich on the left) and Zone B (hematite-poor on the right) separated by quartz crystals. Replacement of Do-I by Do-II is observed in the right part. Sample VA-5. (<b>f</b>) Detailed view on replacement of Do-I by zoned Do-II containing inclusions of hematite. Zone B, sample VA-5.</p>
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<p>Variations in the chemical composition of siderite, rhodochrosite, and calcite from the Václav vein in comparison with published data. (<b>a</b>) Siderite and rhodochrosite in the classification diagram by [<a href="#B27-minerals-14-01038" class="html-bibr">27</a>]. (<b>b</b>) Fe vs. Mn and Fe vs. Mg plots for calcite. Comparative data for other deposits of the Příbram uranium and base metal district are from [<a href="#B5-minerals-14-01038" class="html-bibr">5</a>,<a href="#B6-minerals-14-01038" class="html-bibr">6</a>,<a href="#B7-minerals-14-01038" class="html-bibr">7</a>,<a href="#B28-minerals-14-01038" class="html-bibr">28</a>].</p>
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<p>Variations in the chemical composition of carbonates of the dolomite-ankerite series from the Václav vein in comparison with published data. (<b>a</b>) All data in the classification diagram by [<a href="#B27-minerals-14-01038" class="html-bibr">27</a>]. (<b>b</b>) Data sorted according to Zones. (<b>c</b>) Data arbitrarily grouped according to compositional similarities. Comparative data for other deposits of the Příbram uranium and base metal district are from [<a href="#B5-minerals-14-01038" class="html-bibr">5</a>,<a href="#B6-minerals-14-01038" class="html-bibr">6</a>,<a href="#B7-minerals-14-01038" class="html-bibr">7</a>,<a href="#B28-minerals-14-01038" class="html-bibr">28</a>]. PUD–average dolomite from the Příbram uranium and base–metal district according to wet-chemical analyses by [<a href="#B29-minerals-14-01038" class="html-bibr">29</a>].</p>
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<p>Mineral assemblage and textures of the Václav vein. (<b>a</b>) Contact between Do-II and Do-III dolomites. Note the corrosion of Do-II by Do-III just along the contact. Zone C, sample VA-7. (<b>b</b>) Zoned crystals of carbonates of the dolomite group (bright Do-II is overgrown by darker Do-III) growing over lenticular hematite crystals. Residual vug was later filled up by calcite with aggregates of tetrahedrite and chalcopyrite. Zone A, black domain, sample VA-6. (<b>c</b>) The latest Do-III dolomite crystals overgrown by chalcopyrite-bornite aggregates and calcite. Zone D, sample VA-9. (<b>d</b>) Aggregates of Zn-rich chlorite filling together with calcite residual cavities in the vein composed of euhedral lenticular hematite crystals, sulfidic aggregates, and Do-II+Do-III carbonates. Zone A, black domain, sample VA-6. (<b>e</b>) Two generations of hematite strongly differing in the quality of the polished surface. Fine-grained early hemispherical aggregates are poorly polished, whereas the latest hematite preceding crystallization of Do-II carbonate followed by sulfides is well polished. Sulfide aggregate is composed of sphalerite, chalcopyrite, tetrahedrite-(Zn) (Ttd-I), and an unknown reddish AgCu<sub>6</sub>Fe<sub>2</sub>S<sub>8</sub> phase. Zone A, sample VA-1. (<b>f</b>) The central area of Figure (<b>e</b>) in BSE image. Note the zonality of hematite and Do-II carbonate. Zone A, sample VA-1. Figure (<b>e</b>) is taken in plane-polarized reflected light, whereas the other pictures are BSE images.</p>
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<p>Variations in the chemical composition of chlorite from the Václav vein and comparison with published data. (<b>a</b>) A Fe-Mg-Zn plot. (<b>b</b>) The Ca vs. Si plot. The comparative data from the Jerusalem deposit (Příbram uranium district) are from [<a href="#B5-minerals-14-01038" class="html-bibr">5</a>].</p>
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<p>Mineral assemblage and textures of the Václav vein. (<b>a</b>) Concentric zonation of hematite. Zone A, sample VA-2. (<b>b</b>) Patchy zonation of hematite caused by variable Sb contents. The youngest part of Zone A, sample VA-3. (<b>c</b>) The strongly corroded cassiterite hosted by sphalerite (partly Cu,Sn-enriched) replaced by hematite+Do-II aggregate. Zone A, sample VA-4. (<b>d</b>) Finely porous and non-porous bornite and chalcopyrite. Note a bluish tint of a part of porous bornite. Sample Dy-817. (<b>e</b>) Finely porous and non-porous bornite and chalcopyrite, with a short veinlet of tetrahedrite. Note the compositional homogeneity of bornite. Sample Dy-817. (<b>f</b>) An acicular polymineral aggregate composed of bornite, covellite, and Ag-Cu sulfides (Ag-Cu-S) with thick symmetrical rims of chalcopyrite I (Cpy-I). Zone E, sample Dy-973. Figures (<b>d</b>,<b>f</b>) are taken in plane-polarized reflected light, whereas the other pictures are BSE images.</p>
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<p>Variations in the chemical composition of hematite from the Václav vein. (<b>a</b>) The Si vs. Al plot. (<b>b</b>) The Si vs. Sb plot. (<b>c</b>) The Me<sup>2+</sup> vs. Sb plot. (<b>d</b>) The Pb vs. Sb plot.</p>
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<p>Mineral assemblage and textures of the Václav vein. (<b>a</b>) A crust formed by chalcopyrite (Cpy-I), partly filled and enclosed by bornite and overgrown by a tetrahedrite (Ttd-III) crystal. Part of the pores in bornite was filled by covellite and Ag-Cu sulfides. Bornite contains ribbons of chalcopyrite (Cpy-II). Zone E, sample Dy-973. (<b>b</b>) Three morphological forms of chalcopyrite, crust (Cpy-I), ribbon (Cpy-II), and symplectite with mckinstryite (Symplectite), hosted by bornite with late rims and fillings of covellite and Ag-Cu(-Hg)-S phases. The black rectangle shows the area of <a href="#minerals-14-01038-f012" class="html-fig">Figure 12</a>c. Zone E, sample Dy-973. (<b>c</b>) BSE detail of the central part of Figure (c) showing the nature of Ag-Cu(-Hg)-S phases: fine intergrowths of stromeyerite and mckinstryite are partly overgrown by balkanite. (<b>d</b>) Sphalerite grains are rimmed by bornite and chalcopyrite. Note the intense corrosion of both earlier sulfide phases by later ones. Zone A, sample VA-1. (<b>e</b>) Sphalerite rimmed by bornite, tetrahedrite (Ttd-I), and two generations of chalcopyrite differing in porosity. Note early porous chalcopyrite Cpy-I is replaced by Ttd-I. Zone D, sample VA-9. (<b>f</b>) Bright Ag-enriched zone in chalcopyrite. Zone D, sample VA-8. Figures (<b>c</b>,<b>f</b>) are BSE images; the other pictures are taken in plane-polarized reflected light.</p>
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<p>Variations in the chemical composition of some ore minerals from the Václav vein in comparison with published data. (<b>a</b>) Graph Ag versus Sb for chalcopyrite. (<b>b</b>) Graph Ag versus Cu for bornite. (<b>c</b>) Graph Sn versus Cu for sphalerite. (<b>d</b>) Graph Fe versus Cd for sphalerite. (<b>e</b>) Graph Ag versus Cu+Fe+Cd for mckinstryite. (<b>f</b>) Graph Ag versus Cu for balkanite. Comparative data for sphalerite from the Háje deposit (Příbram uranium district) are from [<a href="#B7-minerals-14-01038" class="html-bibr">7</a>], for mckinstryite from Milín from [<a href="#B31-minerals-14-01038" class="html-bibr">31</a>], for other mckinstryite data from [<a href="#B32-minerals-14-01038" class="html-bibr">32</a>], for danielsite from [<a href="#B33-minerals-14-01038" class="html-bibr">33</a>], and for published balkanite data from [<a href="#B5-minerals-14-01038" class="html-bibr">5</a>,<a href="#B34-minerals-14-01038" class="html-bibr">34</a>,<a href="#B35-minerals-14-01038" class="html-bibr">35</a>,<a href="#B36-minerals-14-01038" class="html-bibr">36</a>,<a href="#B37-minerals-14-01038" class="html-bibr">37</a>].</p>
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<p>Mineral assemblage and textures of the Václav vein. (<b>a</b>) Irregular aggregate of djurleite partly replaced by bornite, which is rimmed by a non-continuous zone of chalcopyrite and small grains of Ag-Cu-Hg-S phases. Note the abundant ribbons of chalcopyrite in the outer part of bornite adjacent to the chalcopyrite rim. Sample Dy-816. (<b>b</b>) Sphalerite rimmed by bornite (with brighter Ag-enriched domains) and then by chalcopyrite. Late microfractures contain Ag-Cu-S phases. Zone D, sample VA-9. (<b>c</b>) The brighter Cu,Sn-enriched domains in sphalerite in the vicinity of strongly corroded grains of cassiterite. Zone A, sample VA-4. (<b>d</b>) Sphalerite with brighter Cd-enriched domains (in the lower part of the grain), rimmed by chalcopyrite and enclosing grains of wittichenite and Bi-enriched tetrahedrite. Zone A, sample VA-4. Inset–Oscillatory zoning of a sphalerite grain due to changing Cd contents. Zone C, sample VA-7. (<b>e</b>) Mn-enriched sphalerite and hematite in the residual cavity in Mn-rich dolomite Do-II replacing Fe-rich dolomite Do-I. Zone B, sample VA-5. (<b>f</b>) Tetrahedrite Ttd-I is cut by veinlets of bornite and chalcopyrite Cpy-II. Zone D, sample VA-9. Figures (<b>a</b>,<b>f</b>) are taken in plane-polarized reflected light; the other pictures are BSE images.</p>
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<p>Mineral assemblage and textures of the Václav vein in BSE images. (<b>a</b>) Zonality of a tetrahedrite Ttd-I aggregate is largely caused by Fe-Zn substitution and also, exceptionally, by high Cd (arrowed). The Ttd-I is cut by veinlets of Bi-bearing Ttd-II and a narrow overgrowth of Ttd-III is observed in the lower part of the photograph. Zone D, sample VA-9. (<b>b</b>) Oscillatory zoned Bi-bearing Ttd-II rimming a grain of chalcopyrite. Zone A, sample VA-6. (<b>c</b>) Patchy zoning of Ttd-II. The brightest domain already corresponds to annivite-(Zn). Zone A, sample VA-6. (<b>d</b>) Zoned Bi-bearing tetrahedrite Ttd-II rimming and cutting tetrahedrite Ttd-I. The brightest domain corresponds to <span class="html-italic">annivite-</span>(<span class="html-italic">Cu</span>). Surrounding sphalerite encloses wittichenite. Zone A, sample VA-3. (<b>e</b>) Nature of Ag-Cu sulfides in chalcopyrite-hosted “acicular” polymineral aggregate from <a href="#minerals-14-01038-f010" class="html-fig">Figure 10</a>f: small domains of jalpaite are hosted by the mckinstryite matrix. Zone E, sample Dy-973. (<b>f</b>) A finely porous aggregate of Hg-absent native silver partly rimmed by stromeyerite. Zone E, sample Dy-973.</p>
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<p>Variations in the chemical composition of tetrahedrite-group minerals from the Václav vein (data points) in comparison with published data (outlined). (<b>a</b>) Graph Fe-Zn-Cd. (<b>b</b>) Graph As-Sb-Bi. Data in at. %. Comparative data for Jáchymov and Hřebečná sites are from [<a href="#B41-minerals-14-01038" class="html-bibr">41</a>]. Notably, data from the Příbram ore area are not visualized as they exhibit Fe-Zn and Sb-As substitutions only.</p>
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<p>Mineral assemblage and textures of the Václav vein in BSE images. (<b>a</b>) Balkanite-like phase rimming grains of probable (meta)cinnabar. Zone A, sample VA-1. (<b>b</b>) Grains of unspecified Ag-Cu-Hg-S phases (white) with variable compositions growing on a chalcopyrite finger-like aggregate. Zone E, sample Dy-973. (<b>c</b>) Individual grains of likely galena (GA) and unspecified Ag-Cu-Hg-S phases with variable compositions growing on a djurleite-bornite-chalcopyrite aggregate. Sample Dy-816. (<b>d</b>) An unknown (Cu,Ag)<sub>4</sub>FeS<sub>4</sub> phase associated with covellite in a sphalerite-chalcopyrite-hematite aggregate. Zone A, sample VA-3.</p>
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<p>Variations in the chemical composition of some ore minerals from the Václav vein in comparison with published data. (<b>a</b>) Graph Cu/Ag versus Hg for balkanite. (<b>b</b>) Graph Ag versus Cu or Hg for balkanite and possible intergrowths of Ag-Cu-Hg phases. (<b>c</b>) Graph Hg versus Cu for balkanite and possible intergrowths of Ag-Cu-Hg phases. (<b>d</b>) Graph Hg versus Ag+Cu+Fe for balkanite and possible intergrowths of Ag-Cu-Hg phases. Comparative published balkanite data are from [<a href="#B5-minerals-14-01038" class="html-bibr">5</a>,<a href="#B34-minerals-14-01038" class="html-bibr">34</a>,<a href="#B35-minerals-14-01038" class="html-bibr">35</a>,<a href="#B36-minerals-14-01038" class="html-bibr">36</a>,<a href="#B37-minerals-14-01038" class="html-bibr">37</a>], and data for danielsite are from [<a href="#B33-minerals-14-01038" class="html-bibr">33</a>].</p>
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<p>A sketch showing the interpreted textural evolution of the late ore assemblage from a drusy cavity of the sample P1N 9430. The crystallization of an acicular mineral (<b>a</b>) was followed by the deposition of a continuous layer of early chalcopyrite Cpy-I on its crystals (<b>b</b>). Then, the dissolution of acicular mineral took place (<b>c</b>), followed by the crystallization of bornite inside and outside of Cpy-I crusts (<b>d</b>). The crystallization of late chalcopyrite Cpy-II and tetrahedrite Ttd-III (<b>e</b>) was followed by minor fracturing of early ores and partial healing of the residual porosity by the latest ore minerals including covellite and Ag-Cu(-Hg) sulfides (<b>f</b>).</p>
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<p>The simplified paragenetic scheme of the Václav vein. Note that the position of some mineral phases is questionable (marked by ?); more problematic phases are missing.</p>
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<p>The interpreted scenario of the origin of the studied mineralization from the Václav vein. (<b>a</b>) Early stage characterized by the escape of “deep” fluids. (<b>b</b>) Late stage involving the circulation of basinal fluids—Scenario I. (<b>c</b>) Late stage involving the circulation of basinal fluids–Scenario II. Arrows characterize the direction of fluid movement.</p>
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8 pages, 619 KiB  
Article
Perioperative and Long-Term Outcomes After Combined Liver and Kidney Transplantation: A Single-Center Experience
by Kosta Cerović, Benjamin Hadžialjević, Simon Hawlina and Blaž Trotovšek
Life 2024, 14(10), 1319; https://doi.org/10.3390/life14101319 (registering DOI) - 17 Oct 2024
Abstract
Combined liver–kidney transplantation (CLKT) has evolved as a therapeutic option for patients with concurrent end-stage liver and renal diseases. This study evaluates the perioperative and long-term outcomes of CLKT at a single center in Slovenia, highlighting the challenges and successes of simultaneous organ [...] Read more.
Combined liver–kidney transplantation (CLKT) has evolved as a therapeutic option for patients with concurrent end-stage liver and renal diseases. This study evaluates the perioperative and long-term outcomes of CLKT at a single center in Slovenia, highlighting the challenges and successes of simultaneous organ transplantation. We retrospectively analyzed all patients undergoing simultaneous CLKT at the University Medical Centre Ljubljana from April 2014 to June 2023. Data on demographics, cause of liver and kidney disease, operative details, postoperative complications, patient and graft survival, and follow-up were collected and analyzed. Five patients aged 27 to 60 years underwent CLKT within the study period. All transplants involved deceased donors with whole-liver grafts. Indications for CLKT were polycystic liver disease (n = 3), Caroli’s disease (n = 1), and alcoholic cirrhosis (n = 1). The mean follow-up duration was 45.2 months, with a 100% survival rate. The incidence of surgical and postoperative complications was low. This pioneering series of simultaneous CLKTs in Slovenia demonstrates the feasibility and effectiveness of the procedure in smaller transplant centers. Despite challenges, including T cell-mediated kidney rejection and surgical complications, the study emphasizes the importance of comprehensive postoperative care and management in optimizing outcomes for CLKT recipients. Full article
(This article belongs to the Section Medical Research)
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<p>A contrast-enhanced abdominal computed tomography (CT) scan shows a hypodense area in the lower parts of segments 5 and 6 of the transplanted liver, resulting from an obstruction of flow to a branch of the right hepatic artery (white arrow). In addition, the transplanted kidney can be seen in the right iliac fossa (white arrowhead).</p>
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42 pages, 1388 KiB  
Review
Fault Diagnosis and Prognosis of Satellites and Unmanned Aerial Vehicles: A Review
by MohammadSaleh Hedayati, Ailin Barzegar and Afshin Rahimi
Appl. Sci. 2024, 14(20), 9487; https://doi.org/10.3390/app14209487 (registering DOI) - 17 Oct 2024
Abstract
This paper comprehensively analyzes advanced Fault Diagnosis and Prognosis (FDP) techniques employed in aerial and space agents such as satellites, spacecraft, and Unmanned Aerial Vehicles (UAVs). The critical engineering functions of fault diagnostics and prognosis, particularly the emerging field of fault prognosis, emphasize [...] Read more.
This paper comprehensively analyzes advanced Fault Diagnosis and Prognosis (FDP) techniques employed in aerial and space agents such as satellites, spacecraft, and Unmanned Aerial Vehicles (UAVs). The critical engineering functions of fault diagnostics and prognosis, particularly the emerging field of fault prognosis, emphasize the necessity for further advancement. Integrating these methodologies enriches the system’s capacity to diagnose faults in their early stages. Additionally, it enables the prediction of fault propagation and facilitates proactive maintenance to mitigate the risk of severe failure. This paper aims to introduce diverse FDP methods, followed by a discussion on their application and evolution within single and multisatellite/UAV systems. Throughout this review, eighty-five relevant works are analyzed and discussed and their evaluation metrics are expanded upon as well. Within the works analyzed in this review, it was found that data-driven methods constitute 54% and 7% of the methodologies utilized in single- and multiagent FDP, respectively, which underscores the rise of these methods in the field of single-agent FDP and their unexplored potential in multiagent condition monitoring. Finally, this review is brought to a close with a suggested classification scheme of the utilized methodologies in the field, a quantitative analysis of their contributions to the field, and remarks and mentions of the potential gaps in the area. Full article
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<p>Behavior of different fault types over time.</p>
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<p>FDP and fault-tolerant control schemes.</p>
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<p>Fault diagnosis strategy.</p>
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<p>Fault Diagnosis and Prognosis approaches.</p>
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<p>Fault diagnosis architectures: (<b>a</b>) centralized; (<b>b</b>) decentralized; (<b>c</b>) distributed.</p>
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<p>The review’s systematic search, screening, and analysis approach.</p>
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<p>Data augmentation for a sample image of a dog (the original image is available <a href="https://unsplash.com/photos/short-coated-brown-and-white-puppy-eoqnr8ikwFE" target="_blank">https://unsplash.com/photos/short-coated-brown-and-white-puppy-eoqnr8ikwFE</a> here) (accessed on 10 July 2024).</p>
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<p>An example of generating spectrograms from RW time series signals for use in image-based data-driven models.</p>
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<p>A schematic inspired by [<a href="#B98-applsci-14-09487" class="html-bibr">98</a>] of how a dynamic system abiding by a simple Markov process transitions between nominal and faulty modes.</p>
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<p>Compilation and classification of the methodologies utilized in the literature on satellite/UAV Fault Diagnosis and Prognosis.</p>
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<p>Breakdown of the methodologies used in the single-agent FDP literature.</p>
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<p>Breakdown of the methodologies used in the multiagent FDP literature.</p>
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21 pages, 7722 KiB  
Article
Transcriptomic Analysis During Olive Fruit Development and Expression Profiling of Fatty Acid Desaturase Genes
by Alicia Serrano, Judith García-Martín, Martín Moret, José Manuel Martínez-Rivas and Francisco Luque
Int. J. Mol. Sci. 2024, 25(20), 11150; https://doi.org/10.3390/ijms252011150 (registering DOI) - 17 Oct 2024
Abstract
The olive fruit is a drupe whose development and ripening takes several months from flowering to full maturation. During this period, several biochemical and physiological changes occur that affect the skin color, texture, composition, and size of the mesocarp. The final result is [...] Read more.
The olive fruit is a drupe whose development and ripening takes several months from flowering to full maturation. During this period, several biochemical and physiological changes occur that affect the skin color, texture, composition, and size of the mesocarp. The final result is a fruit rich in fatty acids, phenolic compounds, tocopherols, pigments, sterols, terpenoids, and other compounds of nutritional interest. In this work, a transcriptomic analysis was performed using flowers (T0) and mesocarp tissue at seven different stages during olive fruit development and ripening (T1–T7) of the ‘Picual’ cultivar. A total of 1755 genes overexpressed at any time with respect to the flowering stage were further analyzed. These genes were grouped into eight clusters based on their expression profile. The gene enrichment analysis revealed the most relevant biological process of every cluster. Highlighting the important role of hormones at very early stages of fruit development (T1, Cluster 1), whereas genes involved in fatty acid biosynthesis were relevant throughout the fruit developmental process. Hence, genes coding for different fatty acid desaturase (SAD, FAD2, FAD3, FAD4, FAD5, FAD6, and FAD7) enzymes received special attention. In particular, 26 genes coding for different fatty acid desaturase enzymes were identified in the ‘Picual’ genome, contributing to the improvement of the genome annotation. The expression pattern of these genes during fruit development corroborated their role in determining fatty acid composition. Full article
(This article belongs to the Special Issue Genomic and Transcriptomic Analysis of Olive (Olea europaea L.))
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<p>(<b>A</b>) Developmental stages collected for RNAseq analysis. (<b>B</b>) PCA plot showing the expression differences among olive fruit developing samples. Samples collected in triplicate: T0: flowers at full bloom, T1: fruits at 15 days after full blooming (AFB), T2: fruits at 1 month AFB, T3: fruits at 2 months AFB, T4: fruits at 3 months AFB, T5: fruits at 4 months AFB, T6: fruits at 5 months AFB, and T7: fruits at 6 months AFB.</p>
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<p>Differentially expressed genes throughout fruit development owing to the flowering stage. T0: flowers at full bloom, T1: 15 days after full blooming (AFB), T2: 1 month AFB, T3: 2 months AFB, T4: 3 months AFB, T5: 4 months AFB, T6: 5 months AFB, and T7: 6 months AFB.</p>
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<p>Cluster A. (<b>A</b>) Gene expression profile. Blue line represents the mean value of expression for the total genes in the group. Blue shadow represents the standard error of gene expression. Gray lines represent the expression of individual genes. The red horizontal line represents the threshold separating positive from negative expression levels. (<b>B</b>) The 20 most representative biological processes according to the FDR and fold enrichment values obtained in ShinyGO 0.80.</p>
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<p>Cluster B. (<b>A</b>) Gene expression profile. Blue line represents the mean value of expression for the total genes in the group. Blue shadow represents the standard error of gene expression. Gray lines represent the expression of individual genes. The red horizontal line represents the threshold separating positive from negative expression levels. (<b>B</b>) The 20 most representative biological processes according to the FDR and fold enrichment values obtained in ShinyGO 0.80.</p>
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<p>Cluster C. (<b>A</b>) Gene expression profile. Blue line represents the mean value of expression for the total genes in the group. Blue shadow represents the standard error of gene expression. Gray lines represent the expression of individual genes. The red horizontal line represents the threshold separating positive from negative expression levels. (<b>B</b>) The 20 most representative biological processes according to the FDR and fold enrichment values obtained in ShinyGO 0.80.</p>
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<p>Cluster D. (<b>A</b>) Gene expression profile. Blue line represents the mean value of expression for the total genes in the group. Blue shadow represents the standard error of gene expression. Gray lines represent the expression of individual genes. The red horizontal line represents the threshold separating positive from negative expression levels. (<b>B</b>) The 20 most representative biological processes according to the FDR and fold enrichment values obtained in ShinyGO 0.80.</p>
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<p>Cluster E. (<b>A</b>) Gene expression profile. Blue line represents the mean value of expression for the total genes in the group. Blue shadow represents the standard error of gene expression. Gray lines represent the expression of individual genes. The red horizontal line represents the threshold separating positive from negative expression levels. (<b>B</b>) The 20 most representative biological processes according to the FDR and fold enrichment values obtained in ShinyGO 0.80.</p>
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<p>Cluster F. (<b>A</b>) Gene expression profile. Blue line represents the mean value of expression for the total genes in the group. Blue shadow represents the standard error of gene expression. Gray lines represent the expression of individual genes. The red horizontal line represents the threshold separating positive from negative expression levels. (<b>B</b>) The 20 most representative biological processes according to the FDR and fold enrichment values obtained in ShinyGO 0.80.</p>
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<p>Cluster G. (<b>A</b>) Gene expression profile. Blue line represents the mean value of expression for the total genes in the group. Blue shadow represents the standard error of gene expression. Gray lines represent the expression of individual genes. The red horizontal line represents the threshold separating positive from negative expression levels. (<b>B</b>) The 20 most representative biological processes according to the FDR and fold enrichment values obtained in ShinyGO 0.80.</p>
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<p>Cluster H. (<b>A</b>) Gene expression profile. Blue line represents the mean value of expression for the total genes in the group. Blue shadow represents the standard error of gene expression. Gray lines represent the expression of individual genes. The red horizontal line represents the threshold separating positive from negative expression levels. (<b>B</b>) The 20 most representative biological processes according to the FDR and fold enrichment values obtained in ShinyGO 0.80.</p>
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<p>Expression of coding genes for isoforms of SAD enzyme during olive fruit development.</p>
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<p>Expression of coding genes for microsomal FAD enzymes during olive fruit development.</p>
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<p>Expression of coding genes for plastidial membrane-bound FAD enzymes during olive fruit development.</p>
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16 pages, 5268 KiB  
Article
Pathogenicity of Citrobacter freundii Causing Mass Mortalities of Macrobrachium rosenbergii and Its Induced Host Immune Response
by Anting Chen, Qieqi Qian, Xiaoyu Cai, Jia Yin, Yan Liu, Qi Dong, Xiaojian Gao, Qun Jiang and Xiaojun Zhang
Microorganisms 2024, 12(10), 2079; https://doi.org/10.3390/microorganisms12102079 (registering DOI) - 17 Oct 2024
Abstract
Citrobacter freundii is an opportunistic pathogen of freshwater aquatic animals, which severely restricts the sustainable development of the aquaculture industry. In this study, a dominant strain, named FSNM-1, was isolated from the hepatopancreas of diseased Macrobrachium rosenbergii. This strain was identified as [...] Read more.
Citrobacter freundii is an opportunistic pathogen of freshwater aquatic animals, which severely restricts the sustainable development of the aquaculture industry. In this study, a dominant strain, named FSNM-1, was isolated from the hepatopancreas of diseased Macrobrachium rosenbergii. This strain was identified as C. freundii based on a comprehensive analysis of its morphological, physiological, and biochemical features and molecular identification. Challenge experiments were conducted to assess the pathogenicity of C. freundii to M. rosenbergii. The results showed that the FSNM-1 strain had high virulence to M. rosenbergii with a median lethal dose (LD50) of 1.1 × 106 CFU/mL. Histopathological analysis revealed that C. freundii infection caused different degrees of inflammation in the hepatopancreas, gills, and intestines of M. rosenbergii. The detection of virulence-related genes revealed that the FSNM-1 strain carried colonization factor antigen (cfa1, cfa2), ureases (ureG, ureF, ureD, ureE), and outer membrane protein (ompX), and virulence factor detection showed that the FSNM-1 strain had lecithinase, amylase, lipase, gelatinase, and hemolysin activities but did not produce protease and DNase activities. To investigate the immune response of M. rosenbergii to C. freundii, the expression levels of ALF3, MyD88, SOD, proPO, TRAF6, and TNF immune-related genes were monitored at different points of time in the hepatopancreas, gills, intestines, and hemocytes of M. rosenbergii after infection. The results demonstrated a significant upregulation in the expression levels of the ALF3, MyD88, SOD, proPO, TRAF6, and TNF genes in M. rosenbergii at the early stage of C. freundii infection. This study highlights C. freundii as a major pathogen causing mass mortality in M. rosenbergii and provides valuable insights into its virulence mechanisms and the host’s immune response. Full article
(This article belongs to the Special Issue Pathogens and Aquaculture)
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<p>Pathogenicity of the FSNM-1 strain to <span class="html-italic">M. rosenbergii</span>.</p>
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<p>H&amp;E-stained histological sections of <span class="html-italic">M. rosenbergii</span> (bar = 100 μm). (<b>A</b>) Hepatopancreas of the control group; (<b>B</b>) Hepatopancreas of the test group, the arrow shows loss of the star-like shape of the lumen; (<b>C</b>) gills of the control group; (<b>D</b>) gills of the test group, * shows clubbing at the tip of the gill filaments; (<b>E</b>) Intestine of the control group; (<b>F</b>) Intestine of the test group.</p>
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<p>Electron micrograph of the FSNM-1 strain showing peri-flagellum (bar = 1 μm).</p>
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<p>Neighbor-joining (FSNM-1) phylogenetic tree based on partial <span class="html-italic">gyrB</span> gene sequences. Bootstrap values are shown beside the clades.</p>
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<p>Agarose (1%) gel electrophoresis of PCR virulence gene products. M: 2000 bp DNA marker, Lane 1: <span class="html-italic">cfa1</span>, 2: negative control, 3: <span class="html-italic">cfa2</span>, 4: negative control, 5: <span class="html-italic">ompX</span>, 6: negative control, 7: <span class="html-italic">ureD</span>, 8: negative control, 9: <span class="html-italic">ureG</span>, 10: negative control, 11: <span class="html-italic">ureE</span>, 12: negative control, 13: <span class="html-italic">ureF</span>, 14: negative control.</p>
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<p>Immune-related gene expression in hepatopancreas after <span class="html-italic">C. freundii</span> infection. (<b>A</b>) <span class="html-italic">ALF3</span>, (<b>B</b>) <span class="html-italic">MyD88</span>, (<b>C</b>) <span class="html-italic">TNF</span>, (<b>D</b>) <span class="html-italic">TRAF6</span>, (<b>E</b>) <span class="html-italic">SOD</span>, (<b>F</b>) <span class="html-italic">proPO</span>. Data presented as mean ± SD, ** <span class="html-italic">p</span> &lt; 0.01.</p>
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<p>Immune-related gene expression in gills after <span class="html-italic">C. freundii</span> infection. (<b>A</b>) <span class="html-italic">ALF3</span>, (<b>B</b>) <span class="html-italic">MyD88</span>, (<b>C</b>) <span class="html-italic">TNF</span>, (<b>D</b>) <span class="html-italic">TRAF6</span>, (<b>E</b>) <span class="html-italic">SOD</span>, (<b>F</b>) <span class="html-italic">proPO</span>. Data presented as mean ± SD, ** <span class="html-italic">p</span> &lt; 0.01.</p>
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<p>Immune-related gene expression in intestines after <span class="html-italic">C. freundii</span> infection. (<b>A</b>) <span class="html-italic">ALF3</span>, (<b>B</b>) <span class="html-italic">MyD88</span>, (<b>C</b>) <span class="html-italic">TNF</span>, (<b>D</b>) <span class="html-italic">TRAF6</span>, (<b>E</b>) <span class="html-italic">SOD</span>, (<b>F</b>) <span class="html-italic">proPO</span>. Data presented as mean ± SD, ** <span class="html-italic">p</span> &lt; 0.01.</p>
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<p>Immune-related gene expression in hemocytes after <span class="html-italic">C. freundii</span> infection. (<b>A</b>) <span class="html-italic">ALF3</span>, (<b>B</b>) <span class="html-italic">MyD88</span>, (<b>C</b>) <span class="html-italic">TNF</span>, (<b>D</b>) <span class="html-italic">TRAF6</span>, (<b>E</b>) <span class="html-italic">SOD</span>, (<b>F</b>) <span class="html-italic">proPO</span>. Data presented as mean ± SD, ** <span class="html-italic">p</span> &lt; 0.01.</p>
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11 pages, 2397 KiB  
Article
Association of Cardiovascular Risk Factors and Coronary Calcium Burden with Epicardial Adipose Tissue Volume Obtained from PET–CT Imaging in Oncological Patients
by Carmela Nappi, Andrea Ponsiglione, Carlo Vallone, Roberto Lepre, Luigi Basile, Roberta Green, Valeria Cantoni, Ciro Gabriele Mainolfi, Massimo Imbriaco, Mario Petretta and Alberto Cuocolo
J. Cardiovasc. Dev. Dis. 2024, 11(10), 331; https://doi.org/10.3390/jcdd11100331 (registering DOI) - 17 Oct 2024
Abstract
Whole-body positron emission tomography (PET)–computed tomography (CT) imaging performed for oncological purposes may provide additional parameters such as the coronary artery calcium (CAC) and epicardial adipose tissue (EAT) volume with cost-effective prognostic information in asymptomatic people beyond traditional cardiovascular risk factors. We evaluated [...] Read more.
Whole-body positron emission tomography (PET)–computed tomography (CT) imaging performed for oncological purposes may provide additional parameters such as the coronary artery calcium (CAC) and epicardial adipose tissue (EAT) volume with cost-effective prognostic information in asymptomatic people beyond traditional cardiovascular risk factors. We evaluated the feasibility of measuring the CAC score and EAT volume in cancer patients without known coronary artery disease (CAD) referred to whole-body 18F-FDG PET–CT imaging, regardless of the main clinical problem. We also investigated the potential relationships between traditional cardiovascular risk factors and CAC with EAT volume. A total of 109 oncological patients without overt CAD underwent whole-body PET–CT imaging with 18F-fluorodeoxyglucose (FDG). Unenhanced CT images were retrospectively viewed for CAC and EAT measurements on a dedicated platform. Overall, the mean EAT volume was 99 ± 49 cm3. Patients with a CAC score ≥ 1 were older than those with a CAC = 0 (p < 0.001) and the prevalence of hypertension was higher in patients with detectable CAC as compared to those without (p < 0.005). The EAT volume was higher in patients with CAC than in those without (p < 0.001). For univariable age, body mass index (BMI), hypertension, and CAC were associated with increasing EAT values (all p < 0.005). However, the correlation between the CAC score and EAT volume was weak, and in multivariable analysis only age and BMI were independently associated with increased EAT (both p < 0.001), suggesting that potential prognostic information on CAC and EAT is not redundant. This study demonstrates the feasibility of a cost-effective assessment of CAC scores and EAT volumes in oncological patients undergoing whole-body 18F-FDG PET–CT imaging, enabling staging cancer disease and atherosclerotic burden by a single test already included in the diagnostic work program, with optimization of the radiation dose and without additional costs. Full article
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<p>Correlation between epicardial adipose tissue (EAT) volume and coronary artery calcium (CAC) score.</p>
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<p>Case example of a 20-year-old man with Hodgkin’s lymphoma.</p>
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<p>Case example of an 80-year-old man with colorectal cancer.</p>
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17 pages, 1994 KiB  
Article
Notes on Modified Planar Kelvin–Stuart Models: Simulations, Applications, Probabilistic Control on the Perturbations
by Nikolay Kyurkchiev, Tsvetelin Zaevski, Anton Iliev, Vesselin Kyurkchiev and Asen Rahnev
Axioms 2024, 13(10), 720; https://doi.org/10.3390/axioms13100720 (registering DOI) - 17 Oct 2024
Abstract
In this paper, we propose a new modified planar Kelvin–Stuart model. We demonstrate some modules for investigating the dynamics of the proposed model. This will be included as an integral part of a planned, much more general Web-based application for scientific computing. Investigations [...] Read more.
In this paper, we propose a new modified planar Kelvin–Stuart model. We demonstrate some modules for investigating the dynamics of the proposed model. This will be included as an integral part of a planned, much more general Web-based application for scientific computing. Investigations in light of Melnikov’s approach are considered. Some simulations and applications are also presented. The proposed new modifications of planar Kelvin–Stuart models contain many free parameters (the coefficients gi,i=1,2,,N), which makes them attractive for use in engineering applications such as the antenna feeder technique (a possible generating and simulating of antenna factors) and the theory of approximations (a possible good approximation of a given electrical stage). The probabilistic control of the perturbations is discussed. Full article
(This article belongs to the Special Issue Differential Equations and Related Topics, 2nd Edition)
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Figure 1
<p>The orbits <math display="inline"><semantics> <mrow> <mo>(</mo> <msub> <mi>x</mi> <mn>0</mn> </msub> <mrow> <mo>(</mo> <mi>t</mi> <mo>)</mo> </mrow> <mo>,</mo> <msub> <mi>y</mi> <mn>0</mn> </msub> <mrow> <mo>(</mo> <mi>t</mi> <mo>)</mo> </mrow> <mo>)</mo> </mrow> </semantics></math>.</p>
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<p>(<b>a</b>) The solutions of the system (3); (<b>b</b>) phase space (Example 1).</p>
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<p>(<b>a</b>) The solutions of the system (3); (<b>b</b>) phase space (Example 2).</p>
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<p>(<b>a</b>) The solutions of the system (3); (<b>b</b>) phase space (Example 3).</p>
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<p>(<b>a</b>) The solutions of the system (3); (<b>b</b>) phase space (Example 4).</p>
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<p>(<b>a</b>) The solutions of the system (3); (<b>b</b>) phase space (Example 5).</p>
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<p>A typical antenna factor (from Example 4).</p>
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<p>The approximation of the electrical stage (red) using <math display="inline"><semantics> <mrow> <mi>x</mi> <mo>(</mo> <mi>t</mi> <mo>)</mo> </mrow> </semantics></math> component of the solution of the differential system (3).</p>
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<p>The approximation of the cut function (red) using <math display="inline"><semantics> <mrow> <mi>x</mi> <mo>(</mo> <mi>t</mi> <mo>)</mo> </mrow> </semantics></math> component of the solution of the differential system (3).</p>
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<p>(<b>a</b>) The solutions of the system (5); (<b>b</b>) phase space (Example 6).</p>
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<p>The Melnikov function (7) for fixed <math display="inline"><semantics> <mrow> <mi>N</mi> <mo>=</mo> <mn>1</mn> </mrow> </semantics></math> and <math display="inline"><semantics> <mrow> <msub> <mi>g</mi> <mn>1</mn> </msub> <mo>=</mo> <mn>1</mn> </mrow> </semantics></math>, in the cases when: <math display="inline"><semantics> <mrow> <mi>A</mi> <mo>=</mo> <mn>0.9</mn> </mrow> </semantics></math>, <math display="inline"><semantics> <mrow> <mi>ω</mi> <mo>=</mo> <mn>0.8</mn> </mrow> </semantics></math> (red); <math display="inline"><semantics> <mrow> <mi>A</mi> <mo>=</mo> <mn>0.7</mn> </mrow> </semantics></math>, <math display="inline"><semantics> <mrow> <mi>ω</mi> <mo>=</mo> <mn>1.8</mn> </mrow> </semantics></math> (blue).</p>
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<p>Solving <math display="inline"><semantics> <msub> <mi>I</mi> <mi>n</mi> </msub> </semantics></math> using CAS Mathematica.</p>
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<p>Solving <math display="inline"><semantics> <msubsup> <mi>I</mi> <mi>n</mi> <mo>*</mo> </msubsup> </semantics></math> using CAS Mathematica.</p>
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<p>The Melnikov functions (8) for fixed <math display="inline"><semantics> <mrow> <mi>N</mi> <mo>=</mo> <mn>2</mn> </mrow> </semantics></math> and when: <math display="inline"><semantics> <mrow> <mi>A</mi> <mo>=</mo> <mn>0.2</mn> </mrow> </semantics></math>, <math display="inline"><semantics> <mrow> <mi>ω</mi> <mo>=</mo> <mn>0.95</mn> </mrow> </semantics></math>, <math display="inline"><semantics> <mrow> <msub> <mi>g</mi> <mn>1</mn> </msub> <mo>=</mo> <mn>0.8</mn> </mrow> </semantics></math>, <math display="inline"><semantics> <mrow> <msub> <mi>g</mi> <mn>2</mn> </msub> <mo>=</mo> <mn>0.9</mn> </mrow> </semantics></math> (red); <math display="inline"><semantics> <mrow> <mi>A</mi> <mo>=</mo> <mn>0.3</mn> </mrow> </semantics></math>, <math display="inline"><semantics> <mrow> <mi>ω</mi> <mo>=</mo> <mn>0.8</mn> </mrow> </semantics></math>, <math display="inline"><semantics> <mrow> <msub> <mi>g</mi> <mn>1</mn> </msub> <mo>=</mo> <mn>0.7</mn> </mrow> </semantics></math>, <math display="inline"><semantics> <mrow> <msub> <mi>g</mi> <mn>2</mn> </msub> <mo>=</mo> <mn>0.6</mn> </mrow> </semantics></math> (blue); <math display="inline"><semantics> <mrow> <mi>A</mi> <mo>=</mo> <mn>0.35</mn> </mrow> </semantics></math>, <math display="inline"><semantics> <mrow> <mi>ω</mi> <mo>=</mo> <mn>0.7</mn> </mrow> </semantics></math>, <math display="inline"><semantics> <mrow> <msub> <mi>g</mi> <mn>1</mn> </msub> <mo>=</mo> <mn>0.6</mn> </mrow> </semantics></math>, <math display="inline"><semantics> <mrow> <msub> <mi>g</mi> <mn>2</mn> </msub> <mo>=</mo> <mn>0.5</mn> </mrow> </semantics></math> (green).</p>
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<p>Oscillators based on the gamma and beta distributions.</p>
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<p>Oscillators based on the gamma and beta distributions.</p>
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<p>A typical antenna factor (from Example 7).</p>
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<p>A typical antenna factor (from Example 8).</p>
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<p>A typical antenna factor (from Example 9).</p>
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<p>A typical antenna factor (from Example 10).</p>
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<p>A typical Melnikov antenna array (Example 11).</p>
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<p>A typical Melnikov antenna array (Example 12).</p>
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26 pages, 1960 KiB  
Article
Fast CU Partition Decision Algorithm Based on Bayesian and Texture Features
by Erlin Tian, Yifan Yang and Qiuwen Zhang
Electronics 2024, 13(20), 4082; https://doi.org/10.3390/electronics13204082 (registering DOI) - 17 Oct 2024
Abstract
As internet speeds increase and user demands for video quality grow, video coding standards continue to evolve. H.266/Versatile Video Coding (VVC), as the new generation of video coding standards, further improves compression efficiency but also brings higher computational complexity. Despite the significant advancements [...] Read more.
As internet speeds increase and user demands for video quality grow, video coding standards continue to evolve. H.266/Versatile Video Coding (VVC), as the new generation of video coding standards, further improves compression efficiency but also brings higher computational complexity. Despite the significant advancements VVC has made in compression ratio and video quality, the introduction of new coding techniques and complex coding unit (CU) partitioning methods have also led to increased encoding complexity. This complexity not only extends encoding time but also increases hardware resource consumption, limiting the application of VVC in real-time video processing and low-power devices.To alleviate the encoding complexity of VVC, this paper puts forward a Bayesian and texture-feature-based fast splitting algorithm for coding intraframe bloc of VVC, which aims to reduce unnecessary computational steps, enhance encoding efficiency, and maintain video quality as much as possible. In the stage of rapid coding, the video frames are coded by the original VVC test model (VTM), and Joint Rough Mode Decision (JRMD) evaluation cost is used to update the parameter in the Bayesian algorithm to come and set the two thresholds to judge whether the current coding block continues to be split or not. Then, for coding blocks larger than those satisfying the above threshold conditions, the predominant direction of the texture within the coding block is ascertained by calculating the standard deviations along both the horizontal and vertical axes so as to skip some unnecessary splits in the current coding block patterns. The findings from our experiments demonstrate that our proposed approach improves the encoding rate by 1.40% on average, and the execution time of the encoder has been reduced by 49.50%. The overall algorithm has optimized the VVC intraframe coding technology and reduced the coding complexity of VVC. Full article
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<p>CTU partition map.</p>
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<p>Fitting of Gaussian distribution for the JRMD number of CUs.</p>
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<p>Gaussian distribution fitting for JRMD number counts and CUs.</p>
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<p>Texture characteristics of 64 × 64 CUs.</p>
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<p>Percentage of the prediction accuracy at different threshold values.</p>
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<p>Overall algorithmic flow framework.</p>
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<p>Comparison of VTM 10.0 standard encoder RD curves with proposed algorithm on each test sequence.</p>
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<p>Contrasting the mean BDBR and TS values across various algorithms.</p>
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<p>RD curves of overall scheme performance.</p>
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