Search Results (3,233)

The skeleton structure composed of mineral aggregates is the main body to bear and transfer external loading in asphalt mixtures. To investigate the loading transfer mechanism of the mineral aggregate skeleton, the uniaxial penetration test and Discrete Element Method (DEM) were conducted for the Mineral Aggregate Mixture (MAM) to analyze its mechanical behavior. The three-dimensional strong force chain (SFC) was identified and evaluated based on the proposed recognition criterion and evaluation indices. The results indicate that 4.75 mm should be the boundary to distinguish the coarse and fine aggregates. The skeleton composed of aggregates located on SFCs has better bearing and transferring loading capacity due to its SFC number, average length, and total length decreasing with an increase in the aggregate size. Compared to SMA-16 and OGFC-16, AC-16 exhibits a higher number and total length of its SFC, a smaller average length of its SFC, and a lower average strength of its SFC. Consequently, AC-16 has a lower bearing and transferring loading capacity than that of SMA-16 and OGFC-16. In addition, approximately 90% of SFCs can only transfer external loading downward through 3–5 aggregates. The average direction angle of the SFC formed by fine aggregates is significantly higher than those formed by coarse aggregates. This indicates that the load transfer range of MAM composed of fine aggregates is noticeably larger, leading to lower loading transfer efficiency. Full article

Aiming at the problems of small stems and irregular contours of bitter gourd, which lead to difficult and inaccurate location of picking points in the picking process of mechanical arms, this paper proposes an improved YOLOv5-seg instance segmentation algorithm with a coordinate attention (CA) mechanism module, and combines it with a refinement algorithm to identify and locate the picking points of bitter gourd. Firstly, the improved algorithm model was used to identify and segment bitter gourd and melon stems. Secondly, the melon stem mask was extracted, and the thinning algorithm was used to refine the skeleton of the extracted melon stem mask image. Finally, a skeleton refinement graph of bitter gourd stem was traversed, and the midpoint of the largest connected region was selected as the picking point of bitter gourd. The experimental results show that the prediction precision (P), precision (R) and mean average precision (mAP) of the improved YOLOv5-seg model in object recognition were 98.04%, 97.79% and 98.15%, respectively. Compared with YOLOv5-seg, the P, R and mA values were increased by 2.91%, 4.30% and 1.39%, respectively. In terms of object segmentation, mask precision (P(M)) was 99.91%, mask recall (R(M)) 99.89%, and mask mean average precision (mAP(M)) 99.29%. Compared with YOLOv5-seg, the P(M), R(M), and mAP(M) values were increased by 6.22%, 7.81%, and 5.12%, respectively. After testing, the positioning error of the three-dimensional coordinate recognition of bitter gourd picking points was X-axis = 7.025 mm, Y-axis =5.6135 mm, and Z-axis = 11.535 mm, and the maximum allowable error of the cutting mechanism at the end of the picking manipulator was X-axis = 30 mm, Y-axis = 24.3 mm, and Z-axis = 50 mm. Therefore, this results of study meet the positioning accuracy requirements of the cutting mechanism at the end of the manipulator. The experimental data show that the research method in this paper has certain reference significance for the accurate identification and location of bitter gourd picking points. Full article

This paper studies the influence of the staged particle sieving-out method on the seepage characteristics in loose sandstone heap leaching. The staged sieving out of ore sample particles was conducted according to particle size, and ground pressure was applied to them. Subsequently, parameters such as the permeability, particle distribution, and pore distribution characteristics of the rock samples were obtained to investigate the influence of the staged particle sieving-out method on the seepage effect of loose sandstone heap leaching. The results indicate that sieving out particles smaller than 0.15 mm can significantly reduce the probability of hole blockage and increase the overall pore size, greatly enhancing permeability. Sieving out particles with sizes between 0.15 mm and 1.2 mm can result in the loss of skeleton particles, reducing the amount of flow channels and thereby decreasing permeability. Sieving out particles larger than 1.2 mm can reduce the overall particle size of rock samples, improve strength and pressure stability, and help maintain permeability. In the surface heap leaching of loose sandstone ore, by sieving out particles smaller than 0.15 mm during deep heap construction and sieving out particles larger than 1.2 mm during mid-level heap construction, and by using vat leaching for sieved-out particles, the seepage effect of the ore heap can be significantly optimized, and complete utilization of resources can be ensured. Full article

We present a technique for the detection of vertebrate skeletons buried at shallow depths through the use of a ground-penetrating radar (GPR). The technique is based on the acquisition of high-resolution data by medium-to-high frequency GPR antennas and the analysis of the radar profiles by a new forward modelling method that is applied on a set of representative traces. This approach allows us to obtain synthetic traces that can be used to build detailed reflectivity diagrams that plot spikes with a distinct amplitude and polarity for each reflector in the ground. The method was tested in a controlled experiment performed at the top of Cerro Los Quesos, one of the most fossiliferous localities in the Ica Desert of Peru. We acquired GPR data at the location of a partially buried fossil skeleton of a large whale and analyzed the reflections associated with the bones using the new technique, determining the possible signature of vertebrae, ribs, the cranium (including the rostrum), and mandibles. Our results show that the technique is effective in the mapping of buried structures, particularly in the detection of tiny features, even below the classical (Ricker and Rayleigh) estimates of the vertical resolution of the antenna in civil engineering and forensic applications. Full article

Excessive consumption of natural resources to meet the growing demands of building and infrastructure projects has put enormous stress on these resources. On the other hand, a significant quantity of soil is excavated for development activities across the globe and is usually treated as waste material. This study explores the potential of excavated soils in the Brittany region of France for its reuse as earthen construction materials. Characterization of soil recovered from building sites was carried out to classify the soils and observe their suitability for earthen construction materials. These characteristics include mainly Atterberg limits, granulometry, organic matter and optimum moisture content. Soil samples were separated into fine and coarse particles through wet sieving. The percentage of fines (particles smaller than 0.063 mm) in studied soil samples range from 28% to 65%. The methylene blue value (MBV) for Lorient, Bruz and Polama soils is 1, 1.2 and 1.2 g/100 g, and French classification (Guide de terrassements des remblais et des couches de forme; GTR) of soil samples is A1, B5 and A1, respectively. The washing of soils with lower fine content helps to recover excellent-quality sand and gravel, which are a useful and precious resource. However, residual fine particles are a waste material. In this study, three soil formulations were used for manufacturing earth blocks. These formulations include raw soil, fines and restructured soil. In restructured soil, a fine fraction of soil smaller than 0.063 mm was mixed with 15% recycled sand. Restructuring of soil fine particles helps to improve soil matrix composition and suitability for earth bricks. Compressed-earth blocks of 4 × 4 × 16 cm were manufactured at a laboratory scale for flexural strength testing by using optimum molding moisture content and compaction through Proctor normal energy. Compressive strength tests were performed on cubic blocks of size 4 × 4 × 4 cm. Mechanical testing of bricks showed that bricks with raw soil had higher resistance with a maximum of 3.4 MPa for Lorient soil. Removal of coarse particles from soil decreased the strength of bricks considerably. Restructuring of fines with recycled sand improves their granular skeleton and increases the compressive strength and durability of bricks. Full article

As the main form of new urbanization, the coordinated development of cities in metropolitan areas requires reliable and efficient rail transit skeleton support. However, in the rapid development of metropolitan areas, the layout and analysis of multi-level rail transit systems have a certain lag. Taking the Xi’an metropolitan area as an example, this study analyzes the comprehensive accessibility and resilience of the multi-level rail transit network, and proposes an expansion plan accordingly. The traffic analysis zone (TAZ) is divided by towns and streets, and the relationship between points of interest (POIs) and the regional average level is analyzed using DEA. The improved weighted average travel time model is built with the analysis results as regional weights; a site selection model based on multiple construction influencing factors is proposed, and four expansion plans, namely, economic optimal, environmental optimal, transport optimal, and integrated optimal, are designed. The peak passenger flow scenario and the “failure–reparation” scenario during the entire operation period are designed to analyze the resilience of four plans, and the resilience is quantified by the elasticity curve of the maximum connected subgraph ratio (MCSR) changing over time. The research results show that the transport optimal plan has the best comprehensive accessibility and resilience, reducing travel costs in Houzhenzi Town, which has the worst accessibility, by 34%. The expansion model and evaluation method in this study can provide an empirical example for the development of other metropolitan areas and provide a reasonable benchmark and guidance for the development of multi-level rail transit networks in future urban areas. Full article

Protein adsorption behavior was examined on poly(N-isopropylacrylamide-co-sodium methacrylate)-based hydrogels at different temperatures: 5, 20, and 37 °C, and pH: 4.5, 7, and 9.2. The hydrogels, whose covalent skeleton contains pendant anionic units due to the presence of the sodium methacrylate co-monomer, exhibited both thermo- and pH-sensitivity with different extents, which depended on the content of ionizable moieties and the cross-linker density. The hydrogel composition, temperature, and pH influenced the zeta potential of the hydrogels and their swelling properties. The proteins selected for the study, i.e., bovine serum albumin (BSA), ovalbumin (OVA), lysozyme (LYZ), and a monoclonal antibody (mAb2), differed in their aminoacidic composition and conformation, thus in isoelectric point, molecular weight, electrostatic charge, and hydrophobicity. Therefore, the response of their adsorption behavior to changes in the solution properties and the hydrogel composition was different. LYZ exhibited the strongest adsorption of all proteins with a maximum at pH 7 (189.5 $\mathrm{m}\mathrm{g} {\mathrm{g}}_{gel}^{-1}$); adsorption of BSA and OVA reached maximum at pH 4.5 (24.4 and 23.5 $\mathrm{m}\mathrm{g} {\mathrm{g}}_{gel}^{-1}$), whereas mAb2 was strongly adsorbed at 9.2 (21.7 $\mathrm{m}\mathrm{g} {\mathrm{g}}_{gel}^{-1}$). This indicated the possibility of using the hydrogels for pH-mediated separation of proteins differing in charge under mild conditions in a water-rich environment of both the liquid solution and the adsorbed phase. The adsorption affinity of all proteins increased with temperature, which was attributed to the synergistic effects of attractive electrostatic and hydrophobic interactions. That effect was particularly marked for mAb2, for which the temperature change from 5 to 37 °C caused a twentyfold increase in adsorption. In all cases, the proteins could be released from the hydrogel surface by a reduction in temperature, an increase in pH, or a combination of both. This allows for the elimination of the use of salt solution as a desorbing agent, whose presence renders the recycling of buffering solutions difficult. Full article

Yak behavior is a valuable indicator of their welfare and health. Information about important statuses, including fattening, reproductive health, and diseases, can be reflected and monitored through several indicative behavior patterns. In this study, an improved YOLOv7-pose model was developed to detect six yak behavior patterns in real time using labeled yak key-point images. The model was trained using labeled key-point image data of six behavior patterns including walking, feeding, standing, lying, mounting, and eliminative behaviors collected from seventeen 18-month-old yaks for two weeks. There were another four YOLOv7-pose series models trained as comparison methods for yak behavior pattern detection. The improved YOLOv7-pose model achieved the best detection performance with precision, recall, mAP0.5, and mAP0.5:0.95 of 89.9%, 87.7%, 90.4%, and 76.7%, respectively. The limitation of this study is that the YOLOv7-pose model detected behaviors under complex conditions, such as scene variation, subtle leg postures, and different light conditions, with relatively lower precision, which impacts its detection performance. Future developments in yak behavior pattern detection will amplify the simple size of the dataset and will utilize data streams like optical and video streams for real-time yak monitoring. Additionally, the model will be deployed on edge computing devices for large-scale agricultural applications. Full article

(1) Background: This study investigates the resting-state brain characteristics of skeleton athletes compared to healthy age-matched non-athletes, using resting-state fMRI to investigate long-term skeleton-training-related changes in the brain. (2) Methods: Eleven skeleton athletes and twenty-three matched novices with no prior experience with skeleton were recruited. Amplitude of low-frequency fluctuation (ALFF) and seed-based functional connectivity analyses were explored to investigate resting-state functional magnetic resonance imaging (rs-fMRI) data, aiming to elucidate differences in resting-state brain function between the two groups. (3) Results: Compared to the control group, skeleton athletes exhibited significantly higher ALFF in the left fusiform, left inferior temporal gyrus, right inferior frontal gyrus, left middle temporal gyrus, left and right insula, left Rolandic operculum, left inferior frontal gyrus, and left superior temporal gyrus. Skeleton athletes exhibit stronger functional connectivity in brain regions associated with cognitive and motor control (superior frontal gyrus, insula), as well as those related to reward learning (putamen), visual processing (precuneus), spatial cognition (inferior parietal), and emotional processing (amygdala), during resting-state brain function. (4) Conclusions: The study contributes to understanding how motor training history shapes skeleton athletes’ brains, which have distinct neural characteristics compared to the control population, indicating potential adaptations in brain function related to their specialized training and expertise in the sport. Full article

The axial force will be altered as a result of the overturning influence exerted by both horizontal and vertical seismic events, as well as the secondary effects induced by gravitational loads. The variation of the axial force will greatly affect the seismic performance of reinforced concrete (RC) columns, thus warranting close attention. This paper proposes a hysteresis model of RC columns considering the cumulative damage effect under the action of variable axial force. First, three groups of cyclic loading tests were performed across three distinct groups. Subsequently, numerical analysis models were constructed, employing fiber-based finite element methods. Furthermore, according to the test and finite element simulation results, the existing damage value was modified to describe the degradation of the stiffness and load-bearing capacity. Next, through a regression analysis, the skeleton curve was established. Finally, the hysteresis behavior under the influence of variable axial load was ascertained. The results, when compared with the experimental data, show that the proposed hysteresis model can accurately describe the seismic performance of RC columns under the influence of variable axial force. Full article

The design and development of fluorescent materials for detecting cancer-related enzymes are crucial for cancer diagnosis and treatment. Herein, we present a substituted rhodamine derivative for the chromogenic and fluorogenic detection of the cancer-relevant enzyme γ-glutamyltranspeptidase (GGT). Initially, the probe is non-chromic and non-emissive due to its spirolactam form, which hinders extensive electronic delocalization over broader pathway. However, selective enzymatic cleavage of the side-coupled group triggers spirolactam ring opening, resulting in electronic flow across the rhodamine skeleton, and reduces the band gap for low-energy electronic transitions. This transformation turns the reaction mixture from colorless to intense pink, with prominent UV and fluorescence bands. The sensor’s selectivity was tested against various human enzymes, including urease, alkaline phosphatase, acetylcholinesterase, tyrosinase, and cyclooxygenase, and showed no response. Absorption and fluorescence titration analyses of the probe upon incremental addition of GGT into the probe solution revealed a consistent increase in both absorption and emission spectra, along with intensified pink coloration. The cellular toxicity of the receptor was evaluated using the MTT assay, and bioimaging analysis was performed on BHK-21 cells, which produced bright red fluorescence, demonstrating the probe’s excellent cell penetration and digestion capabilities for intracellular analytical detection. Molecular docking results supported the fact that probe-4 made stable interactions with the GGT active site residues. Full article

Garcinia gummi-gutta, commonly known as Garcinia cambogia (syn.), is a popular traditional herbal medicine known for its role in treating obesity, and has been incorporated into several nutraceuticals globally for this purpose. The fruit rind is also used as a food preservative and a condiment because of its high content of hydroxycitric acid, which imparts a sharp, sour flavour. This review highlights the major bioactive compounds present in the tree Garcinia gummi-gutta, with particular emphasis on (2S, 3S)-tetrahydro-3-hydroxy-5-oxo-2,3-furan dicarboxylic acid, commonly referred to as garcinia acid. This acid can be isolated in large amounts through a simple procedure. Additionally, it explores the synthetic transformations of garcinia acid into biologically potent and functionally useful enantiopure compounds, a relatively under-documented area in the literature. This acid, with its six-carbon skeleton, a γ-butyrolactone moiety, and two chiral centres bearing chemically amenable functional groups, offers a versatile framework as a chiron for the construction of diverse molecules of both natural and synthetic origin. The synthesis of chiral 3-substituted and 3,4-disubstituted pyrrolidine-2,5-diones, analogues of the Quararibea metabolite—a chiral enolic-γ-lactone; the concave bislactone skeletons of fungal metabolites (+)-avenaciolide and (−)-canadensolide; the structural skeletons of the furo[2,3-b]furanol part of the anti-HIV drug Darunavir; (−)-tetrahydropyrrolo[2,1-a]isoquinolinones, an analogue of (−)-crispine A; (−)-hexahydroindolizino[8,7-b]indolones, an analogue of the naturally occurring (−)-harmicine; and furo[2,3-b]pyrroles are presented here. Full article

Background/Objectives: The superscan pattern is a characteristic finding on bone scintigraphy, associated with a variety of metabolic bone diseases, malignancies, and other conditions. This pattern is characterized by a diffuse and intense uptake of radiotracer throughout the entire skeleton. Despite being a relatively rare finding, the superscan pattern can have significant clinical implications. Methods: This comprehensive review summarizes the available literature on the superscan pattern, focusing on its pathophysiology, clinical significance, and differential diagnoses. Relevant studies and case reports were analyzed to outline the diagnostic challenges associated with the interpretation of bone scintigraphy featuring the superscan pattern. Results: The literature highlights the clinical significance of the superscan pattern in various metabolic and oncologic conditions. Misinterpretation of this pattern can lead to diagnostic challenges, especially in distinguishing it from other pathologic conditions. Differential diagnosis remains crucial in the accurate interpretation and subsequent management of patients with this finding. Conclusions: This review provides a comprehensive overview of the superscan pattern on bone scintigraphy, aiming to assist clinicians in recognizing and managing this rare yet clinically important finding. Full article

Shear stress is one of the major hemodynamic forces acting on the endothelium. However, it is not well known how endothelial cells (EC) respond mechanically to these stimuli in vivo. Here we investigated whether changes in biomechanics properties and shear stress could increase cell susceptibility to injury, contributing to vascular fragility. We surgically implanted a shear stress modifier device on the carotid artery of ApoE-knockout mice (ApoE^−/−), which, due to its shape, causes a gradual stenosis in the vessel, resulting in distinct shear stress patterns. Our data show actin fibers accumulation in areas with higher lipid deposition in ApoE^−/−, indicating that dyslipidemia might interfere with EC actin cytoskeleton organization. We also showed that both shear stress and dyslipidemia were important for EC susceptibility to injury. Furthermore, lysosomal distribution, an important organelle for plasma membrane repair, was altered in ApoE^−/−, which could compromise EC’s ability to repair from damage. Therefore, dyslipidemia and variations in shear stress patterns not only affect cellular mechanics by compromising the actin cytoskeleton organization, but also enhance cell susceptibility to injury and alter vesicle trafficking in vascular cells. This may likely contribute to vascular fragility and thus to the initial steps of atherosclerosis development. Full article

The estimation of vessels’ centerlines is a critical step in assessing the geometry of the vessel, the topological representation of the vessel tree, and vascular network visualization. In this research, we present a novel method for obtaining geometric parameters from peripheral arteries in 3D medical binary volumes. Our approach focuses on centerline extraction, which yields smooth and robust results. The procedure starts with a segmented 3D binary volume, from which a distance map is generated using the Euclidean distance transform. Subsequently, a skeleton is extracted, and seed points and endpoints are identified. A search methodology is used to derive the best path on the skeletonized 3D binary array while tracking from the goal points to the seed point. We use the distance transform to calculate the distance between voxels and the nearest vessel surface, while also addressing bifurcations when vessels divide into multiple branches. The proposed method was evaluated on 22 real cases and 10 synthetically generated vessels. We compared our method to different state-of-the-art approaches and demonstrated its better performance. The proposed method achieved an average error of 1.382 mm with real patient data and 0.571 mm with synthetic data, both of which are lower than the errors obtained by other state-of-the-art methodologies. This extraction of the centerline facilitates the estimation of multiple geometric parameters of vessels, including radius, curvature, and length. Full article