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18 pages, 9570 KiB  
Article
A Depth Awareness and Learnable Feature Fusion Network for Enhanced Geometric Perception in Semantic Correspondence
by Fazeng Li, Chunlong Zou, Juntong Yun, Li Huang, Ying Liu, Bo Tao and Yuanmin Xie
Sensors 2024, 24(20), 6680; https://doi.org/10.3390/s24206680 (registering DOI) - 17 Oct 2024
Abstract
Deep learning is becoming the most widely used technology for multi-sensor data fusion. Semantic correspondence has recently emerged as a foundational task, enabling a range of downstream applications, such as style or appearance transfer, robot manipulation, and pose estimation, through its ability to [...] Read more.
Deep learning is becoming the most widely used technology for multi-sensor data fusion. Semantic correspondence has recently emerged as a foundational task, enabling a range of downstream applications, such as style or appearance transfer, robot manipulation, and pose estimation, through its ability to provide robust correspondence in RGB images with semantic information. However, current representations generated by self-supervised learning and generative models are often limited in their ability to capture and understand the geometric structure of objects, which is significant for matching the correct details in applications of semantic correspondence. Furthermore, efficiently fusing these two types of features presents an interesting challenge. Achieving harmonious integration of these features is crucial for improving the expressive power of models in various tasks. To tackle these issues, our key idea is to integrate depth information from depth estimation or depth sensors into feature maps and leverage learnable weights for feature fusion. First, depth information is used to model pixel-wise depth distributions, assigning relative depth weights to feature maps for perceiving an object’s structural information. Then, based on a contrastive learning optimization objective, a series of weights are optimized to leverage feature maps from self-supervised learning and generative models. Depth features are naturally embedded into feature maps, guiding the network to learn geometric structure information about objects and alleviating depth ambiguity issues. Experiments on the SPair-71K and AP-10K datasets show that the proposed method achieves scores of 81.8 and 83.3 on the percentage of correct keypoints (PCK) at the 0.1 level, respectively. Our approach not only demonstrates significant advantages in experimental results but also introduces the depth awareness module and a learnable feature fusion module, which enhances the understanding of object structures through depth information and fully utilizes features from various pre-trained models, offering new possibilities for the application of deep learning in RGB and depth data fusion technologies. We will also continue to focus on accelerating model inference and optimizing model lightweighting, enabling our model to operate at a faster speed. Full article
(This article belongs to the Special Issue Machine and Deep Learning in Sensing and Imaging)
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<p>The previous work [<a href="#B39-sensors-24-06680" class="html-bibr">39</a>] (<b>a</b>) found it challenging to differentiate between the front and rear wheels of motorcycles, and our method (<b>b</b>) aids in alleviating this issue. Green lines represent correct matches, and red is incorrect.</p>
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<p>An overview of our method pipeline.</p>
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<p>Pipeline of latent depth awareness module.</p>
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<p>Comparison of PCA from the feature map before and after processing through this module. From left to right: original image, PCA of original feature map, deep feature information, and final result.</p>
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<p>Framework of the feature fusion module.</p>
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<p>Qualitative comparison of dog, horse and sheep categories. Green lines represent correct matches, and red is incorrect. (<b>a</b>) Result of CATs++ [<a href="#B58-sensors-24-06680" class="html-bibr">58</a>], (<b>b</b>) result of DHF [<a href="#B38-sensors-24-06680" class="html-bibr">38</a>], (<b>c</b>) result of SD+DINO [<a href="#B39-sensors-24-06680" class="html-bibr">39</a>], (<b>d</b>) our result. Green lines represent correct matches, and red is incorrect.</p>
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<p>Qualitative comparison of bus, car, and train categories. Green lines represent correct matches, and red is incorrect. (<b>a</b>) Result of CATs++ [<a href="#B58-sensors-24-06680" class="html-bibr">58</a>], (<b>b</b>) result of DHF [<a href="#B38-sensors-24-06680" class="html-bibr">38</a>], (<b>c</b>) result of SD+DINO [<a href="#B39-sensors-24-06680" class="html-bibr">39</a>], (<b>d</b>) our result. Green lines represent correct matches, and red is incorrect.</p>
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<p>Qualitative comparison of person and TV categories. Green lines represent correct matches, and red is incorrect. (<b>a</b>) Result of CATs++ [<a href="#B58-sensors-24-06680" class="html-bibr">58</a>], (<b>b</b>) result of DHF [<a href="#B38-sensors-24-06680" class="html-bibr">38</a>], (<b>c</b>) result of SD+DINO [<a href="#B39-sensors-24-06680" class="html-bibr">39</a>], (<b>d</b>) our result. Green lines represent correct matches, and red is incorrect.</p>
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<p>The limitation of scale differences.</p>
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24 pages, 2623 KiB  
Article
Exploring Recovery Exercises to Enhance Construction Workers’ Willingness for Career Continuity under the Dual-Process Theory: A Perspective from Physical Exercise
by Zimo Zhao, Zhengke Xu, Jia Zhang and Sijie Tan
Buildings 2024, 14(10), 3287; https://doi.org/10.3390/buildings14103287 (registering DOI) - 17 Oct 2024
Abstract
Globally, the construction industry is facing a severe labor shortage, and attracting and retaining workers has become a pressing challenge. This study examined the effect of rehabilitation exercise on construction workers’ willingness to sustain their careers through a questionnaire survey of 479 construction [...] Read more.
Globally, the construction industry is facing a severe labor shortage, and attracting and retaining workers has become a pressing challenge. This study examined the effect of rehabilitation exercise on construction workers’ willingness to sustain their careers through a questionnaire survey of 479 construction workers using a quantitative research methodology. It aims to reveal how the self-efficacy, perceived usefulness, and self-regulation of rehabilitation exercise affect construction workers’ occupational sustainability through physical and psychological recovery. The results of the study show that (1) the rehabilitation exercise self-efficacy and perceived usefulness of recovery exercise positively affect construction workers’ career sustainability intentions and (2) psychological recovery and physical recovery play parallel mediating roles in the effects of rehabilitation exercise self-efficacy, the perceived usefulness of recovery exercise, and self-regulation of recovery exercise on construction workers’ career sustainability intentions. The findings suggest that improving the physical and psychological capital of construction workers through recovery exercise can effectively enhance their career commitment and willingness to be sustainable. This study provides a reference for the design of more comprehensive and systematic rehabilitation and health management programs in the future and offers suggestions from the perspective of recovery exercise for the development of sustainable construction workers. Full article
(This article belongs to the Topic Building a Sustainable Construction Workforce)
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<p>Proposed model diagram for structural equation modeling.</p>
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<p>Exploratory factor analysis result chart.</p>
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<p>Modified structural equation result diagram. Note: *** <span class="html-italic">p</span> &lt; 0.001.</p>
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19 pages, 7269 KiB  
Article
A Group-Enriched Viscoelastic Model for High-Damping Vitrimers with Many Dangling Chains
by Yan Li, Haibo Feng, Jing Xiong and Li Li
Materials 2024, 17(20), 5062; https://doi.org/10.3390/ma17205062 (registering DOI) - 17 Oct 2024
Abstract
Classical viscoelastic models usually only consider the motion of chain segments and the motion of the entire molecular chain; therefore, they will cause inevitable errors when modeling self-healing vitrimer materials with many group movements. In this paper, a group-enriched viscoelastic model is proposed [...] Read more.
Classical viscoelastic models usually only consider the motion of chain segments and the motion of the entire molecular chain; therefore, they will cause inevitable errors when modeling self-healing vitrimer materials with many group movements. In this paper, a group-enriched viscoelastic model is proposed for self-healing vitrimers where the group effect cannot be neglected. We synthesize a specific damping vitrimer with many dangling chains, surpassing the limited loss modulus of conventional engineering materials. Due to the dangling chains, the damping capability can be improved and the group effect cannot be neglected in the synthesized damping vitrimer. The group-enriched viscoelastic model accurately captures the experimental damping behavior of the synthesized damping vitrimer. Our results indicate that the group-enriched viscoelastic model can improve the accuracy of classical viscoelastic models. It is shown that the group effect can be ignored at low frequencies since the chain segments have sufficient time for extensive realignment; however, the group effect can become significant in the case of high frequency or low temperature. Full article
(This article belongs to the Special Issue Mechanical Behavior of Advanced Engineering Materials (2nd Edition))
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<p>(<b>a</b>) Group-enriched model incorporating chain relaxation and group effects. (<b>b</b>) Temperature dependence of relaxation time of chain relaxation. (<b>c</b>) Specific volume–temperature curves of amorphous polymers.</p>
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<p>Schematic Diagram of (<b>a</b>) the generalized Maxwell model and (<b>b</b>) Kelvin–Voigt model.</p>
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<p>(<b>a</b>) Curing process of materials. (<b>b</b>) Synthesis and chemical structure of vitrimer of disulfide bonds.</p>
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<p>FT-IR spectra of APD, BADGE, and the vitrimer of disulfide bonds.</p>
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<p>Temperature sweep results for DMA, including (<b>a</b>) storage modulus and (<b>b</b>) loss modulus.</p>
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<p>Stiffness–damping distribution of engineering and biomaterials [<a href="#B60-materials-17-05062" class="html-bibr">60</a>,<a href="#B61-materials-17-05062" class="html-bibr">61</a>].</p>
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<p>The process of obtaining master curves and parameter identification. (<b>a</b>) Storage modulus at different temperatures as a function of frequency. (<b>b</b>) Horizontal shift factor and fitting results for the viscoelastic part at each temperature. (<b>c</b>) Storage modulus master curve fitting results. (<b>d</b>) Loss modulus master curve fitting results.</p>
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<p>The loss modulus of the group effect. (<b>a</b>) A schematic diagram of the covalent adaptable network. (<b>b</b>) The calculated loss modulus of the group effect as a function of temperature. (<b>c</b>) Groups in the unit structure.</p>
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<p>A comparison of the model with the experiment. (<b>a</b>) The loss modulus of the model without considering group effects. (<b>b</b>) Comparison of models with and without group effects. (<b>c</b>) A comparison of the storage modulus of the group-enriched model with the experiment.</p>
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<p>A comparison of the group-enriched model of the other three samples with experiments of 10 Hz: (<b>a</b>) Sample No. 1; (<b>b</b>) Sample No. 2; (<b>c</b>) Sample No. 3.</p>
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21 pages, 2111 KiB  
Review
Molecular Research Progress on Gametophytic Self-Incompatibility in Rosaceae Species
by Daouda Coulibaly, Feng Gao, Yang Bai, Kenneth Omondi Ouma, Augustine Antwi-Boasiako, Pengyu Zhou, Shahid Iqbal, Amadou Apho Bah, Xiao Huang, Sabaké Tianégué Diarra, Silas Segbo, Faisal Hayat and Zhihong Gao
Horticulturae 2024, 10(10), 1101; https://doi.org/10.3390/horticulturae10101101 (registering DOI) - 17 Oct 2024
Abstract
Self-incompatibility (SI) is a complex mechanism that prevents plants from self-fertilizing to preserve and promote genetic variability. The angiosperm species have developed two different SI systems, the sporophytic (SSI) and the gametophytic (GSI) systems. SI is a significant impediment to steady fruit production [...] Read more.
Self-incompatibility (SI) is a complex mechanism that prevents plants from self-fertilizing to preserve and promote genetic variability. The angiosperm species have developed two different SI systems, the sporophytic (SSI) and the gametophytic (GSI) systems. SI is a significant impediment to steady fruit production in fruit tree species of the Rosaceae. In Rosaceae, GSI is genetically regulated via a single locus, named the ‘S-locus’, which includes a minimum of two polymorphic and relatively intercorrelated S genes: a pistil-expressed S-RNase gene and several pollen-expressed SFBB (S-locus F-Box Brothers) or SFB (S haplotype-specific F-box protein). This necessitates the interaction of S-RNases with the male determinants. Although genetic and molecular analyses of S genes have shown that mutations in both pistils and pollen-specific components induce self-compatibility in many species and cultivars, other genes or molecules outside the S-locus can co-participate in the male gamete rejection in GSI. However, we highlight and synthesize the most recent knowledge on different mechanisms of GSI in Rosaceae in this current review. Full article
(This article belongs to the Special Issue Advances in Fruit Quality and Genetic Improvement)
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<p>Genetic illustration of gametophytic and sporophytic SI.</p>
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<p>Interaction between <span class="html-italic">S-RNase</span> and different sections of <span class="html-italic">SFB</span> in SC <span class="html-italic">Prunus</span> species. (<b>A</b>) <span class="html-italic">S-RNase</span> and <span class="html-italic">SFB</span> F-box motif, (<b>B</b>) <span class="html-italic">S-RNase</span> and <span class="html-italic">SFB</span> variable region 1 (V1), (<b>C</b>) <span class="html-italic">S-RNase</span> and <span class="html-italic">SFB</span> variable region 2 (V2), (<b>D</b>) <span class="html-italic">S-RNase</span> and <span class="html-italic">SFB</span> hypervariable regions a and b (HV-a-b).</p>
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<p>The molecular basis of self/non-self-discrimination implicates the General Inhibitor (GI) degradation model, which is based on <span class="html-italic">Prunus</span> pollen S biochemical functions. (<b>A</b>) Non-self-<span class="html-italic">S-RNase</span> is supposed to be recognized and inhibited by GI. The inhibition of non-self-<span class="html-italic">S-RNase</span> is supposed to be unaffected by <span class="html-italic">SFB</span>. (<b>B</b>) <span class="html-italic">SFB</span> recognizes the self-<span class="html-italic">S-RNase</span>-GI complex and polyubiquitinate GI. Deterioration of the polyubiquitinated GI by the ubiquitin–proteasome system causes the release of active self-<span class="html-italic">S-RNase</span>, enabling incompatibility reaction.</p>
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21 pages, 4478 KiB  
Article
Visual Cues, Liking, and Emotional Responses: What Combination of Factors Result in the Willingness to Eat Vegetables Among Children with Food Neophobia?
by Xiaoqin Tan, Shureen Faris Abdul Shukor and Kim Geok Soh
Foods 2024, 13(20), 3294; https://doi.org/10.3390/foods13203294 (registering DOI) - 17 Oct 2024
Abstract
Childhood nutrition is a cornerstone of long-term health, yet many children exhibit reluctance to consume healthy foods such as vegetables. This aversion can be influenced by various factors, including food neophobia and the sensory and visual appeal of the foods that are being [...] Read more.
Childhood nutrition is a cornerstone of long-term health, yet many children exhibit reluctance to consume healthy foods such as vegetables. This aversion can be influenced by various factors, including food neophobia and the sensory and visual appeal of the foods that are being presented. Hence, understanding how visual cues affect children’s willingness to eat can provide insights into effective strategies to enhance their dietary habits. This research explores the influence of visual cues on the dietary behaviors of children aged 9 to 12, their willingness to consume and request healthy foods such as vegetables, within the context of challenges such as food neophobia. This study examines how intrinsic cues (e.g., vegetable characteristics) and extrinsic cues (e.g., the plate’s color and shape) affect children’s liking and emotional responses, impacting their willingness to eat and request purchases from parents. Conducted using a sample of 420 children, this cross-sectional study reveals that attributes such as a plate’s color and shape significantly affect food-related behaviors and emotions. A validated and reliable self-administered questionnaire was employed. Independent t-tests and ANOVA were used to test the differences between gender and food neophobia, while Spearman correlations were used for correlation analysis. Visual cues served as the independent variables, liking and emotional responses as the mediating variables, and willingness behaviors as the dependent variable. Hierarchical regression analyses were conducted to explore the relationships among intrinsic cues, extrinsic cues, and the mediating effect of liking and emotional responses. Findings show that boys prefer blue and triangular plates, while girls prefer pink plates, generating more positive emotions. Children with food neophobia initially experience aversion, but this can be reduced by enhancing sensory appeal and emotional engagement. The findings underscore the importance of leveraging visual cues and fostering positive emotional experiences to encourage healthier eating habits and increase children’s acceptance and purchase of nutritious foods. Full article
(This article belongs to the Section Sensory and Consumer Sciences)
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<p>The valence× arousal circumplex-inspired emotion word questionnaire (CEQ) used in this research.</p>
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<p>The stimuli of vegetables and plates.</p>
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<p>Visual cues inducing liking of the participants with gender.</p>
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<p>Mean score of willingness behaviors of the participants with gender.</p>
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<p>Visual cues induce Liking of the participants with Food Neophobia.</p>
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<p>Spider plots showing comparison of the “low” FN group, “medium” FN group and “high” FN group for the 12 CEQ emotion word pairs (frequency of use, %) (RQ1). Significant differences are shown with * when <span class="html-italic">p</span> &lt; 0.05, ** <span class="html-italic">p</span> &lt; 0.01 and *** when <span class="html-italic">p</span> &lt; 0.001. The nine visual cues are shown in order.</p>
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<p>Mean score of willingness behaviors of the participants with food neophobia.</p>
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15 pages, 2088 KiB  
Review
The Psychological Nature of Female Gait Attractiveness
by Hiroko Tanabe and Kota Yamamoto
Bioengineering 2024, 11(10), 1037; https://doi.org/10.3390/bioengineering11101037 (registering DOI) - 17 Oct 2024
Abstract
Walking, a basic physical movement of the human body, is a resource for observers in forming interpersonal impressions. We have previously investigated the expression and perception of the attractiveness of female gaits. In this paper, drawing on our previous research, additional analysis, and [...] Read more.
Walking, a basic physical movement of the human body, is a resource for observers in forming interpersonal impressions. We have previously investigated the expression and perception of the attractiveness of female gaits. In this paper, drawing on our previous research, additional analysis, and reviewing previous studies, we seek to deepen our understanding of the function of gait attractiveness. First, we review previous research on gait as nonverbal information. Then, we show that fashion models’ gaits reflect sociocultural genderlessness, while nonmodels express reproductive-related biological attractiveness. Next, we discuss the functions of gait attractiveness based on statistical models that link gait parameters and attractiveness scores. Finally, we focus on observers’ perception of attractiveness, constructing a model of the visual information processing with respect to gait attractiveness. Overall, our results suggest that there are not only biological but also sociocultural criteria for gait attractiveness, and men and women place greater importance on the former and latter criteria, respectively, when assessing female gait attractiveness. This paper forms a major step forward in neuroaesthetics to understand the beauty of the human body and the generation of biological motions. Full article
(This article belongs to the Special Issue Bioengineering of the Motor System)
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<p>Five strategies for women to have an attractive gait.</p>
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<p>A between-group comparison in silhouette-related gait parameters: The values in the first (<b>left</b>) and second (<b>right</b>) rows show a comparison between groups in the normal and attractive-conscious conditions, respectively. The yellow and orange plots represent the model, and the blue and dark blue plots represent the nonmodel. The black bars on the horizontal axis indicate time points showing statistically significant group differences. The horizontal axis shows one stride beginning with a right-heel contact, followed by a left-toe-off (approximately 10.3%), a left-heel contact (approximately 50.7%), and a right-toe-off (approximately 60.3%), ending with a subsequent right-heel contact. Vertical axis: A larger value of each parameter (i.e., first to the fourth rows) represents a further backward head tilt, smaller head rotation in the horizontal plane (that is, facing the front), larger hip twist from the front view, and more backward arm swing, respectively.</p>
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<p>Between-group comparison of energy-related gait parameters: The values for the first (<b>left</b>) and second (<b>right</b>) rows present a comparison between groups in the normal and attractive-conscious conditions, respectively. The yellow and orange lines represent the models, and the blue and dark blue lines represent the nonmodels. The black bars on the horizontal axis present the time points using statistically significant group differences.</p>
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<p>An overview of the walkers’ elements affecting the observers’ attractiveness perception. Models 1 and 2 indicate the evaluation criteria for attractiveness (silhouette-related and health–physique-related standards, respectively) in the two models that were constructed in our previous study [<a href="#B40-bioengineering-11-01037" class="html-bibr">40</a>]. The positive and negative signs indicate whether larger or smaller values lead to higher attractiveness ratings.</p>
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<p>Visual information processing for the attractiveness perception and evaluation of female gaits. The arrows represent the flow of visual processing.</p>
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17 pages, 4709 KiB  
Article
Top-Oil Temperature Prediction of Power Transformer Based on Long Short-Term Memory Neural Network with Self-Attention Mechanism Optimized by Improved Whale Optimization Algorithm
by Dexu Zou, He Xu, Hao Quan, Jianhua Yin, Qingjun Peng, Shan Wang, Weiju Dai and Zhihu Hong
Symmetry 2024, 16(10), 1382; https://doi.org/10.3390/sym16101382 (registering DOI) - 17 Oct 2024
Abstract
The operational stability of the power transformer is essential for maintaining the symmetry, balance, and security of power systems. Once the power transformer fails, it will lead to heightened instability within grid operations. Accurate prediction of oil temperature is crucial for efficient transformer [...] Read more.
The operational stability of the power transformer is essential for maintaining the symmetry, balance, and security of power systems. Once the power transformer fails, it will lead to heightened instability within grid operations. Accurate prediction of oil temperature is crucial for efficient transformer operation. To address challenges such as the difficulty in selecting model hyperparameters and incomplete consideration of temporal information in transformer oil temperature prediction, a novel model is constructed based on the improved whale optimization algorithm (IWOA) and long short-term memory (LSTM) neural network with self-attention (SA) mechanism. To incorporate holistic and local information, the SA is integrated with the LSTM model. Furthermore, the IWOA is employed in the optimization of the hyper-parameters for the LSTM-SA model. The standard IWOA is improved by incorporating adaptive parameters, thresholds, and a Latin hypercube sampling initialization strategy. The proposed method was applied and tested using real operational data from two transformers within a practical power grid. The results of the single-step prediction experiments demonstrate that the proposed method significantly improves the accuracy of oil temperature prediction for power transformers, with enhancements ranging from 1.06% to 18.85% compared to benchmark models. Additionally, the proposed model performs effectively across various prediction steps, consistently outperforming benchmark models. Full article
(This article belongs to the Special Issue Symmetry/Asymmetry Studies in Modern Power Systems)
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<p>The basic construction of an oil-immersed transformer.</p>
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<p>Flow chart of IWOA-LSTM-SA.</p>
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<p>LSTM structure diagram.</p>
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<p>LSTM-SA structure.</p>
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<p>Flow chart of the IWOA.</p>
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<p>Average convergence curves for each algorithm.</p>
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<p>Average convergence curves for each algorithm.</p>
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<p>The prediction results of IWOA-LSTM-SA.</p>
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<p>Training and testing errors over iterations.</p>
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<p>Performance comparison across models.</p>
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<p>Model residuals.</p>
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<p>Multi-step prediction performance comparison across models (one week).</p>
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34 pages, 1600 KiB  
Article
Empowering Women in Tech Innovation and Entrepreneurship: A Qualitative Approach
by Teresa Felgueira, Teresa Paiva, Catarina Alves and Natália Gomes
Educ. Sci. 2024, 14(10), 1127; https://doi.org/10.3390/educsci14101127 (registering DOI) - 17 Oct 2024
Abstract
Women’s participation and leadership in technology and entrepreneurship are crucial for advancing innovation and ensuring inclusive economic development. Despite progress in gender equality, women in tech face challenges like under-representation and systemic biases. This research delves into the multifaceted experiences of women in [...] Read more.
Women’s participation and leadership in technology and entrepreneurship are crucial for advancing innovation and ensuring inclusive economic development. Despite progress in gender equality, women in tech face challenges like under-representation and systemic biases. This research delves into the multifaceted experiences of women in tech innovation and entrepreneurship, highlighting the barriers they face and the strategies they use to overcome them. This study used a qualitative, observational design with semi-structured interviews and thematic analysis to explore the experiences and perceptions of entrepreneurial women in the Science, Technology, Engineering, and Mathematics (STEM) field. It was possible to conclude that entrepreneurial women exhibit a strong sense of autonomy, competence, and leadership, enabling them to manage their ventures independently and effectively. Also, the success of entrepreneurial women is multifaceted, combining personal fulfilment, professional achievements, and financial stability, with positive feedback and recognition playing a key role. Building strong social and supportive relationships is vital for entrepreneurial women, providing a sense of belonging, community, and emotional support. In this context, to empower women in STEM and entrepreneurship, Higher Education Institutions (HEIs) should promote entrepreneurship as a career path, provide practical and value-based education, and foster a work environment of trust, communication, and continuous learning. Full article
(This article belongs to the Special Issue Gender and STEM Education)
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<p>Self-Determination Theory: main objective questions.</p>
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<p>Categories and subcategories of the autonomy construct.</p>
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<p>Categories and subcategories of the competence construct.</p>
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<p>Categories and subcategories of the relatedness construct.</p>
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<p>HEI environment characteristics.</p>
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22 pages, 459 KiB  
Article
Pro-Environmental Behavior of Tourists in Ecotourism Scenic Spots: The Promoting Role of Tourist Experience Quality in Place Attachment
by Jiantao Zhang, Li Jin, Xinning Pan and Yang Wang
Sustainability 2024, 16(20), 8984; https://doi.org/10.3390/su16208984 (registering DOI) - 17 Oct 2024
Abstract
The pro-environmental behavior of tourists is an important guarantee for the sustainable development of ecotourism scenic spots. This article is based on 806 tourist survey data from two ecotourism scenic spots, making use of exploratory factor analysis (EFA) and the structural equation model [...] Read more.
The pro-environmental behavior of tourists is an important guarantee for the sustainable development of ecotourism scenic spots. This article is based on 806 tourist survey data from two ecotourism scenic spots, making use of exploratory factor analysis (EFA) and the structural equation model (SEM) to explore the impact of tourist experience quality on tourists’ pro-environmental behavior. The research findings indicate that (1) the quality of the tourist experience is an important factor affecting the formation of place attachment; (2) the quality of tourism experience directly and positively affects place dependence, and indirectly affects place identity through the mediating factor of place dependence; and (3) the direct impact of tourist experience quality on tourists’ pro-environmental behavior is significant, with place attachment playing a mediating role. This article provides inspiration for scenic spots to encourage tourists to engage in pro-environmental behavior. In the process of stimulating tourists to engage in pro-environmental behaviors, ecotourism scenic spots should pay more attention to the tourist experiences and emotional experiences. Scenic spots should make more efforts to improve the quality of the tourist experience, so that tourists can develop a sense of dependence on ecotourism scenic spots, gradually form self-identification with the scenic spots, and engage in environmentally friendly behavior, helping to achieve the sustainable development of ecotourism scenic spots. Full article
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<p>Hypothesis model.</p>
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15 pages, 3915 KiB  
Article
The pH and Sucrose Influence Rhamnolipid Action Toward Planktonic and Biofilms of Listeria monocytogenes
by Tathiane Ferroni Passos and Marcia Nitschke
Microorganisms 2024, 12(10), 2078; https://doi.org/10.3390/microorganisms12102078 (registering DOI) - 17 Oct 2024
Abstract
Bacterial resistance and persistence in food environments are major concerns for the industry, which constantly seeks new strategies to reduce microbial contamination. Rhamnolipids (RL) biosurfactants are considered sustainable and green alternatives to synthetics; furthermore, they have demonstrated potential for controlling various foodborne pathogens. [...] Read more.
Bacterial resistance and persistence in food environments are major concerns for the industry, which constantly seeks new strategies to reduce microbial contamination. Rhamnolipids (RL) biosurfactants are considered sustainable and green alternatives to synthetics; furthermore, they have demonstrated potential for controlling various foodborne pathogens. Food environments are typically exposed to diverse pH, solutes, temperatures, and water activity (aw) levels that may favor the survival of pathogens. Therefore, it is crucial to consider these factors in evaluating the performance of novel antimicrobials. Our study examined the influence of pH and sucrose on the antimicrobial activity of RL against both planktonic and biofilm of Listeria monocytogenes. We found that the presence of sucrose can enhance the antimicrobial effectiveness of RL against both planktonic and sessile bacteria. The addition of sugar particularly improved RL action at pH 6 and 7. Moreover, we observed that the type and size of RL self-assembly structures depend on the pH and sucrose concentration. These findings suggest potential for developing RL-based innovative methods to control L. monocytogenes in sugar-rich or -low aw foods and environments. Full article
(This article belongs to the Special Issue Advances in Microbial Surfactants: Production and Applications)
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<p>Growth of <span class="html-italic">L. monocytogenes</span> in the presence of 650 mg/L of RL with and without sucrose at (<b>A</b>) pH 5.0, (<b>B</b>) pH 6.0, (<b>C</b>) pH 7.0, (<b>D</b>) pH 8.0, and (<b>E</b>) RL at different pH values and (<b>F</b>) RL + 50% sucrose at different pH values.</p>
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<p>Effect of pH in the viability of <span class="html-italic">L. monocytogenes</span> biofilms treated with 650 mg/L of RL in the presence and absence of 50% sucrose. (<b>A</b>) pH 5, (<b>B</b>) pH 6, (<b>C</b>) pH 7, and (<b>D</b>) pH 8.</p>
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<p>Fluorescence microscopy of the micellar structures of rhamnolipids formed in aqueous solution. (<b>A</b>) pH 5.0, (<b>B</b>) pH 5.0 + 5% sucrose, (<b>C</b>) pH 6.0, (<b>D</b>) pH 6.0 + 5% sucrose, (<b>E</b>) pH 7.0 (<b>F</b>) pH 7.0 + 5% sucrose, (<b>G</b>) pH 8.0, and (<b>H</b>) pH 8.0 + 5% sucrose. Scale bars correspond to 20 μm.</p>
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<p>Cryo-TEM images of RL self-assembly structures at pH 5 in the absence (<b>A</b>), presence of 5% sucrose (<b>B</b>); pH 7 in the absence (<b>C</b>), presence of 5% sucrose (<b>D</b>). The red arrows highlight the predominant structures.</p>
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23 pages, 3427 KiB  
Article
Enhanced Interfaces for High-Temperature Purposes—Practical Methodology and Characterization
by José Pereira, Reinaldo Souza, António Moreira and Ana Moita
Processes 2024, 12(10), 2265; https://doi.org/10.3390/pr12102265 (registering DOI) - 17 Oct 2024
Abstract
The current study is focused on devising treated diatomite interfaces with the robustness and boiling water resistance necessary for high-temperature purposes. This work describes the synthesis methodology of the diatomite-based coatings, which followed the production of a composite formulation composed by treated diatomite [...] Read more.
The current study is focused on devising treated diatomite interfaces with the robustness and boiling water resistance necessary for high-temperature purposes. This work describes the synthesis methodology of the diatomite-based coatings, which followed the production of a composite formulation composed by treated diatomite powder dispersed in an epoxy resin matrix. After its preparation, the suspension was applied via the dip-coating technique over AISI-304 stainless-steel foils, which, after being air dried, underwent a post-curing treatment. Also, the interfaces were characterized by diverse techniques such as scanning electron microscopy and optical tensiometry. Apart from this, their thermophysical properties like thermal conductivity were also determined. Further, the physical and chemical durability of the interfaces was also evaluated via the elaboration of robustness tests including abrasion resistance, adhesion strength, solid impact resistance, and solvent resistance. The results showed satisfactory resistant interfaces, and with a wettability characterized by contact angles superior to 150°. Also, the interfaces confirmed improved durability when immersed in boiling water at 1 atm, since their wetting characteristics and durability remained nearly unaltered after 762 h of testing. Additionally, the synthesized interfaces possessed self-cleaning ability and chemical and thermal shock aging resistance. Generally, the fundamental outcomes of this work point out the suitability of the produced diatomite-based interfaces to be explored in high-temperature applications like flow boiling, pool boiling, and condensation. In terms of practicality, the method of preparation of the interfaces was a relatively easy and rapid approach to obtaining enhanced wettability and resilient interfaces, and with the required adaptations like the ratios between the raw materials, its suitability for large-scale applications makes this an appealing option. Full article
(This article belongs to the Special Issue Interfacial Structure-Mediated Controllable Adhesion and Assembly)
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<p>Substrate and coatings: (<b>a</b>) substrate made of stainless-steel AISI 304, (<b>b</b>) the same substrate after etching, (<b>c</b>) coating with a particle loading of 25%, (<b>d</b>) coating with a particle loading of 35%, and (<b>e</b>) coating with a particle loading of 45%.</p>
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<p>Fourier transform infrared spectroscopy spectra of the untreated diatomite powder (red line) and diatomite treated with PFOTS (orange line).</p>
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<p>Changes in the water contact angle as a function pool boiling duration.</p>
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<p>Contact angles for the coatings and substrate: (<b>a</b>) PL = 25% coating, (<b>b</b>) PL = 35% coating, (<b>c</b>) PL = 45% coating, and (<b>d</b>) stainless-steel substrate.</p>
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<p>Obtained SEM micrographs: (<b>a</b>) as-received diatomite powder showing the general view of the microstructure with disk-shaped particles reaching more than 60 µm of diameter, (<b>b</b>) details of the enhanced disk-shaped particle of the fluorinated diatomite powder, (<b>c</b>) structure overview of the diatomite–PFOTS–epoxy coating with 25% particle loading, (<b>d</b>) diatomite disk-shaped particle with approximately 60 µm of diameter in the 25% particle loading coating, (<b>e</b>) disk-shaped particles of the 35% particle loading coating, and (<b>f</b>) highly porous structure of the particles in the 45% particle loading coating.</p>
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<p>SEM micrographs highlighting the increased porosity of the developed coatings and the hierarchical porous structure of the diatomite particles: (<b>a</b>) unmodified diatomite, (<b>b</b>,<b>c</b>) fluorinated diatomite.</p>
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<p>Change in the water contact angle as a function of the number of peeling cycles during the adhesion strength test.</p>
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<p>Change in the water contact angle over the duration of the solid impact resistance test.</p>
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<p>Changes in the water contact angle over the duration of the suspension erosion resistance test.</p>
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<p>Evolution of the water contact angle against the number of cycles of the thermal shock aging resistance test.</p>
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<p>Evolution of the water contact angle against the number of days of the chemical aging resistance test.</p>
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<p>Evolution of the water contact angle against the duration of the acidic corrosion resistance test.</p>
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<p>Evolution of the water contact angle against the duration of the alkaline corrosion resistance test.</p>
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<p>Self-cleaning test for the PL = 25% coating: (<b>a</b>) coating entirely covered with aluminum oxide grains, (<b>b</b>) after dropping a few drops of dyed distilled water, and (<b>c</b>) coating entirely cleaned in all its extension.</p>
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18 pages, 4036 KiB  
Article
Theoretical Research and Numerical Analysis of a New Assembled Shuttle-Shaped Self-Centering Mild Steel Energy Dissipation Brace
by Yao Chen, Zhonghua Liu and Jianchao Zhao
Buildings 2024, 14(10), 3285; https://doi.org/10.3390/buildings14103285 (registering DOI) - 17 Oct 2024
Abstract
To solve the problem of large residual deformation and high repair cost of traditional frame structures after an earthquake, a new type of assembled shuttle-shaped self-centering mild steel energy dissipation brace (ASSSEDB) with stable stiffness, material saving, and easy replacement was proposed. The [...] Read more.
To solve the problem of large residual deformation and high repair cost of traditional frame structures after an earthquake, a new type of assembled shuttle-shaped self-centering mild steel energy dissipation brace (ASSSEDB) with stable stiffness, material saving, and easy replacement was proposed. The plastic deformation of mild steel is used to dissipate energy, and the disc spring system provides a reset function. Based on the working mechanism of energy dissipation brace, a restoring force model for the ASSSEDB was established, and then the numerical analysis was carried out by ANSYS to verify the accuracy of the proposed model. The results confirm that the ASSSEDB has stable energy dissipation ability and a resetting function, with a full hysteresis curve. The finite element analysis results align well with the developed restoring force model, and the maximum deviations of initial stiffness and ultimate capacity are, respectively, 1.4% and 2.3%, which indicates that the established restoring force model can provide a theoretical basis for design of the ASSSEDB. Furthermore, the time history analysis was carried out to assess the seismic performance of a six-story steel frame structure using the proposed ASSSEDB. The results show that compared with the steel frame structure with BRBs, the proposed ASSSEDB can decrease the residual deformation of structures by up to 93.41%. The self-centering ratio of the ASSSEDB is crucial in controlling residual deformation of structures, and it is recommended to be greater than 1.0. Full article
(This article belongs to the Section Building Structures)
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<p>Structural details of the ASSSEDB. 1. guide rod; 2. pre-tightening nut; 3. disc spring; 4. shuttle-shaped tube; 5. mild steel energy dissipation element; 6. energy dissipation fixing piece; 7. cavity body; 8. ear plate; 9. middle enclosed cavity; 10. fixed baffle; 11. end cavity.</p>
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<p>Bilinear model of the mild steel energy dissipation element system.</p>
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<p>Restoring force model of disc spring system.</p>
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<p>Restoring force model of the ASSSEDB.</p>
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<p>Loading setup of experiment in Amadeo et al. [<a href="#B36-buildings-14-03285" class="html-bibr">36</a>].</p>
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<p>Comparison of Amadeo et al.’s [<a href="#B36-buildings-14-03285" class="html-bibr">36</a>] experimental results and numerical analysis results.</p>
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<p>Finite element model of ASSSEDB.</p>
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<p>Comparison of finite element model results with restoring force model results.</p>
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<p>Plan and elevation of braced steel frame structure. (<b>a</b>) Structural plan (unit: mm); (<b>b</b>) Structural elevation (unit: mm).</p>
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<p>Plan and elevation of braced steel frame structure. (<b>a</b>) Structural plan (unit: mm); (<b>b</b>) Structural elevation (unit: mm).</p>
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<p>Numerical model of braced steel frame structure.</p>
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<p>Story drift of structures. (<b>a</b>) 70 gal; (<b>b</b>) 200 gal; (<b>c</b>) 400 gal; (<b>d</b>) BRB; (<b>e</b>) FAR.</p>
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<p>Residual deformation of structures. (<b>a</b>) 70 gal; (<b>b</b>) 200 gal; (<b>c</b>) 400 gal; (<b>d</b>) BRB.</p>
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<p>Initial stiffness of ASSSEDB. (<b>a</b>) Maximum displacement; (<b>b</b>) Story drift; (<b>c</b>) Residual deformation.</p>
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<p>Self-centering ratio of ASSSEDB. (<b>a</b>) Maximum displacement; (<b>b</b>) Story drift; (<b>c</b>) Residual deformation.</p>
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18 pages, 10072 KiB  
Article
Exploring the Impact of School-Based Physical and Perceived Environments on Students’ Physical Activity During Recess: A Case Study of Four Schools in Xi’an, China
by Qing Wang, Yan Wang, Lan Zhou and Yong Nan
Buildings 2024, 14(10), 3283; https://doi.org/10.3390/buildings14103283 (registering DOI) - 17 Oct 2024
Viewed by 71
Abstract
In the context of high academic pressure and inadequate physical activity (PA) among adolescents, it is important to study how the physical environment and students’ perceptions affect their physical activity during school recess. An empirical study was conducted in four secondary schools in [...] Read more.
In the context of high academic pressure and inadequate physical activity (PA) among adolescents, it is important to study how the physical environment and students’ perceptions affect their physical activity during school recess. An empirical study was conducted in four secondary schools in Xi’an to gather data on students’ physical activity using DJI Mini Drone recordings and self-reported questionnaires. Three physical activity indices—concentration, type richness and intensity—were visualized and quantified for intercomparison. This study found that physical environmental factors such as the building shape complexity and green space proportion significantly affected the PA concentration and intensity indices, whereas the campus area per student, the average distance to PA facilities and the number of PA facilities indicated a strong correlation with the PA richness index at the school level. Additionally, perceived environmental factors like space safety, facilities enjoyment and visual accessibility exerted influence on the PA intensity and frequency at the individual level. The study’s results confirmed previous findings from a social–ecological perspective and provide a comprehensive assessment framework that includes the spatial organization/spatial quality of the school and the perceived variables of safety, comfort, aesthetics, accessibility and functionality. By incorporating spatial, perceptual and behavioral aspects, this approach provides an efficient and integrated analytical methodology to promote adolescents’ health in school. Full article
(This article belongs to the Section Architectural Design, Urban Science, and Real Estate)
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<p>Methodical framework of this study.</p>
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<p>Urban location, community and surroundings, site plan and DJI Mini Drone view of the PA records of the four sample schools.</p>
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<p>Classification for outside public space as observing PA areas.</p>
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<p>Sankey map of students’ physical activity distribution for different sexes, intensity levels and types in the four sample schools.</p>
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<p>Grid division, coding and physical activity placement of outdoor public space on a sample campus (taking the WS school as an example).</p>
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<p>Spatial distribution (grids) of PA in three categories in each sample school.</p>
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<p>Comparison of PA indices in three categories in each sample school.</p>
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<p>Matrix diagram of the correlation between physical environmental factors and PA student numbers in different public spaces.</p>
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<p>Matrix diagram of the correlation between physical environmental factors and PA indices in three categories.</p>
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<p>Line chart of the mean value and standard deviation of respondents’ evaluations on the perceived environmental factors.</p>
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<p>Dot plot of the correlation between the perceived environmental factors and PA intensity/frequency.</p>
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15 pages, 299 KiB  
Article
A Mixed Methods Study of Ethnic Identity and Mental Health Recovery Processes in Minoritized Young Adults
by Kiara L. Moore, Aaron H. Rodwin, Rei Shimizu and Michelle R. Munson
Healthcare 2024, 12(20), 2063; https://doi.org/10.3390/healthcare12202063 (registering DOI) - 17 Oct 2024
Viewed by 65
Abstract
Background/Objectives: Ethnic identity development is associated with positive mental health in young adults from ethnic minority groups. How a sense of belonging and attachment to one’s ethnic culture is related to personal mental health recovery remains unexplained. This study examines the experiences of [...] Read more.
Background/Objectives: Ethnic identity development is associated with positive mental health in young adults from ethnic minority groups. How a sense of belonging and attachment to one’s ethnic culture is related to personal mental health recovery remains unexplained. This study examines the experiences of ethnic minority young adults in the U.S. to understand the aspects of culture and identity development that are relevant to their recovery processes. Methods: Young adults who were living with chronic mental disorders were recruited from four rehabilitation programs. Interviews produced quantitative and qualitative data. An explanatory sequential mixed methods design was used to integrate the qualitative findings from a sub-group of young adults (n = 44) with the results from the quantitative study. Directed content analysis was used to analyze the qualitative data, and the integrated data were analyzed in joint displays. Results: The prominent themes characterizing ethnic identity development in personal recovery were (a) cultural history, traditions, and values; (b) mental illness stigma within the ethnic community; and (c) bias and discrimination in mental health services. Young adults with high ethnic identity development reported having more support from family, but they also described experiences with stigma and racism. Conclusions: The integrated results suggest that ethnic identity development promotes mental health recovery in minoritized young adults through social support and improved well-being and resilience. Experiences of intersectional stigma and structural racism associated with ethnic identity can interfere with self-determination and access to care among minoritized Hispanic/Latine, Black, and multiracial young adults in the U.S. Full article
(This article belongs to the Special Issue Mental Health at a Personal, Communal, and Social Level)
13 pages, 1968 KiB  
Article
Gintonin Stimulates Glucose Uptake in Myocytes: Involvement of Calcium and Extracellular Signal-Regulated Kinase Signaling
by Rami Lee, Kyung-Jong Won, Ji-Hun Kim, Byung-Hwan Lee, Sung-Hee Hwang and Seung-Yeol Nah
Biomolecules 2024, 14(10), 1316; https://doi.org/10.3390/biom14101316 (registering DOI) - 17 Oct 2024
Viewed by 83
Abstract
Ginseng has anti-hyperglycemic effects. Gintonin, a glycolipoprotein derived from ginseng, also stimulates insulin release from pancreatic beta cells. However, the role of gintonin in glucose metabolism within skeletal muscle is unknown. Here, we showed the effect of gintonin on glucose uptake, glycogen content, [...] Read more.
Ginseng has anti-hyperglycemic effects. Gintonin, a glycolipoprotein derived from ginseng, also stimulates insulin release from pancreatic beta cells. However, the role of gintonin in glucose metabolism within skeletal muscle is unknown. Here, we showed the effect of gintonin on glucose uptake, glycogen content, glucose transporter (GLUT) 4 expression, and adenosine triphosphate (ATP) content in C2C12 myotubes. Gintonin (3–30 μg/mL) dose-dependently stimulated glucose uptake in myotubes. The expression of GLUT4 on the cell membrane was increased by gintonin treatment. Treatment with 1–3 μg/mL of gintonin increased glycogen content in myotubes, but the content was decreased at 30 μg/mL of gintonin. The ATP content in myotubes increased following treatment with 10–100 μg/mL gintonin. Gintonin transiently elevated intracellular calcium concentrations and increased the phosphorylation of extracellular signal-regulated kinase (ERK). Gintonin-induced transient calcium increases were inhibited by treatment with the lysophosphatidic acid receptor inhibitor Ki16425, the phospholipase C inhibitor U73122, and the inositol 1,4,5-trisphosphate receptor antagonist 2-aminoethoxydiphenyl borate. Gintonin-stimulated glucose uptake was decreased by treatment with U73122, the intracellular calcium chelator 1,2-bis(o-aminophenoxy)ethane-N,N,N′,N′-tetraacetic acid tetra(acetoxymethyl) ester, and the ERK inhibitor PD98059. These results show that gintonin plays a role in glucose metabolism by increasing glucose uptake through transient calcium increases and ERK signaling pathways. Thus, gintonin may be beneficial for glucose metabolism control. Full article
(This article belongs to the Special Issue Therapeutic Potential of Natural Products in Metabolic Diseases)
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<p>Effect of gintonin on the cell viability of myotubes and transient intracellular calcium increases. (<b>A</b>) Cell viability. C2C12 myotubes were treated with gintonin (GT, 0.1–100 μg/mL) or lysophosphatidic acid (10 μM) for 24 h. Then, WST assay was performed. All data are shown as the mean ± SEM (<span class="html-italic">n</span> = 6). (<b>B</b>,<b>C</b>) Transient intracellular calcium increases. Fura-2-AM-incorporated C2C12 myotubes were treated with gintonin (GT, 0.3–30 μg/mL), and intracellular calcium levels were measured by spectrofluorophotometry and calculated. Each arrow in panel (<b>B</b>) represents time points of treatment with gintonin at indicated concentrations. The horizontal length of the upper scale bar corresponds to 100 s (100 s). (<b>D</b>) Inhibitory effects of inhibitors on GT-induced intracellular calcium increase. Fura-2-AM-loaded C2C12 myotubes were pretreated with PTX (100 ng/mL), Ki16425 (10 μM), U73122 (5 μM), or 2-APB (100 μM) for 5 min and then treated with gintonin (GT, 1 μg/mL). All data are shown as the mean ± SEM (<span class="html-italic">n</span> = 3–5); * <span class="html-italic">p</span> &lt; 0.05; ** <span class="html-italic">p</span> &lt; 0.01; *** <span class="html-italic">p</span> &lt; 0.001 vs. untreated control cells (Con). PTX, pertussis toxin; 2-APB, the inositol 1,4,5-trisphosphate receptor antagonist 2-aminoethoxydiphenyl borate.</p>
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<p>Effect of gintonin on glucose uptake in C2C12 myotubes. (<b>A</b>) C2C12 myotubes were treated with gintonin (GT, 3 μg/mL) and 2-NBDG (100 μM) for 0–24 h. (<b>B</b>) The myotubes were treated with gintonin (0.1–30 μg/mL) for 24 h. (<b>C</b>–<b>F</b>) The myotubes were treated with gintonin (GT, 3 μg/mL) and 2-NBDG (100 μM) for 24 h, with or without the addition of inhibitors (Ki16425, 10 μM; U73122, 5 μM; BAPTA-AM, 50 μM; PD98059, 10 μM). Then, 2-NBDG uptake was measured using spectrofluorophotometry. All data are shown as the mean ± SEM. (<span class="html-italic">n</span> = 6); * <span class="html-italic">p</span> &lt; 0.05; ** <span class="html-italic">p</span> &lt; 0.01; *** <span class="html-italic">p</span> &lt; 0.001 vs. time 0 or untreated control cells (Con). <sup>#</sup> <span class="html-italic">p</span> &lt; 0.05; <sup>##</sup> <span class="html-italic">p</span> &lt; 0.01; <sup>###</sup> <span class="html-italic">p</span> &lt; 0.001 vs. GT alone. BAPTA-AM, 1,2-bis(o-aminophenoxy)ethane-N,N,N′,N′-tetraacetic acid tetra(acetoxymethyl) ester.</p>
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<p>Effect of gintonin on ATP and glycogen content in C2C12 myotubes. (<b>A</b>,<b>B</b>) ATP content. (<b>A</b>) C2C12 myotubes were treated with gintonin (GT, 10 μg/mL) for 0–24 h. (<b>B</b>) The myotubes were treated with gintonin (0.1–100 μg/mL) or lysophosphatidic acid (LPA, 10 μM) for 8 h. The ATP content of myotubes was measured using an ATP assay kit. (<b>C</b>) Glycogen content. The myotubes were treated with gintonin (GT, 0.1–30 μg/mL) or insulin (INS, 100 nM), and the glycogen content was measured using a glycogen assay kit. All data are presented as the mean ± SEM (<span class="html-italic">n</span> = 6); * <span class="html-italic">p</span> &lt; 0.05; ** <span class="html-italic">p</span> &lt; 0.01; *** <span class="html-italic">p</span> &lt; 0.001 vs. time 0 or untreated control cells (Con).</p>
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<p>Effect of gintonin on ERK phosphorylation in C2C12 myotubes. (<b>A</b>) C2C12 myotubes were treated with gintonin (GT, 10 μg/mL) for 0–60 min. (<b>B</b>) The myotubes were treated with gintonin (0.3–100 μg/mL) for 10 min. (<b>C</b>) The myotubes were pretreated with inhibitors (Ki16425, 10 μM; PD98059, 10 μM; U73122, 5 μM) for 1 h and then treated with gintonin (3 μg/mL) for 10 min. Phosphorylated ERK and ERK were detected by immunoblotting. All data are shown as the mean ± SEM (n = 4); * <span class="html-italic">p</span> &lt; 0.05; ** <span class="html-italic">p</span> &lt; 0.01; *** <span class="html-italic">p</span> &lt; 0.001 vs. time 0 or untreated control cells (Con). <sup>#</sup> <span class="html-italic">p</span> &lt; 0.05; <sup>##</sup> <span class="html-italic">p</span> &lt; 0.01; <sup>###</sup> <span class="html-italic">p</span> &lt; 0.001 vs. GT alone. p-ERK, phospho-ERK. Original western blot images can be found in <a href="#app1-biomolecules-14-01316" class="html-app">Supplementary File S1</a>.</p>
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<p>Effect of gintonin on GLUT4 expression in total lysates and plasma membrane fractions of C2C12 myotubes. (<b>A</b>) GLUT4 expression in total lysates. (<b>B</b>) GLUT4 expression in the plasma membrane fraction. C2C12 myotubes were treated with gintonin (GT, 10 μg/mL) for 120 min or insulin (INS, 100 nM) for 30 min. GLUT4 expression in total lysates and plasma membrane fraction of C2C12 myotubes was detected by immunoblotting. β-actin and Na<sup>+</sup>/K<sup>+</sup> ATPase were also detected as loading controls. All data are shown as the mean ± SEM (<span class="html-italic">n</span> = 4); ** <span class="html-italic">p</span> &lt; 0.01; *** <span class="html-italic">p</span> &lt; 0.001 vs. untreated control cells (Con). Original western blot images can be found in <a href="#app1-biomolecules-14-01316" class="html-app">Supplementary File S1</a>.</p>
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<p>Possible signaling pathways of gintonin (GT)-induced glucose uptake in C2C12 myotubes. Gintonin induces transient increases in intracellular calcium concentrations and ERK activation via LPA receptor (LPAR) activation. These may lead to increases in the expression and translocation of GLUT4, subsequently increasing glucose uptake. PLC, phospholipase C; ERK, extracellular signal-regulated kinase; GLUT4, glucose transporter type 4.</p>
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