Search Results (3,893)

Mitogen-activated protein kinase (MAPK/MPK) cascades are pivotal and highly conserved signaling modules widely distributed in eukaryotes; they play essential roles in plant growth and development, as well as biotic and abiotic stress responses. With the development of sequencing technology, the complete genome assembly of rice without gaps, T2T (Telomere-to-Telomere)—NIP (version AGIS-1.0), has recently been released. In this study, we used bioinformatic approaches to identify and analyze the rice MPK kinases (MKKs) based on the complete genome. A total of seven OsMKKs were identified, and their physical and chemical properties, chromosome localization, gene structure, subcellular localization, phylogeny, family evolution, and cis-acting elements were evaluated. OsMKKs can be divided into four subgroups based on phylogenetic relationships, and the family members located in the same evolutionary branch have relatively similar gene structures and conserved domains. Quantitative real-time PCR (qRT-PCR) revealed that all OsMKKs were highly expressed in rice seedling leaves. The expression levels of all OsMKKs were more or less altered under exogenous hormone and abiotic stress treatments, with OsMKK1, OsMKK6, and OsMKK3 being induced under almost all treatments, while the expression of OsMKK4 and OsMKK10-2 was repressed under salt and drought treatments and IAA treatment, respectively. In this study, we also summarized the recent progress in rice MPK cascades, highlighted their diverse functions, and outlined the potential MPK signaling network, facilitating further studies on OsMKK genes and rice MPK cascades. Full article

Peanuts grown in saline alkali soil are also subjected to drought stress caused by water scarcity. Therefore, we used HY25 (peanut variety) as an experimental material to investigate the effects of drought on the height of peanut main stems, length of the first lateral branch, leaf area per plant, SPAD value, net photosynthetic rate, and accumulation and distribution of photosynthetic products in saline alkali soil. The results showed that the combined stress of short-term drought and salt significantly reduced the main stem height, first lateral branch length, single plant leaf area, SPAD value, net photosynthetic rate (Pn), intercellular carbon dioxide concentration (Ci), and dry matter accumulation of peanuts, including a decrease in single plant pod yield, 100-pod weight, 100-kernel weight, and peanut yield. And the impact of drought stress on peanut yield varies at different growth stages. For example, under drought stress alone, the sensitive period is the 40th day after planting (40D) > 60th day after planting (60D) > 30th day after planting (30D). Short-term drought has the greatest impact on peanut yield at 40D, while in contrast, resuming watering after drought at 30D results in a slight but not significant increase in peanut yield in comparison with the control. Under the combined stress of drought and salt, the sensitive period of peanuts was 40D > 30D > 60D, and the single pod weight of peanuts was significantly reduced by 15.26% to 57.60% from the flowering stage to the pod stage under drought treatment compared to salt treatment, indicating a significant interaction between drought and salt stress, reducing the single leaf area and net photosynthetic rate of peanut leaves, ultimately leading to a decrease in peanut yield. Therefore, when planting peanuts in saline alkali soil, drought should be avoided, especially early drought, in order to prevent the combined effects of drought and salt stress from harming peanut yield. Full article

Soybean [Glycine max (L.) Merr.], as a globally commercialized crop, is an important source of protein and oil for both humans and livestock. With more frequent extreme weather disasters, abiotic stress has become one of the critical factors restricting soybean production. Proline (Pro) is a well-known substance in plants that responds to abiotic stress. To identify potential effector genes involved in soybean resistance to abiotic stress, we focused on the pyrroline-5-carboxylate dehydrogenase (P5CDH) which is a key enzyme in the degradation process of Pro. Through homologous sequence alignment, phylogenetic tree, and predicted expression, we chose GmP5CDH1 (Glyma.05G029200) for further research. Tissue-specific expression assay showed that GmP5CDH1 had higher expression levels in soybean seed and cotyledon development. Subcellular localization assay revealed that GmP5CDH1 was a nuclear-membrane-localized protein. As the result of the predicted cis-acting regulatory element indicates, the expression level of GmP5CDH1 was induced by low temperature, drought, salt stress, and ABA in soybean. Next, we constructed transgenic Arabidopsis overexpressing GmP5CDH1. The results showed that GmP5CDH1 also strongly responded to exogenous Pro, and overcame the toxicity of abiotic stress on plants by regulating the endogenous concentration of Pro. The interaction between GmP5CDH1 and GmSAM1 was validated through yeast two-hybrid, LUC fluorescence complementary, and BIFC. In conclusion, overexpression of a soybean pyrroline-5-carboxylate dehydrogenase GmP5CDH1 regulates the development of Arabidopsis thaliana by altering proline content dynamically under salt stress, especially improving the growth of plants under exogenous Pro. Full article

This study aimed to explore the role of the trehalose-6-phosphate synthase (TPS) gene family in the adaptation of peas to environmental stress. A comprehensive analysis of the PsTPS gene family identified 20 genes with conserved domains and specific chromosomal locations. Phylogenetic analysis delineated evolutionary relationships, while gene structure analysis revealed compositional insights, and motif analysis provided functional insights. Cis-regulatory element identification predicted gene regulation patterns. Tissue-specific and stress-induced expression profiling highlighted eight genes with ubiquitous expression, with PsTPS15 and PsTPS18 displaying elevated expression levels in roots, nodules, and young stems, and PsTPS13 and PsTPS19 expression downregulated in seeds. Transcriptome analysis identified a differential expression of 20 PsTPS genes, highlighting the significance of 14 genes in response to drought and salinity stress. Notably, under drought conditions, the expression of PsTPS4 and PsTPS6 was initially upregulated and then downregulated, whereas that of PsTPS15 and PsTPS19 was downregulated. Salinity stress notably altered the expression of PsTPS4, PsTPS6, and PsTPS19. Taken together, these findings elucidate the regulatory mechanisms of the PsTPS gene family and their potential as genetic targets for enhancing crop stress tolerance. Full article

Salt stress results in physiological changes that inhibit plant growth and development. Ca-complex sources are used as a potential salt stress attenuator. This study was carried out with the aim of verifying the effects of Ca-complex sources in reducing the effects of saline water stress on the physiological aspects of sour passion fruit seedlings. The experiment was carried out in a randomized block design with a 2 × 2 × 3 factorial scheme, consisting of two cultivars of sour passion fruit (BRS GA1 and BRS SC1), two levels of water salinity (electrical conductivity of 0.5 and 4.0 dS m⁻¹) and three sources of Ca-complex (without, organic acids and amino acids). The traits measured at 60 days after sowing were gas exchange, chlorophyll indices, chlorophyll fluorescence, electrolyte leakage, and relative water content in the leaf limb. Under moderate water salinity, the application of Ca-complex in amino acids promoted increases of 49.84% and 43.71%, respectively, in the efficiency of water use and carboxylation. The application of complex sources increased the stability of cell membranes, reducing electrolyte leakage, providing higher relative water content in seedlings irrigated with moderately saline water. From the results, we conclude that Ca-complex sources have potential as modulators of moderately saline water stress in sour passion fruit seedlings. Full article

Serine/arginine-rich (SR) proteins mostly function as splicing factors for pre-mRNA splicing in spliceosomes and play critical roles in plant development and adaptation to environments. However, detailed study about SR proteins in legume plants is still lacking. In this report, we performed a genome-wide investigation of SR protein genes in wild soybean (Glycine soja) and identified a total of 31 GsSR genes from the wild soybean genome. The analyses of chromosome location and synteny show that the GsSRs are unevenly distributed on 15 chromosomes and are mainly under the purifying selection. The GsSR proteins can be phylogenetically classified into six sub-families and are conserved in evolution. Prediction of protein phosphorylation sites indicates that GsSR proteins are highly phosphorylated proteins. The protein–protein interaction network implies that there exist numerous interactions between GsSR proteins. We experimentally confirmed their physical interactions with the representative SR proteins of spliceosome-associated components such as U1-70K or U2AF35 by yeast two-hybrid assays. In addition, we identified various stress-/hormone-responsive cis-acting elements in the promoter regions of these GsSR genes and verified their expression patterns by RT-qPCR analyses. The results show most GsSR genes are highly expressed in root and stem tissues and are responsive to salt and alkali stresses. Splicing analysis showed that the splicing patterns of GsSRs were in a tissue- and stress-dependent manner. Overall, these results will help us to further investigate the biological functions of leguminous plant SR proteins and shed new light on uncovering the regulatory mechanisms of plant SR proteins in growth, development, and stress responses. Full article

Background: Probiotics, which are live microorganisms that, when given in sufficient quantities, promote the host’s health, have drawn a lot of interest for their ability to enhance gut health. Enterococcus faecalis, a member of the human gut microbiota, has shown promise as a probiotic candidate due to its functional attributes. However, safety concerns associated with certain strains warrant comprehensive evaluation before therapeutic application. Materials and Methods: In this study, E. faecalis EF-2001, originally isolated from fecal samples of a healthy human infant, was subjected to a multi-faceted assessment for its safety and probiotic potential. In silico analysis, CAZyme, biosynthetic, and stress-responsive proteins were identified. Results: The genome lacked biogenic amine genes but contained some essential amino acid and vitamin synthetic genes, and carbohydrate-related enzymes essential for probiotic properties. The negligible difference of 0.03% between the 1^st and 25^th generations indicates that the genetic information of the E. faecalis EF-2001 genome remained stable. The live E. faecalis EF-2001 (E. faecalis EF-2001L) demonstrated low or no virulence potential, minimal D-Lactate production, and susceptibility to most antibiotics except some aminoglycosides. No bile salt deconjugation or biogenic amine production was observed in an in vitro assay. Hemolytic activity assessment showed a β-hemolytic pattern, indicating no red blood cell lysis. Furthermore, the EF-2001L did not produce gelatinase and tolerated simulated gastric and intestinal fluids in an in vitro study. Similarly, heat-killed E. faecalis EF-2001 (E. faecalis EF-2001HK) exhibits tolerance in both acid and base conditions in vitro. Further, no cytotoxicity of postbiotic EF-2001HK was observed in human colorectal adenocarcinoma HT-29 cells. Conclusions: These potential properties suggest that probiotic and postbiotic E. faecalis EF-2001 could be considered safe and retain metabolic activity suitable for human consumption. Full article

Salt stress significantly impairs plant growth, presenting a challenge to agricultural productivity. Exploring the regulatory mechanisms underlying salt stress responses is critically important. Here, we identified a significant role for the maize LESION-SIMULATING DISEASE transcription factor, ZmLSD1, in enhancing salt stress response. Subcellular localization analysis indicated that ZmLSD1-GFP was localized in the nucleus in the maize protoplast. Overexpressing ZmLSD1 in maize obviously enhanced the tolerance of plants to salt stress. Physiological analysis indicated that overexpressed ZmLSD1 in maize could mitigate the accumulation of H₂O₂ and MDA content exposed to salt stress. RNA-seq and qPCR-PCR analyses showed that ZmLSD1 positively regulated ZmWRKY29 expression. ChIP-qPCR and EMSA experiments demonstrated that ZmLSD1 could directly bind to the promoter of ZmWRKY29 through the GTAC motif both in vitro and in vivo. Overall, our findings suggest that ZmLSD1 plays a positive role in enhancing the tolerance of maize to salt by affecting ZmWRKY29 expression. Full article

Rice (Oryza sativa L.), a globally staple food crop, frequently encounters growth, developmental, and yield limitations due to phosphate deficiency. LOW PHOSPHATE ROOT1/2 (LPR1/2) are essential genes in plants that regulate primary root growth and respond to local phosphate deficiency signals under low phosphate stress. In rice, five LPR genes, designated OsLPR1–OsLPR5 based on their sequence identity with AtLPR1, have been identified. OsLPR3 and OsLPR5 are specifically expressed in roots and induced by phosphate deficiency, contributing to rice growth, development, and the maintenance of phosphorus homeostasis under low phosphate stress. In contrast, OsLPR2 is uniquely expressed in shoots, suggesting it may have distinct functions compared with other family members. This study employed Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)-associated protein 9 (CRISPR/Cas9) gene editing technology to generate oslpr2 mutant transgenic lines and subsequently investigated the effect of OsLPR2 gene knockout on rice growth, phosphate utilization, and salt stress tolerance in the seedling stage, as well as the effect of OsLPR2 gene knockout on rice development and agronomic traits in the maturation stage. The results indicated that the knockout of OsLPR2 did not significantly impact rice seedling growth or phosphate utilization, which contrasts significantly with its homologous genes, OsLPR3 and OsLPR5. However, the mutation influenced various agronomic traits at maturity, including plant height, tiller number, and seed setting rate. Moreover, the OsLPR2 mutation conferred enhanced salt stress tolerance in rice. These findings underscore the distinct roles of OsLPR2 compared with other homologous genes, establishing a foundation for further investigation into the function of the OsLPR family and the functional differentiation among its members. Full article

(1) Introduction: Topical dithranol is still commonly used today as an effective treatment for psoriasis. Dithranol treatment is often supplemented with balneotherapy, which has been shown to increase effectiveness and reduce side effects. The inorganic salts (sulfhide, selenium, zinc) are usually thought to be responsible for the effect. The antioxidant effect of the waters is thought to be behind the therapeutic effect, for which inorganic substances (sulfides, selenium, zinc) are thought to be responsible. The organic matter content of medicinal waters is also particularly important, as humic acids, which are often found in medicinal waters, have antioxidant effects. (2) Methods: In this short-term experiment, we aimed to test the possible protective effect of Szigetvár medicinal water and its organic matter isolate on HaCaT cells exposed to dithranol. Malondialdehyde levels were measured, and RT-qPCR was used to investigate the gene expression of selected cytokines relevant in the oxidative stress response (IL-6, IL-8, TNF-α, GM-CSF) and the expression of microRNA-21. (3) Results: Szigetvár medicinal water and the organic isolate prevented the increase in malondialdehyde levels caused by dithranol treatment. The cytokine gene expressions elevated by dithranol exposure were reduced by the treatment. (4) Conclusions: Szigetvár medicinal water and organic substances alone may have a protective effect on patients’ healthy skin surfaces against dithranol damage. We also demonstrated that the organic compounds are also responsible for the protective effect. Full article

RNA-sequencing enables the comprehensive detection of gene expression levels at specific time points and facilitates the identification of stress-related genes through co-expression network analysis. Understanding the molecular mechanisms and identifying key genes associated with salt tolerance is crucial for developing rice varieties that can thrive in saline environments, particularly in regions affected by soil salinization. In this study, we conducted an RNA-sequencing-based time-course transcriptome analysis of ‘Jao Khao’, a salt-tolerant Thai rice variety, grown under normal or saline (160 mM NaCl) soil conditions. Leaf samples were collected at 0, 3, 6, 12, 24, and 48 h. In total, 36 RNA libraries were sequenced. ‘Jao Khao’ was found to be highly salt-tolerant, as indicated by the non-significant differences in relative water content, cell membrane stability, leaf greenness, and chlorophyll fluorescence over a 9-day period under saline conditions. Plant growth was slightly retarded during days 3–6 but recovered by day 9. Based on time-series transcriptome data, we conducted differential gene expression and weighted gene co-expression network analyses. Through centrality change from normal to salinity network, 111 key hub genes were identified among 1,950 highly variable genes. Enriched genes were involved in ATP-driven transport, light reactions and response to light, ATP synthesis and carbon fixation, disease resistance and proteinase inhibitor activity. These genes were upregulated early during salt stress and RT-qPCR showed that ‘Jao Khao’ exhibited an early upregulation trend of two important genes in energy metabolism: RuBisCo (LOC_Os10g21268) and ATP synthase (LOC_Os10g21264). Our findings highlight the importance of managing energy requirements in the initial phase of the plant salt-stress response. Therefore, manipulation of the energy metabolism should be the focus in plant resistance breeding and the genes identified in this work can serve as potentially effective candidates. Full article

Reaumuria genus (Tamaricaceae) is widely distributed across the desert and semi-desert regions of Northern China, playing a crucial role in the restoration and protection of desert ecosystems. Previous studies mainly focused on the physiological responses to environmental stresses; however, due to the limited availability of genomic information, the underlying mechanism of morphological and ecological differences among the Reaumuria species remains poorly understood. In this study, we presented the first catalog of expressed transcripts for R. kaschgarica, a sympatric species of xerophyte R. soongorica. We further performed the pair-wise transcriptome comparison to determine the conserved and divergent genes among R. soongorica, R. kaschgarica, and the relict recretohalophyte R. trigyna. Annotation of the 600 relatively conserved genes revealed that some common genetic modules are employed by the Reaumuria species to confront with salt and drought stresses in arid environment. Among the 250 genes showing strong signs of positive selection, eight pentatricopeptide repeat (PPR) superfamily protein genes were specifically identified, including seven PPR genes in the R. soongorica vs. R. trigyna comparison and one PPR gene in the R. kaschgarica vs. R. trigyna comparison, while the cyclin D3 gene was found in the R. soongorica vs. R. trigyna comparison. These findings suggest that genetic variations in PPR genes may affect the fertility system or compromise the extent of organelle RNA editing in R. trigyna. The present study provides valuable genomic information for R. kaschgarica and preliminarily reveals the conserved genetic bases for the abiotic stress adaptation and interspecific divergent selection in the Reaumuria species. The rapidly evolved PPR and cyclin D3 genes provide new insights on the endangerment of R. trigyna and the leaf length difference among the Reaumuria species. Full article

Soil salinity significantly impacts crop productivity. In response, plant growth-promoting rhizobacteria (PGPR) offer an innovative and eco-friendly solution to mitigate salinity stress. However, research on PGPR’s effects on crop physiology under varying salinity levels is still emerging. This study evaluates the impact of five bacterial strains, isolated from compost, on the growth of maize (Zea mays) and tomato (Solanum lycopersicum) plants under different levels of salt stress. This study involved treating maize and tomato seeds with five bacterial strains, and then planting them in a greenhouse under varying salt stress conditions (43 mM, 86 mM, 172 mM, 207 mM NaCl) using a Randomized Complete Block Design. Results showed that bacterial inoculation improved plant growth under saline conditions. S2015-1, S2026-2, and S2027-2 (Bacillus cereus, Acinetobacter calcoaceticus, Bacillus subtilis) were particularly effective in promoting plant growth under salt stress, especially at ionic concentrations of 43 mM and 86 mM, leading to a substantial increase in fresh and dry weight, with strain S2015-1 boosting chlorophyll by 29% at 86 mM in both crops. These results highlight the potential of PGPR to enhance crop resilience and productivity under salinity stress, promoting climate-smart agricultural practices. Full article

The Actin Depolymerizing Factor (ADF) protein, highly conserved among eukaryotes, is essential for plant growth, development, and stress responses. Cotton, a vital economic crop with applications spanning oilseed, textiles, and military sectors, has seen a limited exploration of its ADF gene family. This research has identified 118 unique ADF sequences across four principal cotton species: Gossypium hirsutum L., Gossypium barbadense Linn, Gossypium raimondii, and Asiatic cotton. The study found that the structural domains and physicochemical properties of these proteins are largely uniform across species. The ADF genes were classified into four subfamilies with a notable expansion in groups III and IV due to tandem and chromosomal duplication events. A thorough analysis revealed a high degree of conservation in gene structure, including exon counts and the lengths of introns and exons, with the majority of genes containing three exons, aligning with the characteristics of the ADF family. RNA-seq analysis uncovered a spectrum of responses by GhADFs to various abiotic stresses with GhADF19 showing the most significant reaction. Virus-induced gene silencing (VIGS) experiments were conducted to assess the role of GhADF19 in plant growth under abiotic stress. The results demonstrated that plants with silenced GhADF19 exhibited significantly slower growth rates and lower dry weights when subjected to cold, salt, and drought stress compared to the control group. This marked reduction in growth and dry weight under stress conditions highlights the potential importance of GhADF19 in stress tolerance mechanisms. Full article

Soil salinization is a widespread hindrance that endangers agricultural production and ecological security. High salt concentrations in saline soils are primarily caused by osmotic stress, ionic toxicity and oxidative stress, which have a negative impact on plant growth and development. In order to withstand salt stress, plants have developed a series of complicated physiological and molecular mechanisms, encompassing adaptive changes in the structure and function of various plant organs, as well as the intricate signal transduction networks enabling plants to survive in high-salinity environments. This review summarizes the recent advances in salt perception under different tissues, physiological responses and signaling regulations of plant tolerance to salt stress. We also examine the current knowledge of strategies for breeding salt-tolerant plants, including the applications of omics technologies and transgenic approaches, aiming to provide the basis for the cultivation of salt-tolerant crops through molecular breeding. Finally, future research on the application of wild germplasm resources and muti-omics technologies to discover new tolerant genes as well as investigation of crosstalk among plant hormone signaling pathways to uncover plant salt tolerance mechanisms are also discussed in this review. Full article