Search Results (3,814)

This study focuses on investigating sugar recovery from spoiled date fruits (SDF) for sustainable ethanol production using newly isolated yeasts. Upon their isolation from different food products, yeast strains were identified through PCR amplification of the D1/D2 region and subsequent comparison with the GenBank database, confirming isolates KKU30, KKU32, and KKU33 as Saccharomyces cerevisiae; KKU21 as Zygosaccharomyces rouxii; and KKU35m as Meyerozyma guilliermondii. Optimization of sugar extraction from SDF pulp employed response surface methodology (RSM), varying solid loading (20–40%), temperature (20–40 °C), and extraction time (10–30 min). Linear models for sugar concentration (R1) and extraction efficiency (R2) showed relatively high R² values, indicating a good model fit. Statistical analysis revealed significant effects of temperature and extraction time on extraction efficiency. The results of batch ethanol production from SDF extracts using mono-cultures indicated varying consumption rates of sugars, biomass production, and ethanol yields among strains. Notably, S. cerevisiae strains exhibited rapid sugar consumption and high ethanol productivity, outperforming Z. rouxii and M. guilliermondii, and they were selected for scaling up the process at fed-batch mode in a co-culture. Co-cultivation resulted in complete sugar consumption and higher ethanol yields compared to mono-cultures, whereas the ethanol titer reached 46.8 ± 0.2 g/L. Full article

One of the goals of modern orcharding is to produce a high volume of fruits with uniform size, organoleptic parameters, and health characteristics. The aim of this work was to study various shoot types and their prevailing positions along the cane and to identify how shoot type can influence the quality of fruit from the Actinidia tree. The experiment was conducted over a two-year period in a commercial orchard of Actinidia chinensis, cv. Gold 3. The shoots along the cane were classified as follows: spur shoots (SPs), terminated shoots (TEs), non-terminated shoots (NTs), and cut non-terminated shoots (CNTs). The data were statistically processed using ANOVA and Principal Component Analysis (ACP). Four different categories of fruit were obtained from the four shoot types, and their various attributes were compared. The prevailing category (comprising 55% of the studied fruits) was TEs, which are characterised by a higher soluble solid content, sweetness, and excellent health characteristics, as well as the reduced hardness of their pulp, which would support the hypothesis that harvesting could be brought forward. The second most common category (comprising 19% of total fruit) with the lowest soluble solid content, but a high antioxidant capacity, was that which was detached from the CNTs, while 13% of the fruit was produced from NTs, which had the lowest health value but good sweetness perception. Finally, the category with the lowest fruit percentage over the total fruit harvested (10%) was SPs, which are characterised by their smaller size. It has yet to be determined what the performance of each category will be post-harvest; whether it is possible to assign the quality categories while harvesting the fruit or to differentiate the harvest time accordingly remains subject to debate. Full article

This paper presents the findings of a study of the pelleting process of pine sawdust with the addition of waste in the form of potato pulp (as a natural binder), in the context of producing fuel pellets. The process of pelleting was carried out for sawdust and for a mixture of sawdust and potato pulp (10, 15, 20, and 25%). The highest moisture content was obtained in the case of pellets produced from a mixture of straw with a 25% potato pulp content, i.e., 26.54% (with a potato pulp moisture content of 85.08%). Increasing the potato pulp content in a mixture with sawdust from 10 to 25% reduced the power demand of the pelletizer by approx. 20% (from 7.35 to 5.92 kW). The obtained density values for pellets made from a mixture of sawdust and potato pulp (over 1000 kg∙m⁻³) with a potato pulp content of 10% make it possible to conclude that the obtained pellets meet the requirements of the ISO 17225-2:2021-11 standard. Increasing the potato pulp content from 0 to 25% caused a slight decrease in the heat of combustion, i.e., from 20.45 to 20.32 MJ∙kg⁻¹, as well as in the calorific value, from 19.02 to 18.83 MJ∙kg⁻¹ (both for dry sawdust matter and the mixture). The results of the laboratory tests were used to verify the densification process of mixtures of sawdust and potato pulp under industrial conditions at the PANBAH plant, using pelleting mixtures with a 5%, 10%, and 25% content of potato pulp. Industrial research also confirmed that the use of the addition of potato pulp in a mixture with sawdust significantly reduces the power demand of the pelletizer, and it also increases the kinetic strength of the obtained pellets. Full article

The tamarillo, or Solanum betaceum, recognized for its comprehensive nutritional profile, has long been valued for its diverse ethnobotanical uses. This study delves into the potential therapeutic applications of S. betaceum by analyzing its polyphenolic content (TPC), total flavonoid content (TFC), anti-skin aging activities against key enzymes like elastase, tyrosinase, and hyaluronidase, and its cytotoxic effects on oral carcinoma cells. Extracts from the seeds, pulp, and peel of red and yellow fruits were prepared using methanol, ethanol, and acetone. The highest TPC was found in the methanol extract from red fruit seeds (9.89 mg GAE/g), and the highest TFC was found in the methanol extract of yellow fruit peel (3.02 mg QUE/g). Some of these extracts significantly inhibited skin aging-associated enzymes with the red fruit seed extract (100 μg/mL) showing up to 50.4% inhibition of tyrosinase. Additionally, the red fruit seed extract obtained using methanol demonstrated potential anticancer effects against Ca9-22 oral carcinoma cells by inhibiting cell survival, migration, and proliferation as well as inducing apoptosis. These results underscore the potential of S. betaceum fruit extracts, especially from red fruit seeds, as promising agents for anti-skin aging and anticancer applications, meriting further exploration for therapeutic uses. Full article

Starch-based pH-sensing films with bacterial nanocellulose (BNC) and red cabbage anthocyanins (RCA) as active components were investigated in this research. Their structural, physical, surface and colorimetric properties were analyzed, mainly as a function of BNC concentration. The aim of the research was to relate the changes in the intermolecular interactions between the components of the films (starch, anthocyanins and BNC) to the physical, surface and colorimetric properties that are important for the primary intended application of the produced films as pH indicators in smart packaging. The results showed that maize starch (MS) was more suitable as a matrix for the stabilization of anthocyanins compared to potato starch (PS). PS-based films showed a lower value of water contact angle than MS-based films, indicating stronger hydrophilicity. The swelling behavior results indicate that the concentrations of BNC in MS-based films (cca 10%) and the concentration of about 50% BNC in PS-based films are required if satisfactory properties of the indicator in terms of stability in a wet environment are to be achieved. The surface free energy results of PS-based films with BNC were between 62 and 68 mJ/m² and with BNC and RCA between 64 and 68 mJ/m²; for MS-based films, the value was about 65 mJ/m² for all samples with BNC and about 68 mJ/m² for all samples with BNC and RCA. The visual color changes after immersion in different buffer solutions (pH 2.0–10.5) showed a gradual transition from red/pink to purple, blue and green for the observed samples. Films immersed in different buffers showed lower values of 2 to 10 lightness points (CIE L*) for PS-based films and 10 to 30 lightness points for MS-based films after the addition of BNC. The results of this research can make an important contribution to defining the influence of intermolecular interactions and structural changes on the physical, surface and colorimetric properties of bio-based pH indicators used in smart packaging applications. Full article

This study investigated how Rhus toxicodendron (RT) (6C, 30C, and 200C) can boost the immune system of BALB/c mice that were given cyclophosphamide (CPM), which is an anticancer drug that weakens the immune system. RT, known for its historical use in traditional homeopathic remedies, has demonstrated immunomodulatory and anti-inflammatory effects in various experimental models. To test the immune-boosting effects of RT, CPM (80 mg/kg) was given intraperitoneally to mice on days 4, 8, and 12 of the study but not to the normal control group. CPM-induced immunosuppression led to significant decreases in red blood cell (RBC), white blood cell (WBC), and hemoglobin (Hb) levels, and reduced spleen and thymus indices. Phagocytic activity, cytokine concentrations, and spleen architecture were also adversely affected. RT treatment, particularly at 200C, significantly ameliorated these effects, improving RBC, WBC, and Hb levels. Furthermore, RT partially prevented CPM-induced atrophy of immune organs. Treatment positively influenced cytokine production at both the protein and mRNA levels, restoring immune balance. Histopathological results confirmed that RT stimulated the immune system. The cells were more stable, and the white pulp in the spleen was arranged in a regular pattern. These findings suggest that RT may serve as an adjunctive immunostimulant therapy for conditions characterized by immunosuppression. However, further investigations in other immunocompromised states must validate these results before considering human clinical trials. Full article

(1) Background: The aim of this study was to compare the cytotoxicity of selected resin-modified materials used in direct contact with the dental pulp (TheraCal LC, TheraCal PT, and ApaCal ART) with calcium silicate cement (Biodentine). (2) Methods: The mouse fibroblast Balb/3T3 cell line and the extracts of tested materials in four concentrations were used for the testing. An MTT assay was performed in three independent experiments with six replicates for each concentration of tested material. The cell viability (%) and cytotoxicity were expressed (cytotoxic effect is considered in cases where the cell viability is lower than 70%). The mean of the cell viability and the standard deviation were expressed for each material at all concentrations. ANOVA and Dunnet’s post hoc tests were used for the statistical analysis. All of these tests were performed at the 0.05 significance level. (3) Results: At all concentrations, the cell viability was statistically significantly lower (p ≤ 0.002) for all tested materials compared to Biodentine. ApaCal ART showed a high level of cytotoxicity at all concentrations (cell viability lower than 47.71%, p < 0.0001). The same result was found for TheraCal LC at concentrations of 100%, 50% and 25% and TheraCal PT at concentrations of 100% and 50%. TheraCal LC at a 10% concentration (cell viability 68.18%) and TheraCal PT at a 25% concentration (cell viability 60.63%) indicated potential cytotoxicity. TheraCal PT at a 10% concentration was not found to be cytotoxic (cell viability 79.18%, p = 0.095). (4) Conclusion: The resin-modified calcium silicate and calcium phosphate materials showed higher cytotoxic potential, so they should be used with caution when in direct contact with the dental pulp. Full article

Mycoprotein is an alternative protein produced through fungal fermentation. However, it typically relies on refined glucose syrup derived from starch, which can be costly and unsustainable. This study investigates the potential of soybean processing by-products (okara and soy whey) as alternative substrates for producing mycoprotein using Aspergillus oryzae. A. oryzae was cultured for 7 days at 30 °C in diluted okara (1:50) and soy whey (1:1) with or without agitation (100 rpm). Soy whey produced higher biomass yields (369.2–408.8 mg dry biomass/g dry substrate), but had a lower biomass concentration (0.783–0.867 g dry weight/L). Conversely, okara produced a higher biomass concentration (2.02 g dry weight/L) with a yield of 114.7 mg dry biomass/g dry substrate. However, biomass formation in okara was only observed in static conditions, as agitation caused biomass to entangle with soy pulp, hampering its production. Additionally, okara tended to release protein into the media, while soy whey accumulated protein within the biomass, reaching up to 53% w/w protein content. The results of this study provide a promising approach to addressing both soybean processing waste reduction and food security concerns. Full article

Instant Cascara (IC) is a sustainable beverage made from dried coffee cherry pulp, a by-product of coffee processing. It is rich in nutrients and bioactive compounds and has a high concentration of antioxidants. This study explored the impact of regular IC consumption on colonic motor function and innervation. Over a period of 4 weeks, male and female healthy rats were given drinking water containing 10 mg/mL of IC. Thereafter, colon samples were obtained to evaluate the longitudinal (LM) and circular (CM) smooth muscle contractile response to acetylcholine (ACh) and electrical field stimulation (EFS) in an organ bath, before and after atropine administration (10⁻⁶ M). Histological and immunohistochemical analyses assessed colon damage, muscle thickness, and immunoreactivity to substance P (SP) and neuronal nitric oxide synthase (nNOS). ACh and EFS induced similar responses across groups, but the CM response to EFS was greater in females compared with males, despite their lower body weight. Atropine completely blocked the response to ACh but only partially antagonized the neural response to EFS, particularly that of CM in females treated with IC, which had a greater liquid intake than those exposed to water. However, in the myenteric ganglia, no statistically significant differences were observed in SP or nNOS. Our results suggest that regular IC exposure may enhance specific neural pathway functions, particularly in females, possibly due to their increased IC consumption. Full article

Sand mining tailing (ST) is a byproduct of the sand extraction of submerged pits, a process which is carried out to obtain aggregates for civil construction. This tailing consists of fine particles from the pulp washing process, usually disposed of in decantation ponds. The present study proposes ST as a supplementary cementitious material (SCM) for Portland cement concrete, thereby reintegrating this tailing into the production chain. In this sense, ST was characterized, and concretes containing 2% to 14% of cement replacement (%vol) by ST were produced and evaluated. STs showed natural fineness, particles with angular morphology, a significant amount of kaolinite, and 36% amorphous content. ST concretes exhibited a compressive strength of up to 57.9 MPa at 28 days under 7.0% of cement replacement, 38.8% higher than the reference. Consequently, only 5.6 kg/m³ of Portland cement was required to attain 1.0 MPa, representing a 33.6% reduction compared to the reference. The ultrasonic pulse velocities measured in ST concrete with 2.0%, 4.0%, 7.0%, and 14.0% cement replacement were 3.0%, 6.1%, 9.3%, and 6.6% higher than the reference, respectively. These results indicate enhanced mechanical properties, improved matrix uniformity, and superior environmental performance across all SCM levels compared to the reference, with optimal efficiency observed at 7.0% ST content. Full article

The objective of this study was to analyze the chemical profile of extracts from different fruit parts of the perennial arborescent cactus Opuntia leucotricha by applying ultra-high-performance liquid chromatography–high-resolution mass spectrometry (UHPLC-HRMS) and to evaluate their biological properties (antioxidant and enzyme inhibitory capacities). Extracts were obtained from the fruit pulp (without or with seeds), seeds, and peel by ultrasound-assisted extraction (UAE) using the conventional solvent methanol 50% and two Natural Deep Eutectic Solvents (NADESs) (glycerol:urea, 1:1 and citric acid:sorbitol, 1:2). A total of 33 compounds were identified, including phenolics, fatty acids, and others. Phenolic acids were the most abundant class of phenolics identified in all fruit parts, with the highest concentration observed in the methanol extracts from peel (593.02 µg/g_DW), followed by seed-containing pulp (69.03 µg/g_DW), pulp (57.83 µg/g_DW), and seeds (39.97 µg/g_DW). The second most effective extractant was NADES 1 (glycerol:urea, 1:1), which was also successful in extracting compounds with antioxidant capacity. Overall, the extracts demonstrated considerable enzyme inhibitory activities, with the greatest effects observed against α-amylase and α-glucosidase. The results indicate that O. leucotricha fruits could be a promising source of bioactive compounds, and NADES a viable alternative to organic solvents for their industrial exploitation. Full article

This study evaluates the effects of substituting cassava pulp with broken rice and cassava chips in the total mixed ration silage diets of beef cattle on feed composition, ensiling quality, digestibility, and energy utilization. Fifteen Holstein Thai native crossbred (89% Bos taurus × 11% Bos indicus) steers in the fattening phase, with an average age of 2.5 ± 0.1 years and an initial body weight of 603.7 ± 14.3 kg, were used in the energy balance trial. Using a randomized complete block design with five replications, the steers received one of three treatments. The three dietary treatments included substituting cassava pulp with cassava chips and broken rice on a dry matter basis with ratios of 50:0:0, 30:20:0, or 10:20:20. The results show that broken rice is a superior nutrient source and provides greater energy balance (p < 0.01). Despite the cost implications, substituting cassava pulp and chips positively impacts the ensilage pH and reduces the acetic acid concentration (p < 0.01). There was an increase in the lactic acid bacteria count (p < 0.05) and a reduction in the rumen ammonia, propionate, and butyrate concentrations (p < 0.05) without adverse effects (p > 0.05) on digestibility, blood metabolites, or enteric methane emissions. These findings suggest that broken rice is a promising alternative grain-rich ruminant feed. Future research should explore on-farm long-term feeding and economic evaluations to provide a more comprehensive understanding of the practical implications. Full article

The research results and evaluation of the applicability of the original composition of hybrid paper based on silver nanowires (AgNWs), cellulose pulp (CP), and carbon nanotubes (CNTs) are presented and discussed. The material tested was used to manufacture sensors for mechanical deformation resulting from external influences or related to human activity interactions. The sensors were fabricated using an AgNWs + CP suspension and additives by the vacuum filtration method. The substrate obtained was machined and then laminated with a layer of poly(dimethylsiloxane) (PDMS). The recorded responses to selected types of imposed mechanical interactions in the form of changes in the relative resistance of the sensor throughout the tests showed a close cause-and-effect relationship. The response of the tested systems when applying an alternating magnetic field was also observed. The results indicate that the proposed solutions can find application in the monitoring of mechanical interactions resulting from the dynamic behavior of physical objects, as well as derived from selected human vital functions. Full article

This study aims to develop purple-coloured polymeric coatings using natural anthocyanin and desoxyanthocianidins (3-DXA) colourants for application to chocolate almonds. The objective is to achieve a stable and uniform colour formulation throughout processing and storage, enhancing the appearance and durability of the almonds to appeal to health-conscious consumers and align with market demands. Plant materials like sweet potato pulp, sweet potato peel, radish peel, black carrot, and sorghum were employed to obtain the desired purple hue. Anthocyanidins and 3-DXA were extracted from the matrices using solvent extraction and ultrasound-assisted methods at different pH values. High-performance liquid chromatography with diode array detection (HPLC-DAD) and high-resolution tandem mass spectrometry (HRMS/MS) were used to identify the compounds in the extracts. The highest antioxidant capacities, as measured by the DPPH^• and FRAP methods, were observed in purple sweet potato and dye factory extracts, respectively; meanwhile, sorghum extract inhibited both α-amylase and α-glucosidase, indicating its potential for managing postprandial hyperglycemia and type 2 diabetes. The degradation kinetics of coloured coatings in sugar syrup formulations with anthocyanins and 3-DXA revealed that locust bean gum offered the best colour stabilization for plant extracts, with sorghum extracts showing the highest and black carrot extracts the lowest colour variation when coated with Arabic gum. Sweet potato pulp extracts exhibited less colour variation in sugar pastes, both with and without blue spirulina dye, compared to factory dye, highlighting their potential as a more stable and suitable alternative for colouring purple almonds, particularly over a five-month storage period. This study supports sustainable practices in the confectionery industry while aligning with consumer preferences for healthier and environmentally friendly products. Full article

Periapical lesions are common pathologies affecting the alveolar bone, often initiated by intraradicular lesions resulting from microbial exposure to dental pulp. These microorganisms trigger inflammatory and immune responses. When endodontic treatment fails to eliminate the infection, periapical lesions persist, leading to bone loss. The RANK/RANKL/OPG pathway plays a crucial role in both the formation and the destruction of the bone. In this study, the objective was to inhibit the RANK/RANKL pathway in vitro within exposed Thp-1 macrophages to endodontic microorganisms, specifically Enterococcus faecalis, which was isolated from root canals of 20 patients with endodontic secondary/persistent infection, symptomatic and asymptomatic, and utilizing an α-IRAK-4 inhibitor, we introduced endodontic microorganisms and/or lipoteichoic acid from Streptococcus spp. to cellular cultures in a culture plate, containing thp-1 cells and/or PBMC from patients with apical periodontitis. Subsequently, we assessed the percentages of RANK+, RANKL+, and OPG+ cells through flow cytometry and measured the levels of several inflammatory cytokines (IL-1β, TNF-α, IL-6, IL-8, IL-10, and IL-12p70) in the cellular culture supernatant through a CBA kit and performed analysis by flow cytometry. A significant difference was observed in the percentages of RANK+RANKL+, OPG+ RANKL+ cells in thp-1 cells and PBMCs from patients with apical periodontitis. The findings revealed significant differences in the percentages of the evaluated cells, highlighting the novel role of the IRAK-4 inhibitor in addressing this oral pathology, apical periodontitis, where bone destruction is observed. Full article