Search Results (31)

Threonine phosphorylation promotes inflammatory functions of STAT1 while restricting its interferon (IFN) signaling in innate immune responses. However, it remains unclear whether the restriction of STAT1-mediated IFN signaling conferred by threonine phosphorylation is a ubiquitous mechanism or one that is context-dependent. To address this, we utilized pristane-induced lupus, a prototype IFN-driven systemic autoimmune disease model characterized by the production of high-titer autoantibodies against nucleic acid-associated antigens. Through genetic and biochemical assays, we demonstrate that Thr748 phosphorylation is dispensable for STAT1 functionality in pristane-induced lupus. Genetically engineered mice expressing the phospho-deficient threonine 748-to-alanine (T748A) mutant STAT1 exhibited similar survival rates, high titers of anti-dsDNA IgG, and nephritis compared to their wild-type littermates. In sharp contrast, STAT1 deficiency protected mice against pristane-induced lupus, as evidenced by increased survival, low titers of anti-dsDNA IgG, and less severe nephritis in the STAT1 knockout mice compared to their T748A littermates. Our study suggests a phosphorylation-dependent modularity that governs the spectrum of STAT1 functionality in inflammatory contexts: IFN phospho-tyrosine-dependent and inflammatory phospho-threonine-dependent, with Thr748 phosphorylation driving selective inflammatory activities, particularly those not driven by the canonical JAK pathway. From a broader perspective, our findings provide deeper insights into how distinct phosphorylation events shape the combinatorial logic of signaling cassettes, thereby regulating context-dependent responses. Full article

It is not entirely clear how the interaction between joint inflammation and the central nervous system (CNS) response in rheumatoid arthritis (RA) works, and what pathophysiology underlies the sex differences in coexisting neuropsychiatric comorbidities. It is known that estrogen hormones reduce inflammation in RA and that this occurs mainly via the stimulation of G protein-coupled receptor-30 (GPR30), also known as G protein-coupled estrogen receptor (GPER) 1. However, changes in GPR30 expression and sex differences induced by local and systemic inflammation in RA are not yet known. Our aim was to reveal sex differences in the expression and association of joint GPR30 with local and systemic inflammation, clinical course and furthermore with hippocampal GPR30 expression during pristane-induced arthritis (PIA) in Dark Agouti (DA) rats, an animal model of RA. Furthermore, we demonstrated sex-specific differences in the association between joint and systemic inflammation and hippocampal microglia during PIA. Our results suggest sex-specific differences not only in the clinical course and serum levels of pro-inflammatory cytokines but also in the expression of GPR30. Female rats show greater synovial inflammation and greater damage to the articular cartilage compared to males during PIA attack. Male rats express higher levels of synovial and cartilaginous GPR30 than females during PIA, which correlates with a less severe clinical course. The correlation between synovial and cartilaginous GPR30 and joint inflammation scores (Krenn and Mankin) in male rats suggests that the more severe the joint inflammation, the higher the GPR30 expression. At the same time, there is no particular upregulation of hippocampal GPR30 in males. On the other hand, female rats express higher levels of neuroprotective GPR30 in the hippocampus than male rats at the basic level and during PIA attack. In addition, females have a higher number of Iba-1+ cells in the hippocampus during PIA attack that strongly correlates with the clinical score, serum levels of IL-17A, and Krenn and Mankin scores. These results suggest that male rats are better protected from inflammation in the joints and female rats are better protected from the inflammation in the hippocampus during a PIA attack, independently of microglia proliferation. However, in the remission phase, synovial GPR30 expression suddenly increases in female rats, as does hippocampal GPR30 expression in males. Further experiments with a longer remission period are needed to investigate the molecular background of these sex differences, as well as microglia phenotype profiling. Full article

The Ecuadorian Amazon rainforest stands out as one of the world’s most biodiverse regions, yet faces significant threats due to oil extraction activities dating back to the 1970s in the northeastern provinces. This research investigates the environmental and societal consequences of prolonged petroleum exploitation and oil spills in Ecuador’s Amazon. Conducted in June 2015, the study involved a comprehensive analysis of freshwater sediment samples from 24 locations in the Rio Aguarico and Napo basins. Parameters such as water and air temperature, conductivity, soil pH, and hydrocarbon concentrations were examined. Total petroleum hydrocarbon (TPH) concentrations ranged from 9.4 to 847.4 mg kg⁻¹, with polycyclic aromatic hydrocarbon (PAH) levels varying from 10.15 to 711.1 mg kg⁻¹. The pristane/phytane ratio indicated historic hydrocarbon pollution in 8 of the 15 chemically analyzed sediments. Using non-culturable techniques (Illumina), bacterial analyses identified over 350 ASV, with prominent families including Comamonadaceae, Chitinophagaceae, Anaeromyxobacteraceae, Sphingomonadaceae, and Xanthobacteraceae. Bacterial diversity, assessed in eight samples, exhibited a positive correlation with PAH concentrations. The study provides insights into how microbial communities respond to varying levels of hydrocarbon pollution, shedding light on the enduring impact of oil exploitation in the Amazonian region. Its objective is to deepen our understanding of the environmental and human well-being in the affected area, underscoring the pressing need for remedial actions in the face of ongoing ecological challenges. Full article

Kazakhstan ranks as the 12th largest oil producer globally and boasts a diverse range of crude oils. This research introduces a method for distinguishing between the different types of crude oils based on biomarker analysis of 28 crude oils from Western and Southern Kazakhstan using gas chromatography-mass spectrometry. Biomarkers serve as valuable tools, especially in forensic investigations of oil spills. These biomarkers effectively retain a significant portion of the original natural product’s carbon structure, providing crucial evidence regarding the origin and identity of the oils under examination. This study identifies a set of biomarkers, including pristine, phytane, n-C₁₇ and n-C₁₈ alkanes, hopanes, bisnorhopanes, iso-copalanes, pregnane, androstane, allopregnane, homopregnane, cholestane, and stigmastane. By examining ratios such as pristane/phytane, pristane/n-C₁₇ alkane, tricyclic/pentacyclic terpanes, and hopane, as well as the distribution of steranes, it was deduced that crude oils from West Kazakhstan exhibited resilience to biodegradation. These findings showed that gas chromatography-mass spectrometry is an effective method for oil biomarkers determination, especially because it provides efficient separation and identification. Additionally, this study delved into the origin conditions and maturity of these oils, contributing to a deeper understanding of their characteristics and analysis that is simple to use and available worldwide. Full article

For decades, researchers have focused on containing terrestrial oil pollution. The heterogeneity of soils, with immense microbial diversity, inspires them to transform pollutants and find cost-effective bioremediation methods. In this study, the mycoremediation potentials of five filamentous fungi isolated from polluted soils in Kazakhstan were investigated for their degradability of n-alkanes and branched-chain alkanes as sole carbon and energy sources. Dry weight estimation and gas chromatography–mass spectrometry (GC-MS) monitored the growth and the changes in the metabolic profile during degradation, respectively. Penicillium javanicum SBUG-M1741 and SBUG-M1742 oxidized medium-chain alkanes almost completely through mono- and di-terminal degradation. Pristane degradation by P. javanicum SBUG-M1741 was >95%, while its degradation with Purpureocillium lilacinum SBUG-M1751 was >90%. P. lilacinum SBUG-M1751 also exhibited the visible degradation potential of tetradecane and phytane, whereby in the transformation of phytane, both the mono- and di-terminal degradation pathways as well as α- and ß-oxidation steps could be described. Scedosporium boydii SBUG-M1749 used both mono- and di-terminal degradation pathways for n-alkanes, but with poor growth. Degradation of pristane by Fusarium oxysporum SBUG-M1747 followed the di-terminal oxidation mechanism, resulting in one dicarboxylic acid. These findings highlight the role of filamentous fungi in containing oil pollution and suggest possible degradation pathways. Full article

The crude oils from the reservoirs of Mela-01 and Mela-04 wells located in the Kohat Basin, Pakistan, were geochemically analyzed to evaluate the origin, depositional conditions, and thermal maturity of the rock units and possible facies from which these oils were sourced. Gas chromatography-mass spectrometry (GC-MS) was performed on the samples to obtain biomarker and non-biomarker parameters. Analyzed non-biomarker parameters, including carbon preference index (CPI), terrigenous to aquatic ratio (TAR), isoprenoids pristane to phytane (Pr/Ph), and biomarker parameters, including steranes and dibenzothiophene/phenanthrene (DBT/P) of aromatic compounds, were utilized in the present study to achieve the objectives. Most of these parameters suggest a mixed source of organic matter (marine/terrestrial) with sub-oxic conditions in the source rocks for the analyzed oil samples in the studied wells from Mela oilfield, Kohat Basin. Furthermore, the CPI and different biomarker parameters such as steranes C₂₉ S/S + R, ββ/αα + ββ), moretane to hopane (M₂₉/C₃₀H), pentacyclic terpanes C₂₇ (Ts/Ts + Tm), H₃₂ (S/S + R) hopanes, and aromatic methylphenanthrene index (MPI) indicate that the analyzed oils have originated from thermally mature rocks falling in the oil window. As the studied Kohat Basin has multiple source rocks and contributes to the major petroleum production of the country, the present investigations reveal that its okthe Mela oils were generated by the strata of mixed organic matter that were deposited in marine sub-oxic conditions. Furthermore, this study suggests that this stratum would also have been produced in unexplored surrounding areas such as Tirah, Orakzai, and the Bannu Depression. Full article

Systemic lupus erythematosus (SLE) is a chronic immune-inflammatory disease characterized by multiorgan affectation and lowered self-tolerance. Additionally, epigenetic changes have been described as playing a pivotal role in SLE. This work aims to assess the effects of oleacein (OLA), one of the main extra virgin olive oil secoiridoids, when used to supplement the diet of a murine pristane-induced SLE model. In the study, 12-week-old female BALB/c mice were injected with pristane and fed with an OLA-enriched diet (0.01 % (w/w)) for 24 weeks. The presence of immune complexes was evaluated by immunohistochemistry and immunofluorescence. Endothelial dysfunction was studied in thoracic aortas. Signaling pathways and oxidative-inflammatory-related mediators were evaluated by Western blotting. Moreover, we studied epigenetic changes such as DNA methyltransferase (DNMT-1) and micro(mi)RNAs expression in renal tissue. Nutritional treatment with OLA reduced the deposition of immune complexes, ameliorating kidney damage. These protective effects could be related to the modulation of mitogen-activated protein kinases, the Janus kinase/signal transducer and transcription activator of transcription, nuclear factor kappa, nuclear-factor-erythroid-2-related factor 2, inflammasome signaling pathways, and the regulation of miRNAs (miRNA-126, miRNA-146a, miRNA-24-3p, and miRNA-123) and DNMT-1 expression. Moreover, the OLA-enriched diet normalized endothelial nitric oxide synthase and nicotinamide adenine dinucleotide phosphate (NADPH) oxidase-1 overexpression. These preliminary results suggest that an OLA-supplemented diet could constitute a new alternative nutraceutical therapy in the management of SLE, supporting this compound as a novel epigenetic modulator of the immunoinflammatory response. Full article

Systemic lupus erythematosus (SLE) is a systemic autoimmune disease with enhanced NETosis and impaired degradation of neutrophil extracellular traps (NETs). Galectin-3 is a β-galactoside binding protein and is associated with neutrophil functions as well as involved in mediating autoimmune disorders. In this study, we plan to examine the associations of galectin-3 with the pathogenesis of SLE and NETosis. Galectin-3 expression levels were determined in peripheral blood mononuclear cells (PBMCs) of SLE patients for the association with lupus nephritis (LN) or correlation of SLE disease activity index 2000 (SLEDAI-2K). NETosis was observed in human normal and SLE and murine galectin-3 knockout (Gal-3 KO) neutrophils. Gal-3 KO and wild-type (WT) mice induced by pristane were used to evaluate disease signs, including diffuse alveolar haemorrhage (DAH), LN, proteinuria, anti-ribonucleoprotein (RNP) antibody, citrullinated histone 3 (CitH3) levels, and NETosis. Galectin-3 levels are higher in PBMCs of SLE patients compared with normal donors and positively correlated with LN or SLEDAI-2K. Gal-3 KO mice have higher percent survival and lower DAH, LN proteinuria, and anti-RNP antibody levels than WT mice induced by pristane. NETosis and citH3 levels are reduced in Gal-3 KO neutrophils. Furthermore, galectin-3 resides in NETs while human neutrophils undergo NETosis. Galectin-3-associated immune complex deposition can be observed in NETs from spontaneously NETotic cells of SLE patients. In this study, we provide clinical relevance of galectin-3 to the lupus phenotypes and the underlying mechanisms of galectin-3-mediated NETosis for developing novel therapeutic strategies targeting galectin-3 for SLE. Full article

Field observations, together with the results of gas chromatography–mass spectrometry (GC-MS) and stable carbon isotope analysis of bitumen, coupled with fluid inclusion microthermometry and stable isotope analyses of closely associated vug- and fracture-filling columnar calcite in the Upper Cretaceous Bekhme Formation, Kurdistan Region of Iraq, suggest that the degradation of crude oil was caused by the regional incursion of meteoric waters. This incursion, which is interpreted to have occurred during tectonic uplift during the Zagros Orogeny, is evidenced by: (i) the depletion of n-alkanes and acyclic isoprenoid alkanes (pristane and phytane) in the bitumen; (ii) low δ¹³C_VPDB values (−8.5‰ to −3.9‰) and δ¹⁸O_VPDB values (−22.9‰ to −15.0‰), with more radiogenic Sr isotopic ratios (0.70771–0.70772) compared to Cretaceous seawater; and (iii) low salinity and low temperatures (20 to 40 °C) in fluid inclusions of the columnar calcite. This study demonstrates that regional meteoric water incursion into sedimentary basins can be linked to crude oil degradation accompanied by calcite cementation events in carbonate reservoirs. Full article

Coix lachryma-jobi L. is an excellent plant resource that has a concomitant function for medicine, foodstuff and forage in China. At present, the commonly used cultivar for both medicine and foodstuff is Xiaobaike, and the cultivar for foraging is Daheishan. However, differences in the internal composition of plants lead to the expression of different phenotypic traits. In order to comprehensively elucidate the differences in nutrient composition changes in Coix seeds, a non-targeted metabolomics method based on ultra-performance liquid chromatography quadrupole time-of-flight mass spectrometry (LC-Q-TOF-MS) was used to analyze the metabolic changes in Coix seeds at different developmental stages. An edible Coix relative (Xiaobaike) and a feeding Coix relative (Daheishan) were selected as the research subjects. In the metabolome analysis of Coix seed, 314 metabolites were identified and detected, among which organic acids, carbohydrates, lipids, nucleotides and flavonoids were the main components. As an important standard for evaluating the quality of Coix seed, seven lipids were detected, among which fatty acids included not only even-chain fatty acids, but also odd-chain fatty acids, which was the first time detecting a variety of odd-chain fatty acids in Coix seed. The analysis of the compound contents in edible and feeding-type Coix lachryma-jobi L. and the lipid content at the mature stage showed that, among them, arachidic acid, behenic acid, heptadecanoic acid, heneicosanoic acid and pristanic acid may be the key compounds affecting the lipid content. In addition, in the whole process of semen coicis maturation, edible and feeding Coix show similar trends, and changes in the third period show clear compounds in the opposite situation, suggesting that edible and feeding Coix not only guarantee the relative stability of species but also provide raw materials for genetic breeding. This study provides valuable information on the formation of the edible and medicinal qualities of Coix. Full article

Yarrowia lipolytica is a unique, strictly aerobic yeast with the ability to degrade efficiently hydrophobic substrates. In the present work, we evaluated the degrading potential of Yarrowia lipolytica IMUFRJ 50682, isolated from tropical estuarine water in Rio de Janeiro (Brazil), and the possible biomolecules produced during this process. To investigate which crude oil compounds are degraded by Y. lipolytica IMUFRJ 50682, this microorganism was grown in a medium containing Marlim petroleum (19 °API, American Petroleum Institute gravity) at 28 °C and 160 rpm for 5 days. The residual petroleum was submitted to gas chromatograph-mass spectrometric analysis (GC-MS). The chromatographic fingerprints of the residual petroleum were compared with the abiotic control test incubated in the same conditions. Y. lipolytica assimilates high molecular weight hydrocarbons, such as n-alkanes (C11-C19), isoprenoids (pristane and phytane), aromatics with two or three aromatics rings (naphthalene, methylnaphthalenes, dimethylnaphthalenes, trimethylnaphthalenes, phenanthrene, methylphenanthrenes, dimethylphenanthrenes, anthracene). This strain was also capable of consuming more complex hydrocarbons, such as tricyclic terpanes. During this biodegradation, the emulsification index of the culture medium increased significantly, showing that biosurfactant molecules can be produced from this process. Therefore, Y. lipolytica IMUFRJ 50682 showed to be a potential crude oil degrading yeast, which can be used for bioremediation processes and simultaneously produce bioproducts of commercial interest. Full article

In the present study, a high-efficiency petroleum hydrocarbon-degrading bacterium MX1 was screened from petrochemical wastewater sludge, and MX1 was identified using morphological, physiological, and biochemical experiments and combined with 16S rDNA. Results showed that the the MX1 strain belongs to Enterobacter sp. The degradation conditions were an incubation time of 18 days, temperature of 30 °C, pH of 7, and salinity of 2% (w/v), and the degradation proportion was 37.41% for 7 days. The combination of microwave and ultraviolet mutagenesis yielded the strain MXM3U2. The mutant strain had a petroleum hydrocarbon breakdown efficiency of 56.74% after 7 days of culture, and this value was 51.66% higher than the original strain. The number of strains and the rate of degradation of n-alkanes (C16, C24, C32, and C40) decreased steadily with the increase in carbon chains in the degradation test. GC/MS (Gas chromatography mass spectrometry) results showed that in the process of degrading crude oil, the hydrocarbons with carbon number C < 24 were degraded first, followed by hydrocarbons with carbon number C > 24. The strains had a good degradation effect on pristane, naphthalene, and phenanthrene. In this study, a high-efficiency petroleum hydrocarbon-degrading bacterium was screened via microwave-ultraviolet composite mutagenesis technology. Full article

The presence of heavy metals in plants from oil sand deposits may reflect mineralization resulting from petroleum biodegradation. Petroleum composition and heavy metal analyses were performed using thermal desorption gas chromatography and atomic absorption spectrophotometry on oil sand and plant root samples from the same localities in the Dahomey Basin. The results from the oil sand showed mainly heavy-end hydrocarbon components, humps of unresolved complex mixtures (UCM), absences of C6-C12 hydrocarbon chains, pristane, and phytane, indicating severe biodegradation. In addition, they showed varying concentrations of vanadium (2.699–7.708 ppm), nickel (4.005–11.716 ppm), chromium (1.686–5.733 ppm), cobalt (0.953–3.223 ppm), lead (0.649–0.978 ppm), and cadmium (0.188–0.461 ppm). Furthermore, these heavy metals were present in Citrus, Theobroma Cacao, Elaeis guineensis, and Cola. The chromium, nickel, vanadium, lead, cobalt, and cadmium concentrations in the Citrus were 7.475, 4.981, 0.551, 0.001, 0.806, and 0.177 ppm, respectively. For the Theobroma Cacao, the concentrations of chromium, nickel, vanadium, lead, cobalt, and cadmium were 7.095, 16.697, 2.151, 0.023, 3.942, and 0.254 ppm. Elaeis guineensis also showed the presence of chromium (32.685 ppm), nickel (32.423 ppm), vanadium (11.983 ppm), lead (0.190 ppm), cobalt (4.425 ppm), and cadmium (0.262 ppm). The amounts of chromium, nickel, vanadium, lead, cobalt, and cadmium in the Cola were 9.687, 9.157, 0.779, 0.037, 0.695, and 0.023 ppm. The World Health Organization’s (WHO) safe and permissible limits for Cd (0.003 ppm), Cr (0.1 ppm), Ni (0.05 ppm), and Pb (0.1 ppm) in agricultural soils were all exceeded in the oil sand. The presence of these metals in the oil sands and their uptake by the plants could potentially be toxic, resulting in high mortality. The metal–metal correlation of the plant’s rootsto the oil sand indicates the nonanthropogenic origin of the heavy metals, which leads to the conclusion that their source is related to the hydrocarbon accumulation in the Afowo sand. Full article

Coal and organic-rich shale in the Yan’an Formation in the southwestern margin of the Ordos Basin are widely developed, which is an important fact for oil and gas exploration in China that has been widely explored for a long time. In this paper, detailed sequence division and sedimentary environment analyses of the Yan’an Formation in the Pengyang area on the southwestern margin of the Ordos Basin were conducted using field outcrops, drilling cores, logging, wavelet transform and organic geochemistry. The results showed that the succession consists of some units with distinctly different characteristics. Based on the petrographic assemblage and transform wavelet characteristics, the Yan’an Formation in this area can be divided into a long-term cycle, five medium-term cycles, and eleven short-term cycles, among which coal and carbonaceous shale were mainly developed in the short-term cycles I₂, III₁, III₂, V₁ and V₂. Coal and organic-rich mud shale have been developed in the Yan’an Formation and plant debris in mudstone and coal is common, indicating the development of swamps and shallow water-covered depressions in this area. The sandstones showed parallel bedding, cross-bedding and scours, thus indicating fluvial deposits. The saturated hydrocarbon gas chromatographic parameters of mud shale showed that the pristane/phytane (Pr/Ph) ratio is 2.24–6.22, the Ph/nC18 ratio is 0.15–0.93, and the Pr/nC17 ratio is 0.97–2.78, supporting the finding that the organic matter has mainly originated from terrestrial sources. Full article

For the identification of antigenic protein biomarkers for rheumatoid arthritis (RA), we conducted IgG profiling on high density protein microarrays. Plasma IgG of 96 human samples (healthy controls, osteoarthritis, seropositive and seronegative RA, n = 24 each) and time-series plasma of a pristane-induced arthritis (PIA) rat model (n = 24 total) were probed on AIT’s 16k protein microarray. To investigate the analogy of underlying disease pathways, differential reactivity analysis was conducted. A total of n = 602 differentially reactive antigens (DIRAGs) at a significance cutoff of p < 0.05 were identified between seropositive and seronegative RA for the human samples. Correlation with the clinical disease activity index revealed an inverse correlation of antibodies against self-proteins found in pathways relevant for antigen presentation and immune regulation. The PIA model showed n = 1291 significant DIRAGs within acute disease. Significant DIRAGs for (I) seropositive, (II) seronegative and (III) PIA were subjected to the Reactome pathway browser which also revealed pathways relevant for antigen presentation and immune regulation; of these, seven overlapping pathways had high significance. We therefore conclude that the PIA model reflects the biological similarities of the disease pathogenesis. Our data show that protein array analysis can elucidate biological differences and pathways relevant in disease as well be a useful additional layer of omics information. Full article