Search Results (169)

(1) Background: Since older adults are more susceptible to dehydration and there is a lack of information on older athletes, this study observed a group of 12 male padel players in this age group (70.42 ± 3.50 years) to characterize their hydration habits, physiological demands, and psychological responses before and during a 90 min padel training (PT). (2) Methods: After approval from the Ethics Committee (CE/FCDEF-UC/00022023) and the provision of signed informed consent, participants’ body mass, height, waist and hip circumferences, body mass index, waist-to-hip ratio, and waist-to-height ratio were measured. Habitual fluid intake was monitored by diary from the evening until before the PT; the subjects completed a Profile of Mood States questionnaire (POMS) and a satiety scale (SLIM). To assess hydration levels at different moments, we used a portable osmometer and an eight-point urine color chart and weighed the participants immediately before and after the PT. During the PT, heart rate (HR) and hydration were monitored. After the PT, subjects completed another POMS and SLIM. (3) Results: Subjects trained at 73.2 ± 12.3% of their maximum HR, with brief peaks at the anaerobic threshold or higher (130.00 ± 18.78 bpm). The mean urine osmolality indicated normal hydration or minimal dehydration. However, the urine color values indicated dehydration after the training. Subjects drank 438 mL of liquids at night, 333 mL before PT, and 900 mL during the PT, with a good repartition of the liquids. POMS and SLIM were not affected by the training. (4) Conclusions: Older male padel athletes achieved challenging yet safe training, staying within healthy intensity zones; their hydration patterns nearly met the recommendations for exercise and should be slightly increased. Full article

Obtaining cellulose from forest residues develops sustainable processes in the biotechnology industry, especially in producing biopolymers, which could replace or add petroleum-derived polymers. This research seeks to optimize the ideal conditions of the mixing process to maximize the efficiency in obtaining cellulose through a process consisting of two treatment media for pine sawdust, specifically evaluating the impact of three types of impellers (propeller, flat blades, and 45° inclined flat blades) at speeds of (150, 250 and 350 rpm). DIN 28131 was used for the design of stirred tanks. Simulations were carried out with a volume of 50 L. CFD and FSI simulations of the agitation behavior of forest biomass in a stirred tank reactor were performed. The ALE method was applied, and the models were solved using the LS-DYNA computer program. The results indicate that agitation with propellers and flat blades inclined at 150 and 250 rpm was the most efficient, minimizing cell damage and optimizing energy consumption. The impeller with flat blades inclined at 45° proved to be the best option for cellulose extraction. The novelty of this research is that not only the flow fields and the agitation behavior were found, but also the stresses in the impellers were found, and the force, moment, and power required by the motor in each simulation were revealed at a different speed. The power curves shown help to understand how energy consumption varies under different conditions. Full article

To meet growing energy demands, offshore wind turbines (OWTs) with higher energy outputs are being developed, presenting increased challenges for their foundation design. Over the past decade, extensive research on the design optimization of OWT support structures has significantly reduced the anticipated costs of offshore wind farm development. Various design methods have been developed and applied in practice, each with its own advantages and limitations. In this study, 3D finite element (FE) modeling, validated against the measured response of a large-scale test monopile, is used to investigate the lateral load response of monopiles with different geometries and slenderness ratios in smaall and large displacements. The results are compared to the standard p–y method, and specific behavioral and design aspects of large-diameter monopiles, such as the moment contribution ratio from different resisting components and the minimum embedment length criteria, are evaluated and discussed. The results showed that the maximum and minimum differences between the 3D FE modeling and one-dimensional (1D) DNV p–y method are 41% and 11% for large displacements, and 32.5% and 13.3% for small displacements, respectively. As the slenderness ratio increases, the discrepancy between the finite element (FE) modeling results and the 1D DNV p–y method decreases, with an average difference of about 13% across all monopile diameters at an L/D ratio of 10, in both small and large displacements, indicating the reasonable accuracy of the 1D method for slenderness ratios of 10 and above. Among the three minimum embedment length criteria examined, the DNV recommended and vertical-tangent criteria offered shorter embedment lengths. The primary resisting moment across all slenderness ratios comes from the distributed lateral load along the monopile shaft (${\mathrm{M}\mathrm{C}\mathrm{R}}_{\mathrm{p}-\mathrm{y}}$), which increases as the L/D ratio increases. Full article

Background/Objectives: Immune effector cell-associated hematotoxicity (ICAHT) is a frequent adverse event after chimeric antigen receptor (CAR)-T cell therapy. Grade ≥ 3 thrombocytopenia occurs in around one-third of patients, and many of them become platelet transfusion-dependent. Eltrombopag is a thrombopoietin receptor agonist (TPO-RA) able to accelerate megakaryopoiesis, which has been used successfully in patients with bone marrow failure and immune thrombocytopenia (ITP). Its role in managing thrombocytopenia and other cytopenias in CAR-T cell-treated patients has been scarcely addressed. Our aim was to report the safety and efficacy of this approach in patients included in the Spanish Group for Hematopoietic Transplantation and Cellular Therapy (GETH-TC) registry. Methods: This is a retrospective, multicenter, observational study. Patients who developed platelet transfusion dependence subsequently to CAR-T cells and received eltrombopag to improve platelet counts were recruited in 10 Spanish hospitals. Results: Thirty-eight patients were enrolled and followed up for a median (interquartile range [IQR]) of 175 (99, 489) days since CAR-T cell infusion. At the moment eltrombopag was indicated, 18 patients had thrombocytopenia and another severe cytopenia, while 8 patients had severe pancytopenia. After 32 (14, 38) days on eltrombopag, 29 (76.3%) patients recovered platelet transfusion independence. The number of platelet units transfused correlated with the time needed to restore platelet counts higher than 20 × 10⁹/L (Rho = 0.639, p < 0.001). Non-responders to eltrombopag required more platelet units (58 [29, 69] vs. 12 [6, 26] in responders, p = 0.002). Nineteen out of twenty-three (82.6%) patients recovered from severe neutropenia after 22 (11, 31) days on eltrombopag. Twenty-nine out of thirty-five (82.9%) patients recovered red blood cell (RBC) transfusion independence after 29 (17, 44) days. Seven patients recovered all cell lineages while on treatment. No thromboembolic events were reported. Only two transient toxicities (cholestasis, hyperbilirubinemia) were reported during eltrombopag treatment, none of which compelled permanent drug withdrawal. Conclusions: Eltrombopag could be safely used to manage thrombocytopenia and accelerate transfusion independence in CAR-T cell-treated patients. Full article

Background: Physiological curvature changes of the lumbar spine and disc herniation can cause abnormal biomechanical responses of the lumbar spine. Finite element (FE) studies on special weightlifter models are limited, yet understanding stress in damaged lumbar spines is crucial for preventing and rehabilitating lumbar diseases. This study analyzes the biomechanical responses of a weightlifter with lumbar straightening and L4-L5 disc herniation during symmetric bending and lifting to optimize training and rehabilitation. Methods: Based on the weightlifter’s computed tomography (CT) data, an FE lumbar spine model (L1-L5) was established. The model included normal intervertebral discs (IVDs), vertebral endplates, ligaments, and a degenerated L4-L5 disc. The bending angle was set to 45°, and weights of 15 kg, 20 kg, and 25 kg were used. The flexion moment for lifting these weights was theoretically calculated. The model was tilted at 45° in Abaqus 2021 (Dassault Systèmes Simulia Corp., Johnston, RI, USA), with L5 constrained in all six degrees of freedom. A vertical load equivalent to the weightlifter’s body mass and the calculated flexion moments were applied to L1 to simulate the weightlifter’s bending and lifting behavior. Biomechanical responses within the lumbar spine were then analyzed. Results: The displacement and range of motion (ROM) of the lumbar spine were similar under all three loading conditions. The flexion degree increased with the load, while extension remained unchanged. Right-side movement and bending showed minimal change, with slightly more right rotation. Stress distribution trends were similar across loads, primarily concentrated in the vertebral body, increasing with load. Maximum stress occurred at the anterior inferior margin of L5, with significant stress at the posterior joints, ligaments, and spinous processes. The posterior L5 and margins of L1 and L5 experienced high stress. The degenerated L4-L5 IVD showed stress concentration on its edges, with significant stress also on L3-L4 IVD. Stress distribution in the lumbar spine was uneven. Conclusions: Our findings highlight the impact on spinal biomechanics and suggest reducing anisotropic loading and being cautious of loaded flexion positions affecting posterior joints, IVDs, and vertebrae. This study offers valuable insights for the rehabilitation and treatment of similar patients. Full article

Kokusaginine is a bioactive ingredient extracted from Ruta graveolens L., which has a range of biological activities. Its pharmacokinetic (PK) properties are particularly important for clinical applications; however, they have not been fully elucidated. In addition, the effect of sex differences on drug metabolism is increasingly being recognized, but most studies have ignored this important factor. This study aims to fill this knowledge gap by taking an in-depth look at the PK properties of kokusaginine and how gender affects its metabolism and distribution in the body. It also lays the foundation for clinical drug development. In this study, a sensitive ultra-high-performance liquid chromatography (UPLC) method was developed and validated for quantifying kokusaginine in Sprague Dawley (SD) rat plasma and tissue homogenates. Metabolic stability was evaluated in vitro using gender-specific liver microsomes. Innovatively, we incorporated sex as a variable into both in vitro and in vivo PK studies in SD rats, analyzing key parameters with Phoenix 8.3.5 software. The developed UPLC method demonstrated high sensitivity and precision, essential for PK analysis. Notably, in vitro studies revealed a pronounced sex-dependent metabolic variability (p < 0.05). In vivo, gender significantly affected the Area Under the Moment Curve (AUMC)_(0-∞) of the plasma PK parameter (p < 0.05) and the AUMC_(0-t) of brain tissue (p < 0.0001), underscoring the necessity of sex-specific PK assessments. The calculated absolute bioavailability of 71.13 ± 12.75% confirmed the favorable oral absorption of kokusaginine. Additionally, our innovative tissue-plasma partition coefficient (K_p) analysis highlighted a rapid and uniform tissue distribution pattern. This study presents a sex-inclusive PK evaluation of kokusaginine, offering novel insights into its metabolic profile and distribution. These findings are instrumental for informing clinical medication practices, dosage optimization, and a nuanced understanding of drug efficacy and safety in a sex-specific context. Full article

The manuscript presents the applicability of the Gumbel distribution in the frequency analysis of extreme events in hydrology. The advantages and disadvantages of using the distribution are highlighted, as well as recommendations regarding its proper use. A literature review was also carried out regarding the methods for estimating the parameters of the Gumbel distribution in hydrology. Thus, for the verification of the methods, case studies are presented regarding the determination of the maximum annual flows and precipitations using nine methods for estimating the distribution parameters. The influence of the variability of the observed data lengths on the estimation of the statistical indicators, the estimation of the parameters, and the quantiles corresponding to the field of small exceedance probabilities (p < 1%) is also highlighted. In each case, the results are analyzed compared to those obtained with the Generalized Extreme Value distribution, the four-parameter Burr distribution, and the five-parameter Wakeby distribution estimated using the L-moments method. The results of the case studies highlight and reaffirm the statistical, mathematical, and hydrological recommendations regarding the avoidance of applying the Gumbel distribution in flood frequency analysis and its use with reservations in the case of maximum precipitation analysis, especially when the statistical indicators of the analyzed data are not close to the characteristic ones and unique to the distribution. Full article

Effective forest management is essential for mitigating climate change effects. This is why understanding forest growth dynamics is critical for its sustainable management. Thus, characterizing forest plot deadwood levels is vital for understanding forest dynamics, and for assessments of biomass, carbon stock, and biodiversity. For the first time, this study used the leaf area index (LAI) and L-moments to characterize and model forest plot deadwood levels in the Bavarian Forest National Park from airborne laser scanning (ALS) data. This study proposes methods that can be tested for forests, especially those in temperate climates with frequent cloud coverage and limited access. The proposed method is practically significant for effective planning and management of forest resources. First, plot decay levels were characterized based on their canopy leaf area density (LAD). Then, the deadwood levels were modeled to assess the relationships between the vegetation area index (VAI), gap fraction (GF), and the third L-moment ratio (T3). Finally, we tested the rule-based methods for classifying plot decay levels based on their biophysical structures. Our results per the LAD vertical profiles clearly showed the declining levels of decay from Level 1 to 5. Our findings from the models indicate that at a 95% confidence interval, 96% of the variation in GF was explained by the VAI with a significant negative association (VAIslope = −0.047; R² = 0.96; (p < 0.001)), while the VAI explained 92% of the variation in T3 with a significant negative association (VAIslope = −0.50; R² = 0.92; (p < 0.001)). Testing the rule-based methods, we found that the first rule (Lcv = 0.5) classified Levels 1 and 2 at (Lcv < 0.5) against Levels 3 to 5 at (Lcv > 0.5). However, the second rule (Lskew = 0) classified Level 1 (healthy plots) as closed canopy areas (Lskew < 0) against Levels 2 to 5 (deadwood) as open canopy areas (Lskew > 0). This approach is simple and more convenient for forest managers to exploit for mapping large forest gap areas for planning and managing forest resources for improved and effective forest management. Full article

Extensive research has been conducted on the in vitro mass propagation of pear (Pyrus spp.) trees through vegetative propagation, demonstrating high efficiency in shoot multiplication across various pear species. However, the low in vitro rooting rates remain a significant barrier to the practical application and commercialization of mass propagation. This study aims to determine the favorable conditions for inducing root formation in the in vitro microshoots of Pyrus genotypes. The base of the microshoots was exposed to a high concentration (2 mg L⁻¹) of auxins (a combination of IBA and NAA) for initial root induction at the moment when callus formation begins. The microshoots were then transferred to an R1 medium (1/2 MS with 30 g L⁻¹ sucrose without PGRs) to promote root development. This method successfully induced rooting in three European pear varieties, one Asian pear variety, and a European–Asian hybrid, resulting in rooting rates of 66.7%, 87.2%, and 100% for the European pear (P. communis), 60% for the Asian pear (P. pyrifolia), and 83.3% for the hybrid pear (P. pyrifolia × P. communis) with an average of 25 days. In contrast, the control group (MS medium) exhibited rooting rates of 0–13.3% after 60 days of culture. These findings will enhance in vitro root induction for various pear varieties and support the mass propagation and acclimatization of pear. The in vitro root induction method developed in this study has the potential for global commercial application in pear cultivation. Full article

This study investigates the effect of pure yeast culture fermentation versus spontaneous fermentation on the volatile compound profile of industrially produced plum brandy. Using traditional distillation methods, the evolution of key volatile compounds is monitored at seven different moments during the distillation process. By integrating advanced analytical techniques such as GC-MS and sensory evaluation, significant differences in the composition of the distillates are highlighted, particularly in terms of ethyl esters and higher alcohols which are key to the sensory properties of the final product. Distillates produced with the addition of pure cultures gave higher concentrations of esters than those obtained by wild fermentation. The results of our industrial research show that the most critical step is to limit the storage of the input raw material, thereby reducing the subsequent risk of producing higher concentrations of 1-propanol. Furthermore, our results indicate that the heart of the distillate can only be removed up to an ethanol content of approximately 450 g/L and that the removal of additional ethanol results in only a 10% increase in the total volume of the distillate, which in turn results in an increase in boiler heating costs of approximately 30%. Full article

Steel–concrete composite structures have advantages in terms of strong bearing capacity and full utilisation of performance, and thus, composite frame beams are widely used in building construction. However, in the design and use of existing composite frame beams, the composite effect of a slab and steel beam cannot be completely taken into account. In this study, the effective flange width method is utilised to calculate the contribution of the slab reinforcement to the section moment of inertia to check the beam-end crack width via simulations using the general finite-element software MSC.MARC 2020. A parameter sensitivity analysis of the reinforcement tensile stress is conducted to determine critical influential geometric parameters for the side-column and centre-column hogging moment regions. Finally, design formulae for calculating the effective flange widths of the side- and centre-column hogging moment regions are proposed. In the formula for the side-column hogging moment region, the half column width ($R$) and steel-beam height ($h_{\mathrm{s}}$) are critical variables, whereas, in the formula for the centre-column hogging moment region, the steel-beam height ($h_{\mathrm{s}}$), slab width ($b_{\mathrm{c}}$), and half clear-span length ($l$) are critical variables. Both formulas are verified via a multiparameter simulation, which enables more accurate crack-checking calculations for the hogging moment region in the serviceability limit state. This study provides an important reference for fine finite-element simulations of serviceability limit states and shows the factors affecting the effective flange width that differ from those in the ultimate limit state. Full article

Sperm capacitation involves biochemical and physiological changes that enable sperm to fertilize the oocyte. It can be induced in vitro under controlled conditions that simulate the environment of the oviduct. While extensively studied in mammals, its approach in lizards remains absent. Understanding the mechanisms that ensure reproduction is essential for advancing the implementation of assisted reproductive technologies in this group. We aimed to perform a sperm analysis to determine if capacitation-related changes were induced after incubation with capacitating media. Fifteen males of Sceloporus torquatus were collected during the early stage of the reproductive season. The sperm were isolated from the seminal plasma and then diluted up to a volume of 150 μL using BWW medium to incubate with 5% CO₂ at 30 °C for a maximum duration of 3 h. A fraction was retrieved hourly for ongoing sperm assessment. The sperm analysis included assessments of its motility, viability, the capacitation status using the chlortetracycline (CTC) assay, and the acrosome integrity with the lectin binding assay to detect changes during incubation. We found that total motility was maintained up to 2 h post incubation, after which it decreased. However, sperm viability remained constant. From that moment on, we observed a transition to a deeper and less symmetrical flagellar bending in many spermatozoa. The CTC assay indicated a reduction in the percentage of sperm showing the full (F) pattern and an increase in those exhibiting the capacitated (B) and reactive (RA) patterns, accompanied by an elevation in the percentage of damaged acrosomes as revealed by the lectin binding assay. In mammals, these changes are often associated with sperm capacitation. Our observations support the notion that this process may also occur in saurian. While sperm analysis is a valuable method for assessing certain functional changes, additional approaches are required to validate this process. Full article

Background: The COVID-19 lockdown has been a major stressor for the general population, posing a considerable threat to quality of life (QoL), particularly among university students. Existing research highlights the protective role of dispositional mindfulness (DM) in mitigating stressors; however, its influence on moderating the impact of COVID-19 on QoL remains unknown. We used a longitudinal design to assess the QoL of undergraduate students before and after the COVID-19 lockdown, while also examining the potential moderating effect of DM on this impact. Methods: One hundred eleven Spanish undergraduate students were recruited in 2019, and 103 were followed-up in 2020. Instruments comprised a demographic questionnaire, the World Health Organization Quality of Life BREF (WHOQOL-BREF) inventory to assess QoL, and the Five Facets Mindfulness Questionnaire (FFMQ) to assess DM. Results: Analyses revealed statistically significant differences between the two time points in WHOQOL-BREF: Psychological, Social Relationships, and Environmental. Moderation analyses revealed that the impact of COVID-19 on WHOQOL-BREF Psychological scores was moderated by FFMQ-Observe and FFMQ-Non-judging. Conclusions: The COVID-19 lockdown resulted in a reduction of QoL among undergraduate students, yet this impact was moderated by DM. Specifically, present moment attention to experience (observe) and non-judgmental awareness attenuated the impact of COVID-19 on psychological well-being. Future research should focus on evaluating the protective role of preventive interventions designed to increase DM among undergraduate students. Full article

This paper presents an application of L-moments and respective L-moment ratio diagrams (LMRD) to the task of control performance assessment (CPA). An L-moment ratio diagram is a graphical approach to the visualization of statistical properties for a given time series. Moreover, it enables comparing various data, showing their similarities and homogeneity. Simultaneously, CPA aims at measuring the control loop quality, supporting decision-making about their tuning and maintenance. This paper shows that control system quality can be efficiently visualized using LMRDs. The method was analyzed using simulations and further validated at a real chemical engineering industrial ammonia synthesis plant. Full article

Background: Cardiorespiratory fitness (CRF) is an important component of overall physical fitness and is associated with numerous health benefits, including a reduced risk of heart disease, diabetes, and obesity. However, direct measurement of CRF is time-consuming and therefore not feasible for screening purposes. Methods: A maximal treadmill exercise test with the Bruce protocol was performed to estimate VO_2max in 1047 Spanish men and women aged 17 to 62 years. Weight, height, and heart rate were measured. Leisure-time physical activity (LTPA) was recorded using the Minnesota Leisure Time Physical Activity Questionnaire. A multiple linear regression model was developed to predict exercise-based VO_2max. The validity of the model was examined by correlation, concordance, Bland–Altman analysis, cross-validation, and construct validity analysis. Results: There was no significant difference between VO_2max obtained by the Bruce protocol (43.56 mL/kg/min) or predicted by the equation (43.59 mL/kg/min), with R² of 0.57, and a standard error of the estimate of 7.59 mL/kg/min. Pearson’s product–moment correlation and Lin’s concordance correlation between measured and predicted CRF values were 0.75 and 0.72, respectively. Bland–Altman analysis revealed a significant proportional bias of non-exercise eCRF, overestimating unfit and underestimating highly fit individuals. However, 64.3% of participants were correctly classified into CRF tertile categories, with an important 69.9% in the unfit category. Conclusions: The eCRF equation was associated with several cardiovascular risk factors in the anticipated directions, indicating good construct validity. In conclusion, the non-exercise eCRF showed a reasonable validity to estimate true VO_2max, and it may be a useful tool for screening CRF. Full article