Search Results (289)

Within the phylum Cnidaria, sea anemones (class Anthozoa) express a rich diversity of ion-channel peptide modulators with biomedical applications, but corollary discoveries from jellyfish (subphylum Medusozoa) are lacking. To bridge this gap, bioactivities of previously unexplored proteinaceous and small molecular weight (~15 kDa to 5 kDa) venom components were assessed in a mouse dorsal root ganglia (DRG) high-content calcium-imaging assay, known as constellation pharmacology. While the addition of crude venom led to nonspecific cell death and Fura-2 signal leakage due to pore-forming activity, purified small molecular weight fractions of venom demonstrated three main, concentration-dependent and reversible effects on defined heterogeneous cell types found in the primary cultures of mouse DRG. These three phenotypic responses are herein referred to as phenotype A, B and C: excitatory amplification (A) or inhibition (B) of KCl-induced calcium signals, and test compound-induced disturbances to baseline calcium levels (C). Most notably, certain Alatina alata venom fractions showed phenotype A effects in all DRG neurons; Physalia physalis and Chironex fleckeri fractions predominantly showed phenotype B effects in small- and medium-diameter neurons. Finally, specific Physalia physalis and Alatina alata venom components induced direct excitatory responses (phenotype C) in glial cells. These findings demonstrate a diversity of neuroactive compounds in jellyfish venom potentially targeting a constellation of ion channels and ligand-gated receptors with broad physiological implications. Full article

Precise water level forecasting plays a decisive role in improving the efficiency of flood prevention and disaster reduction, optimizing water resource management, enhancing the safety of waterway transportation, reducing flood risks, and promoting ecological and environmental protection, which is crucial for the sustainable development of society. This study proposes a hybrid water level forecasting model based on Time-Varying Filter-based Empirical Mode Decomposition (TVFEMD), Inverse-Free Extreme Learning Machine (IFELM), and error correction. Firstly, historical water level data are decomposed into different modes using TVFEMD; secondly, the Improved Jellyfish Search (IJS) algorithm is employed to optimize the IFELM, and subsequently, the optimized IFELM independently forecasts each sub-sequence and obtains the predictive results of each sub-sequence; thirdly, an Online Sequential Extreme Learning Machine (OSELM) model is used to correct data errors, and the initial predictive results and error prediction results are added together to obtain the final prediction for the sub-sequence; and finally, the final prediction for the sub-sequences are added to obtain the prediction results of the entire water level sequence. Taking the daily water level data from 2006 to 2018 in Taihu, China as the research object, this paper compares the proposed model with the ELM, BP, LSTM, IFELM, TVFEMD-IFELM, and TVFEMD-IFELM-OSELM models. The results show that the TVFEMD-IJS-IFELM-OSELM model established in this study has high prediction accuracy and strong stability and is suitable for water level forecasting. Full article

This study was carried out to elucidate the causes of massive outbreaks of Aurelia coerulea in Geoje Bay, Korea, from November 2022 to October 2023. Adult medusae consistently spawn with planulae, and the populations of A. coerulea in Geoje Bay could be categorized into current-year and overwintering populations. The current-year population began with the emergence of ephyrae in February and grew until October, while the overwintering population comprised a mixture of surviving current-year population and additional individuals that joined during the warm season. The size of the planulae are significantly larger than the annual average during the cold season. These results appear to be the energy accumulation of planulae for polyp formation under low water temperatures. Planulae form polyps within a temperature range of 5–25 °C, suggesting the possibility of year-round polyp recruitment. In Geoje Bay, the highest appearance rate of A. coerulea was in April (8.71 ± 12.5 ind. m⁻³), with ephyrae experiencing higher growth rates up to the young medusa stage. However, from April, a decline in zooplankton biomass resulted in reduced growth rates in adults, indicating that jellyfish growth was primarily regulated by food availability. Additionally, submersed oyster shells in oyster farms served as the main habitat for jellyfish polyps. A. coerulea populations were also characterized by the continuous spawning of planulae throughout the year. In conclusion, this study suggests that stable polyp habitats, abundant food supply during the initial developmental period of the population, and suitable ranges of water temperature were significant factors inducing the massive outbreak of A. coerulea in Geoje Bay, Korea. Full article

This paper presents a new optimization technique based on the hybridization of two meta-heuristic methods, Jellyfish Search (JS) and Moth Flame Optimizer (MFO), to solve the Optimal Power Flow (OPF) problem. The JS algorithm offers good exploration capacity but lacks performance in its exploitation mechanism. To improve its efficiency, we combined it with the Moth Flame Optimizer, which has proven its ability to exploit good solutions in the search area. This hybrid algorithm combines the advantages of both algorithms. The performance and precision of the hybrid optimization approach (JS-MFO) were investigated by minimizing well-known mathematical benchmark functions and by solving the complex OPF problem. The OPF problem was solved by optimizing non-convex objective functions such as total fuel cost, total active transmission losses, total gas emission, total voltage deviation, and the voltage stability index. Two test systems, the IEEE 30-bus network and the Mauritanian RIM 27-bus transmission network, were considered for implementing the JS-MFO approach. Experimental tests of the JS, MFO, and JS-MFO algorithms on eight well-known benchmark functions, the IEEE 30-bus, and the Mauritanian RIM 27-bus system were conducted. For the IEEE 30-bus test system, the proposed hybrid approach provides a percent cost saving of 11.4028%, a percent gas emission reduction of 14.38%, and a percent loss saving of 50.60% with respect to the base case. For the RIM 27-bus system, JS-MFO achieved a loss percent saving of 50.67% and percent voltage reduction of 62.44% with reference to the base case. The simulation results using JS-MFO and obtained with the MATLAB 2009b software were compared with those of JS, MFO, and other well-known meta-heuristics cited in the literature. The comparison report proves the superiority of the JS-MFO method over JS, MFO, and other competing meta-heuristics in solving difficult OPF problems. Full article

Recently, jellyfish venom has gained attention as a promising reservoir of pharmacologically active compounds, with potential applications in new drug development. In this investigation, novel peptides, isolated from the hydrolysates of Nemopilema nomurai jellyfish venom (NnV), demonstrate potent inhibitory activities against angiotensin-converting enzyme (ACE). Proteolytic enzymes—specifically, papain and protamex—were utilized for the hydrolysis under optimized enzymatic conditions, determined by assessing the degree of hydrolysis through the ninhydrin test. Comparative analyses revealed that papain treatment exhibited a notably higher degree of NnV hydrolysis compared to protamex treatment. ACE inhibitory activity was quantified using ACE kit-WST, indicating a substantial inhibitory effect of 76.31% for the papain-digested NnV crude hydrolysate, which was validated by captopril as a positive control. The separation of the NnV-hydrolysate using DEAE sepharose weak-anion-exchange chromatography revealed nine peaks under a 0–1 M NaCl stepwise gradient, with peak no. 3 displaying the highest ACE inhibition of 96%. The further purification of peak no. 3 through ODS-C18 column reverse-phase high-performance liquid chromatography resulted in five sub-peaks (3.1, 3.2, 3.3, 3.4, and 3.5), among which 3.2 exhibited the most significant inhibitory activity of 95.74%. The subsequent analysis of the active peak (3.2) using MALDI–TOF/MS identified two peptides with distinct molecular weights of 896.48 and 1227.651. The peptide sequence determined by MS/MS analysis revealed them as IVGRPLANG and IGDEPRHQYL. The docking studies of the two ACE-inhibitory peptides for ACE molecule demonstrated a binding affinity of −51.4 ± 2.5 and −62.3 ± 3.3 using the HADDOCK scoring function. Full article

Owing to global climate change or the ever-more frequent human activities in the offshore areas, it is highly probable that an imbalance in the offshore ecosystem has been induced. However, the importance of maintaining and protecting marine ecosystems’ balance cannot be overstated. In recent years, various marine disasters have occurred frequently, such as harmful algal blooms (green tides and red tides), storm surge disasters, wave disasters, sea ice disasters, and tsunami disasters. Additionally, overpopulation of certain marine organisms (particularly marine faunas) has led to marine disasters, threatening both marine ecosystems and human safety. The marine ecological disaster monitoring system in China primarily focuses on monitoring and controlling the outbreak of green tides (mainly caused by outbreaks of some Ulva species) and red tides (mainly caused by outbreaks of some diatom and dinoflagellate species). Currently, there are outbreaks of Cnidaria (Hydrozoa and Scyphozoa organisms; outbreak species are frequently referred to as jellyfish), Annelida (Urechis unicinctus Drasche, 1880), Mollusca (Philine kinglipini S. Tchang, 1934), Arthropoda (Acetes chinensis Hansen, 1919), and Echinodermata (Asteroidea organisms, Ophiuroidea organisms, and Acaudina molpadioides Semper, 1867) in China. They not only cause significant damage to marine fisheries, tourism, coastal industries, and ship navigation but also have profound impacts on marine ecosystems, especially near nuclear power plants, sea bathing beaches, and infrastructures, posing threats to human lives. Therefore, this review provides a detailed introduction to the marine organisms (especially marine fauna species) causing marine biological disasters in China, the current outbreak situations, and the biological backgrounds of these outbreaks. This review also provides an analysis of the causes of these outbreaks. Furthermore, it presents future prospects for marine biological disasters, proposing corresponding measures and advocating for enhanced resource utilization and fundamental research. It is recommended that future efforts focus on improving the monitoring of marine biological disasters and integrating them into the marine ecological disaster monitoring system. The aim of this review is to offer reference information and constructive suggestions for enhancing future monitoring, early warning systems, and prevention efforts related to marine ecological disasters in support of the healthy development and stable operation of marine ecosystems. Full article

In this study, acoustic, sighting, trawl, and marine environmental surveys were used to determine the vertical distribution and density of giant jellyfish that have been observed in Korean waters over the past 3 years. From 2020 to 2022, annual surveys were conducted in May and July in the East China Sea and waters adjacent to South Korea. The acoustic data were processed by identifying and eliminating all signals considered as noise while excluding those suspected to be jellyfish signals. Subsequently, a single target detection method was employed. Giant jellyfish are distributed mostly in the middle and low layers. In May 2021, the average population density of giant jellyfish was recorded as 11.6 (ind./ha), which was the highest density. In July 2022, this value decreased to 1.7 (ind./ha), marking the lowest density. The sighting survey, which allows for the identification of jellyfish distributed in the surface layer, exhibited a difference of approximately 0.13 times compared to the acoustic survey conducted in the middle and low layers in 2020. In 2021 and 2022, this difference was approximately 0.11 times and 0.24 times, respectively. The average of this difference was 0.16 times or greater. Full article

The giant jellyfish Nemopilema nomurai sting can cause local and systemic reactions; however, comparative analysis of the tentacle extract (TE) and nematocyst venom extract (NV), and its toxicity, mechanism, and potential intervention are still limited. This study compared venom from TE and NV for their composition, toxicity, and efficacy in vitro and in vivo used RAW264.7 cells and ICR mice. A total of 239 and 225 toxin proteins were identified in TE and NV by proteomics, respectively. Pathological analysis revealed that TE and NV caused heart and liver damage through apoptosis, necrosis, and inflammation, while TE exhibited higher toxicity ex vivo and in vivo. Biochemical markers indicated TE and NV elevated creatine kinase, lactatedehydrogenase, and aspartate aminotransferase, with the TE group showing a more significant increase. Transcriptomics and Western blotting indicated both venoms increased cytokines expression and MAPK signaling pathways. Additionally, 1 mg/kg PACOCF3 (the phospholipase A2 inhibitor) improved survival from 16.7% to 75% in mice. Our results indicate that different extraction methods impact venom activities, tentacle autolysis preserves toxin proteins and their toxicity, and PACOCF3 is a potential antidote, which establishes a good extraction method of jellyfish venom, expands our understanding of jellyfish toxicity, mechanism, and provides a promising intervention. Full article

Atherosclerosis, a noncommunicable disease caused by cholesterol plaque, can cause chronic diseases. The antiplatelet medicines used in its treatment can cause complications. Marine collagen peptides can be used as a natural atherosclerosis remedy. The present study investigated the preparation and characterization of hydrolyzed collagen (HC) from jellyfish and its conjugation with black jelly mushroom extract (BJME). Their cytotoxicity and ability to prevent cholesterol-induced endothelial cell injury were also examined. HC was prepared using Alcalase or papain hydrolysis (0.2–0.4 units/g of dry matter (DM)). Higher yield, degree of hydrolysis, and antioxidant activities (AAs) were found in the HC obtained from Alcalase, especially at 0.4 units/g DM (A-0.4), compared to other processes (p < 0.05). Thus, A-0.4 was further conjugated with BJME (1–4%, w/w of HC). The HC-2%BJME conjugate showed the highest surface hydrophobicity and AAs compared to other samples. The FTIR spectra and size distribution also confirmed the conjugation between HC and BJME. When EA.hy926 endothelial cells were treated with HC or HC-2%BJME (25–1000 µg/mL), HC-2%BJME had no cytotoxicity, whereas HC at 1000 µg/mL induced cytotoxicity (p < 0.05). Both samples also exhibited protective ability against cholesterol-induced apoptosis and VE-cadherin downregulation of cells. Therefore, HC and conjugate could be natural agents for preventing atherosclerosis. Full article

Collagen peptides and marine collagen are enormous resources currently utilized. This review aims to examine the scientific literature to determine which collagen peptides derived from marine sources and which natural active antioxidants from marine collagen have significant biological effects as health-promoting nutraceuticals. Marine collagen is extracted from both vertebrate and invertebrate marine creatures. For vertebrates, this includes fish skin, bones, scales, fins, and cartilage. For invertebrates, it includes mollusks, echinoderms, crustaceans, and poriferans. The method used involved data analysis to organize information for isolating and identifying marine biocompounds with antioxidant properties. Specifically, amino acids with antioxidant properties were identified, enabling the use of hydrolysates and collagen peptides as natural antioxidant nutraceuticals. The methods of extraction of hydrolyzed collagen and collagen peptides by different treatments are systematized. The structural characteristics of collagen, collagen peptides, and amino acids in fish skin and by-products, as well as in invertebrate organisms (jellyfish, mollusks, and crustaceans), are described. The antioxidant properties of different methods of collagen hydrolysates and collagen peptides are systematized, and the results are comparatively analyzed. Their use as natural antioxidant nutraceuticals expands the range of possibilities for the exploitation of natural resources that have not been widely used until now. Full article

The relative lack of marine venom could be attributed to the difficulty in dealing with venomous marine animals. Moreover, the venom of marine animals consists of various bioactive molecules, many of which are proteins with unique properties. In this study, we investigated the potential toxic proteins of jellyfish collected for ligand screening to understand the mechanism of the toxic effects of jellyfish. Since taxonomic identification is problematic due to the lack of proper keys, we conducted morphological and molecular mitochondrial DNA sequencing from COI and ITS regions. The venom extract from nematocysts found along the bell margins was used for protein characterization using SDS-gel electrophoresis and nano-liquid chromatography-tandem mass spectrometry. Ligand screening for the most potent toxin and antibacterial and cytotoxicity assays were carried out. The phylogenetic tree showed distinct clustering from other Catostylus sp. The proteomic analysis revealed venom with many bioactive proteins. Only 13 venom proteins were identified with molecular weights ranging from 4318 to 184,923 Da, exhibiting the venom’s complexity. The overall toxin protein composition of Catostylus sp. venom was dominated by potassium channel toxin alpha-KTx. Molecular docking of toxin alpha-KTx 1.13 revealed high specificity towards the human voltage-gated potassium channel Kv3 with a high fitness score and a minimum energy barrier of −17.9 kcal/mol. Disc diffusion and cytotoxicity assays revealed potent antibacterial activity against Klebsiella pneumoniae with no cytotoxicity. Further studies on detailed characterization and therapeutic potentials are warranted. Full article

The family Eirenidae is one of the major taxa of the order Leptothecata, comprising approximately 80 species from ten genera. In this study, taxonomic investigations, including morphological observations and molecular 16S phylogenetic analyses, were conducted on unknown Eirenidae specimens collected off the coast of Iwaki, Fukushima Prefecture, eastern Japan, in June 2022. The specimens had the following morphological characteristics: marginal warts and tentacular bulbs with lateral cirri and without adaxial papillae, a mouth with simple lips, four simple radial canals, and eight statocysts common to the genus Eutima. However, this species can be distinguished from other species of Eutima by the number of tentacles, number and shape of marginal warts, position of the gonads, and gastric peduncle length. Moreover, the monophyly of the species was evident in the 16S rRNA phylogenetic tree (as indicated by the high bootstrap value of 100%), thereby supporting the validity of the new species. Based on these results, we describe it as a new species, Eutima onahamaensis, for taxonomic stabilization. We also made detailed observations of the morphology and molecular phylogenetic analyses of one of the species newly recorded in Japan: Eutima diademata. A comparative table of the primary diagnostic characteristics of Eutima has been provided. This study provided taxonomic keys for identifying species in the genus Eutima. Full article

Envenomation by marine animals poses a significant health concern globally, affecting both local residents and tourists in coastal regions. The primary objective of this review is to critically evaluate the existing scientific literature to determine the most effective first-aid treatment for envenomations caused by marine animals, specifically whether hot-water immersion (HWI) or ice-pack treatment (IPT) provides the best immediate care. This comprehensive review covers a wide range of marine envenomations, from jellyfish stings to stingray injuries. While our focus is primarily on the efficacy of HWI and IPT, we also explore the role of cold-water treatment as a result of its relevance and similarity to ice-pack applications. In addition, we examine other treatments mentioned in the literature, such as medications or vinegar, and highlight their findings where applicable. To provide a clear and structured overview, we summarised the articles in separate tables. These tables categorise the type of research conducted, the marine species studied, the region of origin of the marine species, and the key findings of each study. Our analysis of the available evidence indicates a general consensus in the scientific community on the effectiveness of HWI or IPT for envenomation by marine animals. However, when treating those injuries, it is crucial to consider all factors since there is no universally superior treatment due to the diverse nature of marine habitats. Full article

Although jellyfish represent a food source in Asia, limited attention has been devoted to investigating Western consumers’ perception and acceptance. This study explored the role of jellyfish body parts and presentation form in determining consumer perception. A local consumer test with 106 untrained subjects (57.5% female, 18–45 years) was performed in Italy over two days on six samples of jellyfish (Rhopilema esculentum Kishinouye) differing in terms of body parts (umbrella and oral arms) and presentation form (minced, striped, and pieced). For each sample, participants expressed their overall liking and, through three check-all-that-apply tests, described their perceived sensory properties and emotions and potential preferred food pairings. The results showed a significant effect of presentation form on liking (with striped and minced samples liked more than pieced samples), 18 sensory properties, four emotions, and five food pairings. Moreover, different drivers of liking and emotions were observed for three clusters of subjects named “In favour of”, “Against”, and “Picky towards” eating jellyfish. In conclusion, this study found that at least one segment of consumers could accept jellyfish as novel food. Moreover, the provided results could be useful for developing innovative jellyfish-based products and dishes that meet consumers’ expectations. Full article

In recent years, there have been frequent jellyfish outbreaks in Chinese coastal waters, significantly impacting the structure, functionality, safety, and economy of nuclear power plant cooling water intake and nearby ecosystems. Therefore, this study focuses on jellyfish outbreaks in Chinese coastal waters, particularly near the Shandong Peninsula. By analyzing jellyfish abundance data, a Generalized Additive Model integrating environmental factors reveals that temperature and salinity greatly influence jellyfish density. The results show variations in jellyfish density among years, with higher densities in coastal areas. The model explains 42.2% of the variance, highlighting the positive correlation between temperature (20–26 °C) and jellyfish density, as well as the impact of salinity (27.5–29‰). Additionally, ocean currents play a significant role in nearshore jellyfish aggregation, with a correlation between ocean currents and site coordinates. This study aims to investigate the relationship between jellyfish blooms and environmental factors. The results obtained from the study provide data support for the prevention and control of blockages in nuclear power plant cooling systems, and provide a data basis for the implementation of monitoring measures in nuclear power plants. Full article