Search Results (40)

Poultry bio-mapping helps identify microbial contamination and process optimization opportunities such as sanitary dressing procedures, equipment adjustments, cross-contamination controls, and chemical intervention applications in commercial poultry processing operations. This study focuses on the development of a preliminary microbiological baseline of microbial indicators, including total viable counts (TVC), Enterobacteriaceae (EB), and the quantification and detection of pathogens such as Salmonella and Campylobacter spp. in four commercial broiler processing facilities in Honduras. Whole chicken and wing rinses were collected from four poultry processing plants at different locations: live receiving, rehanger, post-evisceration, post-chiller, and wings. The MicroSnap^® system was used for the enumeration of microbial indicators, the BAX^®-System-SalQuant^® was used for the quantification of Salmonella, and the BAX^®-System-CampyQuant™ was used for Campylobacter spp. Negative samples after enumeration were tested with BAX^®-System Salmonella and BAX^®-System Campylobacter for prevalence analysis, respectively. The TVC and EB counts were continuously reduced from the live receiving to the post-chiller location, presenting a statistically significant increase (p < 0.01) at the wings location. The Salmonella counts were significantly different between stages (p < 0.01). The prevalence of Salmonella was highest in the live receiving stage, with 92.50%, while that at the post-chiller stage was the lowest, at 15.38%. Campylobacter spp. counts were lower than that at the other stages at wings 1.61 Log CFU/sample; however, Campylobacter spp.’s prevalence was higher than 62.5% in all stages. Microbial bio-mapping using novel technologies suitable for mobile applications was conducted in this study to establish statistical process control parameters for microorganisms. A nationwide microbial baseline for commercial broiler processing facilities in Honduras was developed. In-country data serve as a benchmark for continuous improvement at each facility evaluated and can assist regulatory officers in the development of risk-based performance standards aimed at reducing the risk of exposure to consumers. Full article

One Health (OH) is an integrative approach to human, animal, and environmental health and can be used as a comprehensive indicator for comparative purposes. Although an OH index has been proposed for comparing cities, states, and countries, to date, no practical study has compared countries using this approach. Accordingly, this study aimed to assess OH initiatives using a survey with a veterinary public health focus. The questionnaire contained 104 quantitative questions and was sent to representatives of governmental institutions of 32 countries in the Americas. After exclusion criteria were considered, a total of 35 questionnaires from 17 countries were analyzed, with country names remaining undisclosed during the statistical analyses to protect potentially sensitive information. Principal component analysis (PCA) of health parameters in Latin America and the Caribbean (LAC) as a function of country perception (self-vector) showed that food safety was ranked higher than public policies (p = 0.009), and that both (p = 0.003) were ranked higher than institutional routines related to zoonosis programs. National policies in accordance with international standards, regulations, recommendations, and guidelines was considered the standout topic for public policy, with higher-ranking topics including standard. Meanwhile, challenging topics included tools, preparedness, governance, and research. Food safety showed both strengths and challenges in the coordination of its activities with other sectors. Food safety communication was scored as a strength, while foodborne diseases prevention was ranked as a challenge. Institutional routines for zoonosis maintained both strong and challenging topics in the execution and implementation of attributions and daily routine. Thus, the survey showed that topics such as access to and compliance with international guidelines and intercountry integration were ranked higher than in-country articulation, particularly among food safety, zoonoses, and environmental institutions. Full article

A third of the world’s ecosystems are considered degraded, and there is an urgent need for protection and restoration to make the planet healthier. The Sustainable Development Goals (SDGs) target 15.3 aims at protecting and restoring the terrestrial ecosystem to achieve a land degradation-neutral world by 2030. Land restoration through inclusive and productive growth is indispensable to promote sustainable development by fostering climate change-resistant, poverty-alleviating, and environmentally protective economic growth. The SDG Indicator 15.3.1 is used to measure progress towards a land degradation-neutral world. Earth observation datasets are the primary data sources for deriving the three sub-indicators of indicator 15.3.1. It requires selecting, querying, and processing a substantial historical archive of data. To reduce the complexities, make the calculation user-friendly, and adapt it to in-country applications, a module on the FAO’s SEPAL platform has been developed in compliance with the UNCCD Good Practice Guidance (GPG v2) to derive the necessary statistics and maps for monitoring and reporting land degradation. The module uses satellite data from Landsat, Sentinel 2, and MODIS sensors for primary productivity assessment, along with other datasets enabling high-resolution to large-scale assessment of land degradation. The use of an in-country land cover transition matrix along with in-country land cover data enables a more accurate assessment of land cover changes over time. Four different case studies from Bangladesh, Nigeria, Uruguay, and Angola are presented to highlight the prospect and challenges of monitoring land degradation using various datasets, including LCML-based national land cover legend and land cover data. Full article

Rural and semi-urban areas in arid/semi-arid regions are facing severe water scarcity and a series of environmental challenges nowadays, specifically due to rapid urbanization and economic development, climate change, population growth, increasing water demand, influxes of refugees caused by war and regional political conflict, etc. To solve the emerging problems, the safe reuse of treated wastewater in agriculture can provide an additional water resource for countries with high water scarcity. The aim of this study was to investigate the treatment performance and effectiveness of small decentralized wastewater treatment (DWWT) technologies treating high-strength wastewater with concentrations far beyond the European Union testing ranges of parameters such as five-day biochemical oxygen demand (BOD₅ > 500 mg/L), chemical oxygen demand (COD > 1000 mg/L), or total suspended solids (TSS > 700 mg/L). Four (4) commercially available DWWT technologies with a design capacity of 4–8 PE (population equivalent) were selected and operated with various wastewater compositions in Leipzig, Germany. The technologies were (i) the moving bed biofilm reactor (MBBR), (ii) the sequencing batch reactor (SBR), (iii) the membrane bioreactor (MBR) and (iv) the aerated vertical-flow constructed wetland (AVFCW). This study results clearly demonstrated that the EU-certified small DWWT technologies are quite capable of treating high-strength wastewater and can provide high-quality treated water for safe reuse in rural communities of arid and semi-arid regions. During operation with high-strength wastewater with a mean inflow BOD₅, COD and TSS concentrations of 1532 ± 478, 2547 ± 830 and 546 ± 176 mg/L, a low mean BOD₅ (<10 mg/L), COD (<70 mg/L) and TSS (<15 mg/L) in the outflow of the four systems showed removal efficiency of BOD₅ (>99%), COD (>97%) and TSS (>97%), and met the maximum allowable limit value of water quality class A for reuse in agriculture according to Jordanian and Omani standard. The MBR showed almost a complete removal of Escherichia coli (E. coli) in a range of 6.1–6.9-log removal in the outflow during all three experimental phases and performed best for BOD₅, COD, TSS and pathogen removal when treating high-strength wastewater if properly maintained to prevent potential fouling and clogging of the membrane. Before the final permitting process, long-term monitoring under local temperature and climatic conditions as well as guidelines based on local needs (e.g., in Jordan, Oman, etc.) should be developed to guarantee a minimum level of performance standards of such small DWWT technologies and requirements for operation and maintenance (O&M). Full article

(1) Background: At present, the only potency assay approved in China for the in-country testing of botulinum toxin type A for injection products is the mouse bioassay (MBA). The Chinese market for neurotoxin products is rapidly expanding, but MBAs are subject to high variability due to individual variations in mice, as well as variations in injection sites, in addition to the limited number of batches tested for one MBA. Compared with the mLD₅₀ method, the cell-based potency assay (CBPA) developed for the potency testing of onabotulinumtoxinA (BOTOX) by AbbVie not only does not use any experimental animals but also allows for significant time and cost savings. Due to the significant benefits conferred by the replacement of the mLD₅₀ assay with CBPA in China, the CBPA method has been transferred, validated, and cross-validated to demonstrate the equivalence of the two potency methods. (2) Methods: The differentiated SiMa cells were treated with both BOTOX samples and the reference standard, and the cleaved SNAP25₁₉₇ in the cell lysates was quantified using Chemi-ECL ELISA. A 4-PL model was used for the data fit and sample relative potency calculation. The method accuracy, linearity, repeatability, and intermediate precision were determined within the range of 50% to 200% of the labeled claim. A statistical equivalence of the two potency methods (CBPA and mLD₅₀) was initially demonstrated by comparing the AbbVie CBPA data with NIFDC mLD₅₀ data on a total of 167 commercial BOTOX lots (85 50U lots and 82 100U lots). In addition, six lots of onabotulinumtoxinA (three 50U and three 100U) were re-tested as cross-validation by these two methods for equivalence. (3) Results: The overall assay’s accuracy and intermediate precision were determined as 104% and 9.2%, and the slope, R-square, and Y-intercept for linearity were determined as 1.071, 0.998, and 0.036, respectively. The repeatability was determined as 6.9%. The range with the acceptable criteria of accuracy, linearity, and precision was demonstrated as 50% to 200% of the labeled claim. The 95% equivalence statistic test using margins [80%, 125%] indicates that CBPA and mLD₅₀ methods are equivalent for both BOTOX strengths (i.e., 50U and 100U). The relative potency data from cross-validation were within the range of ≥80% to ≤120%. (4) Conclusions: The CBPA meets all acceptance criteria and is equivalent to mLD₅₀. The replacement of mLD₅₀ with CBPA is well justified in terms of ensuring safety and efficacy, as well as for animal benefits. Full article

Sierra Leone is an electricity-poor country with one of the lowest electricity consumption per capita rates across sub-Saharan Africa. Yet, with ambitious targets to transform and stimulate its economy in the coming decades, energy demand forecasting becomes an integral component of successful energy planning. Through applying the MAED-D (version 2.0.0) demand software, this research study aims to generate Sierra Leone’s electricity demand forecasts from 2023 to 2050. Three novel scenarios (baseline-, high-, and low-demand) are developed based on socio-economic and technical parameters. The baseline scenario considers the current electricity sector as business-as-usual; the high-demand scenario examines an ambitious development future with increased economic diversification and mechanisation, and the low-demand scenario examines more reserved future development. The modelled scenario results project an increase in electricity demand ranging from 7.32 PJ and 12.23 PJ to 5.53 PJ for the baseline-, high-, and low-demand scenarios, respectively, by 2050. This paper provides a base set of best-available data needed to produce an electricity demand model for Sierra Leone which can be used as a capacity-building tool for in-country energy planning alongside further integration into data modelling pipelines. Full article

The increasing global population and in-country migration have a significant impact on global land use land cover (LULC) change, which reduces green spaces and increases built-up areas altering the near-surface radiation and energy budgets, as well as the hydrological cycle over an urban area. The LULC change can lead to a combination of hazards such as increasing urban temperatures and intensified rainfall, ultimately resulting in increased flooding. This present study aims to discuss the changing pattern in urban temperature, daily rainfall, and flooding in Jakarta. The daily urban temperature and daily rainfall were based on a 30-year dataset from three meteorological stations of Jakarta in the period between 1987 and 2013. The changing trend was analyzed by using the Mann–Kendall and the Pettitt’s tests. The relation between daily rainfall and flooding was analyzed using a 30-year flooding dataset collected from several sources including the international disaster database, research, and newspaper. The results show that there was an increasing trend in the daily temperature and the daily rainfall in Jakarta. The annual maximum daily temperature showed that an increasing trend started in 2001 at the KMY station, and in 1996 at the SHIA station. In general, the highest annual maximum daily temperature was about 37 °C, while the lowest was about 33 °C. Moreover, the maximum daily rainfall started increasing from 2001. An increase in the maximum daily rainfall was observed mainly in January and February, which coincided with the flood events recorded in these months in Jakarta. This indicates that Jakarta is not only vulnerable to high urban temperature but also to flooding. While these two hazards occur in distinct timeframes, there is potential for their convergence in the same geographical area. This study provides new and essential insights to enhance urban resilience and climate adaptation, advocating a holistic approach required to tackle these combined hazards. Full article

This paper explores the extent to which International Financial Reporting Standards (IFRS)-wide support (regardless of the institutional context) is warranted. Drawing from an institutional theory framework, it investigates the extent to which practitioners in a code-law-rooted country perceive the materialization of claimed benefits of IFRS and the institutional forces that may hinder the IFRS’s institutionalization process. An interpretative approach based on qualitative research was adopted. Longitudinal evidence was gathered from 34 in-depth semi-structured in-person interviews with Portuguese preparers conducted in 2009 (just before an IFRS-based accounting system was adopted) and 2017 (at a post-implementation mature stage). Qualitative data analysis was carried out using a thematic coding scheme derived from the adopted theoretical framework: legitimacy and economic (operational and financial) benefits and institutional contradictions (IFRS’s in-country adequacy). The main results highlight that, while legitimacy benefits have been broadly recognized, economic benefits were scantly perceived due to the enduring code-law institutional logic embedded in the Portuguese context. Consistently, a high possibility of decoupling and manipulating accounts was acknowledged, which in turn casts reservations not only about the material consequences of the international convergence process to IFRS but also about its contribution towards sustainable development. Given that a major academic debate persists on whether accounting harmonization is beneficial, this research’s findings fill this gap and provide valuable insights for future research, practice, and regulation. Particularly, our findings enhance the urgency of developing institutional adjustments, such as changing national culture, on behalf of the Anglo-Saxon-based institutional approach of IFRS. Full article

To undertake the modernization of Azerbaijan’s water sector, the government of Azerbaijan collaborated with the Food and Agriculture Organization (FAO) of the United Nations (UN) in a comprehensive initiative to explore stakeholder perceptions on in-country water governance. The research approach, designed by national water experts and the authors, resulted in the development and administration of a stakeholder engagement and perception survey. The survey, implemented in 2022, elicited responses from a total of 219 state and non-state actors. To enrich interpretations of the survey, this study furthers the analysis of the government commissioned FAO stakeholder survey results and sheds additional insights as to where stakeholders see problems in water governance processes. This independent study informs the broader FAO project and generates supplemental recommendations to the Azerbaijani government for legislative and executive-level action to make Azerbaijan’s water sector more resilient as the climate changes and water insecurity increases. Even though an impressive number of 219 stakeholders participated in the survey, 80% of the responses were from state stakeholders, thus introducing significant bias into the dataset. In order to cope with the bias and make the best of the dataset, the authors analyzed the responses with a customized categorical methodology. Stakeholders were categorized into state, non-state, decision-maker, or executive groups and were examined for trends using various Pareto analyses. Interpretation of the survey responses reveals that, while stakeholders in the water sector interact through informal and formal means, stakeholder groups, to a large extent, lack an understanding of the barriers to stakeholder engagement in water-related policy matters. The stakeholders that indicate understanding of challenges accompanying water policy engagement note a lack of data, a lack of human and institutional capacity, and a lack of financial support to be some of the most common obstacles encountered in the sector. Furthermore, perceptions differ regarding the need for governance reform, the criticality of climate change, institutional resistance to change, policy or practice gaps, transparency, and variables needed for successful stakeholder engagement across all sectors. Such variations in perceptions illustrate the need for restructuring stakeholder interactive platforms and financial channeling to lead to better water governance and water management. Full article

We conducted a systematic review of healthcare-associated outbreaks and cross-sectional surveys related to the contamination of antiseptics, disinfectants, and hand hygiene products in healthcare settings in low- and middle-income countries (PROSPERO CRD42021266271). Risk of bias was assessed by selected items of the ORION and MICRO checklists. From 1977 onwards, 13 outbreaks and 25 cross-sectional surveys were found: 20 from Asia and 13 from Africa. Products most associated with outbreaks were water-based chlorhexidine, chlorhexidine-quaternary ammonium compound combinations (7/13), and liquid soap products (4/13). Enterobacterales (including multidrug-resistant Enterobacter cloacae, Klebsiella pneumoniae, and Serratia marcescens) and non-fermentative Gram-negative rods were found in 5 and 7 outbreaks and in 34.1% and 42.6% of 164 isolates, respectively, from cross-sectional surveys. Risk factors included preparation (place, utensils, or tap water high and incorrect dilutions), containers (reused, recycled, or inadequate reprocessing), and practices (topping-up or too long use). Potential biases were microbiological methods (neutralizers) and incomplete description of products’ identity, selection, and denominators. External validity was compromised by low representativeness for remote rural settings and low-income countries in sub-Saharan Africa. Outstanding issues were water quality, biofilm control, field-adapted containers and reprocessing, in-country production, healthcare providers’ practices, and the role of bar soap. A list of “best practices” to mitigate product contamination was compiled. Full article

Biochar and nitrogen fertilizers are known to increase soil carbon storage and reduce soil nitrogen loss as amendments, suggesting a promising strategy for highly effectively increasing soil productivity. However, few studies have explored the mechanisms of their effects on crop yield in terms of active carbon fraction and enzyme activity, which ultimately limits the potential for the application of biochar in combination with nitrogen fertilizers. To evaluate the effect of biochar and nitrogen fertilizer on the improvement of black soils in northeast China, a field experiment was conducted in the black soil to compare and analyze the application methods on total organic carbon (TOC), total nitrogen (TN), enzyme activities, and maize yields. Biochar rates: CK, C1, C2, and C3 (0, 9.8, 19.6, and 29.4 Mg·ha⁻¹); N fertilizer rates: N1/2 and N (30 and 60 kg·ha⁻¹). Results indicated that biochar and N fertilizer amendments significantly ameliorated soil fertility, such as TOC and TN, compared to the unamended soil. The TOC levels in the C3 treatment increased by 35.18% and the TN levels by 23.95%. The improvement in TN is more significant when biochar is blended with N fertilizer. Biochar blended with N fertilizer increased maize cellulase, urease, and invertase activities by an average of 53.12%, 58.13%, and 16.54%, respectively. Redundancy analysis showed that TOC, TN, and MBN contributed 42%, 16.2%, and 22.2%, respectively, to the maize yield indicator. Principal component analysis showed that reduced N fertilizer was more effective in improving yields, with a maximum yield increase of 50.74%. Biochar blended with N fertilizer is an effective strategy to improve the fertility and productivity of black soils in northeast China, while nitrogen fertilizer reduction is feasible and necessary for maintaining grain yield. Full article

Globally to date, established international standards for animal welfare, a priority of sustainable agriculture, have primarily focused on large-scale producers. However, across Latin America, including in Ecuador’s Galápagos Islands, smallholder farms play a critical role in food safety and security. We assessed five basic animal welfare measures (feed and water access, shelter availability and housing systems, animal health management, animal behavior, and timely euthanasia) for poultry, pigs, and cattle on Floreana Island, Galápagos. Utilizing assessment standards from multiple US sources and international standards, we developed a questionnaire and used it to conduct in-depth interviews during 4–5 July 2022 with eight participating producers, representing 75% of animal agriculture on Floreana. While we identified opportunities to enhance competencies in animal health management and timely euthanasia, farms performed well in the other assessed measures. Future work should promote knowledge transfer and in-country capacity building in farm biosecurity, access to veterinary care, antimicrobial resistance surveillance, and euthanasia methods. Efforts to positively impact smallholder farm livelihoods in Galápagos—one of the most biodiverse and protected ecosystems on the planet—will sustainably support human health through the interconnected realms of animal health and welfare, wildlife and environmental health, and food safety and security. Full article

The Xpert^® Xpress SARS-CoV-2 and Xpert^® Xpress SARS-CoV-2/Flu/RSV tests were rapidly developed and widely used during the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic. In response to emerging genetic variability, a new SARS-CoV-2 target (RNA-dependent RNA-polymerase) has been added to both tests: Xpert^® Xpress CoV-2 plus and Xpert^® Xpress CoV-2/Flu/RSV plus test. A rapid evaluation of both tests was performed in South Africa, using residual respiratory specimens. Residual respiratory specimens (n = 125) were used to evaluate the Xpert^® Xpress CoV-2 plus test and included 50 genotyped specimens. The Xpert^® Xpress CoV-2/Flu/RSV plus test was assessed using 45 genotyped SARS-CoV-2 specimens, 10 influenza A, 10 influenza B and 20 respiratory syncytial virus specimens. Results were compared to in-country standard-of-care tests. Genotyped specimens tested the performance of the test under pressure from circulating SARS-CoV-2 variants of concern. Reference material was included to assess the test limits and linearity. The Xpert^® Xpress CoV-2 plus test performance compared to reference results across residual respiratory specimens was good (positive percentage agreement (PPA) = 95.2%, negative percentage agreement (NPA) = 95.0%) The Xpert^® Xpress CoV-2/Flu/RSV plus test showed good performance across all residual respiratory specimens (PPA = 100%, NPA = 98.3%). All genotyped variants of concern were detected by both tests. The Xpert^® Xpress CoV-2 plus and Xpert^® Xpress CoV-2/Flu/RSV plus tests can be used to diagnose SARS-CoV-2, and to diagnose and differentiate SARS-CoV-2, influenza A, influenza B and respiratory syncytial virus, respectively. The NPA was lower than the recommended 99%, but was influenced by the low number of negative specimens tested. The variants of concern assessed did not affect test performance. It is recommended that sites perform their own assessments compared to in-country standard-of-care tests. Full article

This paper focuses on the use of mobile technology to assist teaching and learning in distance education. It aims to investigate teaching behaviour in mobile pedagogy and examine the impact of technology on current education. A case study was conducted through semi-structured interviews with a cohort of 30 Chinese lecturers who taught English through online tutoring. Thematic analysis was used to analyse the interview data and the assessment was based on teacher perceptions of mobile pedagogy. The impact of technology on the current educational environment is discussed through an analysis of mobile pedagogy and teacher perceptions. The findings show that mobile pedagogy is highly regional in practice and nature and features in-country software applications and social communication tools. Despite the attributes of connectivity and flexibility, mobile pedagogy only disrupted traditional teaching methods, leading to minimal changes to the education system. This study provides recommendations for the sustainable development of mobile pedagogy for future education systems in the digital age. Full article

The Kingdom of Saudi Arabia (KSA) has an ambitious plan to install 40 GW of solar photovoltaic (PV) capacity via large scale projects (majority of which are >100 MW) across the country by 2030. These projects are required to achieve a threshold percentage of the overall project cost as in country expenditure, termed “local content”. This threshold will rise to 40–45% by 2028, ensuring solar projects do not simply become investment opportunities for overseas companies but deliver jobs and skills development within the Kingdom. Local content is assessed across all aspects of a PV system—module, inverter, structure, etc. Typically, the PV module cost can range between ~34% to ~44% of the overall system cost. However, 56% of this module cost represents the manufacture of the solar cells. To maximise local content, the opportunity for KSA lies in module fabrication of imported solar cells. This study assesses the capacity readiness of the KSA to meet this opportunity in relation to its 2030 targets and the current policy landscape. The recently completed 300 MW Sakaka project had a local content of >30% based on the developers’ website and >35% based on our private communication. Our results indicate that this would rise by 19% for a project like Sakaka if all modules were fabricated in-country rather than being imported. The work also projected a shortfall in terms of in-country module fabrication capacity with clear implications to local content targets. The results show a shortfall in the range of 35% to 75% by 2030 depending on the adopted KSA energy policies and government support. KSA is seen to have good capacity in other PV components such as steel and float glass. In-country distribution of imported solar cells to module fabrication centres and subsequent distribution to PV farm sites has also been assessed. Our analyses indicate that a two-location solution reduces cost with new module fabrication capacity sited near Jeddah and Riyadh, minimising storage times, delivery time to site and distribution waiting times. Full article