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Search Results (987)

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14 pages, 683 KiB  
Article
Fortification of Orange and Apple Juices with Ferulic Acid: Implications for Food Safety and Quality
by Maribel Abadias, Gloria Bobo, Marina Anguera, Jordi Ortiz-Solà and Ingrid Aguiló-Aguayo
Foods 2024, 13(20), 3288; https://doi.org/10.3390/foods13203288 (registering DOI) - 16 Oct 2024
Abstract
In recent years, the awareness of healthier lifestyles among consumers has driven to an increased interest in more natural, nutritious, and low-processed foods. Ferulic acid, one of the most abundant phenolic acids in plants, has demonstrated a wide spectrum of antimicrobial activities and [...] Read more.
In recent years, the awareness of healthier lifestyles among consumers has driven to an increased interest in more natural, nutritious, and low-processed foods. Ferulic acid, one of the most abundant phenolic acids in plants, has demonstrated a wide spectrum of antimicrobial activities and a range of biomedical effects, including antioxidant, antiallergic, hepatoprotective, anticarcinogenic, anti-inflammatory, and antithrombotic, among others. The objective of this work was to study the antilisterial effect of ferulic acid (FA, 1500 mg/L) on fresh, ready-to-eat orange (FOJ) and apple (FAJ) juices and evaluate its effect on product quality. The results showed that FA reduced the Listeria monocytogenes population after 9 days of storage at 4 °C, but no effect on the indigenous microbiota was observed. The titratable acidity and color significantly changed. The antioxidant capacity and total phenolic content significantly increased with the addition of FA, being at least two times greater in fortified juices. FAJ and FOJ containing FA were scored lower (6.8 and 5.7 on a 9-point hedonic scale, respectively) than their respective controls. Overall, our results demonstrated that FA treatment could be a useful strategy to maintain the safety of fresh apple and orange juices and increase the antioxidant activity and phenolic content. The potential industrial applications and health benefits of the fortification of fruit juices with FA should be further explored. Full article
(This article belongs to the Section Food Quality and Safety)
18 pages, 1427 KiB  
Article
Ground Ivy (Glechoma hederacea L.) as an Innovative Additive for Enriching Wheat Bread: Study on Flour Fermentation Properties, Dough Rheological Properties and Bread Quality
by Karolina Pycia, Agata Maria Pawłowska, Zuzanna Posadzka and Joanna Kaszuba
Appl. Sci. 2024, 14(20), 9392; https://doi.org/10.3390/app14209392 (registering DOI) - 15 Oct 2024
Viewed by 242
Abstract
The aim of the study was to assess the effect of water infusion of dried and crushed ground ivy (GH) on the fermentation properties of wheat flour (WF), farinographic properties of flour and dough (WD) and the quality of the obtained bread. In [...] Read more.
The aim of the study was to assess the effect of water infusion of dried and crushed ground ivy (GH) on the fermentation properties of wheat flour (WF), farinographic properties of flour and dough (WD) and the quality of the obtained bread. In the tested systems, tap water was replaced with water infusion with GH (m/v) at a concentration of 1% (A), 3% (B) and 5% (C). As part of the research methodology, the fermentation properties of flour and rheological properties of dough were assessed using a farinograph, and bread was obtained using a single-phase method using yeast and its quality was assessed. As part of this, the antioxidant potential and the profile and level of polyphenol content were determined. It was shown that replacing water with GH infusions shortened the total fermentation time of the dough and reduced the fermentation capacity of the dough. In the farinographic evaluation, an increase in flour water absorption (54.0–57.0%), dough development time (2.3–7.6 min), dough stability and softening were observed with an increase in the concentration of the added GH infusion. In turn, the volume of the loaf and the specific volume of the bread decreased with increasing the concentration of the GH infusion. The bread crumb darkened, and the elasticity and chewiness of the crumb decreased in relation to the control sample. In turn, the presence of GH infusion did not significantly affect the hardness of the crumb. As the concentration of the added GH infusion increased, an increase in the antioxidant potential of bread and the content of polyphenols and flavonoids was observed, and the UPLC-PDA-MS/MS analysis allowed the identification of 11 polyphenols in the bread. Full article
(This article belongs to the Section Food Science and Technology)
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<p>Fermentographic graphs of tested doughs: <b>control</b>—control sample with water; <b>A</b>—dough with 1% infusion; <b>B</b>—dough with 3% infusion; <b>C</b>—dough with 5% infusion.</p>
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<p>Appearance of loaves and their cross-sections of tested breads with <span class="html-italic">G. hederacea</span> L. infusions: <b>control</b>—bread made of dough with water; <b>A</b>—bread made of dough with 1% infusion; <b>B</b>—bread made of dough with 3% infusion; <b>C</b>—bread made of dough with 5% infusion.</p>
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14 pages, 4354 KiB  
Article
Effect of Sinapine on Microstructure and Anti-Digestion Properties of Dual-Protein-Based Hydrogels
by Youdong Li, Mengxin Duan, Guoyan Liu, Li Liang, Xiaofang Liu, Jixian Zhang, Chaoting Wen and Xin Xu
Foods 2024, 13(20), 3237; https://doi.org/10.3390/foods13203237 - 11 Oct 2024
Viewed by 412
Abstract
Sinapine is a natural polyphenol from the cruciferous plant family that has anti-aging effects but is low in bioavailability. To improve the bioavailability and therapeutic effect of sinapine, sinapine-crosslinked dual-protein-based hydrogels were prepared using soy protein isolate as a cross-linking agent. The preparation [...] Read more.
Sinapine is a natural polyphenol from the cruciferous plant family that has anti-aging effects but is low in bioavailability. To improve the bioavailability and therapeutic effect of sinapine, sinapine-crosslinked dual-protein-based hydrogels were prepared using soy protein isolate as a cross-linking agent. The preparation conditions were optimized by single-factor experiments, and the optimal ratios were obtained as follows: the concentration of sinapine was 300 μg/mL; the water–oil ratio was 1:3. The encapsulation rate was greater than 95%, and the drug loading capacity was 3.5 mg/g. In vitro, digestion experiments showed that the dual-protein-based hydrogels as a drug carrier stabilized the release of sinapine and improved the bioavailability of sinapine by 19.3%. The IC50 of DPPH antioxidants was 25 μg/mL as determined by in vitro digestion, and the antioxidant capacity of ABTS was about 20% higher than that of glutaraldehyde control. This is due to the addition of sinapine to enhance the antioxidant properties of the system. It can be seen that the developed hydrogels have potential applications in related fields, such as food nutrition fortification and drug delivery. Full article
(This article belongs to the Section Food Physics and (Bio)Chemistry)
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<p>Effects of different water–oil ratios, sinapine concentrations, and protein concentrations on particle size potential, drug loading, and entrapment rate of gel (<b>a</b>,<b>b</b>) W/O; (<b>c</b>,<b>d</b>) sinapine concentration; (<b>e</b>,<b>f</b>) protein concentration. Different letters above each column represent significant differences (<span class="html-italic">p</span> &lt; 0.05).</p>
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<p>Optical micrograph of emulsion without the protein crosslinker (<b>a</b>,<b>b</b>) and different emulsification methods: (<b>c</b>,<b>e</b>) stir; (<b>d</b>,<b>f</b>) vortex.</p>
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<p>SEM photo of sinapine-loaded double protein composite gel. (<b>a</b>–<b>c</b>) No sinapine was added and the protein concentration was 1.5%; (<b>d</b>–<b>f</b>) sinapine was added and the protein concentration was 1.5%; (<b>g</b>–<b>i</b>) added sinapine and protein concentration was 0.5%.</p>
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<p>TEM photo of sinapine-loaded double protein composite gel.</p>
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<p>Swelling degree of sinapine loaded double protein composite gel (water–oil ratio: 1:2, 1:3, 1:4, 1:5, 1:6; protein concentration: 0.5%, 0.1%, 0.05%, 0.01%, 0.005%; the concentration of sinapine was 100, 200, 300, 400, 500 μg/mL). Different letters above each column represent significant differences (<span class="html-italic">p</span> &lt; 0.05).</p>
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<p>FT-IR of sinapine-loaded double protein composite gel.</p>
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<p>In vitro digestibility and antioxidant properties analysis of dual-protein-based hydrogels. (<b>a</b>) In vitro digestibility of free sinapine and sinapine-crosslinked dual-protein-based hydrogels; (<b>b</b>) antioxidant capacity of ABTS after in vitro digestion; (<b>c</b>) antioxidant capacity of DPPH after in vitro digestion. Different letters above each column represent significant differences (<span class="html-italic">p</span> &lt; 0.05).</p>
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17 pages, 19977 KiB  
Article
Feasibility of Using Ferronickel Slag as a Sustainable Alternative Aggregate in Hot Mix Asphalt
by Lisley Madeira Coelho, Antônio Carlos Rodrigues Guimarães, Claudio Rafael Cicuto Landim Alves Moreira, Graziella Pereira Pires dos Santos, Sergio Neves Monteiro and Pedro Henrique Poubel Mendonça da Silveira
Sustainability 2024, 16(19), 8642; https://doi.org/10.3390/su16198642 - 6 Oct 2024
Viewed by 870
Abstract
Ferronickel slag (FNS) is a byproduct produced during ferronickel alloy manufacturing, primarily used in the manufacturing of stainless steel and iron alloys. This material is produced by cooling molten slag with water or air, posing significant disposal challenges, as improper storage in industrial [...] Read more.
Ferronickel slag (FNS) is a byproduct produced during ferronickel alloy manufacturing, primarily used in the manufacturing of stainless steel and iron alloys. This material is produced by cooling molten slag with water or air, posing significant disposal challenges, as improper storage in industrial yards can lead to environmental contamination. This study investigates the chemical and mineralogical characteristics of reduction ferronickel slag (RFNS) and its potential use as an alternative aggregate in hot mix asphalt (HMA). The research is based on the practical application of HMA containing RFNS in an experimental area, specifically the parking lot used by buses transporting employees of Anglo American, located at the Codemin Industrial Unit in Niquelândia, Goiás, Central Brazil. Chemical analysis revealed that RFNS primarily consists of MgO, Fe2O3, and SiO2, which are elements with minimal environmental impact. The lack of significant calcium content minimizes concerns about expansion issues commonly associated with calcium-rich slags. The X-ray diffractogram indicates a predominantly crystalline structure with minerals like Laihunite and Magnetite, which enhances wear and abrasion resistance. HMA containing 40% RFNS was tested using the Marshall methodology, and a small experimental area was subsequently constructed. The HMA containing RFNS met regulatory specifications and technological controls, achieving an average resilient modulus value of 6323 MPa. Visual inspections conducted four years later confirmed that the pavement remained in excellent condition, validating RFNS as a durable and effective alternative aggregate for asphalt mixtures. The successful application of RFNS not only demonstrates its potential for local road paving near industrial areas but also underscores the importance of sustainable waste management solutions. This research highlights the value of academia–industry collaboration in advancing environmentally responsible practices and reinforces the contribution of RFNS to enhancing local infrastructure and promoting a more sustainable future. Full article
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<p>A flowchart of the experimental procedure for this study.</p>
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<p>A sample of ferronickel slag used in this study.</p>
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<p>Limestone aggregates in the following fractions: coarse aggregate (B-1), medium aggregate (B-0), and dust (pó).</p>
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<p>The location of the experimental area.</p>
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<p>The initial condition of the experimental area.</p>
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<p>Scanning electron microscopy (SEM) images of reject powders at different magnifications: (<b>a</b>) 40×, showing the overall particle distribution; and (<b>b</b>) 150×, highlighting the detailed morphology of the particles.</p>
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<p>EDX map of ferronickel slag.</p>
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<p>The X-ray diffractogram obtained for the RFNS sample.</p>
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<p>OM image of sample with 40× magnification.</p>
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<p>The particle size curve of the HMA containing RFNS and the normative design limits.</p>
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<p>Parking lot for boarding and disembarking of passengers concluded.</p>
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<p>Status of experimental area. (<b>a</b>) Pavement disaggregation. (<b>b</b>) Point disaggregation.</p>
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<p>Status of experimental area after four years of implementation. (<b>a</b>) Frontal view. (<b>b</b>) Side view.</p>
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26 pages, 19393 KiB  
Article
ML Approaches for the Study of Significant Heritage Contexts: An Application on Coastal Landscapes in Sardinia
by Marco Cappellazzo, Giacomo Patrucco and Antonia Spanò
Heritage 2024, 7(10), 5521-5546; https://doi.org/10.3390/heritage7100261 - 5 Oct 2024
Viewed by 567
Abstract
Remote Sensing (RS) and Geographic Information Science (GIS) techniques are powerful tools for spatial data collection, analysis, management, and digitization within cultural heritage frameworks. Despite their capabilities, challenges remain in automating data semantic classification for conservation purposes. To address this, leveraging airborne Light [...] Read more.
Remote Sensing (RS) and Geographic Information Science (GIS) techniques are powerful tools for spatial data collection, analysis, management, and digitization within cultural heritage frameworks. Despite their capabilities, challenges remain in automating data semantic classification for conservation purposes. To address this, leveraging airborne Light Detection And Ranging (LiDAR) point clouds, complex spatial analyses, and automated data structuring is crucial for supporting heritage preservation and knowledge processes. In this context, the present contribution investigates the latest Artificial Intelligence (AI) technologies for automating existing LiDAR data structuring, focusing on the case study of Sardinia coastlines. Moreover, the study preliminary addresses automation challenges in the perspective of historical defensive landscapes mapping. Since historical defensive architectures and landscapes are characterized by several challenging complexities—including their association with dark periods in recent history and chronological stratification—their digitization and preservation are highly multidisciplinary issues. This research aims to improve data structuring automation in these large heritage contexts with a multiscale approach by applying Machine Learning (ML) techniques to low-scale 3D Airborne Laser Scanning (ALS) point clouds. The study thus develops a predictive Deep Learning Model (DLM) for the semantic segmentation of sparse point clouds (<10 pts/m2), adaptable to large landscape heritage contexts and heterogeneous data scales. Additionally, a preliminary investigation into object-detection methods has been conducted to map specific fortification artifacts efficiently. Full article
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<p>Defensive heritage artifacts. Capo Boi tower, Sinnai—Cagliari (<b>a</b>). Sant’Ignazio fortress, Calamosca—Cagliari (<b>b</b>). Position no. 5 of Stronghold V, Porto Ferro—Alghero (<b>c</b>). Position no. 2 (hypothesis) of Stronghold XI, Alghero (<b>d</b>).</p>
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<p>Map of the location of the presented case studies related to the research framework of the present contribution, Sardinia (Italy).</p>
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<p>Case study 1. The case study 1 area is located in the southern region of Sardinia and covers the whole extension of Cagliari town and hinterland.</p>
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<p>Case study 2. The case study 2 area is located in the northwest region of Sardinia and covers the whole extension of Alghero town and hinterland.</p>
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<p>Map of the data provided by the Sardinia Region. The stripes available from the two airborne LiDAR surveys are located on this map. The two surveys were carried out using two different sensors, as detailed in <a href="#heritage-07-00261-t001" class="html-table">Table 1</a>.</p>
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<p>Methodological schema. The workflow is developed for heterogeneous landscape heritage frameworks leveraging multiple existing airborne LiDAR datasets (1). The case studies that have been selected thus not only pertain to distinguished heritage contexts but are characterized by different acquisition scales and densities. The second stage of the methodology consists of applying unsupervised (2a) and data fusion strategies (2b) to prepare reference data for DL classification model training (2c). Finally, a preliminary investigation of object detection strategies (3) addresses the system mapping and artifact recognition challenges of historical defensive heritage using point cloud deep learning approaches [<a href="#B56-heritage-07-00261" class="html-bibr">56</a>].</p>
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<p>Sentinel-2 data fusion approaches. Vector water mask generation for areal segmentation (<b>a</b>). Band 8 NIR projection on DSM mesh for scalar value interpolation (<b>b</b>).</p>
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<p>Map of the location of the training dataset (case study 1, Cagliari). The red tiles are about the training set, while the green blocks relate to the point cloud tiles used to validate the model.</p>
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<p>Map of the test dataset areas, A, B, C, and D locations. The test point clouds are in the case study 2 area (Alghero).</p>
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<p>Label generation workflow: from a point feature class to a 3D geometry.</p>
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<p>Point cloud echo information, reflectance intensity, and newly calculated scalar field comparison for geometrical and digital number filtering unsupervised segmentation. Number of returns (<b>a</b>). Intensity (<b>b</b>). Data fusion near infrared from Sentinel 2 band 8, 784 nm–899.5 nm (<b>c</b>). λ<sub>3</sub> eigenvalue (normals) calculated on 2.5 m radius (<b>d</b>).</p>
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<p>Predictive model training results. The model performance is evaluated using the validation data from the Training dataset of case study 1 (Cagliari).</p>
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<p>Predictive model testing results. The model performance is evaluated using the test dataset A, B, C, and D of case study 2 (Alghero).</p>
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<p>Bounding box generation processing for reference data generation. The aim is to apply 3D deep learning for defensive heritage mapping. In this case, the three areas are focused on bunker class objects.</p>
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<p>Model validation graph, showing training and validation logarithmic loss functions during epochs. While the training loss function decreases, validation loss is constantly flat.</p>
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30 pages, 14702 KiB  
Article
Environment as Palimpsest: Layers of Buddhist Imagery on Kyŏngju Namsan during the Unified Silla (668–935 CE) Period
by Elizabeth Lee
Religions 2024, 15(10), 1173; https://doi.org/10.3390/rel15101173 - 26 Sep 2024
Viewed by 419
Abstract
This study unpacks the Buddhist assimilation of Namsan (South Mountain) in Kyŏngju through the creation and aggregation of Buddhist sculptures and structures on its slopes during the seventh to tenth centuries. Though steeped in native lore regarding nature deities and efficacious rocks, auspicious [...] Read more.
This study unpacks the Buddhist assimilation of Namsan (South Mountain) in Kyŏngju through the creation and aggregation of Buddhist sculptures and structures on its slopes during the seventh to tenth centuries. Though steeped in native lore regarding nature deities and efficacious rocks, auspicious geological features such as Namsan were recast as part of a Buddhist landscape filled with manifestations of the Buddha and his attendants. These images served to demarcate claims of Buddhism’s place in the peninsula and were situated within sites that were previously marked and claimed by indigenous systems of belief. Employing an approach that draws parallels with David Harvey’s concept of urban environments as palimpsests, this paper reveals that Namsan was a multifaceted site, with military fortifications, temples, and rock-carved sculptures augmenting its spiritual and political significance. The repeated installation of Buddhist imagery ‘recovered’ the mountain, subsuming indigenous beliefs under Buddhist practices. This research finds that Namsan’s landscape was purposefully layered, reflecting the dialectical relationship between various communities and their religious and social practices over time. Analyzing Namsan as a palimpsest underscores the strategic appropriation of the mountain’s materiality and sacrality to establish a Buddhist territory deeply intertwined with the Silla elite’s politics and ideologies. Full article
(This article belongs to the Special Issue Buddhist Art, Artifact and Culture Worldwide)
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<p>Cultural Heritage sites on Namsan, Kyŏngju, North Kyŏngsang Province. Adapted from Kyŏngju munhwajae yŏn’guso (2000), <span class="html-italic">Kyŏngju Namsan</span>.</p>
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<p>Maitreya Buddha with attendant bodhisattvas (Samhwa Ridge Triad). From Namsan, North Kyŏngsang Province. ca. 644 CE, Silla (57 BCE–667 CE). Granite; Buddha h. 160 cm. Kyŏngju National Museum. Artwork in the public domain.</p>
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<p>Three Seated Buddhas. 835 CE, Unified Silla (668–935). Rock carving; h. 96 cm. Yunŭl Valley, Namsan, North Kyŏngsang Province. Photo taken by author (unless otherwise noted, all photos were taken by the author).</p>
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<p>Four Seated Buddhas and Buddha triad. First half of eighth century, Unified Silla (668–935). Rock carving; Central Buddha h. 4.25 m. Ch’ilburam, Namsan, North Kyŏngsang Province. Treasure no. 200.</p>
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<p>Detail of four-sided Buddha stone (from left: east side, west side, south side, north side). After Seo, “Kyŏngju Namsan Ch’ilburam”, p. 52.</p>
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<p>Sutra stone fragments. From the Ch’ilburam site, Ponghwa valley, Namsan, North Kyŏngsang Province. Eighth century, Unified Silla (668–935). Dark agalmatolite or granite. Kyŏngju National Museum. After Kyŏngju munhwajae yŏn’guso, <span class="html-italic">Kyŏngju namsan</span>, p. 312.</p>
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<p>Stone Seated Maitreya Bodhisattva. Second half of eighth century, Unified Silla (668–935). Rock carving; h. 1.4 m. Sinsŏn-am, Namsan, North Kyŏngsang Province. Treasure no. 199.</p>
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<p>Stone Seated Medicine Buddha from Samnŭng Valley, Namsan, North Kyŏngsang Province. Early ninth century, Unified Silla (668–935). Granite; h. 340 cm. National Museum of Korea. Artwork in the public domain.</p>
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<p>Stone Seated Buddha. Late eighth century, Unified Silla (668–935). Granite; h. 130 cm. Samnŭng Valley temple site 6, Namsan, North Kyŏngsang Province. Treasure no. 666.</p>
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<p>Standing Avalokitesvara. Second half of eighth century, Unified Silla (668–935). Rock carving; h. 152 cm. Samnŭng Valley, Namsan, North Kyŏngsang Province.</p>
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<p>Stone-incised Standing Buddha Triad and Seated Buddha Triad. Early ninth century, Unified Silla (668–935). Rock carving; Standing Buddha h. 317 cm; Seated Buddha h. 306 cm. Samnŭng Valley, Namsan, North Kyŏngsang Province.</p>
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<p>(Drawing of incised triads) after Kyŏngju kungnip kongwŏn.</p>
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<p>Medicine Buddha Triad. 801 CE, Unified Silla (668–935). Rock carving; h. 285 cm. Bangŏsan, Haman, South Kyŏngsang Province.</p>
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<p>Stone-incised Buddha. Ninth century, Unified Silla (668–935). Rock carving; h. 80 cm. Samnŭng Valley, Namsan, North Kyŏngsang Province.</p>
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<p>Seated Buddha of Sangsŏn Hermitage. Ninth century, Unified Silla (668–935). Rock carving; h. 5.21 m. Samnŭng Valley, Namsan, North Kyŏngsang Province.</p>
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<p>View from ridge of Samnŭng Valley, Namsan, North Kyŏngsang Province.</p>
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<p>Stone Seated Buddha. Late eighth—early ninth century, Unified Silla (668–935). Granite; h. 160 cm. Samnŭng Valley, Namsan, North Kyŏngsang Province.</p>
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17 pages, 1198 KiB  
Review
Supplementation with Folic Acid or 5-Methyltetrahydrofolate and Prevention of Neural Tube Defects: An Evidence-Based Narrative Review
by María de Lourdes Samaniego-Vaesken, Carmen Morais-Moreno, Alejandra Carretero-Krug, Ana María Puga, Ana María Montero-Bravo, Teresa Partearroyo and Varela-Moreiras Gregorio
Nutrients 2024, 16(18), 3154; https://doi.org/10.3390/nu16183154 - 18 Sep 2024
Viewed by 1572
Abstract
Background: Folic acid (FA), which in its chemical form is pteroylglutamic acid, is the fully oxidised, water-soluble, monoglutamic form of vitamin B9. This compound is part of the folate group but with higher bioavailability, and it is found in vitamin supplements and fortified [...] Read more.
Background: Folic acid (FA), which in its chemical form is pteroylglutamic acid, is the fully oxidised, water-soluble, monoglutamic form of vitamin B9. This compound is part of the folate group but with higher bioavailability, and it is found in vitamin supplements and fortified foods and drugs. Folate metabolism is complex and associated with various metabolic pathways, all of which confer protection on the cell and allow its survival. Methods: We conducted a non-systematic search of articles published in English and Spanish including controlled trials, cohort studies, systematic re-views, and meta-analyses were included, as well as key studies in animal models related to pharmacokinetic studies. Search terms encompassed: “folic acid”, “folates”, “5-metyltetrahydrofolate”, “5-MTHF”, “neural tube defects”, “supplementation”, “fortification”, AND “homocysteine” Results: A crucial role demonstrated for FA is to help prevent neural tube defects (NTDs). However, more studies are definitely still needed to establish 5-MTHF as a safe and effective therapeutic approach comparable with FA. Moreover, there is a lack of clinical studies that evaluate the efficacy of 5-MTHF supplementation in the prevention of NTDs. The present evidence-based narrative review discusses differences between FA and 5-MTHF in terms of structure, metabolism, bioavailability, clinical efficacy, and safety. Conclusions: Despite the potential value of 5-MTHF as an alternative to FA, clinical studies would be urgently needed to support the efficacy, dosage, timing, and/or safety of its use as a supplement. Full article
(This article belongs to the Section Nutrition in Women)
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<p>Methionine and folate cycles and connecting pathways. Created with BioRender.com.</p>
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<p>Folic acid and 5-MTHF chemical structures. Created with BioRender.com.</p>
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<p>Hierarchy of evidence for FA and 5-MTHF regarding neural tube defects (NTDs). A literature search was conducted in PubMed using the following search terms: ‘folic acid[title] AND neural tube defects[title] AND supplementation’; or ‘(5-MTHF[title] OR methyltetrahydrofolate[title]) AND neural tube defects[title] AND supplementation’. Created with BioRender.com.</p>
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18 pages, 2604 KiB  
Article
Cookies Fortified with Clitoria ternatea Butterfly Pea Flower Petals: Antioxidant Capacity, Nutritional Composition, and Sensory Profile
by Ribi Ramadanti Multisona, Kamila Myszka, Bartosz Kulczyński, Marcellus Arnold, Anna Brzozowska and Anna Gramza-Michałowska
Foods 2024, 13(18), 2924; https://doi.org/10.3390/foods13182924 - 15 Sep 2024
Viewed by 793
Abstract
This study aimed to fortify cookies to be functional food by adding Clitoria ternatea flower (CT) at concentrations ranging from 0 to 8%. Sensory profiling identified 6% CT as optimal for organoleptic attributes. The addition of CT did not significantly impact protein, lipid, [...] Read more.
This study aimed to fortify cookies to be functional food by adding Clitoria ternatea flower (CT) at concentrations ranging from 0 to 8%. Sensory profiling identified 6% CT as optimal for organoleptic attributes. The addition of CT did not significantly impact protein, lipid, and ash content but decreased energy value and increased insoluble and soluble fibre levels. The inclusion of 6% CT had a significant effect on the overall total phenolic content (TPC), which increased compared to the control sample. Antioxidative activity analyses showed enhanced antioxidative activity in ABTS, DPPH, ORACFL, and PCL assays. The addition of 6% CT inhibited hydroperoxide production in cookies. However, over a period of 6 weeks, a significant rise in peroxide value was observed during the 4th and 6th weeks of storing fortified cookies. All assessed products met the high microbiological quality standards. The sensory evaluation scores showed that CT can create cookies with health benefits and a good overall acceptance score. The texture of the cookies gradually became softer, but no significant changes in visual appearance were observed. CT can be extensively used in baked cookies as a rich source of polyphenols with strong antioxidant properties and high fibre content, as well as a fortification source for the development of functional foods. Full article
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<p>Radar plots of sensory evaluation of fresh cookies in different concentrations of CT.</p>
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<p>Overall acceptability of organoleptic characteristics of fresh cookies in different concentrations of CT.</p>
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<p>The appearance of cookies control, 2%, 4%, 6%, and 8% of CT flower.</p>
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<p>Total phenolics content and antioxidative activity with CT.</p>
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<p>Sensory profiling of control sample during 6 weeks of storage.</p>
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<p>Sensory profiling of sample with 6% CT flower during 6 weeks of storage.</p>
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11 pages, 578 KiB  
Article
Physico-Chemical Properties and Chemical Analysis of Wildflower Honey Before and After the Addition of Spirulina (Arthrospira platensis)
by Cosimo Taiti, Lara Costantini, Diego Comparini, Nicolò Merendino and Stefania Garzoli
Molecules 2024, 29(18), 4373; https://doi.org/10.3390/molecules29184373 - 14 Sep 2024
Viewed by 605
Abstract
In this study, in order to verify the effects due to the addition of spirulina (Arthrospira platensis) in a food product, a wildflower honey was analyzed in terms of chemical composition, physicochemical properties and antioxidant activity before and after the addition [...] Read more.
In this study, in order to verify the effects due to the addition of spirulina (Arthrospira platensis) in a food product, a wildflower honey was analyzed in terms of chemical composition, physicochemical properties and antioxidant activity before and after the addition of the spirulina. HS-SPME/GC–MS and HPLC/UV were applied to carry out the chemical analyses. The obtained results demonstrated that the volatile profile and also the sugar content were significantly influenced by the addition of spirulina, showing significant qualitative and quantitative differences compared to honey without spirulina. The increase in HMF in honey added with spirulina was significant, demonstrating that its presence could accelerate the Maillard reaction. Electrical conductivity measured by using a conductometer was also increased while the moisture content was reduced in honey enriched with spirulina. Instead, the pH value was similar between the two samples. On the other hand, honey fortification with spirulina determined a significant increase of 12.5% in the total phenolic content (TPC), and a 56.25% increase in protein content. Further, the total antioxidant capacity (TAC) was also evaluated and a significant increase was determined as a result of the addition of spirulina. In conclusion, honey enriched with A. platensis was found to be characterized by a high pool of bioactive metabolites as well as significant changes in almost all the measurements performed. Full article
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<p>Calibration curve of HMF.</p>
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17 pages, 1917 KiB  
Article
Cookies Fortified with Polyphenols Extracts: Impact on Phenolic Content, Antioxidant Activity, Inhibition of α-Amylase and α-Glucosidase Enzyme, Colour and Sensory Attractiveness
by Daria Pędziwiatr, Marina Cano Lamadrid and Aneta Wojdyło
Antioxidants 2024, 13(9), 1108; https://doi.org/10.3390/antiox13091108 - 13 Sep 2024
Viewed by 824
Abstract
The goal of the research was to determine the impact of fortification with polyphenolic compounds on (i) sensory attractiveness (global satisfaction, appearance, colour, odour, flavour, sweetness, bitterness), (ii) content of polyphenols and colour (L*, a*, b*) after the baking process and (iii) their [...] Read more.
The goal of the research was to determine the impact of fortification with polyphenolic compounds on (i) sensory attractiveness (global satisfaction, appearance, colour, odour, flavour, sweetness, bitterness), (ii) content of polyphenols and colour (L*, a*, b*) after the baking process and (iii) their bioactive potential (antioxidants activity and inhibiting of α-amylase and α-glucosidase enzyme). Fortification was made with extracts of polyphenolic compounds of selected plant raw materials rich in polyphenols from quince (fruits), tilia (flowers), pomegranate (skin), passion fruit (endocarp), sour cherries (leaves), haskap and chokeberry (berries), silver skin (coffee beans), rosehip (seeds). Depending on the nature of the polyphenol extract, flavan-3-ols (monomeric and polymeric), phenolic acid, flavonols and anthocyanins were identified in the product in amounts ranging from 53.7 to 212.6 mg/100 g DM. Cookies’ colour (L*, a*, b*) depended on the type of polyphenol extract used for fortification. Cookies with haskap, chokeberry and sour cherry presented the highest antioxidant potential. Cookies with chokeberry, haskap and rosehip presented high activity in inhibiting α-amylase (65.5, 60.6 and 62.2% of inhibition, respectively), but cookies with haskap, silver skin and quince in inhibiting α-glucosidase activity (23.0, 20.4 and 21.4% of inhibition, respectively). In the sensory evaluation, the most attractive were cookies with rosehip and pomegranate (6.3 and 5.8 score, respectively), but the lowest ratings were given to cookies with passion fruit and silver skin but especially quince cookies, which obtained the lowest desirability (3.7 score). The acceptability of fortified cookies was determined to the least extent by monomeric flavan-3-ols and phenolic acids (in minus in odour/flavour, bitterness, sweetness and global satisfaction), but anthocyanins, polymeric procyanidins and flavonols had the most significant positive impact on consumer acceptance of the assessed features, i.e., global satisfaction, odour/flavour, sweetness and bitterness (positive consumer drivers). Full article
(This article belongs to the Section Natural and Synthetic Antioxidants)
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<p>In the following graphs, the consumer acceptance of each attribute is shown (only statistically significant attributes are included). Cookie sample: 1—quince fruits, 2—tilia flowers, 3—pomegranate skin, 4—passionfruits endocarp, 5—sour cherry leaves, 6—haskap berry, 7—chokeberry, 8—silverskin, 9—roseship seeds.</p>
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<p>Analysis of the main components (PCA) biplot of fortified cookies and phenolic compounds, ABTS<sup>o+</sup>, FRAP and ORAC activity, antidiabetic activity and parametr L*a*b*.</p>
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<p>Dendrogram of agglomeration hierarchical clustering (AHC) for fortified cookies. Color line means the same clustering.</p>
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<p>Partial Least Squares Regression (Standardised coefficients. 95% Conf. Interv). Drivers of consumer acceptance of the developed samples. Reddish colour means negative correlation, while greenish colour means positive correlation.</p>
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<p>Preparation of cookies fortified with polyphenol extracts.</p>
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17 pages, 592 KiB  
Article
Effects of Replacing Cow’s Milk with Plant-Based Beverages on Potential Nutrient Intake in Sustainable Healthy Dietary Patterns: A Case Study
by Paola Biscotti, Massimiliano Tucci, Donato Angelino, Valentina Vinelli, Nicoletta Pellegrini, Cristian Del Bo’, Patrizia Riso and Daniela Martini
Nutrients 2024, 16(18), 3083; https://doi.org/10.3390/nu16183083 - 13 Sep 2024
Viewed by 950
Abstract
More consumers are replacing cow’s milk (CM) with plant-based drinks (PBD), but data indicating the nutritional impact are limited. This theoretical study aims to assess the effect of substituting CM with PBD sold in Italy on nutrient intake within two dietary patterns: one [...] Read more.
More consumers are replacing cow’s milk (CM) with plant-based drinks (PBD), but data indicating the nutritional impact are limited. This theoretical study aims to assess the effect of substituting CM with PBD sold in Italy on nutrient intake within two dietary patterns: one aligned with the EAT-Lancet Commission reference diet adapted to Italian food habits (EAT-IT) and another one in line with the Italian Dietary Guidelines (IDG). Nutrition information from 368 PBD were collected and categorized according to their descriptive name and their fortification or not with calcium (Ca- and nCa-fortified). The substitution of CM with each PBD category in both dietary patterns was conducted, and an analysis of nutrient content and adequacy was performed. Substituting CM with all PBD resulted in reduced protein intake, except for nCa-fortified soy drinks, decreased saturated fat and vitamins B2 and B12, and increased fiber intake. Replacing CM with nCa-fortified PBD within both diets decreased Ca intake. Following the substitution of CM with Ca-fortified PBD, variations in vitamin D intake depended on the PBD category. The main risk of nutritional inadequacy was observed in Ca and vitamin D levels, which may even be amplified considering the different bioavailability based on the source of nutrients. This study highlighted the important role of CM in meeting calcium requirements and the potential unintended consequences of substituting CM with PBD without considering their nutritional differences. Full article
(This article belongs to the Section Nutrition and Public Health)
27 pages, 3426 KiB  
Article
Combination of a Natural Bioactive Compound and High-Hydrostatic-Pressure Treatment to Increase the Shelf-Life and Quality of Pork Meat
by Tamás Csurka, Zoltán Fekete, Anna Visy, Karina Ilona Hidas, István Dalmadi, Ferenc Horváth, László Ferenc Friedrich and Gábor Jónás
Appl. Sci. 2024, 14(18), 8212; https://doi.org/10.3390/app14188212 - 12 Sep 2024
Viewed by 404
Abstract
Currently, there is a growing demand for ready-to-eat, prepared, high-quality, preservative-free products. However, the shelf-life of these products is often so short that a relatively high percentage of these products cannot be sold and end up as food waste. In this study, pork [...] Read more.
Currently, there is a growing demand for ready-to-eat, prepared, high-quality, preservative-free products. However, the shelf-life of these products is often so short that a relatively high percentage of these products cannot be sold and end up as food waste. In this study, pork loin with different marinades (paprika and herbs) was treated with different high hydrostatic pressures (0 MPa, 300 MPa, 450 MPa and 600 MPa) and a bioactive component (piperine) and then the quality of the meat was examined after 0, 4, 8, 12 and 14 days of cold storage. Changes were monitored using color, pH, texture and microbiological analyses. Both pressure, piperine enrichment, storage time and the interactions of different factors had a significant effect on the quality of the loin samples with different marinades. Due to the denaturation of myoglobin, meat slices were less red and lighter after HHP treatment. The addition of piperine reduced this lightness. The pH increased with increasing pressure and decreased with storage time. HHP treatment significantly increased meat hardness, with samples treated at 600 MPa being 19% harder than those treated at 450 MPa. Microbiological results indicated that HHP at 450 MPa and 600 MPa effectively reduced anaerobic total live plate counts, ensuring satisfactory sensory and microbiological quality throughout storage. Piperine fortification also resulted in a more favorable microbiological status during storage without any perceptible change in quality properties during storage. These findings underscore the effectiveness of HHP and piperine enrichment in enhancing the safety and quality of marinated meats. Full article
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<p>Anaerobic total live plate count [lg (CFU g<sup>−1</sup>)] results of paprika-marinated BA− meat sample groups.</p>
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<p>Anaerobic total live plate count [lg (CFU g<sup>−1</sup>)] results of paprika-marinated BA+ meat sample groups.</p>
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<p>Anaerobic total live plate count [lg (CFU g<sup>−1</sup>)] results of herb-marinated BA− meat sample groups.</p>
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<p>Anaerobic total live plate count [lg (CFU g<sup>−1</sup>)] results of herb-marinated BA+ meat sample groups.</p>
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22 pages, 864 KiB  
Article
Dietary Adherence to Recommendations among a Cohort of Adults and Teens with Celiac Disease Maintaining a Gluten-Free Diet Compared to a Nationally Representative Sample: A Cross-Sectional Study
by Jennifer W. Cadenhead, Anne R. Lee, Thanh Thanh T. Nguyen, Benjamin Lebwohl, Peter H. R. Green and Randi L. Wolf
Nutrients 2024, 16(18), 3067; https://doi.org/10.3390/nu16183067 - 11 Sep 2024
Viewed by 958
Abstract
Celiac disease (CeD) is a common autoimmune condition, with a prevalence of ~1%. Currently, a gluten-free diet (GFD) is the only treatment option. Due to fortification rules excluding gluten-free products in the United States of America (U.S.A.), understanding the nutritional adequacy of a [...] Read more.
Celiac disease (CeD) is a common autoimmune condition, with a prevalence of ~1%. Currently, a gluten-free diet (GFD) is the only treatment option. Due to fortification rules excluding gluten-free products in the United States of America (U.S.A.), understanding the nutritional adequacy of a GFD is important for promoting optimal health among those with CeD. Cross-sectional examination of multiple 24 h dietary recalls from a study sample of 50 adults and 30 teens with CeD was used to determine nutritional adequacy and excesses according to U.S.A. recommendations. The results were compared with those of 15,777 adults and 2296 teens from a nationally representative sample not reporting CeD, the National Health and Nutrition Examination Survey (NHANES) 2009–2014. Compared with NHANES, our study population was more at risk of low folate and carbohydrate (adults) consumption, and of excessive niacin and vitamin A (teens), as well as saturated and total fat consumption (adults). Overall, though, compared with NHANES, our study participants had similar nutrient concerns but fewer nutritional imbalances, with some notable exceptions. In addition to maintaining a GFD, individuals with CeD should be counseled to maintain a balanced diet and to pay attention to nutrient-dense foods. Special attention should be given to teens in providing dietary counseling to potentially mitigate the risk of future morbidity. Full article
(This article belongs to the Special Issue Recent Advances in Gluten-Free Diet and Celiac Disease)
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<p>Flow of study participant enrollment.</p>
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<p>Flow of NHANES participants.</p>
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12 pages, 279 KiB  
Article
Fortification of Goat Milk Yogurts with Encapsulated Postbiotic Active Lactococci
by Andrea Lauková, Marián Maďar, Natália Zábolyová, Aleksandra Troscianczyk and Monika Pogány Simonová
Life 2024, 14(9), 1147; https://doi.org/10.3390/life14091147 - 11 Sep 2024
Viewed by 484
Abstract
The species Lactococcus lactis is a bacterium extensively used in the dairy industry. This bacterium is Generally Recognized as Safe and was added to the European Food Safety Authority’s Qualified Presumption of Safety list. The major functions of this species in dairy fermentation [...] Read more.
The species Lactococcus lactis is a bacterium extensively used in the dairy industry. This bacterium is Generally Recognized as Safe and was added to the European Food Safety Authority’s Qualified Presumption of Safety list. The major functions of this species in dairy fermentation are the production of lactic acid from lactose, citric acid fermentation, and the hydrolysis of casein. But, the representatives of this species that produce bacteriocin substances can also exert an inhibitory effect against spoilage bacteria. The aims of this study were to test three lactococcal strains isolated from raw goat milk for their postbiotic activity and to test their stability in goat milk yogurts after their application in encapsulated form for their further application. To achieve these aims, validated methods were used. Three Lactococcus lactis strains (identified by Blastn 16S rRNA analysis) produced bacteriocin substances/postbiotics. These concentrated postbiotics inhibited the growth of enterococci and staphylococci (by up to 97.8%), reaching an inhibitory activity of up to 800 AU/mL. The encapsulated (freeze-dried) lactococci survived in the goat milk yogurts with sufficient stability. Strain MK2/8 fortified the yogurts in the highest amount (8.1 ± 0.0 cfu/g log 10). It did not influence the pH of the yogurt. Full article
(This article belongs to the Special Issue Food Microbiological Contamination)
15 pages, 627 KiB  
Article
Effect of Skimmed Milk Powder and Fruit Jams Addition on the Physicochemical Characteristics of Yogurt
by Simona Janoušek Honesová, Eva Samková, Eva Dadáková, Lucie Hasoňová, Markéta Jarošová, Karolína Reindl and Jan Bárta
Fermentation 2024, 10(9), 462; https://doi.org/10.3390/fermentation10090462 - 5 Sep 2024
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Abstract
In three consecutive experiments, natural yogurt (NY) and fruit yogurt (FY) fortified with 5 and 10% skimmed milk powder (SMP) and 10% jam from black currant (BC), elderberry (EB), and their mixture of 1:1 (BCEB) were analyzed, and consumer acceptance was assessed. In [...] Read more.
In three consecutive experiments, natural yogurt (NY) and fruit yogurt (FY) fortified with 5 and 10% skimmed milk powder (SMP) and 10% jam from black currant (BC), elderberry (EB), and their mixture of 1:1 (BCEB) were analyzed, and consumer acceptance was assessed. In Experiment 1, the effect of SMP (0, 5, and 10%) on selected physicochemical parameters of the NY was evaluated. With the increasing addition of SMP, a decrease in fat content (up to −19%) and, conversely, an increase in protein content (up to +82%) and viscosity were noted. Analyses of fruits and jams intended for yogurt fortification revealed a significantly higher vitamin C content in BC than in EB and higher anthocyanins in EB than in BC. In Experiment 2, NY with 5 and 10% SMP was fortified with 10% jams (BC or EB). A joint effect of SMP and the type of fruit jam was evaluated. The same trends in fat and protein contents as in Experiment 1 were detected. The sensory evaluation showed better acceptance of FY with 10% SMP and no differences between BC and EB perception. Thus, for Experiment 3, FY was prepared with only 10% SMP and 10% jam (BC, EB, BCEB). Significant differences were detected in active acidity and color measured in the CIELab system. These results were also confirmed in sensory evaluation. The overall acceptability showed that FY with different types of jam did not significantly differ. The launching of innovative fortified yogurt onto the market represents a promising way to increase the diversity of fermented dairy products with nutritionally desirable properties. Full article
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<p>Sensory profile of fruit yogurts enriched with 5 and 10% skimmed milk powder (SMP) and with 10% jam from black currant (10% BC) and elderberry (10% EB) evaluated by experienced panel—Experiment 2.</p>
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<p>Sensory profile of fruit yogurts enriched with 10% skimmed milk powder (SMP) and with 10% jam from black currant (BC), elderberry (EB), and BC and EB in a 1:1 ratio (BCEB) evaluated by experienced (<b>a</b>) and trained panels (<b>b</b>) Experiment 3.</p>
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