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Search Results (9,291)

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26 pages, 2925 KiB  
Review
Crops Disease Detection, from Leaves to Field: What We Can Expect from Artificial Intelligence
by Youssef Lebrini and Alicia Ayerdi Gotor
Agronomy 2024, 14(11), 2719; https://doi.org/10.3390/agronomy14112719 - 18 Nov 2024
Abstract
Agriculture is dealing with numerous challenges of increasing production while decreasing the amount of chemicals and fertilizers used. The intensification of agricultural systems has been linked to the use of these inputs which nevertheless have negative consequences for the environment. With new technologies, [...] Read more.
Agriculture is dealing with numerous challenges of increasing production while decreasing the amount of chemicals and fertilizers used. The intensification of agricultural systems has been linked to the use of these inputs which nevertheless have negative consequences for the environment. With new technologies, and progress in precision agriculture associated with decision support systems for farmers, the objective is to optimize their use. This review focused on the progress made in utilizing machine learning and remote sensing to detect and identify crop diseases that may help farmers to (i) choose the right treatment, the most adapted to a particular disease, (ii) treat diseases at early stages of contamination, and (iii) maybe in the future treat only where it is necessary or economically profitable. The state of the art has shown significant progress in the detection and identification of disease at the leaf scale in most of the cultivated species, but less progress is done in the detection of diseases at the field scale where the environment is complex and applied only in some field crops. Full article
(This article belongs to the Special Issue Innovations in Agriculture for Sustainable Agro-Systems)
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<p>Temporal dynamics of research trends in crop disease detection topics.</p>
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<p>Flowchart of the methodology for collecting data about plant disease identification using artificial intelligence and image data.</p>
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<p>Thematic clustering of the topics identified from the keywords as well as their temporal evolution based on data collected from 2000 to 2024. The node size of a topic is proportionate to its relative frequency.</p>
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<p>Thematic clustering of the topics identified from the keywords based on data collected from 2000 to 2024. The node size of a topic is proportionate to its relative frequency.</p>
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<p>Sankey diagram analysis of global research trends in crop disease detection using artificial intelligence and image processing for the period between 2000 and 2024.</p>
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25 pages, 14501 KiB  
Article
Root-Zone Salinity in Irrigated Arid Farmland: Revealing Driving Mechanisms of Dynamic Changes in China’s Manas River Basin over 20 Years
by Guang Yang, Xuejin Qiao, Qiang Zuo, Jianchu Shi, Xun Wu and Alon Ben-Gal
Remote Sens. 2024, 16(22), 4294; https://doi.org/10.3390/rs16224294 (registering DOI) - 18 Nov 2024
Viewed by 60
Abstract
The risk of soil salinization is prevalent in arid and semi-arid regions, posing a critical challenge to sustainable agriculture. This study addresses the need for accurate assessment of regional root-zone soil salt content (SSC) and understanding of underlying driving mechanisms, which [...] Read more.
The risk of soil salinization is prevalent in arid and semi-arid regions, posing a critical challenge to sustainable agriculture. This study addresses the need for accurate assessment of regional root-zone soil salt content (SSC) and understanding of underlying driving mechanisms, which are essential for developing effective salinization mitigation and water management strategies. A remote sensing inversion technique, initially proposed to estimate root-zone SSC in cotton fields, was adapted and validated more widely to non-cotton farmlands. Validation results (with a coefficient of determination R2 > 0.53) were obtained using data from a three-year (2020–2022) regional survey conducted in the arid Manas River Basin (MRB), Xinjiang, China. Based on this adapted technique, we analyzed the spatiotemporal distributions of root-zone SSC across all farmlands in MRB from 2001 to 2022. Findings showed that root-zone SSC decreased significantly from 5.47 to 3.77 g kg−1 over the past 20 years but experienced a slight increase of 0.15 g kg1 in recent five years (2017–2022), attributed to cultivated area expansion and reduced irrigation quotas due to local water shortages. The driving mechanisms behind root-zone SSC distributions were analyzed using an approach combined with two machine learning algorithms, eXtreme Gradient Boosting (XGBoost) and SHapley Additive exPlanation (SHAP), to identify influential factors and quantify their impacts. The approach demonstrated high predictive accuracy (R2 = 0.96 ± 0.01, root mean squared error RMSE = 0.19 ± 0.03 g kg1, maximum absolute error MAE = 0.14 ± 0.02 g kg1) in evaluating SSC drivers. Factors such as initial SSC, crop type distribution, duration of film mulched drip irrigation implementation, normalized difference vegetation index (NDVI), irrigation amount, and actual evapotranspiration (ETa), with mean (SHAP value) ≥ 0.02 g kg−1, were found to be more closely correlated with root-zone SSC variations than other factors. Decreased irrigation amount appeared as the primary driver for recent increased root-zone SSC, especially in the mid- and down-stream sections of MRB. Recommendations for secondary soil salinization risk reduction include regulation of the planting structure (crop choice and extent of planting area) and maintenance of a sufficient irrigation amount. Full article
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<p>Overview of the study area. The Manas River Basin was divided into 17 different partitions based on topography and irrigation zones, namely: north Xiayedi (North XYD), south Xiayedi (South XYD), north Mosuowan (North MSW), south Mosuowan (South MSW), north Xinhuzongchang (North XHZC), south Xinhuzongchang (South XHZC), north Anjihai (North AJH), south Anjihai (South AJH), north Jingouhe (North JGH), south Jingouhe (South JGH), north Shihezi (North SHZ), south Shihezi (South SHZ), north Manas (North MNS), south Manas (South MNS), Danangou (DNG), Ningjiahe (NJH), Qingshuihe (QSH).</p>
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<p>Layout of sampling points in the Manas River Basin from 2020 to 2022: (<b>a</b>) Location and land use distribution of irrigation zones in 2022 (the planting structure changed slightly from 2020 to 2022); (<b>b</b>) sampling point layout in AJH irrigation zone in 2020; (<b>c</b>) sampling point layout in MSW irrigation zone in 2020; (<b>d</b>) sampling point layout in AJH irrigation zone in 2021; (<b>e</b>) sampling point layout in MSW irrigation zone in 2021; (<b>f</b>) sampling point layout in DNG irrigation zone in 2021; (<b>g</b>) sampling point layout in North XYD irrigation zone in 2022; (<b>h</b>) sampling point layout in North SHZ irrigation zone in 2022.</p>
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<p>Comparisons between measured (<span class="html-italic">SSC<sub>measured</sub></span>) and fitted (<span class="html-italic">SSC<sub>fitted</sub></span>) or simulated (<span class="html-italic">SSC<sub>simulated</sub></span>) root-zone soil salt content of wheat fields in the Manas River Basin from 2020 to 2022: (<b>a</b>) 1:1 diagram; (<b>b</b>) Coefficient of determination (<span class="html-italic">R</span><sup>2</sup>), root mean squared error (<span class="html-italic">RMSE</span>), maximum absolute error (<span class="html-italic">MAE</span>).</p>
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<p>Comparisons between measured (<span class="html-italic">SSC</span><sub>measured</sub>) and fitted (<span class="html-italic">SSC</span><sub>fitted</sub>) or simulated (<span class="html-italic">SSC</span><sub>simulated</sub>) root-zone soil salt content of maize (and other minor crops) fields in the Manas River Basin from 2020 to 2022: (<b>a</b>) 1:1 diagram; (<b>b</b>) Coefficient of determination (<span class="html-italic">R</span><sup>2</sup>), root mean squared error (<span class="html-italic">RMSE</span>), maximum absolute error (<span class="html-italic">MAE</span>).</p>
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<p>Spatial distributions of root-zone soil salt content (<span class="html-italic">SSC</span>) and salinization classification categories during the peak growth stage of crops in the Manas River Basin in: (<b>a</b>) 2002; (<b>b</b>) 2007; (<b>c</b>) 2011; (<b>d</b>) 2017; (<b>e</b>) 2022.</p>
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<p>Changes in root-zone soil salt content (<span class="html-italic">SSC</span>) and areas of different categories of salinized soil in the Manas River Basin from 2001 to 2022.</p>
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<p>SHAP bar plot (<b>a</b>) and summary plot (<b>b</b>) of the XGBoost model trained based on different factors affecting root-zone soil salt content in the Manas River Basin.</p>
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<p>SHAP dependence plot of the top seven influencing factors with mean (<math display="inline"><semantics> <mrow> <mfenced close="|" open="|"> <mrow> <mrow> <mi>SHAP</mi> <mo> </mo> <mi>value</mi> </mrow> </mrow> </mfenced> </mrow> </semantics></math>) ≥ 0.02 g kg<sup>−1</sup>: (<b>a</b>) Initial <span class="html-italic">SSC</span>; (<b>b</b>) CFP; (<b>c</b>) MFP; (<b>d</b>) IPF; (<b>e</b>) NDVI; (<b>f</b>) irrigation; (<b>g</b>) <span class="html-italic">TET<sub>a</sub></span>.</p>
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<p>SHAP waterfall plots of influencing factors in the partitions of upstream mountain DNG (<b>a</b>,<b>e</b>,<b>i</b>), upstream piedmont plain South AJH (<b>b</b>,<b>f</b>,<b>j</b>), midstream oasis plain North AJH (<b>c</b>,<b>g</b>,<b>k</b>) and downstream oasis–desert transition North XYD (<b>d</b>,<b>h</b>,<b>l</b>) in 2002 (<b>a</b>–<b>d</b>), 2011 (<b>e</b>–<b>h</b>) and 2022 (<b>i</b>–<b>l</b>). Red columns are positive SHAP values and blue columns negative.</p>
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19 pages, 7140 KiB  
Article
Design of a Contact-Type Electrostatic Spray Boom System Based on Rod-Plate Electrode Structure and Field Experiments on Droplet Deposition Distribution
by Hao Sun, Changxi Liu, Yufei Li, Hang Shi, Shengxue Zhao, Miao Wu and Jun Hu
Agronomy 2024, 14(11), 2715; https://doi.org/10.3390/agronomy14112715 - 18 Nov 2024
Viewed by 100
Abstract
Spraying is currently one of the main methods of pesticide application worldwide. It converts the pesticide solution into fine droplets through a sprayer, which then deposit onto target plants. Therefore, in the process of pesticide application, improving the effectiveness of spraying while minimizing [...] Read more.
Spraying is currently one of the main methods of pesticide application worldwide. It converts the pesticide solution into fine droplets through a sprayer, which then deposit onto target plants. Therefore, in the process of pesticide application, improving the effectiveness of spraying while minimizing or preventing crop damage has become a key issue. Combining the advantages of electrostatic spraying technology with the characteristics of ground boom sprayers, a contact-type electrostatic boom spraying system based on a rod–plate electrode structure was designed and tested on a self-propelled boom sprayer. The charging chamber was designed based on the characteristics of the rod–plate electrode and theoretical analysis. The reliability of the device was verified through COMSOL numerical simulations, charge-to-mass ratio, droplet size, and droplet size spectrum measurements, and a droplet size prediction model was established. The deposition characteristics in soybean fields were analyzed using the Box–Behnken experimental design method. The results showed that the rod–plate electrode structure demonstrated its superiority with a maximum spatial electric field of 2.31 × 106 V/m. When the spray pressure was 0.3 MPa and the charging voltage was 8 kV, the droplet size decreased by 26.6%, and the charge-to-mass ratio reached 2.88 mC/kg. Field experiments showed that when the charging voltage was 8 kV, the spray pressure was 0.3 MPa, the traveling speed was 7 km/h, and the number of deposited droplets was 8517. This study provides some basis for the application of electrostatic spraying technology in large-scale field operations. Full article
(This article belongs to the Section Agricultural Biosystem and Biological Engineering)
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<p>Contact electrostatic spray system composition diagram.</p>
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<p>Cross-section diagram of contact charging compartment.</p>
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<p>Charging bin physical picture.</p>
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<p>Schematic diagram of working principle.</p>
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<p>Schematic diagram of working principle.</p>
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<p>Selected electrostatic generator and power supply.</p>
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<p>Droplet size test site.</p>
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<p>Schematic layout of test points.</p>
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<p>Field work drawing.</p>
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<p>Schematic layout of water-sensitive paper.</p>
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<p>Schematic layout of water-sensitive paper.</p>
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<p>Spatial electric field simulation of different number of electrodes.</p>
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<p>The proportion of the electric field strength under different numbers of cylindrical electrodes.</p>
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<p>A cloud diagram of local electric field lines in the xy section of the fifth electrode.</p>
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<p>Diameter of droplet volume at different charging voltages.</p>
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<p>Scatter plot of measured values and predicted values.</p>
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<p>Spatial distribution of conventional and electrostatic droplet sizes.</p>
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<p>Response surface of interaction factors influencing test indexes.</p>
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17 pages, 3571 KiB  
Article
Geospatially Informed Water Pricing for Sustainability: A Mixed Methods Approach to the Increasing Block Tariff Model for Groundwater Management in Arid Regions of Northwest Bangladesh
by Ragib Mahmood Shuvo, Radwan Rahman Chowdhury, Sanchoy Chakroborty, Anutosh Das, Abdulla Al Kafy, Hamad Ahmed Altuwaijri and Muhammad Tauhidur Rahman
Water 2024, 16(22), 3298; https://doi.org/10.3390/w16223298 (registering DOI) - 17 Nov 2024
Viewed by 296
Abstract
Groundwater depletion in arid regions poses a significant threat to agricultural sustainability and rural livelihoods. This study employs geospatial analysis and economic modeling to address groundwater depletion in the arid Barind region of Northwest Bangladesh, where 84% of the rural population depends on [...] Read more.
Groundwater depletion in arid regions poses a significant threat to agricultural sustainability and rural livelihoods. This study employs geospatial analysis and economic modeling to address groundwater depletion in the arid Barind region of Northwest Bangladesh, where 84% of the rural population depends on agriculture. Using remote sensing and GIS, we developed an elevation map revealing areas up to 60 m above sea level, exacerbating evaporation and aquifer dryness. Field data collected through Participatory Rural Appraisal tools showed farmers exhibiting “ignorant myopic” behavior, prioritizing short-term profits over resource conservation. To address this, an Increasing Block Tariff (IBT) water pricing model was developed, dividing water usage into three blocks based on irrigation hours: 1–275 h, 276–550 h, and 551+ h. The proposed IBT model significantly increases water prices across the three blocks: 117 BDT/hour for the first block (from current 100–110 BDT/hour), 120 BDT/hour for the second block, and 138 BDT/hour for the third block. A demand function (y = −0.1178x + 241.8) was formulated to evaluate the model’s impact. The results show potential reductions in groundwater consumption: 59 h in the first block, 26 h in the second block, and 158 h in the third block. These reductions align with the principles of integrated water resource management (IWRM): social equity, economic efficiency, and environmental integration. The model incorporates economic externalities (e.g., well lifting costs) and environmental externalities (e.g., crop pattern shifts), with total costs reaching 92,709,049 BDT for environmental factors. This research provides a framework for sustainable groundwater management in arid regions, potentially reducing overextraction while maintaining agricultural productivity. The proposed IBT model offers a locally driven solution to balance resource conservation with the livelihood needs of farming communities in the Barind tract. By combining remote sensing, GIS, and economic modeling, this research provides a framework for sustainable groundwater management in arid regions, demonstrating the power of geospatial technologies in addressing complex water resource challenges. Full article
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<p>Study area map [<a href="#B17-water-16-03298" class="html-bibr">17</a>].</p>
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<p>(<b>a</b>) Left: elevation map and (<b>b</b>) right: groundwater fluctuation map of the study area.</p>
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<p>Distribution of farmland cultivated by the farmers.</p>
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<p>Calculated reduction in consumption hours of irrigation with the help of proposed water pricing model: (<b>a</b>) first block; (<b>b</b>) second block; (<b>c</b>) third block.</p>
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<p>Change in consumption of irrigation water (m<sup>3</sup>/hour) with the help of the proposed model.</p>
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18 pages, 3462 KiB  
Article
Evaluating Physiological and Yield Indices of Egyptian Barley Cultivars Under Drought Stress Conditions
by Wessam A. Abdelrady, Elsayed E. Elshawy, Hassan A. Abdelrahman, Syed Muhammad Hassan Askri, Zakir Ibrahim, Mohamed Mansour, Ibrahim S. El-Degwy, Taha Ghazy, Aziza A. Aboulila and Imran Haider Shamsi
Agronomy 2024, 14(11), 2711; https://doi.org/10.3390/agronomy14112711 - 17 Nov 2024
Viewed by 216
Abstract
Climate change significantly threatens crops, mainly through drought stress, affecting barley, which is essential for food and feed globally. Ten barley cultivars were evaluated under normal and drought stress conditions during the 2019/20 and 2020/21 seasons, focusing on traits such as days to [...] Read more.
Climate change significantly threatens crops, mainly through drought stress, affecting barley, which is essential for food and feed globally. Ten barley cultivars were evaluated under normal and drought stress conditions during the 2019/20 and 2020/21 seasons, focusing on traits such as days to heading and maturity, plant height, number of spikes m−2, spike length, 1000-kernel weight, and biological and grain yield. Drought stress significantly reduced most of these traits. The genotypes showed significant differences in their responses to irrigation treatments, with the interaction between seasons and cultivars also being significant for most traits. The grain yield and 1000-kernel weight were among the least affected traits under drought stress, respectively. Notably, Giza138 and Giza126 showed strong drought tolerance, suitable for drought-resilient breeding. In season one, Giza126, Giza134, and Giza138 yielded 13%, 9%, and 11%, respectively, while Giza135 and Giza129 showed higher reductions at 31% and 39%. In season two, Giza126, Giza134, and Giza138 had reductions of 14%, 10%, and 13%, respectively, while Giza135 and Giza129 again exhibited higher reductions at 31% and 38%. These cultivars also showed strong performance across various stress tolerance indices, including the MP, YSI, STI, GMP, and YI. Giza 134 demonstrated the lowest values for the SDI and TOL, indicating superior drought stress tolerance. On the other hand, Giza 129 and Giza 135 were the most sensitive to drought stress, experiencing significant reductions across critical traits, including 6.1% in days to heading, 18.37% in plant height, 28.21% in number of kernel spikes−1, 38.45% in grain yield, and 34.91% in biological yield. In contrast, Giza 138 and Giza 2000 showed better resilience, with lower reductions in the 1000-kernel weight (6.41%) and grain yield (10.61%), making them more suitable for drought-prone conditions. Giza 126 and Giza 132 also exhibited lower sensitivity, with minimal reductions in days to heading (2%) and maturity (2.4%), suggesting potential adaptability to water-limited environments. Giza 126 maintained the highest root lengths and had the highest stomatal conductance. Giza 138 consistently had the highest chlorophyll content, with SPAD values decreasing to 79% under drought. Despite leading in shoot length, Giza 135 decreased to 42.59% under drought stress. In conclusion, Giza 126 and Giza 138 showed adaptability to water-limited conditions with minimal impact on phenological traits. Giza 126 had the longest roots and highest stomatal conductance, while Giza 138 consistently maintained a high chlorophyll content. Together, they and Giza 134 are valuable for breeding programs to improve barley drought tolerance. Full article
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Graphical abstract
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<p>Comparative analysis of ten cultivars under varying drought conditions. The figure illustrates the impact of normal conditions, moderate drought stress, and severe drought stress on the growth and root development of different Giza cultivars, highlighting the varying levels of drought tolerance across cultivars.</p>
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<p>Performance and drought tolerance of ten cultivars for shoot length (cm) (<b>a</b>), root length (cm) (<b>b</b>), fresh shoot weight (<b>c</b>), and shoot dry weight (<b>d</b>). Different letters indicated significant variations among the cultivars using LSD 0.05.</p>
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<p>Photosynthetic efficiency, Photosystem II quantum efficiency, stomatal conductance, and SPAD values of ten Giza cultivars under normal, moderate, and severe drought conditions. (<b>a</b>,<b>b</b>) show the photosynthetic efficiency and Photosystem II quantum efficiency percentages; (<b>c</b>) the stomatal conductance under drought stress (gsw) values; (<b>d</b>) SPAD values under different drought stress. Different letters indicated significant variations among the cultivars using LSD 0.05.</p>
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<p>Mean performance of days to heading (<b>a</b>) and number of days to maturity (<b>b</b>), plant height (cm) (<b>c</b>), spike length (cm) (<b>d</b>), and number of kernel spikes<sup>−1</sup> (<b>e</b>) for studied cultivars under normal and drought stress conditions across irrigation treatments and two seasons. Different letters indicated significant variations among the cultivars using LSD 0.05.</p>
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<p>Performance of the number of kernel spikes<sup>−1</sup> (<b>a</b>), 1000-kernel weight (<b>b</b>), biological yield ha<sup>−1</sup> (<b>c</b>), and grain yield ha<sup>−1</sup> (<b>d</b>) for the studied cultivars under normal and drought stress conditions in the two seasons across the irrigation treatments and the two seasons. Different letters indicated significant variations among the cultivars using LSD 0.05.</p>
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<p>Grain yield performance and stability of ten cultivars under normal and drought stress across two seasons. A GGE biplot was used to rank 10 cultivars (G1–G10: Giza 123, Giza 126, Giza 132, Giza 134, Giza 130, Giza 136, Giza 138, Giza 2000, Giza 135, Giza 129) for grain yield across four environments: E1 (normal, 2019/20), E2 (drought stress, 2019/20), E3 (normal, 2020/21), and E4 (drought stress, 2020/21). The Average Environment Axis (AEA) indicated higher mean performance, while its perpendicular axis indicated greater variability or instability. The analysis highlighted yield performance and stability differences under normal and drought conditions.</p>
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21 pages, 3045 KiB  
Article
Natural and Organic Input-Based Integrated Nutrient-Management Practices Enhance the Productivity and Soil Quality Index of Rice–Mustard–Green Gram Cropping System
by Sukamal Sarkar, Anannya Dhar, Saikat Dey, Sujan Kr. Chatterjee, Shibasis Mukherjee, Argha Chakraborty, Gautam Chatterjee, Natesan Ravisankar and Mohammed Mainuddin
Land 2024, 13(11), 1933; https://doi.org/10.3390/land13111933 - 17 Nov 2024
Viewed by 291
Abstract
The effects of integrated nutrient-management (INM) practices on soil quality are essential for sustaining agro-ecosystem productivity. The soil quality index (SQI) serves as a tool to assess the physical, chemical, and biological potential of soils as influenced by various edaphic and agronomic practices. [...] Read more.
The effects of integrated nutrient-management (INM) practices on soil quality are essential for sustaining agro-ecosystem productivity. The soil quality index (SQI) serves as a tool to assess the physical, chemical, and biological potential of soils as influenced by various edaphic and agronomic practices. A multiyear (2018–2021) field experiment was performed at the University Organic Research Farm, Narendrapur, West Bengal, India, to investigate the influence of integrated and sole applications of different conventional fertilizers, organic (e.g., vermicompost), and natural farming inputs (e.g., Dhrava Jeevamrit and Ghana Jeevamrit) on SQIs and crop productivity of rice–mustard–green gram-based cropping systems. A total of 12 parameters were selected for the assessment of SQI, amongst which only four, namely pH, organic carbon %, total actinomycetes, and bulk density, were retained for the minimum data set based on principal component analysis (PCA). In this study, the maximum SQI value (0.901) of the experimental soil was recorded in the INM practice of 25% organic and 25% inorganic nutrient inputs, and the rest with natural farming inputs, which augments the SQI by 24% compared to the 100% inorganic nutrient treatment. Amongst the different soil parameters, the highest contribution was from the pH (35.18%), followed by organic carbon % (26.77%), total actinomycetes (10.95%), and bulk density (6.98%). The yields in different cropping systems varied year-wise across treatments. Notably, the highest yield in rainy rice was estimated in the 100% organic treatment, followed by INM practices in the subsequent years, and finally, the combination of organic and natural inputs in the final year. In the case of mustard, the combination of organic and natural inputs resulted in the highest productivity in the initial and last years of study, while the 100% organic treatment resulted in higher productivity in subsequent years. Green gram showed a dynamic shift in yield between the 100% organic and integrated treatments over the years. Further, a strong correlation was also established between the soil physico-chemical parameters and the SQI. Overall, this study concludes that the natural and organic input-based INM practice enhances the soil quality and crop productivity of the rice–mustard–green gram cropping system under the coastal saline zone. Full article
(This article belongs to the Special Issue Ecosystem Disturbances and Soil Properties)
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<p>Location map of the experimental site (The map was prepared with QGIS Open-source Software, not for commercial use).</p>
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<p>Metrological parameters (<b>a</b>) maximum minimum temperature, minimum temperature, and rainfall; (<b>b</b>) relative humidity and sunshine hour of the experimental period (2018–2022).</p>
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<p>Balance of (<b>a</b>) soil organic carbon, (<b>b</b>) available nitrogen, (<b>c</b>) available phosphorus, and (<b>d</b>) available potassium in post-harvest soil compared to initial soil. Values in parentheses represent the increase (+) or decrease (−) from the initial value (before starting the experiment). [NM1:100% organic inputs, NM2:50% organic inputs + natural inputs, NM3: 50% organic inputs + 50% inorganic inputs, NM4: 25% organic inputs + 25% inorganic inputs + natural inputs, NM5:100% inorganic inputs].</p>
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<p>Dynamics of (<b>a</b>) total bacteria, (<b>b</b>) total fungi, and (<b>c</b>) total actinomycetes population in pre- and post-experimental soil. Values in parentheses represent the increase (+) or decrease (−) from the initial value (before starting the experiment). [NM1:100% organic inputs, NM2:50% organic inputs + natural inputs, NM3: 50% organic inputs + 50% inorganic inputs, NM4: 25% organic inputs + 25% inorganic inputs + natural inputs, NM5:100% inorganic inputs]. Box column followed by different letters is significantly different by Duncan’s Multiple Range Test (DMRT) at <span class="html-italic">p</span> ≤ 0.05.</p>
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<p>Soil quality index and contribution of soil quality indicator for different integrated nutrient-management practices. Values in parentheses represent the increase (+) or decrease (−) from the initial value (before starting the experiment). [NM1:100 % organic inputs, NM2:50 % organic inputs + natural inputs, NM3: 50% organic inputs + 50% inorganic inputs, NM4: 25% organic inputs + 25% inorganic inputs + natural inputs, NM5:100% inorganic inputs]. Vertical column followed by different letters is significantly different by Duncan’s Multiple Range Test (DMRT) at <span class="html-italic">p</span> ≤ 0.05.</p>
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<p>Pearson’s correlation matrix of all soil quality indicating factors.</p>
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<p>Relationship between soil quality index (SQI) and (<b>a</b>) Bulk density/cc, (<b>b</b>) Soil pH, (<b>c</b>) organic carbon, and (<b>d</b>) Total actinomycetes (×10<sup>6</sup> cfu/gm) [Abbreviation: *** correlation is significant at 0.001 level, ** correlation is significant at 0.01 level, * correlation is significant at <span class="html-italic">p</span> ≤ 0.05 level].</p>
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15 pages, 747 KiB  
Article
Promoting the Economic Sustainability of Small-Scale Farmers Through Versatile Machinery in the Republic of Korea
by Seokho Kang, Haesung Jung, Seunggwi Kwon, Youngyoon Jang, Seungmin Woo and Yushin Ha
Sustainability 2024, 16(22), 10022; https://doi.org/10.3390/su162210022 - 17 Nov 2024
Viewed by 318
Abstract
The increasing use of tractors and implements is replacing manual labor, but adds financial burdens on small-scale farmers due to rising costs. Many farmers have turned to leasing and renting machinery to mitigate these expenses, while repair and maintenance costs remain significant. Government [...] Read more.
The increasing use of tractors and implements is replacing manual labor, but adds financial burdens on small-scale farmers due to rising costs. Many farmers have turned to leasing and renting machinery to mitigate these expenses, while repair and maintenance costs remain significant. Government interventions aim to alleviate these burdens, but income disparities between urban and rural areas persist, and the impact of machinery use on climate change and the environment poses further challenges. Strategies like omitting some operation steps and adopting versatile machinery are proposed to cut costs and promote economic sustainability for small-scale farmers. Therefore, this study assessed the economic benefits of using versatile machinery in farming, especially for small-scale rural farmers. Farming processes were divided into field preparation and crop season activities. Field preparation included rotary tillage, ridge formation, and mulching, whereas crop season activities included harvesting and transportation. Annual usage and production cost analyses per hectare, including labor, fuel, and interest, alongside purchasing cost surveys, were conducted. Versatile machinery reduced annual usage costs for field preparation and crop season activities by 63.54% and 71.71%, respectively. This effect was more pronounced for farms under 2 ha, especially those employing manual harvest and transportation. Small-scale farmers, such as those cultivating hot pepper farms, are strongly encouraged to adopt versatile machinery to mitigate expenses and labor costs. The significance of adopting studied methodology will be amplified with the rising cost of labor. Consequently, utilization of versatile machinery in field farming for small-scale farms is projected to increase incomes not through enhanced production, but by significantly reducing the annual usage costs associated with agricultural machinery. This approach not only alleviates financial burdens but also enhances the sustainability of farm management, ensuring long-term viability and environmental stewardship. Full article
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<p>The conventional and modified farming processes for upland field farming.</p>
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<p>Comparison of production costs using Integrated_2 (<b>a</b>) vs. Conventional method; (<b>b</b>) vs. labor.</p>
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26 pages, 869 KiB  
Article
Integrating Morpho-Physiological, Biochemical, and Molecular Genotyping for Selection of Drought-Tolerant Pigeon Pea (Cajanus cajan L.) Genotypes at Seedling Stage
by Benjamin O. Ouma, Kenneth Mburu, Geoffrey K. Kirui, Edward K. Muge and Evans N. Nyaboga
Plants 2024, 13(22), 3228; https://doi.org/10.3390/plants13223228 (registering DOI) - 16 Nov 2024
Viewed by 500
Abstract
Pigeon pea (Cajanus cajan (L.) Millsp.), a potential legume as an economic source of protein, is commonly cultivated in tropical and subtropical regions of the world. It possesses medicinal properties and acts as a cash crop, benefiting low-income farmers economically. The identification [...] Read more.
Pigeon pea (Cajanus cajan (L.) Millsp.), a potential legume as an economic source of protein, is commonly cultivated in tropical and subtropical regions of the world. It possesses medicinal properties and acts as a cash crop, benefiting low-income farmers economically. The identification of pigeon peas exhibiting drought tolerance has become crucial in addressing water scarcity issues in the agriculture sector. In addition, exploring the genetic diversity among genotypes is important for conservation, management of genetic resources, and breeding programs. The aim of this study was to evaluate the morpho-physiological and biochemical responses of selected pigeon pea genotypes under pot-induced water stress conditions through different field capacities as well as the genetic diversity using start codon targeted (SCoT) markers. A significant variation was observed for the physiological traits studied. The accumulation of fresh weight (FW) and dry weight (DW) was significantly reduced in moderate and severe drought stress conditions. The lowest % DW decrease was found in LM (35.39%), KAT (39.43%), and SM (46.98%) than other genotypes at severe drought stress. Analyses of physiological responses including the photosynthetic efficiency (Phi2), the chlorophyll content (SPAD), and the relative water content (RWC) revealed positive and negative correlations with various parameters, reflecting the impact of drought stress on the chlorophyll content. The results revealed that biochemical traits including the total phenolic content, soluble sugars, proline, total protein, total amino acids, and free amino acids were variably and significantly increased under water stress. Antioxidant enzyme activity levels, specifically ascorbate peroxidase (APX) and catalase, varied among the genotypes and in response to severe water stress, offering further insights into adaptive responses. The eight genotypes analysed by use of 20 SCoT markers revealed 206 alleles and an average of 10.3 alleles per locus. Genetic similarity ranged from 0.336 to 0.676, clustering the pigeon pea genotypes into two major groups by the unweighted pair group method of arithmetic averages (UPGMA) cluster analysis. Principal coordinate analysis (PCoA) explained 43.11% of genetic variation and based on analysis of molecular variance, a high genetic variation (80%) within populations was observed, emphasizing the potential for genetic improvement. Among the eight genotypes studied, LM and KAT were drought tolerant and genetically diverse and therefore could be used as parents for developing drought tolerance in pigeon pea. Full article
(This article belongs to the Section Plant Response to Abiotic Stress and Climate Change)
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<p>Unweighted pair group method of arithmetic mean (UPGMA) dendrogram based on Jaccard’s coefficient pigeon pea genotypes using SCoT markers. Genotypes: P1, P2, P3, SM, MM, LM, KAT, and P9.</p>
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<p>Principal coordinate analysis (PCoA) of the eight pigeon pea genotypes collected from Genebank of Kenya (Kitui and Machakos Counties) as revealed by the 20 SCoT markers.</p>
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16 pages, 29569 KiB  
Article
Assessing Weed Canopy Cover in No-Till and Conventional Tillage Plots in Winter Wheat Production Using Drone Data
by Judith N. Oppong, Clement E. Akumu and Sam Dennis
Agronomy 2024, 14(11), 2706; https://doi.org/10.3390/agronomy14112706 - 16 Nov 2024
Viewed by 413
Abstract
Weed canopy cover assessment, particularly using drone-acquired data, plays a vital role in precision agriculture by providing accurate, timely, and spatially detailed information, enhancing weed management decision-making in response to environmental and management variables. Despite the significance of this approach, few studies have [...] Read more.
Weed canopy cover assessment, particularly using drone-acquired data, plays a vital role in precision agriculture by providing accurate, timely, and spatially detailed information, enhancing weed management decision-making in response to environmental and management variables. Despite the significance of this approach, few studies have investigated weed canopy cover through drone-based imagery. This study aimed to fill this gap by evaluating the effects of conventional tillage (CT) and no-till (NT) practices on weed canopy cover in a winter wheat field over two growing seasons. Results indicated that in the 2022–2023 season, weed populations were similar between tillage systems, with a high mean weed cover of 1.448 cm2 ± 0.241 in CT plots. In contrast, during the 2023–2024 season, NT plots exhibited a substantially higher mean weed cover (1.784 cm2 ± 0.167), with a significant overall variation (p < 0.05) in weed distribution between CT and NT plots. These differences suggest that, while CT practices initially mask weed emergence by burying seeds and disrupting root systems, NT practices encourage greater weed establishment over time by leaving seeds near the soil surface. These findings provide valuable insights for optimizing weed management practices, emphasizing the importance of comprehensive approaches to improve weed control and overall crop productivity. Full article
(This article belongs to the Special Issue Weed Ecology, Evolution and Management)
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<p>Location of the study area with an insert of the study field.</p>
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<p>Schematic illustration of the methodology used for this study.</p>
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<p>No-till and conventional tillage plot layout for winter wheat production.</p>
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<p>West-to-east drone flight path for field image acquisition.</p>
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<p>Classified weed canopy cover map derived from the 2022–2023 tillering growth stage.</p>
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<p>Classified weed canopy cover map derived from the 2022–2023 jointing growth stage.</p>
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<p>Classified weed canopy cover map derived from the 2022–2023 booting growth stage.</p>
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<p>Classified weed canopy cover map derived from the 2022–2023 mature growth stage.</p>
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<p>Classified weed canopy cover map derived from the 2023–2024 tillering growth stage.</p>
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<p>Classified weed canopy cover map derived from the 2023–2024 jointing growth stage.</p>
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<p>Classified weed canopy cover map derived from the 2023–2024 booting growth stage.</p>
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<p>Classified weed canopy cover map derived from the 2023–2024 mature growth stage.</p>
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<p>Mean canopy cover of weeds for conventional tillage and no-till over the study period. Error bars = standard error of mean (SE).</p>
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20 pages, 4501 KiB  
Article
Study on Path Planning in Cotton Fields Based on Prior Navigation Information
by Meng Wang, Changhe Niu, Zifan Wang, Yongxin Jiang, Jianming Jian and Xiuying Tang
Agriculture 2024, 14(11), 2067; https://doi.org/10.3390/agriculture14112067 - 16 Nov 2024
Viewed by 294
Abstract
Aiming at the operation scenario of existing crop coverage and the need for precise row alignment, the sowing prior navigation information of cotton fields in Xinjiang was used as the basis for the study of path planning for subsequent operations to improve the [...] Read more.
Aiming at the operation scenario of existing crop coverage and the need for precise row alignment, the sowing prior navigation information of cotton fields in Xinjiang was used as the basis for the study of path planning for subsequent operations to improve the planning quality and operation accuracy. Firstly, the characteristics of typical turnaround methods were analyzed, the turnaround strategy for dividing planning units was proposed, and the horizontal and vertical operation connection methods were put forward. Secondly, the obstacle avoidance strategies were determined according to the traits of obstacles. The circular arc–linear and cubic spline curve obstacle avoidance path generation methods were proposed. Considering the dual attributes of walking and the operation of agricultural machinery, four kinds of operation semantic points were embedded into the path. Finally, path generation software was designed. The simulation and field test results indicated that the operation coverage ratio CR ≥ 98.21% positively correlated with the plot area and the operation distance ratio DR ≥ 86.89% when non-essential reversing and obstacles were ignored. CR and DR were negatively correlated with the number of obstacles when considering obstacles. When considering non-essential reversing, the full coverage of operating rows could be achieved, but DR would be reduced correspondingly. Full article
(This article belongs to the Section Agricultural Technology)
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<p>Example of navigation paths for cotton mulching sowing.</p>
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<p>Schematic diagram of wide–narrow row cotton planting.</p>
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<p>The overall design process of prior semantic map. (<b>a</b>) Planning process; (<b>b</b>) schematic of planning results.</p>
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<p>Commonly used turnaround paths for agricultural machinery.</p>
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<p>RSR and LSR paths of Dubins curves.</p>
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<p>Schematic diagram of dividing the planning unit with nesting operation. (<b>a</b>) Schematic diagram of dividing the planning unit; (<b>b</b>) examples of traversal effect when <span class="html-italic">n<sub>uint</sub></span> takes odd and even numbers.</p>
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<p>Schematic diagram of the bow-turn path.</p>
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<p>Schematic diagram of the fishtail-turn path: (<b>a</b>) vertical; (<b>b</b>) horizontal.</p>
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<p>Schematic diagram of the horizontal and vertical operation connection path. (<b>a</b>) Backward–Arc; (<b>b</b>) Backward–Arc–Backward; (<b>c</b>) application examples.</p>
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<p>Schematic diagram of obstacle avoidance path.</p>
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<p>Schematic diagram of circular arc–linear obstacle avoidance path. (<b>a</b>) Arc–semicircle–arc; (<b>b</b>) arc–arc–line–arc–arc; (<b>c</b>) arc–line–semicircle–line–arc; (<b>d</b>) arc–line–arc–line–arc–line–arc.</p>
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<p>Schematic diagram of obstacle avoidance requirements.</p>
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<p>Example of avoidance path generation.</p>
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<p>Schematic diagram of embedding semantics.</p>
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<p>Path generation software interface (Version 1.0).</p>
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<p>Examples of simulation paths without considering lateral operations. (<b>a</b>) Clockwise operation; (<b>b</b>) counterclockwise operation. Note: The blank area in the operation line indicates the obstacle area (the same as below).</p>
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<p>Simulation path considering the lateral operation: (<b>a</b>) when there is sufficient horizontal turnaround area; (<b>b</b>) considering horizontal turnaround area constraints.</p>
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<p>Planning path of plot A.</p>
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<p>Planning paths of plots B<sub>1</sub> and B<sub>2</sub>: (<b>a</b>) navigation paths; (<b>b</b>) planned path.</p>
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16 pages, 2408 KiB  
Article
Adaptive Agronomic Strategies for Enhancing Cereal Yield Resilience Under Changing Climate in Poland
by Elżbieta Wójcik-Gront, Dariusz Gozdowski, Rafał Pudełko and Tomasz Lenartowicz
Agronomy 2024, 14(11), 2702; https://doi.org/10.3390/agronomy14112702 - 16 Nov 2024
Viewed by 193
Abstract
Climate-driven changes have raised concerns about their long-term impacts on the yield resilience of cereal crops. This issue is critical in Poland as it affects major cereal crops like winter triticale, spring wheat, winter wheat, spring barley, and winter barley. This study investigates [...] Read more.
Climate-driven changes have raised concerns about their long-term impacts on the yield resilience of cereal crops. This issue is critical in Poland as it affects major cereal crops like winter triticale, spring wheat, winter wheat, spring barley, and winter barley. This study investigates how soil nutrient profiles, fertilization practices, and crop management conditions influence the yield resilience of key cereal crops over a thirteen-year period (2009–2022) in the context of changing climate expressed as varying Climatic Water Balance. Data from 47 locations provided by the Research Centre for Cultivar Testing were analyzed to assess the combined effects of agronomic practices and climate-related water availability on crop performance. Yield outcomes under moderate and enhanced management practices were contrasted using Classification and Regression Trees to evaluate the relationships between yield variations and agronomic factors, including soil pH, nitrogen, phosphorus, potassium fertilization, and levels of phosphorus, potassium, and magnesium in the soil. The study found a downward trend in Climatic Water Balance, highlighting the increasing influence of climate change on regional water resources. Crop yields responded positively to increased agricultural inputs, especially nitrogen. Optimal soil pH and medium phosphorus levels were identified as crucial for maximizing yield. The findings underscore the importance of tailored nutrient management and adaptive strategies to mitigate the adverse effects of climate variability on cereal production. The results provide insights for field crop research and practical approaches to sustain cereal production in changing climatic conditions. Full article
(This article belongs to the Section Agroecology Innovation: Achieving System Resilience)
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<p>Locations of the cereal experiments that took place between 2009 and 2022 in Poland.</p>
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<p>CWB (Climatic Water Balance in mm)—CWB1 (21.03–20.05) for 2020.</p>
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<p>Regression analysis for mean CWB (Climatic Water Balance in mm) across all locations presenting changes over the study period (2009–2022). The orange line represents the trend for CWB4 and the blue one for CWB7; R<sup>2</sup> is the coefficient of determination, and both p-values suggest a statistically significant downward trend (at a significance level of 0.05).</p>
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<p>CART analysis based on the data for 2009–2022 for the grain yield prediction of the studied cereals.</p>
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<p>CART analysis based on the data for 2009–2022 for the grain yield prediction of the studied cereals.</p>
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28 pages, 1166 KiB  
Review
Processing Tomato and Potato Response to Biostimulant Application in Open Field: An Overview
by Marco Francesco Golin, Vittoria Giannini, Marco Bagarello, Wendy Carolina Vernaza Cartagena, Maria Giordano and Carmelo Maucieri
Agronomy 2024, 14(11), 2699; https://doi.org/10.3390/agronomy14112699 - 16 Nov 2024
Viewed by 159
Abstract
Biostimulants include a wide array of microorganisms and substances that can exert beneficial effects on plant development and growth, often enhancing nutrient uptake and improving tolerance against abiotic and biotic stress. Depending on their composition and time of application, these products can influence [...] Read more.
Biostimulants include a wide array of microorganisms and substances that can exert beneficial effects on plant development and growth, often enhancing nutrient uptake and improving tolerance against abiotic and biotic stress. Depending on their composition and time of application, these products can influence plant physiology directly as growth regulators or indirectly through environmental condition changes in the rhizosphere, such as nutrient and water availability. This review evaluated 48 case studies from 39 papers to summarize the effects of biostimulant application on fruit and tuber yields and on the quality of processing tomato and potato in open field conditions. For potato, PGPR bacteria were the main studied biostimulant, whereas the low number of studies on processing tomato did not permit us to delineate a trend. The yield and quality were greatly influenced by cultivars and biostimulant composition, application method, period, and dose. For processing tomato, a positive effect of the biostimulant application on the marketable yield was reported in 79% of the case studies, whereas for potato, the effect was reported in only 47%. Few studies, on processing tomato and potato, also reported data for quality parameters with contrasting results. The variability of crop response to biostimulant application in open field conditions highlights the need for more comprehensive studies. Such studies should focus on diverse cultivars, deeply understand the interaction of biostimulant application with agronomic management (e.g., irrigation and fertilization), and evaluate yield and quality parameters. This approach is crucial to fully understand the potential and limitations of biostimulant applications in agriculture, particularly regarding their role in sustainable crop production. Full article
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<p>Research scheme.</p>
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<p>Locations of processing tomato and potato field trials considered for this review.</p>
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11 pages, 3326 KiB  
Article
One-Step Multiplex RT-PCR Method for Detection of Melon Viruses
by Sheng Han, Tingting Zhou, Fengqin Zhang, Jing Feng, Chenggui Han and Yushanjiang Maimaiti
Microorganisms 2024, 12(11), 2337; https://doi.org/10.3390/microorganisms12112337 - 15 Nov 2024
Viewed by 349
Abstract
This study presents a one-step multiplex reverse transcription polymerase chain reaction (RT-PCR) method for the simultaneous detection of multiple viruses affecting melon crops. Viruses such as Watermelon mosaic virus (WMV), Cucumber mosaic virus (CMV), Zucchini yellow mosaic virus (ZYMV), Squash mosaic virus (SqMV), [...] Read more.
This study presents a one-step multiplex reverse transcription polymerase chain reaction (RT-PCR) method for the simultaneous detection of multiple viruses affecting melon crops. Viruses such as Watermelon mosaic virus (WMV), Cucumber mosaic virus (CMV), Zucchini yellow mosaic virus (ZYMV), Squash mosaic virus (SqMV), Tobacco mosaic virus (TMV), Papaya ring spot virus (PRSV), and Melon yellow spot virus (MYSV) pose a great threat to melons. The mixed infection of these viruses is the most common observation in the melon-growing fields. In this study, we surveyed northern Xingjiang (Altay, Changji, Wujiaqu, Urumqi, Turpan, and Hami) and southern Xingjiang (Aksu, Bayingolin, Kashgar, and Hotan) locations in Xinjiang province and developed a one-step multiplex RT-PCR to detect these melon viruses. The detection limits of this multiplex PCR were 103 copies/μL for ZYMV and MYSV and 102 copies/μL for WMV, SqMV, PRSV, CMV, and TMV. The detection results in the field showed 242 samples were infected by one or more viruses. The multiplex RT-PCR protocol demonstrated rapid, simultaneous, and relatively effective detection of viruses such as WMV, CMV, ZYMV, SqMV, TMV, PRSV, and MYSV. The technique is designed to identify these melon viruses in a single reaction, enhancing diagnostic efficiency and reducing costs, thus serving as a reference for muskmelon anti-virus breeding in Xinjiang. Full article
(This article belongs to the Section Virology)
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<p>The map shows the locations of sampling sites across various prefectures in Xinjiang, marked by red pins. Prefectures include Aksu, Altay, Kashi, Hotan, Hami, Turpan, and Bayingolin Mongolian Autonomous Prefecture, with notable cities such as Urumqi and Changji Hui Autonomous Prefecture also highlighted. The boundaries of Xinjiang, China’s international borders, and various administrative regions are outlined, with a smaller inset map showing the location of Xinjiang within China.</p>
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<p>The images show different degrees of chlorosis, mosaic patterns, and leaf deformation in melon plants across treatments. Panels (<b>A1</b>–<b>J3</b>) depict variations in disease symptoms such as yellowing, curling, and blistering. Each row represents a different set of treatments, with individual images (<b>A1</b>–<b>J3</b>) highlighting specific responses of the leaves to potential stressors. These visible symptoms suggest the presence of viral or environmental stress, with severity and patterns differing across the treatments.</p>
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<p>(<b>A</b>). The specificity analysis results of the multiplex RT-PCR detection method. The detection samples corresponding to lanes 1 to 7 only contain WMV, CMV, ZYMV, SqMV, TMV, PRSV, and MYSV, respectively. (<b>B</b>). The sensitivity tests of the multiplex RT-PCR detection method. The drop-out experiments were carried out to test the specificity of this multiplex RT-PCR, in which one pair was removed at a time to see whether the rest of the primers had cross-reacted.; lane 1: healthy plant (negative control); lane 2: detection of MYSV; lane 3: detection of PRSV; lane 4: detection of TMV; lane 5: detection of SqMV; lane 6: detection of ZYMV; lane 7: detection of CMV. (<b>C1</b>). Detection results of using different Mg<sup>2+</sup> concentrations in multiplex RT-PCR amplification system. lane 1: at 1.0 mol/L; lane 2: at 1.5 mmol/L; lane 3: at 2.0 mmol/L; lane 4: at 2.5 mmol/L; lane 5: at 3.0 mmol/L; lane 6: at 3.5 mmol/L; lane 7: at 4.0 mmol/L. (<b>C2</b>). Detection results of using different template cDNA volumes in multiplex RT-PCR amplification system. lane 1: at 0.5 μL; lane 2: at 0.75 μL; lane 3: at 1 μL; lane 4: at 1.25 μL; lane 5: at 1.5 μL; lane 6: at 1.75 μL; lane 7: at 2 μL. (<b>C3</b>). Detection results of using different primer amounts in a multiplex RT-PCR amplification system; lane 1: at 2 × 10<sup>−5</sup> μmol, and molar ratios of primers for WMV, CMV, ZYMV, SqMV, TMV, PRSV, and MYSV is 1:1:1:1:1:1:1; lane 2: at 2 × 10<sup>−5</sup> μmol, and molar ratios of primers for WMV, CMV, ZYMV, SqMV, TMV, PRSV, and MYSV is 2:2:2:1:1:1:1; lane 3: at 2 × 10<sup>−5</sup> μmol, and molar ratios of primers for WMV, CMV, ZYMV, SqMV, TMV, PRSV, and MYSV is 10:9:8:8:5:5:3:3; lane 4: at 5 × 10<sup>−5</sup> μmol, and molar ratios of primers for WMV, CMV, ZYMV, SqMV, TMV, PRSV, and MYSV is 1:1:1:1:1:1:1; lane 5: at 5 × 10<sup>−5</sup> μmol, and molar ratios of primers for WMV, CMV, ZYMV, SqMV, TMV, PRSV, and MYSV is 2:2:2:1:1:1:1; lane 6: at 5 × 10<sup>−5</sup> μmol, and molar ratios of primers for WMV, CMV, ZYMV, SqMV, TMV, PRSV, and MYSV is 10:9:8:8:5:5:3:3; lane 7: at 7 × 10<sup>−5</sup> μmol, and molar ratios of primers for WMV, CMV, ZYMV, SqMV, TMV, PRSV, and MYSV is 1:1:1:1:1:1:1. (<b>C4</b>). Detection results of using different dNTP concentrations in multiplex RT-PCR amplification system; lane 1: at 0.2 mmol/L; lane 2: at 0.4 mmol/L; lane 3: at 0.6 mmol/L; lane 4: at 0.8 mmol/L; lane 5: at 1.0 mmol/L; lane 6: at 1.2 mmol/L; lane 7: at 1.4 mmol/L; (<b>C5</b>). Detection results of different amounts of Taq DNA polymerase in multiplex RT-PCR amplification system; lane 1: at 0.25 U; lane 2: at 0.5 U; lane 3: at 0.75 U; lane 4: at 1.0 U; lane 5: at 1.25 U; lane 6: at 1.5 U; lane 7: at 1.75 U. (<b>C6</b>). Detection results of using different annealing temperatures in multiplex RT-PCR method; lane 1: at 50 °C; lane 2: at 51 °C; lane 3: at 52 °C; lane 4: at 53 °C; lane 5: at 54 °C; lane 6: at 55 °C; lane 7: at 56 °C. (<b>D</b>). The detection limits of the multiplex RT-PCR assays. The detection limits of this multiplex PCR were conducted by a series of sensitivity tests. The positive clone vector was adjusted to the same initial concentration and diluted serially ten-fold (10<sup>5</sup> to 10<sup>10</sup> copies/μL) to serve as a template in the optimized multiplex PCR. (<b>D1</b>). The detection limits of WMV; 1–6 stand for 10<sup>5</sup> to 10<sup>0</sup> copies/μL. (<b>D2</b>). The detection limits of CMV;1–6 stand for 10<sup>5</sup> to 10<sup>0</sup> copies/μL. (<b>D3</b>). The detection limits of ZYMV; 1–6 stand for 10<sup>5</sup> to 10<sup>0</sup> copies/μL (<b>D4</b>). The detection limits of SqMV; 1–6 stand for 10<sup>5</sup> to 10<sup>0</sup> copies/μL. (<b>D5</b>). The detection limits of TMV; 1–6 stand for 10<sup>5</sup> to 10<sup>0</sup> copies/μL. (<b>D6</b>). The detection limits of PRSV; 1–6 stand for 10<sup>5</sup> to 10<sup>0</sup> copies/μL. (<b>D7</b>). The detection limits of MYSV; 1–6 stand for 10<sup>5</sup> to 10<sup>0</sup> copies/μL. ”–“represents deionized water as control. M: DNA marker (100 bp–2000 bp).</p>
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<p>(<b>A</b>). The heatmap displays the distribution and frequency of various viral diseases affecting melon crops across different regions in northern and southern Xinjiang. The regions include Altay, Changji, Wujiaqu, Urumqi, Turpan, Hami, Aksu, Bayingolin, Kashgar, and Hotan. Viral diseases represented are <span class="html-italic">Watermelon Mosaic Virus</span> (WMV), <span class="html-italic">Cucumber Mosaic Virus</span> (CMV), <span class="html-italic">Zucchini Yellow Mosaic Virus</span> (ZYMV), <span class="html-italic">Squash Mosaic Virus</span> (SqMV), <span class="html-italic">Tobacco Mosaic Virus</span> (TMV), <span class="html-italic">Papaya Ringspot Virus</span> (PRSV), and <span class="html-italic">Melon Yellow Spot Virus</span> (MYSV). The color intensity corresponds to the number of cases, with red indicating higher incidence. The total number of cases (N = 242) per virus and per region is shown, with regions in southern Xinjiang showing a generally higher disease incidence compared to northern Xinjiang. (<b>B</b>). This figure shows the results of differential analysis of detection rates among different viruses in the Xinjiang region. The data of each virus detection rate on the horizontal axis is the average of the virus detection rates in 10 locations in Xinjiang. This figure shows the analysis results of the differences in virus detection rates between different regions. The horizontal axis represents different virus types, and the vertical axis represents virus detection rates. (<b>C</b>). Detection rate of <span class="html-italic">Watermelon Mosaic Virus</span> (WMV), <span class="html-italic">Cucumber Mosaic Virus</span> (CMV), <span class="html-italic">Zucchini Yellow Mosaic Virus</span> (ZYMV), <span class="html-italic">Squash Mosaic Virus</span> (SqMV), <span class="html-italic">Tobacco Mosaic Virus</span> (TMV), <span class="html-italic">Papaya Ringspot Virus</span> (PRSV), and <span class="html-italic">Melon Yellow Spot Virus</span> (MYSV) at different locations of Xinjiang. The reaction experimental results of lowercase letters reach the 0.05 significance level. Different letters represent significant differences between groups, and the same letters or shared letters represent insignificant differences between groups.</p>
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<p>Results of multiplex RT-PCR detection method for virus disease samples from different regions; M: Marker; 1–24: Samples of melon plants infected by virus diseases in different regions of Xinjiang.</p>
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12 pages, 2659 KiB  
Article
CO2 Flux Emissions by Fixed and Mobile Soil Collars Under Different Pasture Management Practices
by Paulo Roberto da Rocha Junior, Felipe Vaz Andrade, Guilherme Kangussú Donagemma, Fabiano de Carvalho Balieiro, Eduardo de Sá Mendonça, Adriel Lima Nascimento, Fábio Ribeiro Pires and André Orlandi Nardotto Júnior
AgriEngineering 2024, 6(4), 4325-4336; https://doi.org/10.3390/agriengineering6040244 (registering DOI) - 15 Nov 2024
Viewed by 218
Abstract
Carbon dioxide flux emissions (CFE) from agricultural areas exhibit spatial and temporal variability, and the best time of collar fixation to the soil prior to the collection of CO2 flux, or even its existence as a factor, is unclear. The objective of [...] Read more.
Carbon dioxide flux emissions (CFE) from agricultural areas exhibit spatial and temporal variability, and the best time of collar fixation to the soil prior to the collection of CO2 flux, or even its existence as a factor, is unclear. The objective of this study was to evaluate the effect of the fixation time of collars that support the soil-gas flux chamber based on the influence of CFE on different pasture management practices: control (traditional pasture management practice) (CON), chisel (CHI), fertilized (FER), burned (BUR), integrated crop-livestock (iCL), and plowing and harrowing (PH). A field study was conducted on the clayey soil of Udults. The evaluations were performed monthly by fixing the PVC collars 30 d and 30 min prior to each CFE measurement. Although a linear trend in CFE was observed within each pasture management practice between the two collar-fixation times, collar fixation performed 30 min prior led to an overestimation of CFE by approximately 32.7% compared with 30 d of collar fixation. Thus, CFE were higher (p ≤ 0.10) in the MC, when compared to the FC, when the CON, BUR, and iCL managements were evaluated. Overall, fixing the collar 30 d prior to field data collection can improve the quality of the data, making the results more representative of actual field conditions. Full article
(This article belongs to the Section Livestock Farming Technology)
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Figure 1
<p>Experimental location and the different pasture management practices studied.</p>
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<p>Mean ± standard deviation of CO<sub>2</sub> (μmol m<sup>−2</sup>·s<sup>−1</sup>) flux emissions after fixing the PVC collars 30 d (FC) and 30 min (MC) prior to data collection, with six months of evaluation under different pasture management practices. Within each pasture management practice, the means with the same letter are statistically equal based on the Scott–Knott group of means (<span class="html-italic">p</span> ≤ 0.10).</p>
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<p>Scheme of chambers and mean values of CO<sub>2</sub> (μmol m<sup>−2</sup>·s<sup>−1</sup>) flux emissions after fixing the PVC collars 30 d (FC) and 30 min (MC) prior to data collection.</p>
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<p>Mean ± standard deviation of CO<sub>2</sub> (μmol m<sup>−2</sup>·s<sup>−1</sup>) flux emissions after fixing the PVC collars 30 d (FC) and 30 min (MC) prior to data collection with 6 months of evaluation (1 March/2014; 2 April/2014; 3 May/2014; 4 June/2014; 5 July/2014; 6 August/2014) in different pasture management practices. *** and ** indicate significance at 0.01% and 0.05%, respectively.</p>
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<p>Relationship between CO<sub>2</sub> (μmol m<sup>−2</sup>·s<sup>−1</sup>) flux emissions, temperature (°C), and soil moisture (%) after fixing the PVC collars 30 d (FC) and 30 min (MC) prior to data collection with six months of evaluation (1 March/2014; 2 April/2014; 3 May/2014; 4 June/2014; 5 July/2014; 6 August/2014) in different pasture management practices.</p>
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14 pages, 570 KiB  
Article
Understanding the Impact of Soil Characteristics and Field Management Strategies on the Degradation of a Sprayable, Biodegradable Polymeric Mulch
by Cuyler K. Borrowman, Raju Adhikari, Kei Saito, Stuart Gordon and Antonio F. Patti
Agriculture 2024, 14(11), 2062; https://doi.org/10.3390/agriculture14112062 (registering DOI) - 15 Nov 2024
Viewed by 197
Abstract
The use of non-degradable plastic mulch has become an essential agricultural practice for increasing crop yields, but continued use has led to contamination problems and in some cropping areas decreases in agricultural productivity. The subsequent emergence of biodegradable plastic mulches is a technological [...] Read more.
The use of non-degradable plastic mulch has become an essential agricultural practice for increasing crop yields, but continued use has led to contamination problems and in some cropping areas decreases in agricultural productivity. The subsequent emergence of biodegradable plastic mulches is a technological solution to these issues, so it is important to understand how different soil characteristics and field management strategies will affect the rate at which these new materials degrade in nature. In this work, a series of lab-scale hydrolytic degradation experiments were conducted to determine how different soil characteristics (type, pH, microbial community composition, and particle size) affected the degradation rate of a sprayable polyester–urethane–urea (PEUU) developed as a biodegradable mulch. The laboratory experiments were coupled with long-term, outdoor, soil degradation studies, carried out in Clayton, Victoria, to build a picture of important factors that can control the rate of PEUU degradation. It was found that temperature and acidity were the most important factors, with increasing temperature and decreasing pH leading to faster degradation. Other important factors affecting the rate of degradation were the composition of the soil microbial community, the mass loading of PEUU on soil, and the degree to which the PEUU was in contact with the soil. Full article
(This article belongs to the Section Ecosystem, Environment and Climate Change in Agriculture)
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