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Search Results (32,331)

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16 pages, 275 KiB  
Article
Effect of Usage of Industrial Robots on Quality, Labor Productivity, Exports and Environment
by Iztok Palčič and Jasna Prester
Sustainability 2024, 16(18), 8098; https://doi.org/10.3390/su16188098 (registering DOI) - 16 Sep 2024
Abstract
Industrial robots are slowly finding their way into manufacturing companies. This paper examines the impact of robots on productivity, exports, quality, sustainability and labor in European manufacturing companies. There is little research on the use of industrial robots and their impact in developed [...] Read more.
Industrial robots are slowly finding their way into manufacturing companies. This paper examines the impact of robots on productivity, exports, quality, sustainability and labor in European manufacturing companies. There is little research on the use of industrial robots and their impact in developed countries. Most research relates to Chinese companies, and often, the data are outdated. The data in this paper come from the European Manufacturing Survey project, which was conducted in 2022 and includes 476 manufacturing companies. The results of the impact of industrial robots on quality, labor productivity, exports and green technologies are determined using a T-test between companies that use industrial robots and those that do not. However, the impact of higher investment in environmental technologies by industrial robot users was examined by a two-stage OLS regression analysis with control variables representing the contextual characteristics of the companies. The results show positive effects on all of the variables. The results show that the greater use of robots occurs in industries with low-to-medium technology intensity, that robots contribute to labor productivity and exports and that companies that use robots also tend to use environmentally friendly technologies. Full article
(This article belongs to the Section Sustainable Management)
21 pages, 13066 KiB  
Article
The Influence of Morphological Elements of Urban Gated Communities on Road Network Connectivity: A Study of 120 Samples of the Central Districts of Jinan, China
by Xinxin Hao, Jilong Zhao, Qingtan Deng, Siyu Wang, Canyi Che and Yuxiang Chen
Sustainability 2024, 16(18), 8095; https://doi.org/10.3390/su16188095 (registering DOI) - 16 Sep 2024
Abstract
Currently, the dominant gated communities (GCs) in Chinese cities have fragmented the urban road network, causing traffic congestion, energy consumption, carbon emissions, and environmental pollution. The morphological elements of GCs are key factors affecting road network connectivity. This paper aimed to explore the [...] Read more.
Currently, the dominant gated communities (GCs) in Chinese cities have fragmented the urban road network, causing traffic congestion, energy consumption, carbon emissions, and environmental pollution. The morphological elements of GCs are key factors affecting road network connectivity. This paper aimed to explore the influence of the morphological elements of GCs on road network connectivity, to provide a quantitative basis for the evaluation and renovation of the connectivity of GCs, and to provide insights for urban planning and policy. This paper quantitatively analyzed the connectivity of GCs using 120 samples from the central districts of Jinan, China. Morphological elements were the independent variables, while route directness (RD) and the network distance (D) to the nearest entrance were the dependent variables. RD measured the internal connectivity, and D measured the connectivity between the internal and external road networks of GCs. GIS was used to measure RD and D, and SPSS was used to conduct a correlation analysis to identify significant variables. Multiple linear regression and LASSO regression were used to test the influence of these factors on RD and D. LASSO regression was employed to construct prediction models for RD and D. We found that intersection density had the greatest impact on RD, while the number of entrances and exits, and the scale of GCs, had the greatest impact on D. Using thresholds of D = 250 and RD = 1.3, the four types of GCs were classified and corresponding renovation measures were proposed. Full article
(This article belongs to the Collection Urban Street Networks and Sustainable Transportation)
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<p>The 120 gated communities in central districts of Jinan.</p>
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<p>Intersection form of the road network in a gated community.</p>
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<p>(<b>a</b>) Route directness; (<b>b</b>) shortest network distance to the nearest entrance.</p>
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<p>Flowchart of this work.</p>
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<p>Analysis of correlations between morphological elements and RD based on Pearson’s correlation coefficient.</p>
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<p>Analysis of correlations between morphological elements and D based on Pearson’s correlation coefficient.</p>
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<p>The cross-validation for the Lasso model of RD. (<b>a</b>) MSE dependence on lambda. (<b>b</b>) Coefficient path dependence on lambda.</p>
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<p>The cross-validation for the Lasso model of D. (<b>a</b>) MSE dependence on lambda. (<b>b</b>) Coefficient paths dependence on lambda.</p>
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19 pages, 5307 KiB  
Article
Energy Monitoring and Analysis of a Residential House in China
by Yanzhi Wang, Shaotong Han, Qiuqi Zhang, Jing Sun, Zhibao Cheng and An Chen
Buildings 2024, 14(9), 2930; https://doi.org/10.3390/buildings14092930 (registering DOI) - 16 Sep 2024
Abstract
The energy consumption of residential buildings plays a crucial role in overall energy consumption and environmental sustainability. This paper aims to conduct an energy analysis of a residential house located in China, with a focus on comparing the accuracy of the model, identifying [...] Read more.
The energy consumption of residential buildings plays a crucial role in overall energy consumption and environmental sustainability. This paper aims to conduct an energy analysis of a residential house located in China, with a focus on comparing the accuracy of the model, identifying areas for improvement, and proposing energy-efficient solutions. Four sets of temperature sensors were placed to monitor the ambient temperature at which the building is located and the indoor temperature of the residential building during a heating season. The energy consumption of keeping the building running at a low temperature was recorded and compared with the simulation results to verify the accuracy of the model. The monitoring results give the weekly average temperature of each zone on each floor, and the door and window positions, room layouts, and orientations are discussed to analyze the thermal response of the building. In addition, the effect of the heat transfer coefficient of the exterior walls, the heat transfer coefficient of the roof, and the solar heat gain coefficient (SHGC) of the exterior windows on the heating energy consumption of the building are further analyzed through simulations. The results show that, after adding a certain thickness of insulation to the exterior walls and roofs of a building, increasing the thickness of the insulation layer produces little extra energy saving. The use of building windows with high SHGC can effectively reduce building heating energy consumption. Full article
(This article belongs to the Special Issue Building Energy-Saving Technology—2nd Edition)
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<p>Building photo.</p>
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<p>Geographical location of the house and surrounding buildings.</p>
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<p>Wallboard configuration.</p>
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<p>Roof configuration.</p>
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<p>Floor configuration.</p>
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<p>Locations of indoor temperature sensors (unit: mm): red color denotes sensors’ locations, where the pentagram and circle indicate locations of the main unit and temperature sensor, respectively.</p>
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<p>Locations of outdoor temperature sensors (unit: mm): red color denotes sensors’ locations, where the pentagram and circle indicate locations of the main unit and temperature sensor, respectively.</p>
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<p>Building model (balconies not included).</p>
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<p>Modelling flowchart: blue dashed line represents settings and functions of each software.</p>
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<p>Outdoor temperature monitoring data.</p>
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<p>Weekly average indoor temperatures for each zone on each floor of the building.</p>
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<p>Influence of the thermal resistance of exterior walls on building heating energy consumption.</p>
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<p>Influence of heat transfer coefficients of exterior walls on building heating energy consumption.</p>
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<p>Influence of roof thermal resistance on building heating energy consumption.</p>
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<p>Influence of roof heat transfer coefficient on building heating energy consumption.</p>
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<p>Influence of exterior window solar heat gain coefficients on building heating energy consumption.</p>
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22 pages, 2278 KiB  
Review
Perspectives on the Toxic Effects of Micro- and Nanoplastics on the Environment: A Bibliometric Analysis of the 2014 to 2023 Period
by Xianhong Li and Zhonghong Li
Toxics 2024, 12(9), 676; https://doi.org/10.3390/toxics12090676 (registering DOI) - 16 Sep 2024
Abstract
Over the past decade, micro- and nanoplastics (MNPs) have garnered significant attention due to their frequent detection in and potential toxic effects on the environment and organisms, making them a serious threat to human health. To comprehensively understand the research on MNPs’ toxicity, [...] Read more.
Over the past decade, micro- and nanoplastics (MNPs) have garnered significant attention due to their frequent detection in and potential toxic effects on the environment and organisms, making them a serious threat to human health. To comprehensively understand the research on MNPs’ toxicity, we employed the R language-based Bibliometrix toolkit (version 4.3.0), VOSviewer (version 1.6.11) and CiteSpace (version 6.3.R1) to perform statistical and visual analyses of 3541 articles pertaining to MNPs’ toxicity between 2014 and 2023, which were retrieved from the Web of Science Core Collection (WOSCC) database. The analysis revealed that research related to MNPs’ toxicity has experienced a rapid increase in recent years. China’s particularly prominent influence in the field of MNPs’ toxicity is evidenced by its academic exchanges and the establishment of a mature cooperation system with other countries (regions), such as the USA and Germany. Studies related to MNPs’ toxicity are primarily published in leading journals, including the Science of the Total Environment, Environmental Pollution, and the Journal of Hazardous Materials. The Chinese Academy of Sciences was identified as the leading institution in terms of research on MNPs’ toxicity, contributing 203 papers to the total number of studies published. Keyword co-occurrence and burst analyses indicated that the current research on MNPs’ toxicity mainly focuses on the toxic effects of MNPs on aquatic organisms, the combined toxicity of MNPs and other contaminants, and the toxic effects and mechanisms of MNPs. Future research should integrate computational toxicology and toxicomics to enhance our understanding of MNPs’ toxicity mechanisms and assess the potential health risks posed by atmospheric MNPs. Full article
(This article belongs to the Section Novel Methods in Toxicology Research)
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<p>A flowchart of the bibliometric analysis and methodology used.</p>
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<p>Number of publications and mean citations per article on MNPs’ toxicity during the 2014–2023 period.</p>
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<p>Top 5 subject categories related to research on MNPs’ toxicity, published from 2014 to 2023 in the WOSCC database.</p>
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<p>The top 20 countries/regions in terms of the number of publications generated that relate to MNPs’ toxicity between 2014 and 2023.</p>
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<p>Network visualization of keyword co-occurrence network.</p>
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<p>The top 25 keywords with the strongest citation burst in articles on MNPs’ toxicity published from 2014 to 2023.</p>
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13 pages, 751 KiB  
Review
Research Progress on Photoperiod Gene Regulation of Heading Date in Rice
by Jian Song, Liqun Tang, Yongtao Cui, Honghuan Fan, Xueqiang Zhen and Jianjun Wang
Curr. Issues Mol. Biol. 2024, 46(9), 10299-10311; https://doi.org/10.3390/cimb46090613 (registering DOI) - 16 Sep 2024
Abstract
Heading date is a critical physiological process in rice that is influenced by both genetic and environmental factors. The photoperiodic pathway is a primary regulatory mechanism for rice heading, with key florigen genes Hd3a (Heading date 3a) and RFT1 (RICE FLOWERING LOCUS T1) [...] Read more.
Heading date is a critical physiological process in rice that is influenced by both genetic and environmental factors. The photoperiodic pathway is a primary regulatory mechanism for rice heading, with key florigen genes Hd3a (Heading date 3a) and RFT1 (RICE FLOWERING LOCUS T1) playing central roles. Upstream regulatory pathways, including Hd1 and Ehd1, also significantly impact this process. This review aims to provide a comprehensive examination of the localization, cloning, and functional roles of photoperiodic pathway-related genes in rice, and to explore the interactions among these genes as well as their pleiotropic effects on heading date. We systematically review recent advancements in the identification and functional analysis of genes involved in the photoperiodic pathway. We also discuss the molecular mechanisms underlying rice heading date variation and highlight the intricate interactions between key regulatory genes. Significant progress has been made in understanding the molecular mechanisms of heading date regulation through the cloning and functional analysis of photoperiod-regulating genes. However, the regulation of heading date remains complex, and many underlying mechanisms are not yet fully elucidated. This review consolidates current knowledge on the photoperiodic regulation of heading date in rice, emphasizing novel findings and gaps in the research. It highlights the need for further exploration of the interactions among flowering-related genes and their response to environmental signals. Despite advances, the full regulatory network of heading date remains unclear. Further research is needed to elucidate the intricate gene interactions, transcriptional and post-transcriptional regulatory mechanisms, and the role of epigenetic factors such as histone methylation in flowering time regulation. This review provides a detailed overview of the current understanding of photoperiodic pathway genes in rice, setting the stage for future research to address existing gaps and improve our knowledge of rice flowering regulation. Full article
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<p>Photoperiodic regulatory pathway of rice flowering. (<b>a</b>) Under short-day conditions, <span class="html-italic">OsGI</span> activates <span class="html-italic">Hd1</span> and <span class="html-italic">Ehd1</span>, which promote the expression of the florigen gene <span class="html-italic">Hd3a</span>, triggering the transition from the vegetative to the reproductive phase. Various genes, such as <span class="html-italic">Ghd8</span>, <span class="html-italic">OsCOL4</span>, and <span class="html-italic">MADS51</span>, regulate <span class="html-italic">Ehd1</span> and <span class="html-italic">Hd1</span>, either enhancing or repressing their effects. (<b>b</b>) Under long-day conditions, <span class="html-italic">OsGI</span> activates <span class="html-italic">Hd1</span>, which represses <span class="html-italic">Hd3a</span>, while <span class="html-italic">Ehd1</span> activates <span class="html-italic">Hd3a/RFT1</span> to promote flowering. Other genes, including <span class="html-italic">Ghd7, DTH7</span>, and <span class="html-italic">OsTrx1</span>, modulate this pathway by controlling <span class="html-italic">Hd1</span> and <span class="html-italic">Ehd1</span> activity.</p>
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22 pages, 7490 KiB  
Article
Incorporating Ecosystem Service Trade-Offs and Synergies with Ecological Sensitivity to Delineate Ecological Functional Zones: A Case Study in the Sichuan-Yunnan Ecological Buffer Area, China
by Peipei Miao, Cansong Li, Baichuan Xia, Xiaoqing Zhao, Yingmei Wu, Chao Zhang, Junen Wu, Feng Cheng, Junwei Pu, Pei Huang, Xiongfei Zhang and Yi Chai
Land 2024, 13(9), 1503; https://doi.org/10.3390/land13091503 (registering DOI) - 16 Sep 2024
Abstract
Enhancing regional ecosystem stability and managing land resources effectively requires identifying ecological function zones and understanding the factors that influence them. However, most current studies have primarily focused on ecosystem service bundles, paying less attention to the trade-offs, synergies, and ecological sensitivity, leading [...] Read more.
Enhancing regional ecosystem stability and managing land resources effectively requires identifying ecological function zones and understanding the factors that influence them. However, most current studies have primarily focused on ecosystem service bundles, paying less attention to the trade-offs, synergies, and ecological sensitivity, leading to a more uniform approach to functional zoning. This study aimed to analyze and describe the spatial and temporal patterns of four essential ecosystem services, including water yield (WY), net primary productivity (NPP), soil conservation (SC), and habitat quality (HQ), in the Sichuan-Yunnan ecological buffer area over the period from 2005 to 2019. Spatial overlay analysis was used to assess ecological sensitivity, trade-offs, synergies, and ecosystem service bundles to define ecological functional zones. Geographic detectors were then applied to identify the primary drivers of spatial variation in these zones. The findings showed a progressive improvement in ecosystem service functions within the Sichuan-Yunnan ecological buffer zone. Between 2005 and 2019, NPP, soil conservation, and water yield all demonstrated positive trends, while HQ displayed a declining trend. There was significant spatial heterogeneity and distinct regional patterns in ecosystem service functions, with a general decrease from southwest to northeast, particularly in NPP and HQ. Trade-offs were evident in most ecosystem services, with the most significant between WY and HQ and most in the northeast and east regions. Ecological sensitivity decreased from southwest to northeast. Regions with a higher ecological sensitivity were primarily situated in the southwestern region, and their spatial distribution pattern was comparable to that of high habitat quality. The spatial overlay analysis categorized areas into various types, including human production and settlement zones, ecologically vulnerable zones, ecological transition zones, and ecological conservation zones, accounting for 17.28%, 22.30%, 7.41%, and 53.01% of the total area, respectively. The primary environmental factor affecting ecological function zoning was identified as precipitation, while the main social variables were human activity and population density. This study enhances the understanding of ecological functions and supports sustainable development in the Sichuan-Yunnan ecological buffer area, offering important guidance for ecological zoning. Full article
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<p>Study area.</p>
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<p>Land cover type.</p>
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<p>Research framework. (AHP: the analytical hierarchy process; R: R language; GDP: gross domestic product).</p>
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<p>The annual average value of four ecosystem services in the ecological conservation area of Sichuan-Yunnan provinces from 2005 to 2019. (HQ: habitat quality).</p>
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<p>Ecosystem service proportion trends over time. (HQ: habitat quality).</p>
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<p>Spatio-temporal distribution and variations of ecosystem services in the ecological conservation area of Sichuan-Yunnan provinces from 2000 to 2019. Note: (<b>a</b>) NPP (net primary productivity), (<b>b</b>) WY (water yield), (<b>c</b>) HQ (habitat quality), (<b>d</b>) SC (soil conservation).</p>
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<p>Trade-offs/synergy analysis of temporal changes in ecosystem services.</p>
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<p>Trade-offs/synergy analysis of spatial changes in ecosystem services. (HQ: habitat quality).</p>
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<p>Comprehensive spatial pattern of ecological sensitivity.</p>
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<p>Classification results of ecosystem service bundles (<b>a</b>) and radar chart of ecosystem services by service cluster (<b>b</b>).</p>
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<p>Spatial distribution and pattern of ecosystem service clusters in the ecological conservation area in Sichuan-Yunnan provinces from 2005 to 2019 (ESB: Ecosystem service bundles).</p>
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<p>Ecosystem service function divisions in ecological conservation areas in Sichuan-Yunnan provinces.</p>
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<p>Results of the interaction between factors in the study area. [X1–X8 for each of the eight impact factors: land use type, normalized difference vegetation index (NDVI), nighttime lighting, population density, human activities, temperature, precipitation, and topographic relief].</p>
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<p>Rainfall and temperature changes in Sichuan-Yunnan provinces from 2005 to 2019.</p>
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23 pages, 5477 KiB  
Article
Transcriptome Analysis Identified PyNAC42 as a Positive Regulator of Anthocyanin Biosynthesis Induced by Nitrogen Deficiency in Pear (Pyrus spp.)
by Jianhui Zhang, Bobo Song, Guosong Chen, Guangyan Yang, Meiling Ming, Shiqiang Zhang, Zhaolong Xue, Chenhui Han, Jiaming Li and Jun Wu
Horticulturae 2024, 10(9), 980; https://doi.org/10.3390/horticulturae10090980 (registering DOI) - 16 Sep 2024
Abstract
Anthocyanins are important secondary metabolites in plants, which contribute to fruit color and nutritional value. Anthocyanins can be regulated by environmental factors such as light, low temperature, water conditions, and nutrition limitations. Nitrogen (N) is an essential macroelement for plant development, its deficiency [...] Read more.
Anthocyanins are important secondary metabolites in plants, which contribute to fruit color and nutritional value. Anthocyanins can be regulated by environmental factors such as light, low temperature, water conditions, and nutrition limitations. Nitrogen (N) is an essential macroelement for plant development, its deficiency as a kind of nutrition limitation often induces anthocyanin accumulation in many plants. However, there is a lack of reports regarding the effect of nitrogen deficiency on anthocyanin biosynthesis in pears. In this study, we found that N deficiency resulted in anthocyanin accumulation in pear callus and upregulated the expression of anthocyanin biosynthesis pathway structural genes (PyPAL, PyCHS, PyCHI, PyF3H, PyDFR, PyANS, and PyUFGT) and key regulatory factors (PyMYB10, PyMYB114, and PybHLH3). Through analysis of transcriptome data of treated pear callus and RT-qPCR assay, a differentially expressed gene PyNAC42 was identified as significantly induced by the N deficiency condition. Overexpression of PyNAC42 promoted anthocyanin accumulation in “Zaosu” pear peels. Additionally, dual luciferase assay and yeast one-hybrid assay demonstrated that PyNAC42 could not directly activate the expression of PyDFR, PyANS, and PyUFGT. Furthermore, yeast two-hybrid and pull-down assays confirmed that PyNAC42 interacted with PyMYB10 both in vivo and in vitro. Co-expression of PyNAC42 and PyMYB10 significantly enhanced anthocyanin accumulation in “Zaosu” pear peels. Dual luciferase assay showed that PyNAC42 significantly enhanced the activation of PyDFR, PyANS, and PyUFGT promoters by interacting with PyMYB10, which suggests that PyNAC42 can form the PyNAC42-PyMYB10 complex to regulate anthocyanin biosynthesis in pear. Thus, the molecular mechanism underlying anthocyanin biosynthesis induced by N deficiency is preliminarily elucidated. Our finding has expanded the regulatory network of anthocyanin biosynthesis and enhanced our understanding of the mechanisms underlying nutrient deficiency modulates anthocyanin biosynthesis in pear. Full article
(This article belongs to the Section Genetics, Genomics, Breeding, and Biotechnology (G2B2))
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<p>(<b>A</b>) Effects of the MS control (MS) and N-deficiency treatment (-N) on anthocyanin accumulation in pear callus. (<b>B</b>) Total anthocyanin contents of pear callus subjected to two treatments. The <span class="html-italic">X</span>-axis represents (MS) and (-N) treatments. (<b>C</b>) Expression levels of anthocyanin biosynthesis-related genes in pear callus. The data were normalized to the <span class="html-italic">PyGAPDH</span> expression level. The error bars in (<b>B</b>,<b>C</b>) represent the SD (<span class="html-italic">n</span> = 3 independent biological replicates). Student’s <span class="html-italic">t</span>-test was used for statistical analysis (*** <span class="html-italic">p</span> &lt; 0.001; ** <span class="html-italic">p</span> &lt; 0.01.</p>
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<p>Analysis of RNA-seq data. (<b>A</b>) Summary of RNA-sequencing data. (<b>B</b>) The volcano plot showed the DEGs in N-deficiency treatment (-N) vs. MS control (MS) comparison. The up- and downregulated genes were represented by red and blue dots, respectively. The dashed lines represent thresholds for differentially expressed genes, with vertical dashed lines indicating |log2 Fold Change| = 1 and the horizontal dashed line indicating -log10 (FDR) = 2.</p>
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<p>GO enrichment and KEGG pathway analyses of DEGs from N-deficiency treatment vs. MS control comparison. Bubble diagram showing enriched GO (<b>A</b>) biological process, (<b>B</b>) cellular component, and (<b>C</b>) molecular function terms. (<b>D</b>) Bubble diagram showing enriched KEGG pathways terms. The color represents the significance. The red color corresponds to a higher significance, while the blue color corresponds to a lower significance. The important enriched terms were marked in red.</p>
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<p>RT-qPCR validation of nine candidate DEGs from RNA-Seq data analysis. (<b>A</b>–<b>I</b>) The relative expression of nine candidate DEGs in MS control (MS) and N-deficiency treatment (-N). The columns represent the relative expression levels from RT-qPCR (left y axis) The lines represent FPKM values from the RNA-seq data (right y axis). The Pearson correlation coefficient (r) values between FPKM and the relative expression levels are shown. The error bars mean the SD (<span class="html-italic">n</span> = 3). Student’s <span class="html-italic">t</span>-test was used for statistical analysis (*** <span class="html-italic">p</span> &lt; 0.001; ** <span class="html-italic">p</span> &lt; 0.01).</p>
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<p>(<b>A</b>) Phylogenetic relationship between PyNAC42 (marked with a red frame) and other NAC TF from different species (for a complete list of species, see <a href="#app1-horticulturae-10-00980" class="html-app">Table S2</a>). (<b>B</b>) Amino acid sequence alignment of NAC TF PyNAC42 (marked with a red frame), AtNAC42, MdNAC2, AtNAC78, PbrNSC, and PpBL. (*) represents the omitted number of amino acids. The shading color indicates the level of conservation in the amino acid sequence, which is categorized into three levels denoted by black, pink, and blue respectively based on their degree of conservatism. (<b>C</b>) Detection of fluorescence signals in tobacco leaf transformed with PyNAC42-GFP. In the detection, mCherry was used to show the nuclear localization. Bars represent 20 μm.</p>
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<p>(<b>A</b>–<b>D</b>) Functional analysis of the role of PyNAC42 in anthocyanin biosynthesis in “Zaosu” pear peels. (<b>A</b>) Phenotypes of “Zaosu” pear peels transiently transformed with the empty vector (EV) and a vector overexpressing PyNAC42 (<span class="html-italic">PyNAC42</span>-OE). The injection areas were outlined in dash circles. (<b>B</b>) Anthocyanin contents of “Zaosu” pear peels. The <span class="html-italic">X</span>-axis represents two treatments. Expression levels of (<b>C</b>) <span class="html-italic">PyNAC42</span> and (<b>D</b>) anthocyanin biosynthesis structural genes in pear peels. The data were normalized to the <span class="html-italic">PyGAPDH</span> expression level. (<b>E</b>,<b>F</b>) Effect of PyNAC42 on the promoter activity of anthocyanin biosynthesis structural genes. (<b>E</b>) Vector construction for effector and reporters used in dual luciferase assay. (<b>F</b>) Activation effect of PyNAC42 on promoters of <span class="html-italic">PyDFR</span>, <span class="html-italic">PyANS</span>, and <span class="html-italic">PyUFGT</span>. The error bars are SD (<span class="html-italic">n</span> = 3). Student’s <span class="html-italic">t</span>-test was used for statistical analysis (*** <span class="html-italic">p</span> &lt; 0.001; ** <span class="html-italic">p</span> &lt; 0.01).</p>
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<p>(<b>A</b>,<b>B</b>) PyNAC42 interacts with PyMYB10 both in vivo and in vitro. (<b>A</b>) Y2H assay of interactions between PyNAC42 and PyMYB10, PybHLH3, and PyMYB114. Yeast cells were plated on SD/-Leu/-Trp, SD/-Leu/-Trp/-His/-Ade, and SD/-Leu/-Trp/-His/-Ade/+X-α-Gal media. Growth trend aligned with that of the positive control indicates an interaction between the two proteins. (<b>B</b>) Pull-down assay. GST and the PyMYB10-GST fusion protein were bound to GST-Sefinose Resin and incubated with the PyNAC42-HIS protein, respectively. The PyMYB10-GST fusion protein could pull down the PyNAC42-HIS fusion protein, which indicated that PyNAC42 interacts with PyMYB10 in vitro. (<b>C</b>–<b>F</b>) Functional analysis of the effects of the interaction between PyNAC42 and PyMYB10 on anthocyanin biosynthesis in “Zaosu” pear peels. (<b>C</b>) Phenotypes, (<b>D</b>) total anthocyanin contents, and (<b>E</b>,<b>F</b>) expression levels of <span class="html-italic">PyNAC42</span>, <span class="html-italic">PyMYB10</span>, <span class="html-italic">PyDFR</span>, <span class="html-italic">PyANS</span>, and <span class="html-italic">PyUFGT</span> in “Zaosu” pear peels. The <span class="html-italic">X</span>-axis represents four treatments. <span class="html-italic">PyGAPDH</span> was used as reference gene for RT-qPCR, and one-way ANOVA with Tukey’s post hoc test was used for statistical analysis. Different letters indicate a significant difference between means.</p>
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<p>Effects of PyNAC42-PyMYB10 complex on the promoter activation of LBGs. (<b>A</b>) Vector construction for effectors and reporters used in dual luciferase assays. (<b>B</b>) Activation of the promoters of <span class="html-italic">PyDFR</span>, <span class="html-italic">PyANS</span>, and <span class="html-italic">PyUFGT</span> by four treatments. <span class="html-italic">X</span>-axis represents the group of four treatments. Specifically, EV was added to balance the concentration when PyNAC42/PyMYB10 was co-injected with each promoter. The error bars are SD (<span class="html-italic">n</span> = 3). One-way ANOVA with Tukey’s post hoc test was used for statistical analysis, and different letters indicate <span class="html-italic">p</span> &lt; 0.05. The LUC/REN ratio represents the promoter activity.</p>
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<p>Proposed model for underlying N deficiency induced anthocyanin biosynthesis mediated by PyNAC42.</p>
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14 pages, 1968 KiB  
Article
Rice Husk as Raw Material in Synthesis of NaA (LTA) Zeolite
by Daniela Novembre, Domingo Gimeno, Lucia Marinangeli, Anna Chiara Tangari, Gianluigi Rosatelli, Michele Ciulla and Pietro di Profio
Molecules 2024, 29(18), 4396; https://doi.org/10.3390/molecules29184396 (registering DOI) - 16 Sep 2024
Abstract
The present work deals with the hydrothermal synthesis of a Na-A (LTA) zeolite using rice husk as a starting material. The focus was on defining the most favorable conditions for the synthesis of zeolite Na-A from rice husk in order to economize on [...] Read more.
The present work deals with the hydrothermal synthesis of a Na-A (LTA) zeolite using rice husk as a starting material. The focus was on defining the most favorable conditions for the synthesis of zeolite Na-A from rice husk in order to economize on both energy (i.e., synthesis temperatures) and reaction time and to enlarge the field of the pure and isolated synthesized phase. Four sets of experiments were carried out at environmental pressure temperatures varying from 40 °C to 85 °C with a SiO2/Al2O3 ratio from 1.75 to 3.5. Optimal conditions for crystallization of the Na-A zeolite from rice husk were reached at 60 °C with a SiO2/Al2O3 ratio of 1.75. Sixty degrees Celsius represents the minimum known temperature used for the synthesis of NaA zeolite from rice husk. The products of synthesis were characterized by X-ray diffraction, scanning electron microscopy, infrared and Raman spectroscopy. The purity of the synthesized zeolite is verified here for the first time through quantitative phase analysis using the combined Rietveld and reference intensity ratio methods. Full article
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<p>(<b>a</b>) XRPD pattern of RHA. (<b>b</b>) IR analysis on RHA.</p>
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<p>XRPD data. (<b>a</b>) XRPD pattern of Experiment 1, (<b>b</b>) XRPD pattern of Experiment 2.</p>
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<p>XRPD data. (<b>a</b>) XRPD pattern of Experiment 3, (<b>b</b>) XRD pattern of Experiment 4.</p>
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<p>SEM images of NaA zeolite crystals for Experiment 2. (<b>a</b>) 6 h, (<b>b</b>) 8 h.</p>
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<p>SEM images of zeolite crystals for Experiment 3. (<b>a</b>) 4 h, (<b>b</b>) 8 h.</p>
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<p>SEM images of zeolite crystals for Experiment 4. (<b>a</b>) 4 h, (<b>b</b>) 8 h.</p>
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<p>Rietveld refinement plot: observed (+) and calculated profiles and difference plot for NaA zeolite (8 h, Experiment 2) and corundum NIST 676a with tick marks at the position of the Bragg peaks. From the bottom: NaA zeolite, corundum NIST 676a.</p>
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<p>IR spectrum of the sample at 8 h—Experiment 2.</p>
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<p>Raman spectra of the sample at 8 h—Experiment 2.</p>
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<p>Scheme of experimental protocol.</p>
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21 pages, 1951 KiB  
Article
A Farmer-Centric Cost–Benefit Analysis of Climate-Smart Agriculture in the Gandaki River Basin of Nepal
by Shobha Poudel, Rabin Thapa and Bhogendra Mishra
Climate 2024, 12(9), 145; https://doi.org/10.3390/cli12090145 (registering DOI) - 16 Sep 2024
Abstract
Climate-smart agriculture (CSA) is a climate-resilient practice that stands out globally as an important practice through which we can deal with emerging challenges through adaptation and mitigation to increase crop productivity and resilience. Despite its significance, a comprehensive cost–benefit analysis of the adoption [...] Read more.
Climate-smart agriculture (CSA) is a climate-resilient practice that stands out globally as an important practice through which we can deal with emerging challenges through adaptation and mitigation to increase crop productivity and resilience. Despite its significance, a comprehensive cost–benefit analysis of the adoption of these practices has not yet been carried out. This study aims to bridge the knowledge gap between the cost and effectiveness of CSA practices adopted by small-scale farmers in growing rice, wheat and maize, the most staple crops in the Gandaki River Basin of Nepal. In this study, net present value (NPV), internal rate of return (IRR), benefit–cost (BC) ratio, net benefit investment (NK) ratio and payback period, along with the value of externalities (social and environmental), were employed to assess the profitability of CSA practices. The findings indicate that almost all the CSA practices analyzed were profitable, with the exception of solar water management in maize with very low IRR (6%) and a longer payback period. The outcome of this study offers valuable insights for farmers in choosing profitable CSA technology and for policy makers in promoting better CSA technology, upscaling CSA practices, and formulating new agricultural policies and programs in the context of the changing climate. Full article
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<p>Conceptual framework of the study. Source: Author’s own.</p>
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<p>Map of the study area. Source: Author’s own.</p>
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<p>The productivity response function (the productivity increases in a linear plateau from t1 to t2, given one limiting factor, and then stabilizes over time when other factors limit production).</p>
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24 pages, 704 KiB  
Article
Impact of Foreign Direct Investment on Green Total Factor Productivity: New Evidence from Yangtze River Delta in China
by Shuai Chen, Jiameng Yang and Xue Chen
Sustainability 2024, 16(18), 8085; https://doi.org/10.3390/su16188085 (registering DOI) - 16 Sep 2024
Viewed by 103
Abstract
China has entered a period of high-quality development. As an important feature of high-quality development, green total factor productivity (GTFP) has attracted much attention. With the opening-up and economic globalization, the Yangtze River Delta, one of the strongest and most technological regions in [...] Read more.
China has entered a period of high-quality development. As an important feature of high-quality development, green total factor productivity (GTFP) has attracted much attention. With the opening-up and economic globalization, the Yangtze River Delta, one of the strongest and most technological regions in China, has been attracting an increasing amount of foreign direct investment (FDI). This study investigates if FDI is conducive to GTFP under the constraints of specific resources and a specific environment, which has important practical significance for the utilization of FDI in the Yangtze River Delta and China. Through a literature review and sorting the current FDI in the Yangtze River Delta, the GTFP and its decomposition indicators of 27 cities from 2004 to 2019 are calculated based on their energy consumption and pollution. Using the fixed-effects model and threshold model of panel data, this study tests whether FDI promotes GTFP and whether a nonlinear impact of FDI on GTFP exists. It finds that (1) the GTFP of most cities in the Yangtze River Delta improved during the sample period, but their annual growth declined. Technology is the dominant factor affecting the growth of GTFP. (2) FDI in the Yangtze River Delta has increased, and the investment structure has improved, but the distribution among cities is uneven. (3) The scale and quality of FDI have a positive impact on GTFP, which supports the “Pollution Halo” hypothesis. Economics, education, networks, and trade openness can promote the growth of GTFP, while environmental regulation, government intervention, and industrialization have a negative impact. (4) The quality of FDI, economics, the industrial structure, the environmental regulation, and the internet are each a significant single threshold characteristic for the impact of FDI on GTFP. When one of these factors is lower than a certain threshold, FDI has less impact on GTFP. When one exceeds a certain threshold, FDI’s positive promotion effect on GTFP significantly improves. Based on the analysis, this study offers some suggestions. The government should improve the FDI selection mechanism based on realities, make appropriate environmental regulatory policies, strengthen the construction of networks, and improve the “Internet+” effect on productivity. Full article
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<p>GML and its decomposed indexes trend in Yangtze River Delta from 2004 to 2019.</p>
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20 pages, 1843 KiB  
Article
Exploring Ecological Quality and Its Driving Factors in Diqing Prefecture, China, Based on Annual Remote Sensing Ecological Index and Multi-Source Data
by Chen Wang, Qianqian Sheng and Zunling Zhu
Land 2024, 13(9), 1499; https://doi.org/10.3390/land13091499 - 15 Sep 2024
Viewed by 209
Abstract
The interaction between the natural environmental and socioeconomic factors is crucial for assessing the dynamics of plateau ecosystems. Therefore, the remote sensing ecological index (RSEI) and CatBoost-SHAP model were employed to investigate changes in the ecological quality and their driving factors in the [...] Read more.
The interaction between the natural environmental and socioeconomic factors is crucial for assessing the dynamics of plateau ecosystems. Therefore, the remote sensing ecological index (RSEI) and CatBoost-SHAP model were employed to investigate changes in the ecological quality and their driving factors in the Diqing Tibetan Autonomous Prefecture, China, from 2001 to 2021. The results showed an increase from 0.44 in 2001 to 0.71 in 2021 in the average RSEI for the Diqing Prefecture, indicating an overall upward trend in the ecological quality. Spatial analysis shows the percentage of the area covered by different levels of RSEI and their temporal changes. The results revealed that “good” ecological quality accounted for the largest proportion of the study area, at 42.77%, followed by “moderate” at 21.93%, and “excellent” at 16.62%. “Fair” quality areas accounted for 16.11% and “poor” quality areas only 2.57%. The study of ecological and socioeconomic drivers based on the CatBoost-SHAP framework also indicated that natural climate factors have a greater impact on ecological quality than socioeconomic factors; however, this effect differed significantly with altitude. The findings suggest that, in addition to strengthening climate monitoring, further advancements in ecological engineering are required to ensure the sustainable development of the ecosystem and the continuous improvement of the environmental quality in the Diqing Prefecture. Full article
27 pages, 10973 KiB  
Article
Integrating Technological Environmental Design and Energy Interventions in the Residential Building Stock: The Pilot Case of the Small Island Procida
by Giada Romano, Serena Baiani and Francesco Mancini
Sustainability 2024, 16(18), 8071; https://doi.org/10.3390/su16188071 (registering DOI) - 15 Sep 2024
Viewed by 299
Abstract
The next decade will see severe environmental and technological risks, pushing our adaptive capacity to its limits. The EPBD Case Green directive, to counter this phenomenon, emphasizes accelerating building renovations, reducing GHG emissions and energy consumption, and promoting renewable energy installations. Additionally, it [...] Read more.
The next decade will see severe environmental and technological risks, pushing our adaptive capacity to its limits. The EPBD Case Green directive, to counter this phenomenon, emphasizes accelerating building renovations, reducing GHG emissions and energy consumption, and promoting renewable energy installations. Additionally, it calls for deadlines to phase out fossil fuels and mandates solar system installations. This research provides a comprehensive perspective on the opportunities for and challenges of incorporating renewable energy into the built environment. It focuses on the 2961 residential buildings on Procida, a small island located south of Italy, to efficiently utilize energy resources and lay the groundwork for sustainability. Beginning with an analysis of the territorial, urban, historical–conservation, structural, and geological context, in addition to environmental assessments, the research develops a classification and archetypalization system using in-house software. This system aggregates data on the island’s residential buildings, analyzes their current state, and formulates various intervention scenarios. These scenarios demonstrate how integrating technological–environmental design interventions, such as upgrading the building envelope and enhancing bioclimatic behavior, with energy retrofitting measures, such as replacing mechanical systems and installing solar panels, can improve the overall performance of the existing building stock and achieve energy self-sufficiency. Full article
(This article belongs to the Special Issue Renewable Energies in the Built Environment)
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<p>Framework of the research methodology.</p>
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<p>Number of dwellings divided into building construction period.</p>
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<p>Number of residential buildings divided by average size.</p>
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<p>Number of residential buildings divided into number of floors above ground level.</p>
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<p>Occupancy of residential buildings.</p>
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<p>(<b>left</b>) Typology of heating systems; (<b>right</b>) cooling systems.</p>
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<p>Typology of domestic hot water production systems.</p>
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<p>Tuff used in masonry according to two main techniques: the so-called “<span class="html-italic">a cantieri</span>” technique (<b>on the left</b>); and the so-called “<span class="html-italic">a blocchetti</span>” technique (<b>on the right</b>).</p>
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<p>(<b>left</b>) Example of masonry with “<span class="html-italic">a cantieri</span>” construction; (<b>right</b>) stratigraphy of the masonry from the exterior to the interior.</p>
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<p>(<b>left</b>) Example of masonry with “<span class="html-italic">a blocchetti</span>” construction; (<b>right</b>) stratigraphy of the masonry from the exterior to the interior.</p>
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<p>(<b>left</b>) Shading of the area on 21 June; (<b>right</b>) shading of the area on 21 December.</p>
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<p>(<b>left</b>) Shading of the area on 21 June; (<b>right</b>) shading of the area on 21 December.</p>
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<p>(<b>left</b>) Shading of the area on 21 June; (<b>right</b>) shading of the area on 21 December.</p>
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<p>Identification of archetypes on the island plan.</p>
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<p>Frequency of suggested interventions in percentages for the different size categories for reducing primary energy consumption.</p>
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<p>Frequency of suggested interventions in percentages for the different archetypes for primary energy reduction.</p>
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<p>Frequency of suggestion of interventions in total percentages for primary energy reduction.</p>
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<p>Comparative evaluation of intervention scenarios in terms of energy demand and associated CO<sub>2</sub> emissions divided by dwelling size.</p>
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<p>Comparative evaluation of intervention scenarios in terms of energy demand and associated CO<sub>2</sub> emissions divided by archetype.</p>
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25 pages, 1889 KiB  
Systematic Review
Factors Affecting Adherence to a Low Phenylalanine Diet in Patients with Phenylketonuria: A Systematic Review
by Roza Yagudina, Andrey Kulikov, Vyacheslav Serpik, Marina Protsenko and Kirill Kopeyka
Nutrients 2024, 16(18), 3119; https://doi.org/10.3390/nu16183119 (registering DOI) - 15 Sep 2024
Viewed by 276
Abstract
Phenylketonuria (PKU) is an inherited metabolic disorder that requires lifelong adherence to a low-phenylalanine (Phe) diet to prevent severe neurological complications. However, maintaining dietary adherence can be challenging for patients and their families. This systematic review aimed to comprehensively evaluate the factors affecting [...] Read more.
Phenylketonuria (PKU) is an inherited metabolic disorder that requires lifelong adherence to a low-phenylalanine (Phe) diet to prevent severe neurological complications. However, maintaining dietary adherence can be challenging for patients and their families. This systematic review aimed to comprehensively evaluate the factors affecting adherence to a low-Phe diet in patients with PKU. A systematic search of multiple databases was conducted, and 49 studies were included in the final analysis. The quality of evidence was assessed using the Joanna Briggs Institute levels of evidence and the Quality Assessment with Diverse Studies tool. The review identified four main categories of factors influencing dietary adherence: family-related factors (social, psychological, behavioral, and educational), patient-specific factors (psychological, behavioral, educational, and demographic), environmental factors (healthcare professional support, educational and camp-based interventions, and the COVID-19 pandemic), and therapy-related factors (protein substitute formulation, clinic visits, blood tests, and telemedicine). The findings highlight the complex interplay between elements contributing to dietary adherence in PKU patients and underscore the importance of a multifaceted approach to support patients and their families. Future research should prioritize high-quality longitudinal and experimental studies to provide stronger evidence for the PKU community. Full article
(This article belongs to the Special Issue Nutritional Management of Patients with Inborn Errors of Metabolism)
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<p>A flow diagram illustrating the search and selection process.</p>
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<p>A summary of facilitators and barriers to dietary adherence.</p>
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28 pages, 2653 KiB  
Article
How Does Digital Transformation Moderate Green Culture, Job Satisfaction, and Competitive Advantage in Sustainable Hotels?
by Gul Coskun Degirmen, Derya Ozilhan Ozbey, Emine Sardagı, Ilknur Cevik Tekin, Durmus Koc, Pınar Erdogan, Feden Koc and Emel Arık
Sustainability 2024, 16(18), 8072; https://doi.org/10.3390/su16188072 (registering DOI) - 15 Sep 2024
Viewed by 299
Abstract
Target groups within an organization adopt its culture, reflecting it in all internal and external business processes. Adopting a green organizational culture in hotels with sustainability certificates plays an important role in reshaping business processes by developing sustainability awareness among employees. Digital transformation, [...] Read more.
Target groups within an organization adopt its culture, reflecting it in all internal and external business processes. Adopting a green organizational culture in hotels with sustainability certificates plays an important role in reshaping business processes by developing sustainability awareness among employees. Digital transformation, which facilitates corporate culture and business processes, plays a role in employee job satisfaction while also supporting environmental, social, and economic sustainability. This research aims to determine the relationship between green organizational culture, job satisfaction, and competitive advantage variables and to examine the moderating role of digital transformation on these relationships. The data-collecting techniques of choice were surveys and semi-structured interviews. While Amos software (Version 24) was used to test the hypothetical model in the analysis of survey data, a Hayes Process macro was used to determine the moderating effect. The interview forms’ data was analyzed using a bag-of-words model. According to the research results, there is a positive relationship between the participation, consistency, and adaptability sub-dimensions of green organizational culture and job satisfaction, while there is no significant relationship between the mission sub-dimension and job satisfaction. Furthermore, the study reveals the moderating role of digital transformation in the effect of job satisfaction on competitive advantage. Full article
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<p>Hypothetical research model.</p>
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<p>Quantitative analysis flowchart.</p>
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<p>Qualitative analysis flowchart.</p>
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<p>Structural model coefficients. * <span class="html-italic">p</span> &lt; 0.001; ** <span class="html-italic">p</span> &lt; 0.05; N.S. not significant.</p>
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<p>Annotated corpus map.</p>
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<p>Word cloud after preprocessing.</p>
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18 pages, 3680 KiB  
Article
Innovative PEEK in Dentistry of Enhanced Adhesion and Sustainability through AI-Driven Surface Treatments
by Mattew A. Olawumi, Francis T. Omigbodun, Bankole I. Oladapo, Temitope Olumide Olugbade and David B. Olawade
Bioengineering 2024, 11(9), 924; https://doi.org/10.3390/bioengineering11090924 (registering DOI) - 14 Sep 2024
Viewed by 492
Abstract
This research investigates using Polyether ether ketone (PEEK) in dental prosthetics, focusing on enhancing the mechanical properties, adhesion capabilities, and environmental sustainability through AI-driven data analysis and advanced surface treatments. The objectives include improving PEEK’s adhesion to dental types of cement, assessing its [...] Read more.
This research investigates using Polyether ether ketone (PEEK) in dental prosthetics, focusing on enhancing the mechanical properties, adhesion capabilities, and environmental sustainability through AI-driven data analysis and advanced surface treatments. The objectives include improving PEEK’s adhesion to dental types of cement, assessing its biocompatibility, and evaluating its environmental impact compared to traditional materials. The methodologies employed involve surface treatments such as plasma treatment and chemical etching, mechanical testing under ASTM standards, biocompatibility assessments, and lifecycle analysis. AI models predict and optimize mechanical properties based on extensive data. Significant findings indicate that surface-treated PEEK exhibits superior adhesion properties, maintaining robust mechanical integrity with no cytotoxic effects and supporting its use in direct contact with human tissues. Lifecycle analysis suggests PEEK offers a reduced environmental footprint due to lower energy-intensive production processes and recyclability. AI-driven analysis further enhances the material’s performance prediction and optimization, ensuring better clinical outcomes. The study concludes that with improved surface treatments and AI optimization, PEEK is a promising alternative to conventional dental materials, combining enhanced performance with environmental sustainability, paving the way for broader acceptance in dental applications. Full article
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<p>Advanced surface modification of PEEK for enhanced dental implants.</p>
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<p>Stress–strain behaviour of PEEK compared to traditional dental materials.</p>
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<p>(<b>a</b>) Fatigue resistance of PEEK under cyclic loading; (<b>b</b>) wear resistance of PEEK under simulated oral conditions; (<b>c</b>) biocompatibility indicators of PEEK over time; and (<b>d</b>) microbial colonisation on PEEK and titanium surfaces.</p>
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<p>Bar graph showing the thermal conductivity of PEEK compared to other dental materials such as titanium, zirconia, and composite resin.</p>
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<p>(<b>a</b>) Line graph depicting the fatigue resistance of PEEK under cyclic loading conditions; (<b>b</b>) line graph showing the wear resistance of PEEK under simulated oral conditions; (<b>c</b>) line graph displaying long-term biocompatibility indicators, tracking inflammation markers and tissue integration over time; (<b>d</b>) bar graph comparing microbial colonization rates on PEEK and titanium surfaces.</p>
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<p>(<b>a</b>) Bar graph showing surface energy comparisons before and after surface treatments on PEEK; (<b>b</b>) graph displaying the impact of surface treatment on the bond strength of PEEK to dental types of cement; (<b>c</b>) line graph illustrating the effect of aging on the mechanical properties of PEEK in dental applications; (<b>d</b>) patient satisfaction scores statistical representations comparing patient satisfaction related to comfort, function, and aesthetics of PEEK versus other materials.</p>
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<p>(<b>a</b>) Surface energy comparison before and after surface treatments on PEEK; (<b>b</b>) impact of surface treatment on the bond strength of PEEK to dental types of cement; (<b>c</b>) effect of aging on the mechanical properties of PEEK in dental applications; and (<b>d</b>) patient satisfaction scores with PEEK-based prosthetics vs. traditional materials.</p>
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<p>(<b>a</b>) Thermal stability charts; (<b>b</b>) water absorption curves; (<b>c</b>) fatigue cycle graphs.</p>
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