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13 pages, 436 KiB  
Article
Effects of Mayonnaise and Salad Dressing Marinades on Chicken Breast Meat Quality
by Jiale Liang, Nana Sarudate, Hazuki Otsuki, Takuya Yanagisawa, Yusuke Okuda and Shin-ichi Ishikawa
Gastronomy 2024, 2(3), 116-128; https://doi.org/10.3390/gastronomy2030009 - 29 Aug 2024
Viewed by 285
Abstract
Mayonnaise has a long history as a representative of emulsified sauces. As people become more health-conscious, salad dressings (emulsified and non-emulsified) with lower fat content gradually appear in people’s lives. Both sauces are widely used in everyday life for meat marinades because they [...] Read more.
Mayonnaise has a long history as a representative of emulsified sauces. As people become more health-conscious, salad dressings (emulsified and non-emulsified) with lower fat content gradually appear in people’s lives. Both sauces are widely used in everyday life for meat marinades because they contain seasonings such as spices, salt, and vinegar. Although there are many studies on how condiments such as spices, salt, and vinegar affect meat, the effects of semi-solid/liquid emulsions and non-emulsified marinades on meat have yet to be further discussed and analyzed. Therefore, studying the physical and chemical effects of mayonnaise (semi-solid emulsified emulsion) and salad dressing (liquid emulsion and non-emulsified sauce) on meat is essential for improving food quality and safety. Thus, this paper examines the impacts of mayonnaise, emulsified salad dressing, and non-emulsified salad dressing on the physicochemical properties and sensory evaluations of meat. The results showed that the three sauces effectively reduced cooking losses when used as marinades for chicken breasts. In the juiciness and firmness tests, both mayonnaise and non-emulsified salad dressings positively affected the meat. This study also found that lower pH values were not always effective at reducing meat hardness and that emulsification may play a key role in reducing meat hardness. Full article
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<p>Measurement of pH. A Point, 5 mm from the surface of the chicken; B Point, midway between the A point and the center point; C Point, the center point.</p>
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13 pages, 5728 KiB  
Article
Revealing the Genetic Diversity and Population Structure of Garlic Resource Cultivars and Screening of Core Cultivars Based on Specific Length Amplified Fragment Sequencing (SLAF-Seq)
by Jing Yang, Meile Sun, Xiangrong Ren, Pengbing Li, Jingtao Hui, Jun Zhang and Guocang Lin
Genes 2024, 15(9), 1135; https://doi.org/10.3390/genes15091135 - 28 Aug 2024
Viewed by 345
Abstract
Garlic is an important vegetable and condiment that has good medical and health care effects. At present, the origin of Chinese garlic and its association with other types of quality are limited to the molecular marker level, and there are few reports at [...] Read more.
Garlic is an important vegetable and condiment that has good medical and health care effects. At present, the origin of Chinese garlic and its association with other types of quality are limited to the molecular marker level, and there are few reports at the genome level. Therefore, this study is based on the specific length amplified fragment sequencing (SLAF-seq) of 102 copies of garlic germplasm resources, the group structure, and further screening of the core germplasm. SLAF-seq of 102 garlic cultivars yielded 1949.85 Mb of clean data and 526,432,275 SNPs. Through principal component analysis, evolutionary tree, population structure, and genetic relationship analysis, all garlic cultivars were divided into 3 groups. Among them, Group 1 contains 45 Chinese cultivars and 1 Egyptian cultivar, which are distributed mainly in the coastal and central areas of China. Group 2 contains 36 Chinese cultivars and 1 U.S. cultivar, which are distributed mainly in Northwest China. Group 3 contains 19 Chinese cultivars, which are distributed mainly in Xinjiang, China. The genetic diversity results indicate that the fixation index (Fst) values of Group 1 and Group 2 are lower than those of Group 1 and Group 3 and that the diversity of nucleotides (π) of Group 3 is greater than those of Group 2 and Group 1. Finally, the 30 parts of the cultivars were used as the core germplasms, and there was no difference between the two cultivars in terms of core quality. In summary, this study provides tags for the determination of garlic molecular markers and genotypes and provides a theoretical basis for subsequent resource protection and utilization, genetic positioning of important agronomic traits, and molecular marking agglomeration breeding. Full article
(This article belongs to the Special Issue Abiotic Stress in Plants: Genetics and Genomics)
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<p>Distribution of SNPs on chromosomes. The abscissa is the length of the chromosome; each band represents a chromosome, and the genome is divided according to a size of 1 Mb. The more SNP markers present in each window, the darker red the color, and the fewer SNP markers present, the lighter green the color. Therefore, the darker red area in the figure is the area where the SNP markers are concentrated.</p>
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<p>(<b>a</b>) Phylogenetic tree of 102 garlic samples; different colors represent different groups. (<b>b</b>) PCA of 102 garlic samples; different colors represent different groups.</p>
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<p>(<b>a</b>) Proportion of 102 servings of garlic ingredients; different colors represent different groups. (<b>b</b>) Geographical distribution of 100 Chinese garlic cultivars, with different colors representing different groups.</p>
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<p>(<b>a</b>) Cross-validation error rate of 102 garlic samples with K values of 1–10; (<b>b</b>) clustering of 102 garlic samples with K = 3. (<b>c</b>) Cluster heatmaps of 102 garlic samples.</p>
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<p>Nucleotide diversity and genetic differentiation coefficient between groups.</p>
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<p>(<b>a</b>) Gene coverage (CV) evaluation. The abscissa represents the number of samples corresponding to the proportion of selected germplasm resources, the ordinate represents the CV value, and the red dots in the graph represent the candidate core germplasm. (<b>b</b>) Proportional distribution of genotypes, with the abscissa representing the ten genotypes; the ordinate represents the proportion of each genotype, the red dotted line represents the genotype distribution of all germplasm resources, and the blue dotted line represents the genotype distribution of the selected core germplasm. (<b>c</b>) Proportion of core garlic material; different colors represent different groups. (<b>d</b>) Geographical distribution of the core garlic material, with different colors representing different groupings.</p>
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14 pages, 1690 KiB  
Article
Enrichment of White Wine Vinegar with Aromatic Plants: The Impact on Aromatic, Polyphenolic, and Sensory Profiles
by Marin Krapac, Nikola Major, Tomislav Plavša, Ana Jeromel, Ivana Tomaz and Danijela Poljuha
Appl. Sci. 2024, 14(16), 6909; https://doi.org/10.3390/app14166909 - 7 Aug 2024
Viewed by 786
Abstract
The food industry is developing intensively, and products that, with their characteristics, enrich the food taste and aroma are widely used in the culinary arts. White wine vinegar is often used as a food condiment and as dressing in salads. This research aims [...] Read more.
The food industry is developing intensively, and products that, with their characteristics, enrich the food taste and aroma are widely used in the culinary arts. White wine vinegar is often used as a food condiment and as dressing in salads. This research aims to explore the impact of the maceration of selected aromatic plants on the organoleptic properties, bioactive compounds, and sensory profile of white wine vinegar. The plants selected for white wine (cv ‘Malvazija istarska’) vinegar aromatization were rosemary (Rosmarinus officinalis L.) and thyme (Thymus serpyllum L.) dried leaves and black elder (Sambucus nigra L. ssp. nigra) flowers (elderflowers). Vinegar flavored with rosemary had more pronounced pinewood and herbal aromas, while spicy aromas dominated the vinegar with thyme. The elderflower-flavored vinegar, on the other hand, was characterized by a floral and fruity aroma. Among the analyzed vinegars, white wine vinegar flavored with elderflower had the highest polyphenolic content. Full article
(This article belongs to the Special Issue Natural Products and Bioactive Compounds)
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<p>Scheme of vinegar production and analysis.</p>
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<p>Differences in the odorant series (herbal, floral, fruity, sweet, spicy, camphor-like, and unpleasant) of the flavored vinegars (shown as a bar graph) compared to the control treatment (shown on the <span class="html-italic">x</span>-axis) expressed as percentage changes. The odor series is represented by the sum of mean values of all compounds of the same odorant series where a significant difference between treatments was determined.</p>
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<p>Principal component analysis (PCA) for vinegars: (<b>a</b>) treatments (cases); (<b>b</b>) aromatic groups—blue letters; phenolic groups—green letters (variables). Abbreviations: Alc—alcohols, Acd—acids, Est—esters, Ter—terpenes, Otha—miscellaneous aromas, Ant—anthocyanins, Fla—flavones, FlaOL—flavonols, Fla-3-ol—flavan-3-ol, Dhf—dihydroflavonols, HbA—hydroxybenzoic acids, HcA—hydroxycinnamic acids, HppA—hydroxyphenylpropanoic acids, Stil—stilbenes, Hba—hydroxybenzaldehydes, Ty—tyrosols.</p>
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<p>Comparison of the odor properties of flavored vinegars (elderflower, rosemary, and thyme) and the control treatment. An analysis of variance (ANOVA) was performed to determine if there was a significant difference among the odor properties of the vinegars. Odor properties marked with asterisks (**, <span class="html-italic">p</span> ≤ 0.01; ***, <span class="html-italic">p</span> ≤ 0.001) show significant differences.</p>
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11 pages, 794 KiB  
Article
The Relationship between Low-Sodium Salt Intake and Both Blood Pressure Level and Hypertension in Chinese Residents
by Cuicui Wang, Zilong Lu, Jiyu Zhang, Xiaorong Chen, Jianwei Xu, Bingyin Zhang, Jing Dong, Jie Ren, Chunxiao Xu, Congcong Gao, Xiaolei Guo, Jing Wu and Jixiang Ma
Nutrients 2024, 16(12), 1909; https://doi.org/10.3390/nu16121909 - 17 Jun 2024
Viewed by 920
Abstract
Compared to common salt, low-sodium salt can reduce blood pressure to varying degrees. However, the exact dosage relationship remains unclear. We aimed to investigate the dose–response relationships between low-sodium salt intake and systolic blood pressure (SBP) and diastolic blood pressure (DBP), as well [...] Read more.
Compared to common salt, low-sodium salt can reduce blood pressure to varying degrees. However, the exact dosage relationship remains unclear. We aimed to investigate the dose–response relationships between low-sodium salt intake and systolic blood pressure (SBP) and diastolic blood pressure (DBP), as well as the risk of hypertension, and to determine the optimal range for low-sodium salt intake. We investigated the basic characteristics and dietary profile of 350 individuals who consumed low-sodium salt. The samples were divided into three groups according to the 33.3rd and 66.6th percentiles of low-sodium salt intake in condiments (Q1: <4.72 g/d, Q2: ≥4.72 g/d, and <6.88 g/d, and Q3: ≥6.88 g/d). The restricted cubic spline results indicated that low-sodium salt intake decreased linearly with SBP and DBP, while low-sodium intake demonstrated a non-linear, L-shaped relationship with the risk of hypertension, with a safe range of 5.81 g to 7.66 g. The multiple linear regression analysis revealed that compared with group Q1, the DBP in group Q2 decreased by 2.843 mmHg (95%CI: −5.552, −0.133), and the SBP in group Q3 decreased by 4.997 mmHg (95%CI: −9.136, −0.858). Exploratory subgroup analyses indicated that low-sodium salt intake had a significant impact on reducing SBP in males, DBP in females, SBP in rural populations, and DBP in urban populations. The intake of low-sodium salt adheres to the principle of moderation, with 5.81–7.66 g potentially serving as a pivotal threshold. Full article
(This article belongs to the Section Micronutrients and Human Health)
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<p>(<b>a</b>) The relationship between low−sodium salt intake and SBP; (<b>b</b>) the relationship between low−sodium salt intake and DBP; (<b>c</b>) the relationship between low−sodium salt intake and the risk of hypertension. The restricted cubic spline model of the relationship model was adjusted for age, gender, region, marital status, education level, income, smoking status, drinking status, physical exercise, BMI, dyslipidemia, diabetes, and central obesity. The horizontal dashed line represents the case where β = 0 or OR = 1, while the vertical dashed line indicates the vertical line through the point (the point where the red solid line intersects the horizontal dashed line).</p>
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14 pages, 1996 KiB  
Article
Exploring the In Vitro Antioxidant, Anti-Aging, and Cytotoxic Properties of Kaempferia galanga Linn. Rhizome Extracts for Cosmeceutical Formulations
by Panikchar Wichayapreechar, Ranit Charoenjittichai, Anchalee Prasansuklab, Maria Pilar Vinardell and Wandee Rungseevijitprapa
Cosmetics 2024, 11(3), 97; https://doi.org/10.3390/cosmetics11030097 - 13 Jun 2024
Viewed by 1054
Abstract
Kaempferia galanga Linn. (KG), a member of the family Zingiberaceae, is native to India, and commonly found in China, Indonesia, and Thailand. It has been used as a food condiment, folk medicine, and to relieve skin diseases due to its biological activities. However, [...] Read more.
Kaempferia galanga Linn. (KG), a member of the family Zingiberaceae, is native to India, and commonly found in China, Indonesia, and Thailand. It has been used as a food condiment, folk medicine, and to relieve skin diseases due to its biological activities. However, its anti-aging effect has not yet been investigated. In this study, the rhizome of Kaempferia galanga Linn was extracted with solvents of different polarities (deionized water, absolute ethanol, ethyl acetate, and hexane). Phytochemical screening assay, total flavonoid and total phenolic contents, antioxidant activity (DPPH, FRAP, ABTS +• assay), anti-aging activity (anti-collagenase, anti-elastase), and cell cytotoxicity on human dermal fibroblasts were investigated. The outcomes revealed that the extraction in highly polar solvents resulted in a high extract yield. Flavonoids, phenolic, and terpenoid compounds were detected in KG extracts using all extraction solvents. However, deionized water as a solvent exhibited the lowest level of flavonoids and phenolics, as compared to the other solvents. The highest total flavonoid and phenolic contents were achieved through extraction with absolute ethanol and ethyl acetate, respectively. Interestingly, the extract obtained with absolute ethanol exhibited the most potent antioxidant activities (the IC50 value of DPPH was 0.612 mg/mL, the FRAP value was 62.79 mmol of Fe2+/g of extract, and TEAC value was 9.21 mg TE/g of extract in ABTS+• assay) and anti-aging properties (the percentages of collagenase inhibitory and elastase were 71.83%, and 66.35%, respectively). Regarding cell cytotoxicity, both KG extracts obtained with deionized water and absolute ethanol showed lower toxicity on human dermal fibroblasts compared to those obtained with ethyl acetate and hexane. Ethanol-based KG extract demonstrated a good antioxidant, anti-aging capacity and is considered safe for cosmeceutical products focused on anti-aging applications. Full article
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<p><span class="html-italic">Kaempferia galanga</span> Linn. (KG). (<b>A</b>) Rhizome of KG; (<b>B</b>) small pieces of KG rhizome; (<b>C</b>) finely powdered KG.</p>
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<p>Percentage of extraction yield. <span class="html-italic">Kaempferia galanga</span> Linn. (<span class="html-italic">KG</span>) extracted by maceration method with different polarity solvents.</p>
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<p>Collagenase inhibitory activity of KG extracts obtained with solvents of different polarities. The final concentration of the tested samples was 0.5 mg/mL. The values are expressed as the mean ± SD in triplicate experiments. Different alphabet letters indicate a statistically significant difference between solvents (<span class="html-italic">p</span> &lt; 0.05).</p>
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<p>Elastase inhibitory activity of KG extracts obtained with solvents of different polarities. The final concentration of the tested samples was 0.5 mg/mL. The values are expressed as the mean ± SD in triplicate experiments. Different alphabet letters indicate a statistically significant difference between solvents (<span class="html-italic">p</span> &lt; 0.05).</p>
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<p>The cytotoxicity of KG extracts obtained with solvents of different polarity on human fibroblast cells (WS-1). The values are expressed as the mean ± SD in triplicate experiments. The asterisk mark (*) indicates a statistically significant difference at <span class="html-italic">p</span>  &lt;  0.05 compared to the control group. Different alphabet letters indicate a statistically significant difference at <span class="html-italic">p</span> &lt; 0.05 among the different solvents.</p>
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13 pages, 811 KiB  
Article
An Exploration of the Gastronomic Potential of the North American Pawpaw—A Case Study from the Pawpaw Cookoff at the Ohio Pawpaw Festival
by Robert Brannan and Ronald Powell
Gastronomy 2024, 2(2), 89-101; https://doi.org/10.3390/gastronomy2020007 - 13 Jun 2024
Viewed by 634
Abstract
The pawpaw (Asimina triloba) is a tropical-tasting fruit from a tree indigenous to North America’s temperate climates. Pawpaw has a sweet, sour, and often bitter taste, which is a combination of banana and mango flavor. Fresh fruits are rarely available for [...] Read more.
The pawpaw (Asimina triloba) is a tropical-tasting fruit from a tree indigenous to North America’s temperate climates. Pawpaw has a sweet, sour, and often bitter taste, which is a combination of banana and mango flavor. Fresh fruits are rarely available for retail purchase other than at farm sales or farmers’ markets. Frozen pulp is commercially available but supply is often limited due to increased wholesale demand. The purpose of this paper is to analyze seven years of entries from the Pawpaw Cookoff at the Ohio Pawpaw Festival using case study methodology to provide a basis from which to track trends, make recommendations, and discuss gastronomic opportunities of the pawpaw. Analysis of the data indicates that pawpaw is a versatile fruit that has been incorporated into a wide variety of beverages, savories, sauces, condiments, sweets, and desserts. Pawpaw pairs well with both alcoholic and non-alcoholic beverages, especially certain ales, sour beers, innovative cocktails with rum, coffee and tea drinks, and smoothies. Pawpaw is easily incorporated directly into salsas, dips, salad dressings, and frozen desserts that do not require cooking. In cooked savory dishes, sauces, and condiments, pawpaw should be added at the end of preparation. Pawpaw can be used in a wide range of baked and fried sweets and desserts. Full article
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<p>Typical ripe (<b>top</b>) and overripe (<b>bottom</b>) North American pawpaws (<span class="html-italic">Asimina triloba</span>) of unidentified variety. (Adapted from <a href="https://commons.wikimedia.org/wiki/File:Ripe_fruit_of_Asimina_triloba.jpg" target="_blank">https://commons.wikimedia.org/wiki/File:Ripe_fruit_of_Asimina_triloba.jpg</a> (<b>top</b>) and <a href="https://commons.wikimedia.org/wiki/File:Pawpaw_ripe_fruit_green_to_brown.jpg" target="_blank">https://commons.wikimedia.org/wiki/File:Pawpaw_ripe_fruit_green_to_brown.jpg</a> (<b>bottom</b>) (Accessed on 7 March 2024). These files are licensed under the Creative Commons Attribution-Share Alike 4.0 International license).</p>
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<p>Percentage of beers categorized into five general style categories, each based on one or more Beer Judge Certification Program, Inc. classifications.</p>
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17 pages, 1320 KiB  
Article
Relationship between Household Shared Meal Frequency and Dietary Intake among Men and Women Aged ≥20 Years: Cross-Sectional Analyses Based on 2018 and 2019 National Health and Nutrition Surveys in Japan
by Xiaoyi Yuan, Mai Matsumoto, Emiko Okada, Kentaro Murakami, Satoshi Sasaki and Hidemi Takimoto
Nutrients 2024, 16(11), 1764; https://doi.org/10.3390/nu16111764 - 4 Jun 2024
Viewed by 920
Abstract
This study aimed to examine the relationship between the frequency of household shared meals and the intake of 17 food groups and 21 nutrients. Participants were 3310 men and 3386 women ≥20 years old living in a household of ≥2 members from 2018 [...] Read more.
This study aimed to examine the relationship between the frequency of household shared meals and the intake of 17 food groups and 21 nutrients. Participants were 3310 men and 3386 women ≥20 years old living in a household of ≥2 members from 2018 and 2019 National Health and Nutrition Surveys in Japan. A one-day household dietary record was used to classify an individual’s shared meal frequency and dietary intake. A shared meal is defined as an eating occasion (i.e., breakfast, lunch, and dinner) where ≥1 food item—other than sugars, fats and oils, beverages, and condiments—was recorded with an assigned approximated shared proportion. The shared meal frequency for each individual was classified into one of four categories: 0, 1, 2, and 3 times/day. Dietary intake was compared across the shared meal categories adjusted for age, occupation, household size, meal skipping, snacking, residential areas, and within-household correlations. Both men and women who had more frequent shared meals showed higher intakes of potatoes, vegetables, mushrooms, and condiments but less confectioneries and beverages. A higher shared meal frequency was also related to a higher intake of many (12/21) nutrients (e.g., protein, dietary fiber, and potassium). However, in women, there was a positive association between shared meal frequency and sodium intake. A higher frequency of household shared meals may be related to a more favorable dietary intake; except for concerns about sodium intake in women. Full article
(This article belongs to the Section Nutrition and Public Health)
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<p>Flowchart of the analytical sample of this study. * Excluded for calculating BMR to assess misreporting of energy intake.</p>
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<p>An example of the 1-day dietary record of breakfast to be filled by the meal preparers used in the National Health and Nutrition Survey in Japan. Intakes of the dishes ①②③ were assumed based on the approximated shared proportion method, which would later calculate the intake of the component ingredients. For example, the intake value of the food item Natto for HH member 1 would be calculated as 80 g (based on 40 g/pack) × 40% = 32 g. HH, household. The amount consumed means the total amount of food, not including waste or inedible parts (e.g., fish head, peels).</p>
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16 pages, 2866 KiB  
Article
Doenjang Ameliorates Diet-Induced Hyperlipidemia and Hepatic Oxidative Damage by Improving Lipid Metabolism, Oxidative Stress, and Inflammation in ICR Mice
by Olivet Chiamaka Edward, Do-Youn Jeong, Hee-Jong Yang, Anna Han and Youn-Soo Cha
Foods 2024, 13(10), 1471; https://doi.org/10.3390/foods13101471 - 10 May 2024
Viewed by 1101
Abstract
Hyperlipidemia, characterized by elevated cholesterol, lipids, and triglycerides in the bloodstream, is linked to hepatic oxidative damage. Doenjang, a traditional Korean condiment made from fermented soybeans, is known for its health benefits, yet its anti-hyperlipidemic effects remain understudied. Our study aimed to [...] Read more.
Hyperlipidemia, characterized by elevated cholesterol, lipids, and triglycerides in the bloodstream, is linked to hepatic oxidative damage. Doenjang, a traditional Korean condiment made from fermented soybeans, is known for its health benefits, yet its anti-hyperlipidemic effects remain understudied. Our study aimed to assess the hypolipidemic and hepatic protective effects of Doenjang on male ICR mice fed a high-fat cholesterol diet for 8 weeks. Mice were divided into three groups: the normal diet (ND), the high-fat cholesterol diet (HD), and the Doenjang-supplemented HD diet (DS) group. Doenjang supplementation significantly regulated total cholesterol, triglycerides, LDL cholesterol, and HDL cholesterol levels compared to the HD group. It also downregulated lipogenic genes, including PPARγ, FAS, and ACC, and positively influenced the cholesterol metabolism-related genes HMGCR and LXR. Moreover, Doenjang intake increased serum glutathione levels, activated oxidative stress defense genes (NRF2, SOD, GPx1, and CAT), positively modulated inflammation genes (NF-kB and IL6) in hepatic tissue, and reduced malondialdehyde levels. Our findings highlight the effectiveness of traditional Doenjang in preventing diet-induced hyperlipidemia and protecting against hepatic oxidative damage. Full article
(This article belongs to the Special Issue The Functional Foods: New Trends and Perspectives)
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<p>Effect of <span class="html-italic">Doenjang</span> intake on feed intake, body and tissue weights, and adipocyte morphology. (<b>A</b>) Daily food intake; (<b>B</b>) daily caloric intake; (<b>C</b>) food efficiency ratio; (<b>D</b>) body weight changes over the experiment period; (<b>E</b>) final body weight; (<b>F</b>) Lee index; (<b>G</b>) tissue weights (g) (subcutaneous fat tissue, epididymal fat tissue, and liver tissue weights); (<b>H</b>) relative tissue weight (g) (subcutaneous fat tissue, epididymal fat tissue, and liver tissue relative weights); (<b>I</b>) histological captures of epididymal fat tissue at a scale of 40 μm and the mean adipocyte area; (<b>J</b>) histological captures of subcutaneous fat tissue at a scale of 40 μm and the mean adipocyte area. Each bar represents the mean ± SE. A one-way ANOVA was used to find the statistical significance at <span class="html-italic">p</span> &lt; 0.05. Bars with different upper scripts differ significantly from each other.</p>
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<p>Effect of <span class="html-italic">Doenjang</span> on serum and hepatic lipid profiles and systolic blood pressure. (<b>A</b>) Serum total cholesterol levels; (<b>B</b>) serum total triglyceride levels; (<b>C</b>) high-density lipoprotein-cholesterol levels; (<b>D</b>) low-density lipoprotein-cholesterol levels; (<b>E</b>) very-low-density lipoprotein-cholesterol levels; (<b>F</b>) atherosclerosis index; (<b>G</b>) systolic blood pressure over the experiment period; (<b>H</b>) hepatic total triglyceride levels; (<b>I</b>) hepatic total cholesterol levels; (<b>J</b>) serum AST levels; (<b>K</b>) serum ALT levels. Each bar represents the mean ± SE. A one-way ANOVA was used to find statistical significance at <span class="html-italic">p</span> &lt; 0.05. Bars with different upper scripts differ significantly from each other.</p>
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<p>Effect of <span class="html-italic">Doenjang</span> on glucose metabolism, pancreatic histology, and mRNA expression of genes in pancreatic tissue. (<b>A</b>) Fasting glucose levels over the experiment period; (<b>B</b>) final fasting glucose levels in nmol/L; (<b>C</b>) serum insulin levels; (<b>D</b>) HOMA IR levels; (<b>E</b>) histological captures of pancreatic tissue at a scale of 20 μm and 40 μm (circled area shows fat deposits, arrow shows abnormal islets of Langerhans); (<b>F</b>) mRNA expression level of pancreatic Glut4; (<b>G</b>) mRNA expression level of pancreatic PGC-1α; (<b>H</b>) mRNA expression level of pancreatic G6P. Each bar represents the mean ± SE. A one-way ANOVA was used to find statistical significance at <span class="html-italic">p</span> &lt; 0.05. * indicates a significant difference according to an independent <span class="html-italic">t</span>-test at <span class="html-italic">p</span> &lt; 0.05. Bars with different upper scripts differ significantly from each other.</p>
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<p>Effect of <span class="html-italic">Doenjang</span> on oxidative stress parameters and mRNA expression levels relative to beta-actin of oxidative stress and inflammation-related genes and enzymes in hepatic tissue. (<b>A</b>) Serum glutathione (GSH) levels; (<b>B</b>) serum malondialdehyde (MDA) levels; (<b>C</b>) mRNA expression level of NRF2; (<b>D</b>) mRNA expression level of SOD; (<b>E</b>) mRNA expression level of GPx1; (<b>F</b>) mRNA expression level of CAT; (<b>G</b>) mRNA expression level of NF-kB; (<b>H</b>) mRNA expression level of IL6. Each bar represents the mean ± SE. A one-way ANOVA was used to find statistical significance at <span class="html-italic">p</span> &lt; 0.05. * indicates a significant difference according to an independent <span class="html-italic">t</span>-test at <span class="html-italic">p</span> &lt; 0.05. Bars with different upper scripts differ significantly from each other.</p>
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<p>Effect of <span class="html-italic">Doenjang</span> on hepatic histology and mRNA expression levels relative to beta-actin of lipid, triglyceride synthesis, and cholesterol metabolism-related genes and enzymes in hepatic tissue. (<b>A</b>) Histological captures of liver tissue at a scale of 40 μm and hepatic lipid droplet area; (<b>B</b>) mRNA expression level of SREBP1c; (<b>C</b>) mRNA expression level of ACC; (<b>D</b>) mRNA expression level of FAS; (<b>E</b>) mRNA expression level of PPARγ; (<b>F</b>) mRNA expression level of PPARα; (<b>G</b>) mRNA expression level of CPT1; (<b>H</b>) mRNA expression level of HMGCR; (<b>I</b>) mRNA expression level of LXR. Each bar represents the mean ± SE. A one-way ANOVA was used to find statistical significance at <span class="html-italic">p</span> &lt; 0.05. Bars with different upper scripts differ significantly from each other.</p>
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<p>Effect of <span class="html-italic">Doenjang</span> on hepatic protein expressions of lipid metabolism, cholesterol metabolism, and oxidative damage defense-related genes. (<b>A</b>) Western blot images of investigated genes; (<b>B</b>) protein expression of p-AMPK/AMPK; (<b>C</b>) protein expression of p-acc/acc; (<b>D</b>) protein expressions of SREBP1, PPARα, PPARγ, FAS, and FABP4 relative to β-actin; (<b>E</b>) protein expressions of SOD1, SOD2, CAT, GPx1, GPX2, and GPx1 homotetramer form relative to β-actin. Each bar represents the mean ± SE. A one-way ANOVA was used to find statistical significance at <span class="html-italic">p</span> &lt; 0.05. * indicates a significant difference according to an independent <span class="html-italic">t</span>-test at <span class="html-italic">p</span> &lt; 0.05, ** indicates a significant difference according to an independent <span class="html-italic">t</span>-test at <span class="html-italic">p</span> &lt; 0.01.</p>
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213 KiB  
Proceeding Paper
Organic Amendments for Growth, Yield and Quality of Green Coriander (Coriandrum sativum L.)
by Mausum Kumar Nath
Biol. Life Sci. Forum 2024, 30(1), 26; https://doi.org/10.3390/IOCAG2023-17342 - 18 Apr 2024
Viewed by 382
Abstract
Fresh and green leafy vegetables are an inevitable part of human nutrition. Leafy coriander is one of the most important condiments in the world and requires adequate fertilizer input for higher production. Expanding population constraints have compelled many countries to use pesticides and [...] Read more.
Fresh and green leafy vegetables are an inevitable part of human nutrition. Leafy coriander is one of the most important condiments in the world and requires adequate fertilizer input for higher production. Expanding population constraints have compelled many countries to use pesticides and fertilizers in order to boost farm production and fulfil their ever-increasing food demands. To stimulate the rapid and sumptuous growth of leafy vegetables like coriander, farmers apply a lot of nitrogenous fertilizers, resulting in poor quality and shelf lives. The application of organic amendments can solve this issue by improving the quality of coriander, as well as prolonging its shelf life. Moreover, various research work has been carried out in India and abroad on coriander as a seed spice, but limited research has been conducted on coriander as a condiment. Hence, this investigation was taken up. The experiment was prepared with seven treatments in a randomized block design and was replicated three times in the organic block of the Experimental Farm at Assam Agricultural University, India. The data from the respective field experiment were subjected to appropriate statistical analysis, as per the procedure proposed by Panse and Sukhatme. The result of the present investigation revealed that T7 (enriched compost @ 5 t ha−1) could produce the maximum yield with the highest benefit–cost ratio of 3.18, along with the best performance in terms of the quality of produce. Therefore, T7 can be inferred as farmer-friendly for sustainable production due to its efficiency, higher net return in comparison to other treatments, and minimal impact on the environment (and thus its adoption can be taken into consideration under field conditions). Full article
(This article belongs to the Proceedings of The 2nd International Online Conference on Agriculture)
16 pages, 2156 KiB  
Article
Influence of the Drying Process on the Volatile Profile of Different Capsicum Species
by Cosimo Taiti, Diego Comparini, Lavinia Moscovini, Simona Violino, Corrado Costa and Stefano Mancuso
Plants 2024, 13(8), 1131; https://doi.org/10.3390/plants13081131 - 18 Apr 2024
Cited by 1 | Viewed by 1043
Abstract
Chili is a globally significant spice used fresh or dried for culinary, condiment, and medicinal purposes. Growing concerns about food safety have increased the demand for high-quality products and non-invasive tools for quality control like origin tracing and safety assurance. Volatile analysis offers [...] Read more.
Chili is a globally significant spice used fresh or dried for culinary, condiment, and medicinal purposes. Growing concerns about food safety have increased the demand for high-quality products and non-invasive tools for quality control like origin tracing and safety assurance. Volatile analysis offers a rapid, comprehensive, and safe method for characterizing various food products. Thus, this study aims to assess the impact of the drying process on the aromatic composition of various Capsicum species and to identify key compounds driving the aromatic complexity of each genetic makeup. To accomplish these objectives, the aroma was examined in fruits collected from 19 different pepper accessions (Capsicum sp.) belonging to four species: one ancestral (C. chacoense) and three domesticated pepper species (C. annuum, C. baccatum and C. chinense). Fresh and dried samples were analyzed using a headspace PTR-TOF-MS platform. Our findings reveal significant changes in the composition and concentration of volatile organic compounds (VOCs) from fresh to dried Capsicum. Notably, chili peppers of the species C. chinense consistently exhibited higher emission intensity and a more complex aroma compared to other species (both fresh and dried). Overall, the data clearly demonstrate that the drying process generally leads to a reduction in the intensity and complexity of the aromatic compounds emitted. Specifically, fresh peppers showed higher volatile organic compounds content compared to dried ones, except for the two sweet peppers studied, which exhibited the opposite behavior. Our analysis underscores the variability in the effect of drying on volatile compound composition among different pepper species and even among different cultivars, highlighting key compounds that could facilitate species classification in dried powder. This research serves as a preliminary guide for promoting the utilization of various pepper species and cultivars as powder, enhancing product valorization. Full article
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<p>Morphological biodiversity of the chili cultivars studied. The selected <span class="html-italic">Capsicum</span> germplasm showed different phenotypes for fruit: morphology, color, weight, and pungency [<a href="#B4-plants-13-01131" class="html-bibr">4</a>]. The cultivars in the first row are <span class="html-italic">C. annuum</span> genotypes, the second row contains <span class="html-italic">C. baccatum</span> and <span class="html-italic">chacoense</span>, and the third and fourth rows contain <span class="html-italic">C. chinense</span> accessions. Within the larger cells, the genotypes with the lowest capsaicin levels are identified.</p>
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<p>Example PTR-TOFMS mass spectrum detected on fresh and dry sample for (<b>A</b>,<b>A1</b>) <span class="html-italic">C. annuum</span> (“Bell Pepper”) and (<b>B</b>,<b>B1</b>) <span class="html-italic">C. chinense</span> (“Fatalii”). Absent signals are highlighted with a red “x” at the bottom of each box.</p>
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<p>Representation of fresh and dried pepper samples on the first two axes of the PLSDA model composed of nine LVs.</p>
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<p>Representation of dried pepper samples on the first two axes of the PLSDA model composed of nine LVs.</p>
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26 pages, 43920 KiB  
Article
Herbal Spices as Food and Medicine: Microscopic Authentication of Commercial Herbal Spices
by Amjad Khan, Mushtaq Ahmad, Amir Sultan, Raees Khan, Jamil Raza, Sheikh Zain Ul Abidin, Siraj Khan, Muhammad Zafar, Mohammad N. Uddin and Mohsin Kazi
Plants 2024, 13(8), 1067; https://doi.org/10.3390/plants13081067 - 10 Apr 2024
Viewed by 1868
Abstract
Herbal spices are an agricultural commodity, economically very important and beneficial in primary healthcare in the food and medicine sectors. Herbal spices are used as food flavoring agents as well as in phytotherapies throughout the world and have nutritive benefits. The food and [...] Read more.
Herbal spices are an agricultural commodity, economically very important and beneficial in primary healthcare in the food and medicine sectors. Herbal spices are used as food flavoring agents as well as in phytotherapies throughout the world and have nutritive benefits. The food and medicine industries widely employ artificial or natural adulteration to retard the deterioration and utilization of these adulterants in food and medicine products has given rise to significant apprehension among consumers, primarily stemming from the potential health risks that they pose. Thus, their characterization for the purpose of identification, origin, and quality assurance is mandatory for safe human consumption. Here, we studied 22 samples of commonly traded herbal spices that belong to 20 different genera and 21 species comprising 14 families, investigated macroscopically or organoleptically as well as histologically under microscopic examination. In this study, we provide details on organoleptic features including appearance, taste, odor, color, shape, size, fractures, types of trichomes, and the presence of lenticels among the examined herbal spices and these features have great significance in the detection of both natural as well as artificial deterioration. In terms of microscopic characterization, each examined plant part comprising different anatomical characteristics has taxonomic importance and also provides useful information for authentication from natural adulterants. Furthermore, the studied taxa were also described with nutritive and therapeutic properties. For condiments, herbal beverages and medicinal purposes, different herbal parts such as leaves, floral buds, seeds, fruit, and accessory parts like mericarp, rhizome, bulbs, and bark were used and commercially traded. Similarly, in this study, the leaves of Cinnamomum tamala and Mentha spicata, the floral buds of Syzygium aromaticum, the seeds of Amomum subulatum, Brassica nigra, Punica granatum, Myristica fragrans, Phyllanthus emblica, and Elettaria cardamomum, the mericarp of Coriandrum sativum, and Cuminum cyminum were observed. As a result, we show the potential of herbal spices as a source of many valuable phytochemicals and essential nutrients for food, nutraceutical, and homoeopathic medicine. Full article
(This article belongs to the Section Phytochemistry)
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<p>Map showing sampling sites in the investigated area.</p>
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<p>(<b>a</b>) Herbal spices, (<b>b</b>) macroscopic examination, (<b>c</b>) sample preparation, (<b>d</b>) slide preparation, (<b>e</b>) microscopic examination, and (<b>f</b>) histology.</p>
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<p>(<b>A</b>) Micrograph of garlic bulb. (<b>B</b>) Transverse section of <span class="html-italic">Allium sativum</span> bulb: Cu—cuticle, Ep—epidermis, Co—cortex, En—endodermis, Vb—vascular bundles and Mt—mesophyll tissues. (<b>C</b>) Micrograph of greater cardamom. (<b>D</b>) Transverse section of <span class="html-italic">Amomum subulatum</span> fruit: Te—Testa, Sc—sclerenchymatous region, Og—oil globules and En—endospermic region.</p>
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<p>(<b>A</b>) Micrograph of mustard seed. (<b>B</b>) Transverse section of <span class="html-italic">Brassica nigra</span> seed: Te—testa, En—endosperm, Co—cotyledon, Em—embryo and Cen—chalazal endosperm. (<b>C</b>) Micrograph of pepper seed. (<b>D</b>) Transverse section of <span class="html-italic">Capsicum annuum</span> Seed: Sc—seed coat, Rg—ridges, Co—separately localized cotyledons, En—endosperm and Em—embryo.</p>
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<p>(<b>A</b>) Micrograph of malabar leaf. (<b>B</b>) Transverse section of <span class="html-italic">Cinnamomum tamala</span> leaf (<b>a</b>) leaf lamina, (<b>b</b>) leaf base and (<b>c</b>) leaf petiole: (<b>b</b>,<b>c</b>) Cu—cuticle, Ep—epidermis, Mc—mucilage cells, Vc—volatile oil cavity, Lp—lignified parenchyma, Pp—pitted parenchyma, Sc—sclerenchyma, Co—collenchyma, Pc—pericycle, Mc—mesophyll cell, Pc—palisade, Xy—xylem, Mc—mucilage cavity, Ph—phloem, Stc—stone cell with tannin and Sc—sclerenchyma. (<b>C</b>) Micrograph of cinnamon bark. (<b>D</b>) Transverse section of <span class="html-italic">Cinnamomum verum</span> bark: Ct—cork tissues, Pf—pericyclic fibers, Sc—sclereids, Sp—secondary phloem, Mr—medullary ray, Mc—mucilage cells, Pf—phloem fibers and Oc—oil cavity.</p>
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<p>(<b>A</b>) Micrograph of coriander mericarp. (<b>B</b>) Transverse section of <span class="html-italic">Coriandrum sativum</span> mericarp: Rd-ridges, Ep—epicarp, Me—mesocarp, Ec—endocarp, Cp—carpophore, Ts—testa and En—endosperm. (<b>C</b>) Micrograph of cumin mericarp. (<b>D</b>) Transverse section of <span class="html-italic">Cuminum cyminum</span> mericarp: Rd—ridges, Ep—epicarp, Vt—vittae, Me—mesocarp, Ec—endocarp, Ts—testa, Cp—carpophore and En—endosperm.</p>
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<p>(<b>A</b>) Micrograph of turmeric rhizome. (<b>B</b>) Transverse section of <span class="html-italic">Curcuma longa</span> rhizome: (<b>a</b>) Fb—fibers and Vs—vessels. (<b>b</b>) Oc.c—outer cork cells, Pt—parenchymatous tissue, Ic.c—inner cork cells, Sg—starch grains and Oc—oleoresin cells. (<b>C</b>) Micrograph of cardamom fruit. (<b>D</b>) Transverse section of <span class="html-italic">Elettaria cardamomum</span> fruit: (<b>c</b>) Ep—epidermis, Lu—lumen and Oc—oil cellular layer (<b>d</b>) Sc—sclerenchymatous layer, En—endosperm, Rh—raphe and Ps—palisade sclerenchyma.</p>
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<p>(<b>A</b>) Micrograph of fennel mericarp. (<b>B</b>) Transverse section of <span class="html-italic">Foeniculum vulgare</span> mericarp: Rd—ridges, Ep—epicarp, Vt—vittae, Rp—raphe, Me—mesocarp, Ec—endocarp, Vb—vascular bundle and En—endosperm. (<b>C</b>) Micrograph of star anise fruit. (<b>D</b>) Transverse section of <span class="html-italic">Illicium verum</span> fruit: (<b>a</b>) Ep—epicarp, Gt—ground tissue, Ec—endocarp region, and Co—cotyledon. (<b>b</b>) Ts—testa, Oc—oil cavity Lu—lumen and Me—mesocarp region.</p>
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<p>(<b>A</b>) Micrograph of mace seed endosperm. (<b>B</b>) Transverse section of <span class="html-italic">Myristica fragrans</span> seed endosperm: Sp—spongy parenchyma, Dp—dilated parenchyma, Oc—oil cavity, En—endosperm and Pig—perisperm inner growth. (<b>C</b>) Micrograph of fruit peel of Mace. (<b>D</b>) Cross-section of fruit peel of <span class="html-italic">Myristica fragrans</span>: Vb—vascular bundle, Gp—ground parenchyma, Oc—oil cavity, and Vo—volatile oil contents.</p>
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<p>(<b>A</b>) Micrograph of spearmint leaf and young twig. (<b>B</b>) Transverse section of <span class="html-italic">Mentha spicata</span> leaf and young twig: (<b>a</b>) Ep—epidermis, Cgt—capitate glandular trichomes, Co—collenchyma cells, Pt—palisade tissues, Vb—vascular bundle, Pc—pericycle and Sp—spongy parenchyma. (<b>b</b>) Ep—epidermis, Cor—collenchymatous ridges, Co—cortex, Pc—pericycle, Ph—phloem, Xy—xylem, Tr—trichomes and Pi—pith. (<b>C</b>) Micrograph of emblic fruit. (<b>D</b>) Transverse section of <span class="html-italic">Phyllanthus emblica</span> fruit: Gt—ground tissue, C.o.c—calcium oxalate crystals, Sp—spongy parenchyma, En—endosperm, Ec—endocarp region and Vb—vascular bundle.</p>
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<p>(<b>A</b>) Micrograph of black pepper fruit. (<b>B</b>) Transverse section of <span class="html-italic">Piper nigrum</span> fruit: Pc—pericap, Ps—perisperm, Rd—ridges, Og—oil globules, and En—endosperm. (<b>C</b>) Micrograph of Pomegranate seed. (<b>D</b>) Transverse section of <span class="html-italic">Punica granatum</span> seed: (<b>a</b>) Sc—seed coat, En—endosperm and Em—embryo. (<b>b</b>) P.La—pigmented layer and C.co—coiled cotyledons.</p>
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<p>(<b>A</b>) Micrograph of Clove flower bud. (<b>B</b>) Transverse section of <span class="html-italic">Syzygium aromaticum</span> flower bud: CU—cuticle, EP—epidermis, OG—oil glands, COL—columella, SPH—sphaeraphide, VB—vascular bundles and AER—aerenchyma. (<b>C</b>) Micrograph of Carom Mericarp. (<b>D</b>) Transverse section of <span class="html-italic">Trachyspermum ammi</span> mericarp: Rd—ridges, Ep—epicarp, VT—vittae, SC—secretory cells, ME—mesocarp, EC—endocarp, CP—carpophore and EN—endosperm.</p>
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<p>(<b>A</b>) Micrograph of fenugreek seed. (<b>B</b>) Transverse section of <span class="html-italic">Trigonella foenum-graecum</span> seed: Ts—testa, En—endosperm, Cen—chalazal endosperm, Em—embryo and Co—both the cotyledon. (<b>C</b>) Micrograph of ginger rhizome. (<b>D</b>) Transverse section of <span class="html-italic">Zingiber officinale</span> rhizome: (<b>a</b>,<b>b</b>) Oc—outer cork Co—cortex and Gt—ground tissues (<b>b</b>) Oc—outer cork, Ic—inner cork, Co—cortex, Sg—starch grains, Fvb—fibro—vascular bundles, Fb—fibers and Oc—oleoresin cells.</p>
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<p>Representative plant families (numbers of taxa and its percentage).</p>
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<p>Representative plant parts traded (including frequency and its percentage).</p>
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15 pages, 3026 KiB  
Article
A Study of Resistome in Mexican Chili Powder as a Public Health Risk Factor
by Mayra Paola Mena Navarro, Merle Ariadna Espinosa Bernal, Claudia Alvarado Osuna, Miguel Ángel Ramos López, Aldo Amaro Reyes, Jackeline Lizzeta Arvizu Gómez, Juan Ramiro Pacheco Aguilar, Carlos Saldaña Gutiérrez, Victor Pérez Moreno, José Alberto Rodríguez Morales, María Carlota García Gutiérrez, Erika Álvarez Hidalgo, Jorge Nuñez Ramírez, José Luis Hernández Flores and Juan Campos Guillén
Antibiotics 2024, 13(2), 182; https://doi.org/10.3390/antibiotics13020182 - 13 Feb 2024
Viewed by 1991
Abstract
Chili powder is an important condiment around the world. However, according to various reports, the presence of pathogenic microorganisms could present a public health risk factor during its consumption. Therefore, microbiological quality assessment is required to understand key microbial functional traits, such as [...] Read more.
Chili powder is an important condiment around the world. However, according to various reports, the presence of pathogenic microorganisms could present a public health risk factor during its consumption. Therefore, microbiological quality assessment is required to understand key microbial functional traits, such as antibiotic resistance genes (ARGs). In this study, metagenomic next-generation sequencing (mNGS) and bioinformatics analysis were used to characterize the comprehensive profiles of the bacterial community and antibiotic resistance genes (ARGs) in 15 chili powder samples from different regions of Mexico. The initial bacterial load showed aerobic mesophilic bacteria (AMB) ranging between 6 × 103 and 7 × 108 CFU/g, sporulated mesophilic bacteria (SMB) from 4.3 × 103 to 2 × 109 CFU/g, and enterobacteria (En) from <100 to 2.3 × 106 CFU/g. The most representative families in the samples were Bacillaceae and Enterobacteriaceae, in which 18 potential pathogen-associated species were detected. In total, the resistome profile in the chili powder contained 68 unique genes, which conferred antibiotic resistance distributed in 13 different classes. Among the main classes of antibiotic resistance genes with a high abundance in almost all the samples were those related to multidrug, tetracycline, beta-lactam, aminoglycoside, and phenicol resistance. Our findings reveal the utility of mNGS in elucidating microbiological quality in chili powder to reduce the public health risks and the spread of potential pathogens with antibiotic resistance mechanisms. Full article
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<p>Taxonomic composition in fifteen chili powder samples. The bar graph represents the relative abundance of representative bacterial families as a percentage, with values being indicated by different colors.</p>
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<p>Heatmap of bacterial taxonomic composition in chili powder samples. Significant bacterial species composition was determined from the unique reads per million (rPM) that were mapped to specific microbial taxa using CZ-ID platform indicated by a colored logarithmic scale, with darker red representing the highest rPM.</p>
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<p>Alpha and beta diversity plots to visualize the difference of bacterial composition in chili powder samples. (<b>A</b>) Alpha diversity comparisons based on Shannon diversity index and (<b>B</b>) PCoA plot of beta diversity measure with the Bray–Curtis dissimilarity.</p>
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<p>Heatmap of pathogen-related bacteria distribution in chili powder samples. Significant bacterial taxa composition was determined from the unique reads per million (rPM) using CZ-ID platform indicated by a colored logarithmic scale, with darker red representing the highest rPM.</p>
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<p>Relative abundance of antibiotic resistance classes. In total, 13 antibiotic resistance classes are indicated by graphic in 15 chili powder samples.</p>
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<p>Heatmap of ARG distribution between chili powder samples. Results were obtained in CZ-ID platform and indicated by a colored scale, with darker red representing the highest readings.</p>
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3 pages, 178 KiB  
Editorial
Harvesting Knowledge: Illuminating Advances in Brassica Crops Genomics and Breeding
by Xu Cai, Jian Wu and Xiaowu Wang
Horticulturae 2023, 9(12), 1332; https://doi.org/10.3390/horticulturae9121332 - 12 Dec 2023
Cited by 1 | Viewed by 1251
Abstract
Brassica crops encompass a diverse array, including vegetables, oil crops, ornamentals, and condiments [...] Full article
(This article belongs to the Special Issue Advances in Brassica Crops Genomics and Breeding)
18 pages, 5097 KiB  
Review
Nutritional Values and Bio-Functional Properties of Fungal Proteins: Applications in Foods as a Sustainable Source
by Ku Li, Kaina Qiao, Jian Xiong, Hui Guo and Yuyu Zhang
Foods 2023, 12(24), 4388; https://doi.org/10.3390/foods12244388 - 6 Dec 2023
Cited by 3 | Viewed by 2952
Abstract
From the preparation of bread, cheese, beer, and condiments to vegetarian meat products, fungi play a leading role in the food fermentation industry. With the shortage of global protein resources and the decrease in cultivated land, fungal protein has received much attention for [...] Read more.
From the preparation of bread, cheese, beer, and condiments to vegetarian meat products, fungi play a leading role in the food fermentation industry. With the shortage of global protein resources and the decrease in cultivated land, fungal protein has received much attention for its sustainability. Fungi are high in protein, rich in amino acids, low in fat, and almost cholesterol-free. These properties mean they could be used as a promising supplement for animal and plant proteins. The selection of strains and the fermentation process dominate the flavor and quality of fungal-protein-based products. In terms of function, fungal proteins exhibit better digestive properties, can regulate blood lipid and cholesterol levels, improve immunity, and promote gut health. However, consumer acceptance of fungal proteins is low due to their flavor and safety. Thus, this review puts forward prospects in terms of these issues. Full article
(This article belongs to the Section Food Security and Sustainability)
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<p>Network visualization of the titles and abstracts in the selected 10,000 studies related to topics regarding the nutrition and function of fungal proteins from 2000 to 2024.</p>
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<p>The production process of fungal proteins.</p>
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<p>Health benefits of fungal proteins.</p>
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15 pages, 2626 KiB  
Article
Piperine Extraction and Encapsulation in Polycaprolactone Nanoparticles
by Julia Cedran Coco, Luiza Aparecida Luna Silvério, Érica Mendes dos Santos, Ana Claudia Sueiro, Janaína Artem Ataide, Ana Cláudia Paiva-Santos and Priscila Gava Mazzola
Cosmetics 2023, 10(6), 152; https://doi.org/10.3390/cosmetics10060152 - 6 Nov 2023
Cited by 1 | Viewed by 2602
Abstract
Black pepper, a commonly utilized culinary condiment, holds significant importance in Ayurvedic and Chinese medicinal practices due to its various biological benefits, including antioxidant, antimicrobial, and anti-inflammatory properties. To amplify these attributes and enhance their efficacy in formulations, the utilization of nanocarriers presents [...] Read more.
Black pepper, a commonly utilized culinary condiment, holds significant importance in Ayurvedic and Chinese medicinal practices due to its various biological benefits, including antioxidant, antimicrobial, and anti-inflammatory properties. To amplify these attributes and enhance their efficacy in formulations, the utilization of nanocarriers presents a promising approach. Thus, the objective of this study was to obtain a pepper extract, evaluate its constituents, and encapsulate it in polymeric nanoparticles. The ethanol extract of the grains powder had a higher concentration of piperine and better antioxidant activity when compared to whole grains. Pepper extract encapsulation efficiency in terms of piperine concentration was 84.8 ± 3.5%, and a sustained and prolonged release profile was observed, as well as other studies in the literature using polycaprolactone (PCL). The presence of the extract did not change the instability index and the sedimentation velocity of the nanoparticles, as well as the polydispersity index and the zeta potential of nanoparticles. However, there was a difference in the mean size and concentration of particles. This study highlights the potential of PCL nanoparticles as a promising delivery system for black pepper extract, which could have various applications in the cosmetic and pharmaceutical industries, maximizing the benefits of black pepper extract. Full article
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<p>Antioxidant activity in DPPH assay for grain, powder pepper extracts, and extract-containing nanoparticles (NP-PCL-Extract).</p>
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<p>Representative cryo-TEM images of empty PCL nanoparticles (<b>a</b>) and extract-loaded nanoparticles (<b>b</b>) from an aqueous suspension.</p>
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<p>Cumulative piperine in vitro released (%) from piperine standard, pepper extract, and extract-containing nanoparticles (NP-PCL-Extract) throughout pre-determined time intervals (<b>a</b>) and in detail of the first 8 h (<b>b</b>).</p>
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<p>Piperine flux during piperine release assay from piperine standard, pepper extract, and extract-containing nanoparticles (NP-PCL-Extract). The * symbol indicates significant statistical difference between samples (one-way ANOVA with Tukey posttest, <span class="html-italic">p</span> &lt; 0.05).</p>
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<p>Recorded evolution of transmission profiles of empty (<b>a</b>) and extract-loaded nanoparticles (<b>b</b>). The abscissa shows the cell position, and the ordinate represents the percentage of light transmission. The initial profiles are indicated by red lines, while the most recent profiles are represented by green lines over time.</p>
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<p>Size and polydispersity index measured by dynamic light scattering of (<b>a</b>) unloaded PCL nanoparticles (NP-PCL) and (<b>b</b>) loaded with black pepper extract (NP-PCL-Extract) over 180 days. The * symbol indicates significant statistical difference when compared to first day of production (one-way ANOVA followed by Dunnett’s multiple comparison test, <span class="html-italic">p</span> &lt; 0.05).</p>
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