Search Results (36)

Broomcorn millet (Panicum miliaceum L.) is one of the earliest crops, domesticated nearly 8000 years ago in northern China. It gradually spread across the entire Eurasian continent, as well as to America and Africa, with recent improvement in various reproductive and vegetative traits. To identify the genes that were selected during the domestication and improvement processes, we performed a comparative transcriptome analysis based on wild types, landraces, and improved cultivars of broomcorn millet at both seeding and filling stages. The variations in gene expression patterns between wild types and landraces and between landraces and improved cultivars were further evaluated to explore the molecular mechanisms underlying the domestication and improvement of broomcorn millet. A total of 2155 and 3033 candidate genes involved in domestication and a total of 84 and 180 candidate genes related to improvement were identified at seedling and filling stages of broomcorn millet, respectively. The annotation results suggested that the genes related to metabolites, stress resistance, and plant hormones were widely selected during both domestication and improvement processes, while some genes were exclusively selected in either domestication or improvement stages, with higher selection pressure detected in the domestication process. Furthermore, some domestication- and improvement-related genes involved in stress resistance either lost their functions or reduced their expression levels due to the trade-offs between stress resistance and productivity. This study provided novel genetic materials for further molecular breeding of broomcorn millet varieties with improved agronomic traits. Full article

Broomcorn millet (Panicum miliaceum L.) is a crop with a good ability to adapt to the environment. Over 8800 accessions have been collected in the national gene bank of China. The huge quantity of germplasms made it difficult for analysis and evaluation. Although a broomcorn millet core collection (CC) comprising 780 accessions was established, the number is still too large for researchers to explore in depth. In this study, the genetic diversity of 634 broomcorn millet accessions from the core collection was analyzed based on SSR markers. A mini-core collection (MC) containing 256 accessions was extracted. The mini-core collection accounted for less than half of the original core collection and only about 2.8% of the total resources but still provided a good representation. In addition, the results of this study validated that Shanxi is the origin of broomcorn millet, and accessions from the South region may contain novel genes. In conclusion, this study provides a comprehensive characterization of the genetic diversities of broomcorn millet core collection in China. Moreover, an MC may aid in reasonably and efficiently selecting materials for broomcorn millet breeding as researchers could screen for aimed genetic characters within a smaller scope. Full article

Broomcorn millet (Panicum miliaceum) is among the earliest domesticated staple crops in the world’s agricultural history and facilitated the development of several early agrarian cultures, particularly those originating in northern China. However, the propagation route of broomcorn millet in China from the Middle Ages to the present remains unclear. The aim of this study is to explore the genetic affinity between ancient and modern millet samples, trace the genetic origins and diffusion pathways of broomcorn millet, and provide insights into its domestication and spread. To achieve this, we sequence ancient DNA from broomcorn millet remains excavated from the Chashan Village cemetery (AD 691) in Gansu Province, China. Phylogenetic and population genetic analyses, integrating ancient and modern millet genomes, reveal a close genetic relationship between ancient millet and contemporary millet from Ningxia Province (445 km away from Chashan Village), suggesting a potential origin for the Chashan millet. This finding aligns with the tomb’s epitaph, which documents the reburial of the tomb’s owner, who was originally buried in Ningxia, and provides important archaeological evidence for understanding the interaction between geopolitical dynamics and the natural environment in northwest China during the late seventh century. Furthermore, outgroup-f3 and D statistics evidence suggests substantial genetic interactions between ancient millet and modern varieties from the Loess Plateau, Huang-Huai-Hai Plain, and Northeast Plain, indicating the dispersal route of broomcorn millet, along with human migration routes, from the northwest to northern China and ultimately to the northeast region, starting from the Middle Ages onward. This study enhances our understanding of millet’s genetic history, offers a novel perspective on burial archaeology, and provides valuable insights into the origins, domestication, and diffusion of broomcorn millet. Full article

This study aimed to elucidate responses of the bacterial structure and diversity of the rhizosphere in flowering broomcorn millet after rehydration following drought stress. In this study, the broomcorn millet varieties ‘Hequ red millet’ (A1) and ‘Yanshu No.10′ (A2), known for their different drought tolerance levels, were selected as experimental materials. The plants were subjected to rehydration after drought stress at the flowering stage, while normal watering (A1CK and A2CK) served as the control. Soil samples were collected at 10 days (A11, A21, A1CK1, and A2CK1) and 20 days (A12, A22, A1CK2, and A2CK2) after rehydration. High-throughput sequencing technology was employed to investigate the variations in bacterial community structure, diversity, and metabolic functions in the rhizosphere of the broomcorn millet at different time points following rehydration. The findings indicated that the operational taxonomic units (OTUs) of bacteria in the rhizosphere of broomcorn millet were notably influenced by the duration of treatment, with a significant decrease in OTUs observed after 20 days of rehydration. However, bacterial Alpha diversity was not significantly impacted by rehydration following drought stress. The bacterial community in the rhizosphere of broomcorn millet was mainly composed of Actinobacteria and Proteobacteria. After rewatering for 10 to 20 days after drought stress, the abundance of Sphingomonas and Aeromicrobium in the rhizosphere soil of the two varieties of broomcorn millet decreased gradually. Compared with Yanshu No.10, the abundance of Pseudarthrobacter in the rhizosphere of Hequ red millet gradually increased. A Beta diversity analysis revealed variations in the dissimilarities of the bacterial community which corresponded to different rehydration durations. The relative abundance of bacterial metabolic functions in the rhizosphere of broomcorn millet was lower after 20 days of rehydration, compared to measurements after 10 days of rehydration. This observation might be attributed to the exchange of materials between broomcorn millet and microorganisms during the initial rehydration stage to repair the effects of drought, as well as to the enrichment of numerous microorganisms to sustain the stability of the community structure. This study helps to comprehend the alterations to the bacterial structure and diversity in the rhizosphere of broomcorn millet following drought stress and rehydration. It sheds light on the growth status of broomcorn millet and its rhizosphere microorganisms under real environmental influences, thereby enhancing research on the drought tolerance mechanisms of broomcorn millet. Full article

China has a 9000-year-long history of cereal-based alcohol production, with the use of molds (filamentous fungi) likely being one of the earliest fermentation techniques. This method later developed into the uniquely East Asian qu (koji) starter compound, containing grains, molds, yeasts, and bacteria. Recent studies have revealed that this method was already widely applied during the Neolithic period. However, much less is known about its development during the early dynastic times, and our knowledge of this innovation has mainly relied on textual materials. Here, we present direct evidence, based on microbotanical, microbial, and chemical analyses, for the fermentation method of a 2300-year-old liquid preserved in a sealed bronze bottle unearthed in a Qin tomb at Yancun, Shaanxi. The results of this research suggest that this liquid is likely a fermented beverage made from wheat/barley, rice, Job’s tears, broomcorn millet, and pulses. The fermentation starter may have been a cereal-based qu, consisting of a wide range of microorganisms, including molds (Aspergillus and Monascus), yeasts, and bacteria. Our findings suggest that the tradition of selecting suitable grains and microbial communities for brewing alcohol, possibly with a maiqu starter (primarily wheat/barley-based qu), may have been well established more than two thousand years ago. Full article

Exploring crop yield stability and the relationship between the water–fertilizer effect and annual precipitation type in a broomcorn millet–potato–spring corn rotation system under long-term fertilization on chestnut cinnamon soil in loess tableland can provide a scientific basis for rational fertilization in the northwest Shanxi region in years with different precipitation. This study was based on a 33-year long-term fertilizer experiment, using four fertilizer treatments: no fertilizer as control (CT), single fertilizer nitrogen (N), single organic fertilizer (M), and nitrogen fertilizer with organic fertilizer (NM). The results showed that broomcorn millet and maize had the highest yield in wet years, while potatoes had the highest yield in normal years and the yield under NM treatment was the highest. The sustainable yield index (SYI) values for potato and maize were higher than the SYI for the broomcorn millet during years with different precipitation and the SYI for the NM treatment was the highest. The water use efficiency of NM treatment was the highest. The yield of broomcorn millet and maize was affected by nitrogen fertilizer, organic fertilizer, and precipitation during the growth period, while the potato yield was mainly affected by nitrogen fertilizer and organic fertilizer. Therefore, the rotation of potato–maize and the rational allocation of organic and inorganic fertilizer (NM) is the best planting system in this region. Full article

The spatio-temporal evolution of human subsistence strategies and their driving force in prehistoric Eurasia has received increasing attention with the rapid development of archaeobotanical, zooarchaeological, and isotopic research in recent decades, while studies focusing on the historical periods are relatively absent. In the Hexi Corridor in northwestern China, which has served as a hub for trans-Eurasian exchange since the late prehistoric period, archaeobotanical data have been reported from numerous Neolithic and Bronze Age sites, as well as sites from the Wei and Jin Dynasties (220–420 BCE) to the Yuan Dynasty (1271–1368 BCE). However, no archaeobotanical study has been conducted at sites of the Han Dynasty (202 BCE–220 CE), a crucial period connecting prehistoric and historical eras. In this study, we identified 32475 plant remains, including 31,463 broomcorn millets, 233 foxtail millets, and 780 weeds, from the Shuangdun North Beacon Tower (SDNBT) site of the Han Dynasty at the western end of the Hexi Corridor, suggesting that millets played a prominent part in human subsistence strategies in the area during this period. In addition, sheep, chicken, dog, horse, and rodent remains were also collected at the site. By applying a multi-disciplinary approach, we detected a remarkable change in plant-based subsistence in the ancient Hexi Corridor. Specifically, the importance of millet crops, compared with other crops (especially barley and wheat), in plant-based subsistence declined from the Late Neolithic to the Bronze Age; it apparently improved during the Han and Sui-Tang Dynasties (581–907 CE), when agricultural empires controlled the area, and then declined again during the Wei, Jin, Northern, and Southern Dynasties (220–581 CE) and the Song-Yuan Dynasty (960–1368 CE), when nomadic regimes controlled the area. Climate change, trans-Eurasian exchanges, and geopolitical shifts influenced the diachronic change in plant-based subsistence from the Late Neolithic to the historical periods in the Hexi Corridor. Full article

The flowering stage is a critical period for water sensitivity and quality formation of broomcorn millets. However, the effects and mechanisms of drought stress on the quality formation of broomcorn millets are not clear. We used the drought-resistant variety Hequ red millet (H) and the drought-sensitive variety Yanshu No. 10 (Y) were used as materials for drought stress treatment during the flowering stage, metabolomics and physiological methods were used to study the differences in protein, starch, amino acids, medium and medium-long chain fatty acids, and their response characteristics to drought in broomcorn millet. The results showed that different genotypes of broomcorn millets exhibited different response mechanisms in the face of drought stress. In Hequ red millet, drought stress significantly increased the contents of amylopectin (2.57%), pyridoxine (31.89%), and anthocyanin, and significantly decreased the contents of water-soluble protein (5.82%), glutelin (10.07%), thiamine (14.95%) and nicotinamide (23.01%). In Yanshu No. 10, drought significantly decreased amylose by 6.05%, and significantly increased riboflavin and nicotinamide contents by 21.11% and 32.59%. Correlation analysis showed that total starch and amylose were highly significantly positively correlated with methyl palmitate; negatively correlated with amylopectin, vitamins, proteins, free amino acids, and medium-long chain fatty acids; and amylopectin was significantly positively correlated with water-soluble protein, riboflavin, and pyridoxine. Water-soluble protein and glutelin were significantly positively correlated with most free amino acids, medium-long chain fatty acids, and nicotinamide. Thiamine showed significant positive correlation with nicotinamide and significant negative correlation with pyridoxine. Riboflavin was significantly positively correlated with nicotinamide, pyridoxine, and water-soluble protein, and pyridoxine was significantly positively correlated with water-soluble protein. Hequ red millet transforms into amylopectin by consuming water-soluble protein and glutelin, and improves drought resistance by accumulating pyridoxine, and changes its physicochemical properties by decreasing the content of amylose and protein and elevating the content of amylopectin. Yanshu No. 10 resisted drought by catabolizing lipids to produce fatty acids and by consuming amylose for conversion into other metabolites. The present study helps to understand the response of the nutritional quality of millets to drought stress at the flowering stage and provides a theoretical basis for the selection and breeding of superior varieties of millets and drought resistance research. Full article

Drought stress restricts plant growth and development. The flowering stage is a period of abundant microbial diversity in the rhizosphere of broomcorn millet. However, the diversity and community structure of rhizosphere fungi during the flowering stage of broomcorn millet and the response mechanism to drought stress are still unclear. In this study, two broomcorn millet varieties, ‘Hequ red millet’ (A1) and ‘Yanshu No.10′ (A2), with different drought resistances, were used as experimental materials. Using the pot water control method, drought treatment at the flowering stage was carried out, and normal watering (A1CK, A2CK) was used as the control. High-throughput sequencing technology was used to study the diversity and structural changes in rhizosphere fungi in broomcorn millet. The results show that the number of fungi OTUs in the A1, A2, A1CK and A2CK samples were 445, 481, 467 and 434, respectively, of which 331 OTUs were shared by all groups. The fungal community in the rhizosphere of broomcorn millet was mainly composed of Ascomycota and Basidiomycota. Drought treatment significantly reduced the abundance of Mortierella and significantly increased the abundance of Phoma. The abundance of Nectriaceae in the rhizosphere soil of ‘Hequ Red millet’ was significantly increased. The abundance of Pseudocercospora in the rhizosphere soil of ‘Yanshu No.10′ was higher, and the lower was Hypocreales and Nectriaceae. However, there was no significant difference in the alpha diversity of fungal communities in the four treatments, and the fungal community structure between A2 and A1CK was more similar, whereas the difference between A1 and A2CK was larger. Correlation analysis showed that drought stress had little effect on the interaction of rhizosphere fungi, and metabolic functions such as nucleotide metabolism and electron transport in rhizosphere fungi accounted for a relatively high proportion. The results show that the diversity and community structure of rhizosphere fungi were less affected by drought, which may have been due to the close interaction between species, which made the fungal community more stable under drought stress, and the difference in planting varieties may have affected the enriched rhizosphere fungal species. Full article

The eastern Baltic region represents the world’s most northerly limit of successful broomcorn millet (Panicum miliaceum) (hereafter, millet) cultivation in the past, yet this crop has been almost forgotten today. The earliest millet in the eastern Baltic region has been identified from macrobotanical remains which were directly dated to ca 1000 BCE. Between 800 and 500 BCE, millet was one of the major staple foods in the territory of modern-day Lithuania. Millet continued to play an important role in past agriculture up until the 15th century, with its use significantly declining during the following centuries. This paper analyses both the archaeobotanical records and written sources on broomcorn millet cultivation in Lithuania from its first arrival all the way through to the 19th century. The manuscript reviews the evidence of millet cultivation in the past as documented by archaeobotanical remains and historical accounts. In light of fluctuating records of millet cultivation through time, we present the hypothetical reasons for the decline in millet use as human food. The paper hypothesizes that the significant decrease in broomcorn millet cultivation in Lithuania from the 15th century onwards was likely influenced by several factors, which include climate change (the Little Ice Age) and the agricultural reforms of the 16th century. However, more detailed research is required to link past fluctuations in millet cultivation with climatic and historical sources, thus better understanding the roots of collapsing crop biodiversity in the past. Full article

The overuse of nitrogen fertilizers has led to environmental pollution, which has prompted the widespread adoption of biochar as a soil conditioner in agricultural production. To date, there has been a lack of research on the effects of biochar and its combination with nitrogen fertilizer on the quality of broomcorn millet (Panicum miliaceum L.) starch. Thus, this study examined the physicochemical characteristics of starch in two types of broomcorn millet (waxy and non-waxy) under four different conditions, including a control group (N0), nitrogen fertilizer treatment alone (N150), biochar treatment alone (N0+B), and a combination of biochar and nitrogen fertilizer treatments (N150+B). The results showed that, in comparison to the control, all the treatments, particularly N150+B, decreased the content of amylose and gelatinization temperature and enhanced the starch transparency gel consistency and swelling power. In addition, biochar can improve the water solubility of starch and the gelatinization enthalpy. Importantly, the combination of biochar and nitrogen fertilizer increased the proportion of A-granules, final viscosity, starch content, and the average degree of amylopectin in polymerization. Thus, this research indicates that the combinations of biochar and nitrogen fertilizer result in the most significant improvement in the quality of starch produced from broomcorn millet. Full article

In this paper, we use preliminary archaeological data spanning the Iron Age through Medieval periods (ca. 800 BCE to 1200 CE) in the Juuku Valley in Kyrgyzstan on the south side of Lake Issyk-Kul to model land use across vertical mountain zones. We have (1) established a radiometric chronology; (2) conducted test excavations of an Iron Age settlement at 2100 m asl and a Turkic period burial at 1934 m asl; (3) undertaken preliminary archaeobotanical research; and (4) performed pedestrian surveys. Archaeobotanical remains of wheat (Triticum aestivum), barley (Hordeum vulgare), broomcorn millet (Panicum milaceum), foxtail millet (Setaria italica), and legumes were recovered in very small quantities from both sites. We compare these preliminary archaeobotanical results with previously published data from Talgar Iron Age settlements on the north side of the Tian Shan Mountain range in Kazakhstan. A small assemblage of faunal remains found at the Turkic period kurgan and from a profile at the upland Iron Age settlement demonstrates the practice of herding sheep/goats, cattle, and horses in the Juuku Valley. The goal of this study was to test the hypothesis that pastoral transhumance and agropastoralism were interchangeable economic strategies used by peoples in the Iron Age through Medieval periods in mountain-river valleys between 600 m to 2100 m asl. These economic strategies combined the pasturing of sheep, goats, cattle, and horses with the cultivation of cereals in a system that was adapted to different vegetational zones along a vertical gradient. This paper is based on preliminary research using survey data and test excavations and initiates a long-term research study of four millennia of settlements that appear to have ranged from pastoral transhumance and combined mountain agriculture. Full article

Broomcorn millet Huangjiu brewing is usually divided into primary fermentation and post-fermentation. Microbial succession is the major factor influencing the development of the typical Huangjiu flavor. Here, we report the changes in flavor substances and microbial community during the primary fermentation of broomcorn millet Huangjiu. Results indicated that a total of 161 volatile flavor compounds were measured during primary fermentation, and estragole was detected for the first time in broomcorn millet Huangjiu. A total of 82 bacteria genera were identified. Pediococcus, Pantoea, and Weissella were the dominant genera. Saccharomyces and Rhizopus were dominant among the 30 fungal genera. Correlation analysis showed that 102 microorganisms were involved in major flavor substance production during primary fermentation, Lactobacillus, Photobacterium, Hyphodontia, Aquicella, Erysipelothrix, Idiomarina, Paraphaeosphaeria, and Sulfuritalea were most associated with flavoring substances. Four bacteria, Lactobacillus (R1), Photobacterium (R2), Idiomarina (R3), and Pediococcus (R4), were isolated and identified from wheat Qu, which were added to wine Qu to prepare four kinds of fortified Qu (QR1, QR2, QR3, QR4). QR1 and QR2 fermentation can enhance the quality of Huangjiu. This work reveals the correlation between microorganisms and volatile flavor compounds and is beneficial for regulating the micro-ecosystem and flavor of the broomcorn millet Huangjiu. Full article

Xinjiang, in Northwestern China, was a key point in the prehistoric trans-Eurasian network of exchange and played an important role in facilitating the dispersal of crops across Eurasia. Millet crops were first cultivated and used ca. 10,000 years ago in Northern China, from where they spread via different routes, leaving intriguing traces in various sites across Xinjiang. This paper presents the latest data on millet in Xinjiang. By employing a multidisciplinary approach, including radiocarbon dating, archaeobotanical evidence, and carbon isotope datasets, this study explores potential routes by which millet entered Xinjiang and traces its expansion from the third millennium BC to the 10th century AD. The research highlights the significant role of millet in shaping the ancient economies and cultures of Xinjiang and Central Asia, while also underscoring the importance of further investigation to uncover the complex pathways of its dispersal across Eurasia. Full article

This paper proposes to improve the information provided by biological indicators from sedimentary archives by integrating biomolecular techniques and botanical skills. This study represents a first proposal for combining pollen and biomolecular markers to detect land use and improve knowledge of past environmental change drivers. The specific aim of the research is to verify the relationship between miliacin (a pentacyclic triterpene methyl ether, usually interpreted as a broomcorn millet biomarker) and Panicum pollen in three near-site stratigraphic sequences of the Terramara S. Rosa di Poviglio (Po Plain, N Italy). The three cores span the last ~15,000 years and potentially record the beginning of Panicum miliaceum cultivation attested in the area since at least the Bronze Age within the Terramare culture. Despite the fact that Panicum pollen grains were rare in the spectra and miliacin was barely detectable in most of the 31 samples selected for biomolecular analyses, their combined evidence testifies to the local presence of the plant. Panicum pollen and sedimentary miliacin suggest the adoption of millet crops during the Recent Bronze Age by the Terramare culture, when climatic instability led to the diversification of cereal crops and the shift to drought-tolerant varieties. Full article