Search Results (2,178)

GoodBiome™ Foods are functional foods containing a probiotic (Bacillus subtilis HU58™) and prebiotics (mainly inulin). Their effects on the human gut microbiota were assessed using ex vivo SIFR^® technology, which has been validated to provide clinically predictive insights. GoodBiome™ Foods (BBM/LCM/OSM) were subjected to oral, gastric, and small intestinal digestion/absorption, after which their impact on the gut microbiome of four adults was assessed (n = 3). All GoodBiome™ Foods boosted health-related SCFA acetate (+13.1/14.1/13.8 mM for BBM/LCM/OSM), propionate (particularly OSM; +7.4/7.5/8.9 mM for BBM/LCM/OSM) and butyrate (particularly BBM; +2.6/2.1/1.4 mM for BBM/LCM/OSM). This is related to the increase in Bifidobacterium species (B. catenulatum, B. adolescentis, B. pseudocatenulatum), Coprococcus catus and Bacteroidetes members (Bacteroides caccae, Phocaeicola dorei, P. massiliensis), likely mediated via inulin. Further, the potent propionogenic potential of OSM related to increased Bacteroidetes members known to ferment oats (s key ingredient of OSM), while the butyrogenic potential of BBM related to a specific increase in Anaerobutyricum hallii, a butyrate producer specialized in the fermentation of erythritol (key ingredient of BBM). In addition, OSM/BBM suppressed the pathogen Clostridioides difficile, potentially due to inclusion of HU58™ in GoodBiome™ Foods. Finally, all products enhanced a spectrum of metabolites well beyond SCFA, including vitamins (B3/B6), essential amino acids, and health-related metabolites such as indole-3-propionic acid. Overall, the addition of specific ingredients to complex foods was shown to specifically modulate the gut microbiome, potentially contributing to health benefits. Noticeably, our findings contradict a recent in vitro study, underscoring the critical role of employing a physiologically relevant digestion/absorption procedure for a more accurate evaluation of the microbiome-modulating potential of complex foods. Full article

Blackcurrant is an anthocyanin-rich berry with proven antioxidant and photoprotective activity and emerging prebiotic potential, widely applied in cosmetic products. Hereby, highly efficient enzyme-assisted extraction of blackcurrant polyphenols was performed, giving extract with very high antioxidant activity. Obtained extract was characterized in terms of anthocyanin composition, incorporated into three different cosmetic formulations and subjected to Franz cell diffusion study. Experimental values obtained using cellulose acetate membrane for all four dominant anthocyanins (delphinidin 3-glucoside, delphinidin 3-rutinoside, cyanidin 3-glucoside and cyanidin 3-rutinoside) were successfully fitted with the Korsmeyer–Peppas diffusion model. Calculated effective diffusion coefficients were higher for hydrogel compared to oil-in-water cream gel and oil-in-water emulsion, whereas the highest value was determined for cyanidin 3-rutinoside. On the other hand, after a 72 h long experiment with transdermal skin diffusion model (Strat-M^® membrane), no anthocyanins were detected in the receptor fluid, and only 0.5% of the initial quantity from the donor compartment was extracted from the membrane itself after experiment with hydrogel. Present study revealed that hydrogel is a suitable carrier system for the topical delivery of blackcurrant anthocyanins, while dermal and transdermal delivery of these molecules is very limited, which implies its applicability for treatments targeting skin surface (i.e., prebiotic, photoprotective). Full article

The goal of the research was to determine the impact of fortification with polyphenolic compounds on (i) sensory attractiveness (global satisfaction, appearance, colour, odour, flavour, sweetness, bitterness), (ii) content of polyphenols and colour (L*, a*, b*) after the baking process and (iii) their bioactive potential (antioxidants activity and inhibiting of α-amylase and α-glucosidase enzyme). Fortification was made with extracts of polyphenolic compounds of selected plant raw materials rich in polyphenols from quince (fruits), tilia (flowers), pomegranate (skin), passion fruit (endocarp), sour cherries (leaves), haskap and chokeberry (berries), silver skin (coffee beans), rosehip (seeds). Depending on the nature of the polyphenol extract, flavan-3-ols (monomeric and polymeric), phenolic acid, flavonols and anthocyanins were identified in the product in amounts ranging from 53.7 to 212.6 mg/100 g DM. Cookies’ colour (L*, a*, b*) depended on the type of polyphenol extract used for fortification. Cookies with haskap, chokeberry and sour cherry presented the highest antioxidant potential. Cookies with chokeberry, haskap and rosehip presented high activity in inhibiting α-amylase (65.5, 60.6 and 62.2% of inhibition, respectively), but cookies with haskap, silver skin and quince in inhibiting α-glucosidase activity (23.0, 20.4 and 21.4% of inhibition, respectively). In the sensory evaluation, the most attractive were cookies with rosehip and pomegranate (6.3 and 5.8 score, respectively), but the lowest ratings were given to cookies with passion fruit and silver skin but especially quince cookies, which obtained the lowest desirability (3.7 score). The acceptability of fortified cookies was determined to the least extent by monomeric flavan-3-ols and phenolic acids (in minus in odour/flavour, bitterness, sweetness and global satisfaction), but anthocyanins, polymeric procyanidins and flavonols had the most significant positive impact on consumer acceptance of the assessed features, i.e., global satisfaction, odour/flavour, sweetness and bitterness (positive consumer drivers). Full article

PIWI, from the German word Pilzwiderstandsfähig, meaning “fungus-resistant”, refers to grapevine cultivars bred for resistance to fungal pathogens such as Erysiphe necator (the causal agent of powdery mildew) and Plasmopara viticola (the causal agent of downy mildew), two major diseases in viticulture. These varieties are typically developed through traditional breeding, often crossbreeding European Vitis vinifera with American or Asian species that carry natural disease resistance. This study investigates the transcriptional profiles of exocarp tissues in mature berries from four PIWI grapevine varieties compared to their elite parental counterparts using RNA-seq analysis. We performed RNA-seq on four PIWI varieties (two red and two white) and their noble parents to identify differential gene expression patterns. Comprehensive analyses, including Differential Gene Expression (DEGs), Gene Set Enrichment Analysis (GSEA), Weighted Gene Co-expression Network Analysis (WGCNA), and tau analysis, revealed distinct gene clusters and individual genes characterizing the transcriptional landscape of PIWI varieties. Differentially expressed genes indicated significant changes in pathways related to organic acid metabolism and membrane transport, potentially contributing to enhanced resilience. WGCNA and k-means clustering highlighted co-expression modules linked to PIWI genotypes and their unique tolerance profiles. Tau analysis identified genes uniquely expressed in specific genotypes, with several already known for their defense roles. These findings offer insights into the molecular mechanisms underlying grapevine resistance and suggest promising avenues for breeding strategies to enhance disease resistance and overall grape quality in viticulture. Full article

Norisoprenoids are important chemical compounds to grape and wine aroma, and their content in the grape berries can be greatly affected by varietal, terroir, and environmental factors. In this study, we investigate how major factors, such as genotype and climate conditions, influence the physicochemical properties of grape juice, volatile C13-norisoprenoid compounds, and gene expression profiles of three Vitis vinifera grape varieties: Muscat blanc à Petit grain, Muscat à petits grains rouges, and Gewürztraminer during the production period in 2010 and 2011. The total soluble solids (TSS) of both Muscat varieties were significantly higher in 2011 compared to 2010, reflecting interannual climatic variations, while Gewürztraminer showed no significant differences. At full maturity, total acid of all three cultivars was consistent between the years, indicating genetic determination. Thirteen norisoprenoids were identified, with Muscat varieties showing consistently higher levels than Gewürztraminer, irrespective of the production year. Varietal differences were significant for 13 out of 14 volatile compounds, and vintage effects were notable for 11 compounds, including key aroma contributors β-damascenone and β-ionone. OPLS-DA analysis highlighted distinct volatile profiles for each variety and vintage, influenced by climatic factors such as precipitation and sunlight hours. Gene expression analysis revealed strong correlations between VvCCD1, VvCCD4a, and VvCCD4b genes and C13-norisoprenoid accumulation, with these genes also implicated in the ABA biosynthesis pathway. Single nucleotide polymorphisms (SNPs) in VvCCD1, VvCCD4a, and VvCCD4b were linked to variations in norisoprenoid content among the cultivars. Altogether, these findings revealed the interaction of genetic and environmental factors in shaping the physicochemical properties for the grape, volatile profiles, and gene expression patterns of grape berries, with significant implications for viticulture and the winemaking process. Full article

The Khanty-Mansi Autonomous Okrug-Yugra, situated within Russia’s Far North, has undergone substantial industrialization and economic development. However, it is confronted with considerable environmental challenges, notably soil contamination. This study examines the presence of heavy metals (lead, cadmium, copper and zinc) in medicinal and berry plants from the forest ecosystem of this region. The following plant species were analyzed: Hypericum perforatum, Rubus arcticus, Origanum vulgare and Thymus vulgaris. The samples were taken from both open ground and hydroponic cultivation under artificial lighting. The findings indicate that the levels of lead present in all samples remain below the permissible limit of 10 mg/kg. Cadmium levels exhibited variability, with hydroponically grown plants containing 0.01 to 0.5 mg/kg and open ground Hypericum and Rubus perforatum containing up to 0.8 mg/kg. The combination of hydroponic cultivation and specific lighting conditions has been demonstrated to reduce lead and cadmium accumulation by a minimum of 1.6 times in comparison to open ground cultivation. The copper content of the samples ranged from 3 to 8 mg/kg, while the zinc content was 1.2–1.5 times higher in the plants grown in the open compared to those grown hydroponically. Notwithstanding these variations, the heavy metal content of all plant samples remains below the threshold values, thus rendering them safe for harvesting and utilization. This research serves to illustrate the environmental impact of industrial activities and to identify hydroponics as a potential strategy for their mitigation. Full article

Emissions of carbon dioxide (CO₂), nitrous oxide (N₂O), and methane (CH₄), particularly those from organic soils, need to be reduced in the context of climate change mitigation (CCM). Here, we estimated the greenhouse gas (GHG) fluxes from nutrient-poor organic soils in cranberry (Vaccinium macrocarpon) and highbush blueberry (Vaccinium corymbosum) plantations established on former peat extraction fields compared to active peat extraction fields and pristine raised bogs in Latvia. A two-year study (2016–2018) was conducted using the manual closed chamber method. In berry plantations and active peat extraction fields, annual net CO₂ fluxes contributed the most to total GHG emissions, accounting for over 67%, and temperature had the most significant impact on CO₂ fluxes. Conversely, annual CH₄ fluxes were the primary contributor to total net GHG emissions in the pristine raised bog, which simultaneously acted as a slight CO₂ sink. N₂O fluxes were relatively low among all studied land use types. This study provided quantitative insights into the variation in GHG fluxes and the environmental variables influencing them, and the obtained data are valuable to estimate the impact of the establishment of berry plantations on former peat extraction fields on CCM in the hemiboreal region of Europe. Full article

The valley degree of freedom, as a new information carrier, is important for basic physical research and the development of advanced devices. Herein, using first-principle calculations, we predict that two-dimensional Hf₂TeIX (X = I, Br) monolayers harbor intriguing valley properties. Without considering spin–orbit coupling (SOC), the Hf₂TeI₂ monolayer has a semi-metallic nature, with Dirac cones located at the high-symmetry point K, and feature, with considerable Fermi velocity. When the SOC is taken into account, a band gap opening of 271 meV can be observed at the Dirac cones. More interestingly, the Hf₂TeIBr monolayer exhibits intrinsic spatial inversion symmetry breaking, which leads to the emergence of valley-contrasting physics under SOC. This is demonstrated by the presence of spin–valley splitting and opposite Berry curvature at adjacent K points. Besides, the spin–valley splitting, the band gap and magnitude of the Berry curvature of the Hf₂TeIBr monolayer can be effectively tuned by strain engineering. These findings contribute significantly to the design of valleytronic devices and extend opportunities for exploring two-dimensional valley materials. Full article

Blueberries offer multiple health benefits, and their cultivation has expanded to warm tropical regions. However, references for foliar nutritional content are lacking in the literature. Proximal sensing may enhance nutritional characterization to optimize blueberry production. We aimed (i) to characterize the nutrient contents of healthy plants of three blueberry varieties adapted to warm climates (Emerald, Jewel, and Biloxi) using a reference method for foliar analysis (inductively coupled plasma (ICP)) and a portable X-ray fluorescence (pXRF) spectrometer on fresh and dry leaves and (ii) to differentiate blueberry varieties based on their nutrient composition. Nutrient content was statistically compared per leaf moisture condition (fresh or dry) with ICP results and used to differentiate the varieties via the random forest algorithm. P and Zn contents (ICP) in leaves were different among varieties. Dry leaf results (pXRF) were strongly correlated with ICP results. Most nutrients determined using ICP presented good correlation with pXRF data (R² from 0.66 to 0.93). The three varieties were accurately differentiated by pXRF results (accuracy: 87%; kappa: 0.80). Predictions of nutrient contents based on dry leaves analyzed by pXRF outperformed those based on fresh leaves. This approach can also be applied to other crops to facilitate nutrient assessment in leaves. Full article

The genus Vaccinium is represented by shrubs growing in a temperate climate that have been used for ages as traditional remedies in the treatment of digestive problems, in diabetes, renal stones or as antiseptics due to the presence of polyphenols (anthocyanins, flavonoids and tannins) in their fruits and leaves. Recent studies confirm their marked potential in the treatment of skin disorders and as skin care cosmetics. The aim of this review is to present the role of Vaccinium spp. as cosmetic products, highlight their potential and prove the biological properties exerted by the extracts from different species that can be useful for the preparation of innovative cosmetics. In the manuscript both skin care and therapeutic applications of the representatives of this gender will be discussed that include the antioxidant, skin lightening, UV-protective, antimicrobial, anti-inflammatory, and chemopreventive properties to shed new light on these underestimated plants. Full article

Delaying an electromagnetic (EM) wave pulse on a thin screen for a significant time before releasing it is highly desired in many applications, such as optical camouflage, information storage, and wave–matter interaction boosting. However, available approaches to achieve this goal either require thick and complex systems or suffer from low efficiencies and a short delay time. This paper proposes an ultra-thin meta-platform that can significantly delay an EM-wave pulse after reflection. Specifically, our meta-platform consists of three meta-surfaces integrated together, of which two are responsible for efficiently coupling incident EM-wave pulse into surface waves (SWs) and vice versa, and the third one supports SWs exhibiting significantly reduced group velocity. We employ theoretical model analyses, full-wave simulations, and microwave experiments to validate the proposed concept. Our experiments demonstrate a 13 ns delay of an EM pulse centered at 12.975 GHz, enabled by a λ/8-thick and 38-λ-long meta-device with an efficiency of 32% (or 70%) with (or without) material loss taken into account. A larger delay time can be enabled by devices with larger sizes considering that the SWs group velocity of our device can be further reduced via dispersion engineering. These findings establish a new road for delaying an EM-wave pulse with ultra-thin screens, which may lead to many promising applications in integration optics. Full article

A wideband polarization-insensitive bistatic radar cross-section (RCS) reduction design under linear and circular polarization incidence is proposed based on spherical-chessboard metasurfaces. A new metasurface element with wideband characteristics was designed, including a double split-ring structure, single-layer media, and metal board. In the proposed RCS-reduction design, the Pancharatnam–Berry (P-B) phase theory is applied with the designed metasurface element to realize phase distribution mimicking the low-scattering sphere, and thus realizing RCS reduction. In addition, the chessboard configuration is combined with spherical phase distribution to further improve the performance of monostatic and bistatic RCS reduction. Finally, the proposed RCS reduction design can not only realize wideband RCS reduction but also exhibit polarization-insensitive characteristics. It realized 10 dB monostatic and bistatic RCS reduction in a frequency band ranging from 8.5 to 21 GHz (84.8% relative bandwidth) under linear polarization (LP) and circular polarization (CP) incidence. The straightforward and efficient design method of the hybrid spherical chessboard can effectively avoid the complex and time-consuming optimization process in RCS-reduction design. Full article

Inadequate nutrient intake, coupled with increased oxidative stress, leads to an imbalance responsible for the current major diseases. Many foods have traditionally been used as traditional medicine, including native berries from southern Chile. Both Maqui and Calafate possess high antioxidant activity, which grants them medicinal power and makes them an excellent alternative for improving health. The aim of this study is to create a functional food with therapeutic properties capable of counteracting oxidative stress and thereby contributing to improving people’s health. To achieve this, a muffin with inulin, Maqui, and Calafate has been developed. The results show that the incorporation of inulin alone increases the fiber content and antioxidant capacity of the muffins; however, Maqui and Calafate contribute significantly more. Furthermore, contents of phenolic compounds are elevated, and an increase in the folic acid content is observed in the samples compared to the control. We can conclude that producing products with inulin, Maqui, and Calafate can be used to enhance the nutritional value and increase the antioxidant activity of bakery products, providing nutrients while also delivering unique characteristics in color, aroma, and flavor, making them appealing to consumers. Full article

Non-alcoholic fatty liver disease (NAFLD) is a common chronic liver disorder marked by the buildup of triacylglycerols (TGs) in the liver. It includes a range of conditions, from simple steatosis to more severe forms like non-alcoholic steatohepatitis (NASH), which can advance to fibrosis, cirrhosis, and hepatocellular carcinoma. NAFLD’s prevalence is rising globally, estimated between 10% and 50%. The disease is linked to comorbidities such as obesity, type 2 diabetes, insulin resistance, and cardiovascular diseases and currently lacks effective treatment options. Therefore, researchers are focusing on evaluating the impact of adjunctive herbal therapies in individuals with NAFLD. One herbal therapy showing positive results in animal models and clinical studies is fruits from the Vaccinium spp. genus. This review presents an overview of the association between consuming fruits, juices, and extracts from Vaccinium spp. and NAFLD. The search used the following keywords: ((Vaccinium OR blueberry OR bilberry OR cranberry) AND (“non-alcoholic fatty liver disease” OR “non-alcoholic steatohepatitis”)). Exclusion criteria included reviews, research notes, book chapters, case studies, and grants. The review included 20 studies: 2 clinical trials and 18 studies on animals and cell lines. The findings indicate that juices and extracts from Vaccinium fruits and leaves have significant potential in addressing NAFLD by improving lipid and glucose metabolism and boosting antioxidant and anti-inflammatory responses. In conclusion, blueberries appear to have the potential to alleviate NAFLD, but more clinical trials are needed to confirm these benefits. Full article

Augmented reality overlays computer-generated virtual information onto real-world scenes, enhancing user interaction and perception. However, traditional augmented reality optical systems are usually large, bulky, and have limited optical performance. In this paper, we propose a novel compact monochrome reflective dielectric metalens visor with see-through properties, engineered using a periodic structure of spin-dependent supercells. The supercell, which is composed of staggered twin nanofins, provides spin-dependent destructive or constructive interference with different circularly polarized incidences. The design combines the principles of interference with the Pancharatnam–Berry phase to enhance reflection at a working wavelength of 650 nm while maintaining good transmission. Right circularly polarized light incident from the substrate side causes destructive interference, enabling the supercell to work in reflection mode, while left circularly polarized light causes constructive interference, enabling the supercell to work in transmission mode. Furthermore, the supercell-constructed metalens can achieve near-diffraction-limited reflective focusing and a broad diagonal field of view of approximately 96°. In addition, compared to transmissive metalens visors, the reflective design eliminates the need for a beam splitter, significantly reducing the size and weight of the system. Our work could facilitate the development of compact and lightweight imaging systems and provide valuable insights for augmented reality near-eye display applications. Full article