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Search Results (4,063)

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Keywords = agri-food

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20 pages, 1240 KiB  
Review
Handling the Imbalanced Problem in Agri-Food Data Analysis
by Adeyemi O. Adegbenjo and Michael O. Ngadi
Foods 2024, 13(20), 3300; https://doi.org/10.3390/foods13203300 - 17 Oct 2024
Abstract
Imbalanced data situations exist in most fields of endeavor. The problem has been identified as a major bottleneck in machine learning/data mining and is becoming a serious issue of concern in food processing applications. Inappropriate analysis of agricultural and food processing data was [...] Read more.
Imbalanced data situations exist in most fields of endeavor. The problem has been identified as a major bottleneck in machine learning/data mining and is becoming a serious issue of concern in food processing applications. Inappropriate analysis of agricultural and food processing data was identified as limiting the robustness of predictive models built from agri-food applications. As a result of rare cases occurring infrequently, classification rules that detect small groups are scarce, so samples belonging to small classes are largely misclassified. Most existing machine learning algorithms including the K-means, decision trees, and support vector machines (SVMs) are not optimal in handling imbalanced data. Consequently, models developed from the analysis of such data are very prone to rejection and non-adoptability in real industrial and commercial settings. This paper showcases the reality of the imbalanced data problem in agri-food applications and therefore proposes some state-of-the-art artificial intelligence algorithm approaches for handling the problem using methods including data resampling, one-class learning, ensemble methods, feature selection, and deep learning techniques. This paper further evaluates existing and newer metrics that are well suited for handling imbalanced data. Rightly analyzing imbalanced data from food processing application research works will improve the accuracy of results and model developments. This will consequently enhance the acceptability and adoptability of innovations/inventions. Full article
(This article belongs to the Special Issue Impacts of Innovative Processing Technologies on Food Quality)
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<p>Receiver operating characteristic (ROC) curves for different classifiers: A—good model, B and C—poor models.</p>
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<p>Typical precision-recall curve for best threshold identification.</p>
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<p>Typical precision-recall curve for optimal model identification (PPV-positive predictive value (precision), SEN- sensitivity (recall), MD1-MD15: Model1-Model15).</p>
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14 pages, 3312 KiB  
Article
Revisiting the Evolution of Multi-Scale Structures of Starches with Different Crystalline Structures During Enzymatic Digestion
by Simin Chen, Zihui Qiu, Ying Yang, Jianfeng Wu, Wenjuan Jiao, Ying Chen and Chengzhi Jin
Foods 2024, 13(20), 3291; https://doi.org/10.3390/foods13203291 - 17 Oct 2024
Abstract
Porous starch has been created through hydrolysis by amyloglucosidase and α-amylase. However, little information is known about the precise evolution of multi-scale structures of starch during digestion. In this study, rice starch and potato starch, containing different crystalline structures, were hydrolyzed by amyloglucosidase [...] Read more.
Porous starch has been created through hydrolysis by amyloglucosidase and α-amylase. However, little information is known about the precise evolution of multi-scale structures of starch during digestion. In this study, rice starch and potato starch, containing different crystalline structures, were hydrolyzed by amyloglucosidase and α-amylase for 20 and 60 min, respectively, and their resulting structural changes were examined. The digestion process caused significant degradation of the molecular structures of rice and potato starches. In addition, the alterations in the ordered structures varied between the two starches. Rice starch exhibited porous structures, thicker crystalline lamellae as determined by small-angle X-ray scattering, and enhanced thermostability after digestion using differential scanning calorimetry. For rice starch, the extent of crystalline structures was analyzed with an X-ray diffractometer; it was found to first increase after 20 min of digestion and then decrease after 60 min of digestion. In contrast, potato starch did not display porous structures but exhibited thicker crystalline lamellae and a reduction in ordered structures after digestion. These findings suggest that it is possible to intentionally modulate the multi-scale structures of starch by controlling the digestion time, thereby providing valuable insights for the manipulation of starch functionalities. Full article
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<p>Morphology of starches. Rice/potato 20/60 refers to the corresponding starch after enzymatic digestion for 20/60 min. The red dotted circles indicate the breakdown of potato starch. The blue arrows indicate the starches that were greatly digested.</p>
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<p>Size distribution of starches.</p>
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<p>Lamellar structures of starches. (<b>A</b>), SAXS curves; (<b>B</b>), Kratky plots; (<b>C</b>) one-dimension correlation profiles.</p>
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<p>X-ray diffraction patterns of starches.</p>
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<p>Changes in the molecular structures of starches. (<b>A</b>), Normalized RI response of the SEC-RI chromatogram of whole starches (normalization was carried out using the individual RI signal of the first eluted peak at around 34–43 min as the normalization factor for each sample); (<b>B</b>), SEC weight chain-length distributions of debranched starches with normalized <span class="html-italic">R<sub>h</sub></span> signals (normalization was performed using the individual signal at Peak I as the normalization factor for each sample); (<b>C</b>), amylose content of starches (columns followed by different letters indicate the data differed significantly in digestion time within the same starch (<span class="html-italic">p &lt;</span> 0.05)).</p>
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<p>Schematic illustration of the changes in multi-scale structures of starches during enzymatic digestion. Rice starch is represented in green, while potato starch is shown in blue.</p>
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10 pages, 7288 KiB  
Communication
Identification and Counting of Pirapitinga Piaractus brachypomus Fingerlings Fish Using Machine Learning
by Alene Santos Souza, Adriano Carvalho Costa, Heyde Francielle do Carmo França, Joel Jorge Nuvunga, Gidélia Araújo Ferreira de Melo, Lessandro do Carmo Lima, Vitória de Vasconcelos Kretschmer, Débora Ázara de Oliveira, Liege Dauny Horn, Isabel Rodrigues de Rezende, Marília Parreira Fernandes, Rafael Vilhena Reis Neto, Rilke Tadeu Fonseca de Freitas, Rodrigo Fortunato de Oliveira, Pedro Henrique Viadanna, Brenno Muller Vitorino and Cibele Silva Minafra
Animals 2024, 14(20), 2999; https://doi.org/10.3390/ani14202999 - 17 Oct 2024
Abstract
Identifying and counting fish are crucial for managing stocking, harvesting, and marketing of farmed fish. Researchers have used convolutional networks for these tasks and explored various approaches to enhance network learning. Batch normalization is one technique that improves network stability and accuracy. This [...] Read more.
Identifying and counting fish are crucial for managing stocking, harvesting, and marketing of farmed fish. Researchers have used convolutional networks for these tasks and explored various approaches to enhance network learning. Batch normalization is one technique that improves network stability and accuracy. This study aimed to evaluate machine learning for identifying and counting pirapitinga Piaractus brachypomus fry with different batch sizes. The researchers used one thousand photographic images of Pirapitinga fingerlings, labeled with bounding boxes. They trained the adapted convolutional network model with batch normalization layers added at the end of each convolution block. They set the training to one hundred and fifty epochs and tested batch sizes of 5, 10, and 20. Furthermore, they measured network performance using precision, recall, and [email protected]. Models with smaller batch sizes performed less effectively. The training with a batch size of 20 achieved the best performance, with a precision of 96.74%, recall of 95.48%, [email protected] of 97.08%, and accuracy of 98%. This indicates that larger batch sizes improve accuracy in detecting and counting pirapitinga fry across different fish densities. Full article
(This article belongs to the Section Aquatic Animals)
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<p>Collection platform scheme. (h) Height in centimeter (cm); (d) Diameter in cm.</p>
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<p>Different densities of pirapitinga fingerlings were used in the study. (<b>A</b>) Tank with 10 fingerlings; (<b>B</b>) Tank with 20 fingerlings; and (<b>C</b>) Tank with 30 fingerlings.</p>
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<p>Bounding boxes around the fish, created using Labelimg.</p>
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<p>Architecture of the convolutional network used in the study. The blocks in blue represent the network’s numerous convolution layers. The grey blocks correspond to the batch normalization (BN) layers that were applied at the end of the convolution blocks. The network starts with the entrance with dimensions 640 × 640 × 3 (height × width × depth). The first part is the backbone and neck, which includes several layers of convolutions and pooling that reduce the spatial dimension of the input image and extract features. The last part is the dense prediction layers which operate in the dimensions 3 × 3 × 1024, allowing for precise and detailed object detection. The blocks representing the convolution layers are connected by arrows, which represent the operations that alter the spatial dimensions, reducing the width and height of the image, maintaining or increasing the number of channels, while the lines indicate the continuous flow of data, preserving the spatial dimensions between successive layers. Adapted from [<a href="#B20-animals-14-02999" class="html-bibr">20</a>].</p>
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<p>Illustrative scheme of the intersection of the manually defined bounding box (light blue) and that predicted by the network (dark blue). When the overlap (IOU) between them is 50%, the convolutional network identifies the fish as correct.</p>
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<p>Confusion matrix. (<b>a</b>) Without normalization; (<b>b</b>) Model with batch size 5; (<b>c</b>) Model with batch size 10; and (<b>d</b>) Model with batch size 20.</p>
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<p>Images with 30 fingerlings detected by the network through mask prediction (light green). Red circles show false positives.</p>
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<p>Fry detected by predicting the delimiting bands using the CNN, in light green. (<b>a</b>) 10 fry detected; (<b>b</b>) 20 fry detected; (<b>c</b>) 30 fry detected; (<b>d</b>) 40 fry detected; (<b>e</b>) 50 fry detected.</p>
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19 pages, 2064 KiB  
Article
Simultaneous and High-Throughput Analytical Strategy of 30 Fluorinated Emerging Pollutants Using UHPLC-MS/MS in the Shrimp Aquaculture System
by Di Huang, Chengbin Liu, Huatian Zhou, Xianli Wang, Qicai Zhang, Xiaoyu Liu, Zhongsheng Deng, Danhe Wang, Yameng Li, Chunxia Yao, Weiguo Song and Qinxiong Rao
Foods 2024, 13(20), 3286; https://doi.org/10.3390/foods13203286 - 16 Oct 2024
Viewed by 226
Abstract
This study established novel and high-throughput strategies for the simultaneous analysis of 30 fluorinated emerging pollutants in different matrices from the shrimp aquaculture system in eastern China using UHPLC-MS/MS. The parameters of SPE for analysis of water samples and of QuEChERS methods for [...] Read more.
This study established novel and high-throughput strategies for the simultaneous analysis of 30 fluorinated emerging pollutants in different matrices from the shrimp aquaculture system in eastern China using UHPLC-MS/MS. The parameters of SPE for analysis of water samples and of QuEChERS methods for sediment and shrimp samples were optimized to allow the simultaneous detection and quantitation of 17 per- and polyfluoroalkyl substances (PFASs) and 13 fluoroquinolones (FQs). Under the optimal conditions, the limits of detection of 30 pollutants for water, sediment, and shrimp samples were 0.01–0.30 ng/L, 0.01–0.22 μg/kg, and 0.01–0.23 μg/kg, respectively, while the limits of quantification were 0.04–1.00 ng/L, 0.03–0.73 μg/kg, and 0.03–0.76 μg/kg, with satisfactory recoveries and intra-day precision. The developed methods were successfully applied to the analysis of multiple samples collected from aquaculture ponds in eastern China. PFASs were detected in all samples with concentration ranges of 0.18–0.77 μg/L in water, 0.13–1.41 μg/kg (dry weight) in sediment, and 0.09–0.96 μg/kg (wet weight) in shrimp, respectively. Only two FQs, ciprofloxacin and enrofloxacin, were found in the sediment and shrimp. In general, this study provides valuable insights into the prevalence of fluorinated emerging contaminants, assisting in the monitoring and control of emerging contaminants in aquatic foods. Full article
(This article belongs to the Section Food Analytical Methods)
22 pages, 1409 KiB  
Review
Studies on Heavy Precipitation in Portugal: A Systematic Review
by José Cruz, Margarida Belo-Pereira, André Fonseca and João A. Santos
Climate 2024, 12(10), 163; https://doi.org/10.3390/cli12100163 (registering DOI) - 15 Oct 2024
Viewed by 355
Abstract
This systematic review, based on an adaptation of the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) statement from 2020, focuses on studies of the atmospheric mechanisms underlying extreme precipitation events in mainland Portugal, as well as observed trends and projections. The [...] Read more.
This systematic review, based on an adaptation of the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) statement from 2020, focuses on studies of the atmospheric mechanisms underlying extreme precipitation events in mainland Portugal, as well as observed trends and projections. The 54 selected articles cover the period from 2000 to 2024, in which the most used keywords are “portugal” and “extreme precipitation”. Of the 54, 23 analyse trends and climate projections of precipitation events, confirming a decrease in total annual precipitation, especially in autumn and spring, accompanied by an increase in the frequency and intensity of extreme precipitation events in autumn, spring and winter. Several articles (twelve) analyse the relationship between synoptic-scale circulation and heavy precipitation, using an atmospheric circulation types approach. Others (two) establish the link with teleconnection patterns, namely the North Atlantic Oscillation (NAO), and still others (three) explore the role of atmospheric rivers. Additionally, five articles focus on evaluating databases and Numerical Weather Prediction (NWP) models, and nine articles focus on precipitation-related extreme weather events, such as tornadoes, hail and lightning activity. Despite significant advances in the study of extreme precipitation events in Portugal, there is still a lack of studies on hourly or sub-hourly scales, which is critical to understanding mesoscale, short-lived events. Several studies show NWP models still have limitations in simulating extreme precipitation events, especially in complex orography areas. Therefore, a better understanding of such events is fundamental to promoting continuous improvements in operational weather forecasting and contributing to more reliable forecasts of such events in the future. Full article
(This article belongs to the Section Weather, Events and Impacts)
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<p>Map of the NUT II administrative divisions of mainland Portugal, and the main rivers.</p>
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<p>Scheme of the methodology applied in the current systematic literature review.</p>
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<p>PRISMA flow diagram of the systematic literature review search adapted from [<a href="#B29-climate-12-00163" class="html-bibr">29</a>].</p>
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<p>Temporal distribution of articles included in the systematic review by publication year.</p>
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<p>Constructed Author’s Keywords (DE) co-occurrence network, used at least twice, using VOSviewer.</p>
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30 pages, 2563 KiB  
Article
Assessing the Opportunities and Risks of DUS and VCU Variety Testing for Sustainable Production through SWOT Analysis Results
by Nóra Mendler-Drienyovszki, Katalin Magyar-Tábori, Roberto Mancinelli, Lisa Black, Hazel Brown, Mohamed Allam, Sripada M. Udupa, Mariam Atait, Elena Novarina, Tommaso Bardelli, Preben Klarskov Hansen, Paul Cottney and Anna Giulini
Agriculture 2024, 14(10), 1817; https://doi.org/10.3390/agriculture14101817 (registering DOI) - 15 Oct 2024
Viewed by 588
Abstract
Within the European Union (EU), new plant varieties to be included in the Common catalog of a member state have to be registered on the national list after plant variety testing processes to establish whether the candidate variety is distinguishable, uniform, and stable [...] Read more.
Within the European Union (EU), new plant varieties to be included in the Common catalog of a member state have to be registered on the national list after plant variety testing processes to establish whether the candidate variety is distinguishable, uniform, and stable (DUS) and meets the cultivation or use value requirement (VCU). Technical development, climate change, and changing consumer needs, including the detection of GMOs, necessitate the innovation of plant variety testing methods. In our study, we assessed new characters, testing methods, and inclusion of additional data for the potential to benefit the DUS and VCU protocols. To achieve our goal, we asked experts to fill in questionnaires for the DUS and VCU methods currently used for a selection of common crops, including potato, maize, lentil, oilseed rape, and perennial grass. Within the EU-funded “InnoVar” project, partners sent out questionnaires to 19 European Countries and to 3 countries outside Europe. Surveys were aimed at analyzing the strengths, weaknesses, opportunities, and threats (SWOT) of the current methods. With their help, it is possible to look for a new direction, opportunity, and strategy to incorporate, together with the innovative new techniques, into the development of the new methods. Our study demonstrated that the SWOT analysis could be used to achieve the set goals. Results obtained after evaluation of surveys confirmed that introduction of new characters such as cold tolerance, nitrogen and water efficiency, etc. has become necessary, as has the inclusion of new test methods (molecular markers, precision techniques, organic farming). The development of high-yielding, disease and/or pest-resistant plant varieties with good adaptability and the accurate evaluation of genotypes play a crucial role in ensuring that farmers can access high-performing plant varieties and contribute to sustainable food production. Full article
(This article belongs to the Section Crop Production)
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<p>Research methodology structure.</p>
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<p>The ear characteristics of durum wheat are important variety traits (Source ‘InnoVar’ experiment, Nyíregyháza, Hungary).</p>
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<p>Uniform and heterogenous seed colors of different lentil breeding lines (Source: photos were taken by Nóra Mendler-Drienyovszki).</p>
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<p>DUS observations of perennial ryegrass (source: photos were taken by Lisa Black).</p>
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<p>Different light-sprouts of potato breeding lines (Source: photos were taken by Katalin Magyar-Tábori).</p>
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<p>Frequency figures of answers to the SWOT questionnaire regarding DUS tests of maize (<b>A</b>), potato (<b>B</b>), perennial ryegrass (<b>C</b>), lentil (<b>D</b>), and oilseed rape (<b>E</b>), where S, W, O, and T mean strengths, weaknesses, opportunities, and threats, respectively. Codes of questions: S1. Do you consider the DUS protocol used conforms to an internationally accepted standard? S2. Scale used for expression level: Do you think it is a Strength? S3. The groups of characteristics: Do you think the characteristic lists complete and so can be considered Strength? S4. The characteristics used for differentiating varieties: Do you think it can be considered Strength? W1. Inaccurate expression categories (e.g., determination of seed color): Do you think it is correct? W2. Lack of total objectivity for not measurable characteristics (visually registered, pseudo-qualitative traits, such as shape, etc.): Do you think it is correct? O1. Use of molecular markers: Does molecular marker testing to be considered an Opportunity? O2. (in the case of maize) Priority of DUS data sharing between European countries. T1. Please suggest here any other aspects or innovations which can be considered! Categories: (1)—Yes; (2)—Yes, conditionally; (3)—Yes, partly; (4)—No; (5)—Not relevant.</p>
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<p>Frequency table of answers to the SWOT questionnaire regarding VCU tests of maize (<b>A</b>), potato (<b>B</b>), perennial ryegrass (<b>C</b>), and oilseed rape (<b>D</b>), where S, W, O, and T mean strengths, weaknesses, opportunities, and threats, respectively. Code of questions: S1: Quality control: Do you consider quality control a Strength in your scientific knowledge? S2: Varieties are tested in organic conditions: Are varieties tested in organic management? S3 (in the case of maize): Do you think adding organic test could be a S? W1: Lack of international standardization on methodologies (e.g., minimum values for content in protein, sugar, fat, etc.): Do you think this is a Weakness? W2: Lack of international standardization in the protocols: Do you think this is a Weakness? W3: Lack of available data. Do you think this is fundamental? W4: Varieties are tested only in conventional management: Do you think this is a Weakness? W5: Number of organic trial locations is low: Is it correct in your Country? W6: Lack of national and international priority of characteristics during the VCU test: Do you think this is a Weakness? O1: Involvement of special traits (e.g., weed competitiveness, nitrogen use efficiency, etc.). Do you think is this an Opportunity? O2: Decrease cost of post-registration tests: Do you think is this an Opportunity if applied? T1: Organic trials are more expensive than the conventional ones: Is it correct in your Country? T2 (in the case of potato and maize): If yes, do you think it should be supported by the government because it could become an Opportunity? T3: Expensive molecular studies: Does molecular testing have a reason to be applied? T4: If yes, do you think it should be supported by the government because it could become an Opportunity? Categories: (1)—Yes; (2)—Yes, conditionally; (3)—Yes, partly; (4)—No; (5)—Not relevant.</p>
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19 pages, 2897 KiB  
Article
Viral Diversity in Mixed Tree Fruit Production Systems Determined through Bee-Mediated Pollen Collection
by Raj Vansia, Malek Smadi, James Phelan, Aiming Wang, Guillaume J. Bilodeau, Stephen F. Pernal, M. Marta Guarna, Michael Rott and Jonathan S. Griffiths
Viruses 2024, 16(10), 1614; https://doi.org/10.3390/v16101614 (registering DOI) - 15 Oct 2024
Viewed by 381
Abstract
Commercially cultivated Prunus species are commonly grown in adjacent or mixed orchards and can be infected with unique or commonly shared viruses. Apple (Malus domestica), another member of the Rosacea and distantly related to Prunus, can share the same growing [...] Read more.
Commercially cultivated Prunus species are commonly grown in adjacent or mixed orchards and can be infected with unique or commonly shared viruses. Apple (Malus domestica), another member of the Rosacea and distantly related to Prunus, can share the same growing regions and common pathogens. Pollen can be a major route for virus transmission, and analysis of the pollen virome in tree fruit orchards can provide insights into these virus pathogen complexes from mixed production sites. Commercial honey bee (Apis mellifera) pollination is essential for improved fruit sets and yields in tree fruit production systems. To better understand the pollen-associated virome in tree fruits, metagenomics-based detection of plant viruses was employed on bee and pollen samples collected at four time points during the peak bloom period of apricot, cherry, peach, and apple trees at one orchard site. Twenty-one unique viruses were detected in samples collected during tree fruit blooms, including prune dwarf virus (PDV) and prunus necrotic ringspot virus (PNRSV) (Genus Ilarvirus, family Bromoviridae), Secoviridae family members tomato necrotic ringspot virus (genus Nepovirus), tobacco necrotic ringspot virus (genus Nepovirus), prunus virus F (genus Fabavirus), and Betaflexiviridae family member cherry virus A (CVA; genus Capillovirus). Viruses were also identified in composite leaf and flower samples to compare the pollen virome with the virome associated with vegetative tissues. At all four time points, a greater diversity of viruses was detected in the bee and pollen samples. Finally, the nucleotide sequence diversity of the coat protein regions of CVA, PDV, and PNRSV was profiled from this site, demonstrating a wide range of sequence diversity in pollen samples from this site. These results demonstrate the benefits of area-wide monitoring through bee pollination activities and provide new insights into the diversity of viruses in tree fruit pollination ecosystems. Full article
(This article belongs to the Special Issue Plant Virus Spillovers)
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<p>Map diagram of tree fruits grown on AAFC Jordan Farm and sampling details. (<b>A</b>) Map diagram of AAFC Jordan Farm. Honey bee icons indicate the location of colonies used for sampling. Fruit diagrams indicate the location of plots used to grow apricots (yellow), cherries (red), peaches (orange), and apples (green). Blue stars indicate which plots were sampled for leaf and flower tissue. (<b>B</b>) Collected pollen. (<b>C</b>) Forager bee on an apple flower. (<b>D</b>) Hive bees collected from inside the colony. (<b>E</b>) Approximate bloom levels of apricot, cherry, peach, and apple trees over time. Bee icons indicate approximate sampling times.</p>
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<p>Virus species detection frequency and diversity. (<b>A</b>) Average number of virus species detected in forager, hive bees, or pollen samples collected during apricot, cherry, peach, and apple blooms. Error bars indicate standard error. The asterisk denotes a significant difference (one-way ANOVA, Tukey-HSD, <span class="html-italic">p</span> &lt; 0.05). (<b>B</b>) Venn diagram of virus species detected during apricot, cherry, peach, and apple blooms. (<b>C</b>) Frequency of detection of CVA, PDV, and PNRSV during apricot, cherry, peach, and apple blooms.</p>
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<p>Venn diagrams of crop and sample type viral diversity. (<b>A</b>) Viral diversity in apricot, cherry, peach, and apple samples. (<b>B</b>) Viral species diversity in different sample types. (<b>B</b>–<b>E</b>) Viruses detected in bee-collected samples and plant samples for (<b>B</b>) apricots, (<b>C</b>) cherries, (<b>D</b>) peaches, and (<b>E</b>) apples.</p>
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<p>CVA CP nucleotide sequence diversity. (<b>A</b>) Pairwise identity of nucleotide CP sequences, with NC_003689 as a reference sequence. (<b>B</b>) Maximum likelihood phylogenetic tree of CVA CP sequences. Scale bars and corresponding numbers indicate the average number of mutations per base. The asterisk indicates these branches are not to scale. Numbers indicate support for branches; only branch points with over 70% support are shown. Orange indicates apricot samples, red for cherry, yellow for peach, and green for apple.</p>
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<p>PDV CP nucleotide sequence diversity. (<b>A</b>) Pairwise identity of nucleotide CP sequences, with NC_008038 as a reference sequence. (<b>B</b>) Maximum likelihood phylogenetic tree of PDV CP sequences. Scale bars and corresponding numbers indicate the average number of mutations per base. The asterisk indicates these branches are not to scale. Numbers indicate support for branches; only branch points with over 70% support are shown. Orange indicates apricot samples, red for cherry, yellow for peach, and green for apple.</p>
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<p>PNRSV CP nucleotide sequence diversity. (<b>A</b>) Pairwise identity of CP sequences, with NC_004364 as a reference sequence. (<b>B</b>) Maximum likelihood phylogenetic tree of PNRSV CP sequences. Scale bars and corresponding numbers indicate the average number of mutations per base. The asterisk indicates these branches are not to scale. Numbers indicate support for branches; only branch points with over 70% support are shown. Orange indicates apricot samples, red for cherry, yellow for peach, and green for apple. Complete PDV CP sequences were obtained from 26 sample datasets, including plant tissue samples from cherry (n = 2) (<a href="#viruses-16-01614-f005" class="html-fig">Figure 5</a>A; <a href="#app1-viruses-16-01614" class="html-app">Supplemental Table S1</a>). Two branches of sequences derived from these samples were identified, all within PDV phylogroup II, as defined in Kinoti et al., 2018 (<a href="#viruses-16-01614-f005" class="html-fig">Figure 5</a>B) [<a href="#B44-viruses-16-01614" class="html-bibr">44</a>]. The first branch contained nine sequences derived from peach (n = 4), cherry (n = 3, two from leaf/flower samples), and apricot (n = 2) and clustered closely with the reference sequence (NC_008038). Identities within this branch ranged from 97.9–100% (<a href="#viruses-16-01614-f005" class="html-fig">Figure 5</a>B). The second branch contained eight sequences with identities ranging from 99.2–100% and clustered closely with an isolate from a Bulgarian sweet cherry sample (MK139682; <a href="#viruses-16-01614-f005" class="html-fig">Figure 5</a>) [<a href="#B45-viruses-16-01614" class="html-bibr">45</a>]. Sequence identity was typically over 98% within each branch and less than 97.6% identical between branches (<a href="#viruses-16-01614-f005" class="html-fig">Figure 5</a>A). Five other sequences derived from apricot and cherry samples branched more independently (<a href="#viruses-16-01614-f005" class="html-fig">Figure 5</a>B).</p>
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25 pages, 26385 KiB  
Article
An Innovative Tool for Monitoring Mangrove Forest Dynamics in Cuba Using Remote Sensing and WebGIS Technologies: SIGMEM
by Alexey Valero-Jorge, Raúl González-Lozano, Roberto González-De Zayas, Felipe Matos-Pupo, Rogert Sorí and Milica Stojanovic
Remote Sens. 2024, 16(20), 3802; https://doi.org/10.3390/rs16203802 - 12 Oct 2024
Viewed by 439
Abstract
The main objective of this work was to develop a viewer with web output, through which the changes experienced by the mangroves of the Gran Humedal del Norte de Ciego de Avila (GHNCA) can be evaluated from remote sensors, contributing to the understanding [...] Read more.
The main objective of this work was to develop a viewer with web output, through which the changes experienced by the mangroves of the Gran Humedal del Norte de Ciego de Avila (GHNCA) can be evaluated from remote sensors, contributing to the understanding of the spatiotemporal variability of their vegetative dynamics. The achievement of this objective is supported by the use of open-source technologies such as MapStore, GeoServer and Django, as well as Google Earth Engine, which combine to offer a robust and technologically independent solution to the problem. In this context, it was decided to adopt an action model aimed at automating the workflow steps related to data preprocessing, downloading, and publishing. A visualizer with web output (Geospatial System for Monitoring Mangrove Ecosystems or SIGMEM) is developed for the first time, evaluating changes in an area of central Cuba from different vegetation indices. The evaluation of the machine learning classifiers Random Forest and Naive Bayes for the automated mapping of mangroves highlighted the ability of Random Forest to discriminate between areas occupied by mangroves and other coverages with an Overall Accuracy (OA) of 94.11%, surpassing the 89.85% of Naive Bayes. The estimated net change based on the year 2020 of the areas determined during the classification process showed a decrease of 5138.17 ha in the year 2023 and 2831.76 ha in the year 2022. This tool will be fundamental for researchers, decision makers, and students, contributing to new research proposals and sustainable management of mangroves in Cuba and the Caribbean. Full article
(This article belongs to the Special Issue Remote Sensing: 15th Anniversary)
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<p>Location of the Gran Humedal del Norte de Ciego de Ávila (GHNCA), Cuba.</p>
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<p>General workflow for the development of the WebGis platform: SIGMEM.</p>
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<p>Spatial distribution of the reference points taken in the GHNCA. Green dots indicate mangrove class and red dots non-mangrove.</p>
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<p>Distribution of predictor variables used in the classification model grouped by classes (mangrove/non-mangrove). Selected Sentinel-2 spectral bands and spectral indices selected by the recursive variable elimination method.</p>
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<p>Diagram of the web architecture used for the development of the GeoServer.</p>
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<p>Estimated mangrove areas in the GHN in Ciego de Avila, Cuba emulating Sentinel-2 images. (<b>A</b>) 2020, (<b>B</b>) 2021, (<b>C</b>) 2022, and (<b>D</b>) 2023. Legend: the areas occupied by mangrove ecosystems in each of the years are shown in red; the limits of the GHN of Ciego de Avila are shown in blue dashed lines.</p>
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<p>Two-dimensional view of the workspace within the MapStore application. The red line represents the limit of the GHNCA.</p>
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<p>Three-dimensional view of the workspace within the MapStore application. The red line represents the limit of the GHNCA.</p>
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<p>Access to the metadata catalog of geospatial resources. The red line represents the limit of the GHNCA.</p>
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<p>Functionality for visual intercomparison of layers. The red line represents the limit of the GHNCA.</p>
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<p>Features for viewing and manipulating layer attributes. The red line represents the limit of the GHNCA.</p>
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<p>Vegetation Indices calculated in the GHN of Ciego de Avila, Cuba during the third quarter of the year 2023 emulating Sentinel-2 images. (<b>A</b>) NDVI, (<b>B</b>) EVI, (<b>C</b>) NDMI, and (<b>D</b>) CCCI.</p>
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21 pages, 725 KiB  
Review
The Application of Fungi and Their Secondary Metabolites in Aquaculture
by Abigail John Onomu and Grace Emily Okuthe
J. Fungi 2024, 10(10), 711; https://doi.org/10.3390/jof10100711 - 11 Oct 2024
Viewed by 492
Abstract
Ensuring sustainability has increasingly become a significant concern not only in aquaculture but in the general agrifood sector. Therefore, it is imperative to investigate pathways to feed substitutes/best practices to enhance aquaculture sustainability. The application of fungi in aquaculture provides innovative methods to [...] Read more.
Ensuring sustainability has increasingly become a significant concern not only in aquaculture but in the general agrifood sector. Therefore, it is imperative to investigate pathways to feed substitutes/best practices to enhance aquaculture sustainability. The application of fungi in aquaculture provides innovative methods to enhance the sustainability and productivity of aquaculture. Fungi play numerous roles in aquaculture, including growth, immunity enhancement and disease resistance. They also play a role in bioremediation of waste and bioflocculation. The application of fungi improves the suitability and utilization of terrestrial plant ingredients in aquaculture by reducing the fibre fractions and anti-nutritional factors and increasing the nutrients and mineral contents of plant ingredients. Fungi are good flotation agents and can enhance the buoyancy of aquafeed. Pigments from fungi enhance the colouration of fish fillets, making them more attractive to consumers. This paper, via the relevant literature, explores the multifaceted roles of fungi in aquaculture, emphasizing their potential to transform aquaculture through environmentally friendly and sustainable techniques. The effectiveness of fungi in reducing fibre fractions and enhancing nutrient availability is influenced by the duration of fermentation and the dosage administered, which may differ for various feed ingredients, making it difficult for most aquaculture farmers to apply fungi approximately. Therefore, the most effective dosage and fermentation duration for each feed ingredient should be investigated. Full article
(This article belongs to the Special Issue Fungal Biotechnology and Application 3.0)
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<p>The potential application of fungi in aquaculture.</p>
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20 pages, 4516 KiB  
Article
By-Products Valorization: Peptide Fractions from Milk Permeate Exert Antioxidant Activity in Cellular and In Vivo Models
by Valeria Scalcon, Federico Fiorese, Marica Albanesi, Alessandra Folda, Gianfranco Betti, Marco Bellamio, Emiliano Feller, Claudia Lodovichi, Giorgio Arrigoni, Oriano Marin and Maria Pia Rigobello
Antioxidants 2024, 13(10), 1221; https://doi.org/10.3390/antiox13101221 - 10 Oct 2024
Viewed by 357
Abstract
The discarding of agri-food by-products is a stringent problem due to their high environmental impact. Recovery strategies can lead to a reduction of waste and result in new applications. Agri-food waste represents a source of bioactive molecules, which could promote health benefits. The [...] Read more.
The discarding of agri-food by-products is a stringent problem due to their high environmental impact. Recovery strategies can lead to a reduction of waste and result in new applications. Agri-food waste represents a source of bioactive molecules, which could promote health benefits. The primary goal of this research has been the assessment of the antioxidant activity of milk permeate, a dairy farm by-product, and the isolation and identification of peptide fractions endowed with antioxidant activity. The chromatographic extraction of the peptide fractions was carried out, and the peptides were identified by mass spectrometry. The fractions showed radical scavenging activity in vitro. Moreover, the results in the Caco-2 cell model demonstrated that the peptide fractions were able to protect from oxidative stress by stimulating the Keap1/Nrf2 antioxidant signaling pathway, increasing the transcription of antioxidant enzymes. In addition, the bioactive peptides can affect cellular metabolism, increasing mitochondrial respiration. The action of the peptide fractions was also assessed in vivo on a zebrafish model and resulted in the protection of the whole organism from the adverse effects of acute cold stress, highlighting their strong capability to protect from an oxidative insult. Altogether, the results unveil novel recovery strategies for food by-products as sources of antioxidant bioactive peptides that might be utilized for the development of functional foods. Full article
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<p>Antioxidant activity of PFs obtained from MP. (<b>A</b>) ABTS scavenging activity is reported as Trolox C equivalent antioxidant capacity (TEAC); (<b>B</b>) DPPH scavenging assay; results are reported as percentage with respect to the control (Cnt); (<b>C</b>) estimation of total phenolic content expressed as gallic acid equivalent (GAE). Results are the mean ± SD of three replicates.</p>
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<p>Protective effect of PFs obtained from milk permeate on oxidative stress induction in Caco-2 cells. (<b>A</b>) Effects of the PFs on cell viability. Caco-2 cells were treated with the indicated fractions for 24 h and oxidative stress was induced by 180 µM TbOOH (for 18 h). Results are shown as percentage of cell viability with respect to the Cnt; (<b>B</b>) Estimation of ROS production in Caco-2 cells treated with the indicated PFs for 24 h in the absence (grey) or presence (red) of 300 µM TbOOH and expressed as percentage with respect to the Cnt. Results are the mean ± SD of three replicates ** <span class="html-italic">p</span> &lt; 0.01; *** <span class="html-italic">p</span> &lt; 0.001.</p>
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<p>Nrf2 and NF-κB levels in the nuclear fraction of Caco-2 cells treated with 5–30% ACN PF from MP. Nuclear fractions of cells treated with 0.05 mg/mL of 5–30% ACN PF and/or with 2 mM NAC for 24 h were extracted, and Western blot analysis was carried out to estimate Nrf2, NF-κB, and PCNA levels. (<b>A</b>) Representative WB of protein expression in Caco-2 cells in the different conditions. (<b>Aˈ</b>) Quantitative analysis of the WB after normalization using PCNA as a nuclear loading control. Results are the mean ± SD of three replicates * <span class="html-italic">p</span> &lt; 0.05; *** <span class="html-italic">p</span> &lt; 0.001.</p>
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<p>Expression of antioxidant enzymes in Caco-2 cells treated with 5–30% ACN PF from MP. Cells were treated with 0.05 mg/mL of 5–30% ACN PF for 24 h. Afterwards, cells were lysed, and WB analysis was carried out. (<b>A</b>) Representative images of protein expression of the various enzymes in Caco-2 cells via WB; (<b>B</b>) Ponceau S staining reporting the protein loading; (<b>C</b>) Quantitative analysis of the Western blot after normalization using GAPDH and β-actin as loading controls. Results are the mean ± SD of three replicates * <span class="html-italic">p</span> &lt; 0.05; ** <span class="html-italic">p</span> &lt; 0.01.</p>
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<p>Oxygen consumption rates and glycolytic activity of Caco-2 cells treated with the PF. Caco-2 cells were treated with 0.05 mg/mL of the 5–30% ACN PF for 24 h. (<b>A</b>) The oxygen consumption rates (OCRs) were assessed using the Seahorse Xfe24 analyzer as described in the <a href="#sec2-antioxidants-13-01221" class="html-sec">Section 2</a>. Basal respiration and respiratory capacity in the presence of sequential addition of 1 µM oligomycin, 0.5 µM FCCP and the combination of 1 μM antimycin A + 1 µM rotenone, was measured. (<b>Aˈ</b>) Basal, ATP-linked and maximal respirations are shown as the mean ± SD of 5 experiments, ** <span class="html-italic">p</span> &lt; 0.01, *** <span class="html-italic">p</span> &lt; 0.001. (<b>B</b>) Cellular glycolytic activity was determined in the presence of sequential addition of 10 mM glucose, 1 μM antimycin A + 1 µM rotenone, and 2-deoxy-D-glucose. (<b>Bˈ</b>) Glycolysis, maximal glycolytic capacity, and glycolytic reserve are reported as the mean ± SD of 3 experiments.</p>
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<p>Effects of the 5–30% ACN PF in vivo on zebrafish larvae under cold stress conditions. Zebrafish larvae were divided into four groups: control (placed in 28 °C water) (blue box), acute cold stressed (placed in 10 °C water for 5 min) (pink box), acute cold stressed + 30 min recovery (green box), and acute cold stressed + 30 min recovery in the presence of the PF (yellow box). The motility of the four groups of larvae was assessed using the DanioVision system. (<b>A</b>) Total swimming distance; (<b>B</b>) Average swimming speed. (<span class="html-italic">n</span> = 75, distributed as 25 controls, 13 cold stress, 19 cold stress + recovery, and 18 cold stress + recovery + 5–30% ACN PF), * <span class="html-italic">p</span> &lt; 0.05, ** <span class="html-italic">p</span> &lt; 0.01, *** <span class="html-italic">p</span> &lt; 0.001.</p>
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23 pages, 4465 KiB  
Article
How Climate Change Will Shape Pesticide Application in Quebec’s Golf Courses: Insights with Deep Learning Based on Assessing CMIP5 and CMIP6
by Isa Ebtehaj, Josée Fortin, Hossein Bonakdari and Guillaume Grégoire
Appl. Sci. 2024, 14(20), 9209; https://doi.org/10.3390/app14209209 - 10 Oct 2024
Viewed by 423
Abstract
The accelerating impact of climate change on golf course conditions has led to a significant increase in pesticide dependency, underscoring the importance of innovative management strategies. The shift from Coupled Model Intercomparison Project Phase 5 (CMIP5) to the latest CMIP6 phase has drawn [...] Read more.
The accelerating impact of climate change on golf course conditions has led to a significant increase in pesticide dependency, underscoring the importance of innovative management strategies. The shift from Coupled Model Intercomparison Project Phase 5 (CMIP5) to the latest CMIP6 phase has drawn the attention of professionals, including engineers, decision makers, and golf course managers. This study evaluates how climate projections from CMIP6, using Canadian Earth System Models (CanESM2 and CanESM5), impact pesticide application trends on Quebec’s golf courses. Through the comparison of temperature and precipitation projections, it was found that a more substantial decline in precipitation is exhibited by CanESM2 compared to CanESM5, while the latter projects higher temperature increases. A comparison between historical and projected pesticide use revealed that, in most scenarios and projected periods, the projected pesticide use was substantially higher, surpassing past usage levels. Additionally, in comparing the two climate change models, CanESM2 consistently projected higher pesticide use across various scenarios and projected periods, except for RCP2.6, which was 27% lower than SSP1-2.6 in the second projected period (PP2). For all commonly used pesticides, the projected usage levels in every projected period, according to climate change models, surpass historical levels. When comparing the two climate models, CanESM5 consistently forecasted greater pesticide use for fungicides, with a difference ranging from 65% to 222%, and for herbicides, with a difference ranging from 114% to 247%, across all projected periods. In contrast, insecticides, growth regulators, and rodenticides displayed higher AAIR values in CanESM2 during PP1 and PP3, showing a difference of 28% to 35.6%. However, CanESM5 again projected higher values in PP2, with a difference of 1.5% to 14%. Full article
(This article belongs to the Section Computing and Artificial Intelligence)
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<p>Spatial comparison of historical and climate change modeling results for total precipitation (mm) and average temperature (°C) during the golf season in the province of Quebec (from May to November). In all maps, the star indicates the location of Quebec City. The color gradients represent the range of values, with darker shades of green and yellow representing lower values and orange to red indicating higher values. The scale bars represent the distance in kilometers.</p>
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<p>Distribution of the total precipitation (<b>A</b>) and average temperature (<b>B</b>) during the golf season in the province of Quebec (May to November) across historical data (yellow) and projected climate change models (CanESM2 (light orange) and CanESM5 (cyan)).</p>
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<p>Comparison of pesticide use (in kilograms) calculated based on CanESM2 and CanESM5 climate models across three projected periods (PP1, PP2, and PP3, represented by blue bars) and total pesticide use (represented by red bars). The scenarios compared include RCP2.6, RCP4.5, and RCP8.5 for CanESM2 and SSP1-2.6, SSP2-4.5, and SSP5-8.5 for CanESM5. Historical pesticide use (green bar) is included as a reference.</p>
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<p>The relative difference in pesticide use between historical data and projected use for 2023–2048 is based on the CanESM2 and CanESM5 across three scenarios. The figures show the spatial distribution of relative difference (RD, in %) across golf courses in the province of Quebec. The color gradient indicates the relative difference in pesticide use: green (0–50%), yellow (200–500%), and red (2000–5000%). The star denotes Quebec City, and the black dots indicate the locations of golf courses across the province.</p>
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<p>The relative difference in pesticide use between historical data and projected use for 2049–2074 is based on the CanESM2 and CanESM5 across three scenarios. The figures show the spatial distribution of relative difference (RD, in %) across golf courses in the province of Quebec. The color gradient indicates the relative difference in pesticide use: green (0–50%), yellow (200–500%), and red (2000–5000%). The star denotes Quebec City, and the black dots indicate the locations of golf courses across the province.</p>
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<p>The relative difference in pesticide use between historical data and projected use for 2075–2100 is based on the CanESM2 and CanESM5 across three scenarios. The figures show the spatial distribution of relative difference (RD, in %) across golf courses in the province of Quebec. The color gradient indicates the relative difference in pesticide use: green (0–50%), yellow (200–500%), and red (2000–5000%). The star denotes Quebec City, and the black dots indicate the locations of golf courses across the province.</p>
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<p>Comparison of the observed (historical) and projected average pesticide use (in kilograms) for different pesticide types across various projected periods (PP1, PP2, and PP3) based on CanESM2 and CanESM5 climate models (F: fungicides, H: herbicides, I: insecticides, RC: growth regulators, Ro: rodenticides, and A: others).</p>
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25 pages, 3187 KiB  
Article
Characterization of Unfractionated Polysaccharides in Brown Seaweed by Methylation-GC-MS-Based Linkage Analysis
by Barinder Bajwa, Xiaohui Xing, Spencer C. Serin, Maria Hayes, Stephanie A. Terry, Robert J. Gruninger and D. Wade Abbott
Mar. Drugs 2024, 22(10), 464; https://doi.org/10.3390/md22100464 - 9 Oct 2024
Viewed by 940
Abstract
This study introduces a novel approach to analyze glycosidic linkages in unfractionated polysaccharides from alcohol-insoluble residues (AIRs) of five brown seaweed species. GC-MS analysis of partially methylated alditol acetates (PMAAs) enables monitoring and comparison of structural variations across different species, harvest years, and [...] Read more.
This study introduces a novel approach to analyze glycosidic linkages in unfractionated polysaccharides from alcohol-insoluble residues (AIRs) of five brown seaweed species. GC-MS analysis of partially methylated alditol acetates (PMAAs) enables monitoring and comparison of structural variations across different species, harvest years, and tissues with and without blanching treatments. The method detects a wide array of fucose linkages, highlighting the structural diversity in glycosidic linkages and sulfation position in fucose-containing sulfated polysaccharides. Additionally, this technique enhances cellulose quantitation, overcoming the limitations of traditional monosaccharide composition analysis that typically underestimates cellulose abundance due to incomplete hydrolysis of crystalline cellulose. The introduction of a weak methanolysis-sodium borodeuteride reduction pretreatment allows for the detection and quantitation of uronic acid linkages in alginates. Full article
(This article belongs to the Special Issue High-Value Algae Products)
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<p>GC-TIC chromatograms of PMAAs from the AIRs of HE: (<b>A</b>) without the pretreatment of weak methanolysis-sodium borodeuteride reduction before methylation and (<b>B</b>) pretreated with weak methanolysis-sodium borodeuteride reduction before methylation.</p>
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<p>EI-MS spectra and ion fragmentation patterns of PMAAs from (<b>A</b>) 4-Gul<span class="html-italic">p</span>A, (<b>B</b>) 4-Man<span class="html-italic">p</span>A, (<b>C</b>) 3-Fuc<span class="html-italic">p</span>, (<b>D</b>) 4-Fuc<span class="html-italic">p</span>, (<b>E</b>) 2,3-Fuc<span class="html-italic">p</span>, (<b>F</b>) 3,4-Fuc<span class="html-italic">p</span>, (<b>G</b>) 2,4-Fuc<span class="html-italic">p</span>, and (<b>H</b>) 2,3,4-Fuc<span class="html-italic">p</span> in HE.</p>
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<p>Relative compositions of (<b>A</b>) monosaccharides and (<b>B</b>) polysaccharides calculated from linkage compositions of AIRs of five brown seaweed species. UA: uronic acids; Man: mannose; Xyl: xylose; Gal: galactose; Glc: glucose; Fuc: fucose; Rha: rhamnose; Ara: arabinose; NA: unassigned linkages; CE: cellulose; LM: laminarin; SF: sulfated fucan. HE was harvested in Q4 of 2020, and FV was harvested in 2020. AM, MT, and SL were harvested in Q2 of 2021. All samples were unblanched.</p>
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<p>Relative compositions of (<b>A</b>) monosaccharides and (<b>B</b>) polysaccharides calculated from linkage compositions of AIRs of the receptacle, blade, and stipe of MT harvested in 2021 without blanching. UA: uronic acids; Man: mannose; Xyl: xylose; Gal: galactose; Glc: glucose; Fuc: fucose; Rha: rhamnose; Ara: arabinose; NA: unassigned linkages; CE: cellulose; LM: laminarin; SF: sulfated fucan; AL: alginate.</p>
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<p>Relative compositions of (<b>A</b>) monosaccharides and (<b>B</b>) polysaccharides calculated from linkage compositions of AIRs of AM and SL in 2021 and 2022. UA: uronic acids; Man: mannose; Xyl: xylose; Gal: galactose; Glc: glucose; Fuc: fucose; Rha: rhamnose; Ara: arabinose; NA: unassigned linkages; CE: cellulose; LM: laminarin; SF: sulfated fucan; AL: alginate.</p>
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<p>Bubble plots showing fold changes in glycosidic linkages: (<b>A</b>) between 2021 and 2022 harvests of AM and SL and (<b>B</b>) between blanched and unblanched samples of AM, SL, and the receptacle (R), blade (B), and stipe (S) of MT harvested in 2021. Fold values were calculated as the ratio of the maximum to minimum of each pair of compositions for each linkage, excluding trace-level linkages. Bubble size represents fold value, while bubble color indicates the difference in the pair: coral signifies higher linkage compositions in 2021 compared to 2022 in panel <b>A</b> and in unblanched compared to blanched samples in panel <b>B</b>, while turquoise indicates the opposite.</p>
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<p>Relative compositions of (<b>A</b>) monosaccharides and (<b>B</b>) polysaccharides calculated from linkage compositions of AIRs of blanched and unblanched samples of AM and SL harvested in 2021. UA: uronic acids; Man: mannose; Xyl: xylose; Gal: galactose; Glc: glucose; Fuc: fucose; Rha: rhamnose; Ara: arabinose; NA: unassigned linkages; CE: cellulose; LM: laminarin; SF: sulfated fucan; AL: alginate. B and U represent blanched and unblanched samples, respectively.</p>
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23 pages, 1279 KiB  
Review
Legal Barriers in Sustainable Agriculture: Valorization of Agri-Food Waste and Pesticide Use Reduction
by Rosalinda Nicastro, Mattia Papale, Giovanna Marta Fusco, Annalinda Capone, Biagio Morrone and Petronia Carillo
Sustainability 2024, 16(19), 8677; https://doi.org/10.3390/su16198677 - 8 Oct 2024
Viewed by 835
Abstract
The transition to sustainability in agriculture faces significant challenges, especially to balance environmental goals with the practical demands of food production. This paper examines two different case studies that reveal the complexities of agricultural regulation. The first case focuses on the valorization of [...] Read more.
The transition to sustainability in agriculture faces significant challenges, especially to balance environmental goals with the practical demands of food production. This paper examines two different case studies that reveal the complexities of agricultural regulation. The first case focuses on the valorization of agri-food residual biomasses, highlighting the potential to transform food waste into valuable bioproducts such as bioenergy and biofertilizers. Despite the clear environmental and economic benefits, the absence of specific European regulations hinders the widespread adoption of these practices. Without clear rules for achieving “end-of-waste” status, the development and marketing of bio-based products remain restricted. The second case study examines the European Union’s unsuccessful effort to implement the Sustainable Use of Pesticides Regulation (SUR), which aimed to reduce pesticide use by 50% by 2030. Although the regulation sought to align agricultural practices with the EU’s Green Deal, it triggered widespread protests from farmers concerned about the potential economic losses and decreased productivity. These two cases, one showing under-regulation and the other over-regulation, highlight the need for balanced and practical regulatory frameworks that promote sustainability without imposing unrealistic demands on stakeholders. This paper ends with recommendations to harmonize regulations across Europe, ensuring that both innovation in agricultural waste management and practical pesticide reduction strategies are implemented in a way that supports farmers and producers, minimizing economic disruptions and encouraging sustainable agricultural practices. Full article
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<p>Waste hierarchy inverted pyramid.</p>
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<p>The circular economy’s strategy is to produce no waste because today’s residual products are tomorrow’s raw materials.</p>
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<p>The variation in the price of fertilizers aggregated by nutrient in Europe from January 2021 to July 2024. Image created using data from Agri-food market data [<a href="#B77-sustainability-16-08677" class="html-bibr">77</a>].</p>
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29 pages, 9703 KiB  
Article
Bibliometric Trends and Insights into the Potential of Maize (Zea mays) under the Framework of Conservation Agriculture
by Smruti Ranjan Padhan, Sushmita Saini, Shankar Lal Jat, Sanjay Singh Rathore, Mahesh Kumar Gathala, Radheshyam, Soumya Ranjan Padhan, Salah El-Hendawy and Mohamed A. Mattar
Sustainability 2024, 16(19), 8670; https://doi.org/10.3390/su16198670 - 8 Oct 2024
Viewed by 759
Abstract
In spite of the detrimental effects of climate change and decreasing resource efficiency, maize farming is essential to the world’s food and nutritional security. With regard to sustainable maize farming in this environment, conservation agriculture (CA) offers a framework that holds promise in [...] Read more.
In spite of the detrimental effects of climate change and decreasing resource efficiency, maize farming is essential to the world’s food and nutritional security. With regard to sustainable maize farming in this environment, conservation agriculture (CA) offers a framework that holds promise in terms of low soil disturbance, perennial soil cover, and sustainable crop rotation. In order to acquire more profound information on the research advancements and publication patterns related to maize under CA scenarios, a bibliometric analysis was conducted. This involved utilizing René Descartes’s Discourse Framework to extract and screen 2587 documents spanning the years 2001 to 2023 from the Dimensions.ai database. The mapping showed that different stakeholders were becoming more interested in maize research under various CA pathways, with a greater emphasis on reaching the second sustainable development target, or “zero hunger”. The most influential journals were “Soil and Tillage Research” and “Field Crops Research”, with 131 and 85 papers with 6861 and 6186 citations, respectively. The performance analysis found “Christian L. Thierfelder” and “Mangi Lal Jat” as the eminent researchers in the areas of maize research under CA. Thus, the International Maize and Wheat Improvement Center (CIMMYT) and the Indian Agricultural Research Institute (IARI) were identified as the important institutions in conducting research pertaining to maize under CA systems, while the United States, India, and Mexico emerged as prominent countries with notable collaboration efforts for imparting research under the given scenarios. Three thematic clusters delineating keywords from three distinct sections—key drivers, objectives, and methodology—were identified through co-word analysis using word clouds, tree maps, and thematic networking of the keywords from the abstract and titles of screened publications. These thematic clusters highlighted the growing emphasis on region-specific studies under CA, particularly in sub-Saharan Africa and the Indo-Gangetic plain, to enhance the resilience of the agri-food system. Therefore, mapping maize’s potentialities within the CA framework has revealed the field’s dynamic nature and offers insightful information to researchers and policymakers that could help them plan future studies and cooperative initiatives aimed at boosting the productivity and sustainability of maize-based systems under the CA framework. Full article
(This article belongs to the Special Issue Land Management and Sustainable Agricultural Production: 2nd Edition)
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<p>Detailed steps of René Descartes’s Discourse Framework.</p>
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<p>Growth of research publications on maize under conservation agriculture from the year 2001 to 2023.</p>
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<p>Scientific production of countries involved in maize research publications under conservation agriculture between 2001 and 2023 (light to deep blue shades signify lower to higher numbers of publications).</p>
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<p>The number of publications related to maize under conservation agriculture systems highlighting various domains from 2001 to 2023.</p>
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<p>The number of publications related to maize under conservation agriculture systems signifying each SDG theme from 2001 to 2023.</p>
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<p>Citation analysis of authors of maize research publications under conservation agriculture between 2001 and 2023.</p>
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<p>Co-authorship analysis of authors of maize research publications under conservation agriculture between 2001 and 2023.</p>
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<p>Citation analysis of journals/sources that published articles on maize research under conservation agriculture between 2001 and 2023.</p>
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<p>Co-citation analysis of journals publishing articles on maize research under conservation agriculture between 2001 and 2023.</p>
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<p>Network visualization of words in titles, abstracts, and keywords of publications pertaining to maize research under conservation agriculture systems.</p>
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<p>Word cloud of keywords in the titles and abstracts of the screened publications (size of each word/phrase delineating its frequency/occurrence in research publications).</p>
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<p>Tree map of keywords in the titles and abstracts of the screened publications.</p>
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<p>Citation analysis of organizations involved in maize research publications under conservation agriculture between 2001 and 2023.</p>
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<p>Citation analysis of countries involved in maize research publications under conservation agriculture between 2001 and 2023.</p>
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<p>Thematic analysis of maize research under the framework of conservation agriculture.</p>
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<p>Implications and future prospects in maize research under conservation agriculture systems.</p>
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28 pages, 1655 KiB  
Review
Enhancing Olive Cultivation Resilience: Sustainable Long-Term and Short-Term Adaptation Strategies to Alleviate Climate Change Impacts
by Sandra Martins, Sandra Pereira, Lia-Tânia Dinis and Cátia Brito
Horticulturae 2024, 10(10), 1066; https://doi.org/10.3390/horticulturae10101066 - 5 Oct 2024
Viewed by 836
Abstract
Olive cultivation, an icon of Mediterranean agriculture, economy, and cultural heritage, faces significant challenges due to climate change and soil degradation. Climate projections indicate that altered precipitation patterns, rising temperatures, and increased frequency of extreme weather events will adversely affect olive tree growth, [...] Read more.
Olive cultivation, an icon of Mediterranean agriculture, economy, and cultural heritage, faces significant challenges due to climate change and soil degradation. Climate projections indicate that altered precipitation patterns, rising temperatures, and increased frequency of extreme weather events will adversely affect olive tree growth, fruit quality, and yield. This review provides a novel perspective on addressing these challenges through both long-term and short-term adaptation strategies, emphasizing innovative products, advanced technologies, and practical solutions that must work synergistically and be tailored to regional conditions. Long-term practices refer to proactive strategies for enduring climate resilience, including cover cropping, mulching, soil amendments, and breeding programs which enhance soil health, improve water retention, and increase the trees’ resilience. Short-term strategies focus on immediate impacts, offering immediate stress relief and enhanced plant physiological responses, including optimized irrigation systems, pruning management, particle coating films, biostimulants, and plant growth regulators. The review underscores the importance of aligning agricultural practices with sustainability goals and evolving environmental policies and the education of farmers and policymakers. By integrating adaptive practices and technological advancements, the olive sector can better address climate challenges, contribute to global food security, and advance environmental sustainability. Full article
(This article belongs to the Special Issue Sustainable Cultivation and Breeding of Olive Trees)
Show Figures

Figure 1

Figure 1
<p>Impact of climate change and conventional agronomic practices on olive cultivation: The interaction between climate change conditions and the frequently used conventional agronomic practices, which are inherently harmful, has detrimental effects on olive cultivation. These interactions are expected to negatively influence olive tree performance, disrupt flower and fruit set, and alter fruit growth and composition, as well as negatively impact olive oil quality and chemical composition. In turn, these effects pose significant challenges to the overall sustainability and productivity of the olive sector, leading to socioeconomic and environmental instability.</p>
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<p>Schematization of several adaptation strategies to climate change impacts, categorized into long- and short-term sustainable strategies, and implications for the olive industry.</p>
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