Search Results (2,802)

The use of Generally Recognized as Safe (GRAS)-grade microbial cell factories to produce recombinant protein-based nutritional products is a promising trend in developing food and health supplements. In this study, GRAS-grade Kluyveromyces marxianus was employed to express recombinant human heavy-chain ferritin (rhFTH), achieving a yield of 11 g/L in a 5 L fermenter, marking the highest yield reported for ferritin nanoparticle proteins to our knowledge. The rhFTH formed 12 nm spherical nanocages capable of ferroxidase activity, which involves converting Fe²⁺ to Fe³⁺ for storage. The rhFTH-containing yeast cell lysates promoted cytokine secretion (tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), and -1β (IL-1β)) and enhanced locomotion, pharyngeal pumping frequency, egg-laying capacity, and lifespan under heat and oxidative stress in the RAW264.7 mouse cell line and the C. elegans model, respectively, whereas yeast cell lysate alone had no such effects. These findings suggest that rhFTH boosts immunity, holding promise for developing ferritin-based food and nutritional products and suggesting its adjuvant potential for clinical applications of ferritin-based nanomedicine. The high-yield production of ferritin nanoparticles in K. marxianus offers a valuable source of ferritin for the development of ferritin-based products. Full article

In an age of emerging zoonoses, it is important to understand the intricate system of vectors and reservoirs, or hosts, and their relation to humans. West Nile Virus (WNV) has been detected in a myriad of nonhuman hosts. Transmission of the virus to humans is reliant on amplified seroprevalence within the host, which occurs primarily in birds. However, recent studies have found that other animal groups, including crocodilians, can obtain seroprevalence amplification to levels that make them competent hosts able to transmit WNV to mosquitoes, which can then transmit to humans. Climate change could exacerbate this transmission risk by shifting the distributions of mosquito vectors towards novel geographic ranges. Here, we use maximum entropy models to map the current and future distributions of three mosquito vector species and four crocodilian species in North America to determine the emerging risk of WNV outbreaks associated with changing climates and WNV associated with crocodilians in North America. From our models, we determined that one mosquito species in particular, Culex quinquefasciatus, will increase its distribution across the ranges of all crocodilian species in all tested climate change scenarios. This poses a potential risk to public health for people visiting and living near crocodilian farms and high-density natural crocodilian populations. Full article

Glossy buckthorn (Frangula alnus Miller) is an invasive alien plant species (IAPS) rapidly expanding in North America but is largely understudied compared to the common buckthorn (Rhamnus cathartica). Our study investigated the effects of a 27-year-old F. alnus invasion on native understory plant and saprophagous macroarthropod communities in a wet deciduous woodland in Southern Québec, Canada. We hypothesized a decreased taxonomic diversity and a change in community composition of both indicator taxa with increasing F. alnus density. The understory plant and saprophagous macroarthropod communities were characterized, respectively, through vegetation surveys and pitfall trapping across a density gradient of 43 plots invaded by F. alnus. Our results demonstrated that F. alnus did not exert a strong influence on species community composition, although the homogenization of understory plant communities was observed. Despite several decades of F. alnus invasion at our study site, the consequences on the selected indicator taxa were overall relatively small, suggesting that the magnitude of effects is variable. We suggest that further investigation at a larger scale should be performed to evaluate the effect of F. alnus on a broad diversity of indicators and understand any context dependency. Full article

Sugar kelp (Saccharina latissma) has many nutritional benefits and has been identified as a rich source of fibre, vitamins, and antioxidants. However, it is not regularly consumed in the Western world, and the sensory perception of foods containing sugar kelp must be investigated to increase acceptance in North America. This study evaluated consumers’ (n = 99) sensory perception of couscous with increasing amounts of sugar kelp (0% (control), 4%, 6%, 8%, and 10% wt/wt). Furthermore, consumers’ purchase intent, liking, and emotional response to couscous with added sugar kelp was evaluated with and without nutritional information. Sugar kelp at 6% incorporation did not impact the consumers’ liking scores (“Like Slightly” on the hedonic scale), but at 8% the consumers’ liking significantly decreased (“Neither Like nor Dislike”). The 8% and 10% levels of sugar kelp addition led to astringency, bitter, hard, brackish, fishy, and chewy attributes being perceived by the consumers. The consumers identified they preferred samples that had soft, savoury, salty, and bland flavours and disliked samples that were brackish and gritty. The nutritional information did not increase overall liking scores, purchase intent, or emotional response. However, the inclusion of sugar kelp in the couscous did lead to an increased selection of positive emotions like happy, joyful, pleasant, and enthusiastic. Overall, the consumers were interested in foods containing seaweed and believed they were nutritious. The results indicated that sugar kelp could be added to couscous up to 6% wt/wt without impacting overall liking. Full article

In light of the rapid Arctic warming and continuous reduction in Arctic Sea ice, the complex two-way Arctic–midlatitudes connection has become a focal point in recent climate research. In this paper, we review the current understanding of the interactive influence between midlatitude atmospheric variability and Arctic Sea ice or thermal conditions on interannual timescales. As sea ice diminishes, in contrast to the Arctic warming (cooling) in boreal winter (summer), Eurasia and North America have experienced anomalously cold (warm) conditions and record snowfall (rainfall), forming an opposite oscillation between the Arctic and midlatitudes. Both statistical analyses and modeling studies have demonstrated the significant impacts of autumn–winter Arctic variations on winter midlatitude cooling, cold surges, and snowfall, as well as the potential contributions of spring–summer Arctic variations to midlatitude warming, heatwaves and rainfall, particularly focusing on the role of distinct regional sea ice. The possible physical processes can be categorized into tropospheric and stratospheric pathways, with the former encompassing the swirling jet stream, horizontally propagated Rossby waves, and transient eddy–mean flow interaction, and the latter manifested as anomalous vertical propagation of quasi-stationary planetary waves and associated downward control of stratospheric anomalies. In turn, atmospheric prevailing patterns in the midlatitudes also contribute to Arctic Sea ice or thermal condition anomalies by meridional energy transport. The Arctic–midlatitudes connection fluctuates over time and is influenced by multiple factors (e.g., continuous melting of climatological sea ice, different locations and magnitudes of sea ice anomalies, internal variability, and other external forcings), undoubtedly increasing the difficulty of mechanism studies and the uncertainty surrounding predictions of midlatitude weather and climate. In conclusion, we provide a succinct summary and offer suggestions for future research. Full article

The prevalence of the highly pathogenic avian influenza virus H5N1 in wild birds that migrate all over the world has resulted in the dissemination of this virus across Asia, Europe, Africa, North and South America, the Arctic continent, and Antarctica. So far, H5N1 clade 2.3.4.4.b has reached an almost global distribution, with the exception of Australia and New Zealand for autochthonous cases. H5N1 clade 2.3.4.4.b, derived from the broad-host-range A/Goose/Guangdong/1/96 (H5N1) lineage, has evolved, adapted, and spread to species other than birds, with potential mammal-to-mammal transmission. Many public health agencies consider H5N1 influenza a real pandemic threat. In this sense, we analyzed H5N1 hemagglutinin sequences from recent outbreaks in animals, clinical samples, antigenic prototypes of candidate vaccine viruses, and licensed human vaccines for H5N1 with the aim of shedding light on the development of an H5N1 vaccine suitable for a pandemic response, should one occur in the near future. Full article

The human immunodeficiency virus type 1 (HIV-1) epidemic has been a major public health threat on a global scale since the early 1980s. Despite the introduction of combination antiretroviral therapy (cART), the incidence of new HIV-1 infections continues to rise in some regions around the world. Thus, with the continuous transmission of HIV-1 and the lack of a cure, it is imperative for molecular epidemiological studies to be performed, to monitor the infection and ultimately be able to control the spread of this virus. This work provides a comprehensive molecular epidemiological analysis of the HIV-1 infection in Cyprus, through examining 305 HIV-1 sequences collected between 9 March 2017 and 14 October 2021. Employing advanced statistical and bioinformatic techniques, the research delved deeply into understanding the transmission dynamics of the HIV-1 epidemic in Cyprus, as well as the monitoring of HIV-1’s genetic diversity and the surveillance of transmitted drug resistance. The characterization of Cyprus’s HIV-1 epidemic revealed a diverse landscape, comprising 21 HIV-1 group M pure subtypes and circulating recombinant forms (CRFs), alongside numerous uncharacterized recombinant strains. Subtypes A1 and B emerged as the most prevalent strains, followed by CRF02_AG. The findings of this study also revealed high levels of transmitted drug resistance (TDR) patterns, raising concerns for the efficacy of cART. The demographic profiles of individuals involved in HIV-1 transmission underscored the disproportionate burden borne by young to middle-aged Cypriot males, particularly those in the MSM community, who reported contracting the virus in Cyprus. An assessment of the spatiotemporal evolutionary dynamics illustrated the global interconnectedness of HIV-1 transmission networks, implicating five continents in the dissemination of strains within Cyprus: Europe, Africa, Asia, North America, and Oceania. Overall, this study advances the comprehension of the HIV-1 epidemic in Cyprus and highlights the importance of understanding HIV-1’s transmission dynamics through continuous surveillance efforts. Furthermore, this work emphasizes the critical role of state-of-the-art bioinformatics analyses in addressing the challenges posed by HIV-1 transmission globally, laying the groundwork for public health interventions aimed at curbing its spread and improving patient outcomes. Full article

Despite the numerous advantages of using natural materials, such as fast-growing, living, and earthen materials, their widespread application in the construction industry remains limited. This research presents a perception survey, which investigates stakeholders’ perceptions regarding the market, regulatory barriers, and educational barriers, exploring experiences, motivations, and attitudes toward the adoption of natural materials in construction projects. The results capture variations in current practices and identify patterns for future directions, analyzed in a comparative manner to assess two geographical regions: Europe and North America. The results show that contractor availability, a lack of professional knowledge (mostly in Europe), and cost-to-value perceptions (mostly in the USA) are key barriers to adopting natural materials. The lack of awareness among construction professionals regarding technical aspects highlights the need for targeted training, while the lack of regulatory distinction between living and earth-based materials underscores the need for harmonized policies. By elucidating stakeholders’ perspectives and identifying key challenges, this research aims to inform policymaking, industry practices, and research initiatives aimed at promoting the use of a wider lexicon of construction materials. Ultimately, this study hopes to facilitate the development of strategies to overcome scalability challenges and accelerate the transition toward their implementation in mainstream projects. Full article

This pioneering study explores the geospatial and temporal patterns of natural and human-induced disasters from 1900 to 2024, providing essential insights into their global distribution and impacts. Significant trends and disparities in disaster occurrences and their widespread consequences are revealed through the utilization of the comprehensive international EM-DAT database. The results showed a dramatic escalation in both natural and man-made (technological) disasters over the decades, with notable surges in the 1991–2000 and 2001–2010 periods. A total of 25,836 disasters were recorded worldwide, of which 69.41% were natural disasters (16,567) and 30.59% were man-made (technological) disasters (9269). The most significant increase in natural disasters occurred from 1961–1970, while man-made (technological) disasters surged substantially from 1981–1990. Seasonal trends reveal that floods peak in January and July, while storms are most frequent in June and October. Droughts and floods are the most devastating in terms of human lives, while storms and earthquakes cause the highest economic losses. The most substantial economic losses were reported during the 2001–2010 period, driven by catastrophic natural disasters in Asia and North America. Also, Asia was highlighted by our research as the most disaster-prone continent, accounting for 41.75% of global events, with 61.89% of these events being natural disasters. Oceania, despite experiencing fewer total disasters, shows a remarkable 91.51% of these as natural disasters. Africa is notable for its high incidence of man-made (technological) disasters, which constitute 43.79% of the continent’s disaster events. Europe, representing 11.96% of total disasters, exhibits a balanced distribution but tends towards natural disasters at 64.54%. Examining specific countries, China, India, and the United States emerged as the countries most frequently affected by both types of disasters. The impact of these disasters has been immense, with economic losses reaching their highest during the decade of 2010–2020, largely due to natural disasters. The human toll has been equally significant, with Asia recording the most fatalities and Africa the most injuries. Pearson’s correlation analysis identified statistically significant links between socioeconomic factors and the effects of disasters. It shows that nations with higher GDP per capita and better governance quality tend to experience fewer disasters and less severe negative consequences. These insights highlight the urgent need for tailored disaster risk management strategies that address the distinct challenges and impacts in various regions. By understanding historical disaster patterns, policymakers and stakeholders can better anticipate and manage future risks, ultimately safeguarding lives and economies. Full article

Community-supported agriculture (CSA) has emerged as a pivotal model for sustainable and humanistic agricultural practices, emphasizing the symbiotic relationship between food production, consumption, and sustainable ecosystems. Despite the growing interest, a comprehensive analysis of research themes and trends within the CSA framework remains sparse. This paper undertakes a systematic review of CSA literature from 1999 to 2023, identifying evolving research hotspots, dominant themes, and prospective directions by keyword analysis to corroborate Maslow’s hierarchy of needs theory. The research analysis location is categorized into four temporal phases, revealing a geographical expansion from North America to encompass Asia, Africa, and other continents. This expansion corroborates Maslow’s theory, illustrating a global shift from fulfilling basic physiological needs towards recognizing sustainable practices, particularly in developing regions. The results of temporal trends (5 phases) and the hotspots of keyword analysis support each other by showing a societal shift from basic sustenance to a deeper understanding of nutrition and diet. Most of the recent research keywords are grouped into the “environment” and “health and education” categories, indicating an increasing emphasis on transforming the food system and nutrition education. This review suggests conducting an integrated analysis that links the various stages of the food supply chain with the criteria outlined in the Sustainable Agriculture Matrix (SAM). It highlights that the “environment” theme is a stage of building up esteem and self-realization that needs to be unfolded in the future, given that most research on community-supported agriculture (CSA) focuses on the “economy and society” aspect and consumption stage, which burnish self-morality in the theory of Maslow. Overall, this review proposes an analysis of the relevance among different subject categories and between food supply chain stages, which reveals that the trend of research under CSA development is accorded to the theory of Maslow’s hierarchy of needs and calls for a more holistic approach to agricultural research that considers ecological, health, and social imperatives. Full article

Emerging evidence in women supports the notion that pregnancy may reset disease resistance, thereby providing protection against subsequent adverse health outcomes, but this hypothesis has not been adequately explored in domestic dogs. Cranial cruciate ligament (CCL) rupture is a degenerative orthopedic disease that frequently affects pet dogs, and its risk has been associated with disruption of the reproductive hormone axis. Our research team is conducting a lifetime cohort study of purebred Rottweilers in North America that have lived 30% longer than breed-average. Detailed medical and reproductive histories of 33 nulliparous and 32 parous Rottweilers were generated from questionnaires and review of medical records. Interviews with owners of bitches in the nulliparous group served to limit selection bias, confirming that in no instance was the reason for nulliparity based upon the owner’s suspicion that a bitch had a heightened risk for CCL rupture. The risk of CCL rupture associated with parity and other exposure variables was estimated using multivariate logistic regression. Overall, CCL rupture was diagnosed in 17 of 65 (26%) bitches. Median age at first litter and CCL rupture were 3.6 and 6.5 years, respectively. Compared to nulliparous, parous bitches had a significant 94% reduction in CCL rupture risk adjusted for duration of ovary exposure, overweight body condition, dietary pattern, habitual physical activity, and work/sport activity [OR_adjusted (95% CI) = 0.06 (0.01–0.46); (p = 0.006)]. The observed parity-associated CCL rupture risk reduction remained robust in sensitivity analysis excluding six nulliparous bitches for which decision not to breed was based on diagnosis of hip or elbow dysplasia, conditions which may be genetically linked to CCL rupture [OR_adjusted (95% CI) = 0.08 (0.01–0.58); (p = 0.01)]. This work sets the stage for replication studies in other canine populations that should begin to explore the mechanistic basis for parity-associated CCL rupture risk reduction and to pursue other non-reproductive health outcomes in bitches whose incidence or severity may be parity-sensitive. Full article

Long-term monitoring studies on the transition of different land cover units to barren areas are crucial to gain a better understanding of the potential challenges and threats that land surface ecosystems face. This study utilized the Moderate Resolution Imaging Spectroradiometer (MODIS) land cover products (MCD12C1) to conduct geospatial analysis based on the maximum extent (MaxE) concept, to assess the spatiotemporal changes in barren areas from 2001 to 2022, at global and continental scales. The MaxE area includes all the pixels across the entire period of observations where the barren land cover class was at least once present. The relative expansion or reduction of the barren areas can be directly assessed with MaxE, as any annual change observed in the barren distribution is comparable over the entire dataset. The global barren areas without any land change (UA) during this period were equivalent to 12.8% (18,875,284 km²) of the global land surface area. Interannual land cover changes to barren areas occurred in an additional area of 3,438,959 km² (2.3% of the global area). Globally, barren areas show a gradual reduction from 2001 (91.1% of MaxE) to 2012 (86.8%), followed by annual fluctuations until 2022 (88.1%). These areas were mainly interchanging between open shrublands and grasslands. A relatively high transition between barren areas and permanent snow and ice is found in Europe and North America. The results show a 3.7% decrease in global barren areas from 2001 to 2022. Areas that are predominantly not barren account for 30.6% of the transitional areas (TAs), meaning that these areas experienced short-term or very recent transitions from other land cover classes to barren. Emerging barren areas hotspots were mainly found in the Mangystau region (Kazakhstan), Tibetan plateau, northern Greenland, and the Atlas Mountains (Morocco, Tunisia). Full article

In 1837, Danish philologist Carl Christian Rafn published Antiquitates Americanæ, which introduced Americans to the Vinland sagas—medieval texts that suggest that Norse explorers “discovered” North America around the turn of the first millennium. Rafn, who saw it as his mission to promote Old Norse literature around the globe, presented some of his research in a way that would appeal to Anglo-American prejudices, particularly through the obsession with American Antiquities and the question of a pre-Columbian civilization. His conclusions and the Vinland sagas consequently entered the American racial and religious discourses. Like other discovery myths, the Vinland sagas were used by intellectuals to argue for an early white presence on the continent. Later that century, the Norse discovery was framed in religious terms as some white Americans attempted to replace the figure of Christopher Columbus with that of Leifur Eiriksson as the true discoverer of America. The ramifications of Rafn’s work and its reception can be seen in twentieth- and twenty-first-century representations of Vikings in American popular culture. Full article

Boreal and Arctic regions have warmed up to four times quicker than the rest of the planet since the 1970s. As a result, boreal and tundra ecosystems are experiencing more frequent and higher intensity extreme weather events and disturbances, such as wildfires. Yet limitations in ground and satellite data across the Arctic and boreal regions have challenged efforts to track these disturbances at regional scales. In order to effectively monitor the progression and extent of wildfires in the Arctic-boreal zone, it is essential to determine whether burned area (BA) products are accurate representations of BA. Here, we use 12 different datasets together with MODIS active fire data to determine the total yearly BA and seasonal patterns of fires in Arctic-boreal North America and Russia for the years 2001–2020. We found relatively little variability between the datasets in North America, both in terms of total BA and seasonality, with an average BA of 2.55 ± 1.24 (standard deviation) Mha/year for our analysis period, the majority (ca. 41%) of which occurs in July. In contrast, in Russia, there are large disparities between the products—GFED5 produces over four times more BA than GFED4s in southern Siberia. These disparities occur due to the different methodologies used; dNBR (differenced Normalized Burn Ratio) of short-term composites from Landsat images used alongside hotspot data was the most consistently successful in representing BA. We stress caution using GABAM in these regions, especially for the years 2001–2013, as Landsat-7 ETM+ scan lines are mistaken as burnt patches, increasing errors of commission. On the other hand, we highlight using regional products where possible, such as ABoVE-FED or ABBA in North America, and the Talucci et al. fire perimeter product in Russia, due to their detection of smaller fires which are often missed by global products. Full article

A reference material (RM), as defined by the International Vocabulary of Metrology (VIM 2012), must be homogeneous, stable, and suitable for use in measurements. Certified reference materials (CRMs) are RMs with documented property values, uncertainties, and traceability. ISO 17034:2018 outlines the requirements for RM producers, ensuring that CRMs meet standards for stability, uniformity, and reproducibility. In Europe, CE marking, from French “conformité Européenne”, which means European conformity, has been mandatory for thermal insulation products since 2002, ensuring their thermal performance is verified by accredited laboratories using RMs like IRMM440 and ERM FC440. Annually, European manufacturers produce over 200 million cubic meters of thermal insulation, necessitating thousands of thermal conductivity measurements daily to maintain CE marking compliance. Key characteristics of Reference Materials include long-term stability, thermal conductivity within specified ranges, and minimal dependence on density, thickness, and applied load. Sample thickness must conform to apparatus specifications, and homogeneity must be quantified. Reference Materials must also have appropriate dimensions, surface smoothness, and manufacturability. The Joint Research Centre (JRC) Geel has produced two Reference Materials, IRMM 440 and ERM FC 440, with specific characteristics to meet these requirements. Both are glass wool fibers with low thermal conductivity and specific density and thickness. The qualification of RMs involves inter-laboratory comparisons to ensure the accuracy and traceability of thermal conductivity measurements. The European market’s organization, including the use of Reference Materials and CE marking, has significantly improved measurement consistency and product quality. This system has led to lower uncertainties in thermal conductivity measurements compared to North America, highlighting the impact of standardized RMs on industry practices. Future needs include developing RMs with lower conductivity and increased thickness to accommodate market trends towards super insulation materials and bio-based components, enhancing energy performance calculations for buildings. This paper will present the process of defining a reference material and how it affects the uncertainty level of the calculation of building energy performance. This level depends on the characteristics of the materials used, their implementation, and external factors, such as the weather, as well as the reference material used for calibration of all European thermal conductivity measurement devices. Full article