Search Results (35,252)

Evasive Spectre attacks have used additional nop or memory delay instructions to make effective hardware performance counter based detectors with lower attack detection successful rate. Interestingly, the detection performance gets worse under different workloads. For example, the attack detection successful rate is only 59.8% for realistic applications, while it is much lower 27.52% for memory stress test. Therefore, this paper proposes a two-stage smart detector T-Smade designed for evasive Spectre attacks (e.g., evasive Spectre nop and evasive Spectre memory) under various workloads. T-Smade uses the first-stage detector to identify the type of workloads and then selects the appropriate second-stage detector, which uses four hardware performance counter events to characterize the high cache miss rate and low branch miss rate of Spectre attacks. More importantly, the second stage detector adds one dimension of reusing cache miss rate and branch miss rate to exploit the characteristics of various workloads to detect evasive Spectre attacks effectively. Furthermore, to achieve the good generalization for more unseen evasive Spectre attacks, the proposed classification detector T-Smade is trained by the raw data of Spectre attacks and non-attacks in different workloads using simple Multi-Layer Perception models. The comprehensive results demonstrate that T-Smade makes the average attack detection successful rate of evasive Spectre nop under different workload return from 27.52% to 95.42%, and that of evasive Spectre memory from 59.8% up to 100%. Full article

With the ubiquitous deployment of mobile and sensor technologies in modes of transportation, taxis have become a significant component of public transportation. However, vacant taxis represent an important waste of transportation resources. Forecasting taxi demand within a short time achieves a supply–demand balance and reduces oil emissions. Although earlier studies have forwarded highly developed machine learning- and deep learning-based models to forecast taxicab demands, these models often face significant computational expenses and cannot effectively utilize large-scale trajectory sensor data. To address these challenges, in this paper, we propose a hybrid deep learning-based model for taxi demand prediction. In particular, the Variational Mode Decomposition (VMD) algorithm is integrated along with a Bidirectional Long Short-Term Memory (BiLSTM) model to perform the prediction process. The VMD algorithm is applied to decompose time series-aware traffic features into multiple sub-modes of different frequencies. After that, the BiLSTM method is utilized to predict time series data fed with the relevant demand features. To overcome the limitation of high computational expenses, the designed model is performed on the Spark distributed platform. The performance of the proposed model is tested using a real-world dataset, and it surpasses existing state-of-the-art predictive models in terms of accuracy, efficiency, and distributed performance. These findings provide insights for enhancing the efficiency of passenger search and increasing the profit of taxicabs. Full article

Analytical instrumentation is essential for chemical analysis in many fields, including biology and chemistry, but it can be costly and inaccessible to many educational institutions because it often requires expensive and sophisticated equipment. To address this issue, there has been growing interest in developing new and accessible alternatives. In this study, we developed a low-cost and user-friendly spectrophotometric detector based on an Arduino UNO platform. This detector was coupled with a flow injection analysis system (FIA) and used to quantify the concentration of tartrazine in commercial beverages and candy samples. The proposed miniaturized detector offers an affordable and portable alternative to conventional spectrophotometers. We evaluated the performance of our detector by comparing its results with those obtained using high-performance liquid chromatography (HPLC-DAD), and the accuracy and precision were comparable. The results demonstrate the potential of the Arduino-based spectrophotometric detector as a cost-effective and accessible tool, with potential applications in food science, environmental monitoring, and other fields. Full article

This paper investigates the hydrodynamic characteristics of a rectangular otter board in different working postures by using a dynamic model. Dynamic models are mainly based on dynamic mesh techniques. The results of the dynamic model are, compared to the model test, carried out in a flume tank. Furthermore, different rotation speeds of dynamic model were analyzed. The research results are as follows: compared to flume tank results, the maximum error of the dynamic model is 23.77%. Moreover, the influence of rotation speed on the hydrodynamic board is not obvious, and 2 deg./s was chosen as the rotation speed. When the board is tilted slightly (including four working postures), its lift-to-drag ratio first increases slightly and then gradually decreases. Compared with the other three working postures, the pressure center coefficient of the board does not change significantly when it is tilted inward. When studying different working angles (including AOA and tilt angle) of the otter board, the numerical dynamic model significantly reduces repetitive setup work, making simulations more efficient. Its ability to provide continuous curves and a large volume of results offers researchers a more detailed and comprehensive understanding of the board’s hydrodynamics. Additionally, the dynamic model supports innovative fishery equipment development by allowing more accurate and continuous numerical simulations. Full article

Breast cancer is the most prevalent form of cancer in women worldwide. Triple-negative breast cancer is the most unfavorable for patients, but it is also the most sensitive to chemotherapy. Triterpene glycosides from sea cucumbers possess a high therapeutic potential as anticancer agents. This study aimed to identify the pathways triggered and regulated in MDA-MB-231 cells (triple-negative breast cancer cell line) by the glycosides cucumarioside A₀-1 (Cuc A₀-1) and djakonovioside A (Dj A), isolated from the sea cucumber Cucumaria djakonovi. Using flow cytometry, fluorescence microscopy, immunoblotting, and ELISA, the effects of micromolar concentrations of the compounds on cell cycle arrest, induction of apoptosis, the level of reactive oxygen species (ROS), mitochondrial membrane potential (Δψm), and expression of anti- and pro-apoptotic proteins were investigated. The glycosides caused cell cycle arrest, stimulated an increase in ROS production, and decreased Δψm in MDA-MB-231 cells. The depolarization of the mitochondrial membrane caused by cucumarioside A₀-1 and djakonovioside A led to an increase in the levels of APAF-1 and cytochrome C. This, in turn, resulted in the activation of caspase-9 and caspase-3 and an increase in the level of their cleaved forms. Glycosides also affected the expression of Bax and Bcl-2 proteins, which are associated with mitochondria-mediated apoptosis in MDA-MB-231 cells. These results indicate that cucumarioside A₀-1 and djakonovioside A activate the intrinsic apoptotic pathway in triple-negative breast cancer cells. Additionally, it was found that treatment with Cuc A₀-1 resulted in in vivo inhibition of tumor growth and metastasis of murine solid Ehrlich adenocarcinoma. Full article

As a propulsor with a good application prospect, the flapping foil has been a hot research topic in the past decade. Although the research results of flapping foils have been very abundant, the performance-influencing mechanism of flapping foils is still not perfect, and the research considering three-dimensional (3D) effects for engineering applications is still very limited. Based on the above considerations, a systematic and parametric analysis of a small aspect ratio flapping foil is conducted to correlate the influencing factors including angle of attack (AoA) characteristics and wake vortex on the propulsive efficiency. Three-dimensional numerical analyses of full-active and semi-active flapping foils are carried out in this paper, in which the former focuses on different heave amplitudes and pitch amplitudes, and the latter concentrates on different spring stiffnesses. The analysis covers the full range of advance coefficient, which starts around 0 and ends at a thrust drop of 0. Firstly, the influence of the maximum AoA (α_max) on the efficiency and thrust coefficient of these two kinds of flapping foils is analyzed. The results show that for the small aspect ratio flapping foil in this paper, regardless of the full-active or semi-active form, the peak efficiency as high as 75% for both generally appears around α_max = 0.2 rad, while the peak thrust coefficient of 0.5 occurs near α_max = 0.3 rad. Then, by analyzing the wake flow field, it is found that the lower efficiency of larger α_max working points is mainly due to the larger vortex dissipation loss, while the lower efficiency of smaller α_max working points is mainly due to the larger friction loss of the foil surface. Furthermore, the plumpness of different AoA curves is compared and analyzed. It was found that, unlike the results of full-active flapping foils, the shape of the AoA curve of semi-active flapping foils with different spring stiffnesses is similar, and the relationship with efficiency is not strictly corresponding. This study is expected to provide guidance on both academics and industries in relevant fields. Full article

The genotype-by-environment interaction (G × E) might have crucial impacts on the performance and fitness of agricultural species, such as Pacific whiteleg shrimp (Litopenaeus vannamei). This study explores how enhancements in management practices can counteract G × E effects on growth traits. We analyzed a selectively bred population of whiteleg shrimp spanning the latest two generations, encompassing 259 full-sib and half-sib families with 40,862 individual shrimp, measured for body weight and total length. Our analysis revealed moderate genetic correlations (0.60–0.65) between trait expressions in pond and tank environments, a significant improvement compared to earlier generations. Employing the average information-restricted maximum likelihood (REML) approach in mixed model analysis showed significant differences in heritability (h²) estimates between the two environments; however, the extent of these differences varied by trait (h² = 0.68 in pond vs. 0.37 in tank for weight, and 0.41 vs. 0.67 for length). Our results indicate that G × E effects on growth traits in this population of L. vannamei were moderate but biologically significant. Consistent with our previous estimates in this population, genetic correlations between body weight and total length remained high (close to one) in pond and tank environments. The present findings collectively demonstrate that management improvements targeting stocking density, aeration, water quality, feeds, and feeding regimes mitigated the G × E effects on two economically significant traits in this population of whiteleg shrimp. Full article

This study investigates the efficacy of a sustained-release (SR) moxidectin microsphere formulation in preventing canine heartworm infection over 18 months in Canary Hound dogs, a hunting breed common in the Canary Islands, which is a hyperendemic region. These dogs typically do not receive preventive treatments and act as reservoirs for the disease. This field study was conducted across 11 hunting kennels with 109 dogs living outdoors, none of whom were receiving heartworm prophylaxis, with Dirofilaria immitis prevalence ranging from 11.1% to 57.1% (average 36.7%). Among these, 20 clinically healthy, heartworm-negative dogs were randomly selected to receive a single subcutaneous injection of moxidectin SR (0.17 mg/kg body weight). Antigen and Knott’s tests were performed at 6, 12, 18, and 24 months. All dogs completed the study without adverse reactions and remained heartworm-negative throughout. By the end of the study, kennel heartworm prevalence ranged from 14.3% to 46.7% (average 35.4%). A single subcutaneous dose of moxidectin SR at the recommended dosage may prevent patent heartworm infection in dogs for up to 18 months in hyperendemic regions. Further studies are required to confirm these findings. Extending the efficacy period of moxidectin could improve owner compliance, particularly among those with lower animal health awareness. Full article

As an important ecological barrier and a crucial base for animal husbandry in China, the forage–livestock balance in the Three-River-Source Region (TRSR) directly impacts both the degradation and recovery of grassland. This study examines the forage–livestock balance in the TRSR over the past 13 years (2010–2022) by calculating both the theoretical and actual livestock carrying capacity, thereby providing a scientific basis for regional animal husbandry policies. Firstly, the Carnegie–Ames–Stanford Approach (CASA) model was improved to fit the specific characteristics of alpine grassland ecosystem in the TRSR. This enhanced model was subsequently used to calculate the net primary productivity (NPP) of the grassland, from which the regional grassland yield and theoretical livestock carrying capacity were derived. Secondly, the actual livestock carrying capacity was calculated and spatialized based on the number of regional year-end livestock. Finally, the livestock carrying pressure index was determined using both the theoretical and actual livestock carrying capacity. The results revealed several key findings: (1) The average grassland NPP in the TRSR was 145.44 gC/m², the average grassland yield was 922.7 kg/hm², and the average theoretical livestock carrying capacity was 0.55 SU/hm² from 2010 to 2022. Notably, all three metrics showed an increasing trend over the past 13 years, which indicates the rise in grassland vegetation activities. (2) The average actual livestock carrying capacity over the 13-year period was 0.46 SU/hm², showing a decreasing trend on the whole. The spatial distribution displayed a pattern of higher capacity in the east and lower in the west. (3) Throughout the 13 years, the TRSR generally maintained a forage–livestock balance, with an average livestock carrying pressure index of 0.96 (insufficient). However, the trend of livestock carrying pressure is on the rise, with serious overloading observed in the western part of Qumalai County and the northern part of Tongde County. Slight overloading was also noted in Zhiduo, Maduo, and Zeku Counties. Notably, Tanggulashan Town, Zhiduo, Qumalai, and Maduo Counties showed significant increases in livestock carrying pressure, while Zaduo County and the eastern regions experienced significant decreases. In conclusion, this study not only provides feasible technical methods for assessing and managing the forage–livestock balance in the TRSR but also contributes significantly to the sustainable development of the region’s grassland ecosystem and animal husbandry industry. Full article

Pancreatic ductal adenocarcinoma (PDAC) has earned a notorious reputation as one of the most formidable and deadliest malignant tumors. Within the tumor microenvironment, cancer cells have acquired the capability to maintain incessant expansion and increased proliferation in response to hypoxia via metabolic reconfiguration, leading to elevated levels of lactate within the tumor surroundings. However, there have been limited studies specifically investigating the association between hypoxia and lactic acid metabolism-related lactylation in PDAC. In this study, multiple machine learning approaches, including LASSO regression analysis, XGBoost, and Random Forest, were employed to identify hub genes and construct a prognostic risk signature. The implementation of the CERES score and single-cell analysis was used to discern a prospective therapeutic target for the management of PDAC. CCK8 assay, colony formation assays, transwell, and wound-healing assays were used to explore both the proliferation and migration of PDAC cells affected by CENPA. In conclusion, we discovered two distinct subtypes characterized by their unique hypoxia and lactylation profiles and developed a risk score to evaluate prognosis, as well as response to immunotherapy and chemotherapy, in PDAC patients. Furthermore, we indicated that CENPA may serve as a promising therapeutic target for PDAC. Full article

Ticks are obligate hematophagous ectoparasites that affect animals, and some of them transmit a wide range of pathogens including viruses, bacteria, and protozoa to both animals and humans. Several vaccines have shown immunogenicity and protective efficacy against ticks in animal models and definitive hosts. After several decades on anti-tick vaccine research, only a commercial vaccine based on a recombinant antigen is currently available. In this context, plants offer three decades of research and development on recombinant vaccine production to immunize hosts and as a delivery vehicle platform. Despite the experimental advances in plant-made vaccines to control several parasitosis and infectious diseases, no vaccine prototype has been developed against ticks. This review examines a panorama of ticks of veterinary importance, recombinant vaccine experimental developments, plant-made vaccine platforms, and perspectives on using this technology as well as the opportunities and limitations in the field of tick vaccine research. Full article

Background: Cholangiocarcinoma (CCA) is a highly aggressive cancer of the bile ducts with a poor prognosis and limited diagnostic markers. This study aims to investigate the potential of miR-18a and miR-532 as biomarkers for CCA by exploring their correlations with clinical parameters and traditional tumor markers such as CA19.9, CEA, and AFP. Methods: This study involved a cohort of patients diagnosed with CCA. Serum levels of miR-18a and miR-532 were measured and analyzed in relation to various clinical parameters, including age, tumor markers, and histological features. Results: Serum levels of miR-18a and miR-532 were upregulated in patients with extrahepatic cholangiocarcinoma (eCCA) compared to healthy controls (p < 0.05). MiR-18a and miR-532 levels were correlated with each other (p = 0.011, Spearman’s rho = 0.482) but showed no significant correlation with age or traditional tumor markers (CA19.9, CEA, AFP). No significant differences in miR-18a and miR-532 levels were observed concerning tumor localization or histological grading. For predicting tumor resectability, miR-532 at a cut-off point of 2.12 showed a sensitivity of 72.73%, specificity of 81.25%, and an AUC of 71.3%, while miR-18a, at a cut-off of 1.83, had a sensitivity of 63.64%, specificity of 75%, and an AUC of 59.7%. ROC curve analysis suggested moderate diagnostic potential for miR-18a and miR-532, with AUC values of 0.64 and 0.689, respectively. Conclusions: Although miR-18a and miR-532 showed significant upregulation in eCCA patients compared to healthy controls, they did not demonstrate significant associations with key clinical parameters, limiting their effectiveness as standalone diagnostic biomarkers. Further research involving larger, multi-center cohorts and additional molecular markers is necessary to validate these findings and explore the broader diagnostic potential of miRNAs in CCA. Full article

The safety of sea bass is critical for the global food trade. This study evaluated the effectiveness of atmospheric cold plasma in reducing food safety risks posed by Salmonella Enteritidis and Listeria monocytogenes, which can contaminate sea bass post harvest. Cold plasma was applied to inoculated sea bass for 2 to 18 min, achieving a maximum reduction of 1.43 log CFU/g for S. Enteritidis and 0.80 log CFU/g for L. monocytogenes at 18 min. Longer treatments resulted in greater reductions; however, odor and taste quality declined to a below average quality in samples treated for 12 min or longer. Plasma treatment did not significantly alter the color, texture, or water activity (aw) of the fish. Higher levels of thiobarbituric acid reactive substances (TBARSs) were observed with increased exposure times. Cold plasma was also tested in vitro on S. Enteritidis and L. monocytogenes on agar surfaces. A 4 min treatment eliminated the initial loads of S. Enteritidis (2.71 log CFU) and L. monocytogenes (2.98 log CFU). The findings highlight the potential of cold plasma in enhancing the safety of naturally contaminated fish. Cold plasma represents a promising technology for improving food safety in the global fish trade and continues to be a significant area of research in food science. Full article

Metal oxide nanoparticles (MONs) are particles with at least one dimension in the nanoscale range (1–100 nm). Their unique properties, significantly different from their bulk counterparts, make them promising materials for a wide range of applications in fields such as medicine, electronics, catalysis, environmental remediation, and energy storage. The precise control of MONs’ properties, including size, shape, composition, crystallinity, and surface chemistry, is significant for optimizing their performance. This study aims to investigate the characteristics of synthesis methods of MONs. Correlation between synthesis parameters and properties highlights that creating nanomaterials with defined and controlled dimensions is a complex task that requires a deep understanding of various factors. Also, this study presents a model with adaptive parameters for synthesis conditions to acquire desired nanometric scale for particles size, which represents an essential task. Full article

The global prevalence of aflatoxin B1 (AFB1) and zearalenone (ZEN) contamination in food and feed poses a serious health risk to humans and animals. Recently, enzymatic detoxification has received increasing attention, yet most enzymes are limited to degrading only one type of mycotoxin, and free enzymes often exhibit reduced stability and activity, limiting their practicality in real-world applications. In this study, the laccase CotA gene from ZEN/AFB1-degrading Bacillus subtilis ZJ-2019-1 was cloned and successfully expressed in Escherichia coli BL21, achieving a protein yield of 7.0 mg/g. The recombinant CotA (rCotA) completely degraded AFB1 and ZEN, with optimal activity at 70 °C and pH 7.0. After rCotA treatment, neither AFB1 nor ZEN showed significantly cytotoxicity to mouse macrophage cell lines. Additionally, the AFB1/ZEN degradation efficiency of rCotA was significantly enhanced by five natural redox mediators: acetosyringone, syringaldehyde, vanillin, matrine, and sophoridin. Among them, the acetosyringone-rCotA was the most effective mediator system, which could completely degrade 10 μg of AFB1 and ZEN within 1 h. Furthermore, the chitosan-immobilized rCotA system exhibited higher degradation activity than free rCotA. The immobilized rCotA degraded 27.95% of ZEN and 41.37% of AFB1 in contaminated maize meal within 12 h, and it still maintained more than 40% activity after 12 reuse cycles. These results suggest that media-assisted or immobilized enzyme systems not only boost degradation efficiency but also demonstrate remarkable reusability, offering promising strategies to enhance the degradation efficiency of rCotA for mycotoxin detoxification. Full article