Search Results (42,159)

Biofertilizers are environmentally friendly compounds that can enhance plant growth and substitute for chemically synthesized products. In this research, a new strain of the bacterium Bacillus velezensis, designated JZ, was isolated from the roots of strawberry plants and exhibited potent antagonistic properties against Bacillus altitudinis m-1, a pathogen responsible for leaf spot disease in strawberry. The fermentation broth of JZ exerted an inhibition rate of 47.43% against this pathogen. Using an optimized acid precipitation method, crude extracts of lipopeptides from the JZ fermentation broth were obtained. The crude extract of B. velezensis JZ fermentation broth did not significantly disrupt the cell permeability of B. altitudinis m-1, whereas it notably reduced the Ca²⁺-ATPase activity on the cell membrane and markedly elevated the intracellular reactive oxygen species (ROS) concentration. To identify the active compounds within the crude extract, QTOF-MS/MS was employed, revealing four antimicrobial compounds: fengycin, iturin, surfactin, and a polyene antibiotic known as bacillaene. The strain JZ also produced various plant-growth-promoting substances, such as protease, IAA, and siderophore, which assists plants to survive under pathogen infection. These findings suggest that the JZ strain holds significant potential as a biological control agent against B. altitudinis, providing a promising avenue for the management of plant bacterial disease. Full article

Existing skeleton-based action recognition methods face the challenges of insufficient spatiotemporal feature mining and a low efficiency of information transmission. To solve these problems, this paper proposes a model called the Spatial–Temporal Attention Residual Network for 3D human action recognition (3D-STARNET). This model significantly improves the performance of action recognition through the following three main innovations: (1) the conversion from skeleton points to heat maps. Using Gaussian transform to convert skeleton point data into heat maps effectively reduces the model’s strong dependence on the original skeleton point data and enhances the stability and robustness of the data; (2) a spatiotemporal attention mechanism (STA). A novel spatiotemporal attention mechanism is proposed, focusing on the extraction of key frames and key areas within frames, which significantly enhances the model’s ability to identify behavioral patterns; (3) a multi-stage residual structure (MS-Residual). The introduction of a multi-stage residual structure improves the efficiency of data transmission in the network, solves the gradient vanishing problem in deep networks, and helps to improve the recognition efficiency of the model. Experimental results on the NTU-RGBD120 dataset show that 3D-STARNET has significantly improved the accuracy of action recognition, and the top1 accuracy of the overall network reached 96.74%. This method not only solves the robustness shortcomings of existing methods, but also improves the ability to capture spatiotemporal features, providing an efficient and widely applicable solution for action recognition based on skeletal data. Full article

Graviera Naxos, a renowned cheese with Protected Designation of Origin status, is crafted from a blend of cow, goat, and sheep milk. This study focused on assessing the Sr isotopic and multi-elemental composition of both the processed cheese and its ingredients, as well as the environmental context of Naxos Island, including samples of milk, water, soil, and feed. The objective was to delineate the geochemical signature of Graviera Naxos cheese and to explore the utility of Sr isotopes as indicators of geographic origin. The ⁸⁷Sr/⁸⁶Sr values for Graviera Naxos samples ranged from 0.70891 to 0.70952, indicating a relatively narrow range. However, the Sr isotopic signature of milk, heavily influenced by the feed, which originates from geologically distinct areas, does not always accurately reflect the local breeding environment. Multi-elemental analysis revealed variations in milk composition based on type and season; yet, no notable differences were found between raw and pasteurized milk. A mixing model evaluating the contributions of milk, sea salt, and rennet to the cheese’s Sr isotopic signature suggested a significant average contribution of 73.1% from sea salt. This study highlights the complexities of linking dairy products with their geographical origins and emphasizes the need for sophisticated geochemical authentication methods. Full article

Due to the material properties of current ski face masks for adolescents, moisture in exhaled air can become trapped within the material fibers and freeze, leading to potential issues such as breathing difficulties and increased risk of facial frostbite after prolonged skiing. This paper proposes a research approach combining computational fluid dynamics (CFD) and ergonomics to address these issues and enhance the comfort of adolescent skiers. We developed head and face mask models based on the head dimensions of 15–17-year-old males. For enclosed cavities, ensuring the smooth expulsion of exhaled air to prevent re-inhalation is the primary challenge. Through fluid simulation of airflow characteristics within the cavity, we evaluated three different inlet configurations. The results indicate that the location of the air inlets significantly affects the airflow characteristics within the cavity. The side inlet design (type II) showed an average face temperature of 35.35 °C, a 38.5% reduction in average CO₂ concentration within the cavity, and a smaller vortex area compared to the other two inlet configurations. Although the difference in airflow velocity within the cavity among the three configurations was minimal, the average exit velocity differed by up to 0.11 m/s. Thus, we conclude that the side inlet configuration offers minimal obstruction to airflow circulation and better thermal insulation when used in the design of fully enclosed helmets. This enhances the safety and comfort of adolescent wearers during physical activities in cold environments. Through this study, we aim to further promote the development of skiing education, enhance the overall quality of adolescents’ skiing, and thus provide them with more opportunities for the future. Full article

Recent advancements in proteomics have enhanced our understanding of clear cell renal cell carcinoma (CCRCC). Utilizing a combination of liquid chromatography-tandem mass spectrometry (LC-MS/MS) followed by immunohistochemical validation, we investigated the expression levels of UCHL1, PAK4, and SNRNP200 in high-grade CCRCC samples. Our analysis also integrated Reactome pathway enrichment to elucidate the roles of these proteins in cancer-related pathways. Our results revealed significant upregulation of UCHL1 and SNRNP200 and downregulation of PAK4 in high-grade CCRCC tissues compared to non-cancerous tissues. UCHL1, a member of the ubiquitin carboxy-terminal hydrolase family, showed variable expression across different tissues and was notably involved in the Akt signaling pathway, which plays a critical role in cellular survival in various cancers. SNRNP200, a key component of the RNA splicing machinery, was found to be essential for proper cell cycle progression and possibly linked to autosomal dominant retinitis pigmentosa. PAK4’s role was noted as critical in RCC cell proliferation and invasion and its expression correlated significantly with poor progression-free survival in CCRCC. Additionally, the expression patterns of these proteins suggested potential as prognostic markers for aggressive disease phenotypes. This study confirms the upregulation of UCHL1, SNRNP200, and PAK4 as significant factors in the progression of high-grade CCRCC, linking their enhanced expression to poor clinical outcomes. These findings propose these proteins as potential prognostic markers and therapeutic targets in CCRCC, offering novel insights into the molecular landscape of this malignancy and highlighting the importance of targeted therapeutic interventions. Full article

To have a deeper understanding on the moisture migration patterns and microstructural changes of Green Sichuan Pepper during the hot-air drying process, the low-field nuclear magnetic resonance (LF-NMR) technology and scanning electron microscopy (SEM) methodology were adopted to analyze the moisture distribution, migration patterns and microscopic structural changes under different drying temperatures (45, 55 and 65 °C). The LF-NMR scanning results showed that the internal moisture of the Green Sichuan Pepper mainly includes bound water, immobilized water and free water, which can be respectively symbolized by the relaxation time ranges of T₂₁ (0.1–10 ms), T₂₂ (10–500 ms) and T₂₃ (500–10,000 ms). The immobilized water accounts for 83.72% of the internal water, resulting in the significant drying difficulty of Green Sichuan Pepper. During the drying process, the content of immobilized water and free water exhibited a decreasing trend, while the bound moisture content initially increased and then decreased. In addition, the LF-NMR analysis showed that the parameters peak area A₂ demonstrated a high correlation with the moisture content of Green Sichuan Pepper, enabling the prediction of moisture content changes during the drying process. Additionally, the SEM results showed that the pore degree and pore density on the pericarp surface of Green Sichuan Pepper perform significant changes during the drying process, which might be a good explanation for revealing some commonly recognized drying phenomena on Green Sichuan Pepper hot-air drying. In summary, the findings presented in the present work provide some new insights into the moisture distribution, migration patterns and microstructural changes of Green Sichuan Pepper, which can offer theoretical guidance for optimizing the drying process of Green Sichuan Pepper. Full article

Landslide risk management contributes to the sustainable development of the region. Understanding the spatial controls on the distribution of landslides triggered by earthquakes (EqTLs) is difficult in terms of the prediction and risk assessment of EqTLs. In this study, landslides are regarded as a spatial point pattern to test the controls on the spatial distribution of landslides and model the landslide density prediction. Taking more than 190,000 landslides triggered by the 2008 Wenchuan Ms 8.0 earthquake (WcEqTLs) as the research object, the relative density estimation, Kolmogorov–Smirnov testing based on cumulative distribution, receiver operating characteristic curve (ROC) analysis, and Poisson density modeling are comprehensively applied to quantitatively determine and discuss the different control effects of seven factors representing earthquakes, geology, and topography. The distance to the surface ruptures (dSR) and the distance to the epicenter (dEp) show significant and strong control effects, which are far stronger than the other five factors. Using only the dSR, dEp, engineering geological rock group (Eg), and the range, a particularly effective Poisson model of landslide density is constructed, whose area under the ROC (AUC) reaches 0.9244 and whose very high-density (VHD) zones can contain 50% of landslides and only comprise 3.9% of the study areas. This research not only deepens our understanding of the spatial distribution of WcEqTLs but also provides new technical methods for such investigation and analysis. Full article

The aim of the current investigation was to examine the locomotor performance profile of elite ladies Gaelic football (LGF) players in relation to playing position and quarters of play. Thirty (n = 30) elite LGF players (age: 24 ± 4 years, height: 169 ± 5 cm, body mass: 61 ± 4 kg) were monitored using 10 Hz GPS technology (Playertek; Catapult Sports; Australia) resulting in 145 individual samples collected over 18 competitive matches across the 2021 and 2022 LGF seasons. Locomotor performance was determined across the following variables: total distance covered (TD, m), relative distance (m·min⁻¹), high-speed running (HSR, ≥ 4.4 m·s⁻¹), very high-speed running (VHSR, ≥ 5.5 m·s⁻¹), relative HSR (RHSR; m·min⁻¹), peak velocity (m·s⁻¹), percentage peak velocity (%PeakV), accelerations (n; ≥ 3 m·s⁻²), and decelerations (n; ≤ −3 m·s⁻²). Data were classified based on playing position and quarter of play. The greatest TD was covered by half-backs, midfielders, and half-forwards, with these positions covering significantly greater distances than full-backs (p < 0.05). Similarly, half-backs, midfielders, and half-forwards covered the greatest high-speed distance (HSR). When running performance was analysed across quarters, a significant position by quarter interaction was observed for all running performance variables, except peak velocity and percentage peak velocity. A consistent trend for reduced locomotor performance was evident in the second and fourth quarters across all positional lines. The current data provide coaches with the position-specific locomotor requirements of LGF match-play, which can inform the design of training content for LGF players, along with match-day strategies. Given the reduction in locomotor performance observed across the match, performance staff may consider the use of nutritional interventions, rewarm-up strategies, or specific substitution policies to mitigate the decrement in locomotor performance observed across match-play. Full article

Psoriasis is an increasingly common chronic immune-mediated skin disease recognized for its systemic effects that extend beyond the skin and include various cardiovascular diseases, neurological diseases, type 2 diabetes, and metabolic syndrome. This study aimed to explore the complex relationship between psoriasis and metabolic syndrome by analyzing clinical, biochemical, and immunological parameters in patients with psoriasis alone and in patients combining psoriasis and metabolic syndrome. A total of 150 patients were enrolled, 76 with psoriasis only (PSO) and 74 with psoriasis and metabolic syndrome (PSO–MS). Data collected included anthropometric measurements, blood tests, and inflammatory markers. Statistical analysis was performed using the independent t-test, Mann–Whitney U test, Kruskal–Wallis test, and chi-square test to compare the two groups. Patients in the PSO–MS group had a significantly higher body weight, abdominal circumference, BMI, and inflammatory markers compared to patients with PSO. In addition, increased levels of IL-17A, cholesterol, triglycerides, and glucose were observed in the PSO–MS group. This study highlights the increased metabolic risk and exacerbated systemic inflammation associated with the coexistence of psoriasis and metabolic syndrome. These findings demonstrate the need for a comprehensive therapeutic approach and early intervention to manage metabolic complications in patients with psoriasis and metabolic syndrome. Full article

Reproduction is classically controlled by gonadotropin-releasing hormone (GnRH-I) and its receptor (GnRHR-I) within the brain. In pigs, a second form (GnRH-II) and its specific receptor (GnRHR-II) are also produced, with greater abundance in peripheral vs. central reproductive tissues. The binding of GnRH-II to GnRHR-II has been implicated in the autocrine/paracrine regulation of gonadal steroidogenesis rather than gonadotropin secretion. Blood samples were collected from transgenic gilts, with the ubiquitous knockdown of GnRHR-II (GnRHR-II KD; n = 8) and littermate controls (n = 7) at the onset of estrus (follicular) and 10 days later (luteal); serum concentrations of 16 steroid hormones were quantified by high-performance liquid chromatography tandem mass spectrometry (HPLC-MS/MS). Upon euthanasia, ovarian weight (OWT), ovulation rate (OR), and the weight of each excised Corpus luteum (CLWT) were recorded; HPLC-MS/MS was performed on CL homogenates. During the luteal phase, serum progesterone concentration was reduced by 18% in GnRHR-II KD versus control gilts (p = 0.0329). Age and weight at puberty, estrous cycle length, and OWT were similar between lines (p > 0.05). Interestingly, OR was reduced (p = 0.0123), and total CLWT tended to be reduced (p = 0.0958) in GnRHR-II KD compared with control females. Luteal cells in CL sections from GnRHR-II KD gilts were hypotrophic (p < 0.0001). Therefore, GnRH-II and its receptor may help regulate OR, CL development, and progesterone production in gilts. Full article

Tendon disorders often result in decreased muscle function and atrophy. Pulsed Electromagnetic Fields (PEMFs) have shown potential in improving tendon fiber structure and muscle recovery. However, the molecular effects of PEMF therapy on skeletal muscle, beyond conventional metrics like MRI or markers of muscle decline, remain largely unexplored. This study investigates the metabolic and structural changes in PEMF-treated muscle tissue using proteomics in a rat model of Achilles tendinopathy induced by collagenase. Sprague Dawley rats were unilaterally induced for tendinopathy with type I collagenase injection and exposed to PEMFs for 8 h/day. Gastrocnemius extracts from untreated or PEMF-treated rats were analyzed with LC-MS/MS, and proteomics differential analysis was conducted through label-free quantitation. PEMF-treated animals exhibited decreased glycolysis and increased LDHB expression, enhancing NAD signaling and ATP production, which boosted respiratory chain activity and fatty acid beta-oxidation. Antioxidant protein levels increased, controlling ROS production. PEMF therapy restored PGC1alpha and YAP levels, decreased by tendinopathy. Additionally, myosins regulating slow-twitch fibers and proteins involved in fiber alignment and force transmission increased, supporting muscle recovery and contractile function. Our findings show that PEMF treatment modulates NAD signaling and oxidative phosphorylation, aiding muscle recovery through the upregulation of YAP and PGC1alpha and increasing slow myosin isoforms, thus speeding up physiological recovery. Full article

Port wine vinegar, a product of the esteemed Port wine, is renowned for its intricate blend of flavors and aromas, a result of complex microbial interactions. This study delves into the fascinating world of yeast and acetic acid bacteria (AAB) interactions during fermentation, which significantly influence the vinegar’s chemical composition and sensory properties. We specifically investigate the role of yeasts in fermenting sugars into ethanol, a process that AAB then converts into acetic acid. The impact of these interactions on the production of secondary metabolites, such as gluconic acid, ketones, aldehydes, and esters, which contribute to the vinegar’s unique sensory profile, is thoroughly examined. Advanced analytical techniques, including GC-MS and e-nose technology, alongside sensory evaluation, are employed to assess these effects. The research underscores the significance of ethanol tolerance in AAB and other production challenges in determining vinegar quality and underscores the importance of optimizing fermentation conditions and sustainable practices. The findings of this study underscore the importance of strain interactions and production techniques, which can significantly enhance the quality and market appeal of Port wine vinegar, providing valuable insights for the industry. This review also identifies exciting and critical areas for future research, inspiring further exploration and proposing strategies for advancing production and application in culinary, health, and industrial contexts. Full article

Pituitary adenylate cyclase-activating polypeptide (PACAP) and vasoactive intestinal peptide (VIP) are two neuroprotective and anti-inflammatory molecules of the central nervous system (CNS). Both bind to three G protein-coupled receptors, namely PAC1, VPAC1 and VPAC2, to elicit their beneficial effects in various CNS diseases, including multiple sclerosis (MS). In this study, we assessed the expression and distribution of PACAP/VIP receptors in the normal-appearing white matter (NAWM) of MS donors with a clinical history of either relapsing–remitting MS (RRMS), primary MS (PPMS), secondary progressive MS (SPMS) or in aged-matched non-MS controls. Gene expression studies revealed MS-subtype specific changes in PACAP and VIP and in the receptors’ levels in the NAWM, which were partly corroborated by immunohistochemical analyses. Most PAC1 immunoreactivity was restricted to myelin-producing cells, whereas VPAC1 reactivity was diffused within the neuropil and in axonal bundles, and VPAC2 in small vessel walls. Within and around lesioned areas, glial cells were the predominant populations showing reactivity for the different PACAP/VIP receptors, with distinctive patterns across MS subtypes. Together, these data identify the differential expression patterns of PACAP/VIP receptors among the different MS clinical entities. These results may offer opportunities for the development of personalized therapeutic approaches to treating MS and/or other demyelinating disorders. Full article

The arid area of Northwest China belongs to the rain-fed agricultural area of the Loess Plateau, and water resources have become one of the important factors limiting agricultural development in this area. This study employed the AquaCrop model to predict the yield advantages and environmental adaptability of maize in Dingxi City from 2016 to 2020 under two cultivation practices: ridge tillage (100% film coverage with double ridge-furrow planting) and flat planting (81.8% film coverage with wide-film planting). The numerical simulation of the tillage and fertilization process of the double-ridge seedbed was carried out by EDEM, and the key components were tested by the Box–Behnken center combination test design principle to obtain the optimal parameter combination. The results showed that ridge planting was more suitable for agricultural planting in rain-fed arid areas in Northwest China. The simulation analysis of ridging and fertilization showed that the forward speed of the combined machine was 0.50 m/s, the rotation speed of the trough wheel of the fertilizer discharger was 39 rmp, and the rotary tillage depth was 150 mm. The qualified rate of seedbed tillage was 93.6%, and the qualified rate of fertilization was 92.1%. The research shows that the whole-film double ridge-furrow sowing technology of maize is more suitable for the rain-fed agricultural area in the arid area of Northwest China. The simulation results of the ridging fertilization device are consistent with the field experiment results. The research results provide a certain technical reference for the optimization of the whole-film double ridge-furrow sowing technology. Full article

Despite advances in systemic chemotherapy, patients with gastric cancer (GC) and peritoneal metastases (PMs) continue to have poor prognoses. Intraperitoneal (IP) administration of Paclitaxel (PTX) combined with systemic chemotherapy shows promise in treating PMs from GC. However, methods of drug administration need to be optimized to maximize efficacy. In this study, we utilized a mouse model with PMs derived from a human GC cell line, administering PTX either IP or intravenously (IV), and Carboplatin (CBDCA) IV 0, 1, and 4 days after PTX administration. The PMs were resected 30 min later, and concentrations of PTX and CBDCA in resected tumors were measured using liquid chromatography–tandem mass spectrometry (LC-MS/MS). Results indicated that PTX concentrations were higher with IP administration than with IV administration, with significant differences observed on days 0 and 1. CBDCA concentrations 4 days post-IP PTX administration were higher than with simultaneous IV PTX administration. These findings suggest that IP PTX administration enhances CBDCA concentration in peritoneal tumors. Therefore, sequential IV administration of anti-cancer drugs appears more effective than simultaneous administration with IP PTX, a strategy that may improve prognoses for patients with PMs. Full article