Search Results (1,065)

On 1 January 2001, the IEC 61000-3-2 regulation became effective. Since then, mitigating current harmonics has been essential to ensure that electronic equipment connected to single-phase power distribution lines conforms to electromagnetic compatibility directives. Today, high-quality rectification, commonly known as power factor correction (PFC), is a well-established technique widely adopted by the industry for powering various devices from the ac line. The topic has been studied by academia and industry since the mid-1980s; thus, an enormous amount of research has been published and countless solutions have been proposed since then. However, only a few of those solutions have encountered wide industrial usage. So, it is not the authors’ intention to provide a comprehensive review, but to take stock of the most used PFC techniques from an industry perspective. This paper will review the power factor theory with non-sinusoidal currents, the practical and regulatory aspects of using PFC, and the most common industry solutions for power factor correction in equipment operated from the single-phase, public, low-voltage supply system, with a special focus on boost PFC pre-regulators, their control methods, design procedures, and issues. Full article

Side-channel analysis is a type of cryptanalysis that utilizes the physical leakage of a cryptographic device. An adversary exploits the relationship between a physical leakage and the secret intermediate value of an encryption algorithm. In order to prevent side-channel analysis, the masking method was proposed. Several masking methods of the ISO/IEC 18033-3 standard encryption algorithm SEED have been proposed, as the Korean financial IC (integrated circuit) card standard (CFIP.ST.FINIC-01-2021) mandates using a robust implementation of SEED as an encryption algorithm against side-channel analyses. However, vulnerabilities were reported, except for with only one masking method. This study proposes the first-order vulnerability of that masking method. That is, an adversary is able to perform a side-channel analysis with the same complexity as an unprotected implementation. In order to fix this vulnerability, we revise the masking method with negligible additional overhead. Its vulnerability and security are theoretically verified and experimentally demonstrated. The round key of the existing masking method is revealed with only 210 power consumption traces, while that of the proposed masking method is not disclosed with 10,000 traces. Full article

Zika virus (ZIKV) is an arbovirus with maternal, sexual, and TORCH-related transmission capabilities. After 2015, Brazil had the highest number of ZIVK-infected pregnant women who lost their babies or delivered them with Congenital ZIKV Syndrome (CZS). ZIKV triggers an immune defense in the placenta. This immune response counts with the participation of interleukins and transcription factors. Additionally, it has the potential involvement of human endogenous retroviruses (HERVS). Interleukins are immune response regulators that aid immune tolerance and support syncytial structure development in the placenta, where syncytin receptors facilitate vital cell-to-cell fusion events. HERVs are remnants of ancient viral infections that integrate into the genome and produce syncytin proteins crucial for placental development. Since ZIKV can infect trophoblast cells, we analyzed the relationship between ZIKV infection, HERV, interleukin, and transcription factor modulations in the placenta. To investigate the impact of ZIKV on trophoblast cells, we examined two cell types (BeWo and HTR8) infected with ZIKV-MR766 (African) and ZIKV-IEC-Paraíba (Asian–Brazilian) using Taqman and RT2 Profiler PCR Array assays. Our results indicate that early ZIKV infection (24–72 h) does not induce differential interleukins, transcription factors, and HERV expression. However, we show that the expression of a few of these host defense genes appears to be linked independently of ZIKV infection. Future studies involving additional trophoblastic cell lineages and extended infection timelines will illuminate the dynamic interplay between ZIKV, HERVs, interleukins, and transcription factors in the placenta. Full article

Newborn lambs are susceptible to pathogenic bacterial infections leading to enteritis, which affects their growth and development and causes losses in sheep production. It has been reported that N6-methyladenosine (m6A) is closely related to innate immunity, but the effect of m6A on sheep small intestinal epithelial cells (IECs) and the mechanism involved have not been elucidated. Here, we investigated the effects of m6A on lipopolysaccharide (LPS)-induced inflammatory responses, apoptosis and oxidative stress in primary sheep IECs. First, the extracted IECs were identified by immunofluorescence using the epithelial cell signature protein cytokeratin 18 (CK18), and the cellular activity of IECs induced by different concentrations of LPS was determined by the CCK8 assay. Meanwhile, LPS could induce the upregulation of mRNA and protein levels of IECs cytokines IL1β, IL6 and TNFα and the apoptosis marker genes caspase-3, caspase-9, Bax, and apoptosis rate, reactive oxygen species (ROS) levels and mRNA levels of CAT, Mn-SOD and CuZn-SOD, and METTL3 were found to be upregulated during induction. It was hypothesized that METTL3 may have a potential effect on the induction of IECs by LPS. Overexpression and knockdown of METTL3 in IECs revealed that a low-level expression of METTL3 could reduce the inflammatory response, apoptosis and ROS levels in LPS-induced IECs to some extent. The results suggest that METTL3 may be a genetic marker for potential resistance to cellular damage. Full article

The active power losses are dependent on the flowing electric power value through overhead and cable lines. The current flow through the conductor causes negative phenomena to occur, such as released heat. The source of the current harmonics is the non-linear loads. Hence, the skin effect occurs, and the current carrying capacity of cables is reduced. This results in the increase in and uneven distribution of the temperature inside the conductor. This paper presents a comparison of the temperature distribution inside a power cable for an ideal 50 Hz sine wave and highly distorted current $(TH{D}_I=41\%)$. The calculated active power losses for the IEC 60287-1-1:2006+A1:2014 standard and the method described in the literature were used as a basis for further calculations. The obtained results revealed the problem of the uneven distribution of the conductor temperature. Considering the skin effect, increasing the temperature in the outer layers leads to severe damage and faster insulation aging. The abovementioned phenomenon is a decrease in the permissible load capacity of the conductor. The table given in the IEC 60364-5-52 standard summarizes the percentage contribution of the third harmonic to the current waveform. For percentages between 15% and 33%, the current carrying capacity is reduced by up to 86% of the full-load current rating. In addition, consideration of thermal conditions forces the use of cables with larger cross-sections. This leads to their non-optimal use and makes the investment more expensive from an economic point of view. Full article

From time to time, a series of unpredictable and conflicting contingencies can lead to angular instability of the power system and even blackouts if not adequately handled by an out-of-step (OOS) protection system. The key contribution of this research work, to the theory of out-of-step protection, is the identification and isolation after a given disruption of many unstable swings. This paper presents a proposed method to avoid false operation for distance function by out-of-step blocking to improve the system stability by using optimally placed PMUs for the fast detection of system analogue quantities. The studies were performed on a modified Eskom transmission network in the Western Cape with 765 kV and 400 kV voltage levels. The aim is to investigate the IEC 61850-90-5 standard for predictive dynamic stability maintaining systems using PMUs for out-of-step conditions of synchronous generators. The power system modelling and simulation are performed in the RSCAD-FX for the proposed multi-area power system network. An experimental lab-scale implementation is built to test the proposed out-of-step algorithm in a real-time digital simulator. Software-based PMU is incorporated to test and validate the IEC 61850-90-5 standard sampled values. Simulation and experimental results are presented. Full article

The mechanism underlying intestinal fibrosis, the main complication of inflammatory bowel disease (IBD), is not yet fully understood, and there is no therapy to prevent or reverse fibrosis. We evaluated, in in vitro cellular models, the ability of different classes of drugs currently used in IBD to counteract two pivotal processes of intestinal fibrosis, the differentiation of intestinal fibroblasts to activated myofibroblasts using CCD-18Co cells, and the epithelial-to-mesenchymal transition (EMT) of intestinal epithelial cells using Caco-2 cells (IEC), both being processes induced by transforming growth factor-β1 (TGF-β1). The drugs tested included mesalamine, azathioprine, methotrexate, prednisone, methylprednisolone, budesonide, infliximab, and adalimumab. The expression of fibrosis and EMT markers (collagen-I, α-SMA, pSmad2/3, occludin) was assessed by Western blot analysis and by immunofluorescence. Of the drugs used, only prednisone, methylprednisolone, budesonide, and adalimumab were able to antagonize the pro-fibrotic effects induced by TGF-β1 on CCD-18Co cells, reducing the fibrosis marker expression. Methylprednisolone, budesonide, and adalimumab were also able to significantly counteract the TGF-β1-induced EMT process on Caco-2 IEC by increasing occludin and decreasing α-SMA expression. This is the first study that evaluates, using in vitro cellular models, the direct antifibrotic effects of drugs currently used in IBD, highlighting which drugs have potential antifibrotic effects. Full article

Power quality measurements are essential to monitor, analyse and control the operation of smart grids within power systems. This work aims to develop and calibrate a PQ network analyser. As the penetration of non-linear loads connected to power systems is increasing every day, it is essential to measure power quality. In this sense, a power quality (PQ) analyser is based on the high-speed sampling of electrical signals in single-phase and three-phase electrical installations, which are available in real time for analysis using wireless Wi-Fi (Wireless-Fidelity) networks. The PQAE (Power Quality Analyser Embedded) power quality analyser has met the calibration standards for Class A devices from IEC 61000-4-30, IEC 61000-4-7 and IEC 62586-2. In this paper, a complete guide to the tests included in this standard has been provided. The Fast Fourier Transform (FFT) obtains the harmonic components from the measured signals and the window functions used reduce spectral leakage. The window size depends on the fundamental frequency of, intensity of and changes in the signal. Harmonic measurements from the 2nd to 50th harmonics for each phase of the voltage and each phase and neutral of the current have been performed, using the Fast Fourier transform algorithm with various window functions and their comparisons. PQAE is developed on an open-source platform that allows you to adapt its programming to the measurement needs of the users. Full article

Co-formulated antibodies can bring clinical benefits to patients by combining two or more antibodies in a single dosage form. However, the quality analysis of co-formulated antibodies raises additional challenges, compared to individual antibodies, due to the need for accurate analysis of multiple antibodies in one solution. It is extremely difficult to effectively separate the charge variants of the two co-formulated antibodies using one ion exchange chromatography (IEC) method because of their similar characteristics. In this study, a novel method was developed for the charge variants characterization of co-formulated antibodies using three-dimensional liquid chromatography–mass spectrometry (3D-LC-MS). Hydrophobic interaction chromatography (HIC) was used as the first dimension to separate and collect the two co-formulated antibodies. The two collections were then injected into the second-dimension IEC separately for charge variants separation and analysis. Subsequently, the separated charge variants underwent on-line desalting in the third-dimension reverse-phase chromatography (RPC) and subsequent mass spectroscopy analysis. The novel method could simultaneously provide a charge variants ratio and post-translational modification (PTM) data for the two co-formulated antibodies. Therefore, it could be used for release testing and stability studies of co-formulated antibodies, making up for the shortcomings of the existing approaches. It was the first time that charge variants of co-formulated antibodies were characterized by the 3D-LC-MS method, to the best of our knowledge. Full article

In this paper, we present a new objective quality metric designed for immersive video applications—IV-SSIM. The proposed IV-SSIM metric is an evolution of our previous work—IV-PSNR (immersive video peak signal-to-noise ratio)—which became a commonly used metric in research and ISO/IEC MPEG standardization activities on immersive video. IV-SSIM combines the advantages of IV-PSNR and metrics based on the structural similarity of images, being able to properly mimic the subjective quality perception of immersive video with its characteristic distortions induced by the reprojection of pixels between multiple views. The effectiveness of IV-SSIM was compared with 16 state-of-the-art quality metrics (including other metrics designed for immersive video). Tested metrics were evaluated in an immersive video coding scenario and against a commonly used image quality database—TID2013—showing their performance in both immersive and typical, non-immersive use cases. As presented, the proposed IV-SSIM metric clearly outperforms other metrics in immersive video applications, while also being highly competitive for 2D image quality assessment. The authors of this paper have provided a publicly accessible, efficient implementation of the proposed IV-SSIM metric, which is used by ISO/IEC MPEG video coding experts in the development of the forthcoming second edition of the MPEG immersive video (MIV) coding standard. Full article

Computer Algebra Systems (CAS) currently lack an effective auditory representation, with most existing solutions relying on screen readers that provide limited functionality. This limitation prevents blind users from fully understanding and interpreting mathematical expressions, leading to confusion and self-doubt. This paper addresses the challenges blind individuals face when comprehending mathematical expressions within a CAS environment. We propose “Math for Everybody” (Math4e, version 1.0), a software module to reduce barriers for blind users in education. Math4e is a Sonification Module for CAS that generates a series of auditory tones, prosodic cues, and variations in audio parameters such as volume and speed. These resources are designed to eliminate ambiguity and facilitate the interpretation and understanding of mathematical expressions for blind users. To assess the effectiveness of Math4e, we conducted standardized tests employing the methodologies outlined in the Software Engineering Body of Knowledge (SWEBOK), International Software Testing Qualifications Board (ISTBQ), and ISO/IEC/IEEE 29119. The evaluation encompassed two scenarios: one involving simulated blind users and another with real blind users associated with the “Asociación de Invidentes Milton Vedado” foundation in Ecuador. Through the SAM methodology and verbal surveys (given the condition of the evaluated user), results are obtained, such as 90.56% for pleasure, 90.78% for arousal, and 91.56% for dominance, which demonstrates significant acceptance of the systems by the users. The outcomes underscored the users’ commendable ability to identify mathematical expressions accurately. Full article

Two types of grounding systems are recommended for use in the international standard IEC 62305-3, Part 3: Physical damage to structures and life hazard. One of these is a radial-based grounding system (type-A), which is used in soil resistivities of up to 3000 $\mathsf{\Omega }$m and is considered in this paper. It is a well-known fact that during lightning strikes, only a part of the grounding wire contributes to dissipating the lightning current into the surrounding soil. This effective part of the grounding system depends on several features, such as soil resistivity, burial depth, and rise time of the dissipated lightning current. The effect of all of these features on the effective length of the type-A grounding system is explored in this paper. A suitable supervised machine learning regression model is developed, which will enable readers to accurately approximate the effective length of the type-A grounding system for realistic values of input features. The trained model in the paper yielded an $R^2$ value of 0.99998 on the test set. In addition, two simple mathematical formulas are also provided, which produce similar but less accurate results ($R^2$ values of 0.989883 and 0.998557, respectively). Full article

SecuriDN v. $0.1$ is a tool for the representation of the assets composing the IT and the OT subsystems of Distributed Energy Resources (DERs) control networks and the possible cyberattacks that can threaten them. It is part of a platform that allows the evaluation of the security risks of DER control systems. SecuriDN is a multi-formalism tool, meaning that it manages several types of models: architecture graph, attack graphs and Dynamic Bayesian Networks (DBNs). In particular, each asset in the architecture is characterized by an attack graph showing the combinations of attack techniques that may affect the asset. By merging the attack graphs according to the asset associations in the architecture, a DBN is generated. Then, the evidence-based and time-driven probabilistic analysis of the DBN permits the quantification of the system security level. Indeed, the DBN probabilistic graphical model can be analyzed through inference algorithms, suitable for forward and backward assessment of the system’s belief state. In this paper, the features and the main goals of SecuriDN are described and illustrated through a simplified but realistic case study. Full article

Numerous reviews are available in the literature on PV inverter topologies. These reviews have intensively investigated the available PV inverter topologies from their modulation techniques, control strategies, cost, and performance aspects. However, their compliance with industrial standards has not been investigated in detail so far in the literature. There are various standards such as North American standards (UL1741, IEEE1547, and CSA 22.2) and Australian and European safety standards and grid codes, which include IEC 62109 and VDE. These standards provide detailed guidelines and expectations to be fulfilled by a PV inverter topology. Adherence to these standards is essential and crucial for the successful operation of PV inverters, be it a standalone or grid-tied mode of operation. This paper investigates different PV inverter topologies from the aspect of their adherence to different standards. Both standalone and grid-tied mode of operation-linked conditions have been checked for different topologies. This investigation will help power engineers in selecting suitable PV inverter topology for their specific applications. Full article

Staphylococcus aureus, being one of the most common human pathogens, is responsible for infections in both hospital and community settings. Its virulence is attributed to its ability to evade the immune system by producing immune evasion (IE) proteins. The aim of this study was to detect the frequency of selected IE genes (spin, sbi, sea, sak, chp, scin, sep, ecb), belonging to the immune evasion cluster (IEC), and IEC types in 86 methicillin-susceptible S. aureus (MSSA) strains isolated from unrelated outpatients. In order to determine the diversity of analyzed strains, the phylogenetic relatedness was also determined. All strains were examined for the presence of IE genes using polymerase chain reaction assay. To analyze the clonal relatedness of S. aureus, pulsed-field gel electrophoresis (PFGE) was performed. All analyzed strains harbored the scn gene, followed by sbi (95.4%), ecb (91.7%), spin (89.5%), sak (83.7%), chp (67.4%), sep (67.4%) and sea (5.8%). Seventy-three (84.9%) S. aureus strains were classified into IEC types, of which, IEC type F was most commonly observed. IEC type A was not detected. PFGE results showed no association between clonal relatedness and the presence of IE genes/IEC types. In conclusion, the abundant and so diverse repertoire of genes determining invasion in analyzed strains may prove the fact that these strains are highly advanced and adapted to evade the host immune response. Full article