Search Results (121,871)

The vitamin D receptor (VDR) is an important candidate gene in musculoskeletal phenotypes. Polymorphisms in the VDR have been previously associated with several pathologies and muscular strength in athletes and elderly people; however, the literature reported contradictory results. The object of this research was to verify the association between the most studied VDR variants (rs2228570, rs7975232, and rs1544410) and the increase in muscle mass in elite young soccer players. A sample of 55 soccer players (15–18 years old) from a professional team were selected for this study. DNA was extracted by the salting-out method, and polymorphisms were genotyped by PCR-RFLP, followed by 2% agarose gel electrophoresis. To test the effect of the three SNPs (single nucleotide polymorphisms), a logistic regression analysis was applied. The body composition determination was carried out through the skinfold thickness method, and the muscular area of the arm and lower limb were calculated using the Frisancho formula. All three polymorphisms met the Hardy–Weinberg equilibrium (p > 0.05) and their frequencies fell within the worldwide variability. A significant correlation between rs1544410 and the increase in calf muscle mass was observed. Individuals carrying the A allele showed higher calf muscular mass than those carrying the G allele (p = 0.034). Moreover, a haplotype analysis applied to the two SNPs in linkage disequilibrium (rs7975232 and rs1544410) showed that the AG haplotype appeared negatively correlated to the calf muscle area. In conclusion, we confirm an association between VDR polymorphisms and muscular mass that could encourage the genetic screening of the VDR gene to identify a potential risk of injury and for individual nutritional interventions. Full article

Microgel particles can play a key role, e.g., in drug delivery systems, tissue engineering, advanced (bio)sensors or (bio)catalysis. Amine-functionalized microgels are particularly interesting in many applications since they can provide pH responsiveness, chemical functionalities for, e.g., bioconjugation, unique binding characteristics for pollutants and interactions with cell surfaces. Since the incorporation of amine functionalities in controlled amounts with predefined architectures is still a challenge, here, we present a novel method for the synthesis of responsive core–shell nanogels (d_h < 100 nm) with a poly(N-isopropylacrylamide) (pNIPAm) core and a polyamine shell. To achieve this goal, a surface-functionalized pNIPAm nanogel was first prepared in a semi-batch precipitation polymerization reaction. Surface functionalization was achieved by adding acrylic acid to the reaction mixture in the final stage of the precipitation polymerization. Under these conditions, the carboxyl functionalities were confined to the outer shell of the nanogel particles, preserving the core’s temperature-responsive behavior and providing reactive functionalities on the nanogel surface. The polyamine shell was prepared by the chemical coupling of polyethyleneimine to the nanogel’s carboxyl functionalities using a water-soluble carbodiimide (EDC) to facilitate the coupling reaction. The efficiency of the coupling was assessed by varying the EDC concentration and reaction temperature. The molecular weight of PEI was also varied in a wide range (M_w = 0.6 to 750 kDa), and we found that it had a profound effect on how many polyamine repeat units could be immobilized in the nanogel shell. The swelling and the electrophoretic mobility of the prepared core–shell nanogels were also studied as a function of pH and temperature, demonstrating the successful formation of the polyamine shell on the nanogel core and its effect on the nanogel characteristics. This study provides a general framework for the controlled synthesis of core–shell nanogels with tunable surface properties, which can be applied in many potential applications. Full article

The use of fibroblast activation protein inhibitors (FAPis) for positron emission tomography (PET) imaging in cancer has garnered significant interest in recent years, yielding promising results in preclinical and clinical settings. FAP is predominantly expressed in pathological conditions such as fibrosis and cancer, making it a compelling target. An optimized approach involves using FAPi homodimers as PET tracers, which enhance tumor uptake and retention, making them more effective candidates for therapy. Here, a UAMC-1110 inhibitor-based homodimer, DOTAGA-Glu(FAPi)₂, was synthesized and radiolabeled with gallium-68, and its efficacy was evaluated in vivo for PET imaging in an endogenously FAP-expressing xenografted mouse model, U87MG. Notably, 45 min post-injection, the mean uptake of [⁶⁸Ga]Ga-DOTAGA-Glu(FAPi)₂ was 4.7 ± 0.5% ID/g in the tumor with low off-target accumulation. The ex vivo analysis of the FAP expression in the tumors confirmed the in vivo results. These findings highlight and confirm the tracer’s potential for diagnostic imaging of cancer and as a theranostic companion. Full article

Background and Objectives: Neglected patellar dislocation in the presence of end-stage osteoarthritis (OA) is a rare condition characterized by the patella remaining laterally dislocated without reduction. Due to the scarcity of reported cases, the optimal management approach is still uncertain. However, primary total knee arthroplasty (TKA) can serve as an effective treatment option. This study aimed to present the clinical and radiological outcomes achieved using our surgical technique. Materials and Methods: A retrospective review of 12 knees in 8 patients with neglected patellar dislocation and end-stage OA who underwent primary TKA was conducted. The surgical procedure involved conventional TKA techniques (e.g., medial parapatellar arthrotomy) and additional procedures specific to the individual pathologies of neglected patellar dislocation (e.g., lateral release, medial plication, and quadriceps lengthening). Clinical outcomes, including patient-reported outcome measures (PROMs) (Knee Society Scores and the Western Ontario and McMaster Universities Osteoarthritis Index) and knee range of motion (ROM), were assessed preoperatively and two years postoperatively. Radiological measures including mechanical femorotibial angle and patellar tilt angle were assessed preoperatively and until the last follow-up examinations. Any complications were also reviewed. Results: There were significant improvements in all PROMs, knee ROM, and radiological outcomes, including mechanical femorotibial angle and patellar tilt angle (all p < 0.05). At a mean follow-up of 68 months, no major complications requiring revision surgery, including patellar dislocation, were reported. Conclusions: Primary TKA is an effective procedure for correcting various pathologies associated with neglected patellar dislocation in end-stage OA without necessitating additional bony procedures. Satisfactory clinical and radiological outcomes can be expected using pathology-specific procedures. Full article

This study focuses on long concrete interfaces tested under cyclic actions, fastened with post-installed industrial steel screws. The overall behavior and the effect of roughness were investigated in three long interfaces, representative of connections between, e.g., a slab and a wall, a beam and a wall, etc. The results were compared with those of short interfaces tested by the authors in previous campaigns. It was observed that rough long interfaces activate their maximum resistance at small values of imposed shear slip. When roughness was reduced, the maximum resistance was also reduced, the corresponding shear slip was increased, and the overall behavior was stable. For large values of the shear slip, imposed at one end of the interface, the shear slips along it tended to be uniform, both in short and long interfaces. The limited embedment length of the screws led to their pronounced pullout. Finally, the asymmetry of resistance between the two loading directions that was observed in short interfaces was alleviated in the long ones, where also the scatter of the results was limited among duplicate specimens. Full article

Terahertz (THZ) spectroscopy has emerged as a superior label-free sensing technology in the detection, identification, and quantification of biomolecules in various biological samples. However, the limitations in identification and discrimination sensitivity of current methods impede the wider adoption of this technology. In this article, a meticulously designed metasurface is proposed for molecular fingerprint enhancement, consisting of a periodic array of lithium tantalate triangular prism tetramers arranged in a square quartz lattice. The physical mechanism is explained by the finite-difference time-domain (FDTD) method. The metasurface achieves a high quality factor (Q-factor) of 231 and demonstrates excellent THz sensing capabilities with a figure of merit (FoM) of 609. By varying the incident angle of the THz wave, the molecular fingerprint signal is strengthened, enabling the highly sensitive detection of trace amounts of analyte. Consequently, cinnamoylglycine can be detected with a sensitivity limit as low as $1.23 \mathsf{\mu }\mathrm{g}·{\mathrm{c}\mathrm{m}}^{-2}$. This study offers critical insights into the advanced application of THz waves in biomedicine, particularly for the detection of urinary biomarkers in various diseases, including gestational diabetes mellitus (GDM). Full article

Probiotics in aquaculture hold promise for enhancing fish health and growth. Due to their increased specificity and affinity for their host, indigenous probiotics may offer isolated and potentially amplified benefits. This study investigated the effects of Lactococcus lactis PH3-05, previously isolated from adults of tropical gar (Atractosteus tropicus), on the growth, survival, digestive enzyme activity, intestinal morphology, expression of barrier and immune genes, and intestinal microbiota composition in the larvae of tropical gar. Larvae were fed with live L. lactis PH3-05 concentrations of 10⁴, 10⁶, and 10⁸ CFU/g for 15 days alongside a control diet without probiotics. Higher concentrations of L. lactis PH3-05 (10⁶ and 10⁸ CFU/g) positively influenced larval growth, increasing hepatocyte area and enterocyte height. The 10⁶ CFU/g dose significantly enhanced survival (46%) and digestive enzyme activity. Notably, the 10⁸ CFU/g dose stimulated increased expression of muc-2 and il-10 genes, suggesting enhanced mucosal barrier function and anti-inflammatory response. Although L. lactis PH3-05 did not significantly change the diversity, structure, or Phylum level composition of intestinal microbiota, which was constituted by Proteobacteria, Bacteroidota, Chloroflexi, and Firmicutes, an increase in Lactobacillus abundance was observed in fish fed with 10⁶ CFU/g, suggesting enhanced probiotic colonization. These results demonstrate that administering L. lactis PH3-05 at 10⁶ CFU/g promotes growth, survival, and digestive health in A. tropicus larvae, establishing it as a promising indigenous probiotic candidate for aquaculture applications. Full article

The present study examines the role of maternal years of education and family language policy (FLP) in monolingual and bilingual children’s acquisition of Hebrew plural morphology. The case of the Hebrew plural system is especially interesting when examining the influence of the above factors on morphological performance, given that it demands both a mastery of morphological rules (characterized by a high degree of transparency in Hebrew) and a lexicon-based mastery of exceptions. Participants were 146 children, 74 bilinguals (heritage language: Russian; societal language: Hebrew) and 72 Hebrew monolinguals, aged 5–8 (kindergarten, first grade, and second grade), from the same schools and neighborhoods. A Hebrew pluralization, sentence completion task that included 99 items from two categories: fully regular words whose plural forms are based on a morphological rule and non-regular words whose plural forms (also) require lexical and/or morpho-lexical knowledge. The parents of the bilingual children filled out a questionnaire with questions on background variables (e.g., maternal education) and language practice in both languages by different family members and language use at home. The findings indicated that maternal education contributes differently and distinctly to the linguistic performance of children from different linguistic backgrounds. For monolingual children, an increase in the number of years of maternal education is associated with an increase in the likelihood of success in the lexical and morpho-lexical aspects of Hebrew. By contrast, for bilingual children, no significant contribution of maternal education to children’s performance was found. For bilingual participants, their performance in the lexical and morpho-lexical aspects of the Hebrew plural system was consistently influenced by FLP across all school settings—increased use of Russian at home was associated with a lower likelihood of success in the societal language. FLP characteristics were not found to be related to maternal education. These findings have clinical implications for both assessment and intervention processes when working with bilingual children. Full article

Employing molecular dynamics software, three models—vacuum–cellulose, nitrogen–cellulose, and air–cellulose—were built to clarify, via a microscopic perspective, the macroscopic changes in single-chain cellulose undergoing vacuum, nitrogen, and air heat treatments. Kinetic simulations were run following model equilibrium within the NPT system of 423, 443, 463, 483, and 503 K. The energy variations, cell parameters, densities, mean square displacements, hydrogen bonding numbers, and mechanical parameters were analyzed for the three models. The findings demonstrate that as the temperature climbed, the cellular characteristics among two models—the nitrogen and vacuum models—decreased and subsequently increased. The nitrogen model reached its lowest value at 443 K. In contrast, the vacuum model reached its minimum value at 463 K. The vacuum heat treatment may enhance the structural stability of the single-chain cellulose more effectively than the nitrogen and air treatments because it increases the number of hydrogen bonds within the cellulose chain and stabilizes the mean square displacement. Furthermore, the temperature has an impact on the mechanical characteristics of the cellulose amorphous zone; the maximum values of E and G for the vacuum and nitrogen models are found at 463 and 443 K, respectively. The Young’s modulus and shear modulus were consistently more significant for the vacuum model at either temperature, and the Poisson’s ratio was the opposite. Therefore, the vacuum heat treatment can better maintain wood stiffness and deformation resistance, thus improving wood utilization. These findings provide an essential theoretical basis for wood processing and modification, which can help optimize the heat treatment and enhance wood’s utilization and added value. Full article

This study explores the potential of biochar derived from microwave-assisted catalytic pyrolysis of solid digestate as an additive to enhance the stability and performance of the anaerobic digestion process. The focus was placed on the effects of biochar dosage, pyrolysis temperature, and pyrolysis catalyst on methane production. Biochemical methane potential (BMP) tests using synthetic food waste as the substrate revealed a dosage-dependent relationship with specific methane yield (SMY). At a low biochar dosage of 0.1 g/g total solids (TS), improvement in methane (CH₄) production was marginal, whereas a high dosage of 0.6 g/g TS increased CH₄ content by at least 10% and improved yield by 35–52%. ANOVA analysis indicated that biochar dosage level significantly influenced CH₄ yield, while pyrolysis temperature (400 °C vs. 500 °C) and catalyst (20 wt% K₃PO₄ vs. 10 wt% K₃PO₄/10 wt% clinoptilolite) did not lead to significant differences in CH₄ yield between the treatments. Correlation analysis results suggested that biochar’s most impactful properties on methane yield would be dosage-adjusted specific surface area (or total surface area per unit volume of substrate) and aromaticity index. The findings underscore the potential of solid-digestate-derived biochar as a beneficial additive for anaerobic digestion and hence the sustainability of food waste management system. Full article

Background and objectives: Hereditary spastic paraplegia (HSP) is characterized by unsteady gait, motor incoordination, speech impairment, abnormal eye movement, progressive spasticity and lower limb weakness. Spastic paraplegia 75 (SPG75) results from a mutation in the gene that encodes myelin associated glycoprotein (MAG). Only a limited number of MAG variants associated with SPG75 in families of European, Middle Eastern, North African, Turkish and Palestinian ancestry have been documented so far. This study aims to provide further insight into the clinical and molecular manifestations of HSP. Methods: Using whole-exome sequencing, we investigated a consanguineous Pakistani family where three individuals presented with clinical signs of HSP. Sanger sequencing was used to carry out segregation analysis on available family members, and a minigene splicing assay was utilized to evaluate the effect of the splicing variant. Results: We identified a novel homozygous pathogenic splice donor variant in MAG (c.46 + 1G > T) associated with SPG75. RNA analysis revealed exon skipping that resulted in the loss of a start codon for ENST00000361922.8 isoform. Affected individuals exhibited variable combinations of nystagmus, developmental delay, cognitive impairments, spasticity, dysarthria, delayed gait and ataxia. The proband displayed a quadrupedal stride, and his siblings experienced frequent falls and ataxic gait as one of the prominent features that have not been previously reported in SPG75. Conclusions: Thus, the present study presents an uncommon manifestation of SPG75, the first from the Pakistani population, and broadens the spectrum of MAG variants. Full article

Background and Objectives: To evaluate the clinical findings of glucose 6-phosphate dehydrogenase (G6PD) and pyruvate kinase (PK) deficiency in prolonged jaundice and to determine whether the systemic immune inflammation index (SII), neutrophil-to-lymphocyte ratio (NLR), and platelet-to-lymphocyte ratio (PLR) can be used in the diagnosis of neonatal prolonged jaundice. Materials and Methods: Among full-term neonates with hyperbilirubinemia who were admitted to Medicine Hospital between January 2019 and January 2024 with the complaint of jaundice, 167 infants with a serum bilirubin level above 10 mg/dL, whose jaundice persisted after the 10th day, were included in this study. Results: G6PD activity was negatively correlated with NLR, SII, age, and hematocrit (Hct). There was a weak negative correlation between G6PD and NLR and a moderate negative correlation between G6PD activity and SII when adjusted for age and Hct. PK activity showed no significant correlation with G6PD, NLR, PLR, SII, age, and Hct. A linear relationship was observed between G6PD activity and SII and NLR. Conclusions: NLR and SII can be easily calculated in the evaluation of prolonged jaundice in G6PD deficiency has a considerable advantage. NLR and SII levels may contribute by preventing further tests for prolonged jaundice and regulating its treatment. It may be useful to form an opinion in emergencies and in early diagnostic period. Full article

Ultra-High-Performance Concrete (UHPC) and Ultra-High-Performance Fiber-Reinforced Concrete (UHPFRC) represent promising materials in the field of construction, offering exceptional strength and durability, making them ideal for the development of a wide range of infrastructure projects. One of the goals is to better understand the impact of each component of the materials on their key properties in the hardened state. This work examines the effect of the aggregate on the properties of UHPC and UHPFRC. This article provides test results for five compositions without fiber, and five compositions with 2% corrugated steel fiber. Three aggregate concentrations (0, 0.2, and 0.4 ${\mathrm{m}}^3$) and quartz sand with different maximum particle sizes (0.4 and 0.8 mm) were selected. It was found that the mechanical properties of the material, such as the steel fiber bond strength, compressive and axial tensile strength, fracture energy, and critical stress intensity factor, depend on both the concentration of the aggregate and the size of its particles. A novel mix-design parameter was proposed, which reflects the total surface area of the aggregate in the composition ($S_{agg,tot}$). The relationships between the parameter $S_{agg,tot}$ and the mechanical characteristics of UHPC and UHPFRC were established. The steel fiber bond strength, axial tensile strength, and fracture energy-related parameters grew non-linearly when the parameter $S_{agg,tot}$ increased. When the parameter $S_{agg,tot}$ was changed from 0 to 12.38 · ${10}^3{ \mathrm{m}}^2$, the fiber bond strength increased by 1.38 times. The axial tensile strength and total fracture energy of the UHPFRC increased by 1.48 and 1.63 times, respectively. The compressive strength changed linearly and increased by 1.12 times. The improvement in the mechanical properties of the material was associated with an increase in the friction force between the fiber and the matrix, which was confirmed by the formation of a greater number of scratches on the surface of the fiber with an increasing value of the parameter $S_{agg,tot}$. The deformation characteristics, such as modulus of elasticity, Poisson’s ratio, and drying shrinkage strain, were determined solely by the volumetric concentration of the aggregate, as in conventional concrete. An increase in the aggregate volume content from 0 to 0.4 ${\mathrm{m}}^3$ led to an increase in the modulus of elasticity of 1.41–1.44 times, and a decrease in the ultimate shrinkage strain of almost 2 times. The dependencies obtained in this work can be used to predict the properties of UHPC and UHPFRC, taking into account the type and volume concentration of the aggregate. Full article

Potatoes (Solanum tuberosum L.) are an important plant crop, whose yield may vary significantly depending on pedo-climatic conditions and genotype. Therefore, the analysis of the genotype × environment interaction (GEI) is mandatory for the setup of high-yielding and stable potato genotypes. This research evaluated the tuber yield (t ha⁻¹) and yield characteristic of nine potato cultivars over 3 years and 4 organic farms in Poland by additive main effects and multiplicative interactions (AMMIs) and genotype plus genotype environment interaction (GGE) biplot analyses. The results of these analyses indicated significant differentiation of tuber yield among genotypes in individual environments. It was found that the environment (E, where E = L (localization) × Y (year)), genotype (G) and GEI, but not replication, significantly affected tuber yield. The AMMI analysis showed that the environment factor explained the most considerable part of tuber yield variations (52.3%), while the GEI and G factors explained a much lower part of the variations. The AMMI and GGE analyses identified five cvs.: Twister (46.4 t ha⁻¹), Alouette (35.8 t ha⁻¹), Kokra (34.8 t ha⁻¹), Levante (33.1 t ha⁻¹), and Gardena (30.4 t ha⁻¹), as leading cultivars in the studied organic farms due to their high productivity coupled with yield stability. The statistical measure Kang (YS_i) showed that these cvs. can be considered as adaptable to a wide range of organic environments. In the case of morphological traits of tubers (tuber shape and depth of tuber eyes), the most important factor influencing both these traits was genotype (G). Influence of other factors, like localization (L), year (Y), and all interactions (double and triple), were much less significant or insignificant. In case of taste and non-darkening of tuber flesh, the main effects which significantly affected the values of these traits were genotype (G) and localization (L). We observed that cooking type can vary depending on the year (Y) and the localization (L). Full article

This study explores the effect of financial statement comparability on environmental, social, and governance (ESG) performance. Comparability is a qualitative characteristic that enhances the usefulness of accounting information, as emphasized in the conceptual framework of accounting standards. Reporting accounting information with high comparability reduces the cost of obtaining information for users of financial statements, thereby enabling them to make decisions more efficiently. However, increased comparability of financial statements may lead managers to avoid investments that do not directly and rapidly enhance corporate value. In the context of ESG, this behavior could lead to a reduction in ESG investments, thereby lowering overall ESG performance. This study empirically investigates this hypothesis by analyzing data from listed firms in Korea. The analysis reveals a negative relationship between the comparability of financial statements and ESG performance. When breaking down ESG performance into individual components, the environmental (E) and social (S) factors show a negative relationship with comparability, while no significant relationship is observed with the governance (G) factor. The results remain consistent across various additional tests, including the use of alternative comparability and ESG proxies, as well as when employing firm fixed effects models. The findings of this study highlight the potential adverse effects of financial statement comparability on ESG performance. These results suggest that while comparability facilitates better decision-making through enhanced information efficiency, it may inadvertently discourage managers from engaging in ESG investments, thereby negatively impacting a company’s sustainability. Full article