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Agriculture, Volume 14, Issue 1 (January 2024) – 162 articles

Cover Story (view full-size image): Climate plays a role in N management through changes in crop calendars, soil properties, agronomic practices, and yield effects. Multiple data sources indicate (but not prove) that colder climates are generally associated with higher levels of SOM-associated N stocks, but this may not result in greater crop N availability. Also, colder climates are on average associated with lower EONRs, which are mostly explained by lower yield expectations. But seasonal weather is an important superimposing factor impacting the EONR in all climates. Progress in N management therefore requires a dynamic approach that accounts for the effects of climate and weather with interacting agronomic factors for a specific growing environment. View this paper
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12 pages, 1646 KiB  
Article
Evaluation of Biochar Addition to Digestate, Slurry, and Manure for Mitigating Carbon Emissions
by Leonardo Verdi, Anna Dalla Marta, Simone Orlandini, Anita Maienza, Silvia Baronti and Francesco Primo Vaccari
Agriculture 2024, 14(1), 162; https://doi.org/10.3390/agriculture14010162 - 22 Jan 2024
Cited by 1 | Viewed by 1854
Abstract
The contribution of animal waste storage on GHG emissions and climate change is a serious issue for agriculture. The carbon emissions that are generated from barns represent a relevant source of emissions that negatively affect the environmental performance measures of livestock production. In [...] Read more.
The contribution of animal waste storage on GHG emissions and climate change is a serious issue for agriculture. The carbon emissions that are generated from barns represent a relevant source of emissions that negatively affect the environmental performance measures of livestock production. In this experiment, CO2 and CH4 emissions from different animal wastes, namely, digestate, slurry, and manure, were evaluated both in their original form and with a biochar addition. The emissions were monitored using the static camber methodology and a portable gas analyzer for a 21-day period. The addition of biochar (at a ratio of 2:1 between the substrates and biochar) significantly reduced the emissions of both gases compared to the untreated substrates. Slurry exhibited higher emissions due to its elevated gas emission tendency. The biochar addition reduced CO2 and CH4 emissions by 26% and 21%, respectively, from the slurry. The main effect of the biochar addition was on the digestate, where the emissions decreased by 45% for CO2 and 78% for CH4. Despite a lower tendency to emit carbon-based gases of manure, biochar addition still caused relevant decreases in CO2 (40%) and CH4 (81%) emissions. Biochar reduced the environmental impacts of all treatments, with a GWP reduction of 55% for the digestate, 22% for the slurry, and 44% for the manure. Full article
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<p>Example of the static chamber used in the experiment, with details about the fan used for homogenizing the gas sample.</p>
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<p>Maximum, minimum, and average air temperature trends during the experimental period.</p>
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<p>Trend of daily CO<sub>2</sub>-C emissions from D and DB (<b>a</b>), S and SB (<b>b</b>), and M and MB (<b>c</b>). The error bars represent the daily standard deviations for each treatment.</p>
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<p>Trend of daily CH<sub>4</sub>-C emissions from D and DB (<b>a</b>), S and SB (<b>b</b>), and M and MB (<b>c</b>). The error bars represent the daily standard deviations for each treatment.</p>
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<p>Global warming potential for the digestate (D), digestate plus biochar (DB), slurry (S), slurry plus biochar (SB), manure (M), and manure plus biochar (MB). The error bars represent the standard deviations for each treatment. The bars that do not share a letter were significantly different at a 5% probability level.</p>
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30 pages, 3540 KiB  
Review
Applying Remote Sensing, Sensors, and Computational Techniques to Sustainable Agriculture: From Grain Production to Post-Harvest
by Dágila Melo Rodrigues, Paulo Carteri Coradi, Newiton da Silva Timm, Michele Fornari, Paulo Grellmann, Telmo Jorge Carneiro Amado, Paulo Eduardo Teodoro, Larissa Pereira Ribeiro Teodoro, Fábio Henrique Rojo Baio and José Luís Trevizan Chiomento
Agriculture 2024, 14(1), 161; https://doi.org/10.3390/agriculture14010161 - 22 Jan 2024
Cited by 2 | Viewed by 2616
Abstract
In recent years, agricultural remote sensing technology has made great progress. The availability of sensors capable of detecting electromagnetic energy and/or heat emitted by targets improves the pre-harvest process and therefore becomes an indispensable tool in the post-harvest phase. Therefore, we outline how [...] Read more.
In recent years, agricultural remote sensing technology has made great progress. The availability of sensors capable of detecting electromagnetic energy and/or heat emitted by targets improves the pre-harvest process and therefore becomes an indispensable tool in the post-harvest phase. Therefore, we outline how remote sensing tools can support a range of agricultural processes from field to storage through crop yield estimation, grain quality monitoring, storage unit identification and characterization, and production process planning. The use of sensors in the field and post-harvest processes allows for accurate real-time monitoring of operations and grain quality, enabling decision-making supported by computer tools such as the Internet of Things (IoT) and artificial intelligence algorithms. This way, grain producers can get ahead, track and reduce losses, and maintain grain quality from field to consumer. Full article
(This article belongs to the Special Issue Agricultural Products Processing and Quality Detection)
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<p>Index of publication per year (<b>A</b>) and area (<b>B</b>).</p>
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<p>Remote sensing techniques, their characteristics, applications, and sensors.</p>
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<p>Application of vegetation sensors in agriculture.</p>
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<p>Illustration of a probe for monitoring temperature, relative humidity, carbon dioxide levels, and logistical information during the grain transportation.</p>
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<p>Illustration of a grain monitoring system during drying.</p>
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<p>Illustration of a grain monitoring system during storage.</p>
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<p>Summary of the monitoring the grain production chain: production to post-harvest.</p>
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<p>Illustration of the whole grain production system managed by the producer through the latest technologies of remote sensing, monitoring, internet of things, and artificial intelligence.</p>
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15 pages, 2032 KiB  
Article
Comparative Analysis of Climate Change Impacts on Climatic Variables and Reference Evapotranspiration in Tunisian Semi-Arid Region
by Basma Latrech, Taoufik Hermassi, Samir Yacoubi, Adel Slatni, Fathia Jarray, Laurent Pouget and Mohamed Ali Ben Abdallah
Agriculture 2024, 14(1), 160; https://doi.org/10.3390/agriculture14010160 - 22 Jan 2024
Cited by 2 | Viewed by 1327
Abstract
Systematic biases in general circulation models (GCM) and regional climate models (RCM) impede their direct use in climate change impact research. Hence, the bias correction of GCM-RCMs outputs is a primary step in such studies. This study compares the potential of two bias [...] Read more.
Systematic biases in general circulation models (GCM) and regional climate models (RCM) impede their direct use in climate change impact research. Hence, the bias correction of GCM-RCMs outputs is a primary step in such studies. This study compares the potential of two bias correction methods (the method from the third phase of the Inter-Sectoral Impact Model Intercomparison Project (ISIMIP3) and Detrended Quantile Matching (DQM)) applied to the raw outputs of daily data of minimum and maximum air temperatures and precipitation, in the Cap-Bon region, from eight GCM-RCM combinations. The outputs of GCM/RCM combinations were acquired from the European branch of the coordinated regional climate downscaling experiment (EURO-CORDEX) dataset for historical periods and under two representative concentration pathway (RCP4.5 and RCP8.5) scenarios. Furthermore, the best combination of bias correction/GCM-RCM was used to assess the impact of climate change on reference evapotranspiration (ET0). Numerous statistical indicators were considered to evaluate the performance of the bias correction/historical GCM-RCMs compared to the observed data. Trends of the Hargreaves–Samani_ET0 model during the historical and projected periods were determined using the TFPMK method. A comparison of the bias correction methods revealed that, for all the studied model combinations, ISIMIP3 performs better in reducing biases in monthly precipitation. However, for Tmax and Tmin, the biases are greatly removed when the DQM bias correction method is applied. In general, better results were obtained when the HadCCLM model was used. Before applying bias correction, the set of used GCM-RCMs projected reductions in precipitation for most of the months compared to the reference period (1982–2006). However, Tmin and Tmax are expected to increase in all months and for the three studied periods. Hargreaves–Samani ET0 values obtained from the best combination (DQM/ HadCCLM) show that RCP8.5 (2075–2098) will exhibit the highest annual ET0 increase compared to the RCP4.5 scenario and the other periods, with a change rate equal to 11.85% compared to the historical period. Regarding spring and summer seasons, the change rates of ET0 are expected to reach 10.44 and 18.07%, respectively, under RCP8.5 (2075–2098). This study shows that the model can be used to determine long-term trends in ET0 patterns for diverse purposes, such as water resources planning, agricultural crop management and irrigation scheduling in the Cap-Bon region. Full article
(This article belongs to the Section Ecosystem, Environment and Climate Change in Agriculture)
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<p>(<b>a</b>) Taylor diagram for comparison between the performances of bias correction methods and GCM-RCMs in reproducing monthly precipitation; and (<b>b</b>) percent bias in monthly precipitation with the two bias correction methods for all GCM-RCMs combinations.</p>
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<p>Taylor diagram for comparison between performances of GCM-RCM model outputs, after bias correction using two different methods, in reproducing monthly maximum (<b>a</b>); and minimum (<b>b</b>) air temperatures in the Cap-Bon region.</p>
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<p>Boxplots representing the projected changes in mean monthly precipitation, minimum and maximum air temperature, and median values of the raw model outputs under the RCP4.5 scenario. The black horizontal line and the black cross show the models’ median and means, respectively.</p>
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<p>Patterns of annual ET<sub>0</sub> for historical and RCP scenarios.</p>
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16 pages, 1857 KiB  
Article
Consumers’ WTP for Sustainability Turfgrass Attributes with Consideration of Aesthetic Attributes and Water Conservation Policies
by Hyojae Jung and Chanjin Chung
Agriculture 2024, 14(1), 159; https://doi.org/10.3390/agriculture14010159 - 22 Jan 2024
Cited by 1 | Viewed by 1065
Abstract
This study estimates consumers’ willingness to pay (WTP) for sustainability turfgrass attributes such as low-input and stress-tolerance attributes, while considering potential trade-off relationships between aesthetic attributes and sustainability attributes. To address our objectives, our study conducts a choice experiment and estimates two mixed [...] Read more.
This study estimates consumers’ willingness to pay (WTP) for sustainability turfgrass attributes such as low-input and stress-tolerance attributes, while considering potential trade-off relationships between aesthetic attributes and sustainability attributes. To address our objectives, our study conducts a choice experiment and estimates two mixed logit models. The first model includes low-input, winter kill, and shade-tolerance attributes as predictor variables, and the second model extends the first model by adding interaction terms between the aesthetic and sustainability attributes. Another choice experiment is conducted under water policies with various water rate increase and watering restriction scenarios. Results from the mixed logit models show that, overall, higher low-input cost reduction, less winter-damaged, and more shade-tolerant grasses are preferred, and that the direct effect of aesthetic attributes on consumers’ preferences is strong, but the indirect effects represented by the interaction terms are generally statistically insignificant. Our results indicate that consumers like to have a pretty lawn, but no strong consideration is given to the aesthetics of their lawn when selecting low-input and stress-tolerant turfgrasses. Our choice experiment under water policy scenarios suggests that water pricing is more effective than watering restriction in increasing consumer demand for water-conserving turfgrasses. Full article
(This article belongs to the Section Agricultural Economics, Policies and Rural Management)
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<p>An Example of Choice Set.</p>
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14 pages, 973 KiB  
Article
Effect of Pulsed Electric Field Treatment on Seed Germination and Seedling Growth of Scutellaria baicalensis
by Yanbo Song, Weiyu Zhao, Zhenxian Su, Shuhong Guo, Yihan Du, Xinyue Song, Xiaojing Shi, Xiaofeng Li, Yuli Liu and Zhenyu Liu
Agriculture 2024, 14(1), 158; https://doi.org/10.3390/agriculture14010158 - 22 Jan 2024
Cited by 7 | Viewed by 1864
Abstract
To explore the effects of pulsed electric field treatment on the germination of Scutellaria baicalensis seeds and the growth of seedlings, this study used the response surface methodology to design the working parameters of the pulsed electric field and treated and cultured Scutellaria [...] Read more.
To explore the effects of pulsed electric field treatment on the germination of Scutellaria baicalensis seeds and the growth of seedlings, this study used the response surface methodology to design the working parameters of the pulsed electric field and treated and cultured Scutellaria baicalensis seeds. The results showed that the pulsed electric field treatment was beneficial for the germination of Scutellaria baicalensis seeds, improving the metabolic activity and stress resistance of seedlings. When the pulsed electric field treatment’s parameters were 0.5 kV·cm−1, 120 μs, and 99 pulses, the germination potential of seeds was significantly increased by 29.25% and the germination index significantly increased by 20.65%, compared to the control. From 5th to 15th day, the activities of SOD, POD, and α-amylase in the seedlings, and the contents of Pro, soluble sugars, and soluble proteins were significantly increased, compared to the control. This study provides a theoretical basis for improving the germination and seedling growth of medicinal herbs such as Scutellaria baicalensis and their practical application in production. Full article
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<p>Effects of different PEF treatments on metabolism of seedlings: (<b>a</b>) the activities of SOD in seedlings; (<b>b</b>) the activities of POD in seedlings; (<b>c</b>) the content of soluble sugars in seedlings; (<b>d</b>) the content of soluble proteins in seedlings; (<b>e</b>) the activities of α-amylase in seedlings; and (<b>f</b>) the content of proline in seedlings. The ‘*’ means <span class="html-italic">p</span> &lt; 0.05; ** means <span class="html-italic">p</span> &lt; 0.01; *** means <span class="html-italic">p</span> &lt; 0.0005.</p>
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16 pages, 3373 KiB  
Review
Characteristics and Migration Dynamics of Microplastics in Agricultural Soils
by Yuxin Deng, Zijie Zeng, Weiying Feng, Jing Liu and Fang Yang
Agriculture 2024, 14(1), 157; https://doi.org/10.3390/agriculture14010157 - 21 Jan 2024
Cited by 1 | Viewed by 2787
Abstract
The risks brought by microplastics (MPs) to agricultural soil structure and crop growth in the agricultural system are the focus of global debate. MPs enter the soil through various routes, such as through the use of agricultural mulch and atmospheric deposition. Here, we [...] Read more.
The risks brought by microplastics (MPs) to agricultural soil structure and crop growth in the agricultural system are the focus of global debate. MPs enter the soil through various routes, such as through the use of agricultural mulch and atmospheric deposition. Here, we review the research on MP pollution in the soil during the last 30 years. This review focuses on (i) the sources, types, and distribution characteristics of MPs in agricultural soils; (ii) the migration and transformation of MPs and their interactions with microorganisms, organic matter, and contaminants in agricultural soils; and (iii) the effects of environmental factors on the composition and structure of MPs in agricultural soils. This review also proposes key directions for the future research and management of MPs in the agricultural soil. We aim to provide a theoretical basis for the fine management of agricultural farmland. Full article
(This article belongs to the Special Issue Impact of Plastics on Agriculture)
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<p>The number of MPs and soil MPs articles published from 1995 to 2024 ((<b>A</b>) the number of MPs articles and the number of soil MPs articles; and (<b>B</b>) proportion of soil MPs in MPs studies).</p>
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<p>Keyword co-occurrence analysis of soil microplastics research from 2012 to 2024. The size of nodes and fonts is related to the number of co-occurrences.</p>
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<p>Main sources and migration of MPs in the soil.</p>
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<p>Complex interactions of plant roots and microplastics with other substances in soil. (Soil MPs interact with plant roots, organic matter, and nutrients in the soil).</p>
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<p>Microscopic interactions of microplastics with other substances in the soil.</p>
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<p>Schematic diagram of enzymatic degradation mechanism of PA MPs. (PBM: polymer binding module. PA: polyamidase. h: active site.).</p>
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17 pages, 5417 KiB  
Article
Lightweight Pig Face Feature Learning Evaluation and Application Based on Attention Mechanism and Two-Stage Transfer Learning
by Zhe Yin, Mingkang Peng, Zhaodong Guo, Yue Zhao, Yaoyu Li, Wuping Zhang, Fuzhong Li and Xiaohong Guo
Agriculture 2024, 14(1), 156; https://doi.org/10.3390/agriculture14010156 - 21 Jan 2024
Cited by 3 | Viewed by 1552
Abstract
With the advancement of machine vision technology, pig face recognition has garnered significant attention as a key component in the establishment of precision breeding models. In order to explore non-contact individual pig recognition, this study proposes a lightweight pig face feature learning method [...] Read more.
With the advancement of machine vision technology, pig face recognition has garnered significant attention as a key component in the establishment of precision breeding models. In order to explore non-contact individual pig recognition, this study proposes a lightweight pig face feature learning method based on attention mechanism and two-stage transfer learning. Using a combined approach of online and offline data augmentation, both the self-collected dataset from Shanxi Agricultural University's grazing station and public datasets underwent enhancements in terms of quantity and quality. YOLOv8 was employed for feature extraction and fusion of pig face images. The Coordinate Attention (CA) module was integrated into the YOLOv8 model to enhance the extraction of critical pig face features. Fine-tuning of the feature network was conducted to establish a pig face feature learning model based on two-stage transfer learning. The YOLOv8 model achieved a mean average precision (mAP) of 97.73% for pig face feature learning, surpassing lightweight models such as EfficientDet, SDD, YOLOv5, YOLOv7-tiny, and swin_transformer by 0.32, 1.23, 1.56, 0.43 and 0.14 percentage points, respectively. The YOLOv8-CA model’s mAP reached 98.03%, a 0.3 percentage point improvement from before its addition. Furthermore, the mAP of the two-stage transfer learning-based pig face feature learning model was 95.73%, exceeding the backbone network and pre-trained weight models by 10.92 and 3.13 percentage points, respectively. The lightweight pig face feature learning method, based on attention mechanism and two-stage transfer learning, effectively captures unique pig features. This approach serves as a valuable reference for achieving non-contact individual pig recognition in precision breeding. Full article
(This article belongs to the Section Digital Agriculture)
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<p>The natural environment of the pigsty.</p>
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<p>Pig-face samples in complex environments.</p>
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<p>Effects of data augmentation.</p>
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<p>Before-and-after comparison of the number of data augmentation.</p>
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<p>YOLOv8 network structure.</p>
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<p>Structural comparison diagram of C3 and C2f.</p>
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<p>CA module structure.</p>
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<p>Workflow diagram.</p>
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<p>Curve of <span class="html-italic">mAP</span> and loss on validation set during training of different models (<b>a</b>) is curve of <span class="html-italic">mAP</span>. (<b>b</b>) is curve of loss.</p>
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<p>PR graphs with different attention mechanisms are introduced.</p>
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<p><span class="html-italic">AP</span> values for each pig using different methods.</p>
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<p>Confusion matrix for two-stage model.</p>
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<p>False recognition results of pig faces compared with two-stage models ((<b>a</b>,<b>c</b>,<b>e</b>) is the two-stage model correct recognition result graph, (<b>b</b>,<b>d</b>,<b>f</b>) is other model error recognition result map).</p>
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22 pages, 1973 KiB  
Article
Challenges to Food Security in the Middle East and North Africa in the Context of the Russia–Ukraine Conflict
by Anna Kozielec, Jakub Piecuch, Kamila Daniek and Lidia Luty
Agriculture 2024, 14(1), 155; https://doi.org/10.3390/agriculture14010155 - 21 Jan 2024
Cited by 1 | Viewed by 3288
Abstract
In this article, the impact of the Russia–Ukraine conflict on food security in the Middle East and North Africa (MENA) region is analyzed. With Ukraine being recognized as one of the major global grain producers and exporters, the conflict is seen as posing [...] Read more.
In this article, the impact of the Russia–Ukraine conflict on food security in the Middle East and North Africa (MENA) region is analyzed. With Ukraine being recognized as one of the major global grain producers and exporters, the conflict is seen as posing a significant challenge to MENA countries, which are heavily dependent on grain imports from Ukraine. The importance of global linkages in food supply chains and their influence on regional food security is highlighted in this context. Utilizing secondary data from 2002 to 2021 obtained from the United Nations Food and Agriculture Organization (FAO), the study focuses on demography and food security, analyzing how these factors intertwine with grain export dynamics. The escalating hostilities have disrupted transportation routes, damaged infrastructure, and hindered logistics, resulting in substantial export volume reductions. Geopolitical tensions have exacerbated these effects, diminishing confidence among MENA grain importers. The study highlights how these disruptions have influenced global supply chains, prices, and agricultural product availability, with a specific focus on the MENA region’s challenges in food security, compounded by conflicts, climate change, and import dependence. A detailed demographic analysis reveals the impact of population changes on food demand and distribution, offering insights into how population growth and urbanization, alongside shifts in malnutrition and obesity rates, affect food security. The study concludes that the MENA region’s increasing reliance on food imports, coupled with climatic and political variabilities, underscores its growing vulnerability to global supply chain disruptions and the need for robust strategies to address these challenges. Full article
(This article belongs to the Section Agricultural Economics, Policies and Rural Management)
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<p>Demographic trends in the MENA region from 2001 to 2021: population (<span class="html-italic">y</span><sub>1</sub>); number of undernourished people (<span class="html-italic">y</span><sub>2</sub>); number of obese people over the age of 18 (<span class="html-italic">y</span><sub>3</sub>) with fitted trend lines.</p>
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<p>Cereal production and import in the MENA region from 2001 to 2021, expressed in millions of tons.</p>
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<p>Ukraine’s share in cereal imports to MENA countries from 2001 to 2021.</p>
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<p>Structure of cereal imports from Ukraine to MENA from 2001 to 2021.</p>
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<p>Key indicators in the analysis of demography and food security.</p>
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21 pages, 4727 KiB  
Article
The Influence of Applying Foliar Micronutrients at Nodulation and the Physiological Properties of Common Soybean Plants
by Wacław Jarecki, Tomasz Lachowski and Dagmara Migut
Agriculture 2024, 14(1), 154; https://doi.org/10.3390/agriculture14010154 - 20 Jan 2024
Viewed by 1368
Abstract
Legumes, due to their symbiosis with papillary bacteria, can receive nitrogen from the air. The remaining nutrients must be supplied in fertilisers, either soil or foliar. In the pot experiment, we recorded the responses of two soybean cultivars (Annushka, Pompei) to the foliar [...] Read more.
Legumes, due to their symbiosis with papillary bacteria, can receive nitrogen from the air. The remaining nutrients must be supplied in fertilisers, either soil or foliar. In the pot experiment, we recorded the responses of two soybean cultivars (Annushka, Pompei) to the foliar application of micronutrients (control, Zn, Fe, Cu, Mn, B, or Mo). The physiological properties were expressed as net photosynthetic rate (PN), intercellular CO2 concentration (Ci), transpiration rate (E), stomatal conductance (gs), maximum quantum yield of photosystem II (Fv/Fm), maximum quantum yield of primary photochemistry (Fv/F0), photosynthetic performance index (PI), and the development of soil plant analyses (SPAD), which were analysed. The effects of individual micronutrients on nodulation, plant growth, and condition were also investigated. Micronutrient fertilisation had a positive effect on plant fresh weight and no negative effect on plant condition. It was shown that elements such as B, Fe, and Mo had the most beneficial effect on nodulation compared to the control, regardless of the cultivar analysed. The application of single-component foliar fertilisers improved the physiological parameters of the plants. The relative chlorophyll content was most favourably affected by the application of Mn, B, and Mo in the Annushka cultivar, and Fe, Mn, and Mo in the Pompei cultivar. Similarly, in the case of chlorophyll fluorescence, the most stimulating effect was found for Mn and B, regardless of the cultivar. In the case of gas exchange, the application of Fe, Mo, and B for the Annushka cultivar and Cu for the Pompei cultivar had the most favourable effect on physiological measurements. The results obtained indicate that the foliar application of the evaluated micronutrients is justified in soybean cultivation and does not disturb the nodulation process. Full article
(This article belongs to the Special Issue Foliar Fertilization for Sustainable Crop Production)
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<p>Changes in fresh weight (FW) and plant condition (9—most favourable, 1—least favourable) caused by fertilisation with single-component foliar fertilisers, depending on the element applied.</p>
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<p>Effect of applied foliar fertilisation on the average number of nodules per root per plant. a–c statistically significant differences between values within the cultivar at a confidence level of <span class="html-italic">p</span> = 0.05.</p>
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<p>Effect of applied foliar fertilisation on mean values of relative chlorophyll content in soybean leaves on successive measurement dates. a–b statistically significant differences between values within the date and cultivar at a confidence level of <span class="html-italic">p</span> = 0.05.</p>
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<p>Effect of applied foliar fertilisation on chlorophyll fluorescence parameters: (<b>a</b>) maximum quantum yield of PSII photochemistry (F<sub>v</sub>/F<sub>m</sub>), (<b>b</b>) maximum yield of primary photochemistry (F<sub>v</sub>/F<sub>0</sub>), (<b>c</b>) PS II performance index (PI). a–d statistically significant differences between the values within the term and the cultivar at the confidence level <span class="html-italic">p</span> = 0.05.</p>
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<p>Effect of applied foliar fertilisation on chlorophyll fluorescence parameters: (<b>a</b>) maximum quantum yield of PSII photochemistry (F<sub>v</sub>/F<sub>m</sub>), (<b>b</b>) maximum yield of primary photochemistry (F<sub>v</sub>/F<sub>0</sub>), (<b>c</b>) PS II performance index (PI). a–d statistically significant differences between the values within the term and the cultivar at the confidence level <span class="html-italic">p</span> = 0.05.</p>
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<p>Effect of applied foliar fertilisation on chlorophyll fluorescence parameters: (<b>a</b>) maximum quantum yield of PSII photochemistry (F<sub>v</sub>/F<sub>m</sub>), (<b>b</b>) maximum yield of primary photochemistry (F<sub>v</sub>/F<sub>0</sub>), (<b>c</b>) PS II performance index (PI). a–d statistically significant differences between the values within the term and the cultivar at the confidence level <span class="html-italic">p</span> = 0.05.</p>
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<p>Effect of applied foliar fertilisation on soybean gas exchange parameters: (<b>a</b>) net photosynthetic rate (P<sub>N</sub>), (<b>b</b>) transpiration rate (E), (<b>c</b>) stomatal conductance (g<sub>s</sub>), (<b>d</b>) intercellular CO<sub>2</sub> concentration (C<sub>i</sub>). a–c statistically significant differences between values within a term and at a confidence level of <span class="html-italic">p</span> = 0.05.</p>
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<p>Effect of applied foliar fertilisation on soybean gas exchange parameters: (<b>a</b>) net photosynthetic rate (P<sub>N</sub>), (<b>b</b>) transpiration rate (E), (<b>c</b>) stomatal conductance (g<sub>s</sub>), (<b>d</b>) intercellular CO<sub>2</sub> concentration (C<sub>i</sub>). a–c statistically significant differences between values within a term and at a confidence level of <span class="html-italic">p</span> = 0.05.</p>
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<p>Effect of applied foliar fertilisation on soybean gas exchange parameters: (<b>a</b>) net photosynthetic rate (P<sub>N</sub>), (<b>b</b>) transpiration rate (E), (<b>c</b>) stomatal conductance (g<sub>s</sub>), (<b>d</b>) intercellular CO<sub>2</sub> concentration (C<sub>i</sub>). a–c statistically significant differences between values within a term and at a confidence level of <span class="html-italic">p</span> = 0.05.</p>
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<p>Effect of applied foliar fertilisation on soybean gas exchange parameters: (<b>a</b>) net photosynthetic rate (P<sub>N</sub>), (<b>b</b>) transpiration rate (E), (<b>c</b>) stomatal conductance (g<sub>s</sub>), (<b>d</b>) intercellular CO<sub>2</sub> concentration (C<sub>i</sub>). a–c statistically significant differences between values within a term and at a confidence level of <span class="html-italic">p</span> = 0.05.</p>
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30 pages, 5888 KiB  
Article
Comprehensive Economic Impacts of Wild Pigs on Producers of Six Crops in the South-Eastern US and California
by Sophie C. McKee, John J. Mayer and Stephanie A. Shwiff
Agriculture 2024, 14(1), 153; https://doi.org/10.3390/agriculture14010153 - 20 Jan 2024
Cited by 3 | Viewed by 1671
Abstract
Wild pigs (Sus scrofa) cause damage to agricultural crops in their native range as well as in the portions of the globe where they have been introduced. In the US, states with the highest introduced wild pig populations are Alabama, Arkansas, [...] Read more.
Wild pigs (Sus scrofa) cause damage to agricultural crops in their native range as well as in the portions of the globe where they have been introduced. In the US, states with the highest introduced wild pig populations are Alabama, Arkansas, California, Florida, Georgia, Louisiana, Mississippi, Missouri, North Carolina, South Carolina, and Texas. The present study summarizes the first survey-based effort to value the full extent of wild pig damage to producers of six crops in these eleven US states. The survey was distributed by the USDA National Agricultural Statistical Service in the summer of 2022 to a sample of 11,495 producers of corn (Zea mays), soybeans (Glycine max), wheat (Triticum spp.), rice (Oryza sativa), peanuts (Arachis hypogaea), and sorghum (Sorghum bicolor) in these 11 states. Our findings suggest that the economic burden of wild pigs on producers of these crops is substantial and not limited to the direct and most identifiable categories of crop damage (i.e., production value lost due to depredation, trampling and rooting). We estimate that the annual cost to producers of these six crops in the surveyed states in 2021 was almost USD 700 million. Full article
(This article belongs to the Section Crop Protection, Diseases, Pests and Weeds)
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<p>Fraction of producers reporting wild pig presence in county in the last three years.</p>
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<p>Fraction of producers with wild pig presence in county in the last three years, by state and level of change reported (“eliminated completely” not represented because the share was either zero or subject to disclosure rule).</p>
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<p>Fraction of producers reporting wild pig presence on operation in the last three years.</p>
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<p>Fraction of producers with wild pig presence on operation in the last three years, by state and level of change reported (“eliminated completely” not represented because the share was either zero or subject to disclosure rule).</p>
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<p>Fraction of crop producers reporting changing their crop planting decision in 2021, by sub-group of crop producers based on reported wild pig presence.</p>
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<p>In red: crop not planted or planted less of in 2021 because of wild pigs. In green: crop grown instead.</p>
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<p>Fraction of producers of any of the six studied crops reporting wild pig presence on any field (red), replanting because of wild pig damage (yellow), crop damage by wild pigs (blue), and incurring additional costs at harvest because of wild pigs (green).</p>
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<p>Distribution of mean crop damage ratio across counties with wild pig presence.</p>
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<p>Fraction of producers with wild pig presence in county in the last three years, by state and level of change reported (“University Outreach Services” not represented because the share was either zero or subject to disclosure rule). Totals by state can sum to more than 100%.</p>
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<p>Questions pertaining to wild pig populations.</p>
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<p>Questions pertaining to opportunity costs.</p>
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<p>Questions pertaining to the six study crops.</p>
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<p>Questions pertaining to property damage and the associated hours and money spent on repair.</p>
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<p>Questions pertaining to control methods.</p>
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23 pages, 1605 KiB  
Review
Beneficial Soil Microbiomes and Their Potential Role in Plant Growth and Soil Fertility
by Éva-Boglárka Vincze, Annamária Becze, Éva Laslo and Gyöngyvér Mara
Agriculture 2024, 14(1), 152; https://doi.org/10.3390/agriculture14010152 - 20 Jan 2024
Cited by 6 | Viewed by 5547
Abstract
The soil microbiome plays an important role in maintaining soil health, plant productivity, and soil ecosystem services. Current molecular-based studies have shed light on the fact that the soil microbiome has been quantitatively underestimated. In addition to metagenomic studies, metaproteomics and metatranscriptomic studies [...] Read more.
The soil microbiome plays an important role in maintaining soil health, plant productivity, and soil ecosystem services. Current molecular-based studies have shed light on the fact that the soil microbiome has been quantitatively underestimated. In addition to metagenomic studies, metaproteomics and metatranscriptomic studies that target the functional part of the microbiome are becoming more common. These are important for a better understanding of the functional role of the microbiome and for deciphering plant-microbe interactions. Free-living beneficial bacteria that promote plant growth by colonizing plant roots are called plant growth-promoting rhizobacteria (PGPRs). They exert their beneficial effects in different ways, either by facilitating the uptake of nutrients and synthesizing particular compounds for plants or by preventing and protecting plants from diseases. A better understanding of plant-microbe interactions in both natural and agroecosystems will offer us a biotechnological tool for managing soil fertility and obtaining a high-yield food production system. Full article
(This article belongs to the Section Agricultural Soils)
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<p>Relative abundance of different bacterial communities in different crops (based on Li et al. [<a href="#B13-agriculture-14-00152" class="html-bibr">13</a>], Mahoney et al. [<a href="#B18-agriculture-14-00152" class="html-bibr">18</a>], Rathore et al. [<a href="#B19-agriculture-14-00152" class="html-bibr">19</a>], Ullah et al. [<a href="#B20-agriculture-14-00152" class="html-bibr">20</a>], Edwards et al. [<a href="#B21-agriculture-14-00152" class="html-bibr">21</a>], and Sugiyama et al. [<a href="#B22-agriculture-14-00152" class="html-bibr">22</a>]).</p>
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<p>Plant growth-promoting mechanisms by soil microbes. PGPRs play an important role in plant growth promotion, stress resistance, plant health and protection, phytoremediation, and ISR.</p>
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<p>Indirect and direct mechanisms of biocontrol agents. Indirect methods for biocontrol agents include induced systemic resistance and plant growth-promoting mechanisms. Biocontrol agents directly protect plants through antimicrobial metabolites and bacterial interactions. Arrows indicate the direction of the relationship.</p>
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20 pages, 3373 KiB  
Article
Farming of Medicinal and Aromatic Plants in Italy: Structural Features and Economic Results
by Dario Macaluso, Francesco Licciardo and Katya Carbone
Agriculture 2024, 14(1), 151; https://doi.org/10.3390/agriculture14010151 - 20 Jan 2024
Viewed by 1565
Abstract
In recent years, the primary sector in Italy and elsewhere has been profoundly affected by climate change and a deep economic crisis, mainly linked to stagnating prices and rising production costs. Because of this situation, we are witnessing renewed interest in alternative agricultural [...] Read more.
In recent years, the primary sector in Italy and elsewhere has been profoundly affected by climate change and a deep economic crisis, mainly linked to stagnating prices and rising production costs. Because of this situation, we are witnessing renewed interest in alternative agricultural productions, which are characterized by their resilience and sustainability, including medicinal and aromatic plants (MAPs). This sector is characterized by a certain heterogeneity due to the great variety of species and their wide range of uses. Although these characteristics contribute to the sector’s economic success, they also hinder its study due to commodity complexity and limited data availability. At the farm level, the situation is complicated by the fact that MAP cultivation is often embedded in complex cropping systems, and more rarely, is practiced exclusively or predominantly. In light of these considerations, we concentrated solely on the agricultural phase of the supply chain, using data available in the Farm Accountancy Data Network. We aimed to examine the main structural characteristics and economic outcomes of Italian farms that grow MAP, as well as the profitability of some of the species. To ensure accurate species classification, only MAPs exclusively designated for botanical use in the Italian National List were considered. The analysis of farm economic performance indicators (gross output, variable costs, gross margins, etc.) focused mainly on the species most represented in the sample: saffron, rosemary, lavender, oregano, and sage. The results indicate that the total gross output and gross margin show the best performance in the case of saffron (66,200 and 57,600 EUR/ha, respectively) and rosemary (27,500 and 22,000 EUR/ha, respectively). However, for saffron, the biggest cost concerns propagation (purchase of bulbs), amounting to 50% of the variable costs, whereas fertilization ones are particularly high for sage and rosemary. Full article
(This article belongs to the Section Agricultural Economics, Policies and Rural Management)
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<p>Number of farms with medicinal and aromatic plants and Utilized Agricultural Area (2019 campaign) (source: own elaboration on AGEA data).</p>
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<p>Distribution of the Utilized Agricultural Area under medicinal and aromatic plants in Italy (2019, values in %) (source: own elaboration on AGEA data). Notes: data for Valle d’Aosta and Trentino-Alto Adige are negligible.</p>
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<p>Difference between the observed frequencies in the sample of farms with MAPs and the relative expected frequencies (source: own elaboration of FADN data).</p>
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<p>Distribution of companies observed by TF (years 2015–2020, values in%) (source: own elaboration on FADN data).</p>
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<p>Farms that practice agriculture-related activities with and without the cultivation of medicinal and aromatic plants (2015–2020, values in %) (source: own elaboration on FADN data).</p>
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<p>Distribution of observed companies by economic size class and farm profile (2015–2020, values in %) (source: elaboration of FADN data).</p>
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<p>FNI per hectare of UAA by farm size (years 2015–2020, values in EUR per hectare) (source: own elaboration on FADN data).</p>
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<p>FNI per worker unit by farm size (years 2015–2020, values in EUR per hectare) (source: elaboration of FADN data).</p>
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<p>TGO and GM values (years 2015–2020, values in EUR per hectare) (source: own elaboration on FADN data).</p>
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<p>Incidence of GM and VCs on TGO (years 2015–2020, values in%) (source: own elaboration on FADN data).</p>
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<p>Incidence of OM and farm labor costs on TGO (years 2015–2020, values in%) (source: own elaboration on FADN data).</p>
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14 pages, 1736 KiB  
Article
Application of a Mechanistic Model to Explore Management Strategies for Biological Control of an Agricultural Pest
by Madeleine G. Barton, Hazel Parry, Paul A. Umina, Matthew R. Binns, Thomas Heddle, Ary A. Hoffmann, Joanne Holloway, Dustin Severtson, Maarten Van Helden, Samantha Ward, Rachel Wood and Sarina Macfadyen
Agriculture 2024, 14(1), 150; https://doi.org/10.3390/agriculture14010150 - 19 Jan 2024
Viewed by 1183
Abstract
Despite the known benefits of integrated pest management, adoption in Australian broadacre crops has been slow, in part due to the lack of understanding about how pests and natural enemies interact. We use a previously developed process-based model to predict seasonal patterns in [...] Read more.
Despite the known benefits of integrated pest management, adoption in Australian broadacre crops has been slow, in part due to the lack of understanding about how pests and natural enemies interact. We use a previously developed process-based model to predict seasonal patterns in the population dynamics of a canola pest, the green peach aphid (Myzus persicae), and an associated common primary parasitic wasp (Diaeretiella rapae), found in this cropping landscape. The model predicted aphid population outbreaks in autumn and spring. Diaeretiella rapae was able to suppress these outbreaks, but only in scenarios with a sufficiently high number of female wasps in the field (a simulated aphid:wasp density ratio of at least 5:1 was required). Model simulations of aphid-specific foliar pesticide applications facilitated biological control. However, a broad-spectrum pesticide negated the control provided by D. rapae, in one case leading to a predicted 15% increase in aphid densities compared to simulations in which no pesticide was applied. Biological and chemical control could therefore be used in combination for the successful management of the aphid while conserving the wasp. This modelling framework provides a versatile tool for further exploring how chemical applications can impact pests and candidate species for biological control. Full article
(This article belongs to the Special Issue Ecosystem Services and Biological Control in Agroecosystems)
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<p>Schematic of the aphid—wasp model with climate and host-plant inputs. Black: apterous (wingless) aphids, mid-grey: alate (winged) aphids; light grey: wasp, red: interactions between the two species through parasitism of second and third instar nymphs. White ovals show (daily) climate inputs used in the APSIM canola model (white circles) and the insect models (dashed rectangle). Days of the model simulation (t) on which immigrating wasps intercept the aphid population are user defined. Reprinted from [<a href="#B26-agriculture-14-00150" class="html-bibr">26</a>].</p>
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<p>Baseline simulations of <span class="html-italic">Myzus persicae</span> population growth dynamics in the absence of chemical or biological control, where (<b>a</b>) aphids arrive on the day of crop emergence for early, middle, and late time of sowing (TOS, <a href="#agriculture-14-00150-t001" class="html-table">Table 1</a>) at South Australian (SA) and Victorian (VIC) sites. Total aphid densities (<b>b</b>) of the population (area under the population curves) varied depending on location, TOS, and delay of aphid arrival into the crop, where week 1 refers to aphids arriving on the day of seedling emergence. Shading and error bars denote variation among the 10-year (climate) replicates (2005–2014).</p>
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<p>Efficacy of control exerted by varying densities of <span class="html-italic">Diaeretiella rapae</span> on <span class="html-italic">Myzus persicae</span>, compared to simulations with no biological control. Here, an efficacy of 100% reflects complete suppression of the aphid population, while 0% reflects aphid densities in a system without <span class="html-italic">any</span> wasps (see <a href="#agriculture-14-00150-f002" class="html-fig">Figure 2</a>). Scenarios for early (<b>a</b>,<b>d</b>), middle (<b>b</b>,<b>e</b>) and late (<b>c</b>,<b>f</b>) TOS at South Australian (<b>a</b>–<b>c</b>) and Victorian (<b>d</b>–<b>f</b>) sites were considered. Error bars denote variation among the 10-year (climate) replicates (2005–2014). Note that the scenario with 10 wasps (yellow squares) is equivalent to that with 5 wasps (light-blue asterisks, both achieved near-full control).</p>
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<p>Impacts of different management strategies on the population dynamics of <span class="html-italic">Myzus persicae</span> throughout the canola growing season with an (<b>a</b>) early, (<b>b</b>) middle, and (<b>c</b>) late sowing time at the South Australian site (where aphids arrive on the day of crop emergence; see <a href="#agriculture-14-00150-f002" class="html-fig">Figure 2</a>a). Management scenarios include no control (NC), biological control (BC), aphid-specific chemical control (ASC) and broad-spectrum chemical control (BSC). The proportion of parasitised aphids (wasp larvae/[wasp larvae + aphids]) on the day of crop maturation for the three sowing times (<b>d</b>–<b>f</b>) provides a measure of the relative success of the management approaches (black points are outlying values below 1.5 × inter-quartile range). Shaded regions (<b>a</b>–<b>c</b>) and box-plot whiskers (<b>d</b>–<b>f</b>) denote standard error of mean and the 75% interquartile range, respectively, where variation is generated from the 10-year (climate) replicates (see <a href="#agriculture-14-00150-t001" class="html-table">Table 1</a> for further details).</p>
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16 pages, 2808 KiB  
Article
Improving Walnut Images Segmentation Using Modified UNet3+ Algorithm
by Jun Tie, Weibo Wu, Lu Zheng, Lifeng Wu and Ting Chen
Agriculture 2024, 14(1), 149; https://doi.org/10.3390/agriculture14010149 - 19 Jan 2024
Cited by 1 | Viewed by 1336
Abstract
When aiming at the problems such as missed detection or misdetection of recognizing green walnuts in the natural environment directly by using target detection algorithms, a method is proposed based on improved UNet3+ for green walnut image segmentation, which incorporates the channel and [...] Read more.
When aiming at the problems such as missed detection or misdetection of recognizing green walnuts in the natural environment directly by using target detection algorithms, a method is proposed based on improved UNet3+ for green walnut image segmentation, which incorporates the channel and spatial attention mechanism CBAM (convolutional block attention module) and cross-entropy loss function (cross-entropy loss) into the UNet3+ network structure, and introduces the five-layer CBAM in the encoder module to construct the improved UNet3+ network model. The model consists of an encoder module (down-sampling), a decoder module (up-sampling) and a full-scale skip connection module, a full-scale feature supervision module, and a classification guidance module. After utilizing data-enhanced approaches to expand the green walnut dataset, the improved UNet3+ model was trained. The experimental findings demonstrate that the improved UNet3+ network model achieves 91.82% average precision, 96.00% recall rate, and 93.70% F1 score in the green walnut segmentation task; the addition of five-layer CBAM boosts the model segmentation precision rate by 3.11 percentage points. The method can precisely and successfully segment green walnuts, which can serve as a guide and research foundation for precisely identifying and localizing green walnuts and finishing the autonomous sorting for intelligent robots. Full article
(This article belongs to the Section Digital Agriculture)
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<p>Original image of green walnut.</p>
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<p>Image of green walnut after data enhancement.</p>
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<p>UNet3+ network structure.</p>
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<p>Classification guidance module.</p>
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<p>Improved UNet3+ network architecture.</p>
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<p>CBAM network architecture.</p>
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<p>Channel attention module.</p>
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<p>Spatial attention module.</p>
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<p>Segmentation effect of green walnut with different network models.</p>
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<p>Curve of IOU values with training rounds.</p>
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14 pages, 3551 KiB  
Article
Calibration of Simulation Parameters for Fresh Tea Leaves Based on the Discrete Element Method
by Dongdong Li, Rongyang Wang, Yingpeng Zhu, Jianneng Chen, Guofeng Zhang and Chuanyu Wu
Agriculture 2024, 14(1), 148; https://doi.org/10.3390/agriculture14010148 - 19 Jan 2024
Cited by 2 | Viewed by 1181
Abstract
To address the problem of a lack of accurate parameters in the discrete element simulation study of the machine-picked fresh tea leaf mechanized-sorting process, this study used machine-picked fresh tea leaves as the research object, established discrete element models of different fresh tea [...] Read more.
To address the problem of a lack of accurate parameters in the discrete element simulation study of the machine-picked fresh tea leaf mechanized-sorting process, this study used machine-picked fresh tea leaves as the research object, established discrete element models of different fresh tea leaf components in EDEM software version 7.0.0. based on the bonded particle model using three-dimensional scanning inverse-modeling technology, and calibrated the simulation parameters through physical tests and virtual simulation tests. Firstly, the intrinsic parameters of machine-picked tea leaves were measured using physical tests; the physical-stacking tea leaf test was conducted using the cylinder lifting method, the tea leaf repose angle being 32.62° as measured from the stacking images using CAD. With the physical repose angle as the target value, the Plackeet–Burman test, the steepest-ascent test and the Box–Behnken optimization test were conducted in turn, and the results showed that the static friction coefficient between tea leaves, the rolling friction coefficient between tea leaves and the static friction coefficient between tea leaves and PVC have a major effect on the repose angle, and the optimal combination of the three significant parameters was determined. Finally, five simulations were conducted using the optimal combination of parameters, the relative error between the repose angle measured by the simulation test and the physical repose angle being just 0.28%. Moreover, the t-test obtained p > 0.05, indicating that there was no significant difference between the simulation test results and the physical test results. The results showed that the calibrated discrete element simulation parameters obtained could provide a reference for the discrete element simulation study of fresh tea leaves. Full article
(This article belongs to the Section Agricultural Technology)
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<p>Geometric dimensions of fresh tea leaves: (<b>a</b>) leaf length (L) and leaf width (W); (<b>b</b>) leaf thickness (T).</p>
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<p>Density measurement of fresh tea leaves: (<b>a</b>) quality measurement of tea leaves; (<b>b</b>) volume measurement of tea leaves.</p>
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<p>Mass Spectrometer. 1—compression probe, 2—fresh leaf stem, 3—carrier table.</p>
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<p>Physical repose angle test of fresh tea leaves.</p>
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<p>The acquisition process of a fresh tea leaf 3D model.</p>
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<p>Discrete element model of fresh tea leaves: (<b>a</b>) single bud; (<b>b</b>) one bud and one leaf; (<b>c</b>) one bud and two leaves.</p>
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<p>BondingV2 model of fresh tea leaves: (<b>a</b>) single bud (<b>b</b>) one bud and one leaf (<b>c</b>) one bud and two leaves.</p>
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<p>Simulation test of the repose angle.</p>
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<p>Measurement of repose angle in simulation test: (<b>a</b>) tea leaf stacking +X direction, (<b>b</b>) tea leaf stacking +Y direction.</p>
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<p>Effect of the interaction term on the repose angle: (<b>a</b>) interactive effects of <span class="html-italic">X<sub>2</sub></span> and <span class="html-italic">X<sub>3</sub></span>; (<b>b</b>) interactive effects of <span class="html-italic">X<sub>3</sub></span> and <span class="html-italic">X<sub>5</sub></span>.</p>
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25 pages, 610 KiB  
Article
Impacts of Capital Endowment on Farmers’ Choices in Fertilizer-Reduction and Efficiency-Increasing Technologies (Preferences, Influences, and Mechanisms): A Case Study of Apple Farmers in the Provinces of Shaanxi and Gansu, China
by Yihan Chen, Wen Xiang and Minjuan Zhao
Agriculture 2024, 14(1), 147; https://doi.org/10.3390/agriculture14010147 - 19 Jan 2024
Cited by 1 | Viewed by 1439
Abstract
On the basis of data collected from 1208 apple farmers in the provinces of Shaanxi and Gansu, this study utilizes the weighted-frequency method to investigate the priority sequence of farmers’ preferences in choosing fertilizer-reduction and efficiency-increasing technologies. Subsequently, ordered-probit models, a mediating-effect model, [...] Read more.
On the basis of data collected from 1208 apple farmers in the provinces of Shaanxi and Gansu, this study utilizes the weighted-frequency method to investigate the priority sequence of farmers’ preferences in choosing fertilizer-reduction and efficiency-increasing technologies. Subsequently, ordered-probit models, a mediating-effect model, and a moderating-effect model are employed to empirically analyze the influence of capital endowment on farmers’ choices related to fertilizer-reduction and efficiency-increasing technologies and their underlying mechanisms. The study further examines how agricultural-technology extension moderates these mechanisms. The main findings are: (1) The priority sequence of farmers’ choices concerning fertilizer-reduction and efficiency-increasing technologies is as follows: organic fertilizer substitution, new efficient fertilizers, soil testing and formula fertilization, green manure cultivation, straw mulching, fertilizer-reduction application, and deep mechanical application. (2) Capital endowment significantly enhances farmers’ choices in fertilizer-reduction and efficiency-increasing technologies. (3) The mechanism analyses indicate that capital endowment can promote farmers’ choices in fertilizer-reduction and efficiency-increasing technologies by improving their information-acquisition capabilities. (4) Moderation effects reveal that agricultural-technology extension methods, such as technical training, financial subsidies, and government publicity, significantly and positively moderate the relationship between information-acquisition capabilities and farmers’ choices in fertilizer-reduction and efficiency-increasing technologies. The moderating effects of educational attainment and generational differences on different agricultural-technology extension methods are heterogeneous. Technical training, financial subsidies, and government publicity can effectively enhance the positive impact of information-acquisition capabilities on farmers with a higher educational attainment. Financial subsidies can effectively strengthen the positive impact of information-acquisition capabilities on the older generation of farmers. Therefore, it is recommended to prioritize the accumulation of farmers’ capital endowment, improve their information-acquisition capabilities, and intensify agricultural-technology extension efforts, especially taking into account farmers’ educational attainment and generational differences. Full article
(This article belongs to the Special Issue Agricultural Policies toward Sustainable Farm Development)
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<p>Theoretical framework of capital endowment on farmers’ selection of fertilizer-reduction and efficiency-increasing technologies.</p>
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18 pages, 5287 KiB  
Article
Development and Experimentation of Intra-Row Weeding Device for Organic Rice
by Jinkang Jiao, Lian Hu, Gaolong Chen, Chaowen Chen and Ying Zang
Agriculture 2024, 14(1), 146; https://doi.org/10.3390/agriculture14010146 - 19 Jan 2024
Cited by 1 | Viewed by 1166
Abstract
Weeds in paddy fields can seriously reduce rice yield. An intra-row weeding device with double-layer elastic rods was designed, considering the differences in mechanical properties between rice and weeds, which can press weeds into the soil and avoid damaging rice. The elastic force [...] Read more.
Weeds in paddy fields can seriously reduce rice yield. An intra-row weeding device with double-layer elastic rods was designed, considering the differences in mechanical properties between rice and weeds, which can press weeds into the soil and avoid damaging rice. The elastic force of the elastic rods can be adjusted by changing the position of the regulating mechanism to adapt to different weeding conditions. A measurement experiment was conducted to determine the variation rule of elastic force. The quadratic orthogonal rotation combination discrete element simulation experiment, which used weeding depth and weeding speed as experimental factors, and the amount of soil disturbance and the force of the inner and outer elastic rod in the horizontal and vertical directions as experimental indicators, was conducted to study the interaction between the weeding device and the soil. The optimal weeding parameters were obtained: the weeding depth was 15 mm, the weeding speed was 0.9 m/s. The field experiment, which used the various parameters of the weeding device as experimental factors and the weeding rate and damaging seedling rate as experimental indicators, was conducted to determine the weeding effect. The experimental results showed that the optimal position of the regulating mechanism was 270 mm, with a weeding rate of 80.65% and a damaging seedling rate of 3.36%. The weeding rate can be increased by at least 11.18% by adjusting the regulating mechanism to a suitable position under the same weeding conditions. This study can provide a reference for research on weeding machinery for organic rice. Full article
(This article belongs to the Section Agricultural Technology)
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<p>Schematic diagram of the intra-row weeding device: (1) connecting frame; (2) frame; (3) installing frame; (4) elastic regulating mechanism; (5) fixing plate; (6) outer elastic rods; (7) inner elastic rods; (8) U-bolt.</p>
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<p>Mechanical analysis of the weeding device to weeds or rice in vertical direction: (<b>a</b>) horizontal; (<b>b</b>) obliquely upward; (<b>c</b>) obliquely upward. <span class="html-italic">O</span> is the action point of the weeding device to weeds or rice. <span class="html-italic">F</span>′ is the force of the elastic rods to weeds or rice, N; <span class="html-italic">F</span><sub>1</sub>′ and <span class="html-italic">F</span><sub>2</sub>′ are the component forces of <span class="html-italic">F</span>′ in horizontal and vertical directions, N; <span class="html-italic">β</span> is the angle between <span class="html-italic">F</span> and the horizontal plane, deg; <span class="html-italic">F<sub>f</sub></span> is the force of the soil resistance, N; <span class="html-italic">F<sub>b</sub></span> is the maximum static resistance force of rice roots, N.</p>
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<p>Mechanical analysis of the weeding device acting on weeds and rice in a horizontal direction: (1) intra-row weeding device; (2) weeds; (3) rice; <span class="html-italic">F</span><sub>1</sub>, <span class="html-italic">F</span><sub>2</sub> are the forces of the elastic rods acting on weeds or rice, N; <span class="html-italic">F</span> is the resultant force of <span class="html-italic">F</span><sub>1</sub> and <span class="html-italic">F</span><sub>2</sub>, N; <span class="html-italic">F<sub>c</sub></span>, <span class="html-italic">F<sub>c</sub></span><sub>1</sub>, and <span class="html-italic">F<sub>c</sub></span><sub>2</sub> are the forces of weeds acting on the elastic rods, N; <span class="html-italic">F<sub>s</sub></span><sub>1</sub> and <span class="html-italic">F<sub>s</sub></span><sub>2</sub> are the forces of rice acting on the elastic rods, N; <span class="html-italic">F<sub>f</sub></span>, <span class="html-italic">F<sub>f</sub></span><sub>1</sub> and <span class="html-italic">F<sub>f</sub></span><sub>2</sub> are the forces of the soil resistance, N.</p>
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<p>Mechanical analysis of the weeding device to soil in vertical direction: (1) intra-row weeding device; (2) weed; (4) paddy soil; <span class="html-italic">F<sub>y</sub></span> is the pressure of the weeding device to soil, N; <span class="html-italic">F<sub>N</sub></span> is the support force of soil to the weeding device, N.</p>
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<p>Experiment for elastic force of the elastic rod.</p>
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<p>Simulation experiment model of intra-row weeding device.</p>
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<p>Mechanical weeder for organic rice.</p>
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<p>Elastic forces of the elastic rods at the end in the horizontal direction: (<b>a</b>) the position of the regulating mechanism was 420 mm; (<b>b</b>) the position of the regulating mechanism was 370 mm; (<b>c</b>) the position of the regulating mechanism was 320 mm; (<b>d</b>) the position of the regulating mechanism was 270 mm; (<b>e</b>) the position of the regulating mechanism was 220 mm.</p>
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<p>Elastic forces of the elastic rods at the end in the horizontal direction: (<b>a</b>) the position of the regulating mechanism was 420 mm; (<b>b</b>) the position of the regulating mechanism was 370 mm; (<b>c</b>) the position of the regulating mechanism was 320 mm; (<b>d</b>) the position of the regulating mechanism was 270 mm; (<b>e</b>) the position of the regulating mechanism was 220 mm.</p>
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<p>Elastic forces of the elastic rod at the end in the vertical direction.</p>
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<p>Simulation effect of the intra-row weeding device: (<b>a</b>) the weeding speed was slower; (<b>b</b>) the weeding speed was faster; (<b>c</b>) the weeding depth was shallower; (<b>d</b>) the weeding depth was deeper.</p>
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<p>Simulation effect of the intra-row weeding device: (<b>a</b>) the weeding speed was slower; (<b>b</b>) the weeding speed was faster; (<b>c</b>) the weeding depth was shallower; (<b>d</b>) the weeding depth was deeper.</p>
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<p>Response surface diagrams: (<b>a</b>) amount of soil disturbance; (<b>b</b>) forward resistance of the inner elastic rod; (<b>c</b>) force in the vertical direction of the inner elastic rod; (<b>d</b>) forward resistance of the outer elasticthed; (<b>e</b>) force in the vertical direction of the outer elastic rod.</p>
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<p>Response surface diagrams: (<b>a</b>) amount of soil disturbance; (<b>b</b>) forward resistance of the inner elastic rod; (<b>c</b>) force in the vertical direction of the inner elastic rod; (<b>d</b>) forward resistance of the outer elasticthed; (<b>e</b>) force in the vertical direction of the outer elastic rod.</p>
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<p>Field operation of mechanical weeder for organic rice.</p>
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<p>Field operation of mechanical weeder in paddy field: (<b>a</b>) weaker rice; (<b>b</b>) stronger rice.</p>
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16 pages, 4657 KiB  
Article
Temperature Prediction of Mushrooms Based on a Data—Physics Hybrid Approach
by Mingfei Wang, Xiangshu Kong, Feifei Shan, Wengang Zheng, Pengfei Ren, Jiaoling Wang, Chunling Chen, Xin Zhang and Chunjiang Zhao
Agriculture 2024, 14(1), 145; https://doi.org/10.3390/agriculture14010145 - 19 Jan 2024
Viewed by 1390
Abstract
Temperature has a significant impact on the production of edible mushrooms. The industrial production of edible mushrooms is committed to accurately maintaining the temperature inside the mushroom room within a certain range to achieve quality and efficiency improvement. However, current environmental regulation methods [...] Read more.
Temperature has a significant impact on the production of edible mushrooms. The industrial production of edible mushrooms is committed to accurately maintaining the temperature inside the mushroom room within a certain range to achieve quality and efficiency improvement. However, current environmental regulation methods have problems such as lagging regulation and a large range of temperature fluctuations. There is an urgent need to accurately predict the temperature of mushroom houses in the future period to take measures in advance. Therefore, this article proposes a temperature prediction model for mushroom houses using a data–physical hybrid method. Firstly, the Boruta-SHAP algorithm was used to screen out the key influencing factors on the temperature of the mushroom room. Subsequently, the indoor temperature was decomposed using the optimized variational modal decomposition. Then, the gated recurrent unit neural network and attention mechanism were used to predict each modal component, and the mushroom house heat balance equation was incorporated into the model’s loss function. Finally, the predicted values of each component were accumulated to obtain the final result. The results demonstrated that integrating a simplified physical model into the predictive model based on data decomposition led to a 12.50% reduction in the RMSE of the model’s predictions compared to a purely data-driven model. The model proposed in this article exhibited good predictive performance in small datasets, reducing the time required for data collection in modeling. Full article
(This article belongs to the Section Digital Agriculture)
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<p>Prediction process of mushroom temperature based on the data–physics hybrid model.</p>
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<p>The raw dataset of the mushroom house environment.</p>
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<p>The impact of each feature on the model output.</p>
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<p>Comparison of R<sup>2</sup> values based on different decomposition modes: (<b>a</b>) model based on EMD decomposition; (<b>b</b>) model based on EEMD decomposition; (<b>c</b>) model based on EWT decomposition; (<b>d</b>) model based on VMD decomposition.</p>
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<p>Comparison of R<sup>2</sup> values based on different decomposition modes: (<b>a</b>) model based on EMD decomposition; (<b>b</b>) model based on EEMD decomposition; (<b>c</b>) model based on EWT decomposition; (<b>d</b>) model based on VMD decomposition.</p>
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<p>Comparison of predictive performance of models based on different decomposition modes: (<b>a</b>) comparison of RMSE values predicted by models under four decomposition modes; (<b>b</b>) comparison of training time for model prediction under four decomposition modes.</p>
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<p>Comparison of R<sup>2</sup> values based on different decomposition modes: (<b>a</b>) model based on T0 Dataset; (<b>b</b>) model based on T1 Dataset; (<b>c</b>) model based on T2 Dataset; (<b>d</b>) model based on T3 Dataset.</p>
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<p>Comparison of predictive performance of models based on different datasets: (<b>a</b>) comparison of RMSE values predicted by models under four datasets; (<b>b</b>) comparison of training time for model prediction under four datasets.</p>
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<p>Comparison of R<sup>2</sup> values based on different prediction modes: (<b>a</b>) GRU Model; (<b>b</b>) GRU–A Model; (<b>c</b>) GRU–PHY Model; (<b>d</b>) GRU-A-PHY Model; (<b>e</b>) SSA-VMD-GRU-A Model; (<b>f</b>) SSA-VMD-GRU-A-PHY Model.</p>
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<p>Comparison of <span class="html-italic">RMSE</span> values based on different prediction modes.</p>
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11 pages, 1543 KiB  
Article
Effect of Mussel Shells as Soil pH Amendment on the Growth and Productivity of Rosemary (Rosmarinus officinalis L.) Cultivation
by Alexios Lolas, Aikaterini Molla, Konstantinos Georgiou, Chrysoula Apostologamvrou, Alexandra Petrotou and Konstantinos Skordas
Agriculture 2024, 14(1), 144; https://doi.org/10.3390/agriculture14010144 - 18 Jan 2024
Cited by 2 | Viewed by 3070
Abstract
Mussel shells, with their calcium carbonate content, serve as a natural pH buffer, aiding in neutralizing acidic soils and, consequently, enhancing nutrient availability for plants. The aim of this study was to evaluate the effect of treating soils with mussel shells as a [...] Read more.
Mussel shells, with their calcium carbonate content, serve as a natural pH buffer, aiding in neutralizing acidic soils and, consequently, enhancing nutrient availability for plants. The aim of this study was to evaluate the effect of treating soils with mussel shells as a soil pH amendment on the agronomic characteristics and productivity of Rosmarinus officinalis. A pot experiment was set up for two growing years. The treatments were amended using different doses of mussel shells. Overall, the treatments were the following: C: unamended soil (control); T1: 0.1%; T2: 0.3%; T3: 0.5%; T4: 1%; T5: 3%; T6: 6%. Plant height was higher in pots amended with 6% mussel shells and reached the value of 32.2 cm in the first year and 51 cm in the second. The application of mussel shells increased the branch length by 53.4–58.7% and the number of branches per plant by 61.3–62% in T6 compared to the control. The total yield of fresh and dry weight in the 1st and 2nd year was ordered as follows: T6 > T5 > T4 > T3 > T2 > T1 > C. In conclusion, while the established optimal quantity for neutralizing soil pH is 300 g of mussel shells per 10 kg of soil, it has been observed that a ratio of 600 g of mussel shells proves more effective in terms of both the productivity and agronomic characteristics of rosemary. Full article
(This article belongs to the Special Issue Advances in Medicinal and Aromatic Plants)
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<p>Air temperature and total precipitation during the studied period (April 2022–October 2023).</p>
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<p>Plant height (cm) during the first growing year under the different soil types. Different lowercase letters represent statistical differences between treatments, <span class="html-italic">p &lt;</span> 0.05.</p>
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<p>Plant height (cm) during the second growing year under the different soil types. Different lowercase letters represent statistical differences between treatments, <span class="html-italic">p &lt;</span> 0.05.</p>
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16 pages, 2491 KiB  
Article
Nutritional Value of Parsley Roots Depending on Nitrogen and Magnesium Fertilization
by Elżbieta Wszelaczyńska, Jarosław Pobereżny, Katarzyna Gościnna, Katarzyna Retmańska and Wojciech Jan Kozera
Agriculture 2024, 14(1), 143; https://doi.org/10.3390/agriculture14010143 - 18 Jan 2024
Viewed by 1710
Abstract
Parsley is an herb/vegetable rich in nutritional compounds such as carbohydrates, vitamins, protein, crude fiber, minerals (especially potassium), phosphorus, magnesium, calcium, iron, and essential oils. Limited information is available in the literature on the quality of parsley roots depending on the cultivation technology [...] Read more.
Parsley is an herb/vegetable rich in nutritional compounds such as carbohydrates, vitamins, protein, crude fiber, minerals (especially potassium), phosphorus, magnesium, calcium, iron, and essential oils. Limited information is available in the literature on the quality of parsley roots depending on the cultivation technology used in the form of macronutrients and micronutrients, preparations to stimulate plant growth and development, as well as plant-protection products. A three-year study was undertaken to determine the effect of applying mineral fertilization with nitrogen, including magnesium on the nutritional value of parsley roots in terms of the content of ascorbic acid, total and reducing sugars, and minerals: (total N, K, Mg, Ca). The research material was the root of Petroselinum crispum ssp. tuberosum from an experiment where nitrogen was applied in soil at (0, 40, 80, 120 kg N ha−1) and magnesium at (0, 30 kg MgO ha−1). Nitrogen fertilization increased the nutritional value in terms of total and reducing sugars, as well as total N and Ca content. Applied magnesium fertilization caused a significant increase in the content of all tested nutrients. The most total sugars (127.7 g kg−1 f. m.), reducing sugars (16.8 g kg−1 f. m.), and total N (12.13 g kg−1 d. m.) were accumulated by roots from the object where nitrogen was applied at a maximum rate of 120 kg N ha−1, including magnesium. On the other hand, for the content of K (19.09 g kg−1 d. m.) in the roots, a dose of 80 N ha−1 was sufficient. For ascorbic acid (263.2 g kg−1 f. m.) and Ca (0.461 g kg−1 d. m.), a dose of 40 kg N ha−1 with a constant fertilization of 30 kg MgO ha−1 was sufficient. When applying high doses of nitrogen, lower doses of magnesium are recommended. This is sufficient due to the high nutritional value of parsley roots. Due to the worsening magnesium deficiency in soils in recent years, the use of this nutrient in the cultivation of root vegetables is as justified and timely as possible. Quality-assessment studies of root vegetables should be continued with higher amounts of magnesium fertilization. Different ways of applying magnesium in parsley cultivation should also be tested. Full article
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<p>Field experiment with root parsley.</p>
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<p>Scatter plot of Ntot content in parsley roots in relation to nitrogen and magnesium fertilization [g kg<sup>−1</sup> of dry matter]. <sup>1</sup> Mean Ntot content; <sup>2</sup> LSD—least significant difference; <sup>3</sup> Experiment factors: A—nitrogen fertilization doses, B—magnesium fertilization doses.</p>
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<p>Mg content in parsley roots depending on nitrogen and magnesium fertilization [g kg<sup>−1</sup> of dry matter]. <sup>1</sup> Mean Mg content, <sup>2</sup> LSD—least significant difference, N. S.—no significant; <sup>3</sup> Experiment factors: A—nitrogen fertilization doses, B—magnesium fertilization doses.</p>
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<p>K content in parsley roots depending on nitrogen and magnesium fertilization [g kg<sup>−1</sup> of dry matter]. <sup>1</sup> Mean K content, <sup>2</sup> LSD—least significant difference, N. S.—no significant; <sup>3</sup> Experiment factors: A—nitrogen fertilization doses, B—magnesium fertilization doses.</p>
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<p>Ca content in parsley roots depending on nitrogen and magnesium fertilization [g kg<sup>−1</sup> of dry matter]. <sup>1</sup> Mean Ca content, <sup>2</sup> LSD—least significant difference, N. S.—no significant; <sup>3</sup> Experiment factors: A—nitrogen fertilization doses, B—magnesium fertilization doses.</p>
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20 pages, 5753 KiB  
Article
A Dual-Branch Model Integrating CNN and Swin Transformer for Efficient Apple Leaf Disease Classification
by Haiping Si, Mingchun Li, Weixia Li, Guipei Zhang, Ming Wang, Feitao Li and Yanling Li
Agriculture 2024, 14(1), 142; https://doi.org/10.3390/agriculture14010142 - 18 Jan 2024
Cited by 3 | Viewed by 1566
Abstract
Apples, as the fourth-largest globally produced fruit, play a crucial role in modern agriculture. However, accurately identifying apple diseases remains a significant challenge as failure in this regard leads to economic losses and poses threats to food safety. With the rapid development of [...] Read more.
Apples, as the fourth-largest globally produced fruit, play a crucial role in modern agriculture. However, accurately identifying apple diseases remains a significant challenge as failure in this regard leads to economic losses and poses threats to food safety. With the rapid development of artificial intelligence, advanced deep learning methods such as convolutional neural networks (CNNs) and Transformer-based technologies have made notable achievements in the agricultural field. In this study, we propose a dual-branch model named DBCoST, integrating CNN and Swin Transformer. CNNs focus on extracting local information, while Transformers are known for their ability to capture global information. The model aims to fully leverage the advantages of both in extracting local and global information. Additionally, we introduce the feature fusion module (FFM), which comprises a residual module and an enhanced Squeeze-and-Excitation (SE) attention mechanism, for more effective fusion and retention of both local and global information. In the natural environment, there are various sources of noise, such as the overlapping of apple branches and leaves, as well as the presence of fruits, which increase the complexity of accurately identifying diseases on apple leaves. This unique challenge provides a robust experimental foundation for validating the performance of our model. We comprehensively evaluate our model by conducting comparative experiments with other classification models under identical conditions. The experimental results demonstrate that our model outperforms other models across various metrics, including accuracy, recall, precision, and F1 score, achieving values of 97.32%, 97.33%, 97.40%, and 97.36%, respectively. Furthermore, detailed comparisons of our model’s accuracy across different diseases reveal accuracy rates exceeding 96% for each disease. In summary, our model performs better overall, achieving balanced accuracy across different apple leaf diseases. Full article
(This article belongs to the Section Crop Protection, Diseases, Pests and Weeds)
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<p>The selected apple disease category. (<b>a</b>) Frog eye spot; (<b>b</b>) powdery mildew; (<b>c</b>) scab; (<b>d</b>) rust; (<b>e</b>) healthy.</p>
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<p>Image after data enhancement.</p>
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<p>The architecture of DBCoST model.</p>
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<p>The details of residual block and Swin Transformer block. (<b>a</b>) Residual block; (<b>b</b>) Swin Transformer block.</p>
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<p>Illustration of the operation of the window-based attention mechanism and the shift window-based attention mechanism. (<b>a</b>) The window partition process based on W-MSA; (<b>b</b>) The shift window process based on SW-MSA; (<b>c</b>) Window cyclic shift process based on SW-MSA; (<b>d</b>) The results with masks.</p>
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<p>The architecture of FFM.</p>
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<p>The accuracy and loss curves of the DBCoST model. (<b>a</b>) Accuracy curve; (<b>b</b>) loss curve.</p>
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<p>The comparison of accuracy and loss curves among different models. (<b>a</b>) Accuracy curve; (<b>b</b>) Loss curve.</p>
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<p>Visualized results of different models in recognizing apple leaf diseases. The red box indicates the presence of the diseased area (<b>a</b>) Frog eye spot; (<b>b</b>) powdery mildew; (<b>c</b>) rust; (<b>d</b>) scab.</p>
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<p>The comparison of accuracy and loss curves of different schemes. (<b>a</b>) Accuracy curve; (<b>b</b>) Loss curve.</p>
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15 pages, 18746 KiB  
Article
ICNet: A Dual-Branch Instance Segmentation Network for High-Precision Pig Counting
by Shanghao Liu, Chunjiang Zhao, Hongming Zhang, Qifeng Li, Shuqin Li, Yini Chen, Ronghua Gao, Rong Wang and Xuwen Li
Agriculture 2024, 14(1), 141; https://doi.org/10.3390/agriculture14010141 - 18 Jan 2024
Cited by 1 | Viewed by 1218
Abstract
A clear understanding of the number of pigs plays a crucial role in breeding management. Computer vision technology possesses several advantages, as it is harmless and labour-saving compared to traditional counting methods. Nevertheless, the existing methods still face some challenges, such as: (1) [...] Read more.
A clear understanding of the number of pigs plays a crucial role in breeding management. Computer vision technology possesses several advantages, as it is harmless and labour-saving compared to traditional counting methods. Nevertheless, the existing methods still face some challenges, such as: (1) the lack of a substantial high-precision pig-counting dataset; (2) creating a dataset for instance segmentation can be time-consuming and labor-intensive; (3) interactive occlusion and overlapping always lead to incorrect recognition of pigs; (4) existing methods for counting such as object detection have limited accuracy. To address the issues of dataset scarcity and labor-intensive manual labeling, we make a semi-auto instance labeling tool (SAI) to help us to produce a high-precision pig counting dataset named Count1200 including 1220 images and 25,762 instances. The speed at which we make labels far exceeds the speed of manual annotation. A concise and efficient instance segmentation model built upon several novel modules, referred to as the Instances Counting Network (ICNet), is proposed in this paper for pig counting. ICNet is a dual-branch model ingeniously formed of a combination of several layers, which is named the Parallel Deformable Convolutions Layer (PDCL), which is trained from scratch and primarily composed of a couple of parallel deformable convolution blocks (PDCBs). We effectively leverage the characteristic of modeling long-range sequences to build our basic block and compute layer. Along with the benefits of a large effective receptive field, PDCL achieves a better performance for multi-scale objects. In the trade-off between computational resources and performance, ICNet demonstrates excellent performance and surpasses other models in Count1200, AP of 71.4% and AP50 of 95.7% are obtained in our experiments. This work provides inspiration for the rapid creation of high-precision datasets and proposes an accurate approach to pig counting. Full article
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<p>Samples of Count1200 capturing in different situations. (<b>a</b>) Large-scale objects. (<b>b</b>) Normal condition. (<b>c</b>) Small-scale objects. (<b>d</b>) Small-scale objects. (<b>e</b>) Nighttime perspectives. (<b>f</b>) Overlapping and clustering.</p>
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<p>The process and details of SAI.</p>
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<p>Comparison of semi-auto and manual annotation methods. (<b>a</b>) Semi-auto annotation (96 points, 5s per instance). (<b>b</b>) Manual annotation (17 points, 35s per instance).</p>
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<p>Architecture of the model.</p>
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<p>The overall structure of the ICNet, where BN and LN stand for Batch Normalization and Layer Normalization, respectively.</p>
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<p>Illustrating the specific computational components of PDCL.</p>
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<p>Examples displayed under <math display="inline"><semantics> <mrow> <mn>3</mn> <mo>×</mo> <mn>3</mn> </mrow> </semantics></math> kernel within <math display="inline"><semantics> <mrow> <mn>7</mn> <mo>×</mo> <mn>7</mn> </mrow> </semantics></math> image. The pentagram of image represents the center of convolution. The green and blue squares in these two <math display="inline"><semantics> <mrow> <mn>7</mn> <mo>×</mo> <mn>7</mn> </mrow> </semantics></math> images represent the receptive field range of current convolution.</p>
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<p>Visualization results of test set including different situations based on Mask R-CNN. The numbers on pigs of subfigure (<b>a</b>) represent the identifiers of pigs.</p>
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<p>Visualization results of test set including different situations based on InternImage.</p>
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<p>Visualization results of test set including different situations based on ICNet.</p>
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20 pages, 2868 KiB  
Article
Light-YOLO: A Lightweight and Efficient YOLO-Based Deep Learning Model for Mango Detection
by Zhengyang Zhong, Lijun Yun, Feiyan Cheng, Zaiqing Chen and Chunjie Zhang
Agriculture 2024, 14(1), 140; https://doi.org/10.3390/agriculture14010140 - 18 Jan 2024
Cited by 3 | Viewed by 2275
Abstract
This paper proposes a lightweight and efficient mango detection model named Light-YOLO based on the Darknet53 structure, aiming to rapidly and accurately detect mango fruits in natural environments, effectively mitigating instances of false or missed detection. We incorporate the bidirectional connection module and [...] Read more.
This paper proposes a lightweight and efficient mango detection model named Light-YOLO based on the Darknet53 structure, aiming to rapidly and accurately detect mango fruits in natural environments, effectively mitigating instances of false or missed detection. We incorporate the bidirectional connection module and skip connection module into the Darknet53 structure and compressed the number of channels of the neck, which minimizes the number of parameters and FLOPs. Moreover, we integrate structural heavy parameter technology into C2f, redesign the Bottleneck based on the principles of the residual structure, and introduce an EMA attention mechanism to amplify the network’s emphasis on pivotal features. Lastly, the Downsampling Block within the backbone network is modified, transitioning it from the CBS Block to a Multi-branch–Large-Kernel Downsampling Block. This modification aims to enhance the network’s receptive field, thereby further improving its detection performance. Based on the experimental results, it achieves a noteworthy mAP of 64.0% and an impressive mAP0.5 of 96.1% on the ACFR Mango dataset with parameters and FLOPs at only 1.96 M and 3.65 G. In comparison to advanced target detection models like YOLOv5, YOLOv6, YOLOv7, and YOLOv8, it achieves improved detection outcomes while utilizing fewer parameters and FLOPs. Full article
(This article belongs to the Section Digital Agriculture)
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<p>Image example of ACFR Mango dataset.</p>
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<p>The structure of the CA attention mechanism and the EMA attention mechanism. (<b>a</b>) CA attention mechanism structure and (<b>b</b>) EMA attention mechanism structure.</p>
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<p>Light-YOLO model structure.</p>
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<p>BiSC-PAN structure.</p>
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<p>RepConv Block structure. (<b>a</b>) RepConv Block structure during training phase and (<b>b</b>) RepConv Block structure during inference phase.</p>
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<p>Multi-branch–Large-Kernel Downsampling Block structure.</p>
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<p>Residual EMA-Bottleneck structure.</p>
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<p>Three distinct reparameterization structures. (<b>a</b>) Replace the CBS Block below the C2f Block with the RepConv Block. (<b>b</b>) Replace the CBS Block above the C2f Block with the RepConv Block. (<b>c</b>) Replace all CBS Blocks in the C2f Block with the RepConv Block.</p>
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<p>Comparison of PR curves of different lightweight models. (<b>a</b>) YOLOv5-N, (<b>b</b>) YOLOv6-N, (<b>c</b>) YOLOv6-N-DFL, (<b>d</b>) YOLOv7-tiny, (<b>e</b>) YOLOv8-N, and (<b>f</b>) Light-YOLO. The red line represents the value of recall when precision experiences its second decline.</p>
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<p>Comparison of lightweight networks. (<b>a</b>) Relationship curve between mAP and FLOPs and (<b>b</b>) Relationship curve between mAP and parameters.</p>
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<p>Detection results of different lightweight detections in a multiple mango environment. (<b>a</b>) YOLOv5-N; (<b>b</b>) YOLOv6-N-DFL; (<b>c</b>) YOLOv7-Tiny; (<b>d</b>) YOLOv8-N; and (<b>e</b>) Light-YOLO. The green box represents the actual boxes of the mango in the Ground Truth. The red box represents predictive boxes, and the yellow circle represents missed or false detection.</p>
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<p>Detection results of different lightweight detections in a speck mango environment. (<b>a</b>) YOLOv5-N; (<b>b</b>) YOLOv6-N-DFL; (<b>c</b>) YOLOv7-Tiny; (<b>d</b>) YOLOv8-N; and (<b>e</b>) Light-YOLO. The green box represents the actual boxes of the mango in the Ground Truth. The red box represents predictive boxes, and the yellow circle represents missed or false detection.</p>
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<p>Detection results of different lightweight detections in a multiple mango and dark environment. (<b>a</b>) YOLOv5-N; (<b>b</b>) YOLOv6-N-DFL; (<b>c</b>) YOLOv7-Tiny; (<b>d</b>) YOLOv8-N; and (<b>e</b>) Light-YOLO. The green box represents the actual boxes of the mango in the Ground Truth. The red box represents predictive boxes, and the yellow circle represents missed or false detection.</p>
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13 pages, 1497 KiB  
Article
The Efficacy of Plant Pathogens Control by Complexed Forms of Copper
by Monika Grzanka, Łukasz Sobiech, Arkadiusz Filipczak, Jakub Danielewicz, Ewa Jajor, Joanna Horoszkiewicz and Marek Korbas
Agriculture 2024, 14(1), 139; https://doi.org/10.3390/agriculture14010139 - 17 Jan 2024
Cited by 1 | Viewed by 1346
Abstract
Copper is a substance that has been used in plant protection for years. Currently, however, more and more attention is being paid to the need to limit the amount of it that ends up in the natural environment. At the same time, it [...] Read more.
Copper is a substance that has been used in plant protection for years. Currently, however, more and more attention is being paid to the need to limit the amount of it that ends up in the natural environment. At the same time, it is necessary to partially replace synthetic fungicides with alternative preparations. It is therefore worth looking for forms of copper that will contain a smaller amount of the mentioned ingredient while being highly effective. This experiment assessed the effect of selected preparations on the development of mycelium of pathogens of the Fusarium genus and the germination parameters of winter wheat. The efficacy of copper lignosulfonate and copper heptagluconate in seed treatment was tested, comparing them to copper oxychloride, copper hydroxide, and tebuconazole. The obtained results indicate that the use of copper lignosulfonate and copper heptagluconate allows for the effective limitation of the development of the tested pathogens (mycelium development was inhibited by up to 100%). Most of the preparations had no effect on the energy and germination capacity of winter wheat (only in one combination were the values lower than 90%). The use of preparations containing reduced doses of copper is an effective solution when applied as seed dressings. Full article
(This article belongs to the Section Crop Protection, Diseases, Pests and Weeds)
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<p>The effect of preparations containing copper and a synthetic fungicide on the length of winter wheat shoots. 1—control, 2–8—copper lignosulfonate (different doses per 100 kg of grain: 2—0.125 L, 3—0.25 L, 4—0.5 L, 5—1.0 L, 6—1.2 L, 7—1.5 L, 8—3.0 L), 9—copper oxychloride, 10—copper hydroxide, 11—copper heptagluconate, 12—tebuconazole. The doses of preparations of combinations 1–12 are consistent with the numbers and values given in <a href="#agriculture-14-00139-t002" class="html-table">Table 2</a>. Different letters indicate statistically different mean HSD (0.05): uninoculated grain = 0.801 (lower-case letters); inoculated grain = 0.765 (capital letters). Standard deviation: uninoculated grain = 0.557; inoculated grain = 0.532.</p>
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<p>The effect of preparations containing copper and a synthetic fungicide on the length of winter wheat roots. 1<span class="html-italic">—</span>control, 2–8<span class="html-italic">—</span>copper lignosulfonate (different doses per 100 kg of grain: 2<span class="html-italic">—</span>0.125 L, 3<span class="html-italic">—</span>0.25 L, 4<span class="html-italic">—</span>0.5 L, 5<span class="html-italic">—</span>1.0 L, 6<span class="html-italic">—</span>1.2 L, 7<span class="html-italic">—</span>1.5 L, 8<span class="html-italic">—</span>3.0 L), 9<span class="html-italic">—</span>copper oxychloride, 10<span class="html-italic">—</span>copper hydroxide, 11<span class="html-italic">—</span>copper heptagluconate, 12<span class="html-italic">—</span>tebuconazole. The doses of preparations of combinations 1–12 are consistent with the numbers and values given in <a href="#agriculture-14-00139-t002" class="html-table">Table 2</a>. Different letters indicate statistically different mean HSD (0.05): uninoculated grain = 2.614 (lower-case letters); inoculated grain = 2.016 (capital letters). Standard deviation: uninoculated grain = 1.817; inoculated grain = 1.401.</p>
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<p>The effect of preparations containing copper and a synthetic fungicide on the vigor index. 1<span class="html-italic">—</span>control, 2–8—copper lignosulfonate (different doses per 100 kg of grain: 2<span class="html-italic">—</span>0.125 L, 3<span class="html-italic">—</span>0.25 L, 4<span class="html-italic">—</span>0.5 L, 5<span class="html-italic">—</span>1.0 L, 6<span class="html-italic">—</span>1.2 L, 7<span class="html-italic">—</span>1.5 L, 8<span class="html-italic">—</span>3.0 L), 9<span class="html-italic">—</span>copper oxychloride, 10<span class="html-italic">—</span>copper hydroxide, 11<span class="html-italic">—</span>copper heptagluconate, 12<span class="html-italic">—</span>tebuconazole. The doses of preparations of combinations 1–12 are consistent with the numbers and values given in <a href="#agriculture-14-00139-t002" class="html-table">Table 2</a>. Different letters indicate statistically different mean HSD (0.05): uninoculated grain = 331.4 (lower-case letters); inoculated grain = 258.3 (capital letters). Standard deviation: uninoculated grain = 230.389; inoculated grain = 179.538.</p>
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<p>The influence of preparations containing copper and a synthetic fungicide on the infection index. 1—control, 2–8—copper lignosulfonate (different doses per 100 kg of grain: 2—0.125 L, 3—0.25 L, 4—0.5 L, 5—1.0 L, 6—1.2 L, 7—1.5 L, 8—3.0 L), 9—copper oxychloride, 10—copper hydroxide, 11—copper heptagluconate, 12—tebuconazole. The doses of combination preparations 1–12 are consistent with the numbering and values given in <a href="#agriculture-14-00139-t002" class="html-table">Table 2</a>. Different letters indicate statistically different mean HSD (0.05) = 2.1878. Standard deviation = 1.521.</p>
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<p>The influence of preparations containing copper and a synthetic fungicide on the percentage of infection. 1<span class="html-italic">—</span>control, 2–8<span class="html-italic">—</span>copper lignosulfonate (different doses per 100 kg of grain: 2<span class="html-italic">—</span>0.125 L, 3<span class="html-italic">—</span>0.25 L, 4<span class="html-italic">—</span>0.5 L, 5<span class="html-italic">—</span>1.0 L, 6<span class="html-italic">—</span>1.2 L, 7<span class="html-italic">—</span>1.5 L, 8<span class="html-italic">—</span>3.0 L), 9<span class="html-italic">—</span>copper oxychloride, 10<span class="html-italic">—</span>copper hydroxide, 11<span class="html-italic">—</span>copper heptagluconate, 12<span class="html-italic">—</span>tebuconazole. The doses of combination preparations 1–12 are consistent with the numbering and values given in <a href="#agriculture-14-00139-t002" class="html-table">Table 2</a>. Different letters indicate statistically different mean HSD (0.05) = 19.45. Standard deviation = 13.52.</p>
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12 pages, 2115 KiB  
Article
Development of a Seed Treatment with Pochonia chlamydosporia for Biocontrol Application
by Jana Uthoff, Desiree Jakobs-Schönwandt, Karl-Josef Dietz and Anant Patel
Agriculture 2024, 14(1), 138; https://doi.org/10.3390/agriculture14010138 - 17 Jan 2024
Cited by 1 | Viewed by 1720
Abstract
Seed treatment is a powerful technique for adding beneficial ingredients to plants during the seed preparation process. Biopolymers as drying agents and delivery systems in seed treatments were investigated for their biocompatibility with blastospores of the nematophagous fungus Pochonia chlamydosporia. To produce [...] Read more.
Seed treatment is a powerful technique for adding beneficial ingredients to plants during the seed preparation process. Biopolymers as drying agents and delivery systems in seed treatments were investigated for their biocompatibility with blastospores of the nematophagous fungus Pochonia chlamydosporia. To produce a novel seed treatment for the cover crop Phacelia tanacetifolia, xanthan gum TG and gellan gum were the most promising biopolymers in combination with potato starch and bentonite. The seed treatment process as well as the drying process were specially designed to be scalable, which make it suitable for applying the developed seed treatment in agriculture. Application of gellan gum in seed treatments led to 6.3% ± 1.6% of vital blastospores per seed compared to 3.8% ± 0.3% of vital blastospores when applying xanthan gum. Storage tests for seed treatments with 0.5% gellan gum indicated a higher stability at 4 °C compared to storage at 21 °C. After 42 days of storage at 4 °C, 54.1% ± 15.1% of the applied blastospores were viable compared to 0.3% ± 0.8% at 21 °C. This novel seed treatment application with P. chlamydosporia blastospores includes the seed treatment procedure, drying process, and storage tests and can easily be upscaled for application in agriculture. Full article
(This article belongs to the Section Seed Science and Technology)
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<p>Preliminary tests with biopolymers as the drying agent for <span class="html-italic">Pochonia chlamydosporia</span> blastospores. Bar plots show means ± standard deviation for the three biological replicates and the three technical replicates.</p>
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<p>Protective efficiency of xanthan gum TG (XTG), xanthan gum TNAS (XTNAS), and gellan gum (GG) on the viability of <span class="html-italic">Pochonia chlamydosporia</span> blastospores compared to positive control without drying (K+) and dried spores without biopolymer (negative control, K−). Bar plots show mean ± standard deviation for three biological replicates each with three technical replicates. Treatments with different concentrations of the same biopolymer were statistically compared using Kruskal–Wallis test with Dunn’s test for multiple comparisons as post-hoc test (<span class="html-italic">p</span> &lt; 0.05). Different letters indicate significance of difference. n.s. indicates no significant differences between the treatments.</p>
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<p>Survival of <span class="html-italic">Pochonia chlamydosporia</span> blastospores during the seed treatment in the presence of gellan gum and xanthan gum TG. The seed formulation included potato starch and bentonite. Bar plots show mean ± SE for three independent biological replicates of seed treatments with five technical replicates of counting CFU. Different letters indicate significance of difference; n.s. indicates no significant differences between the treatments (Kruskal–Wallis test with Dunn’s test for multiple comparisons as the post-hoc test (<span class="html-italic">p</span> &lt; 0.05)).</p>
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<p>SEM picture of <span class="html-italic">Phacelia tanacetifolia</span>. (<b>A</b>) seeds without coating (5 kV, 23× magnification), (<b>B</b>) treated seeds with bentonite, potato starch, and 0.5% gellan gum (5 kV, 40× magnification). (<b>C</b>) treated seeds with bentonite, potato starch, and 0.5% gellan gum (5 kV, 910× magnification). PC: presumably dried <span class="html-italic">Pochonia chlamydosporia</span> blastospores, B: bentonite, GG: 0.5% gellan gum, PS: potato starch.</p>
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<p>CFU of germinating <span class="html-italic">Pochonia chlamydosporia</span> blastospores after storage tests for seeds treated with bentonite, potato starch, and 0.5% gellan gum at 4 °C and 21 °C. Bar plots show mean ± SD for five technical replicates. Different letters indicate significance of difference (Kruskal–Wallis test with Dunn’s test for multiple comparisons as post-hoc test (<span class="html-italic">p</span> &lt; 0.05)).</p>
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19 pages, 5659 KiB  
Article
Design and Experiment of Active Spiral Pushing Straw Row-Sorting Device
by Zhaoyang Guo, Caiyun Lu, Jin He, Qingjie Wang, Hang Li and Chengkun Zhai
Agriculture 2024, 14(1), 137; https://doi.org/10.3390/agriculture14010137 - 17 Jan 2024
Cited by 1 | Viewed by 1046
Abstract
Aiming to solve the problems of excessive straw residue and large soil loss in the seeding belt of the straw row-sorting operation when the full volume of straw is crushed and returned to the field in the northeastern region of China, an active [...] Read more.
Aiming to solve the problems of excessive straw residue and large soil loss in the seeding belt of the straw row-sorting operation when the full volume of straw is crushed and returned to the field in the northeastern region of China, an active spiral pushing straw row-sorting device (ASPSRD) was designed in this paper. The straw on the surface of the land is collected and stirred by the high-speed rotating spiral-notched blades in the device and pushed to the non-seeding area for the purpose of cleaning the seeding belt. The parameters of key components were determined through theoretical analysis. The orthogonal combination test method of four factors and three levels was adopted. The EDEM discrete element simulation experiment was carried out by selecting the straw mulching quantity (SMQ), the rotating speed of the active rotating shaft (RSARS), the forward speed of the tractor (FST), and the notch width of the spiral notch blade (NWSNB) as the test factors, and the straw-cleaning rate (SCR) and soil loss rate (SLR) as the test indexes. Through range analysis, the parameters were optimized, and the optimal operation parameters of the straw row-sorting device were determined as follows: SMQ was 1.8 kg/m2, RSARS was 120 rpm, FST was 4 m/s, and NWSNB was 9 mm. The operational performance of the device was verified by field experiments. The results of the test showed that after the device was operated with the optimal combination of parameters, the SCR of the 20 cm wide seeding belt was 95.32%, and the SLR was 7.12%, which met the agronomic and technical requirements of corn no-tillage sowing operation in Northeast China. This study can provide a reference for the design of a straw row-sorting device of a no-tillage machine. Full article
(This article belongs to the Section Agricultural Technology)
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<p>The structure diagram of ASPSRD. Note: 1, the active rotating shaft; 2, the left spiral-notched blade group; 3, the right spiral-notched blade group.</p>
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<p>Active spiral push-type straw-cleaning wheel. (<b>a</b>) Main view; (<b>b</b>) left side view. Note: 1, active rotating shaft; 2, right spiral blade group; 3, left spiral blade group.</p>
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<p>Right spiral-notched blade. (<b>a</b>) Main view; (<b>b</b>) left side view. Note: 1, first-stage right spiral pick; 2, second-stage right spiral pick; 3, third-stage right spiral pick; 4, fourth-stage right spiral pick; 5, ungrounded right spiral blade.</p>
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<p>Left spiral-notched blade. (<b>a</b>) Main view; (<b>b</b>) left side view. Note: 1, first-stage left spiral pick; 2, second-stage left spiral pick; 3, third-stage left spiral pick; 4, fourth-stage left spiral pick; 5, ungrounded left spiral blade.</p>
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<p>Layout of spiral picks and notches.</p>
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<p>Simulation model of ASPSRD. Note: 1, simulation model of active spiral push notch straw-cleaning wheel; 2, simulation model of soil; 3, simulation model of straw.</p>
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<p>Straw particle model.</p>
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<p>Calculation area of SCR and SLR in seeding belt.</p>
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<p>Simulation operation process of active spiral push notch straw-cleaning wheel. (<b>a</b>) Early stage of simulation operation; (<b>b</b>) middle stage of simulation operation; (<b>c</b>) later stage of simulation operation.</p>
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<p>Ground straw before operation.</p>
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<p>Field test site.</p>
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<p>Effect of straw cleaning on the seeding belt after operation.</p>
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19 pages, 2257 KiB  
Article
Autonecrotic Tomato (Solanum lycopersicum L.) Line as a Potential Model for Applications in Proximal Sensing of Biotic and Abiotic Stress
by Enrico Santangelo, Angelo Del Giudice, Simone Figorilli, Simona Violino, Corrado Costa, Marco Bascietto, Simone Bergonzoli and Claudio Beni
Agriculture 2024, 14(1), 136; https://doi.org/10.3390/agriculture14010136 - 16 Jan 2024
Viewed by 1278
Abstract
The autonecrotic tomato line V20368 (working code IGSV) spontaneously develops necrotic lesions with acropetal progression in response to an increase in temperature and light irradiation. The process is associated with the interaction between tomato and Cladosporium fulvum, the fungal agent of leaf [...] Read more.
The autonecrotic tomato line V20368 (working code IGSV) spontaneously develops necrotic lesions with acropetal progression in response to an increase in temperature and light irradiation. The process is associated with the interaction between tomato and Cladosporium fulvum, the fungal agent of leaf mold. The contemporary presence of an in-house allele encoding the Rcr3lyc protein and the resistance gene Cf-2pim (from Solanum pimpinellifolium) causes auto-necrosis on the leaves even in the absence of the pathogen (hybrid necrosis). The aim of the work was (i) to examine the potential value of the necrotic genotype as a model system for setting up theoretical guidance for monitoring the phytosanitary status of tomato plants and (ii) to develop a predictive model for the early detection of pathogens (or other stresses) in the tomato or other species. Eighteen IGSV tomato individuals at the 4–5th true-leaf stage were grown in three rows (six plants per row) considered to be replicates. The healthy control was the F1 hybrid Elisir (Olter). A second mutant line (SA410) deriving from a cross between the necrotic mutant and a mutant line of the lutescent (l) gene was used during foliar analysis via microspectrometry. The leaves of the mutants and normal plants were monitored through a portable VIS/NIR spectrometer SCIOTM (Consumer Physics, Tel Aviv, Israel) covering a spectral range between 740 and 1070 nm. Two months after the transplant, the acropetal progression of the autonecrosis showed three symptomatic areas (basal, median, apical) on each IGSV plant: necrotic, partially damaged, and green, respectively. Significantly lower chlorophyll content was found in the basal and median areas of IGSV compared with the control (Elisir). A supervised classification/modelling method (SIMCA) was used. Applying the SIMCA model to the dataset of 162 tomato samples led to the identification of the boundary between the healthy and damaged samples (translational critical distance). Two 10 nm wavelength ranges centred at 865 nm and 1055 nm exhibited a stronger link between symptomatology and spectral reflectance. Studies on specific highly informative mutants of the type described may allow for the development of predictive models for the early detection of pathogens (or other stresses) via proximal sensing. Full article
(This article belongs to the Special Issue Precision Agriculture Technologies for Crop Management)
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<p>Plants of F1 Elisir (<b>A</b>) and IGSV (<b>B</b>) during growth and a particular of the Elisir (<b>C</b>) and IGSV (<b>D</b>) leaves.</p>
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<p>Schematic representation of the methodology adopted for collecting data with the spectrometer SCIO<sup>TM</sup> (Consumer Physics, Tel Aviv, Israel).</p>
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<p>Minimum, average, and maximum temperatures and monthly rainfall registered in the period April–September 2021. Data collected by the Arsial control unit of Monterotondo (RM), location: Grotta Marozza (92 m asl) (<a href="https://www.siarl-lazio.it/E1_2.asp" target="_blank">https://www.siarl-lazio.it/E1_2.asp</a>, accessed 16 November 2023).</p>
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<p>Maximum temperatures (bars) and average daily irradiation (solid line) registered per decade in the period April–September 2021. The dotted line represents the interpolation of the average daily irradiation data. Data collected by the Arsial control unit of Monterotondo (RM), location: Grotta Marozza (92 m asl) (<a href="https://www.siarl-lazio.it/E1_2.asp" target="_blank">https://www.siarl-lazio.it/E1_2.asp</a>, accessed 16 November 2022). The red square indicates the period from transplant to the first appearance of necrotic specks. The red bar indicates the period when the leaves were collected.</p>
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<p>Phenotype of the basal, median, and apical leaflets of IGSV and Elisir F1 at 45 DAT.</p>
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<p>Evolution of autonecroses in the IGSV plants during one month after transplant. The figure reports the height (H) of the plants, the height (H) of the necroses in the plants, and the highest leaf number displaying the necroses (mean ± SD); DAT: Day After Transplant.</p>
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<p>Violin and bar diagram showing the distribution and the amount of the necroses in terms of foliar area (mm<sup>2</sup>) in the basal (BAS) and median leaves (MED) of the IGSV plants (67 DAT). The horizontal line inside the box represents the median.</p>
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<p>Biplot showing the PCA results of IGSV (pink polygon) and Elisir (violet polygon) separation based on the main foliar traits.</p>
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<p>Average curves of spectral reflectance of apical leaf at the stage of 5th (<b>a</b>) and 12fth (<b>b</b>) true-leaf in control (Elisir) and mutant lines (IGSV and SA410).</p>
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<p>Average curves of spectral reflectance of basal, median, and apical leaves of the control (<b>a</b>) and the IGSV mutant (<b>b</b>). Analysis at the stage of 20th true leaf (67 DAT).</p>
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<p>Soft independent modelling by class analogy histogram by frequency class of the SIMCA translational log square performed on the spectral dataset and tested on the mean values for each tomato sample, considering two classes (“healthy leaves_Elisir and IGSV_apical leaves”, “damaged leaves_IGSV” and “defective sample”). The dashed black line represents the critical value (i.e., the model boundary).</p>
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<p>Modelling power obtained by the SIMCA model used to assess healthy plants. The yellow box highlights the most important spectral range that distinguished between healthy plants and sick plants.</p>
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17 pages, 1247 KiB  
Review
Factors Affecting Crop Prices in the Context of Climate Change—A Review
by Huong Nguyen, Marcus Randall and Andrew Lewis
Agriculture 2024, 14(1), 135; https://doi.org/10.3390/agriculture14010135 - 16 Jan 2024
Cited by 3 | Viewed by 3699
Abstract
Food security has become a concerning issue because of global climate change and increasing populations. Agricultural production is considered one of the key factors that affects food security. The changing climate has negatively affected agricultural production, which accelerates food shortages. The supply of [...] Read more.
Food security has become a concerning issue because of global climate change and increasing populations. Agricultural production is considered one of the key factors that affects food security. The changing climate has negatively affected agricultural production, which accelerates food shortages. The supply of agricultural commodities can be heavily influenced by climate change, which leads to climate-induced agricultural productivity shocks impacting crop prices. This paper systematically reviews publications over the past ten years on the factors affecting the prices of a wide range of crops across the globe. This review presents a critical view of these factors in the context of climate change. This paper applies a systematic approach by determining the appropriate works to review with defined inclusion criteria. From this, groups of key factors affecting crop prices are found. This study finds evidence that crop prices have been both positively and negatively affected by a range of factors such as elements of climate change, biofuel, and economic factors. However, the general trend is towards increasing crop prices due to deceasing yields over time. This is the first systematic literature review which provides a comprehensive view of the factors affecting the prices of crops across the world under climate change. Full article
(This article belongs to the Special Issue Application of Computer and Data Analysis in Crop Planning)
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<p>Number of studies on each group of determinants of crop price.</p>
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<p>Locations of studies.</p>
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<p>Factors affecting crop prices in the context of climate change.</p>
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33 pages, 1212 KiB  
Article
Proposal of a Model of Irrigation Operations Management for Exploring the Factors That Can Affect the Adoption of Precision Agriculture in the Context of Agriculture 4.0
by Sergio Monteleone, Edmilson Alves de Moraes, Roberto Max Protil, Brenno Tondato de Faria and Rodrigo Filev Maia
Agriculture 2024, 14(1), 134; https://doi.org/10.3390/agriculture14010134 - 16 Jan 2024
Viewed by 1429
Abstract
Agriculture is undergoing a profound change related to Agriculture 4.0 development and Precision Agriculture adoption, which is occurring at a slower pace than expected despite the abundant literature on the factors explaining this adoption. This work explores the factors related to agricultural Operations [...] Read more.
Agriculture is undergoing a profound change related to Agriculture 4.0 development and Precision Agriculture adoption, which is occurring at a slower pace than expected despite the abundant literature on the factors explaining this adoption. This work explores the factors related to agricultural Operations Management, farmer behavior, and the farmer mental model, topics little explored in the literature, by applying the Theory of Planned Behavior. Considering the exploratory nature of this work, an exploratory multi-method is applied, consisting of expert interviews, case studies, and modeling. This study’s contributions are a list of factors that can affect this adoption, which complements previous studies, theoretical propositions on the relationships between these factors and this adoption, and a model of irrigation Operations Management built based on these factors and these propositions. This model provides a theoretical framework to study the identified factors, the relationships between them, the theoretical propositions, and the adoption of Precision Agriculture. Furthermore, the results of case studies allow us to explore the relationships between adoption, educational level, and training. The identified factors and the model contribute to broadening the understanding of Precision Agriculture adoption, adding Operations Management and the farmer mental model to previous studies. A future research agenda is formulated to direct future studies. Full article
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<p>Exploratory multi-method.</p>
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<p>Model of irrigation OM (context diagram).</p>
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<p>Model of irrigation Operations Management (IDEF0 diagram).</p>
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19 pages, 5348 KiB  
Article
Pan-Genome-Wide Identification and Transcriptome-Wide Analysis of ZIP Genes in Cucumber
by Zimo Wang, Mengmeng Yin, Jing Han, Xuehua Wang, Jingshu Chang, Zhonghai Ren and Lina Wang
Agriculture 2024, 14(1), 133; https://doi.org/10.3390/agriculture14010133 - 16 Jan 2024
Viewed by 1283
Abstract
The ZRT/IRT-like proteins (ZIPs) play critical roles in the absorption, transport, and intracellular balance of metal ions essential for various physiological processes in plants. However, little is known about the pan-genomic characteristics and properties of ZIP genes in cucumber (Cucumis sativus L.). [...] Read more.
The ZRT/IRT-like proteins (ZIPs) play critical roles in the absorption, transport, and intracellular balance of metal ions essential for various physiological processes in plants. However, little is known about the pan-genomic characteristics and properties of ZIP genes in cucumber (Cucumis sativus L.). In this study, we identified 10 CsZIP genes from the pan-genome of 13 C. sativus accessions. Among them, only CsZIP10 showed no variation in protein sequence length. We analyzed the gene structure, conserved domains, promoter cis-elements, and phylogenetic relationships of these 10 CsZIP genes derived from “9930”. Based on phylogenetic analysis, the CsZIP genes were classified into three branches. Amino acid sequence comparison revealed the presence of conserved histidine residues in the ZIP proteins. Analysis of promoter cis-elements showed that most promoters contained elements responsive to plant hormones. Expression profiling in different tissues showed that most CsZIP genes were expressed at relatively low levels in C. sativus leaves, stems, and tendrils, and CsZIP7 and CsZIP10 were specifically expressed in roots, indicating their potential involvement in the absorption and transport of metal ions. Transcriptomic data indicated that these 10 ZIP genes displayed responses to both downy mildew and powdery mildew, and CsZIP1 was significantly downregulated after both salt and heat treatments. In conclusion, this study deepens our understanding of the ZIP gene family and enhances our knowledge of the biological functions of CsZIP genes in C. sativus. Full article
(This article belongs to the Section Crop Genetics, Genomics and Breeding)
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<p>Phylogenetic tree and gene structure of <span class="html-italic">ZIP</span> family members in <span class="html-italic">C</span>. <span class="html-italic">sativus</span>. The phylogenetic tree was constructed using the neighbor-joining (NJ) method with 1000 bootstrap replicates, based on the alignment of the identified 10 ZIP proteins in <span class="html-italic">C</span>. <span class="html-italic">sativus</span>. The gene structures of the identified 10 <span class="html-italic">ZIP</span> genes in <span class="html-italic">C</span>. <span class="html-italic">sativus</span> were generated utilizing the Gene Structure Display Server v.2.0. In the structures, the green box represents the UTR, the yellow box represents the exon, and the black line represents the intron.</p>
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<p><span class="html-italic">C. sativus</span> ZIP proteins exhibit conserved motifs identified by MEME, highlighted with colored boxes.</p>
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<p>Amino acid sequence alignment for ZIP proteins from <span class="html-italic">Arabidopsis</span>, <span class="html-italic">C</span>. <span class="html-italic">sativus</span>, <span class="html-italic">Cucumis melo</span>, and <span class="html-italic">O. sativa</span>. The alignment of ZIP proteins was conducted via MAFFT v.5.3, employing default settings. Black boxes indicate residues that are entirely conserved, while gray boxes highlight residues with a high level of conservation. * represents the positions of the amino acids, which are 430, 450, and 470, respectively.</p>
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<p>Phylogenetic tree for the ZIP proteins from <span class="html-italic">Arabidopsis</span>, <span class="html-italic">C</span>. <span class="html-italic">sativus</span>, <span class="html-italic">Cucumis melo</span>, and <span class="html-italic">O. sativa</span>. In MEGA 7.0, the neighbor-joining (NJ) method was used to construct a root-free amino acid sequence similarity tree, which was repeated 1000 times.</p>
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<p>Chromosomal location of <span class="html-italic">C</span>. <span class="html-italic">sativus ZIP</span> genes.</p>
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<p>Synteny analysis of <span class="html-italic">ZIPs</span> between <span class="html-italic">C</span>. <span class="html-italic">sativus</span> and other plant species (<span class="html-italic">A. thaliana</span>, <span class="html-italic">Oryza sativa,</span> and <span class="html-italic">Cucumis melo</span>): the collinear blocks are marked by gray lines, while the collinear gene pairs with <span class="html-italic">ZIP</span> genes are highlighted by red lines.</p>
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<p>Synteny analysis of <span class="html-italic">ZIPs</span> between <span class="html-italic">C</span>. <span class="html-italic">sativus</span> and other plant species (<span class="html-italic">A. thaliana</span>, <span class="html-italic">Oryza sativa,</span> and <span class="html-italic">Cucumis melo</span>): the collinear blocks are marked by gray lines, while the collinear gene pairs with <span class="html-italic">ZIP</span> genes are highlighted by red lines.</p>
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<p>Predicted <span class="html-italic">cis</span>-elements in promoter regions of <span class="html-italic">C</span>. <span class="html-italic">sativus ZIP</span> genes. The promoter region was defined as a 1.5 kb sequence upstream of the translation initiation codon of the <span class="html-italic">CsZIP</span> gene. The <span class="html-italic">cis</span>-acting elements were identified utilizing the Plant CARE online tool. Various types of <span class="html-italic">cis</span>-acting elements are denoted by distinctively colored closed boxes.</p>
Full article ">Figure 8
<p>Temporal-spatial expression of <span class="html-italic">C</span>. <span class="html-italic">sativus ZIP</span> genes. (<b>a</b>) The heatmap showcases the expression patterns of <span class="html-italic">CsZIP</span> genes across nine distinct <span class="html-italic">C. sativus</span> tissues. The RNA-seq datasets, acquired via accession number PRJNA80169 from the Cucurbit Genomics Data website, were used. Colors on the scale signify Log<sub>2</sub>(FPKM) values, where blue and red represent low and high expression levels, respectively. Detailed FPKM values for CsZIP genes can be located in <a href="#app1-agriculture-14-00133" class="html-app">Table S1</a>. (<b>b</b>) Validation of RNA-seq results for four <span class="html-italic">CsZIP</span> genes using qRT-PCR. The error bars represent the standard error of the mean (<span class="html-italic">n</span> = 3). FF: female flower; R: root; L: leaf; MF: male flower; O-fer: expanded fertilized ovary; S: stem; O: unexpanded ovary; O-unfer: expanded unfertilized ovary; T: tendril.</p>
Full article ">Figure 9
<p>Expression of <span class="html-italic">CsZIP</span> genes in response to salt and hot stimuli. (<b>a</b>) Heatmap for displaying the expression profile of <span class="html-italic">CsZIP</span> genes in response to salt stresses. (<b>b</b>) The heatmap illustrates the expression patterns of <span class="html-italic">CsZIP</span> genes in response to high temperature (42 °C). RNA-seq datasets with accession numbers GSE151055 and GSE116265 were retrieved from the NCBI SAR database. The color scale is representative of Log<sub>2</sub>(FPKM) values, where green signifies low expression, red represents high expression, and black indicates no expression. The FPKM value of <span class="html-italic">CsZIP</span> genes under salt and hot treatments are listed in <a href="#app1-agriculture-14-00133" class="html-app">Table S2</a>.</p>
Full article ">Figure 10
<p>Expression analysis of <span class="html-italic">CsZIPs</span> under biotic stresses: The transcriptional levels of <span class="html-italic">CsZIP</span> genes after infection with powdery mildew (PM) for 48 h (<b>a</b>) and with downy mildew (DM) for 1–8 days post-inoculation (<b>b</b>) are shown on the heatmaps. The color scale shows increasing expression levels from green to red. ID, PM-inoculated susceptible <span class="html-italic">C</span>. <span class="html-italic">sativus</span> line D8 leaves; NID, non-inoculated D8 leaves; IS, PM-inoculated resistant <span class="html-italic">C</span>. <span class="html-italic">sativus</span> line SSL508-28 leaves; NIS, non-inoculated SSL508-28 leaves; CT, without inoculation; DPI, days post-inoculation. The FPKM value of <span class="html-italic">CsZIP</span> genes under powdery mildew and downy mildew are listed in <a href="#app1-agriculture-14-00133" class="html-app">Table S3</a>.</p>
Full article ">
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