Adipose Autologous Micrograft and Its Derived Mesenchymal Stem Cells in a Bio Cross-Linked Hyaluronic Acid Scaffold for Correction Deep Wrinkles, Facial Depressions, Scars, Face Dermis and Its Regenerations: A Pilot Study and Cases Report
<p>Procedure to mix microfragmented fat with hyaluronic acid. (<b>A</b>) Hyaluronic acid prefilled syringe; (<b>B</b>) Filtration of the collected and fragmented fat tissue; (<b>C</b>,<b>D</b>) mixing of the fragmented adipose tissue with hyaluronic acid in a closed system to guarantee sterility.</p> "> Figure 2
<p>Berardesca Scale for the patient’s satisfaction evaluation. Subjects’ evaluation of their satisfaction in comparison to D0 (before treatment), after 1 day, 80 and 150 days, by giving scores on firmness and cutaneous relief. Scale of 0–4 for each criterion (0 = unsatisfactory; 4 satisfactory).</p> "> Figure 3
<p>Numeric Rating Scale (NRS) evaluating defect severity and wrinkles. 10–0 Numeric Rating Scale (NRS) with separate scores for each site (10 = High wrinkle signs or High defect severity; 5 = Medium wrinkle signs or medium defect severity; 0 = Low wrinkle signs or medium defect severity); * <span class="html-italic">p</span> < 0.05.</p> "> Figure 4
<p>Modified Vancouver scale for the evaluation of ductility, height, vascularity and pigmentation. Modified Vancouver Scale used to estimate the improvement of the skin appearance in comparison to D0 (before treatment), after 1 day, 80 and 150 days. The parameters considered are ductility, height, vascularity and pigmentation. * <span class="html-italic">p</span> > 0.05.</p> "> Figure 5
<p>Example of treatment on a patient. (<b>A</b>) before treatment; (<b>B</b>) 80 days after treatment.</p> ">
Abstract
:1. Introduction
2. Patients and Materials
- Male or female subjects between 30 years and 65 years;
- The presence of deep wrinkles in the facial area, e.g., around the eyes, around the mouth and cheeks, including wrinkles and spots on the face;
- Skin free of diseases that could interfere with the evaluation of the results;
- Willingness to abstain from any cosmetic or surgical procedures in the treatment area for theduration of the clinical investigation;
- Willingness to abstain from any facial surgical procedures for the duration of the clinical;
- investigation including application of botulinum toxin;
- Willingness to abstain from excessive weight gain or weight loss (±10% of body weight), and/or drastic dietary changes for the duration of the clinical investigation;
- Written informed consent.
2.1. Exclusion Criteria
- For females: pregnancy, lactating, planned pregnancy;
- History of mental disorders or emotional instability;
- History of allergic reaction to HA products;
- Facial surgery or implantation of dermal fillers in the nasolabial region within the last 24 months;
- Skin of the to be treated region affected by cosmetic treatments (e.g., laser therapy within the last 12 months, chemical peeling within the last 3 months, and dermabrasion within the last 12 months, and botulinum toxin within the last 12 months);
- Connective tissue diseases;
- Diabetes mellitus or uncontrolled systemic diseases;
- Known human immune deficiency virus-positive individuals;
- Presence of silicone implants or implants of another non-absorbable substance (permanent fillers) in the area of product application;
- Cutaneous lesions in the evaluated area;
- Tendency to keloid formation and/or hypertrophic scars;
- Autoimmune disease;
- Any medical condition prohibiting inclusion in the study according to the judgment of the investigator;
- Subjects for whom due to a mental disorder or a mental disability a custodian has been appointed or who are legally or magisterially arrested or housed;
- Current or previous (within 30 days of enrollment) treatment with an investigational drug and/or medical device or participation in another clinical study;
- History of allergies to cosmetic filling products and recurrent herpes simplex virus;
- Heavy smokers (>20 cigarettes per day).
2.2. Procedure
2.3. Detailed Procedure
2.4. Follow-Ups
3. Results
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
- Mishra, S.K.; Balendra, V.; Esposto, J.; Obaid, A.A.; Maccioni, R.B.; Jha, N.K.; Perry, G.; Moustafa, M.; Al-Shehri, M.; Singh, M.P.; et al. Therapeutic Antiaging Strategies. Biomedicines 2022, 10, 2515. [Google Scholar] [CrossRef]
- De Almeida, A.J.P.O.; de Oliveira, J.C.P.L.; da Silva Pontes, L.V.; de Souza Júnior, J.F.; Gonçalves, T.A.F.; Dantas, S.H.; Feitosa, M.S.D.A.; Silva, A.O.; de Medeiros, I.A. ROS: Basic Concepts, Sources, Cellular Signaling, and its Implications in Aging Pathways. Oxidative Med. Cell. Longev. 2022, 2022, 1225578. [Google Scholar] [CrossRef]
- Leyane, T.S.; Jere, S.W.; Houreld, N.N. Oxidative Stress in Ageing and Chronic Degenerative Pathologies: Molecular Mechanisms Involved in Counteracting Oxidative Stress and Chronic Inflammation. Int. J. Mol. Sci. 2022, 23, 7273. [Google Scholar] [CrossRef]
- Zorina, A.; Zorin, V.; Kudlay, D.; Kopnin, P. Age-Related Changes in the Fibroblastic Differon of the Dermis: Role in Skin Aging. Int. J. Mol. Sci. 2022, 23, 6135. [Google Scholar] [CrossRef]
- Surowiecka, A.; Strużyna, J. Adipose-Derived Stem Cells for Facial Rejuvenation. J. Pers. Med. 2022, 12, 117. [Google Scholar] [CrossRef]
- Martic, I.; Jansen-Dürr, P.; Cavinato, M. Effects of Air Pollution on Cellular Senescence and Skin Aging. Cells 2022, 11, 2220. [Google Scholar] [CrossRef]
- Wang, Y.; Wang, L.; Wen, X.; Hao, D.; Zhang, N.; He, G.; Jiang, X. NF-κB signaling in skin aging. Mech. Ageing Dev. 2019, 184, 111160. [Google Scholar] [CrossRef]
- Kümper, M.; Steinkamp, J.; Zigrino, P. Metalloproteinases in dermal homeostasis. Am. J. Physiol. Physiol. 2022, 323, C1290–C1303. [Google Scholar] [CrossRef]
- Lynch, K.; Pei, M. Age associated communication between cells and matrix: A potential impact on stem cell-based tissue regeneration strategies. Organogenesis 2014, 10, 289–298. [Google Scholar] [CrossRef] [Green Version]
- Sethe, S.; Scutt, A.; Stolzing, A. Aging of mesenchymal stem cells. Ageing Res. Rev. 2006, 5, 91–116. [Google Scholar] [CrossRef]
- Papaccio, F.; Paino, F.; Regad, T.; Papaccio, G.; Desiderio, V.; Tirino, V. Concise Review: Cancer Cells, Cancer Stem Cells, and Mesenchymal Stem Cells: Influence in Cancer Development. Stem Cells Transl. Med. 2017, 6, 2115–2125. [Google Scholar] [CrossRef]
- Kaul, A.; Short, W.D.; Keswani, S.G.; Wang, X. Immunologic Roles of Hyaluronan in Dermal Wound Healing. Biomolecules 2021, 11, 1234. [Google Scholar] [CrossRef]
- Abatangelo, G.; Vindigni, V.; Avruscio, G.; Pandis, L.; Brun, P. Hyaluronic Acid: Redefining Its Role. Cells 2020, 9, 1743. [Google Scholar] [CrossRef]
- Croce, M.; Dyne, K.; Boraldi, F.; Quaglino, D.; Cetta, G.; Tiozzo, R.; Ronchetti, I.P. Hyaluronan affects protein and collagen synthesis by in vitro human skin fibroblasts. Tissue Cell 2001, 33, 326–331. [Google Scholar] [CrossRef]
- Mildmay-White, A. Cell Surface Markers on Adipose-Derived Stem Cells: A Systematic Review. Curr. Stem Cell Res. Ther. 2017, 12, 484–492. [Google Scholar] [CrossRef]
- Bayer, I.S. Hyaluronic Acid and Controlled Release: A Review. Molecules 2020, 25, 2649. [Google Scholar] [CrossRef]
- Aya, K.L.; Stern, R. Hyaluronan in wound healing: Rediscovering a major player. Wound Repair Regen. 2014, 22, 579–593. [Google Scholar] [CrossRef]
- Hemmrich, K.; Van de Sijpe, K.; Rhodes, N.P.; Hunt, J.A.; Di Bartolo, C.; Pallua, N.; Blondeel, P.; von Heimburg, D. Autologous In Vivo Adipose Tissue Engineering in Hyaluronan-Based Gels—A Pilot Study. J. Surg. Res. 2008, 144, 82–88. [Google Scholar] [CrossRef]
- Svolacchia, F.; Svolacchia, L. Adipose tissue micrograft in a scaffold of plasma-gel combined with platelet-derived growth factors in dermal wrinkle regeneration. Scr. Med. 2021, 52, 42–48. [Google Scholar] [CrossRef]
- Svolacchia, F.; Svolacchia, L. Adult Mesenchymal Stem Cells (MSCa) derived from adipose tissue (ADSCa) in a scaffold of free hyaluronic acid in the regeneration of periocular tissues. J. Appl. Cosmetol. 2019, 37. [Google Scholar]
- Rusanov, A.L.; Biryukova, Y.K.; Shoshina, O.O.; Luzgina, E.D.; Luzgina, N.G. Activation of TLR4 of Mesenchymal Stem Cells Enhances the Regenerative Properties of Their Secretomes. Bull. Exp. Biol. Med. 2021, 170, 544–549. [Google Scholar] [CrossRef]
- Luo, L.; Lucas, R.M.; Liu, L.; Stow, L.J. Signalling, sorting and scaffolding adaptors for Toll-like receptors. J. Cell Sci. 2020, 133, 239194. [Google Scholar] [CrossRef]
- Svolacchia, F.; Svolacchia, L. Use of microfiltered vs only disaggregated mesenchymal stem cells from adipose tissue in regenerative medicine. Scr. Med. 2020, 51, 152–157. [Google Scholar] [CrossRef]
- Bi, H.-S.; Zhang, C.; Nie, F.-F.; Pan, B.-L.; Xiao, E. Basic and Clinical Evidence of an Alternative Method to Produce Vivo Nanofat. Chin. Med. J. 2018, 131, 588–593. [Google Scholar] [CrossRef]
- Furno, D.L.; Tamburino, S.; Mannino, G.; Gilia, E.; Lombardo, G.A.G.; Tarico, M.S.; Vancheri, C.; Giuffrida, R.; Perrotta, R.E. Nanofat 2.0: Experimental Evidence for a Fat Grafting Rich in Mesenchymal Stem Cells. Physiol. Res. 2017, 66, 663–671. [Google Scholar] [CrossRef]
- Romieu-Mourez, R.; Francois, M.; Boivin, M.-N.; Bouchentouf, M.; Spaner, D.E.; Galipeau, J. Cytokine Modulation of TLR Expression and Activation in Mesenchymal Stromal Cells Leads to a Proinflammatory Phenotype. J. Immunol. 2009, 182, 7963–7973. [Google Scholar] [CrossRef] [Green Version]
- Tesar, B.M.; Goldstein, D.R. Toll-like receptors and their role in transplantation. Front. Biosci. 2007, 12, 4221–4238. [Google Scholar] [CrossRef] [Green Version]
- DelaRosa, O.; Dalemans, W.; Lombardo, E. Toll-Like Receptors as Modulators of Mesenchymal Stem Cells. Front. Immunol. 2012, 3, 182. [Google Scholar] [CrossRef] [Green Version]
- Zhao, X.; Liu, Y.; Jia, P.; Cheng, H.; Wang, C.; Chen, S.; Huang, H.; Han, Z.; Han, Z.-C.; Marycz, K.; et al. Chitosan hydrogel-loaded MSC-derived extracellular vesicles promote skin rejuvenation by ameliorating the senescence of dermal fibroblasts. Stem Cell Res. Ther. 2021, 12, 1–15. [Google Scholar] [CrossRef]
- Svolacchia, F.; Svolacchia, L. A protocol of a new regenerative treatment of chrono-aging and photo-aging with progenitors cells from adipose Micrograft obtained from MilliGraft® Kit. Acta Sci. Med. Sci. 2019, 3, 30–35. [Google Scholar]
- Tonnard, P.; Verpaele, A.; Peeters, G.; Hamdi, M.; Cornelissen, M.; Declercq, H. Nanofat grafting: Basic research and clinical applications. Plast. Reconstr. Surg. 2013, 132, 1017–1026. [Google Scholar] [CrossRef]
- PROCEDURE: Milligraft n.d. Available online: https://www.milligraft.com/en-procedure/ (accessed on 6 November 2022).
- Berardesca, E.; Distante, F.; Anthoine, P.; Rabbiosi, G.; Aubert, L. Clinical and instrumental evaluation of the activity of an anti-wrinkle cosmetic product on cutaneous relief and photoaged skin. J. Appl. Cosmetol. 1997, 15, 69–75. [Google Scholar]
- Sullivan, T.; Smith, J.; Kermode, J.; Mclver, E.; Courtemanche, D.J. Rating the Burn Scar. J. Burn Care Rehabil. 1990, 11, 256–260. [Google Scholar] [CrossRef]
- Tremolada, C.; Colombo, V.; Ventura, C. Adipose Tissue and Mesenchymal Stem Cells: State of the Art and Lipogems® Technology Development. Curr. Stem Cell Rep. 2016, 2, 304–312. [Google Scholar] [CrossRef] [Green Version]
- Cittadini, E.; Brucculeri, A.M.; Quartararo, F.; Vaglica, R.; Miceli, V.; Conaldi, P.G. Stem cell therapy in the treatment of organic and dysfunctional endometrial pathology. Minerva Obstet. Gynecol. 2021. Online ahead of print. [Google Scholar] [CrossRef]
- Carelli, S.; Messaggio, F.; Canazza, A.; Hebda, D.M.; Caremoli, F.; Latorre, E.; Grimoldi, M.G.; Colli, M.; Bulfamante, G.; Tremolada, C.; et al. Characteristics and Properties of Mesenchymal Stem Cells Derived from Microfragmented Adipose Tissue. Cell Transplant. 2015, 24, 1233–1252. [Google Scholar] [CrossRef] [Green Version]
- Nava, S.; Sordi, V.; Pascucci, L.; Tremolada, C.; Ciusani, E.; Zeira, O.; Cadei, M.; Soldati, G.; Pessina, A.; Parati, E.; et al. Long-Lasting Anti-Inflammatory Activity of Human Microfragmented Adipose Tissue. Stem Cells Int. 2019, 2019, 5901479. [Google Scholar] [CrossRef]
- Han, C.; Weng, X.S.; Cui, Y. Microfragmented adipose tissue and its initial application in articular disease. Chin. Med. J. 2019, 132, 2745–2748. [Google Scholar] [CrossRef]
- Sembronio, S.; Tel, A.; Tremolada, C.; Lazzarotto, A.; Isola, M.; Robiony, M. Temporomandibular Joint Arthrocentesis and Microfragmented Adipose Tissue Injection for the Treatment of Internal Derangement and Osteoarthritis: A Randomized Clinical Trial. J. Oral Maxillofac. Surg. 2021, 79, 1447–1456. [Google Scholar] [CrossRef]
- Laureti, S.; Gionchetti, P.; Cappelli, A.; Vittori, L.; Contedini, F.; Rizzello, F.; Golfieri, R.; Campieri, M.; Poggioli, G. Refractory Complex Crohn’s Perianal Fistulas: A Role for Autologous Microfragmented Adipose Tissue Injection. Inflamm. Bowel Dis. 2020, 26, 321–330. [Google Scholar] [CrossRef]
- Suh, A.; Pham, A.; Cress, M.J.; Pincelli, T.; TerKonda, S.P.; Bruce, A.J.; Zubair, A.C.; Wolfram, J.; Shapiro, S.A. Adipose-derived cellular and cell-derived regenerative therapies in dermatology and aesthetic rejuvenation. Ageing Res. Rev. 2019, 54, 100933. [Google Scholar] [CrossRef]
- Jamari, J.; Ammarullah, M.I.; Santoso, G.; Sugiharto, S.; Supriyono, T.; van der Heide, E. In Silico Contact Pressure of Metal-on-Metal Total Hip Implant with Different Materials Subjected to Gait Loading. Metals 2022, 12, 1241. [Google Scholar] [CrossRef]
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Svolacchia, L.; Prisco, C.; Giuzio, F.; Svolacchia, F. Adipose Autologous Micrograft and Its Derived Mesenchymal Stem Cells in a Bio Cross-Linked Hyaluronic Acid Scaffold for Correction Deep Wrinkles, Facial Depressions, Scars, Face Dermis and Its Regenerations: A Pilot Study and Cases Report. Medicina 2022, 58, 1692. https://doi.org/10.3390/medicina58111692
Svolacchia L, Prisco C, Giuzio F, Svolacchia F. Adipose Autologous Micrograft and Its Derived Mesenchymal Stem Cells in a Bio Cross-Linked Hyaluronic Acid Scaffold for Correction Deep Wrinkles, Facial Depressions, Scars, Face Dermis and Its Regenerations: A Pilot Study and Cases Report. Medicina. 2022; 58(11):1692. https://doi.org/10.3390/medicina58111692
Chicago/Turabian StyleSvolacchia, Lorenzo, Claudia Prisco, Federica Giuzio, and Fabiano Svolacchia. 2022. "Adipose Autologous Micrograft and Its Derived Mesenchymal Stem Cells in a Bio Cross-Linked Hyaluronic Acid Scaffold for Correction Deep Wrinkles, Facial Depressions, Scars, Face Dermis and Its Regenerations: A Pilot Study and Cases Report" Medicina 58, no. 11: 1692. https://doi.org/10.3390/medicina58111692