Design and Prototype of an Automated Column-Switching HPLC System for Radiometabolite Analysis
<p>Flow chart of the processing of a metabolite sample. The plasma sample loaded on to the Sample loop S1 is transferred to the trapping cartridge C1. The materials retained on the trapping cartridge are then separated on the Analytical column C2. All materials from the trapping cartridge and the analytical column are passed through the coincidence detector D1 and UV and/or Mass Spectrometer D2 and collected in the fraction collector F1 for analysis in a well counter.</p> "> Figure 2
<p>Comparison of high performance liquid chromatography (HPLC) chromatogram and plasma fractions counted in a gamma counter for a [<sup>18</sup>F] flurpiridaz sample. (<b>a</b>) Raw chromatogram from inline coincidence detector (60% CH<sub>3</sub>CN in phosphate buffered saline eluted through a C-18 column (Waters Xbridge BEH, 130Å; 100 × 4.6 mm, 3.5 μm) at 1 mL/min; (<b>b</b>) 2 mL fractions from the HPLC analysis were counted on the gamma counter, then plotted as smoothed line for comparison.</p> "> Figure 3
<p>Equilibration of the trapping cartridge and analytical column.</p> "> Figure 4
<p>The loading of the plasma from the sample loop to the trapping cartridge while the analytical column continues to be equilibrated with the eluting solvent.</p> "> Figure 5
<p>The elution of the plasma sample from the trapping cartridge to the analytical column which is analyzed by the coincidence detector, UV detector and/or mass spectrometer.</p> "> Figure 6
<p>If the pressure of the analytical column exceeds the set pressure limit, the 10-port column direction valve V3 is switched, the analytical column flow is reversed to remove materials obstructing the flow of solvent. Once the pressure drops the valve is switched and the flow returned to normal flow, as per Step 3.</p> ">
Abstract
:1. Introduction
2. Results and Discussion
3. Experimental Section
Acknowledgments
Author Contributions
Conflicts of Interest
Abbreviations
CNS | Central nervous system |
PET | Positron emission tomography |
HPLC | High performance liquid chromatography |
UV | Ultraviolet |
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Vasdev, N.; Collier, T.L. Design and Prototype of an Automated Column-Switching HPLC System for Radiometabolite Analysis. Pharmaceuticals 2016, 9, 51. https://doi.org/10.3390/ph9030051
Vasdev N, Collier TL. Design and Prototype of an Automated Column-Switching HPLC System for Radiometabolite Analysis. Pharmaceuticals. 2016; 9(3):51. https://doi.org/10.3390/ph9030051
Chicago/Turabian StyleVasdev, Neil, and Thomas Lee Collier. 2016. "Design and Prototype of an Automated Column-Switching HPLC System for Radiometabolite Analysis" Pharmaceuticals 9, no. 3: 51. https://doi.org/10.3390/ph9030051