Immunopotentiating Effect of an Ayurvedic Preparation from Medicinal Plants

RESEARCH LETTERS

Dnyaneshwar Umrao Bawankule, Dayanandan Mani, Anirban Pal, Karuna Shanker, Narayan Prasad Yadav, Sachidanand Yadav, Amit Kumar Srivastava, Jyoti Agarwal, Ajit Kumar Shasany, Mahendra Pandurang Darokar, Madan Mohan Gupta, Suman Preet Singh Khanuja

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Dnyaneshwar Umrao Bawankule
Genetic Resources and Biotechnology Division
Dayanandan Mani
Genetic Resources and Biotechnology Division
Anirban Pal
Genetic Resources and Biotechnology Division
Karuna Shanker
Analytical Chemistry Divisions, Central Institute of Medicinal and Aromatic Plants (CIMAP), Council of Scientific and Industrial Research (CSIR)
Narayan Prasad Yadav
Genetic Resources and Biotechnology Division
Sachidanand Yadav
Genetic Resources and Biotechnology Division
Amit Kumar Srivastava
Genetic Resources and Biotechnology Division
Jyoti Agarwal
Genetic Resources and Biotechnology Division
Ajit Kumar Shasany
Genetic Resources and Biotechnology Division
Mahendra Pandurang Darokar
Genetic Resources and Biotechnology Division
Madan Mohan Gupta
Analytical Chemistry Divisions, Central Institute of Medicinal and Aromatic Plants (CIMAP), Council of Scientific and Industrial Research (CSIR)
Suman Preet Singh Khanuja
Genetic Resources and Biotechnology Division

Keywords: CIM-Candy, Ocimum sanctum, Phyllanthus emblica, Glycyrrhiza glabra, immunopotentiation, humoral response

JOURNAL FREE ACCESS

2009 Volume 55 Issue 2 Pages 285-289

DOI https://doi.org/10.1248/jhs.55.285

Details

Abstract

A formulation of Ocimum sanctum, Phyllanthus emblica, and Glycyrrhiza glabra in a defined ratio termed CIM-Candy was prepared based on Ayurvedic methodology. To study the humoral immune response, rabbit red blood corpuscles (rRBCs) were used as an antigen. Mice were immunized on day 7 and 14 days the start of the experiment using an intraperitoneal injection of 200 μl of rRBCs (2×10⁸ cells/ml). Humoral immune response, body weight variation, and hematology parameters were studied. CIM-Candy at doses of 10 and 100 mg/kg body weight enhanced the immune function by significantly increasing (p<0.05) the hemagglutination antibody titer response against the rRBCs. Significant changes were not observed in body weight and hematology parameters when comparing the normal vehicle control and treatment groups. As the biological activity of the plant-derived formulation was not assigned to a single chemical, high performance thin layer chromatography (HPTLC) fingerprinting was also performed to confirm the chemical characteristic of the formulation. The results of this study suggest that the standardized formulation CIM-Candy exhibited significant humoral immune response in Swiss albino mice.

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