Composite map of the C. elegans Intron Lariat Spliceosome primed for disassembly (ILS')
Resolution: 2.9 Å
EM Method: Single-particle
Fitted PDBs: 8ro0
Q-score: 0.372
Vorlander MK, Rothe P, Kleifeld J, Cormack ED, Veleti L, Riabov-Bassat D, Fin L, Phillips AW, Cochella L, Plaschka C
Nature (2024) 632 pp. 443-450 [ DOI: doi:10.1038/s41586-024-07741-1 Pubmed: 38925148 ]
- Pre-mrna-splicing factor syf-2 (27 kDa, Protein from Caenorhabditis elegans)
- Intron-binding protein aquarius (170 kDa, Protein from Caenorhabditis elegans)
- Pre-mrna-splicing factor syf1 (99 kDa, Protein from Caenorhabditis elegans)
- Peptidyl-prolyl cis-trans isomerase (18 kDa, Protein from Caenorhabditis elegans)
- Peptidyl-prolyl cis-trans isomerase e (36 kDa, Protein from Caenorhabditis elegans)
- Tpr_region domain-containing protein (88 kDa, Protein from Caenorhabditis elegans)
- Protein isy-1 (31 kDa, Protein from Caenorhabditis elegans)
- Magnesium ion (24 Da, Ligand)
- Probable small nuclear ribonucleoprotein sm d2 (13 kDa, Protein from Caenorhabditis elegans)
- U2 snrna (56 kDa, RNA from Caenorhabditis elegans)
- Probable small nuclear ribonucleoprotein g (8 kDa, Protein from Caenorhabditis elegans)
- Intron lariat rna (3 kDa, DNA from Caenorhabditis elegans)
- Small nuclear ribonucleoprotein sm d1 (13 kDa, Protein from Caenorhabditis elegans)
- Probable small nuclear ribonucleoprotein-associated protein b (16 kDa, Protein from Caenorhabditis elegans)
- Gcf c-terminal domain-containing protein (94 kDa, Protein from Caenorhabditis elegans)
- Uncharacterized protein t27f2.1 (60 kDa, Protein from Caenorhabditis elegans)
- Inositol hexakisphosphate (660 Da, Ligand)
- Wd_repeats_region domain-containing protein (65 kDa, Protein from Caenorhabditis elegans)
- Probable u2 small nuclear ribonucleoprotein a' (28 kDa, Protein from Caenorhabditis elegans)
- U5 snrna (38 kDa, RNA from Caenorhabditis elegans)
- Wd_repeats_region domain-containing protein (36 kDa, Protein from Caenorhabditis elegans)
- U5 small nuclear ribonucleoprotein 200 kda helicase (244 kDa, Protein from Caenorhabditis elegans)
- U6 snrna (32 kDa, RNA from Caenorhabditis elegans)
- Pre-mrna-splicing factor spf27 (27 kDa, Protein from Caenorhabditis elegans)
- Rrm domain-containing protein (24 kDa, Protein from Caenorhabditis elegans)
- Coiled-coil domain-containing protein 12 (19 kDa, Protein from Caenorhabditis elegans)
- Probable small nuclear ribonucleoprotein e (10 kDa, Protein from Caenorhabditis elegans)
- Small nuclear ribonucleoprotein sm d3 (14 kDa, Protein from Caenorhabditis elegans)
- Tr-type g domain-containing protein (110 kDa, Protein from Caenorhabditis elegans)
- Wd_repeats_region domain-containing protein (54 kDa, Protein from Caenorhabditis elegans)
- Zinc ion (65 Da, Ligand)
- Cell division cycle 5-like protein (85 kDa, Protein from Caenorhabditis elegans)
- Intron lariat spliceosome (Complex from Caenorhabditis elegans)
- Septin and tuftelin-interacting protein 1 homolog (94 kDa, Protein from Caenorhabditis elegans)
- Pre-mrna-processing factor 19 (53 kDa, Protein from Caenorhabditis elegans)
- Pre-mrna-splicing factor 8 homolog (272 kDa, Protein from Caenorhabditis elegans)
- Spliceosome-associated protein cwc15 homolog (26 kDa, Protein from Caenorhabditis elegans)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Pre-mrna-splicing factor rbm22 (45 kDa, Protein from Caenorhabditis elegans)
- Probable small nuclear ribonucleoprotein f (9 kDa, Protein from Caenorhabditis elegans)
- Protein bud31 homolog (17 kDa, Protein from Caenorhabditis elegans)
Structure of the C. elegans Intron Lariat Spliceosome double-primed for disassembly (ILS'')
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 8ro1
Q-score: 0.373
Vorlander MK, Rothe P, Kleifeld J, Cormack ED, Veleti L, Riabov-Bassat D, Fin L, Phillips AW, Cochella L, Plaschka C
Nature (2024) 632 pp. 443-450 [ DOI: doi:10.1038/s41586-024-07741-1 Pubmed: 38925148 ]
- Pre-mrna-splicing factor syf-2 (27 kDa, Protein from Caenorhabditis elegans)
- Intron-binding protein aquarius (170 kDa, Protein from Caenorhabditis elegans)
- Pre-mrna-splicing factor syf1 (99 kDa, Protein from Caenorhabditis elegans)
- Peptidyl-prolyl cis-trans isomerase (18 kDa, Protein from Caenorhabditis elegans)
- Tpr_region domain-containing protein (88 kDa, Protein from Caenorhabditis elegans)
- Protein isy-1 (31 kDa, Protein from Caenorhabditis elegans)
- U2 snrna (68 kDa, RNA from Caenorhabditis elegans)
- Magnesium ion (24 Da, Ligand)
- Probable small nuclear ribonucleoprotein sm d2 (13 kDa, Protein from Caenorhabditis elegans)
- Cwf19-like protein 1 homolog (59 kDa, Protein from Caenorhabditis elegans)
- Coiled-coil domain-containing protein 12 (8 kDa, Protein from Caenorhabditis elegans)
- Probable small nuclear ribonucleoprotein f (9 kDa, Protein from Caenorhabditis elegans)
- Probable small nuclear ribonucleoprotein g (8 kDa, Protein from Caenorhabditis elegans)
- Small nuclear ribonucleoprotein sm d1 (13 kDa, Protein from Caenorhabditis elegans)
- Probable small nuclear ribonucleoprotein-associated protein b (16 kDa, Protein from Caenorhabditis elegans)
- Gcf c-terminal domain-containing protein (94 kDa, Protein from Caenorhabditis elegans)
- Uncharacterized protein t27f2.1 (60 kDa, Protein from Caenorhabditis elegans)
- Pre-mrna-splicing factor atp-dependent rna helicase ddx-15 (84 kDa, Protein from Caenorhabditis elegans)
- Wd_repeats_region domain-containing protein (65 kDa, Protein from Caenorhabditis elegans)
- Inositol hexakisphosphate (660 Da, Ligand)
- Probable u2 small nuclear ribonucleoprotein a' (28 kDa, Protein from Caenorhabditis elegans)
- U5 snrna (35 kDa, RNA from Caenorhabditis elegans)
- Peptidyl-prolyl cis-trans isomerase e (8 kDa, Protein from Caenorhabditis elegans)
- Intron lariat rna (10 kDa, RNA from Caenorhabditis elegans)
- Wd_repeats_region domain-containing protein (36 kDa, Protein from Caenorhabditis elegans)
- Pre-mrna-splicing factor spf27 (27 kDa, Protein from Caenorhabditis elegans)
- U6 snrna (32 kDa, RNA from Caenorhabditis elegans)
- Rrm domain-containing protein (24 kDa, Protein from Caenorhabditis elegans)
- Probable small nuclear ribonucleoprotein e (10 kDa, Protein from Caenorhabditis elegans)
- Small nuclear ribonucleoprotein sm d3 (14 kDa, Protein from Caenorhabditis elegans)
- Tr-type g domain-containing protein (110 kDa, Protein from Caenorhabditis elegans)
- Wd_repeats_region domain-containing protein (54 kDa, Protein from Caenorhabditis elegans)
- Zinc ion (65 Da, Ligand)
- Cell division cycle 5-like protein (85 kDa, Protein from Caenorhabditis elegans)
- Septin and tuftelin-interacting protein 1 homolog (94 kDa, Protein from Caenorhabditis elegans)
- Replication stress response regulator sde2 (57 kDa, Protein from Caenorhabditis elegans)
- Intron lariat spliceosome (ils'') (Complex from Caenorhabditis elegans)
- Pre-mrna-processing factor 19 (53 kDa, Protein from Caenorhabditis elegans)
- Pre-mrna-splicing factor 8 homolog (272 kDa, Protein from Caenorhabditis elegans)
- Spliceosome-associated protein cwc15 homolog (26 kDa, Protein from Caenorhabditis elegans)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Pre-mrna-splicing factor rbm22 (45 kDa, Protein from Caenorhabditis elegans)
- Cwf19-like protein 2 homolog (53 kDa, Protein from Caenorhabditis elegans)
- Protein bud31 homolog (17 kDa, Protein from Caenorhabditis elegans)
Structure of the C. elegans Intron Lariat Spliceosome (Map 1)
Vorlander MK, Rothe P, Kleifeld J, Cormack ED, Veleti L, Riabov-Bassat D, Fin L, Phillips AW, Cochella L, Plaschka C
Nature (2024) 632 pp. 443-450 [ DOI: doi:10.1038/s41586-024-07741-1 Pubmed: 38925148 ]
Structure of the C. elegans Intron Lariat Spliceosome (Map 2)
Vorlander MK, Rothe P, Kleifeld J, Cormack ED, Veleti L, Riabov-Bassat D, Fin L, Phillips AW, Cochella L, Plaschka C
Nature (2024) 632 pp. 443-450 [ DOI: doi:10.1038/s41586-024-07741-1 Pubmed: 38925148 ]
Structure of the C. elegans Intron Lariat Spliceosome (Map 3)
Vorlander MK, Rothe P, Kleifeld J, Cormack ED, Veleti L, Riabov-Bassat D, Fin L, Phillips AW, Cochella L, Plaschka C
Nature (2024) 632 pp. 443-450 [ DOI: doi:10.1038/s41586-024-07741-1 Pubmed: 38925148 ]
Structure of the C. elegans Intron Lariat Spliceosome (Map 4)
Vorlander MK, Rothe P, Kleifeld J, Cormack ED, Veleti L, Riabov-Bassat D, Fin L, Phillips AW, Cochella L, Plaschka C
Nature (2024) 632 pp. 443-450 [ DOI: doi:10.1038/s41586-024-07741-1 Pubmed: 38925148 ]
Structure of the C. elegans Intron Lariat Spliceosome (Map 5)
Vorlander MK, Rothe P, Kleifeld J, Cormack ED, Veleti L, Riabov-Bassat D, Fin L, Phillips AW, Cochella L, Plaschka C
Nature (2024) 632 pp. 443-450 [ DOI: doi:10.1038/s41586-024-07741-1 Pubmed: 38925148 ]
Structure of the C. elegans Intron Lariat Spliceosome (Map 6)
Vorlander MK, Rothe P, Kleifeld J, Cormack ED, Veleti L, Riabov-Bassat D, Fin L, Phillips AW, Cochella L, Plaschka C
Nature (2024) 632 pp. 443-450 [ DOI: doi:10.1038/s41586-024-07741-1 Pubmed: 38925148 ]
Structure of the C. elegans Intron Lariat Spliceosome (Map 7)
Vorlander MK, Rothe P, Kleifeld J, Cormack ED, Veleti L, Riabov-Bassat D, Fin L, Phillips AW, Cochella L, Plaschka C
Nature (2024) 632 pp. 443-450 [ DOI: doi:10.1038/s41586-024-07741-1 Pubmed: 38925148 ]
Structure of the C. elegans Intron Lariat Spliceosome (Map 8)
Vorlander MK, Rothe P, Kleifeld J, Cormack ED, Veleti L, Riabov-Bassat D, Fin L, Phillips AW, Cochella L, Plaschka C
Nature (2024) 632 pp. 443-450 [ DOI: doi:10.1038/s41586-024-07741-1 Pubmed: 38925148 ]