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2021, The 1st International Electronic Conference on Biological Diversity, Ecology and Evolution
2021 •
Technological and analytical advances to study evolutionary biology, ecology, and conservation of green turtles (Chelonia mydas) are realized through molecular approaches including DNA barcoding. We characterized the usefulness of COI DNA barcodes in green turtles in Mexico to better understand genetic divergence and other genetic parameters of this species. We analyzed 63 sequences, including 25 from green turtle field specimens collected from the Gulf of Mexico and from the Mexican Pacific and 38 already present in the Barcode of Life Data Systems (BOLD). A total of 13 haplotypes were identified with four novel haplotypes from the Pacific Ocean and three novel haplotypes from the Atlantic Ocean. Intraspecific distance values among COI gene sequences by two different models were 0.01, demonstrating that there is not a subdivision for green turtle species. Otherwise, the interspecific distance interval ranged from 0.07 to 0.13, supporting a clear subdivision among all sea turtle species. Haplotype and total nucleotide diversity values of the COI gene reflect a medium genetic diversity average. Green turtles of the Mexican Pacific showed common haplotypes to some Australian and Chinese turtles, but different from the haplotypes of the Mexican Atlantic. COI analysis revealed new haplotypes and confirmed that DNA barcodes were useful for evaluation of the population diversity of green turtles in Mexico.
2016 •
1997 •
The Kemp's ridley sea turtle (Lepidochelys kempi) is restricted to the warm temperate zone of the North Atlantic Ocean, whereas the olive ridley turtle (L. olivacea) is globally distributed in warm-temperate and tropical seas, including nesting colonies in the North Atlantic that nearly overlap the range of L. kempi. To explain this lopsided distribution, Pritchard (1969) proposed a scenario in which an ancestral taxon was divided into Atlantic and Pacific forms (L. kempi and L. olivacea, respectively) by the Central American land bridge. According to this model, the olive ridley subsequently occupied the Pacific and Indian Oceans and recently colonized the Atlantic Ocean via southern Africa. To assess this biogeographic model, a 470 bp sequence of the mtDNA control region was compared among 89 ridley turtles, including the sole L. kempi nesting population and 7 nesting locations across the range of L. olivacea. These data confirm a fundamental partition between L. olivacea and L. kempi (p=0.052-0.069), shallow separations within L. olivacea (p=0.002-0.031), and strong geographic partitioning of mtDNA lineages. The most divergent L. olivacea haplotype is observed in the Indo-West Pacific region, as are the central haplotypes in a parsimony network, implicating this region as the source of the most recent radiation of olive ridley lineages. The most common olive ridley haplotype in Atlantic samples is distinguished from an Indo-West Pacific haplotype by a single nucleotide substitution, and East Pacific samples are distingushed from the same haplotype by two nucleotide substitutions. These shallow separations are consistent with the recent invasion of the Atlantic postulated by Pritchard (1969), and indicate that the East Pacific nesting colonies were also recently colonized from the Indo-West Pacific region. Molecular clock estimates place these invasions within the last 300,000 years.
IOP Conference Series: Earth and Environmental Science
DNA barcoding of sea turtles (Dermochelyidae and Cheloniidae) and its protocol using different tissues quality: implication to conservation managers2019 •
Conservation effort of sea turtles faces several challenges, e.g. habitat destruction, eggs and turtle meat consumptions, or production of souvenirs (e.g. turtle bodies). In their duties, conservation officers may find sea turtles in different condition (e.g. body fragments, eggs, pieces of meat, etc.), usually it is difficult to identify due to incomplete morphology. Therefore, the use of DNA barcoding becomes an alternative for for identification at species level, contribute taxonomic and biodiversity research. This study was conducted to develop a protocol suitable for identifying sea turtles from different tissue samples conditions using DNA barcoding. A total of 16 tissue samples in different condition (fresh, dead-body, smoked-meat) were collected. A protocol was developed to enable identification of tissue samples of different quality or condition. A 719 bp control region fragment was analyzed. The high percentage similarity was confirmed in GenBank CO1 sequence with 99%-100%...
Divus Thomas 127 (2024) 1, pp. 51-78
Conoscenza e insegnamento umano all'interno della provvidenza divina: una prospettiva tomistaEPHEMERIS DACOROMANA. Serie nuova
FROM BOSIE TO BOSSY. CONTRIBUTION TO THE BIOGRAPHY OF A ROMANIAN DIPLOMAT IN ITALY2020 •
La Vall de Verç núms 489 i 490
Aquella talla gòtica que els santjustencs vàrem deixar perdre2023 •
Annals of Tropical Medicine and Public Health
Histopathological Findings in Endometrial Biopsies for a Sample of Iraqi Women with Abnormal Uterine Bleeding2020 •
2002 •
Vìsnik nauki ta osvìti
ПЕДАГОГІКА, ЗОРІЄНТОВАНА НА ІННОВАЦІЇ: ОСОБИСТІСНИЙ ВИМІР2024 •
2020 •
A. Alonso Aguirre