Additional file 1 of Heterologous redox partners supporting the efficient catalysis of epothilone B biosynthesis by EpoK in Schlegelella brevitalea

Additional file 1: Figure S1. Alignment of the tertiary structures of P450cam and EpoK. Figure S2. Visual representation of the location of the six selected ferredoxin genes and two selected ferredoxin reductase genes. Figure S3. Multiple sequence alignment of EpoK from three sources. Figure S4. Results of colony PCR to verify the transformation of plasmids pEOK102 to pEOK116 into H7029-1. Table S1. Yields and component proportion of epothilones in mutants of strain H7029-1. Figure S5. Comparison of the protein sequence and structural alignment between Fdx_0135 and Fdx_A6445. Figure S6. HPLC results of epothilone C crude extracts. Figure S7. Results of colony PCR to verify the transformation of the corresponding plasmids into the wild-type strain DSM 7029. Figure S8. Verification of fdx_0135 knock-out by two-step homologous recombination in strain H7029-1. Figure S9. Comparison of the loss rate of plasmid pEOK114 (oriV-trfA-Aprar-Pkan-epoK-Pkan-fdx_0135-Pkan-fdr_0130) in strains JH0...