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OPEN
Received: 27 May 2016
Accepted: 25 October 2016
Published: 24 November 2016
Measles virus induces persistent
infection by autoregulation of viral
replication
Tomomitsu Doi, Hyun-Jeong Kwon, Tomoyuki Honda, Hiroki Sato, Misako Yoneda &
Chieko Kai
Natural infection with measles virus (MV) establishes lifelong immunity. Persistent infection with MV
is likely involved in this phenomenon, as non-replicating protein antigens never induce such longterm immunity. Although MV establishes stable persistent infection in vitro and possibly in vivo, the
mechanism by which this occurs is largely unknown. Here, we demonstrate that MV changes the
infection mode from lytic to non-lytic and evades the innate immune response to establish persistent
infection without viral genome mutation. We found that, in the persistent phase, the viral RNA level
declined with the termination of interferon production and cell death. Our analysis of viral protein
dynamics shows that during the establishment of persistent infection, the nucleoprotein level
was sustained while the phosphoprotein and large protein levels declined. The ectopic expression
of nucleoprotein suppressed viral replication, indicating that viral replication is self-regulated by
nucleoprotein accumulation during persistent infection. The persistently infected cells were able to
produce interferon in response to poly I:C stimulation, suggesting that MV does not interfere with
host interferon responses in persistent infection. Our results may provide mechanistic insight into the
persistent infection of this cytopathic RNA virus that induces lifelong immunity.
Measles is a highly contagious human disease caused by acute infection with measles virus (MV) and remains an
important cause of child morbidity and mortality worldwide1. However, unlike most other viral diseases, infected
individuals acquire life-long protective immunity against MV after recovery2,3. Although a full explanation for
life-long immunity against MV has remained elusive, the 1846 island studies conducted by Panum in the Faroes
and the more recent work conducted in the United States2, which both demonstrated life-long immunity after MV
infection in the absence of reinfection, suggest the involvement of persistent MV infection in this phenomenon.
MV is a non-segmented, negative-sense, single-stranded RNA virus of the genus Morbillivirus within the family Paramyxoviridae, order Mononegavirales4. MV has six structural proteins and two accessory proteins encoded
in a 16-kb RNA genome. The viral genome and replicated positive-sense viral RNA (vRNA) are encapsidated with
nucleoprotein (N), making them a target of viral replication. A polymerase complex composed of phosphoprotein (P) and large protein (L) transcribes both viral mRNA and the viral genome from the encapsidated vRNA.
The hemagglutinin (H) and fusion (F) proteins are involved in attachment and entry into target cells and also in
syncytium formation and cytolysis. Matrix protein (M) is involved in virion budding from the infected cell. The
two accessory proteins, V and C, are involved in interference with host immunity5.
MV is highly cytopathic and commonly causes acute severe infection, resulting in measles and, for one out
of 1,000 measles cases, acute demyelinating encephalitis. However, MV also causes subacute sclerosing panencephalitis (SSPE) at a rate of one out of 1,700–3,300 measles cases if the subject is younger than five years of age
when he/she is infected with MV6. Measles inclusion body encephalitis (MIBE) is an additional central nervous
system infection that occurs in immunocompromised patients7. Thus, MV has the potential to cause persistent
infection, although the occurrence of these diseases is very low and MV persistence has only been found in the
brain in these cases. It seems likely that these diseases are induced after the long-term persistence of MV in the
central nervous system; however, it cannot be excluded that MV persists elsewhere in the body. Furthermore,
the persistent infection with MV in SSPE has been assumed to be a result of the appearance of mutant viruses,
Laboratory Animal Research Center and International Research Center for Infectious Diseases, Institute of Medical
Science, The University of Tokyo, Tokyo, Japan. Correspondence and requests for materials should be addressed to
C.K. (email: ckai@ims.u-tokyo.ac.jp)
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rather than being a natural property of wildtype strains, because many mutations have been found in the MV
genomes isolated from SSPE patients8–11.
MV is known to establish persistent infection in culture12–14. The persistently infected cells show characteristic
features, such as a disappearance of the cytopathic effect, a marked reduction in cell-free virion production, and
a resistance to superinfection by closely related viruses. Although common mutations responsible for persistent
infection have not been determined, temperature-sensitive mutants and defective interfering (DI) particles were
suggested to play a role in persistent infection through restricting viral replication15–19. Interferon (IFN) was also
suggested to be involved in persistent infection with MV, through restricting viral replication20. Receptor downregulation21 and receptor redistribution22 may account for the disappearance of syncytium formation and the
resistance to superinfection with the parent virus, but this downregulation or redistribution does not account for
the resistance to superinfection with closely related canine distemper virus, which does not use the same receptor,
and the reduction of cell-free virion release13. Despite the proposition of several possibilities, however, the mechanisms responsible for MV persistent infection are still under debate.
Griffin and colleagues provided the first evidence for more reproducible persistent MV infection with MV
lacking viral mutations in human and primates23–27. Additionally, MV strains isolated from the brain that are very
similar to the epidemic MV strain were also reported in MIBE patients, probably due to the early onset of MIBE
after a short period of persistence in immunocompromised patients28 These reports suggest that persistent infection is a natural property of wildtype MV. In this study, we show that MV establishes persistent infection without
mutations in culture, and we unveil one potential mechanism of persistent infection by this highly cytopathic
RNA virus.
Results
MV establishes a persistent infection in culture. To determine whether or not mutations in the viral
genome are dispensable for the establishment of persistent MV infection, we analysed cells and viruses during the
early phase of persistent MV infection in cultured cells. According to previous studies, MV establishes persistent
infection in virtually any cell type12–14. Thus, we used B95a cells, which are marmoset lymphoblastoid cells, to
investigate the mechanism of persistent infection because these cells are highly susceptible to MV infection and
can easily achieve 100% infection during persistent infection. In the early phase of MV infection (4 days post-infection [dpi]), most of the cells formed syncytia and died (Fig. 1a). The few remaining cells, however, started
to grow again (Fig. 1a). To determine when all of the cells in culture had established a persistent infection, we
monitored cellular growth and cell death during the course of MV infection (Fig. 1b and c). Although syncytium
formation and cell death faded out at 2 weeks post-infection (Fig. 1a and c), cell growth was severely impaired
until 28 dpi when cells started to grow in a manner similar to that of uninfected controls (Fig. 1b). A second crisis
occurred between 23 and 28 dpi in all three cultures independently, prior to the re-establishment of cell growth
(Fig. 1c). After the establishment of persistent infection, the cell morphology was indistinguishable from that of
uninfected cells (Fig. 1a).
Staining of intracellular MV N protein confirmed that all of the cells in culture were infected with MV (Fig. 1d).
Because a reduction in the amount of infectious virion release has been reported in MV persistent infection13,
we determined the titre of released infectious virions in the persistently infected B95a cells by inoculating the
supernatant onto fresh B95a cells. Although a syncytium-forming virus was released from the persistently
infected B95a cells, the virus titre was two orders of magnitude lower than that obtained during acute infection
(Fig. 1e). It was necessary to confirm that all of the cells in this culture were infected with MV to distinguish true
persistent infection from ‘carrier culture,’ which represents a dynamic equilibrium between infected cell death
and new infection of uninfected cells in culture22.
To more sensitively determine when during the establishment of persistent infection all of the cells had
been infected, we used rMV-EGFP, a recombinant measles virus expressing enhanced green fluorescent protein
(EGFP), because it allowed the direct visualisation of infected cells. Like conventional rMV, rMV-EGFP also
induced a second crisis followed by the establishment of persistent infection with the same kinetics as those of
conventional rMV. The majority of cells were infected with rMV-EGFP at 4 dpi, though GFP expression at 4 dpi
was lower than that at 35 dpi (Fig. 1f). Until 28 dpi, we observed three populations with distinct GFP expression
(GFPnegative, GFPlow, and GFPhigh). The expression level of GFP in GFPlow and GFPhigh corresponded to that of 4 dpi
and 35 dpi, respectively. GFPnegative cells (uninfected cells) were observed until 28 dpi, and, notably, the number
of uninfected cells was increased at 21 dpi, which coincided with the time of the second crisis (Fig. 1c). Thus, the
continuous infection of uninfected cells continued until 28 dpi and was particularly prevalent during the second
crisis.
Virus mutation is dispensable for persistent infection. In previous studies, it was not clear whether
mutations found in the viral genome were the cause or the result of long-term persistent infection because the
mutations were analysed after SSPE onset or after a long period of in vitro culture. To address this question, we
investigated if the appearance of mutant virus was involved in the establishment of persistent MV infection.
First, we determined if functional H and F proteins were expressed on the surface of persistently infected B95a
cells by co-culturing them with uninfected B95a cells, as it has been reported that the fusogenic activity of MV is
related to its cytotoxicity29. Persistently infected B95a cells formed syncytia with uninfected B95a cells within 2 h
(Fig. 2a). This finding indicates that persistently infected B95a cells express functional H and F proteins on the
surface and that they cause cell-to-cell fusion via the virus receptor, signalling lymphocyte activation molecule
(SLAM), which is highly expressed on the cell surface of uninfected B95a cells.
DI particles were shown to be involved in persistent infection with MV19. To further examine the involvement
of DI particles in MV persistence, we aimed to assess 5′ copy-back DI particle genomes by reverse-transcription
polymerase chain reaction (RT-PCR). Although it is difficult to prove the absence of DI particles, DI particles
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Figure 1. Establishment of cell lines with persistent MV infection. B95a cells were infected with rMV in
(a–e). (a) Cellular images of B95a cells, uninfected or at 4 days, 2 weeks, or 2 months post-MV infection.
(b) Growth curve of B95a cells during the establishment phase of MV persistent infection. MV-infected B95a
cells were counted every 5 days from 17 dpi. Control uninfected cells were also counted from same day. Error
bars are SD of three independent infections. (c) Cell death of MV-infected B95a cells. Genomic DNA content
was analysed by propidium iodide staining. The percentages of sub-G1 populations are graphed as dead cells.
(d) Intracellular MV N protein staining of persistently MV-infected B95a cells. Filled and open histograms
indicate uninfected and persistently MV-infected B95a cells, respectively. (e) Cell-free virion release from
acute and persistent MV infection. For acute infection, B95a cells were infected with rMV-HL for 1 h at a MOI
of 0.1 followed by culture medium exchange. Concurrently, equal numbers of persistently rMV-infected B95a
cells were cultured, and 3 days later, the titres of three independent acute infections and three independently
established persistently MV-infected cells were determined and graphed. Horizontal bars indicate averages. The
cellular image shows the level of syncytia formation induced by virus released from persistently MV-infected
cells. (f) B95a cells were infected with rMV-EGFP as described above. EGFP fluorescence was analysed by flow
cytometry at the indicated times post-infection. Markers indicate the range of the EGFP-negative population.
were not observed either in acute or persistent MV infection in our system (Fig. 2b). The relative expression of
MV genes in persistently infected cells showed a gradient of expression like that typically seen in viruses of the
order Mononegavirales (Fig. S1)30. These results indicate that aberrant gene expression and aberrant genomic
fragment accumulation are not involved in persistent MV infection.
Finally, we determined the consensus genomic sequence of the persistent virus. The whole viral genome
sequence was amplified by PCR as fragments and sequenced directly (data not shown). In agreement with our
other results, this analysis did not reveal any mutations in persistent MV.
Down-modulation of surface SLAM prevents syncytium formation.
Because persistent MV replicated well in culture in the absence of viral mutations, without causing cell death, and successfully released
infectious virus into the supernatant (Fig. 1e), we investigated if the cellular features of cells persistently infected
with MV were altered. We examined the surface expression of the MV receptor SLAM in infected cells during the
course of persistent MV infection. The surface expression of SLAM was gradually down-modulated beginning at
4 dpi, and it completely disappeared by 3 weeks after infection (Fig. 2c). Intracellular staining of SLAM showed
that SLAM protein was expressed in the cytoplasm in persistently infected cells to a similar extent as in the parental B95a cells (Fig. 2d), indicating that the expression of this molecule at the cell surface was suppressed.
vRNA is kept at low levels in the persistent phase of MV infection.
To investigate the mechanism
responsible for the reduction in infectious virion release during the persistent phase of MV infection, MV RNA
levels were monitored during the establishment of persistent infection. The gene expressions of IFN-β and IFN
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Figure 2. Syncytium formation when surface SLAM is downregulated. (a) From left to right: B95a cells, cocultured B95a and B95a-MV cells, and B95a-MV cells. (b) Schematic of the MV genome (upper) and 5′ copy
back DI genome (lower). Arrows indicate primers used for detection of the DI genome. The gel image shows
the results of RT-PCR for the MV genome and DI genome, with acutely and persistently MV-infected B95a cells
indicated by “a” and “p”, respectively. The expected length of the PCR products of primer pairs AB and AC are
206 and 266 bp, respectively. The sizes of the molecular size marker bands near the PCR products are indicated.
(c) SLAM expression on the surface of B95a cells at the indicated time-points after MV infection. Filled and
open histograms indicate uninfected and persistently MV-infected B95a cells, respectively. (d) Surface and
intracellular SLAM staining of B95a and B95a-MV cells.
response genes MDA5 and RIG-I31,32 were also examined because IFN has been suggested to be involved in the
establishment of persistent MV infection. MV RNA increased after 1 day of infection and then increased again
between 17 and 23 dpi, followed by a decline until 28 dpi (Fig. 3a). After this period, the vRNA level remained
low. Gene expression of IFN-β , RIG-I, and MDA5 were also induced in response to vRNA accumulation
(Fig. 3a). Although we were unable to accurately determine the percentage of the cells that were infected at 24 h
post-infection because of the syncytium, the virus infection clearly did not reach all of the cells at this time point.
Thus, the expression levels of MV N protein, IFN-β and IFN response genes that were normalised with gapdh
expression levels at 24 h post-infection (Fig. 3b) are probably underestimated. Nevertheless, the expression levels
of MV N protein and IFN-β at this time point were higher than those of cells in the persistent phase of MV infection. These data indicate that both IFN production and signalling pathways are functional in the early phase of
MV infection, prior to the establishment of persistence. When the persistent infection of MV is established after
28 dpi, the expression of these genes declines to basal levels, at which their expression is maintained. Thus, IFN
does not seem to be involved in the maintenance of persistent MV infection.
Expression levels of the IFN-β gene and IFN response genes were not impaired in B95a cells
persistently infected with MV. It has been reported that both IFN production and signalling pathways
are impaired by MV5,33. To investigate if IFN production and IFN signalling pathways were impaired in cells that
are persistently infected with MV, we examined the responsiveness of persistently infected B95a cells to poly I:C
stimulation. Poly I:C treatment induced the gene expression of IFN-β, IRF7, RIG-I, and MDA5 in B95a cells that
were persistently infected with MV as well as in uninfected control B95a cells (Fig. 4a–d). Thus, it appears that the
functions of vRNA-sensing and IFN signalling pathways are not impaired in these cells.
Prolonged MV N protein accumulation.
Next, we aimed to determine how MV restricts its own replication during persistent infection without causing any alterations in either the viral genome sequence or the
host antiviral response. We investigated this by monitoring the levels of intracellular viral proteins, and we began
by using a FACS analysis to examine the intracellular N protein expression because the N protein is the most
abundant viral protein. We found that the level of this protein was sustained during the establishment phase of
persistent MV infection (Fig. 5a). Thus, we next focused on the accumulation of other viral proteins. The accumulation of viral proteins that are required for replication was kinetically monitored by western blotting assays.
The expression levels of P and L proteins each increased at 1 and 23 days after infection and then declined quickly,
following the kinetics of vRNA levels (Figs 3a and 5b). The N protein level was sustained for a longer period than
the levels of P and L proteins and vRNA (Figs 3a and 5b).
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Figure 3. Viral RNA levels and IFN response during the establishment phase of MV persistent infection.
The ratios of MV N protein (a), MV-Trailer (b), ifnb1 (c), rig-I (d), and mda5 (e) to gapdh in three independent
infections are graphed. Horizontal bars indicate averages. Segmented y-axes are used in (a) and (b). (f) Relative
amounts of gapdh are graphed. The cell numbers were adjusted at the time of cell harvest, except at 1 dpi because
identical numbers of cells were inoculated the previous day.
Figure 4. RNA sensing in persistently MV-infected B95a cells. B95a cells and persistently MV-infected B95a
cells (B95a-MV) were stimulated with a complex of Poly I:C and Lipofectamine (Lipo) or with Lipo alone.
Twenty-four hours later, the mRNA levels of ifnb1 (a), irf7 (b), rig-I (c), and mda5 (d) were quantified by realtime RT-PCR. Ratios against gapdh are graphed. Error bars are SD (n = 3).
Overexpression of MV N protein interferes with MV replication.
Based on these observations
and the fact that N protein has known a binding capacity for P protein, viral genome, and cellular factors34, we
hypothesised that the over-accumulation of N protein competes with the viral genome for binding to the P-L
complex and to the cellular factors that are required for MV replication. To examine this possibility, we established 293SLAM cells stably expressing N protein with or without different levels of P protein (293SLAM-N,
293SLAM-NP clones #3, 6, 8, and 9; Fig. 6a) and then infected these cells with rMV-EGFP to monitor viral
replication. The viral replication was suppressed by the overexpression of N protein in 293SLAM-N cells, but
the expression levels of N protein in 293SLAM-NP clones #8 and 9 were not sufficient to block MV replication.
Although the expression levels of N protein in clones #3 and 6 were comparable to that in the 293SLAM-N cells,
the suppression of MV was restored by the co-expression of P protein (Fig. 6b). Thus, the accumulation of N protein to sufficiently higher levels than that of P protein causes a downregulation of MV viral replication.
Discussion
In this study, we presented one of the possible mechanisms by which MV establishes long-term persistent infection without mutations in the viral genome. During acute infection, MV induces IFN production and cell death.
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Figure 5. MV N, P, and L protein levels in MV-infected B95a cells. (a) Intracellular MV N protein was stained
at the indicated dpi. Open and filled histograms indicate uninfected and infected B95a cells, respectively.
(b) Western blotting of MV N, P, and L proteins at the indicated dpi. GAPDH was used as an internal control.
Averages of the relative protein amounts of N, P and L proteins at 23 dpi in three independent infections are
graphed.
Figure 6. Effect of MV N overexpression on MV replication. (a) Expression of MV N protein and P protein
in 293SLAM cells, stably MV N-expressing 293SLAM cells (293SLAM-MV-N), and 293SLAM cell clones
expressing MV N and P protein with different expression levels (293SLAM-NP clones #3, 6, 8, and 9). GAPDH
was used as an internal control. (b) 293SLAM, 293SLAM-MV-N cells, and 293SLAM-NP clones were infected
with rMV-EGFP at a MOI of 0.1. Representative fluorescence microscopy images of four independent infections
are shown.
The termination of the cytopathic effect and the IFN production in the persistent phase is achieved by a combination of receptor downregulation and viral replication restriction. Although moderate amounts of surface SLAM
downregulation by MV H protein have been reported35,36, we show here that long-term persistent infection with
MV induces a virtual disappearance of surface SLAM. MV is able to evade the innate immune system during the
persistent phase of infection by keeping vRNA levels lower than the host detection sensitivity for vRNA sensing.
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Low vRNA levels in the persistently infected cells also explain the observed reduction in infectious virion release,
a phenomenon that was also reported by earlier studies13, as well as the observations in clinical cases of an absence
of viremia in the late phase of MV infection and before the onset of SSPE23,37.
MV can keep its genomic RNA level constant in cells undergoing either a slow or fast cell cycle and can do so
even in resting cells, such as neurons38. In the present study, we found that N protein functions as a stabilizer of
vRNA during persistent infection. MV produced N protein during replication in persistently infected cells, and
we observed that the accumulation of N protein suppressed viral replication. In our proposed model, to avoid
losing the vRNA by cell division, replication could be restarted when the N protein is sufficiently diluted by
cell division. This model also provides a possible explanation for the previously reported observation that cells
persistently infected with MV are resistant to superinfection with a closely related virus but are not resistant to
superinfection with distantly related viruses13. As shown in this study, pre-existing N protein suppresses the replication of superinfected MV. Thus, N protein may also prevent the replication of closely related viruses, because
the N, P, and L proteins of MV are compatible among the Morbilliviruses, but they are not compatible with similar
proteins from other viruses39,40.
MV replicates exclusively in the cytoplasm, so most of the viral proteins localise to this site. However, the
cytoplasmic localisation of the N protein depends on the P protein because, in the absence of P protein, the N
protein localises to the nucleus41. Therefore, if the N protein levels are in excess of the P protein levels, the excess
N protein would be expected to translocate into the nucleus. Nuclear inclusion bodies composed of the N protein
are indeed observed in SSPE brain specimens and in cells persistently infected with MV41,42. These observations
support the over-accumulation of the N protein during persistent MV infection in vivo.
The type of persistent MV infection observed in the present study seems to be suitable for maintaining lifelong
protective immunity against MV. In acute infection, the virus replicates vigorously and induces IFN and immunogenic cell death until the MV receptor is downregulated and N protein accumulates to a sufficient level43. The
immune system then establishes a strong immunity against MV. During the persistent phase, the virus hides itself
from immune detection by restricting viral replication. Trace amounts of viral antigen may continuously stimulate the immune system, allowing the induction of lifelong protective immunity without the clonal deletion or the
cytotoxic T cell unresponsiveness induced by the presence of abundant antigen and the continuous IFN secretion
that is seen in chronic infection with lymphocytic choriomeningitis virus and hepatitis C virus44–46.
Methods
B95a and 293SLAM cells were described previously47,48. Anti-human
SLAM antibody (A12) was purchased from Biolegend. Alexa Fluor 488-conjugated anti-mouse and anti-rabbit
IgG antibodies were purchased from Life Technologies. Anti-GAPDH mouse monoclonal antibody was purchased from Millipore. Rabbit anti-MV N protein, anti-L protein serum, and monoclonal mouse anti-MV P
protein antibody were described previously49. Recombinant MV HL strain (rMV-HL) and rMV-EGFP were
described previously50. Briefly, the HL strain is a wildtype virus that uses SLAM and nectin-4, but not CD46, as
receptors. The rMV-EGFP was constructed by inserting an EGFP-coding sequence between the N and P genes of
MV-HL cDNA. These viruses were propagated and titrated in B95a cells49. The pCAGzc-MV-N was constructed
by inserting the coding sequence of the N protein from the HL virus strain into the EcoRI and NotI sites of the
vector pCAGzc51. The pCAGzc-MV-N with or without pCAG-MV-P was then transfected into 293SLAM cells
followed by selection with Zeocin for 293SLAM-MV-N and 293SLAM-N/P cells.
Cells, antibodies, and viruses.
Establishment of persistently infected cells. B95a cells were infected with the indicated viruses at a
multiplicity of infection (MOI) of 0.01. Culture medium was changed every 5 days until cells started to grow
without obvious syncytium formation and cell death. The cell numbers were counted, and 5 × 105 cells were inoculated into new culture dishes every cell passage from 17 dpi. The initial MV infections were performed in three
separate dishes, and the infected cells were then passaged independently.
Virus genome sequence analysis.
Total RNA was prepared from persistently MV-infected B95a cells.
Complementary DNA was synthesised with random primers and PrimeScript reverse transcriptase (Takara
Bioscience). The viral genome sequence was amplified in small segments covering whole virus genome by PCR
with Phusion High-Fidelity DNA polymerase (New England Biolabs). PCR fragments were directly sequenced
with BigDye Terminater v3.1 (Applied Biosystems) and 3130 Genetic Analyzer (Applied Biosystems). The resulting sequences were then compared with the original genome sequence of rMV-HL.
RT-PCR.
Total RNA was purified with Isogen (Nippongene) according to the manufacturer’s instructions.
Complementary DNA was synthesized with Prime script Reverse Transcriptase (TAKARA) and random hexamers. Real-time PCR analysis was performed with THUNDERBIRD SYBR qPCR Mix (Toyobo). For analysis of
the relative expression of each MV gene, cDNA was synthesised with oligo-dT primers and an MV full genome
plasmid55 was used as a standard. For detection of 5′ copy-back DI particle genomes, primers A, B, and C were
used for PCR. All of the primers used in this study are listed in Supplementary Tables S1 and S2.
Flow cytometry.
Surface SLAM was stained with anti-human SLAM antibody (A12; Biolegend) diluted
1:1000 in phosphate-buffered saline (PBS) supplemented with 2% foetal bovine serum (FBS-PBS), followed by
Alexa fluor 488-conjugated anti-mouse IgG (Life Technologies) diluted 1:2000 in 2% FBS-PBS. For intracellular
staining, cells were fixed and permeabilised in 70% cold ethanol on ice for 30 min. Cell were washed with PBS and
stained with anti-MV N protein rabbit serum and Alexa fluor 488-conjugated anti-rabbit IgG (Life Technologies).
Cells were analysed with a FACSCalibur machine (BD Bioscienses) and CellQuest software (BD Biosciences). For
cell cycle analysis, cells were incubated with 0.5 µg/ml of RNase A (Sigma-Aldrich) and 40 µg/ml of propidium
iodide (Sigma-Aldrich) at 37 °C for 30 min and then analysed by FACS.
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Western blot assays. Anti-GAPDH mouse monoclonal antibody was used as an internal control. A total of
1 × 105 cells were directly lysed in sodium dodecyl sulfate (SDS) sample buffer, and 20 µl of the lysates were loaded
into gels for SDS polyacrylamide gel electrophoresis (PAGE). MV L, N, and P proteins were separated on 8% and
10% SDS-PAGE gels and transferred onto polyvinylidene fluoride membranes (Millipore). MV N, P, and L proteins were detected with anti-MV N protein serum diluted 1:1000, anti-MV P protein antibody diluted 1:1000,
and anti-L protein serum diluted 1:200, respectively, in Can Get Signal immunoreactions enhancer Solution
1 (TOYOBO), followed by incubation with an appropriate secondary antibody conjugated with HRP (DAKO)
diluted 1:3000 in Can Get Signal Enhancer Solution 2. The resulting signals were developed with ECL prime (GE
Healthcare) and detected with a LAS4000mini (GE Healthcare).
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Acknowledgements
This study was supported by KAKENHI Kiban-S, grant number 22228005 from the Ministry of Education,
Culture, Sports, Science and Technology, Japan.
Author Contributions
T.D. and C.K. planned the research. T.D. performed the research. H.K., T.H., H.S., and M.Y. provided reagents and
advice. T.D. and C.K. wrote the manuscript. All authors reviewed manuscript.
Additional Information
Supplementary information accompanies this paper at http://www.nature.com/srep
Competing financial interests: The authors declare no competing financial interests.
How to cite this article: Doi, T. et al. Measles virus induces persistent infection by autoregulation of viral
replication. Sci. Rep. 6, 37163; doi: 10.1038/srep37163 (2016).
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