Volume 3 • 2015
10.1093/conphys/cov019
Research article
Colby D. Moore1,*, Andreas Fahlman2, Daniel E. Crocker3, Kathleen A. Robbins1 and Stephen J. Trumble1
1Department
of Biology, Baylor University, One Bear Place, Waco, TX 76706, USA
of Life Sciences, Texas A&M University Corpus Christi, 6300 Ocean Drive, Corpus Christi, TX 78412, USA
3Department of Biology, Sonoma State University, 1801 East Cotati Avenue, Rohnert Park, CA 94928, USA
2Department
author: Department of Biology, Baylor University, One Bear Place, Waco, TX 76706, USA. Tel: +1 254 710 2101.
Email: colby_moore@baylor.edu
*Corresponding
As marine divers, pinnipeds have a high capacity for exercise at depth while holding their breath. With finite access to oxygen,
these species need to be capable of extended aerobic exercise and conservation of energy. Pinnipeds must deal with common
physiological hurdles, such as hypoxia, exhaustion and acidosis, that are common to all exercising mammals. The physiological mechanisms in marine mammals used for managing oxygen and carbon dioxide have sparked much research, but access
to animals and tissues is difficult and requires permits. Deceased animals that are either bycaught or stranded provide one
potential source for tissues, but the validity of biochemical data from post-mortem samples has not been rigorously assessed.
Tissues collected from stranded diving mammals may be a crucial source to add to our limited knowledge on the physiology
of some of these animals and important to the conservation and management of these species. We aim to determine the reliability of biochemical assays derived from post-mortem tissue and to promote the immediate sampling of stranded animals
for the purpose of physiological research. In this study, we mapped the temporal degradation of muscle enzymes from biopsied Northern elephant seals (Mirounga angustirostris) and highlight recommendations for storage protocols for the best preservation of tissue. We also compared the enzymatic activity of different muscle groups (pectoral and latissimus dorsi) in
relation to locomotion and measured the effects of four freeze–thaw cycles on muscle tissue enzyme function. Results indicate that enzymatic activity fluctuates greatly, especially with varying storage temperature, storage time, species and muscle
group being assayed. In contrast, proteins, such as myoglobin, remain relatively continuous in their increase at 4°C for 48 h.
Stranded animals can be a valuable source of biochemical data, but enzyme assays should be used only with great caution in
post-mortem tissues.
Key words: Degradation, enzyme, myoglobin, pinniped
Editor: Steven Cooke
Received 19 December 2014; Revised 21 March 2015; accepted 11 April 2015
Cite as: Moore CD, Fahlman A, Crocker DE, Robbins KA, Trumble SJ (2015) The degradation of proteins in pinniped skeletal muscle: viability
of post-mortem tissue in physiological research. Conserv Physiol 3: doi:10.1093/conphys/cov019.
Introduction
Muscle tissue samples collected in vivo have provided a vast
amount of knowledge on the physiology, exercise performance
and basic muscle structure of marine mammals (Kanatous
et al., 1999, 2008; Dearolf et al., 2000; Watson et al., 2003;
Trumble et al., 2010; Kielhorn et al., 2013; Velten et al.,
2013). Research on marine mammal muscle tissue often
© The Author 2015. Published by Oxford University Press and the Society for Experimental Biology.
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/),
which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
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The degradation of proteins in pinniped
skeletal muscle: viability of post-mortem
tissue in physiological research
Conservation Physiology • Volume 3 2015
Research article
To determine the integrity of decomposed tissue and determine enzymatic activity and proteolysis over time with varying temperature regimens, skeletal muscle (latissimus dorsi;
LD) was tested in a controlled laboratory setting, at a standard storage temperature (4°C), room temperature (21°C)
and mammalian body temperature (37°C) for up to 48 h.
These temperatures were chosen based on their common use
in animal storage, post-mortem examination and transportation, respectively. Citrate synthase (CS), lactate dehydrogenase (LDH) and myoglobin (Mb) were chosen due to their
common use in marine mammal literature as proxies for
metabolic profiles (Castellini et al., 1981; Reed et al., 1994;
Kanatous et al., 1999, 2008; Polasek et al., 2006). We hypothesized that the stability of enzymes would be greater in standard storage (4°C) when compared with higher temperatures
(21 and 37°C) and that caution should be exercised when
using skeletal muscle tissue from stranded individuals for
2
enzymatic assays. In addition, we hypothesized that in order
to maintain muscle integrity, immediate cold storage is necessary even with the risk of repeated freeze–thaw, because these
conditions would be less detrimental than exposure to higher
temperatures for even short periods of time. To our knowledge, this is the first study using marine mammal tissue to
determine the degree and rate at which skeletal muscle
becomes unusable for physiological investigations.
Materials and methods
Animals
Skeletal muscle biopsies were obtained from five live adult
male Northern elephant seals (NES; Mirounga angustirostris;
n = 5). In addition, California sea lions (CSL; Zalophus
californianus; n = 2), one NES (n = 1) and one harbour seal
(Phoca vitulina; n = 1) were sampled immediately post-mortem. For a terrestrial mammal comparison, biceps femoris
skeletal muscle was extracted from a Rattus rattus (postmortem, but not upon immediate death). All samples were
immediately stored at −80°C for long-term storage, except
field samples, which were placed into a liquid nitrogen dry
shipper (Thermo Scientific) before they were transported
overnight to −80°C. Northern elephant seal muscle samples
were collected during muscle physiology research on Año
Nuevo State Reserve (CA, USA) during beach haul-outs in
2013. Seals were anaesthetized with an intramuscular injection of Telazol, a teletamine/zolazepam hydrochloride, at a
dose of ∼0.3 mg/kg (Crocker et al., 2012). Doses of ketamine
and diazepam were also administered intravenously as needed
to maintain immobilization (Fort Dodge Laboratories, Fort
Dodge, IA, USA; Crocker et al., 2012). Latissimus dorsi muscle was accessed via incision after sterilization of the outer
skin area (2 cm2 area). Biopsies (30–50 mg) were obtained in
the mid-belly of the muscle at identical locations in all NES,
using local Lidocaine® (1 ml; Whitehouse Station, NJ, USA)
and a 6 mm cannula (Depuy, Warsaw, IN, USA; Crocker et al.,
2012). Samples were collected under National Marine
Fisheries Service marine mammal permit #14636. All procedures were approved by Sonoma State University institutional
animal care and use committee, and every precaution was
taken to ensure that all biopsy samples were maintained in a
sterile environment from sampling through assay. Postmortem marine mammal samples were obtained from The
Marine Mammal Center in Sausalito (CA, USA) under permit
#932-1905-00/MA-009526.
Assay protocols
Northern elephant seal skeletal muscle was thawed specifically for assay and immediately subjected to two temperatures
(4 or 21°C) and four time intervals (3, 12, 24 and 48 h) during
decomposition studies. Skeletal muscle was homogenized
using a Bullet Blender (0.5 mm zirconium oxide beads; Next
Advance, Averill Park, NY, USA) in Sigma CellLytic MT buffer
(Sigma Aldrich).
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focuses on aerobic and anaerobic properties and capacities of
skeletal muscle deduced from biopsies (Lenfant et al., 1970;
Castellini et al., 1981; Kooyman et al., 1981; Reed et al.,
1994; Kanatous et al., 1999, 2002, 2008; Ponganis et al.,
2002; Burns et al., 2005; Noren et al., 2005; Richmond
et al., 2006; Clark et al., 2007; Spence-Bailey et al., 2007;
Hindle et al., 2009; Prewitt et al., 2010; Shero et al., 2012;
Moore et al., 2014). Obtaining marine mammal specimens for
research purposes is often difficult. Small tissue biopsies from
opportunistic captures during permitted research as well as
subsistence hunts are used in physiology research (Reed et al.,
1994; Kanatous et al., 1999; Polasek et al., 2006; Kanatous
et al., 2008). It is less common for publications to include
post-mortem tissue sampled from either bycaught or stranded
specimens. Between 1999 and 2014, there have been approximately twice as many published papers using biopsy sampling
in marine mammal research in comparison to post-mortem
specimens (Kanatous et al., 2002, 2008; Ponganis et al., 2002;
Burns et al., 2005; Noren et al., 2005; Richmond et al., 2006;
Clark et al., 2007; Spence-Bailey et al., 2007; Hindle et al.,
2009; Prewitt et al., 2010; Shero et al., 2012). The relative
importance of post-mortem tissues, whether from euthanized
stranded or bycaught animals, depends on whether intramuscular biochemical data collected from post-mortem species is
feasible for tissue-based related physiological research.
Previous studies using marine mammal tissues collected at
post-mortem examination 24–72 h after collection and up to
30 h after death have yielded publishable results (Moore et al.,
2009; Hoffman et al., 2013). Post-mortem samples collected
within 6 h of death from stranded animals have been shown
to complement data pertaining to physiological adaptations
to depth and pressure (Watson et al., 2003, 2007; Polasek
et al., 2006; Lestyk et al., 2009). After 6 h post-mortem, tissue
integrity may be compromised due to decomposition or from
sample handling or storage. There are a number of conditions
that contribute or add to proteolysis, such as enzymatic activity (Geesink et al., 2006), temperature (Morita et al., 1996),
disease state (Costelli et al., 2005), pH (Eijsink et al., 2005)
and level of muscle atrophy (Kachaeva and Shenkman, 2012).
Conservation Physiology • Volume 3 2015
The effects of four freeze–thaw cycles on skeletal muscle
enzyme concentration were examined in the rat muscle only.
Muscle was maintained at −80°C during the freeze event and
thawed repeatedly. In the course of one freeze–thaw event, muscle was frozen at −80°C, thawed completely (completed in a
matter of minutes in the small tissue samples), homogenized and
used in the citrate synthase enzymatic assay. Each freeze–thaw
cycle measurement was made 24 h apart; thus, the muscle was
fully refrozen before the subsequent measurement was made.
Lactate dehydrogenase assays were performed according
to the Sigma Aldrich protocol (MAK066) on a spectrophotometric multiwell plate reader (Beckman Coulter, DTX880).
The quantification of LDH was based on the catalysis of the
interconversion of pyruvate and lactate, which reduced NAD
to NADH and was detected at 450 nm. Briefly, and according
to Sigma Aldrich protocol (MAK066), protein samples were
mixed with LDH assay buffer and a master reaction mix containing buffer and LDH substrate. Samples were then rotated
between incubation at 37°C and measurements every 5 min
until activity surpassed the highest standard.
Myoglobin assays were completed using methodology
modified by Kanatous et al. (1999) from Reynafarje (1963).
Homogenates were diluted in phosphate buffer (0.4 m potassium phosphate at pH 6.6) and centrifuged at 28 000g for
50 min. The supernatant was bubbled with carbon monoxide
for 3 min before being measured for spectrophotometric
absorbance. Absorbance was measured at two wavelengths
(538 and 568 nm), and Mb concentration was calculated in
milligrams per gram of wet muscle mass.
Statistical analysis
Group differences were assessed using ANOVA followed by
Tukey–Kramer HSD test. Results were analysed with statistical significance at P ≤ 0.05 α level. Results are presented as
means ± SEM.
Results
Overall, there was an increase in citrate synthase activity
between samples maintained at 4 vs. 21°C, from 3 to 48 h
(Fig. 1; ANOVA, P < 0.05). The CS activity level was measured
for four adult male NES (Fig. 1 and Table 1). Measurements
were made over 48 h at five time points (0, 3, 12, 24 and 48 h)
at 4 and 21°C (Fig. 1). At each time point after 0 h, the 4°C
group had elevated enzymatic activity compared with the 21°C
group (Fig. 1; Tukey–Kramer HSD, P < 0.05). For biopsies
maintained at 4°C, CS enzymatic activity increased over time
up to 12 h (26.1 ± 3.5 μmol/min/g, Fig. 1; Tukey–Kramer
HSD, P < 0.05). The percentage change in CS activity level
(4°C) over four sampling time frames (0–3, 3–12, 12–24 and
24–48 h; Table 2) fluctuated from a 42.3% increase to a 46%
decrease, demonstrating the instability of this enzyme over
time. The percentage change in CS activity level at 21°C
decreased, with the largest negative percentage change from
0 to 3 h (−78.1%) in comparison to 21°C from 3 to 12 h
(82.4%). The CS activity level was also measured in a rat
Figure 1: Citrate synthase activity level (in micromoles per minute per
gram; mean values ± SEM) in the longissimus dorsi muscle of four
Northern elephant seal adult males. Measurements were made over
48 h at two different temperatures [black represents 4°C (individuals
ES13-M3 and ES13-M12) and grey 21°C (individuals ES13-M13 and
ES13-M4)], indicating the greater stability of the enzyme at 4 vs. 21°C
in biopsied muscle tissue. ES represents “elephant seal”, 13 is the year
of collection and “M” is male.
Table 1: Citrate synthase (in micromoles per minute per gram; mean
values ± SEM) and lactate dehydrogenase (in milliunits per millilitre;
mean values ± SEM) for five adult Northern elephant seals over 24
and 48 h at two different temperatures: 4°C [ES13-M3 (CS), ES13-M2
(LDH) and ES13-M12] and 21°C (ES13-M4 and ES13-M13)
Time
CS at 4°C
CS at 21°C
LDH at 4°C
LDH at 21°C
0h
13.3 ± 1.6
29.0 ± 1.0
48.9 ± 7.8
39.6 ± 4.5
3h
18.9 ± 3.0
6.3 ± 1.1
127.6 ± 44.7
54.1 ± 4.0
12 h
26.1 ± 3.5
11.6 ± 2.0
89.4 ± 9.5
50.6 ± 35.5
24 h
14.1 ± 2.8
9.4 ± 3.4
28.7 ± 12.6
30.4 ± 21.7
48 h
15.5 ± 4.2
9.7 ± 0.7
—
—
Abbreviations: CS, citrate synthase; LDH, lactate dehydrogenase. n = 5.
3
Q9
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Citrate synthase assays were performed on a Beckman
Coulter DU 730 spectrophotometer according to the Sigma
Aldrich protocol (CS0720). Briefly, and according to the
Sigma Aldrich protocol (CS0720), the activity level (in micromoles per minute per gram) was determined at a wavelength
of 412 nm by combining the protein sample, assay buffer, acetyl CoA solution, dithiobis-nitrobenzoic acid (DTNB) solution and oxaloacetic acid (OAA) solution. The reaction of
acetyl CoA and OAA to citrate followed the colorimetric reaction of DTNB to TNB, forming a yellow colour. The reaction
was followed for 1.5 min to measure the baseline. The OAA
was added, and after another 1.5 min the total activity was
measured. Results were based on the change in absorbance at
412 nm over 1 min and the extinction coefficient of TNB, as
outlined in the Sigma Aldrich protocol (CS0720).
Research article
Conservation Physiology • Volume 3 2015
Research article
Table 2: Percentage change in citrate synthase and lactate
dehydrogenase activity over four time frames at two temperatures
(4 and 21°C)
Time
frame (h)
Table 3: Citrate synthase activity level (in micromoles per minute per
gram; mean values ± SEM) for a rat after three freeze–thaw cycles
Freeze times
CS 4°C (%)
CS 21°C (%)
LDH 4°C (%)
LDH 21°C (%)
CS activity (μmol/min/g)
Baseline
14.3 ± 0.2
13.9 ± 0.5
0–3
42.3
−78.1
160.9
36.7
Freeze cycle 1
3–12
37.8
82.4
−29.9
−6.5
Freeze cycle 2
13.9 ± 0.6
12–24
−46.0
−18.6
−67.9
−39.8
Freeze cycle 3
14.3 ± 1.5
24–48
9.8
2.5
—
—
Abbreviation: CS, citrate synthase. n = 1.
Figure 2: Average citrate synthase activity level (in micromoles per
minute per gram; mean values ± SEM) in a rat locomotory muscle
measured in triplicate (n = 1). Measurements were made over 12 h at
two temperatures (black represents 4°C and grey 37°C), indicating the
greater stability of the enzyme at 4 than at 37°C.
locomotory muscle (biceps femoris; Fig. 2). The CS activity in
the rat muscle was elevated at 4°C (range from 16.0 ± 0.0 to
29.4 ± 0.5 μmol/min/g) when compared with 37°C (range
from 3.4 16 ± 0.5 to 16 ± 0 μmol/min/g; Fig. 2; Student’s
unpaired t-test, P < 0.05). Therefore, the CS activity level was
greater in both NES and rat tissues maintained at 4°C. For the
37°C rat muscle, CS activity was significantly decreased after
time 0 h (Fig. 2; Tukey–Kramer HSD, P < 0.05). The effects of
four freeze–thaw cycles on the degradation of rat skeletal muscle showed no statistical difference in CS activity when thawed
at 24 h. Therefore, the CS activity level was relatively stable
when the muscle was maintained at −80°C, even after four
consecutive freeze–thaw cycles (Table 3; Tukey–Kramer HSD,
P > 0.05).
The mean LDH activity level (in milliunits per millilitre of
extract) measured at times 0, 3, 12 and 24 h in four adult male
NES (Fig. 3 and Table 1) revealed a similar pattern to CS, in
that greater enzyme activity was evident at 4 than at 21°C
(Fig. 3). Overall, there was a statistical difference between animals maintained at 4 vs. 21°C, from time 0 to 24 h (ANOVA,
4
Figure 3: Lactate dehydrogenase activity level (in miliunits per
millilitre; mean values ± SEM) in the longissimus dorsi muscle of four
Northern elephant seal adult males. Measurements were made over
24 h at two different temperatures (black represents 4°C and grey
21°C), indicating the greater stability of the enzyme at 4 vs. 21°C in
biopsied muscle tissue.
P < 0.05). Therefore, the LDH activity level was higher in tissues maintained at 4°C over a 24 h period.
The change in Mb concentration as a function of time during decomposition was measured in one elephant seal
(ES3289) over 48 h at 4°C (Fig. 4 and Table 4). Myoglobin
values increased significantly from time 0 to 48 h (Fig. 4 and
Table 4; Tukey–Kramer HSD, P < 0.05). The average percentage increase in Mb over 48 h held at 4°C was 27.3% (Fig. 4
and Table 4).
Citrate synthase activity was measured in both the pectoralis major and LD muscle in four post-mortem marine mammals (with different time of death to sampling intervals)
stored at −20°C to compare the relative activity level between
the two muscles (Fig. 5). Both CSLs (CSL10281 and
CSL10305) demonstrated significantly increased levels of CS
activity in pectoral muscle compared with LD muscle
(ANOVA, P < 0.05). The elephant seal (ES3289) showed elevated levels of CS in the LD muscle (ANOVA, P < 0.05).
Citrate synthase enzymatic activity in the harbour seal
(HS2192) showed no significant difference in pectoral muscle
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Abbreviations: CS, citrate synthase; LDH, lactate dehydrogenase. n = 5.
Conservation Physiology • Volume 3 2015
Research article
Between time 0 and 24 h, tissues maintained at 4°C (ES13-M3,
ES13-M12) demonstrated a 6% increase in CS activity, whereas
the muscle tissues maintained at 21°C (ES13-M13, ES13-M4)
showed a degradation of 68% (from 29.0 ± 1.0 to 9.4 ± 3.4
μmol/min/g) over 24 h.
Table 4: Northern elephant seal (individual ES3289) myoglobin
concentration (in milligrams per gram; mean values ± SEM) over 48 h,
maintained at 4°C
Time (h)
Myoglobin (mg/g)
Rate of change over 48 h (%)
0
30.8 ± 0
—
3
35.1 ± 1.2
14.0
12
36.7 ± 0.7
4.6
24
38.8 ± 0
5.7
48
39.2 ± 0.7
1.0
n = 1.
vs. LD (ANOVA, P > 0.05). Therefore, for three of the four
marine mammals the primary locomotory muscle used (pectoralis in CSL and LD in NES) had elevated CS activity
(Fig. 5).
Discussion
Enzymatic assays, such as for CS and LDH, may not provide
reliable results, because the enzymes tend to be relatively
unstable over 24 h at both room (21°C) and refrigerator temperature (4°C). Myoglobin concentrations were more constant than either CS or LDH enzymes and showed a general
increasing trend (1–14%: Table 4). In addition, and unexpectedly, rat skeletal muscle enzyme (CS) concentrations were
not significantly different when assessed between freeze–thaw
intervals. These data indicate the necessity for immediate
(on-location) storage at temperatures below 0°C for skeletal
muscle tissue from both live and recently deceased animals.
Enzyme stability is related to a number of factors, including
pH, temperature and oxidative stress (Eijsink et al., 2005).
Lactate dehydrogenase is also sensitive to thermal conditions (Adler and Lee, 1999), with a 15% decline in activity
when stored at 4°C for 4 days (Jacobs et al., 1986) and 5% at
4°C after 24 h (Wagner et al., 1992). This study showed that
for NES skeletal muscle subjected to both 4 and 21°C for
24 h, LDH activity decreased by 41.3 and 23.2%, respectively
(Fig. 3 and Table 1). Although LDH was less variable at 4 than
at 21°C (Fig. 3), the increased variability of LDH at both temperatures indicates that enzyme data from muscle stored at
temperatures above freezing should be analysed with caution.
In addition, and much like CS, the variability among NES
adult males may indicate that LDH used as a proxy for metabolism may not be reliable.
In veterinary clinical cases, as with the response to stranding,
tissue biopsy samples are often collected and stored at different
temperatures for shipping and/or long-term storage (Stanley
et al., 2009). The repeated freeze–thaw of samples is unavoidable and may impact the integrity of samples. It has been
reported from studies in the food industry that the freeze–thaw
process can be detrimental to the overall quality of the tissue
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Figure 4: Northern elephant seal (individual ES3289) measured over
48 h at 4°C for degradation of myoglobin (Mb; in milligrams per gram;
mean values ± SEM). The figure indicates the increase of myoglobin
over time when maintained at 4°C.
When rat tissue was exposed to a high temperature (37°C),
the CS activity level decreased significantly compared with
4°C (Fig. 2), lending support to our hypothesis that temperature may cause a pronounced degradation of the CS enzyme
in NES. Our findings are in agreement with previous findings
that CS activity decreases at temperatures above 40°C (Zhi
et al., 1991) and inactivation of the enzyme is reached at 43°C
(Jakob et al., 1995). Thus, CS is very sensitive to thermal
stress (Jakob et al., 1995) and, depending on the environmental conditions at time of death and the storage temperature,
CS for aerobic determination stored in these conditions
should not be used. Citrate synthase activity varied among the
NES skeletal muscle samples at time 0 h, therefore, regardless
of time and temperature (Fig. 1). This correlates well with
previous research indicating that muscle tissue post-mortem
can be variable even within the same muscle group of the
same animal (Bendall, 1973). Given the large amount of variability among NES adult males (Fig. 1), the results suggest
that CS may not be a reliable enzyme as a proxy for aerobic
metabolism between animals, even in fresh biopsy samples.
Although freezing can affect the muscle tissue in various ways,
including formation of ice crystals, dehydration and denaturation of proteins (Jeong et al., 2011), the results from the present study suggest that freezing is the best method of
preservation. Evidence for frozen storage was demonstrated
by rat skeletal muscle repeatedly frozen four times, resulting
in no difference detected in mean CS activity among freeze–
thaw cycles (Table 3). However, some caution should be taken
with successive freeze–thaw cycles, because variability (SEM)
appeared to increase with increasing numbers of freeze events.
Research article
Conservation Physiology • Volume 3 2015
sample, due primarily to increased lipid oxidation and tissue
aesthetics with regard to its exposure to temperature alteration
(Tang et al., 2006; Jeong et al., 2011). Our data show that when
assessing the CS enzyme, valuable data can be obtained even in
the event that samples are thawed. No significant change in CS
enzyme activity occurs with successive freeze–thawing cycles.
Furthermore, tissue samples should be placed immediately into
−80°C, primarily because muscle storage over time and elevated
temperature can change enzymatic activity in as little as 3 h
even at refrigerator temperature (4°C; Table 1).
In the present study, we observed that Mb held at 4°C for
up to 48 h increased in a relatively constant manner. The Mb
concentration increased maximally by 8.4 mg/g (27%, time
0–48 h), compared with LDH, which fluctuated by 78.7 miliunits/ml (161%) at 4°C (Tables 1 and 4). In addition, the variability (SEM) associated with the Mb averages (0–1.2 mg/g)
was generally lower than SEM values for either CS or LDH
(1.6–4.2 μmol/min/g and 7.8–44.7 milliunits/ml, respectively;
Tables 1 and 4). While these changes in Mb would alter the
precision of muscle oxygen store measurements, they suggest
reasonable utility of post-mortem samples for defining species
differences, especially for species that are hard to sample.
During this study, we also detected that enzymatic activity
varied between different locomotory skeletal muscle groups
within the same animal (Fig. 5). To determine species specificity and differences in CS activity levels between skeletal muscle groups, CS activity was measured in the pectoralis major
and LD muscle in three individual pinnipeds representing different primary locomotion approaches; NES (hindflipper),
6
CSL (foreflipper) and harbour seal (hind-/foreflipper). The
greatest level of CS activity was found in the NES LD, the
primary locomotory muscle of this deep-diving phocid seal
(Le Boeuf et al., 2000; Kuhn et al., 2009; Robinson et al.,
2012; Fig. 5). For the CSL, a relatively shallow-diving Otariid
species (Feldkamp et al., 1989; Weise et al., 2006), the highest
CS activity was found in the pectoralis major when compared
with the CSL LD (Fig. 5). No difference was found between
CS activity in pectoralis major and LD muscles for the comparatively intermediate-diving phocid, the harbour seal
(Fig. 5). This may be indicative of the relative equal reliance
on fore- and hindflippers of the harbour seal vs. the elephant
seal or the relative lack of utilization of pectoralis major compared with the CSL. These CS activity data provide indirect
evidence designating which skeletal muscle group is the primary locomotory muscle in these marine mammal species
(Feldkamp, 1987). We suggest that CS enzymatic activity levels can provide data on the relative importance of individual
muscles to locomotion. Although enzymatic data may not
provide a reliable comparison across species or even within
species, we suggest that comparing between muscle groups
within the same individual provides valuable data.
The aim of this study was to quantify enzyme degradation
in marine mammal tissues collected post-mortem. We suggest
that our findings substantiate the expedited use of post-mortem
tissue and provide evidence that tissue is of greater value when
refrigerated or frozen immediately following removal from an
animal. When using enzyme assays to determine aerobic capacity, one may find that values have a large range, even among
biopsies from the same species and age class, potentially
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Figure 5: Citrate synthase activity level (in micromoles per minute per gram; mean values + SEM) in the pectoral and longissimus dorsi muscle of
four different marine mammals (indicated by the different alphanumerical codes), indicating the general trend for higher values in pectoral
muscle of those animals that predominately use the pectoral muscle for locomotion (California sea lions).
Conservation Physiology • Volume 3 2015
Acknowledgements
Thank you to the Crocker Laboratory at Sonoma State
University and the Kanatous Laboratory at Colorado State
University, Amy Rozzi, Ethan Pavlovsky, Dr Bryan Gibbon,
David Camejo, Dr Ken Wilkins, Dr Robert Doyle, Dr Bessie
Kebaara, Dr Darryn Willoughby, Baylor Department of
Biology, Texas A&M-Corpus Christi Department of Life
Sciences and The Marine Mammal Center.
Funding
This work was supported by the Office of Naval Research
[grant number N00014-12-1-0187].
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Conservation Physiology • Volume 3 2015