Desalination and Water Treatment
www.deswater.com
160 (2019) 94–109
August
doi:10.5004/dwt.2019.24194
Critical review on biological treatment strategies of dairy wastewater
G. Janet Joshibaa, P. Senthil Kumara,*, Carolin C. Feminaa, Eunice Jayashreea, R. Racchanaa,
S. Sivanesanb
a
Department of Chemical Engineering, SSN College of Engineering, Chennai 603110, India, Tel. +91 7395993423,
email: janujosh21@gmail.com (G. Janet Joshiba), Tel. +91 9884823425, email: senthilchem8582@gmail.com (P. Senthil Kumar),
Tel. +91 9003196798, email: feminacarolin@gmail.com (C.C. Femina), Tel. +91 9940168360,
email: eunice.jayashree@gmail.com (E. Jayashree), Tel. +91 9940338359, email: rracchana@gmail.com (R. Racchana)
b
Department of Applied Science and Technology, Alagappa College of Technology, Anna University, Chennai 600 025, India,
Tel. +91 9444960106, email: siva@annauniv.edu (S. Sivanesan)
Received 5 December 2018; Accepted 30 March 2019
ABST R AC T
Dairy products are one of the richest sources of vital nutrients in the diet of human beings and it
occupies an important place in satisfying their nutrient requirements. The dairy products possess a
very short lifespan and during their decomposition create a huge nuisance to the environment. The
dairy effluents discharged from the industries are mainly composed of complicated substances such
as organic compounds, inorganic compounds, carbon, nitrogen, phosphorus, chlorides, sulphides,
fats, oils, grease, etc. These organic loading present in the dairy effluent have a negative impact on
the environment during its discharge to nearby water sources. The physical and chemical treatment
of the dairy effluents is not as effective as the biological treatment. The biological treatment method is
found to be the superior method for treating the dairy effluent. The biological wastewater treatment
can be performed in two various conditions such as aerobic and anaerobic. The treatment methods
such as aerobic lagoons, activated sludge, sequential batch reactor, trickling filter, completely stirred
tank reactors, fluidized bed reactors, and anaerobic filters are some of the biological methods used in
dairy effluent treatment. This review article has investigated in detail regarding the environmental
impact of dairy effluents and their effective treatment using biological treatment technologies
Keywords: Dairy industry; Biological treatment; Aerobic; Anaerobic; Wastewater
1. Introduction
Water is a prodigious asset of nature and it is a fundamental component required for the basic functioning of all
living organisms in the universe. It has got tremendous
impact on the betterment of human life, nourishment production and in monetary advancements [1]. Most part of
the surface of the earth is concealed by aqua; on the other
hand around 98% of water sources present in the planet
earth is incompatible for drinking purposes due to the elevated levels of dissolved salts in it. The rest of streams are
partially inaccessible as it is clinched in the polar ice tops,
icy masses, permeable rocks and underground excavations.
Therefore, only a small proportion of clean water present
in lakes and waterways are open to human needs [2]. The
discharge of noxious industrial effluents and strong domestic waste into the aquatic ecosystem changed the quality
of the water and causes a deficit of drinking water [3].
The augmenting surge in the urbanization and industrial
sector altered the quality of freshwater and played a vital
role in water deficit by extensively expanding the rate of
contamination in the water sources. The shrinkage of water
sources had a serious negative impact on the growth of the
industries and it complicates the regular life of humans [4].
According to the reports of FAO 2009, it is stated that the
consolidation of elevated population growth, lesser agricultural land, increasing pollution, soil disintegration, varying
*Corresponding author.
1944-3994 / 1944-3986 © 2019 Desalination Publications. All rights reserved.
G. Janet Joshiba et al. / Desalination and Water Treatment 160 (2019) 94–109
weather pattern, industrial pollution, etc., are some of the
reasons for the major protein deficiency disorders in many
countries [5]. Industries are said to be the major contributors to the growing economy of a nation. On the other hand,
the outbreak of various kinds of industries into the environment caused water deficit which is known to be a dreadful
issue faced by people living all over the world. The wastewater with less contamination is subjected to wastewater
treatment and used for various purposes [6]. Wastewater
treatment is an another important source to produce water
by treating it economically and eliminating all the hazardous components from the effluent [7].
Food industry is one among the several kinds of commodious industries present in our modern environment. Especially, these industries utilize a huge amount of fresh water
for their manufacturing unit and they are one of the leading
industries which eliminate profluent vigorously. The dairy
industry is one such industry categorized under the food
industries. India is one of the dominant milk-producing
country and it positions first in milk production among
several other countries. Dairy industry is one of the noteworthy industry which discharges waste thrice the volume
of milk produced, also it gushes out a vast amount of processed water in the vicinity of 3.739 and 11.217 mil lion m3
of waste for every year [8]. This sector also increases the
economic status of India [9]. Dairy industry possesses one
of the major positions in Italy which contributes to about
6% of the total world’s dairy commodity production. The
dairy industry involves various process like cleaning, sanitizing, manufacturing, blending, heating and cooling from
where a bulk amount of organic load is produced as effluent
[10]. The wastewater eliminated from these kinds of industries comprises of the easily degradable lactose, along with
some complex substances such as proteins, fats and oils.
Other major substances such as nitrogen and phosphorus
are present in the effluents as orthophosphate and other
complex forms [11]. Effluent treatment is one of the essential unit in every manufacturing sectors and it is involved in
eliminating the harmful components present in the effluent.
This method is composed of several mechanical and biological operations which eliminate the organic, inorganic, suspended and dissolved compounds present in the released
effluents. The treatment techniques also help in eliminating
the pathogenic micro-organisms present in the industrial
discharge and reduce its toxicity so that it does not create
big consequences when discharged into water sources [12].
1.1. Dairy industry
From the very beginning, milk was considered as one
of the greatest source of vital nutrients out of all other food
products [13]. It is one of the nutritious liquid synthesized
and secreted by the mammary glands of female mammals.
As a result of the quick industrialization occurring everywhere throughout the nation, the number of dairies and
united enterprises are pointedly rising [14]. Majorly, the
milk is occupied by 87.5% of water and further, it is composed of 3.9% of fat, 4.8% of lactose, 3.4% of protein, 0.8%
of minerals and 13% of solid compounds [15]. Dairy industry plays an important role in producing a large amount of
sustenance handling wastewater in numerous nations. As
it consumes a large amount of water for the manufacturing
95
of the dairy products it remains as the biggest antecedent of
food industrial wastewater [7]. Europe is one of the major
continent which produce an immense amount of dairy
effluents and a typical dairy industry in Europe produces
around 500 m3 of dairy effluents as a major waste containing large amounts of fats, proteins and carbohydrates
[16]. A tremendous amount of fresh water is utilized in
the unit operations including sterilization, heating, cooling, pasteurizing, cleaning and all other process involved
in the preparation of dairy products. The impact of dairy
wastewater on the environment results in formulations of
stringent regulations on the release of industrial effluents
[17]. Several advancements made in the animal husbandry
lead to the progress in the dairy industry by increasing
the milk production in the cattle through various scientific
techniques. This development in the dairy industry helped
several countries to meet their population’s nourishment
requirements [6]. Elimination of the organic loading composed in the dairy wastewater is mandatory to lower the
harmful environmental consequence [18].
1.1.1. Characteristics of dairy wastewater
The composition of the organic compounds present in
the effluents differs according to the products manufactured. Usually, in the cheese manufacturing process, a high
amount of carbohydrates and protein are available in the
effluents, while, in the manufacturing of ghee higher level
of lipid is present in the effluent released [19]. The increase
of cheese production in the industries gradually raises the
dairy wastewater. Dairy industry consumes higher quantity of carbohydrates and proteins for the manufacturing
and washing practices, further, dairy wastewater produced
from these sectors is composed of higher fixation of nitrogen
and other complex organic matter [20]. Basically, the dairy
wastewater does not comprise of any highly toxic chemical
substances as other industrial effluents. On the other hand,
it is basically made up of a mixture of organic compounds
such as lactose, whey proteins, nutrients and fats which
causes bad odors and makes distress to the encompassing
populace during its degradation stage [4]. The flow rate and
ingredients of the effluents are not constant and they tend
to fluctuate based on the manufacturing and production
process [21]. The dairy wastewater is one of the wastewater
with high organic content like other food industry effluent.
In specific it is characterized by its elevated levels of chemical oxygen demand (COD) and biological oxygen demand
(BOD) which becomes a major problem to the water source
into which this wastewater is discharged [22]. The characteristics of the dairy effluents depend on the following
features such as industrial scale, processing types, type of
method, the efficiency of the method, process parameters,
type of operation, selection of equipment for cleaning, type
of waste discharged and cost required for treatment of
wastewater [23]. Dairy industry contributes a major part in
the production of a large volumes of industrial wastewater
containing high organic load which cannot be eliminated
easily [24]. Most of the governmental and ecological protection organizations have created many inflexible rules and
regulations to decrease the harmful effects of the effluents
discharged from the industries. The dairy wastewater is
seen with high turbidity along with a large amount of sol-
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G. Janet Joshiba et al. / Desalination and Water Treatment 160 (2019) 94–109
ids, further, they contain a less amount of dissolved oxygen
in it. The efficient treatment of dairy wastewater is an indispensable method to secure the freshwater bodies which
receive the dairy wastewater from getting damaged by
the noxious emission of nitrous oxide (NO) and ammonia
(NH3). Further wastewater treatment also saves the water
bodies from the eutrophication which damages the quality
of freshwater [20]. Color is a subjective trademark that can
be utilized to survey the general state of wastewater. The
darkening of wastewater is regularly because of the development of different sulfides, especially, FeS [7]. Generally,
dairy wastewater is white or yellow in colour [14]. The pH
is quantified as the negative logarithm of the hydrogen particle fixation. Its range is given between 0 to 14; under 7
being acidic and more than 7 is considered basic. The wide
portrayal in the pH estimation of gushing can influence the
rate of organic response and survival of different microorganisms [7]. Milk possesses alkalinity but sometimes it
becomes acidic during the fermentation process in which
the lactose from the milk is converted to lactic acid [14].
The environmental condition like temperature also plays a
major role in creating impacts in the dairy ingredients and
also the natural radiations occurring in water for a living
being occupying oceanic media. It relies on the season, time
inspecting, and so on. The water temperature assumes an
essential part in affecting the wealth of phytoplankton. The
water released from the dairy industry, which has higher
temperature, influences the land unfavourably. The temperature of wastewater may extend from 26.2–35.4°C [7].
BOD is characterized as the measure of oxygen required by
a microorganism to degrade the complicated organic compounds and converting it into simpler compounds. During
the degradation process, the oxidation of natural toxins
takes place and it is converted into carbon dioxide (CO2)
and water (H2O) with the support of microorganisms consequently bringing down the measure of BOD [7]. The COD
test decides the oxygen required for concoction oxidation
of natural issue without the assistance of solid synthetic
oxidant. COD is a test, which is utilized to quantify contamination of residential and modern waste. The waste is
estimated as far as the nature of oxygen required for oxidation of natural issue to deliver CO2 and H2O [17].
The total suspended solids (TSS) are the total volume of
organic matter present in the dairy wastewater in the suspended form. The TSS formed during the curd making sector and it mainly consists of the cheese whey in it [14]. Dairy
wastewater consists of the immense amount of nutrients in
the effluents. The dairy effluent remains as one of the major
cause for water pollution in several regions because of the
presence of enormous amount of organic loading which is
the best food source for micro organisms such as bacteria,
algae and fungi [25]. The chemical composition of some the
dairy effluents is described in the Table 1.
1.1.2. Environmental effects of dairy wastewater
The dairy effluents are mainly composed of several
components such as organic, inorganic, nutrients, suspended and several solid components. These components
present in dairy effluents are responsible for the bad odor
and turbidity of the wastewater [20]. The processing steps
involved in the manufacture of dairy products include
water consuming divisions such as tanks, cleaning storehouses, warm exchangers, channels and homogenizers
give rise to a lot of effluents with a high organic waste
composition. Basically, the effluents gushing out of all
the above units produces wastewater with high organic
load constituted by the high amount of COD, BOD, P, N,
FOG [26]. The organic wastewater eliminated from the
dairy industries is highly harmful to the ecosystem when
compared to the other toxic gaseous and solid waste. The
organic waste rushed into the fresh water sources damages
the features of the water in all aspects and it becomes one
of the considerable producers of wastewater when compared to other industries [24]. The processing and manufacturing sectors of the dairy industry devour a generous
amount of fresh water and it also produces a large amount
of wastewater containing enormous organic and nutrient
materials as waste. It is necessary to treat the dairy wastewater before disposal. Feasible water and wastewater management were administered to enhance the quality of the
wastewater and some efficient techniques are employed
in the manufacturing process to lessen the consumption of
freshwater [27]. The dairy waste on increased organic con-
Table 1
Characteristics of some of the dairy effluents released from the industrial sources
Wastewater type
pH
BOD (mg/ L)
COD (mg/ L)
TSS (mg/ L)
TDS (mg/ L)
Reference
Raw wastewater
Whey waste
Milk industrial wastewater
Aavin dairy industry washwater
Raw wastewater
Dairy waste
Dairy effluent
Cheese whey waste
Dairy effluent
Dairy effluents
Cheese waste
Cheese whey
5.5–7.5
4.1
11.70
6.4–7.1
7.1
7.2–8.8
5.5–10.5
–
6.07–7.10
350–600
20000
3000–8910
–
2800
1200–1800
200–3500
–
–
980–7500
300–1400
–
1500–3000
71526
5000–14250
2500–3300
5000
1900–2700
2–2.5
68814
1402.8–2133.8
680–4500
650–3000
45000–72000
250–600
22050
1420–3540
630–730
–
500–740
–
–
–
–
250–2700
–
800–1200
–
–
–
–
–
–
–
–
–
–
–
[6]
[103]
[34]
[106]
[22]
[78]
[19]
[107]
[108]
[109]
[11]
[61]
4–12
5.5–6.5
G. Janet Joshiba et al. / Desalination and Water Treatment 160 (2019) 94–109
centration becomes noxious to the living organisms such as
fish, aquatic plants and algae present in the water sources
[20]. Dairy effluents are composed of complicated organic
mixtures. During the decomposition of nutrient components present in the dairy effluents, the volume of the
dissolved O2 gets lowered leading to an anaerobic environment in the water sources. The emission of highly noxious
odor from the dairy effluents is one of the major hindrance
for the public residing near the water sources. The freshwater sources which are contaminated by the dairy waste
becomes one of the major birth places for several insects
and pests, furthermore, due to its stagnant nature it acts as
a major breeding region for mosquitoes which are causing
several harmful diseases such as chickenguniya, dengue,
malaria, yellow fever, etc. The casein is one of the main
compound eliminated from the dairy industry and when
the casein is subjected to degradation it is converted into
a highly foul-smelling black colored waste. This black colored sludge is harmful to the aquatic inhabitants and it
causes the death of aquatic organisms [20]. The contamination caused due to the industrial effluents ruins the fishing business, creates a shortage of fresh water and causes
harmful diseases [28]. The dairy effluents when blended
with the fresh water sources cause hormonal disorders in
the aquatic lives and human beings [29]. The ecological
effect of dairy effluents can be high, particularly because
of the extensive release of waste waters which contain high
natural issues and different supplements including N and
P. The release of dairy plant effluents to the water assets can
prompt annihilation of sea-going life and other marine animals, which can provide more nourishment for microbial
consortia and bring on additional O2 consumption.
1.2. Dairy waste water treatment
The dairy industries manufacture various products
such as milk, spread, yogurt, etc. and consume an immense
amount of water. In addition, it also produces a large quantity of wastewater with elevated levels of organic loading
and it is mandatory to eliminate this organic waste before
being discharged. Dairy wastes are easily prone to contamination and it gives a bad odour on degradation which
creates a major nuisance to the nearby living beings and
it may also contain pathogens emitted from the contaminated materials [20]. The dairy industry processed water
such as rinse water and white waters released during the
production mechanism bestow greatly to the large amount
of dairy effluent released into the environment [30]. The
degradation of the dairy effluent is complex and along
with the mechanical and physico-chemical techniques
the biological treatment enhances the deterioration of this
organic waste [31]. The technologies used for degrading
the dairy wastewater must be selected according to various process parameters and energy consumption is one
of the main factors to be considered during the designing
of treatment technology [32]. Water recovery during the
sustenance preparing part especially in the dairy industry
should be painstakingly investigated since there is a high
danger of potential defilement of the dairy items with the
treated wastewater [33]. Be that as it may, a few issues have
been found during the wastewater treatment of dairy effluents such as low sludge settle ability, issues in the corrup-
97
tion of fatty substance, low protection to stun loads and
challenges in the expulsion of supplements such as N and
P [36]. The dairy wastewater should be pre-treated before
subjecting to the wastewater treatment plant to enhance
the remediation efficiency. Initially, the wastewater is
screened physically for removing the solid particles and
dust particles present in the effluents released from the
industry using grit chambers and wire screens. Then, the
pH of the wastewater is determined and certain pH adjustments are done to the wastewater so that it can be directed
to the correct treatment plant according to their alkalinity.
Furthermore, the FOG present in the wastewater become a
great hindrance during the effluent treatment and so it is
necessary to remove them from the wastewater. The FOG
are treated and removed using floatation, gravity traps and
hydrolyzing using enzymes [17]. A few natural treatment
frameworks including oxygen-consuming and anaerobic
forms have been utilized for dairy wastewater treatment.
Notwithstanding, every one of these frameworks has its
own particular inconveniences caused by either high vitality necessity or solid operational trouble [37].
1.2.1. Biological treatment method of dairy wastewater
The biological method is a critical and necessary part
of any wastewater treatment plant that remediates wastewater from various sectors and it also helps in reducing
the harmful impact of wastewater on the environment.
This method seems to be a propitious method of treating
wastewater with high organic content. This method uses
microbes for degrading the high organic loading and other
toxic chemical substances [19]. It is basically divided into
two types such as an aerobic and anaerobic method. The
deterioration of organic compounds in the wastewater
using microbes in the presence of oxygen is known as an
aerobic treatment method [14]. The success of a biological
treatment unit depends on two factors such as the ability of
the microbes in the system to degrade the organic loading
present in the effluent and the competence of the solid-fluid
detachment of the biomass at the last phase of the treatment
strategy [38]. Most dairy handling plant squanders react to
the biological treatment in light of the fact that the predominant natural materials in such squanders are fat, lactose,
and protein, which are promptly debased by oxygen-consuming microorganisms [39]. Basically, the effluents from
a dairy industry are composed of elevated levels of COD,
BOD and other organic compounds. These parameters in
the dairy waste can be lowered effectively by biological
treatment. Biological treatment offers a cost-effective technique for dairy effluents and the treatment techniques are
getting upgraded day by day [34]. The variation in the different parameters of the contaminants present in the dairy
wastewater makes it difficult to be treated, so it should be
assessed on an individual plant premise [39].
1.2.2. Aerobic treatment of dairy wastewater
Aerobic treatment is the treatment of wastewater in the
presence of oxygen, while anaerobic is without oxygen.
These two terms are straightforwardly identified with the
sort of microbes or microorganisms that are engaged with
G. Janet Joshiba et al. / Desalination and Water Treatment 160 (2019) 94–109
98
the debasement of natural polluting contaminants in a given
wastewater. Accordingly, high-impact treatment forms
occur with only in the sight of air and the microbes utilize
this air to acclimatize organic contaminants which are then
converted to CO2, H2O and biomass. The natural treatment
is comprised of the oxygen-consuming aerobic and oxygen
starving anaerobic process. The aerobic treatment of dairy
wastewater is used for the diminishing of BOD and it is also
utilized for the evacuation of organic supplements such as
P and N. In the aerobic treatment, the wastewater is subjected to an oxidation reaction in the presence of oxygen
which leads to the deterioration of harmful microorganisms
residing in the diary industrial effluent [14]. The aerobic
treatment is commonly distinguished into two processes
such as suspended growth processes and attached growth
processes. Conventionally, many aerobic treatment methods such as aerated lagoons, oxidation pond, and activated
sludge process (ASP) are effectively used for treating the
organic industrial effluents [40]. Even though the aerobic
treatment seemed to be one of the efficient method to treat
dairy waste, the control of the air circulation administrations becomes a key issue [41].
1.2.3. Aerobic lagoons
Aerated lagoons are one of the finest technologies practiced for the treatment of dairy wastewater and it works as
a competent and easy strategy for evacuating the organic
and inorganic loading in the dairy effluents [42]. Lagoons
are one of the well-known treatment system used in the
treatment of dairy wastewater. In the beginning, it is used to
stock and treat animal waste in some parts of the world. The
lagoons can be operated both in aerobic and anaerobic condition depending on the type of wastewater released from
the industry. The schematic diagram of the aerobic lagoon
is illustrated in Fig. 1 [17]. The cost-effectiveness and their
efficient activity have made the aerobic lagoons a suitable
technique for treating the dairy effluents in many developing nations [42].
Many researchers have stated that the lagoons can be
efficiently used to lower the concentration of the nutrients and organic compounds in the industrial effluents.
NH3 is the major compound of nitrogen present in the aerated lagoons which complicate the effluent treatment [43].
Microorganisms residing in the aerated lagoons are mainly
responsible for the degradation of the organic and inorganic substrates present in the effluents. Bacteria is another
major category of prokaryotes present in the lagoons and
they highly support the degradation of the organic loading composed in the effluent. Several varieties of bacteria
are involved in the functioning of the aerated lagoons.
They help in converting the complicated organic and inor-
Fig. 1. Schematic representation of aerated lagoons [17].
ganic compounds into simple compounds which are easily
degradable [12]. The design and the functioning of the aerated lagoons are enhanced by understanding the different
parameters of the microorganisms such as type, structure,
morphology and nutrient uptake rate of the microorganisms
[12]. Temperature is considered one of the important criteria in the execution of aerobic lagoons because of its great
impact on the metabolism of the microorganisms living in
the lagoons [42]. The efficacy of the aerated lagoons mainly
depends on the type of living organisms acclimatized in
the lagoon and their nutrient uptake rate [12]. The maintenance of the removal efficiency of the aerated lagoons is bit
difficult as it mainly depends on the characteristics of the
microorganisms, to enhance the performance of the aerated
lagoons many new advancements have been implemented
in the lagoon system for the efficient elimination of the
toxic pollutants in the dairy effluents. Some of the aquatic
plants such as water hyacinth and duckweed are subjected
to the lagoon system for removing the elevated amount of
nutrient and organic loading [44]. The implementation of
duckweeds in the aerated lagoons seemed to increase the
removal of the organic content composed in the dairy effluents. The elevated concentration of the nutrients such as N
and P in the dairy effluents leads to eutrophication in the
lagoons. The average amount of N and P recovered from
the effluents using duckweed wetlands are seemed to be
in the range of 22.4 gN/m2/y and 7.4 g P/m2/y [43]. Luo
et al. [44] have conducted a study on removal of nitrogen
using Myriophyllum aquaticum in a three stage pilot scale
surface flow constructed wetlands. The nitrogen removal
efficiency was determined to be 87.7–97.9%, whereas the
total nitrogen content was obtained to be 85.4–96.1%. The
hazardous diseases caused due to the waterborne viral contaminations is one of the biggest limitations of the aerated
lagoon system and so to prevent the disease outbreaking
from the lagoons several precautions should be taken by the
people working near the pond and also the lagoons should
be maintained in a perfect condition [45].
1.2.4. Trickling filters
There has been a developing enthusiasm for the use
of trickling filters for the high-impact treatment of industrial waste waters [46]. Basically, in the trickling filters, the
wastewater is implemented on the biofilms developed on
the surface of the medium and the microorganisms residing in the biofilms consumes the organic compound in
the waste waters leading to degradation of complicated
organic substances in the dairy effluents. The trickling filters are not immersed into any medium the wastewater is
subjected into the media through the sprayers and this system is especially used to degrade the wastewater with high
organic and nutrient content [47]. The diagrammatic representation of the trickling filter is shown in Fig. 2 [17]. This
system is composed of a supporting medium along with
microorganisms with the provision for air circulation and
sprayer for distributing the wastewater [48]. Due to technological advancement various materials are used as media
for the growth of the microorganisms. Usually, the media
are arranged in distinct types such as vertical, tubular and
cross flow paths. The media is packed with greater surface
area, high void fraction and greater permeability [47]. The
G. Janet Joshiba et al. / Desalination and Water Treatment 160 (2019) 94–109
Fig. 2. Schematic representation of trickling filters [17].
efficiency of the trickling filter depends on the parameters
such as pH, temperature, volume of biomass, growth rate
of microorganisms, nutrient uptake and removal rate. The
trickling filter is known for removal of nutrients and nitrogen sources [49].
The wastewater with elevated levels of TSS, COD, and
BOD are treated using the trickling filters. In this method
the dairy effluents are subjected to the supporting media
covered by the biofilm and the organic wastes present in
the wastewater are consumed by the microorganisms present in the biofilms. The media is generally packed using
various materials such as rocks, plastic, foam, etc., [48].
The treatment of dairy wastewater using trickling filters
is seemed to be highly preferable due to its cost-effectiveness. The lava rock is used as a supporting medium in the
trickling filter and it aids in cleaning the waste compounds.
The media used in the trickling filters are selected based on
some of the parameters such as less weight, cost effectiveness, high strength, high shock resistance, high corrosive
resistance, elevated porosity and large surface area. The
microorganisms consume the vital nutrients such as C, H,
K and P; furthermore, it also contains a small number of
other elements. The organic loading present in the effluents
is transferred to the biofilms. The trickling filter column is
also packed with another cost efficient cheaper materials
such as wood wastes, peat, compost and ceramic. The consumption of wastewater increases the microbial population
leading to degradation of complicated organic substances
[49]. In the study conducted by Raj and Murthy [46] a cross
flow medium trickling filter utilized in the dairy wastewater treatment resulted in a higher COD elimination of about
1.94 kg m–3 d–1 at a hydraulic loading rate of about 17 m3 m–2.
Mehrdadi et al. [48] have investigated the efficiency of trickling filter on dairy wastewater and the results showed that
the microorganism present in the media biofilms effectively
eliminate COD of about 2.750 mg/L.
1.2.5. Activated sludge process
Activated sludge process (ASP) is a highly preferred
method for treating the waste waters with organic and inorganic loading; henceforth it is highly used in treating the
99
Fig. 3. Diagrammatic representation of activated sludge
process [17].
household and sewage waste waters. It is highly preferred
for the removal of carbon, nitrogen and ammonia compounds present in the dairy wastewater [42]. The schematic
representation of the ASP is shown in Fig. 3 [17]. ASP is one
of the highly preferred method for treating the dairy effluents due to its ability to remove and treat nutrients present
in the effluents [50].
Microbes and protozoa are main ingredients of activated sludge process which helps in degradation of nutrients and organic substances composed in the wastewater.
These microbes consume the suspended organic waste substances present in the wastewater and it converts them into
an activated sludge which is later on removed and recycled.
The understanding about the microbial groups and their
relationship with these organic substances and biological
wastewater treatment is needed to quickly screen and survey process exhibitions and to enhance the organic procedures happening in wastewater treatment plants. Protozoa
populaces assume a noteworthy part in the microbial sustenance networks amid the organic treatment in wastewater
treatment plants, Furthermore; they are generally utilized as
a marker of actuated muck plant execution [41]. Activated
sludge treatment is an effective aerobic used in the biodegradation of organic components present in the dairy wastewater. In this method, the wastewater is passed into a tank
provided with aeration and further the activated sludge is
removed from the wastewater using a clarifier giving out a
clarified wastewater [51]. This system is also highly effective in the degradation of complicated substances such as
protein, lactose, fats and oils using the microorganisms [41].
The ASP coalesced with the membrane reactor seems to be
an effective system for treating dairy wastewater. This system has shown great execution for the expulsion of organic
matter from the dairy wastewater [52]. The optimization and
determination of process coefficients are essential in designing an efficient activated sludge system. The ASP with 5 d
of incubation period along with maintenance of BOD at
95% shows an effective removal of organic compounds
from dairy effluents. This system functions beneficially
in the pH range of about 9–10.5 [53]. Activated granular
sludge is a notable advancement in the ASP, furthermore, in
this method a set of the novel microbial group works on the
expulsion of C, N, P and different organic compounds present in the effluent. The granular sludge system is entirely
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G. Janet Joshiba et al. / Desalination and Water Treatment 160 (2019) 94–109
different from the activated sludge system in all aspects
such as its morphological, chemical and biological properties. This system is well suited for cost effective treatment
of the dairy wastewater and it shows an efficient removal
of every nutrient and organic compounds present in the
dairy effluents [54]. The aerobic granular sludge treatment
is efficient than the normal activated sludge system due to
its strong resistance against shocks, elevated settle ability,
less toxicity and higher biomass recovery [55]. The aerobic granular sludge system is used to treat the industrial
effluent containing around 100–1000 mg L–1 of suspended
solids in the effluent. As the dairy industry is composed
of a large number of suspended solids, aerobic granular
sludge system is implemented for the treatment of the dairy
effluent [56]. Activated sludge system is used to treat the
wastewater with elevated levels of organic content and it
showed pretty good removal efficiency in eliminating the
waste waters with high COD, BOD, N, P and other nutrient
compounds [57]. In the treatment of dairy wastewater using
ASP conducted by Nasr and El-Kamah [28] the results concluded that nearly 64–96% of volatile organic compounds
(VOC) correspondingly.
1.2.6. Rotating biological contactors (RBC)
Rotating biological contactors (RBC) was initially used
in the year 1900 using wooden racks as the discs. Later on,
the polystyrene material was used as the discs in 1958 and
remarkable advancement have been made in the design of
the RBC. It primarily works on the adsorption principle and
it is generally used in the treatment of several industrial and
household waste waters [58]. RBC are highly preferred treatment system for removing the nitrogen compounds from
the industrial effluents [48]. In specific, the wastewater possessing higher BOD and COD is treated using the RBC. The
diagrammatic representation of RBC is viewed in Fig. 4 [61].
RBC system is widely preferred than other treatment system
due to its high removal efficiency and less energy consumption. It is an attached growth reactor includes a set of circular
discs rotating in a circular way in which basically the discs
are made up of plastic. The rotating discs with microorganisms feed on the nutrients and organic matter in the water.
Aeration is also provided to enhance the growth of the microorganism in a strong way [58]. RBC is one of the well-known
systems for its cost-effectiveness and higher shock resistance.
Usually, the rotating discs are immersed in the dairy effluents containing organic content and the surface of the disc
covered with the biofilm layer containing microorganisms.
The discs with the biofilm layer are submerged into the effluents and rotated serially. This further leads to the degradation of the organic content by the microorganism present
in the discs causing reduction of the organic and nitrogen
content. The microorganisms in the biofilm consume the
organic and nutrient compound leading to the increase in the
volume of biomass. The biofilm layer slowly gets thickened
and increases in volume. The thickened sludge falls from the
discs and gets segregated in the sludge digester where it is
converted into water and gas [59].
In RBC, the gaseous O2 is exchanged to the fluid film
in the part presented to air. This broke up gas at that point
diffuses to the biomass where it is expended alongside the
natural aggravates that also circulates into the biofilm. Amid
the submerged stage, the fluid film is incompletely peeled off
and it blends with the mass fluid as the circle leaves the fluid
pool [60]. Around 40–45% of the disc is submerged into the
dairy effluents with a suitable contact time. The rotating discs
are rotated in a correct speed without disturbing the biofilm
attached on the disc. The rotation of the disc helps in bringing
out the relationship between the air and water, furthermore,
the water rising due to the rotation of the disc are brought
back to the container. The cyclic rotation of RBC paves the
way for the biofilm to consume all the organic substances
present in the wastewater through which the nutrients and
organic matter get diffused into the biofilm layer [61]. RBC is
a natural technique to deteriorate the organic issue by acquiring it adequate contact of air. It offers an elective innovation
to traditional actuated slop process in light of its straightforwardness to keep up and work, having high process security
with less space prerequisite. RBC framework ends up being
temperate as tertiary evacuation isn’t required [60]. The RBC
are highly preferred for several effluent treatments due to
its features such as larger surface, less energy consumption,
cost-effectiveness, easy construction, less shock, less maintenance and easily operatability. Much technological advancement has been made in the RBC and it becomes one of the
main treatment systems in the aerobic treatment of wastewater [61]. RBC have shown higher removal efficiency than
other treatment due to its compatibility and greater reaction
rate [62]. Kadu et al. [63] have investigated the dairy effluent
treatment utilizing the three-stage rotating biological contactor. The results of this study concluded that higher BOD,
COD and TSS removal efficiency of about 96%, 80% and 79%
is obtained at a rotational speed of 8 rpm. The energy consumption in the RBC is nearly equal to the extended aeration
activated sludge process. Nearly 50% of the organic content
present in the dairy effluents are removed during the initial
stage of the RBC unit itself [63]. Ebrahimi and Asadi [61] have
investigated the treatment of dairy effluent using three stage
BC resulting in a greater COD removal efficiency of about
80–83% is obtained for an HRT of 16–24 h. In this study the
higher COD removal efficiency of 92% was seemed to be elevating with the increase in HRT level.
1.2.7. Sequencing batch reactor
Fig 4. Schematic representation of rotating biological contactors
[61].
Sequencing batch reactor (SBR) is a time specified reactor system and it functions in a serial mode repeatedly. The
G. Janet Joshiba et al. / Desalination and Water Treatment 160 (2019) 94–109
five stages of the SBR are filled, react, settle, decant and idle,
furthermore, the SBR works repeatedly in these five stages
in a cyclic manner [64]. SBR seems to be the most promising technology for the treatment of dairy wastewater. It
is a ‘fill and draw’ activated sludge system. The schematic
representation of the sequential batch reactor is viewed in
Fig. 5 [17]. In this system, wastewater is added to a single
batch reactor, treated to remove undesirable components,
and then discharged.
Equalization, aeration, and clarification can all be
achieved using a single-batch reactor. SBR is one of the
auspicious methods used for treating the effluents from
the dairy industry [39]. Basically, the SBR works under the
principle of activated sludge system. The SBR has shown a
successful execution sequestration of N, C, P, etc. Moreover,
the procedure followed in the SBR permits impersonating
of numerous procedures in traditional consistent stream
reactors and can yield unrivaled execution [34].
The wastewater with high total solid concentration is
treated effectively with the help of the SBR system due to
its efficiency in lowering the suspended solids concentration and cost-effectiveness [65]. The efficiency of the SBR
system depends on the parameters such as volume of dissolved O2, retention time, HRT, composition of organic
load, COD, nutrient composition, denitrification and nitrification rate. It is a compatible treatment system for many
industrial effluents due to its simple construction and efficiency [64]. The dairy wastewater containing high organic
compounds can be effectively treated in an aerobic granular
sludge SBR. The granular sludge is an important parameter
which the biomass retention is higher than other process
because the granular sludge settles down faster than activated sludge particles. The particulate components present in the dairy wastewater are adsorbed on the granules
and they are degraded in the SBR cycle [38]. The SBR system has defeated numerous hindrances and furthermore
contrasts from other treatment technologies. The SBR has
shown satisfactory results in treating the dairy wastewater
with different convergences of COD, BOD, TSS and other
Fig 5. Schematic representation of sequencing batch reactor [17].
101
organic loading content [34]. Dairy effluent using the SBR
has showed decreased levels of suspended solids. Furthermore, the cumulative treatment efficiency of SBR in treating
dairy effluents was found to be 90.8% for TSS, 86.5% for
BOD, 78.5% for COD, thus, the SBR is found to be highly
effective in managing the dairy wastewater [39]. The SBR is
preferred over other treatment technologies due to the features such as simple activity, easy in handling water inconstancy, minimal effort, no requirement for settling tank and
high removal efficiency of TS [65]. Abdulgader et al. [34]
have investigated the treatment of dairy wastewater using
sequencing batch flexible fibre biofilm reactor. In this work,
high COD parameters removal efficiency of about 89.7% and
97% have been reported. Bae et al., [52] have investigated
the elimination of nutrient using membrane separation process combined with an SBR. The results showed that higher
BOD removal of about 97–98% is obtained using the membrane and rest of the nutrients such as N and Pare excluded
at a removal efficiency of 80% using SBR. Mohseni-Bandpi
and Bazari [65] have investigated the treatment of industrial dairy wastewater using SBR. In this study, a higher
COD removal efficiency of more than 90% is achieved with
a COD concentration of about 400–2500 mg L–1.
2. Anaerobic treatment of dairy wastewater
Even though there are some upsides of the aerobic
method, there are some downsides involved in these studies. The major drawback in the aerobic treatment is utilization of high energy through aeration, less efficient due to the
enormous growth of microbes [66]. Anaerobic treatment is
the supportive method for treating organic constituents in
the dairy wastewater due their notable advantages. Anaerobic treatment of dairy waste waters is a complex process that
degrades the organic matter by the variety of intermediates
and utilizes microbial consortium in the absence of oxygen.
It is an important approach in the reduction of pollution
and waste [67]. This type of treatment is considered to be
an excellent process by several researchers when compared
with the aerobic process [68]. Degradation and stabilization
steps are involved in the anaerobic digestion process which
uses microbes under anaerobic conditions [69]. The types
of microorganisms involved in the anaerobic degradation
are mixed cultures of acidogenic and methanogenic bacteria which have varieties of optimal environmental conditions. Providing best environmental parameters for the
entire microbial group is troublesome. Hence improvement
of ideal conditions for the methanogenic bacteria contributes prosperous operation of anaerobic degradation. A few
elements including pH, temperature, organic loading rate
(OLR), HRT, C/N ratio, presence of inhibitory issues and
reactor arrangement may influence the execution of anaerobic systems [23,70]. The anaerobic process has attained
high relevance, since renewable energy can be produced
through the biological treatment of dairy wastewater [71].
Mostly maximum percentage of high strength organic load
gets changed into inert biological cells, CO2 and the rest is
used for cell development. For natural stabilization of dairy
wastes, anaerobic systems are observed as more economical because of low energy requirement in these systems.
Besides generation of sludge is low when compared with
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G. Janet Joshiba et al. / Desalination and Water Treatment 160 (2019) 94–109
the aerobic systems and utilize high content of organic waste
for energy generation [72]. Terrible flavours are away if the
framework is worked productively. Anaerobic treatment
assimilations have been perceived by the United Nations
improvement program as one of the better valuable source
of energy supply due to their less capital investment. For
the past two decades, various anaerobic reactors have been
designed in order to employ various routes of holding up
the biomass within the rectors [73]. Different sorts of anaerobic digesters were utilized as a part of research facilities
to treat dairy waste waters for example, anaerobic filters,
up flow anaerobic sludge blanket reactors [74], anaerobic
packed bed, anaerobic contact digesters and anaerobic fluidized bed reactors and so on [75]. Since this biochemical
process occurs by the consortium of microorganisms, their
population levels and their types gets affected easily due to
the interruption in system which directly affects the performance of reactors. The most important factors of anaerobic
reactors are sludge retention and mass transfer which helps
to achieve best efficiency in the wastewater treatment [76].
The anaerobic reactors retain highly active biomass that
enhances the contact between the waste and biomass. Thus
the high rate anaerobic reactors hold up the biomass for longer time than the HRT [77]. These anaerobic reactors have
several upsides than the aerobic like quick start up with
less issues, capacity to resist shock, need of low inorganic
nutrients, capacity to adjust discontinuous feeding, use of
unsophisticated instruments, low energy requirements, low
operational and capital costs and velocity of restart after
long close down periods [78–81]. The operational expenditure and capital expenditure of aerobic ad anaerobic reactors are explained in Table 2. This table shows that more
number of anaerobic reactors have less OPEX and CAPEX
when compared with aerobic reactors.
Anaerobic reactors generates biogas like CH4 and CO2
[82] and helps to transfer these gases to the other process
which are the additional advantages of anaerobic digestion
process. For the past few years the application of anaerobic
treatment has been raised in treating high quality wastewater with less energy requirement and a by-product production like biogas. Nowadays, the researchers have been
showed their interest towards anaerobic digestion because
this process emits some nutrients which can be recycled and
used as bio-fertilizer in the agriculture [66]. Several studies
were reported in this paper regarding the treatment of dairy
wastewater using different types of anaerobic reactors.
ment of varied waste waters. The major type of anaerobic
digestion process applied for the treatment of dairy wastewater is anaerobic filters. It is the most prevalent method
for treating low strength wastewater and it is limited for
high strength wastewater due to the high OLR [23]. Fig. 6.
represents the simplified diagram of anaerobic filter [14].
AF among the anaerobic procedures emerges as an appealing choice. The presence of filter media and their stability
in the anaerobic filters act as a physical obstacle across the
biomass [67]. It contain at least two filtration chambers in
their arrangement. In the AF, the organic material present
in the wastewater gets degraded by the biomass and then
adhered to the exterior of the filter material and additionally
these anaerobic filters are broadly utilized as an optional
treatment to enhance the solid removal. Utilizing suitable
packing media is vital to the AF because the physical and
chemical characteristics of the media have critical effect on
the biomass and its reactor performance.
AF are packed with different packing materials to hold
up the microbes in the voids. The activated microbes in
the filters make the reactor to perform with the high OLR
wastewater. Perfect packing media expands the surface
area and in addition porosity. An expansive surface zone of
the media improves the biomass connection and expanded
porosity diminishes the general reactor volume, and additionally limits obstructing of channel. Based on the supporting material in the anaerobic reactors, the removal of
organic materials from the dairy wastewater takes place.
The anaerobic filters have been directed by utilizing different materials like seashell, charcoal, plastic materials,
ceramic, sintered glass, and characteristic stones like limestone, rocks, pumice, clay and so on [23]. Some researchers
applied anaerobic filters in treating fruit canning and cheese
2.1. Anaerobic filters
Anaerobic filters (AF) are a delegate connected development process that has been broadly connected to the treat-
Fig. 6. Diagrammatic representation of anearobic filters [14].
Table 2
CAPEX and OPEX comparison of different bioreactors [72]
Treatment technique
CAPEX
OPEX
Merits and demerits
Lagoons
Anaerobic fluidized bed bioreactor
Upflow anaerobic sludge blanket
Membrane bioreactor
Low
Low
Low
High
Low
–
Low
High
Good resistance to hydraulic and organic shock loads
Low reactor volumes
Requirement of energy is low
Fouling
G. Janet Joshiba et al. / Desalination and Water Treatment 160 (2019) 94–109
dairy waste waters [73]. They observed that 80% COD
removal efficiency was achieved in these waste waters with
the maximum OLR of 19 and 17 g COD L–1d–1. Nowadays,
several researchers have been focussed on the utilization
of industrial by-products in the anaerobic filters for natural possible applications [83]. Jo et al. [84] study explained
that cheese whey and other substrates can be effectively
treated by the anaerobic filters which was packed with
blast furnace slag (BFS) obtained from the iron ore processing. In this experiment, they showed best COD removal of
about 80% OLRs of 0.8–2.4 g COD per day. Additionally,
kinetic studies also conducted for understanding the reactor system. Anaerobic filters have different impediments
like minimum reduction of nutrients, necessities of further
treatment of sludge and effluent. It was accounted be alternative growth treatment process for high quality effluent.
2.2. Up flow anaerobic sludge blanket (UASB)
In these current years, the utilization of up flow anaerobic sludge blanket has increased tremendous popularity
which is the most commonly used anaerobic reactors for
the treatment of waste waters containing organic waste
[85]. The schematic diagram of UASB reactor is illustrated
in Fig. 7. In 1970s, the unpacked reactor called UASB was
introduced for the treatment of wastewater. This reactor has
better settling characteristics because the sludge does not
undergo mechanical agitation. Appropriate mixing takes
place in UASB due to the flow distribution that occurs with
high velocity and through agitation it leads to gas production. Some of the components of UASB reactors are gassolid separator, influent entry system, effluent exit system
and sludge blanket [82]. This reactor is steady and vigorously process effective. When there is a need of cost reliable
method for the wastewater treatment, UASB plays a major
role. High volume of wastewater can be treated in UASB
which is found to be major upside of this anaerobic reactor [86]. In this reactor, the wastewater flows upward and
the cell gets held in the reactor due to the sludge settlement
within the reactor [87]. It converts the availability of chemical energy in wastewater into value added products and sol-
103
uble organics [88]. In a solitary reactor, UASB consolidates
the entire procedure of processing and settlement so that
the impression gets reduced. The conventional anaerobic
treatment process like UASB was applied for the treatment
of dairy wastewater. Vlyssides et al. [89] investigated dairy
wastewater using UASB reactor. This study interpreted that
this anaerobic digester was satisfactory due to the reduction of 90% COD in the dairy wastewater. Additionally, it
also demonstrated that the proposed innovation could be
supportable not just as far as the treatment of cheese dairy
wastewater yet for their energy usage too [89].
A newly modified UASB reactor was designed with
scum extraction device and lamella settler by some authors
[90] for the effective treatment of dairy wastewater. In this
study, they proposed that this system requires only fewer
processing units and operational costs. They also depicted
that modified UASB produces high volume efficiency and
lamella settler is more effeicient than the traditional method
like dissolved air flotation (DAF). In this new framework
generation of chemicals and energy consumption like traditional approach are kept away [90]. Gotmare et al. [91] used
UASB for treating organic loaded dairy wastewater and
analysed its performance through monitoring pH, COD,
BOD, TSS and volatile fatty acids (VFA) and biogas production. The reactor accomplished COD, BOD, TSS evacuation
proficiency was seen to be 87.06%, 94.50%, and 56.54% separately and VFA varies with the ratio 0.28–0.43. The overall
CH4 synthesis was observed to be 75% of the biogas [91]. In
spite of the fact that UASB has been noted for the focal points
in the ability of CH4 generation, low operational costs, high
toxin evacuation efficiencies and least creation of sludge, its
treatment productivity and its achievement is unsuitable
[92]. A study has been focussed on the performance of UASB
for treating the high fat content (1200 mg/L of FOG) of dairy
wastewater. They also evaluated the characterization of different biomass and concluded that this reactor showed high
COD removal rates of about 89–95% [93]. Rico et al. [94]
proposed a co-digestion process which consists of UASB
with a settler and water conversion framework. 95.1% COD
removal and a volumetric CH4 production rate of 9.5 m3 CH4
m–3 d–1 was obtained through this co-digestion process. They
described that this method reduces stability issues which
occurs by the alkalinity and this proposed technology are
more suitable for polluting environment with cheese whey
[94]. Table 3 depicts the COD removal obtained from various research articles which illustrates the performance of
UASB reactors. The sludge production also takes place in
Table 3
COD removal from the dairy waste using UASB reactors
Fig. 7. Schematic representation of up-flow anaerobic sludge
blanket reactor (UASB) [85].
UASB reactor
quantity (L)
Feed COD
COD
removal
References
31.7
12.85
8
10
1.5
6.6
120
700–1200 mg/L
500–4000 mg/L
19.4 Kg/ m3 d
4056 mg/L
700 mg/L
500–3300 mg/L
1000–2000 mg/L
55–93%
96.3%
95.1%
79.4–85.5%
90%
67%
95%
[110]
[111]
[94]
[111]
[93]
[112]
[18]
104
G. Janet Joshiba et al. / Desalination and Water Treatment 160 (2019) 94–109
the UASB with the organic and inorganic constituents along
with the microorganisms. Another drawback is that higher
concentration of solid content in the dairy wastewater also
affects the performance of the UASB.
after by some type of biomass separator. ACP depicts the
contact between high biomass concentration and small
reactor [14]. The return of extra sludge to the reactor
enhances the high contact between biomass and waste
which is the major benefit of the anaerobic contact process. So, high efficiency can be obtained from the recycling
of sludge. Due to the anaerobic bacteria, gas formation
occurs which leads to the poor sludge settlement which is
found to be drawback of ACP in dairy wastewater treatment. The generation of gas in ACP can be limited by
applying vacuum degasification and thermal shock before
the settler [98]. Though the ACP was found to be simpler
in concept than the other anaerobic reactors its operation
is difficult. Fig. 9 depicts the schematic image of anaerobic
contact process [14].
2.3. Continuous stirred tank reactor
It is widely practiced in the laboratory studies than the
full scale treatment because of its HRT limitation. Continuous stirred tank reactors are applied in treating synthetic and
diluted dairy wastewater [95]. Fig. 8 represents the schematic
picture of continuous stirred tank reactor (CSTR). Nowadays
researchers have been focussed on this reactor for the degradation of organic matter and high methane yield [96].
They used four types of laboratory scale CSTR. The
total reactor volume is 15 L and 10 L of head space. About
477% the CH4 production was raised in per unit reactor
volume and additionally (OLR) was increased from 1.83 to
5.04 g VS L–1 day–1. But CSTR are finite to the full scale treatment because of its limitation in HRT. Table 4 represents
different experimental conditions used in CSTR for the treatment of wastewater. Another investigation was carried out
by the researchers to identify the treatment limits in feed rate
and OLRs in continuous stirred tank reactor [97]. From the
result they have analysed that the complications in anaerobic
digestion of cheese whey can be settles out by the co-digesting with liquid dairy manure. The reliable process operation
was achieved in the reactor after 7–8 D of HRT.
2.5. Anaerobic fluidized bed reactors (AFBR)
As a result of loss of biomass during the reactor operation and due to the high quantity of solid in the effluent,
the reactor efficiency gets limited [82]. Hence the reactors
have been created with the additional support that adhere
the biomass which leads to small HRT and high loading
capacities. The Fluidized bed reactors (FBR) overcome this
problem and proved high mass transfer characteristics. The
schematic representation of laboratory scale FBR is shown
in Fig .10. It also has the capacity to deal with the high OLRs
[72]. In order to handle the surface solids in the effluent FBR
are generally utilized because it occupies large biomass concentration than other reactors. In an AFBR microorganisms
get adhered to the support media and the wastewater flows
upward to the over support media. The transporter media
incorporate plastic granules, sand particles, glass beads,
mud particles, and charcoal pieces etc. The transporter
2.4. Anaerobic contact process (ACP)
It is similar to the anaerobic activated sludge process
included with completely mixed anaerobic reactor taken
Fig. 9. Schematic representation of anaerobic contact process [14].
Fig. 8. Schematic representation of continuous stirred tank reactor (CSTR) [105].
Table 4
Experimental conditions of CSTR in the treatment of wastewater
Type of wastewater
Cheese whey wastewater
Cheese whey
Cheese whey and dairy manure
Dairy cow solid waste
Volume (L)
6
25
26.6
–
pH
7
–
–
5.5
Temperature (°C)
55
35
34
37
HRT (D)
10
18
10
6
Loading rate
–3
0.95 Kg m D
4.9 gL–1·D –1
–
–
References
–1
[113]
[114]
[115]
[116]
G. Janet Joshiba et al. / Desalination and Water Treatment 160 (2019) 94–109
105
Fig. 10. Schematic representation of anaerobic fluidized bed reactor [105].
medium is continually kept in suspension by capable distribution of the fluid.
The AFBR has been effectively shown for biosolids processing and excellent in handling of high content of solids
because of its improved mass and heat exchange rates and
the equal distribution within the liquid phase [99]. The
effectiveness of the FBR depends on certain components
like high contact between liquid and fine particles carried
out by microbes and has the capacity to avoid the growth
of biofilm thickness. Additional care was required for the
start-up of an AFBR when compared with the other types
of anaerobic reactors. The benefits of AFBR are generating
high biomass, high mass transfer rates, low space requirements, high flow rates, less clogging and capacity to handle
high shock loads [14,100]. Moreover, it gives high surface
area and good hydraulic circulation. Sowmeyan and Swaminathan [101] used inverse fluidized bed reactor for the
treatment of high strength wastewater. It accomplished 84
% COD removal from 35 kg m–3 d–1. The supporting materials employed in this reactor are perlite which provides better surface area and high biomass attachment. Finally, this
system achieved excellent OLRs and COD removal rates
and implies acceptable support to the deviations in OLR
and HRT. The disadvantages in working with AFBR include
pumping power requirement, lengthy start-up time, wasting of bio-growth etc. [100].
2.6. Anaerobic packed bed digester (APBR)
Anaerobic packed bed reactor was first introduced by
Young and McCarty in the wastewater treatment system
and it is related to the trickling filters that contains support material in the biofilm form [14]. Anaerobic packed
bed reactor (APBR) is an efficient method for treating dairy
wastewater. It also removes the waste without the process
failure though the wastewater has different variations. The
schematic illustration of APBR is depicted in Fig. 11 [14].
An appropriate APBR is one of the growth system which
depends on the principle of arresting of microbes in the
carrier media through entrapment and attachment in the
support media and additionally it has the ability to hold
up the biomass along with the support, increase the contact
between biomass and wastewater and furthermore biogas
generation and hence it has been widely used for the treat-
Fig. 11. Schematic representation of anaerobic packed bed reactor [14].
ment of dairy wastewater [102]. The operational mode of
APBR is up flow and down flow mode [81]. Wastewater
flows from the top or bottom to make up flow and down flow
arrangements. The most significant aspect in APBR design
is the choice of support material like ash, volcanic stones,
zeolite, wooden blocks, microorganisms, plastics, polymers, granular activated carbon and other polysaccharide
materials acts as a carrier material in packed bed digester
that remains in contact with the dairy effluent. A laboratory
scale study was carried out using APBR by Deshannavar et
al. [78] packed with polypropylene pall rings for the treatment of dairy effluent at the lower HRT of 12 h, at OLR of
5.4 kg m–3 D –1. The COD removal was shown to be 87%.
These results depicted that the APBR has high feasibility
in treating the wastewater. Researchers applied this system
into both laboratory and pilot scale with a material like tire
rubber and zeolite. They are highly efficient in operating
under the conditions like temperature (22–26°C) and HRT
(3 d) [102]. APBR give high surface area for the adhesion
of bacteria which leads to the prevention of wash out of
microbes form the reactor. Based on the porosity, the performance efficiency of APBR varies because high porous materials found to be more efficient than non-porous materials.
APBR have several advantages like simplicity of operation,
high mass transfer rates and high efficiency. Non-stop operation of APBR gives inconsistent product. The anaerobic
procedure has various points of interest like the generation
of biogas as an energy source which comprises of 50–70%
CH4. Patil et al [80] carried out a study using two up flow
anaerobic packed bed reactor (UAPBR) for the treatment of
cheese whey wastewater which consists of proteins, fats,
lactose and so on. The result of the work shown that based
on HRT, the COD removal was achieved about 94–96%. The
optimum control of temperature and pH indicated the generation of higher biogas production [80]. Deshpande et al.
[103] has made an attempt to analyse the performance of
UAPBR to treat whey dairy waste. They evaluated that the
COD value was found to be 42–200 mg L–1 and concluded
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G. Janet Joshiba et al. / Desalination and Water Treatment 160 (2019) 94–109
that the dairy wastewater is more suitable for the production of methane gas in packed bed reactor.
A study was conducted by Qazi et al. [77] for the conversion of organic constituents in the dairy effluent into biogas. The supporting media utilized in this reactor are foam
cubes, bamboo rings, fire bricks, PVC rings and gravels. The
percentage removal of COD, BOD and VSS were identified
and then found to be 96%, 93% and 90%, respectively. Certain
researchers used UAPBR which was operated under mesophilic conditions (37–45°C) for the treatment of dairy wastewater. They described that the increase of HRT, the removal
percentage of COD and BOD was increased along with the
biogas production [104], Hence from the outcomes, they concluded that this method is suitable for degrading the dairy
wastewater. APBR system is delicate for high strong substance waste waters that make obstructing of bearer materials and decreases stability of the materials [105].
be implemented in bringing out the organisms with good
degradation capacity. The energy consumption should
be decreased by using some of the alternative techniques
and the alternative energy sources should be used in the
treatment plants to support the energy consumption. The
aerobic treatment is an outstanding treatment technology
for removing the organic and fatty compounds in the dairy
effluents. The high energy consumption and air circulation
is a well known limitation of the aerobic treatment technology. The anaerobic treatment method is effective in treating the high organic content of the dairy wastewater using
digesters and UASB reactors.
References
[1]
[2]
2.7. Conclusion and future trends
Dairy industry is an industry producing food products
with great source of nutrients. The dairy industry produces
food items such as milk, cheese, curd, whey, etc. The effluent released from the dairy industry is composed of organic,
inorganic and fatty substances which are the major sources
of pollutant to fresh water sources. The organic loading of
the dairy industry when mixed with the fresh water sources
causes eutrophication and oxygen depletion in the marine
environment which lead to the aquatic life damage. The
wastewater treatment is mandatory to decrease the harmful
consequences of the dairy effluents. Aerobic and anaerobic
techniques are the two types of biological treatment method.
The biological treatment method of the dairy effluents is an
extraordinary technology for treating the dairy wastewater. This effluent consists of some complicated organic and
chemical components in it, the degradation also becomes
difficult. In the preliminary treatment process of wastewater, only few particles such as solids, fats, oil, grease, etc,.
can be excluded from the dairy wastewater. Rest of the high
organic loading of the dairy effluents are treated only by
the biological method. In the aerobic method, the fats and
supplements could be expelled in any case without much
of a stretch; high vitality necessity is an essential downside
due to the supply of air circulation. Nowadays, many new
techniques have been blended with the biological treatment
to provide a significance removal of organic content from
dairy wastewater. Distinctive blends of reverse osmosis,
nanofiltration, and ultra filtration with each other or potentially with organic and additionally substance strategies
are likely to end up new time of future research. Anaerobic
method is most generally utilized for treating dairy waste
waters, prevalently UASB and anearobic digesters. The
UASB are most generally utilized also, suitable for treating
dairy industry waste waters, since they can be treat extensive volumes of influents in a generally short time frame. Be
that as it may, these procedures somewhat debase wastewater containing fats and supplements as dairy wastewater. Along these lines, further treatment is fundamental for
anaerobically-treated dairy wastewater. The micro organisms of good removal capacity and of higher growth rate
should be implemented to decrease the time consumption of dairy wastewater. The genetic engineering should
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