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Production and Characterization of Tailor-Made Polyhydroxyalkanoates by Bacillus cereus FC11

Production and Characterization of Tailor-Made Polyhydroxyalkanoates by Bacillus cereus FC11

Pakistan journal of zoology
Abdul Hameed
Abstract
Polyhydroxyalkanoates (PHAs) are non-toxic, biocompatible, piezoelectric, and biodegradable thermoplastic. This study was designed to isolate the bacterial strains from trinitrotoluene (TNT)-contaminated soil capable of giving PHAs at high yields with tailor-made compositions. The isolated bacterial strain B. cereus FC11 gave PHA yield at 76.40 wt% using 500 mL Erlenmeyer flasks each containing 200 mL synthetic medium in the presence of glucose as a sole carbon source at pH value 7.0, 30 degrees C and 150 rpm after 48 h of incubation under submerged fermentation. Furthermore, tailor-made PHA compositions were synthesized by B. cereus FC11 by adding different co-substrates along with glucose in the medium. High performance liquid chromatography analysis revealed that the retention time of crotonic acid (by-product) obtained from digested PHA was approximately 24.97 min which was similar to the retention time of crotonic acid (by-product) obtained from the digestion of commercially av...

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